Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| immunological cross reactivity of eaea (intimin) from e. coli that cause attaching and effacing lesions in humans and rabbits. | 1997 | 9192001 | |
| a putative monofunctional glycosyltransferase is expressed in ralstonia eutropha. | a gene, mgt, encoding a protein homologous to the n-terminal module of class a high-molecular-mass penicillin-binding proteins was identified in ralstonia eutropha. by using specific antibodies, the corresponding mgt protein was detected in association with the membrane, confirming that the n-terminal hydrophobic segment functioned as a membrane anchor. a derivative in which the hydrophobic sequence was deleted was overexpressed as a maltose-binding fusion protein in escherichia coli. cleavage o ... | 1997 | 9190828 |
| localization of the in vivo expression of p and f1 fimbriae in chickens experimentally inoculated with pathogenic escherichia coli. | escherichia coli causing septicemia in poultry often possess f1 (type 1) and/or p fimbriae which may be involved in bacterial colonization and infection. to investigate the expression of these fimbriae in vivo, two pathogenic e. coli strains with different fimbrial profiles, tk3 (fim+/pap+) and mt78 (fim+/pap-), were administered to 2-week-old chickens by either the intratracheal or caudal thoracic air sac inoculation route. antibodies specific for native f1 fimbriae were detected by elisa and i ... | 1997 | 9188088 |
| release of replication-deficient retroviruses from a packaging cell line: interaction with glioma tumor spheroids in vitro. | the present study describes how various growth conditions affect gene expression and virus production from a retroviral packaging cell line (liz 9), grown as monolayers and as multicellular spheroids. in addition, to study the direct interaction between packaging cells and tumor tissue of glioma origin, liz 9 spheroids were confronted with tumor spheroids derived from a human glioma cell line, gamg. the results show a progressive gene transfer into the tumor tissue, with 9% transfection efficacy ... | 1997 | 9180159 |
| structural basis for differential receptor binding of cholera and escherichia coli heat-labile toxins: influence of heterologous amino acid substitutions in the cholera b-subunit. | the closely related b-subunits of cholera toxin (ctb) and escherichia coli heat-labile enterotoxin (ltb) both bind strongly to gm1 ganglioside receptors but ltb can also bind to additional glycolipids and glycoproteins. a number of mutant ct b-subunits were generated by substituting ctb amino acids with those at the corresponding positions in ltb. these were used to investigate the influence of specific residues on receptor-binding specificity. a mutated ctb protein containing the first 25 resid ... | 1997 | 9179843 |
| cdna cloning, expression, and rapid purification of a kunitz-type winged bean chymotrypsin inhibitor. | a 183-residue kunitz-type winged bean chymotrypsin inhibitor (wbci), inhibits its cognate protease at a molar ratio of 1:2, instead of the usual ratio of 1:1 common to other members of the family. from the cdna pool obtained by reverse transcription of the poly(a)+ rna of the developing winged bean seeds, the structural gene of wbci has been amplified by pcr using primers designed to delete the 24-residue signal peptide and introduce ecori and sali sites at the ends of the amplified dna. the lat ... | 1997 | 9179296 |
| prelytic and lytic conformations of erythrocyte-associated escherichia coli hemolysin. | flow cytometry was developed as a method to assess the conformation of erythrocyte-bound escherichia coli hemolysin polypeptide (hlya). topology of membrane-associated hemolysin (hlya(e)) was investigated by testing surface accessibility of hlya regions in lytic and nonlytic bound states, using a panel of 12 anti-hlya monoclonal antibodies (mabs). hemolysin associates nonlytically with erythrocytes at 0 to 2 degrees c. to test the hypothesis that the nonlytic hlya(e) conformation at 0 to 2 degre ... | 1997 | 9169756 |
| a third secreted protein that is encoded by the enteropathogenic escherichia coli pathogenicity island is required for transduction of signals and for attaching and effacing activities in host cells. | enteropathogenic escherichia coli strains are able to signal host cells, cause dramatic cytoskeletal rearrangements, and adhere intimately to the cell surface in a process known as the attaching and effacing effect. a pathogenicity island of 35 kb known as the locus of enterocyte effacement (lee) is necessary and sufficient for this effect. the lee encodes an outer membrane adhesin called intimin, a type iii secretion apparatus, and the espa and espb secreted proteins. the dna sequence of the re ... | 1997 | 9169753 |
