Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| [congenital human cytomegalovirus infection: value of human cytomegalovirus dna quantification in amniotic fluid]. | a quantitative pcr assay (rs elosa cmv, lambdatech) was used to quantitate hcmv dna in maternal amniotic fluid of 12 fetuses with congenital infection (group 1) and of 10 fetuses without congenital infection (group 2). hcmv detection was performed for both groups using culture and qualitative pcr. histologic examinations of fetal tissues and placenta were carried out for 9 patients from group 1. the amniotic fluid viral loads were negative in all patients of group 2. in group 1, all viral loads ... | 2002 | 11937445 |
| absence of ie1 p72 protein function during low-multiplicity infection by human cytomegalovirus results in a broad block to viral delayed-early gene expression. | human cytomegalovirus (hcmv) ie1 deletion mutant cr208 is profoundly growth deficient after low-multiplicity infection of primary fibroblasts. previously, we showed that many fewer cells infected with cr208 at low multiplicity accumulated the delayed-early (de) protein ppul44 than accumulated the immediate-early 2 (ie2) p86 protein, indicating a high frequency of abortive infections. we now demonstrate that accumulation of all de proteins tested was defective after low-multiplicity infection in ... | 2002 | 11932411 |
| lack of tumorigenesis in the mouse liver after adenovirus-mediated expression of a dominant stable mutant of beta-catenin. | mutations in the glycogen synthase kinase 3beta (gsk3beta) phosphorylation sites of the beta-catenin gene exon 3 are found in 20-30% of human primary hepatocellular carcinoma (hcc), whereas mutations in the apc or axin genes are found in other hcc populations. these data strongly suggest that the wnt signaling pathway is involved in hepatocarcinogenesis. to determine the role of beta-catenin in intestinal tumorigenesis, we earlier constructed a mutant mouse strain catnb(lox(ex3)), in which exon ... | 2002 | 11929813 |
| human cytomegalovirus inhibits maturation and impairs function of monocyte-derived dendritic cells. | dendritic cells (dcs) play a pivotal role in the generation of virus-specific cytotoxic t-cell responses, but some viruses can render dcs inefficient in stimulating t cells. we studied whether infection of dcs with human cytomegalovirus (hcmv) results in a suppression of dc function which may assist hcmv in establishing persistence. the effect of hcmv infection on the phenotype and function of monocyte-derived dcs and on their ability to mature following infection with an endothelial cell-adapte ... | 2002 | 11929782 |
| human cmv-igiv (cytogam) neutralizes human cytomegalovirus (hcmv) infectivity and prevents intracellular signal transduction after hcmv exposure. | pretreatment of human cytomegalovirus (hcmv) with human hyperimmune globulin (cytogam) in human embryonic lung (hel) fibroblast culture showed successful inhibition of infectivity, and decreased extracellular viral titers and extracellular viral dna. cytogam prevented hcmv from inducing intracellular activation of nf-kappab, sp-1, and p13-k signaling pathways and the production of immediate-early (ie), early (e), and late (l) viral proteins. cytogam neutralization of hcmv in this cell culture mo ... | 2001 | 11926746 |
| protective immunity against lethal hsv-1 challenge in mice by nucleic acid-based immunisation with herpes simplex virus type-1 genes specifying glycoproteins gb and gd. | dna-based vaccines were employed to assess protective immunity against herpes simplex virus in experimental infections of hairless (strain skh1) and balb/c mice. mice were vaccinated with plasmids containing the herpes simplex virus type-1 (hsv-1) glycoprotein b (gb) or d (gd) genes under the human cytomegalovirus immediate-early promoter control. vaccines were injected intramuscularly (i.m.) or intraperitoneally (i.p.) as purified dna alone or as formulations supplemented with different non-ion ... | 2002 | 11926742 |
| dendritic cells cross-presenting viral antigens derived from autologous cells as a sensitive tool for visualization of human cytomegalovirus-reactive cd8+ t cells. | cd8+ t lymphocytes are essential to contain viral infections, such as human cytomegalovirus (hcmv). to visualize these clinically important effector cells, we used dendritic cells (dc), which efficiently present exogenous antigens by mhc class i molecules (cross-presentation). | 2002 | 11923708 |
| opinion article: cytomegalovirus is a risk factor in atherogenesis. | whether or not infectious agents are involved in the pathogenesis of atherosclerosis has been a matter of discussion for the past 2 decades. although there is no definite proof of a causal role of human cytomegalovirus in atherogenesis, a body of knowledge supports the concept that this virus is involved in the development of atherosclerotic lesions. this review assesses the most important data published in support of this hypothesis. | 2002 | 11916496 |
| emergence of multiple drug-resistant human cytomegalovirus variants in 2 patients with human immunodeficiency virus infection unresponsive to highly active antiretroviral therapy. | in 2 patients infected with human immunodeficiency virus (hiv), highly active antiretroviral therapy was unable to suppress hiv replication as a result of the emergence of drug-resistant hiv variants. human cytomegalovirus (hcmv) retinitis developed in both patients, and an unusually complex mixture of drug-resistant hcmv variants was detected in both patients. | 2002 | 11915006 |
| mechanisms of replication of alpha- and betaherpesviruses and their pathogenesis. | the diseases caused by herpes simplex virus (hsv) and human cytomegalovirus (cmv) differ and distinct differences in biological properties of these viruses can be noticed at laboratory work. despite of this, the structure of dna and the replication cycle of both viruses shows remarkably common features. analogous proteins encoded by both viruses, act at initiation of viral dna transcription, at viral dna synthesis, at nucleocapsid formation and envelopment. on other hand, considerable difference ... | 2001 | 11901707 |
| mutations in the ul97 orf of ganciclovir-resistant clinical cytomegalovirus isolates differentially affect gcv phosphorylation as determined in a recombinant vaccinia virus system. | mutations in the human cytomegalovirus (hcmv) ul97 phosphotransferase have been associated with ganciclovir (gcv) resistance due to an impairment of gcv monophosphorylation. vaccinia virus recombinants (rvv) were generated that encoded different hcmv ul97 proteins (pul97) with mutations previously detected in resistant hcmv clinical isolates at codons 460, 520, 592, 594, 595, 598 and 607. these rvvs allowed quantification of gcv phosphorylation catalyzed by the different mutated pul97s. when com ... | 2002 | 11888658 |
| novel immediate-early protein ie19 of human cytomegalovirus activates the origin recognition complex i promoter in a cooperative manner with ie72. | the major immediate-early (mie) gene of human cytomegalovirus (hcmv) expresses ie86, ie72, ie55, and ie18 mrna by differential splicing. reverse transcription-pcr with ie72-specific primers generated an 0.65-kb cdna from hcmv-infected fibroblast rna, which does not correspond to any known mie cdna. nucleotide sequencing revealed that the 0.65-kb cdna is from exons 1, 2, and 3 and part of exon 4, indicating that it is derived from a novel alternatively spliced mrna of the mie gene. the cdna encod ... | 2002 | 11884540 |