| delivery of primary autologous skeletal myoblasts into rabbit heart by coronary infusion: a potential approach to myocardial repair. | myocardial repair after injury is limited because the adult heart cannot regenerate. we propose using autologous skeletal muscle cells (myoblasts) as a source of reserve cells for repair of regions of damaged myocardium. this report examines two potential methods for the transfer of cells to the myocardium: selective coronary catheterization, and myoblast infusion or myoblast injection directly into the left ventricular wall. autologous, primary rabbit skeletal myoblasts were harvested, were tra ... | 1997 | 9154641 |
| accessory subunit of mitochondrial dna polymerase from drosophila embryos. cloning, molecular analysis, and association in the native enzyme. | a full-length cdna of the accessory (beta) subunit of mitochondrial dna polymerase from drosophila embryos has been obtained, and its nucleotide sequence was determined. the cdna clone encodes a polypeptide with a deduced amino acid sequence of 361 residues and a predicted molecular mass of 41 kda. the gene encoding the beta subunit lies within 4 kilobase pairs of that for the catalytic subunit in the drosophila genome, on the left arm of chromosome 2. the two genes have similar structural featu ... | 1997 | 9153213 |
| characterization of a large transferrin-binding protein from actinobacillus pleuropneumoniae serotype 7. | the binding of transferrin at the surface of actinobacillus pleuropneumoniae (a. pp.) is mediated by two proteins of approximately 60 and 100 kda. the 60 kda protein has been shown to be highly divergent among different serotypes and to induce a serotype-specific protective immune response. in this study we have characterized the 100 kda transferrin-binding protein of a. pp. serotype 7 and designated it as tfbb. the tfbb gene was found to be located immediately downstream of the tfba gene. it wa ... | 1997 | 9151535 |
| efficient autoproteolytic processing of the mhv-a59 3c-like proteinase from the flanking hydrophobic domains requires membranes. | the replicase gene of the coronavirus mhv-a59 encodes a serine-like proteinase similar to the 3c proteinases of picornaviruses. this proteinase domain is flanked on both sides by hydrophobic, potentially membrane-spanning, regions. cell-free expression of a plasmid encoding only the 3c-like proteinase (3clpro) resulted in the synthesis of a 29-kda protein that was specifically recognized by an antibody directed against the carboxy-terminal region of the proteinase. a protein of identical mobilit ... | 1997 | 9143287 |
| ono-5046, an elastase inhibitor, attenuates endotoxin-induced acute lung injury in rabbits. | endotoxin causes acute lung injury resembling acute respiratory distress syndrome. elastase, as well as reactive oxygen species released from activated neutrophils, are thought to play pivotal roles in the pathogenesis of this lung injury. this study investigated whether ono-5046, a specific elastase inhibitor, can attenuate acute lung injury induced by endotoxin in rabbits. thirty-two male anesthetized rabbits were randomly assigned to receive one of four treatments (n = 8 for each group): infu ... | 1997 | 9141938 |
| antibody reactivities to tumor-suppressor protein p53 and htlv-i tof, rex and tax in htlv-i-infected people with differing clinical status. | since the presence of anti-p53 antibody has been correlated with the mutation and accumulation of p53, the aim of this study was to detect anti-p53 antibody and understand its correlations with anti-tof, -rex, or -tax antibody reactivity in htlv-i infected people differing in their clinical status. a plasmid (pgex-tof) was constructed to express tof recombinant protein (rp) in escherichia coli. serum samples from 50 asymptomatic carriers (acs), 50 adult t-cell leukemia (atl) and 50 htlv-i-associ ... | 1997 | 9139842 |
| analysis of epitope structure of psp94 (prostate secretory protein of 94 amino acids): (i). immuno-dominant and immuno-recessive area. | psp94 is a potential biomarker for evaluating patients with prostate carcinoma. we have systematically studied the epitope structure of psp94 by using a polyclonal antibody against human psp94. results of peptide mapping and elisa tests of dose response to rabbit antiserum against human psp94 protein showed that only the n-terminal peptides (n30 and m23) are immunoreactive while all the synthetic peptides (c28, c10) located closer to the c-terminus are completely devoid of antigenic activity wit ... | 1997 | 9136076 |
| effects of low power laser-irradiation on differential blood count and body temperature in endotoxin-preimmunized rabbits. | low power laser irradiation has been shown to have various immune-modulatory effects under in vitro conditions but little is known about such effects in animal models. escherichia coli endotoxin-preimmunized rabbits were used to determine the influence of transcutaneously applied low power laser light on differential blood count and rectal temperature. after three initial immunizations animals were either boostered with 5 ng/kg of endotoxin or injected with pyrogen-free saline and subsequently u ... | 1997 | 9129122 |