| [human cytomegalovirus inhibits the proliferation of cfu-mk in vitro]. | to investigate the effect of human cytomegalovirus (hcmv) on the proliferation of colony forming unit-megakaryocyte (cfu-mk). | 2001 | 11877064 |
| trail and its receptors in the colonic epithelium: a putative role in the defense of viral infections. | tumor necrosis factor-related apoptosis-inducing ligand (trail) is a member of the tumor necrosis factor family and induces apoptosis by cross-linking either of the 2 trail receptors containing a death domain (trail-r1 or trail-r2). trail-r3 and trail-r4 are receptors that do not transmit an apoptotic signal. the aim of this study was to investigate the expression and function of trail and its receptors in normal colonic epithelium. | 2002 | 11874999 |
| [research on the oncogenesis of cervical epithelial cells co-induced by human papillomavirus and human cytomegalovirus]. | the carcinogenesis of the human cervical carcinoma is known closely associated with human papillomavirus (hpv). the purpose of this article is to identify whether another sexually transmitted factor, human cytomegalovirus (hcmv) plays as a co-factor role in the carcinogenesis. | 1999 | 11869507 |
| construction of a rationally designed human cytomegalovirus variant encoding a temperature-sensitive immediate-early 2 protein. | we generated a set of cysteine-to-glycine mutations and screened them to identify a temperature-sensitive allele of the human cytomegalovirus ul122 gene, which encodes the immediate-early 2 transcriptional activating protein. the mutant allele contains a single base pair substitution at amino acid 510. in transcription activation assays, the mutant protein activated the simian virus 40 early and human cytomegalovirus ul112 promoters at 32.5 degrees c but not at 39.5 degrees c. we constructed a m ... | 2002 | 11867756 |
| visualization of the er-to-cytosol dislocation reaction of a type i membrane protein. | the human cytomegalovirus gene products us2 and us11 induce proteasomal degradation of mhc class i heavy chains. we have generated an enhanced green fluorescent protein-class i heavy chain (egfp-hc) chimeric molecule to study its dislocation and degradation in us2- and us11-expressing cells. the egfp-hc fusion is stable in control cells, but is degraded rapidly in us2- or us11-expressing cells. proteasome inhibitors induce in a time-dependent manner the accumulation of egfp-hc molecules in us2- ... | 2002 | 11867532 |
| human cytomegalovirus enhances chemokine production by lipopolysaccharide-stimulated lamina propria macrophages. | to elucidate the role of mucosal macrophages in intestinal human cytomegalovirus (hcmv) disease, primary lamina propria macrophages (lpm) were isolated from normal human jejunum, infected with hcmv, and studied for their cytokine responses. hcmv infection of lpm was confirmed by the presence of hcmv ie72 (ul123), pp65 (ul83), and glycoprotein b (ul55) proteins, which were detected by immunofluorescence, beginning at postinfection (pi) day 3, and were sustained through pi day 12 in 0.1%-0.5% of l ... | 2002 | 11865414 |
| sumo-1 modification of human cytomegalovirus ie1/ie72. | human cytomegalovirus (hcmv) immediate-early protein ie1/ie72 is involved in undermining many cellular processes including cell cycle regulation, apoptosis, nuclear architecture, and gene expression. the multifunctional nature of ie72 suggests that posttranslational modifications may modulate its activities. ie72 is a phosphoprotein and has intrinsic kinase activity (s. pajovic, e. l. wong, a. r. black, and j. c. azizkhan, mol. cell. biol. 17:6459-6464, 1997). we now demonstrate that ie72 is cov ... | 2002 | 11861864 |
| distinct glycoprotein o complexes arise in a post-golgi compartment of cytomegalovirus-infected cells. | human cytomegalovirus (cmv) glycoproteins h, l, and o (gh, gl, and go, respectively) form a heterotrimeric disulfide-bonded complex that participates in the fusion of the viral envelope with the host cell membrane. during virus maturation, this complex undergoes a series of intracellular assembly and processing events which are not entirely defined (m. t. huber and t. compton, j. virol. 73:3886-3892, 1999). here, we demonstrate that go does not undergo the same posttranslational processing in tr ... | 2002 | 11861856 |
| development and evaluation of an internally controlled semiautomated pcr assay for quantification of cell-free cytomegalovirus. | quantification of circulating human cytomegalovirus (hcmv) is useful in clinical contexts such as virological surveillance of bone marrow transplant recipients and monitoring of antiviral therapy. this report describes an internally controlled, quantitative, semiautomated, hcmv genome assay that was developed primarily to measure hcmv dna in the plasma of severely leucopaenic patients. it exhibits greater sensitivity, wider dynamic range and higher sample throughput than a number of previously d ... | 2002 | 11857531 |
| sequence analysis of ul54 and ul97 genes and evaluation of antiviral susceptibility of human cytomegalovirus isolates obtained from kidney allograft recipients before and after treatment. | the frequency of infections caused by drug-resistant cytomegalovirus (cmv) in solid-organ transplant recipients is not known. only a few resistant strains have been described in transplant recipients. antiviral susceptibility to ganciclovir (gcv) and foscarnet (pfa) of cmv isolates from 24 renal transplant patients with cmv viremia and cmv disease before and after therapy were investigated by a solid phase elisa. the cmv dna polymerase (ul54) and viral phosphotransferase (ul97) genes were also s ... | 2001 | 11844151 |
| terminally repeated sequences on a herpesvirus genome are deleted following circularization but are reconstituted by duplication during cleavage and packaging of concatemeric dna. | the mechanisms underlying cleavage of herpesvirus genomes from replicative concatemers are unknown. evidence from herpes simplex virus type 1 suggests that cleavage occurs by a nonduplicative process; however, additional evidence suggests that terminal repeats may also be duplicated during the cleavage process. this issue has been difficult to resolve due to the variable numbers of reiterated terminal repeats that the herpes simplex virus type 1 genome can contain. guinea pig cytomegalovirus is ... | 2002 | 11799198 |
| us2, a human cytomegalovirus-encoded type i membrane protein, contains a non-cleavable amino-terminal signal peptide. | the human cytomegalovirus us2 gene product targets major histocompatibility class i molecules for degradation in a proteasome-dependent fashion. degradation requires interaction between the endoplasmic reticulum (er) lumenal domains of us2 and class i. while er insertion of us2 is essential for us2 function, us2 lacks a cleavable signal peptide. radiosequence analysis of glycosylated us2 confirms the presence of the nh(2) terminus predicted on the basis of the amino acid sequence, with no eviden ... | 2002 | 11790769 |
| [viral screening of organ donors and human cytomegalovirus seroprevalence in the hungarian population]. | the authors investigated 998 organ-donors for human cytomegalovirus seroprevalence. the donors were divided into three age-groups. in organ-donors the seroprevalence was found to be 84%. a study was also conducted on a fourth group consisting of 200 residents from an old-age home. the youngest donor was 2 years of age, the eldest old-age home resident was of 92 years. the examined persons represent the hungarian population. it was found that as the result of the investigation of all 1198 subject ... | 2001 | 11778361 |
| identification of glycoprotein gptrl10 as a structural component of human cytomegalovirus. | human cytomegalovirus (hcmv) has a coding capacity for glycoproteins which far exceeds that of other herpesviruses. few of these proteins have been characterized. we have investigated the gene product(s) of reading frame 10, which is present in both the internal and terminal repeat regions of hcmv strain ad169 and only once in clinical isolates. the putative protein product is a 171-amino-acid glycoprotein with a theoretical mass of 20.5 kda. we characterized the protein encoded by this reading ... | 2002 | 11773418 |
| immune reactivity of human sera to the glycoprotein b of human herpesvirus 7. | the glycoprotein b (gb) is highly conserved among distinct human herpesvirus 7 (hhv-7) strains. similarly to other herpesvirus glycoproteins, gb has been assumed to induce a specific human immune response. however, it did not appear as an immunodominant protein in conventional immunoblot assays. recombinant gb, obtained from either escherichia coli or baculovirus expression systems, did react specifically with hhv-7-seropositive sera, and the main corresponding epitopes were located in its n-ter ... | 2002 | 11773091 |
| nested polymerase chain reaction in the diagnosis of congenital cytomegalovirus infection. | human cytomegalovirus infection is highly prevalent in indian population. it is the commonest congenitally acquired infection causing various anomalies. the diagnosis of infection in neonates is difficult as igm may not be detected in all cases. the polymerase chain reaction is reported as alternative and better option in these patients. however, there is lack of data to substantiate this preference in a resource poor country like india. | 2001 | 11770239 |
| dietary titanium and infant growth. | dietary titanium as tio2+ improved animal growth during infancy while inhibiting the metabolism of intestinal bacteria. tio2+ was also found capable of inhibiting human cytomegalovirus in tissue culture. these and other findings indicate tio2+ improves infant growth by acting as an antibacterial and antiviral agent. the behavior of tio2+ stands in contrast to that of tio2, which is inert. | 2001 | 11762532 |
| aggressive periodontitis associated with fanconi's anemia. a case report. | fanconi's anemia is an autosomal recessive disease associated with chromosomal breakage as well as pancytopenia, skin pigmentation, renal hypoplasia, cardiac defects, microcephaly, congenital malformations of the skeleton, hypogonadism, and increased risk of leukemia. the present report describes the periodontal clinical and microbiological status of an 11-year old male having fanconi's anemia. | 2001 | 11759873 |
| a rapid monitoring system of human herpesviruses reactivation by lightcycler in stem cell transplantation. | to establish a practical monitoring system of human herpesviruses reactivation in patients undergoing stem cell transplantation, we developed a new, very rapid, highly sensitive, and quantitative pcr assay for accurate measurement of human cytomegalovirus (cmv), human herpesvirus 6 (hhv-6) and epstein-barr virus (ebv) dna using lightcycler. the lightcycler system revealed that there was a linear correlation in the wide range of viral template dna at the indicated number of pcr cycles. peripheral ... | 2001 | 11753554 |
| characterization of a human cytomegalovirus with phosphorylation site mutations in the immediate-early 2 protein. | a human cytomegalovirus mutant (tnsubie2p) was constructed with alanine substitutions of four residues (t27, s144, t233, and s234) previously shown to be phosphorylated in the immediate-early 2 (ie2) protein. this mutant grew as well as the wild type at both low and high multiplicities of infection. the mutant activated the major immediate-early, ul4, and ul44 promoters to similar levels, and with similar kinetics, as wild-type virus. however, the tnsubie2p mutant virus transactivated an endogen ... | 2002 | 11752183 |
| u(s)3 protein kinase of herpes simplex virus 1 blocks caspase 3 activation induced by the products of u(s)1.5 and u(l)13 genes and modulates expression of transduced u(s)1.5 open reading frame in a cell type-specific manner. | the coding domain of the herpes simplex virus type 1 (hsv-1) alpha22 gene encodes two proteins, the 420-amino-acid infected-cell protein 22 (icp22) and u(s)1.5, a protein colinear with the carboxyl-terminal domain of icp22. in hsv-1-infected cells, icp22 and u(s)1.5 are extensively modified by the u(l)13 and u(s)3 viral protein kinases. in this report, we show that in contrast to other viral proteins defined by their properties as alpha proteins, u(s)1.5 becomes detectable and accumulated only a ... | 2002 | 11752164 |
| treatment of malignant gliomas with a replicating adenoviral vector expressing herpes simplex virus-thymidine kinase. | we evaluated the interaction between oncolytic, replication-competent adenoviral vectors and the herpes simplex virus-1 thymidine kinase (hsv1-tk) gene/ganciclovir (gcv) suicide system for the treatment of malignant gliomas. we constructed a panel of replication-competent adenoviral vectors in which the luciferase (ig.ad5e1(+). e3luc) or hsv1-tk gene (ig.ad5e1(+).e3tk) replace the m(r) 19,000 glycoprotein (gp19k) coding sequence in the e3 region. ig.ad5e1. ig.ad5.clipluc and ig.adapt.tk are e1-d ... | 2001 | 11751394 |
| gcv resistance due to the mutation a594p in the cytomegalovirus protein ul97 is partially reconstituted by a second mutation at d605e. | a ganciclovir (gcv)-resistant human cytomegalovirus (hcmv) was isolated from an aids patient. molecular analysis of the hcmv ul97 gene revealed two point mutations, a594p and d605e, respectively. in order to evaluate quantitatively the impact of the individual mutations on gcv phosphorylation, recombinant vaccinia viruses (rvvs) were generated carrying either the two mutations (rvv-594/605) or only one mutation (rvv-594 or rvv-605, respectively). in cells infected with the rvv-594/605 double mut ... | 2002 | 11750939 |
| atpase activity of the terminase subunit pul56 of human cytomegalovirus. | herpesviral dna packaging is a complex process resulting in unit-length genomes packed into preformed procapsids. this process is believed to be mediated by two packaging proteins, the terminase subunits. in the case of double-stranded dna bacteriophages, the translocation of dna was shown to be an energy-dependent process associated with an atpase activity of the large terminase subunit. in the case of human cytomegalovirus it was not known which protein has the ability to hydrolyze atp. in thi ... | 2002 | 11744697 |
| multiplex real-time nasba for monitoring expression dynamics of human cytomegalovirus encoded ie1 and pp67 rna. | the monitoring for hcmv mrna expression in whole blood provides an accurate and informative diagnostic approach. | 2002 | 11744429 |
| novel baculovirus dna elements strongly stimulate activities of exogenous and endogenous promoters. | a dna sequence upstream from the polyhedrin gene of baculovirus autographa californica nucleopolyhedrovirus (acmnpv) was found to activate strongly the expression of full or minimal promoters derived from acmnpv and other sources. promoters tested included the minimal cmv (cmvm) promoter from human cytomegalovirus, the full heat shock 70 promoter from drosophila, and the minimal p35 promoter from baculovirus. deletion and mutagenesis analyses showed that this functional polyhedrin upstream (pu) ... | 2002 | 11741907 |