| molecular cloning and expression of subunit 9 of the 26s proteasome. | seven peptides from subunit 9 (s9) of the human 26s proteasome were sequenced and this information was used to clone a hela cdna that encodes the 46 kda subunit. rabbit polyclonal antisera were made against a ubiquitin fusion protein containing 12 amino acids from s9 and against a full-length s9 expressed in e. coli. western blot analysis showed that the s9-specific antibodies bound the 26s proteasome and its regulatory complex separated on non-denaturing gels. in sds-page samples of the two com ... | 1997 | 9119060 |
| molecular, biological, and preliminary structural analysis of recombinant bryodin 1, a ribosome-inactivating protein from the plant bryonia dioica. | bryonia dioica (cucurbitaceae family) produces at least two type i ribosome-inactivating proteins, bryodin 1 (bd1) and bryodin 2 (bd2). a cdna sequence encoding bd1 was isolated from b. dioica leaf mrna using degenerative oligonucleotides and codes for a 22 amino acid signal peptide followed by a protein of 267 residues. expression of two recombinant bd1 (rbd1) forms in escherichia coli yielded proteins of 267 (to the natural stop codon) and 247 amino acids (to the putative cleavage site yieldin ... | 1997 | 9115985 |
| characterization of p0, a ribosomal phosphoprotein of plasmodium falciparum. antibody against amino-terminal domain inhibits parasite growth. | a cdna expression clone of the human malarial parasite plasmodium falciparum, lambdapf4, which was reactive only to the immune sera and not to the patient sera, has recently been found to be the p. falciparum homologue of the p0 ribosomal phosphoprotein gene. a northern analysis of the p0 gene revealed the presence of two transcripts, both present in all the different intraerythrocytic stages of the parasite life cycle. a 138-base pair amino-terminal domain of this gene was expressed as a fusion ... | 1997 | 9115284 |
| prevention of mucosal escherichia coli infection by fimh-adhesin-based systemic vaccination. | virtually all uropathogenic strains of escherichia coli, the primary cause of cystitis, assemble adhesive surface organelles called type 1 pili that contain the fimh adhesin. sera from animals vaccinated with candidate fimh vaccines inhibited uropathogenic e. coli from binding to human bladder cells in vitro. immunization with fimh reduced in vivo colonization of the bladder mucosa by more than 99 percent in a murine cystitis model, and immunoglobulin g to fimh was detected in urinary samples fr ... | 1997 | 9110982 |
| a function for the qkraa amino acid motif: mediating binding of dnaj to dnak. implications for the association of rheumatoid arthritis with hla-dr4. | the amino acid motif qkraa, when expressed on hla-drb1, carries susceptibility to develop rheumatoid arthritis. this motif is the basis of strong b and t cell epitopes. furthermore, it is highly overrepresented in protein databases, suggesting that it carries a function of its own. to identify this function, we used qkraa peptide affinity columns to screen total protein extracts from escherichia coli. we found that dnak, the e. coli 70-kd heat shock protein, binds qkraa. of interest, dnak has a ... | 1997 | 9109425 |
| progress towards using recombinant myxoma virus as a vector for fertility control in rabbits. | the history of myxoma virus, its use in australia as a mortality agent and the development of the virus as a vector for controlling fertility in wild rabbit populations in australia is reviewed. myxoma virus recombinants have been constructed to express model antigens. four potential insertion sites in the genome have been identified and two have been used to construct single and double recombinant viruses expressing escherichia coli enzymes beta-galactosidase and beta-glucuronidase. another rec ... | 1997 | 9109196 |
| oxa1p, which is required for cytochrome c oxidase and atp synthase complex formation, is embedded in the mitochondrial inner membrane. | we have previously isolated the yeast nuclear gene oxa1 and showed that oxa1p is required for the formation of the cytochrome c oxidase and atp synthase complexes. we have expressed oxa1p in e. coli and shown that it is toxic and rapidly degraded. nevertheless, a truncated protein was successfully expressed and antibodies have been raised against this truncated protein. these antibodies recognise a protein in mitochondrially enriched fractions. in vitro mitochondrial import experiments demonstra ... | 1997 | 9108137 |
| plant immunomodulators for termination of unwanted pregnancy and for contraception and reproductive health. | neem (azadirachta indica) seed and leaf extracts have spermicidal, anti-microbial, anti-fungal and anti-viral properties. they are also immunomodulators that induce primarily a th1 type response. these properties are being exploited to develop two different useful methods of fertility control. neem extracts given orally at early post-implantation stage terminate pregnancy in rodents and primates. treatment has no residual permanent effect and fertility is regained in subsequent cycles. the mecha ... | 1997 | 9107574 |