| the human elongation factor 1 alpha (ef-1 alpha) first intron highly enhances expression of foreign genes from the murine cytomegalovirus promoter. | in order to develop a highly efficient mammalian expression vector, we constructed a vector by the combination of the murine cytomegalovirus (mcmv) immediate early (ie) promoter and the human elongation factor one alpha (ef-1 alpha) first intron. the mcmv ie promoter was several fold stronger than the human cytomegalovirus (hcmv) immediate early (ie) promoter and the human elongation factor one alpha (ef-1 alpha) promoter in various mammalian cell lines such as nih3t3, neuro-2a, 293t or ht1080 a ... | 2002 | 11738725 |
| laboratory diagnosis of common herpesvirus infections of the central nervous system by a multiplex pcr assay. | a sensitive multiplex pcr assay for single-tube amplification that detects simultaneous herpes simplex virus type 1 (hsv-1), herpes simplex virus type 2 (hsv-2), varicella-zoster virus (vzv), human cytomegalovirus (cmv), and epstein-barr virus (ebv) is reported with particular emphasis on how the method was optimized and carried out and its sensitivity was compared to previously described assays. the assay has been used on a limited number of clinical samples and must be thoroughly evaluated in ... | 2001 | 11724856 |
| application of pcr in situ method for detection of human cytomegalovirus (hcmv) dna. | present hcmv diagnosis is relatively slow and inefficient. we applied pcr in situ to 15 samples of human salivary glands, 20 cytospins from blood, and 10 human fibroblast samples in order to detect the presence of hcmv dna. the results indicate that pcr in situ is an effective and very sensitive method for detection of hcmv infections in variety of specimens. | 2001 | 11720312 |
| membrane-specific, host-derived factors are required for us2- and us11-mediated degradation of major histocompatibility complex class i molecules. | human cytomegalovirus encodes two glycoproteins, us2 and us11, that target major histocompatibility complex (mhc) class i heavy chains for proteasomal degradation. we have developed a mrna-dependent cell-free system that recapitulates us2- and us11-mediated degradation of mhc class i heavy chains. microsomes support the degradation of mhc class i heavy chains in the presence of us2 or us11 in a cytosol-dependent manner. in vitro, the glycosylated heavy chain is exported from the microsomes. a de ... | 2002 | 11717308 |
| declining levels of rescued lymphoproliferative response to human cytomegalovirus (hcmv) in aids patients with or without hcmv disease following long-term haart. | to investigate the lymphoproliferative response (lpr) to human cytomegalovirus (hcmv) in two groups of aids patients undergoing long-term highly active antiretroviral therapy (haart): group 1 ( n = 22) with nadir cd4(+) cell count <50/microl and no hcmv disease; group 2 ( n = 16) with <50/microl cd4(+) t-cell count and hcmv disease. all patients had previously undergone antiretroviral monotherapy or dual therapy before initiating haart. | 2001 | 11707667 |
| longitudinal observations on mutations conferring ganciclovir resistance in patients with acquired immunodeficiency syndrome and cytomegalovirus retinitis: the cytomegalovirus and viral resistance study group report number 8. | cytomegalovirus retinitis is the most common intraocular infection in patients with acquired immunodeficiency syndrome (aids). with prolonged suppressive anticytomegalovirus maintenance therapy, resistance occurs in over 25% of patients. we evaluated longitudinal changes in the cytomegalovirus genotype in patients with cytomegalovirus retinitis who developed ganciclovir resistance that was demonstrated in either the blood or urine. | 2001 | 11704031 |
| provision of laboratory services for heart and lung transplantation in australia. | laboratory services for the support of heart and lung transplantation in australia have adapted to the special needs of the clinicians looking after the heart and lung transplantation patients. | 2001 | 11694263 |
| methylene-gem-difluorocyclopropane analogues of nucleosides: synthesis, cyclopropene-methylenecyclopropane rearrangement, and biological activity. | alkylation-elimination of adenine and 2-amino-6-chloropurine with gem-difluorocyclopropane dibromide 10 gave e- and z-methylene-gem-difluorocyclopropanes 11a, 11b, 12a, and 12b and gem-difluorocyclopropenes 13a and 13b. debenzylation of intermediates 11a, 11b, 12a, and 12b afforded e- and z-methylenecyclopropanes 4a, 4b, 5a, and 5b. hydrolysis of 2-amino-6-chloropurine derivatives 4b and 5b afforded guanine analogues 4c and 5c. composition of products (except 14b) obtained from alkylation-elimin ... | 2001 | 11689090 |
| comparative analysis of human cytomegalovirus-specific cd4(+) t-cell frequency and lymphoproliferative response in human immunodeficiency virus-positive patients. | evaluation of human cytomegalovirus (hcmv)-specific t-helper immunity could contribute in optimizing anti-hcmv therapy in human immunodeficiency virus (hiv)-infected patients. testin the lymphoproliferative response (lpr) is the standard technique used to evaluate t-helper response, but its use in the routine diagnostic laboratory setting can be problematic. the most promising new alternative technique is the determination of hcmv-specific cd4(+) t-cell frequency by flow cytometry detection of i ... | 2001 | 11687467 |
| human cytomegalovirus strain toledo lacks a virus-encoded tropism factor required for infection of aortic endothelial cells. | human cytomegalovirus (hcmv) strains were investigated to identify those with altered tropism for endothelial cells. in viral replication kinetics analysis, hcmv strain toledo replicated poorly in aortic endothelial cells (aecs), and the virus count was reduced by 2-3 log units, in comparison with strain ad169. virus entry at the cell surface for each strain was equivalent. however, immunofluorescence studies revealed a lack of immediate early viral antigen 72 expression, and direct blot hybridi ... | 2001 | 11679913 |
| human cytomegalovirus productively infects porcine endothelial cells in vitro. | the possibility that human cytomegalovirus (hcmv) may infect porcine endothelial cells (ecs) was investigated. this may be relevant during xenotransplantation of porcine cells or organs into human recipients. | 2001 | 11602867 |
| cytomegalovirus pp65 antigen-guided preemptive therapy with ganciclovir in solid organ transplant recipients: a prospective, double-blind, placebo-controlled study. | the aim of this study was to evaluate pp65 antigen-guided antiviral therapy in preventing human cytomegalovirus (hcmv) infection in solid organ transplant recipients. | 2001 | 11602864 |
| human cytomegalovirus open reading frame trl11/irl11 encodes an immunoglobulin g fc-binding protein. | several herpesviruses encode fc receptors that may play a role in preventing antibody-mediated clearance of the virus in vivo. human cytomegalovirus (hcmv) induces an fc-binding activity in cells upon infection, but the gene that encodes this fc-binding protein has not been identified. here, we demonstrate that the hcmv ad169 open reading frame trl11 and its identical copy, irl11, encode a type i membrane glycoprotein that possesses igg fc-binding capabilities. | 2001 | 11602761 |
| differentiation-dependent redistribution of heparan sulfate in epithelial intestinal caco-2 cells leads to basolateral entry of cytomegalovirus. | human cytomegalovirus (hcmv) causes a broad spectrum of clinical manifestations in immunocompromised patients, including infection of the gastrointestinal tract. to investigate the role of epithelial cells in the gastrointestinal hcmv disease, we used the intestinal epithelial cell line caco-2, which is permissive for hcmv replication. in differentiated caco-2 cells, we showed previously that hcmv infection proceeds preferentially from the basolateral membrane, suggesting that receptors for hcmv ... | 2001 | 11601914 |
| identification of tnf-alpha-sensitive sites in hcmvie1 promoter. | viral vectors using the human cytomegalovirus immediate-early promoter (hcmvie1 promoter) are potentially efficient tools for gene delivery in vivo to diverse cell types. we previously demonstrated that two cytokines, tumor necrosis factor-alpha (tnf-alpha) and interferon-gamma (inf-gamma), inhibited transgene expression from this promoter in skeletal and cardiac myocytes. in this study, electrophoretic mobility shift assays (emsas) were performed to identify the tnf-alpha response elements from ... | 2001 | 11599916 |
| human cytomegalovirus gb genotype 1 is dominant in congenital infections in south hungary. | on the basis of the sequence variation of the glycoprotein b (gb) gene, human cytomegalovirus (hcmv) can be classified into four gb genotypes. genotyping of hcmv from congenital infections was carried out on the assumption that the envelope gb may influence the outcome of prenatal infection. sixty-three pregnant women were included in the study: 40 pregnant women whose fetuses were strongly suspected of having viral infection, and 23 women with normal pregnancies, from whom amniotic fluid was ta ... | 2001 | 11596091 |
| overexpression of the adenovirus type 12 (ad12) ptp or e1a gene facilitates ad12 dna replication in nonpermissive bhk21 hamster cells. | in the adenovirus type 12 (ad12) hamster cell system, abortive virus infection is one of the factors associated with the highly efficient oncogenesis in newborn syrian hamsters. we have shown earlier that the replication and efficient late transcription of the ad12 genome are blocked in syrian hamster cells. some of the early ad12 functions are transcribed in these cells, although at a minimal rate. in the present study, we demonstrate that low expression levels of the e1a and precursor to termi ... | 2001 | 11581373 |
| stable therapeutic serum levels of human alpha-1 antitrypsin (aat) after portal vein injection of recombinant adeno-associated virus (raav) vectors. | previous work from our group showed that recombinant adeno-associated virus (raav) vectors mediated long-term secretion of therapeutic serum levels of human alpha-1 antitrypsin (haat) after a single injection in murine muscle. we hypothesized that hepatocyte transduction could be even more efficient, since these cells represent the natural site of aat production and secretion. to test this hypothesis, raav vectors containing the haat cdna driven by either the human elongation factor 1 alpha prom ... | 2001 | 11571566 |
| up-regulation of fas ligand expression by human cytomegalovirus immediate-early gene product 2: a novel mechanism in cytomegalovirus-induced apoptosis in human retina. | human cmv (hcmv) is an important pathogen that causes widespread diseases in immunocompromised individuals. among the opportunistic hcmv infections, hcmv retinitis is most common in transplant recipients and aids patients. it often leads to blindness if left untreated. the question as to how hcmv infection causes retinal pathogenesis remains unresolved. here, we report that viral immediate-early gene product 2 (ie2), but not ie1, up-regulates the fas ligand (fasl) expression in hcmv-infected hum ... | 2001 | 11564832 |
| acyclic/carbocyclic guanosine analogues as anti-herpesvirus agents. | several guanosine analogues, i.e. acyclovir (and its oral prodrug valaciclovir), penciclovir (in its oral prodrug form, famciclovir) and ganciclovir, are widely used for the treatment of herpesvirus [i.e. herpes simplex virus type 1 (hsv-1), and type 2 (hsv-2), varicella-zoster virus (vzv) and/or human cytomegalovirus (hcmv)] infections. in recent years, several new guanosine analogues have been developed, including the 3-membered cyclopropylmethyl and -methenyl derivatives (a-5021 and synguanol ... | 2001 | 11563039 |
| human cytomegalovirus binding to heparan sulfate proteoglycans on the cell surface and/or entry stimulates the expression of human leukocyte antigen class i. | human cytomegalovirus (hcmv) is known to down-regulate the expression of human leukocyte antigen (hla) class i, the process of which involves a subset of virus genes. infection of human foreskin fibroblast (hff) cells with uv-inactivated hcmv (uv-hcmv), however, resulted in an increase in hla class i presentation on the cell surface in the absence of hcmv gene expression. heparin, which inhibits the interaction of virus particles with cell surface heparan sulfate proteoglycans (hspgs), blocked t ... | 2001 | 11562534 |
| activation of interferon response factor-3 in human cells infected with herpes simplex virus type 1 or human cytomegalovirus. | activation of cellular interferon-stimulated genes (isgs) after infection with herpes simplex virus type 1 (hsv-1) or human cytomegalovirus (hcmv) was investigated. the level of isg54-specific rna in human fetal lung (hfl) or human foreskin (bj) fibroblasts increased substantially after infection with either virus in the presence of cycloheximide. hsv-1 particles lacking glycoprotein d or glycoprotein h failed to induce isg54-specific rna synthesis, demonstrating that entry of virus particles ra ... | 2001 | 11533154 |
| ccaat/enhancer-binding proteins alpha and beta negatively influence the capacity of tumor necrosis factor alpha to up-regulate the human cytomegalovirus ie1/2 enhancer/promoter by nuclear factor kappab during monocyte differentiation. | recently we demonstrated that the ability of tumor necrosis factor alpha (tnfalpha) to stimulate the human cytomegalovirus (hcmv) ie1/2 enhancer/promoter activity in myeloid progenitor-like cells decreases when these cells differentiate into promonocytic cells. in addition, tnfalpha stimulation in the progenitor-like cell line hl-60 was shown to be mediated by nuclear factor kappab (nf-kappab) activation and its binding to the 18-base pair sequence motifs of the ie1/2 enhancer. we demonstrate he ... | 2001 | 11522776 |
| human cytomegalovirus a sequence and ul144 variability in strains from infected children. | human cytomegalovirus (hcmv) displays genetic polymorphisms. this variability may contribute to strain-specific tissue tropism and disease expression in hcmv-infected humans. to determine strain variability in a sequence and ul144 gene regions, 51 low-passage isolates from 44 hcmv-infected children were studied. isolates were obtained from 28 healthy children attending child care centers in iowa and from 16 congenitally infected infants born in texas. isolates demonstrated substantial nucleotide ... | 2001 | 11505449 |
| aciclovir selects for ganciclovir-cross-resistance of human cytomegalovirus in vitro that is only in part explained by known mutations in the ul97 protein. | phenotypically, ganciclovir-resistant human cytomegalovirus strains could be selected by aciclovir as effectively as by ganciclovir in vitro. three clinical human cytomegalovirus isolates with different sensitivities against ganciclovir, aciclovir, foscarnet, and cidofovir, but without any mutation in the viral ul97 protein known to confer ganciclovir resistance, were propagated each in duplicate in the presence of ganciclovir or aciclovir. after drug selection, all 12 strains were less suscepti ... | 2001 | 11505446 |
| characterization of the signal peptide processing and membrane association of human cytomegalovirus glycoprotein o. | human cytomegalovirus (hcmv) has a structurally complex envelope that contains multiple glycoproteins. these glycoproteins are involved in virus entry, virus maturation, and cell-cell spread of infection. glycoprotein h (gh), glycoprotein l (gl), and glycoprotein o (go) associate covalently to form a unique disulfide-bonded tripartite complex. glycoprotein o was recently discovered, and its basic structure, as well as that of the tripartite complex, remains uncharacterized. based on hydropathy a ... | 2001 | 11504733 |
| galpha(i2) enhances insulin signaling via suppression of protein-tyrosine phosphatase 1b. | suppression of the expression of the heterotrimeric g-protein galpha(i2) in vivo has been shown to provoke insulin resistance, whereas enhanced insulin signaling is observed when galpha(i2) is overexpressed in vivo. the basis for galpha(i2) regulation of insulin signaling was explored in transgenic mice with targeted expression of the gtpase-deficient, constitutively active q205l galpha(i2) in fat and skeletal muscle. phosphorylation of insulin receptor and irs-1 in response to insulin challenge ... | 2001 | 11500506 |
| technology evaluation: fomivirsen, isis pharmaceuticals inc/ciba vision. | fomivirsen (isis-2922, vitravene) is an antisense 21 mer phosphorothioate oligonucleotide with sequence complementarity to the coding region of the major immediate-early gene of human cytomegalovirus (cmv). developed by isis pharmaceuticals inc, fomivirsen is the first antisense oligonucleotide to receive approval for licensing and is marketed by novartis' ciba vision. fomivirsen is administered by intravitreal injection to aids patients for the treatment of cmv-induced retinitis. in august 1998 ... | 2001 | 11497353 |
| human cytomegalovirus circumvents nf-kappa b dependence in retinal pigment epithelial cells. | the human cmv (hcmv) is a persistent virus that may cause severe inflammatory responses especially in immunocompromised hosts. in different cell types, hcmv infection leads to the activation of the pleiotropic transcription factor, nf-kappab, which triggers virus replication but also propagates cell-mediated inflammatory mechanisms that largely depend on pg synthesis. we investigated the interactions of hcmv and the nf-kappab-dependent pg synthesis pathway in cultures of retinal pigment epitheli ... | 2001 | 11489969 |
| scleral plug of biodegradable polymers containing ganciclovir for experimental cytomegalovirus retinitis. | to evaluate the efficacy of a biodegradable scleral plug containing ganciclovir (gcv) in a rabbit model of human cytomegalovirus (hcmv) retinitis. | 2001 | 11481270 |
| complete replication of human cytomegalovirus in explants of first trimester human placenta. | tissue integrity and viability of first trimester placenta explants were obtained in culture for 3 weeks. explants were infected with human cytomegalovirus (hcmv), several cycles of hcmv replication were obtained and the progression of the infection was observed within a tissue that maintains its normal cellular organization. in agreement with recent clinical data, 3 weeks were necessary for the virus to colonize the placenta fully. complete hcmv replication was observed in trophoblasts, followe ... | 2001 | 11468735 |
| reactivation of latent human cytomegalovirus in cd14(+) monocytes is differentiation dependent. | we have previously demonstrated reactivation of latent human cytomegalovirus (hcmv) in myeloid lineage cells obtained from healthy donors. virus was obtained from allogenically stimulated monocyte-derived macrophages (allo-mdm), but not from macrophages differentiated by mitogenic stimulation (cona-mdm). in the present study, the cellular and cytokine components essential for hcmv replication and reactivation were examined in allo-mdm. the importance of both cd4(+) and cd8(+) t cells in the gene ... | 2001 | 11462026 |
| protection of chickens from lethal avian influenza a virus infection by live-virus vaccination with infectious laryngotracheitis virus recombinants expressing the hemagglutinin (h5) gene. | the h5 hemagglutinin (ha) gene of a highly pathogenic avian influenza virus (aiv) isolate (a/chicken/italy/8/98) was cloned and sequenced, and inserted at the non-essential ul50 (dutpase) gene locus of a virulent strain of infectious laryngotracheitis virus (iltv). northern and western blot analyses of the obtained iltv recombinants demonstrated stable expression of the ha gene under control of the human cytomegalovirus immediate-early gene promoter. in vitro replication of the ha-expressing ilt ... | 2001 | 11457552 |
| human cytomegalovirus infection: diagnostic potential of recombinant antigens for cytomegalovirus antibody detection. | recombinant antigen-based enzyme immunoassays (eias) for the detection of human cytomegalovirus (hcmv) specific antibody are believed to yield a higher sensitivity and specificity than virus lysate eias. the aim of the present study was to evaluate the accuracy of newly established hcmv assays (copalis cmv multiplex, sorin; cobas core cmv igg and igm eias, roche diagnostics; anti-hcmv recombinant igg, gb-igg, igm and iga, biotest; and eti-cytok-g plus and m reverse plus, sorin) based on recombin ... | 2001 | 11445146 |
| human immunodeficiency virus-infected patients receiving highly active antiretroviral therapy maintain activated cd8+ t cell subsets as a strong adaptive immune response to cytomegalovirus. | cd8(+) t lymphocyte function specific for human cytomegalovirus (cmv) was evaluated in 14 patients infected with human immunodeficiency virus (hiv) receiving highly active antiretroviral therapy (haart) and 26 cmv-seropositive donors without hiv infection. fifty-seven percent of the hiv-infected group had cmv-specific cytolytic activity in freshly isolated peripheral blood mononuclear cells (pbmc) against targets expressing cmv pp65. both interferon (ifn)-gamma secretion by cd8(+) t cells and th ... | 2001 | 11443550 |
| in vitro and in vivo activity of 1-o-hexadecylpropanediol-3-phospho-ganciclovir and 1-o-hexadecylpropanediol-3-phospho-penciclovir in cytomegalovirus and herpes simplex virus infections. | human cytomegalovirus (hcmv) and herpes simplex virus (hsv) can cause a wide variety of clinical manifestations in man. ganciclovir (gcv) is effective against hcmv infection when administered by the intravenous route and may be used orally in large doses for prophylaxis of hcmv infections in organ transplantation patients and in aids patients. in previous studies with acyclovir (acv), we found that covalent attachment of an alkyl glycerol phosphate moiety greatly increased oral bioavailability a ... | 2001 | 11437323 |
| antiviral drug resistance in human cytomegalovirus. | drug-resistant cytomegalovirus (cmv) should be considered when viral shedding persists after several weeks of therapy. the problem is most likely to arise in the setting of a severely immunosuppressed host with continuing or relapsing disease. not all treatment failure can be attributed to drug resistance. the testing of cmv isolates for drug resistance in cell culture is time-consuming and labor-intensive, but recent advances in understanding of the genetics of resistance have resulted in rapid ... | 1999 | 11428978 |
| inhibition of cytomegalovirus immediate early gene expression: a therapeutic option? | the replication cycle of the human cytomegalovirus (hcmv) is characterized by the expression of immediate early (ie), early (e), and late (l) gene regions. current antiviral strategies are directed against the viral dna polymerase expressed during the early phase of infection. the regulation of the ie-1 and ie-2 gene expression is the key to latency and active replication due to their transactivating and repressing functions. there is growing evidence that the pathogenic features of hcmv are lar ... | 2001 | 11428240 |
| viral load in breast milk correlates with transmission of human cytomegalovirus to preterm neonates, but lactoferrin concentrations do not. | in vitro, lactoferrin (lf) strongly inhibits human cytomegalovirus (hcmv), which led us to hypothesize that in vivo hcmv might also be inhibited in secretions with high lf concentrations. in breast milk, high viral loads observed as high viral dna titers tended to coincide with higher lf levels. however, the lf levels did not correlate to virus transmission to preterm infants. the viral load in the transmitting group was highest compared to the nontransmitting group. we conclude that viral load ... | 2001 | 11427433 |
| cross-reactivity of epstein-barr virus-specific immunoglobulin m antibodies with cytomegalovirus antigens containing glycine homopolymers. | timely and reliable detection of acute primary human cytomegalovirus (hcmv) infection is important in prenatal screening programs and for differential diagnosis of infectious mononucleosis-like disease. enzyme-linked immunosorbent assays (elisas) based on hcmv proteins enable the sensitive detection of immunoglobulin m (igm) antibodies during primary infection. however, concerns have been raised about possible cross-reactivities of the hcmv antigens used for the design of such elisas with igm an ... | 2001 | 11427421 |
| optimization of dna immunization against human cytomegalovirus. | the immune responses of mice injected with plasmids vr-gb and vr-gb delta tm expressing the full-length membrane-anchored, or secreted forms of human cytomegalovirus (hcmv)-glycoprotein b (gb), respectively, and vr-pp65 expressing the hcmv-phosphoprotein 65 (pp65) were analyzed. pretreatment of mice with the local anesthetic bupivacaine did not enhance antibody production, and ifn-alpha co-expressed with the immunizing plasmids induced a moderate increase in the antibody response. however, antib ... | 2001 | 11427273 |
| replication of human cytomegalovirus in severe combined immunodeficient mice implanted with human retinal tissue. | because human cytomegalovirus (hcmv) infection and replication are limited to human cells, few animal models can be used to specifically examine the biology of hcmv in vivo. in these studies, fetal human retinal tissue was implanted into the anterior chamber of the severe combined immunodeficient (scid) mouse eye and subsequently was inoculated with hcmv. viral replication, localized to glial cells in the xenografts, was first detected 7 days after infection. thereafter, hcmv replication increas ... | 2001 | 11424017 |
| detection of human cytomegalovirus (hcmv) pp67-mrna and pp65 antigenemia in relation to development of clinical hcmv disease in renal transplant recipients. | to evaluate the performance of the recently introduced method based on detection of human cytomegalovirus (hcmv) pp67 mrna in blood by the nucleic acid sequence-based amplification (nuclisens), in comparison to semiquantitative detection of pp65 hcmv antigen in white blood cells, in relation to development of clinical hcmv disease. | 2001 | 11422252 |
| the carboxyl terminus of the human cytomegalovirus ul37 immediate-early glycoprotein is conserved in primary strains and is important for transactivation. | the human cytomegalovirus (hcmv) ul37 exon 3 (ul37x3) open reading frame (orf) encodes the carboxyl termini of two immediate-early glycoproteins (gpul37 and gpul37(m)). ul37x3 homologous sequences are not required for mouse cytomegalovirus (mcmv) growth in vitro; yet, they are important for mcmv growth and pathogenesis in vivo. similarly, ul37x3 sequences are dispensable for hcmv growth in culture, but their requirement for hcmv growth in vivo is not known. to determine this requirement, we dire ... | 2001 | 11413367 |
| experimental preemptive immunotherapy of murine cytomegalovirus disease with cd8 t-cell lines specific for ppm83 and pm84, the two homologs of human cytomegalovirus tegument protein ppul83 (pp65). | cd8 t cells are the principal antiviral effectors controlling cytomegalovirus (cmv) infection. for human cmv, the virion tegument protein ppul83 (pp65) has been identified as a source of immunodominant peptides and is regarded as a candidate for cytoimmunotherapy and vaccination. two sequence homologs of ppul83 are known for murine cmv, namely the virion protein ppm83 (pp105) expressed late in the viral replication cycle and the nonstructural protein pm84 (p65) expressed in the early phase. here ... | 2001 | 11413326 |
| the human cytomegalovirus us28 protein is located in endocytic vesicles and undergoes constitutive endocytosis and recycling. | genes encoding chemokine receptor-like proteins have been found in herpes and poxviruses and implicated in viral pathogenesis. here we describe the cellular distribution and trafficking of a human cytomegalovirus (hcmv) chemokine receptor encoded by the us28 gene, after transient and stable expression in transfected hela and cos cells. immunofluorescence staining indicated that this viral protein accumulated intracellularly in vesicular structures in the perinuclear region of the cell and showed ... | 2001 | 11408581 |
| generation of cytotoxic t lymphocytes specific for human cytomegalovirus using dendritic cells in vitro. | summary: for the adoptive immunotherapy in immunodeficient bone marrow transplant recipients to prevent and treat human cytomegalovirus (hcmv)-associated diseases, hcmv-pulsed dendritic cells (dcs) were used as antigen-presenting cells for the induction of cytotoxic t lymphocytes (ctls) specific to hcmv antigens in vitro. the antiviral ctl responses induced by hcmv-pulsed dcs were as highly efficient as those induced by hcmv-infected dermal fibroblasts, and endogenous viral gene expression was n ... | 2001 | 11395640 |
| [dynamic of immune response in primary cytomegalovirus infection and after reactivation of cytomegalovirus in patients with organ allotransplantation]. | a total of 44 serum specimens from 7 patients with kidneys or liver transplanted from donors who had antibodies (ab) to human cytomegalovirus (cmv) were studied. in 4 recipients anti-cmv ab were found before transplantation and in 3 others they were not detected. it was shown by eia that igm and igg anti-cmv appeared in the sera of primarily infected patients after 1-2 weeks and their titers were 5-10 times lower than in patients with reactivated cmv infection. immunoblotting of ab to individual ... | 2001 | 11392966 |
| global modulation of cellular transcription by human cytomegalovirus is initiated by viral glycoprotein b. | human cytomegalovirus (hcmv) infection alters the expression of many cellular genes, including ifn-stimulated genes (isgs) [zhu, h., cong, j.-p., mamtora, g., gingeras, t. & shenk, t. (1998) proc. natl. acad. sci. usa 95, 14470-14475]. by using high-density cdna microarrays, we show that the hcmv-regulated gene expression profile in fibroblasts does not differ substantially from the response generated by ifn. furthermore, we identified the specific viral component triggering this response as the ... | 2001 | 11390970 |
| human cytomegalovirus chemokine receptor gene us28 is transcribed in latently infected thp-1 monocytes. | the human cytomegalovirus (hcmv) us28 gene product, pus28, is a g protein-coupled receptor that interacts with both cc and cx(3)c chemokines. to date, the role of pus28 in immune evasion and cell migration has been studied only in cell types that can establish productive hcmv infection. we show that hcmv can latently infect thp-1 monocytes and that during latency us28 is transcribed. we also show that the transcription is sustained during differentiation of the thp-1 monocytes. since cells expre ... | 2001 | 11390596 |
| cmv infection of liver transplant recipients: comparison of antigenemia and molecular biology assays. | cmv is a major clinical problem in transplant recipients. thus, it is important to use sensitive and specific diagnostic techniques to rapidly and accurately detect cmv infection and identify patients at risk of developing cmv disease. in the present study, cmv infection after liver transplantation was monitored retrospectively by two molecular biology assays - a quantitative pcr assay and a qualitative nasba assay. the results were compared with those obtained by prospective pp65 antigenemia de ... | 2001 | 11389774 |
| epstein-barr virus-encoded protein kinase bglf4 mediates hyperphosphorylation of cellular elongation factor 1delta (ef-1delta): ef-1delta is universally modified by conserved protein kinases of herpesviruses in mammalian cells. | translation elongation factor 1delta (ef-1delta) is hyperphosphorylated in various mammalian cells infected with alpha-, beta- and gammaherpesviruses and ef-1delta modification is mediated by viral protein kinases, including ul13 of herpes simplex virus type 1 and ul97 of human cytomegalovirus. in this study, the following is reported. (i) bglf4 encoded by the prototype gammaherpesvirus epstein-barr virus was purified as a fusion protein that was labelled with [gamma-(32)p]atp and labelling was ... | 2001 | 11369891 |
| inhibitors of human cytomegalovirus replication drastically reduce the activity of the viral protein kinase pul97. | the ul97-encoded protein kinase (pul97) of human cytomegalovirus (hcmv) plays a critical role in the control of virus replication. deletion of the ul97 gene results in a drastic reduction in the replication efficiency. although the exact function of pul97 remains unclear and its sensitivity to specific inhibitors is speculative, protein kinase inhibitors of the indolocarbazole class are effective inhibitors of cytomegalovirus. based on the phosphorylation of ganciclovir (gcv), a novel quantifica ... | 2001 | 11369889 |
| in vitro selection of human cytomegalovirus variants unable to transfer virus and virus products from infected cells to polymorphonuclear leukocytes and to grow in endothelial cells. | four human cytomegalovirus (hcmv) isolates from different clinical sources were extensively propagated in human embryonic lung fibroblasts (helf). plaque isolates from each of the four virus strains were evaluated for their ability to be transferred to polymorphonuclear leukocytes (pmnl) and to grow in endothelial cells (ec). while all four of the clinical strains were found to be both pmnl- and ec-tropic, variants were identified from each of the four strains that lacked both biological propert ... | 2001 | 11369888 |
| [diagnostic value of human cytomegalovirus late-mrna detection for intrauterine active human cytomegalovirus infection]. | to study diagnostic value of late-mrna detection for intrauterine active human cytomegalovirus (hcmv) infection. | 1999 | 11360611 |
| detection and quantification of human cytomegalovirus (cmv) as a marker for development of cmv disease and survival in patients with aids. | 1997 | 11327439 | |
| susceptibility of human cytomegalovirus to two-drug combinations in vitro. | human cytomegalovirus (hcmv) is a major cause of morbidity and mortality for immunocompromised hosts. we sought to determine the in vitro susceptibility of hcmv reference laboratory strains, clinical isolates and strains with known resistance to currently available anticytomegaloviral drugs to two-drug combinations of the following compounds: ganciclovir, foscarnet, cidofovir and its cyclic congener, cyclic hpmpc (chpmpc), and lobucavir. cytotoxicity was determined by trypan blue exclusion of ce ... | 1996 | 11324826 |
| analysis and characterization of the complete genome of tupaia (tree shrew) herpesvirus. | the tupaia herpesvirus (thv) was isolated from spontaneously degenerating tissue cultures of malignant lymphoma, lung, and spleen cell cultures of tree shrews (tupaia spp.). the determination of the complete nucleotide sequence of the thv strain 2 genome resulted in a 195,857-bp-long, linear dna molecule with a g+c content of 66.5%. the terminal regions of the thv genome and the loci of conserved viral genes were found to be g+c richer. furthermore, no large repetitive dna sequences could be ide ... | 2001 | 11312357 |
| sorting of glycoprotein b from human cytomegalovirus to protein storage vesicles in seeds of transgenic tobacco. | as part of ongoing studies into the use of plant expression systems for making human therapeutic proteins, we have successfully expressed the major glycoprotein, gb, of human cytomegalovirus (hcmv) in transgenic tobacco plants. viral glycoprotein was detectable in the protein extracts of mature tobacco seeds using neutralizing and non-neutralizing monoclonal antibodies specific for gb. although several mammalian proteins have been expressed in tobacco, localization of these proteins in transgeni ... | 2001 | 11305363 |
| immune evasion by human cytomegalovirus: lessons in immunology and cell biology. | the human cytomegalovirus (hcmv) has dedicated a significant part of its genome to genes encoding molecules that modulate the host immune response. many of these genes have homologues in the host genome. others, however, are unique in the sense that no obvious primary sequence identity is found in the available databases. the hcmv gene products interfere with the activation of mhc class i and class ii restricted t cells and nk cells, modify the function of cytokines and their receptors, interact ... | 2001 | 11289798 |
| degradation of p21cip1 in cells productively infected with human cytomegalovirus. | human cytomegalovirus (hcmv) stimulates arrested cells to enter the cell cycle by activating cyclin-dependent kinases (cdks), notably cdk2. several mechanisms are involved in the activation of cdk2. hcmv causes a substantial increase in the abundance of cyclin e and stimulates translocation of cdk2 from the cytoplasm to the nucleus. further, the abundance of the cdk inhibitors (ckis) p21cip1/waf1 (p21cip1) and p27kip1 is substantially reduced. the activity of cyclin e/cdk2 increases as levels of ... | 2001 | 11264351 |
| evaluation of five commercial enzyme immunoassays for the detection of human cytomegalovirus-specific igm antibodies in the absence of a commercially available gold standard. | in the recent years the number of commercially available immunoassays for the detection of human cytomegalovirus (hcmv)-specific immunoglobulin m (igm) antibodies has rapidly increased. the aim of the present study was to evaluate five commercial immunoassays for the serological diagnosis of hcmv-infection. these methods, namely the imx cmv igm assay, the axsym cmv igm assay (both abbott), the gull cmv igm, the cmv-igm-ela test pcs medac and the biotest anti-hcmv recombinant igm elisa, were comp ... | 2001 | 11256803 |