| ca2+-dependent membrane bound protein fraction from rabbit gastric mucosa contains a protein whose histidyl residue is phosphorylated. | we found an autophosphorylated protein with a molecular weight of 40 kda (p40) in the crude annexin fraction of rabbit gastric mucosa, i.e., the materials released by egta from the membrane fraction obtained in the presence of ca2+. this protein was enriched in chief cells in the gastric glands, and also found in the heart and the liver by western blotting. the protein bound to phenyl-sepharose in the presence of ca2+ and showed extremely basic nature. the phosphorylation site of p40 was conside ... | 1997 | 9099993 |
| studies by site-directed mutagenesis of the carbohydrate-binding properties of a bark lectin from robinia pseudoacacia. | a bark lectin, rbl, from robinia pseudoacacia (black locust), binds galactose-related sugars specifically. recombinant rbl (rrbl) with a histidine tag was expressed in escherichia coli, purified and characterized. rrbl agglutinated rabbit erythrocytes and the hemagglutination was inhibited by galactose and related sugars. to elucidate the mechanism of the binding of carbohydrate by rbl, 16 mutant rrbls were produced by site-directed mutagenesis. the analysis of the mutants indicated that residue ... | 1997 | 9091320 |
| prevalence and characteristics of enteropathogenic escherichia coli with the eae gene in diarrhoeic rabbits. | a field study was carried out with the objective of investigating the prevalence of enteropathogenic escherichia coli (epec) with the eae gene in diarrhoeic rabbits. epec eae+ were isolated from 60 (74%) of 81 diarrhoeic rabbits sampled in 30 industrial fattening farms localized in the four provinces of galicia (northwestern spain). attaching and effacing lesions were found in 44 of 50 animals processed for histology. the 111 e. coli strains identified belonged to 19 different o serogroups and 1 ... | 1997 | 9087949 |
| role of glutamine in bacterial transcytosis and epithelial cell injury. | l-glutamine is the principal energy source for small intestinal enterocytes. diminution of intestinal function, mucosal atrophy, and increased bacterial translocation have been noted during total parenteral nutrition (tpn). in a rat model of glutamine starvation, we previously showed that luminal glutamine is essential for optimal intestinal function. in this study, we examined the effect of apical vs basolateral glutamine on bacterial translocation in a caco-2 cell culture system and bacteria-i ... | 1997 | 9084009 |
| large-scale preparation and characterization of recombinant ovine placental lactogen. | to clone ovine placental lactogen (opl) cdna, total rna from sheep placental cotyledon was reverse transcribed and the single-stranded cdna was pcr-amplified with 5' and 3' primers containing, respectively, ncoi and psti sites. the opl cdna fragment amplified between these two primers extended from a(-1) to the natural stop codon. the pcr product was gel-purified and subcloned into a puc vector and the insert was sequenced on both strands, revealing several differences relative to the published ... | 1997 | 9071989 |
| synthesis of 4- and 5-series leukotrienes in the lung microvasculature challenged with escherichia coli hemolysin: critical dependence on exogenous free fatty acid supply. | escherichia coli hemolysin (hlya) has been identified as a potent inductor of phosphoinositide hydrolysis and related metabolic responses in neutrophils (grimminger and colleagues, 1991, j. clin. invest. 88:1531-1539). in isolated perfused rabbit lungs, which harbor a large number of entrapped microvascular leukocytes, we investigated the effect of a low dose of hlya on lipoxygenase product formation in the presence of exogenous free arachidonic acid (aa), eicosapentaenoic acid (epa), or both pr ... | 1997 | 9070617 |
| development of polyclonal antibodies for detection of aflatoxigenic molds involving culture filtrate and chimeric proteins expressed in escherichia coli. | polyclonal antibodies (pab) were raised against an aflatoxigenic strain of aspergillus parasiticus by using two different sources for antibody elicitation: (i) filtrate of a culture on which the fungus had been grown (ii) and two chimeric proteins, expressed in escherichia coli as separate products, of the genes ver-1 and apa-2, which are involved in aflatoxin biosynthesis. the gene products were amplified by pcr, and each was cloned into the e. coli expression vector pgex2t. upon induction, the ... | 1997 | 9055416 |
| rice ragged stunt oryzavirus genome segment 9 encodes a 38 600 mr structural protein. | the complete nucleotide sequence of rice ragged stunt virus genome segment 9 (s9) was determined. the s9 segment is 1132 nucleotides long and has a long open reading frame starting from the first aug codon at nucleotide position 14-16 and terminating at a uag codon located at 1028-1030, which could encode a polypeptide with an mr of 38 600 (p9). the encoded polypeptide has no sequence homology to polypeptides of any other plant reoviruses published previously. an immunological study demonstrated ... | 1995 | 9049345 |
| expression, purification and immunochemical characterization of recombinant bovine beta-lactoglobulin, a major cow milk allergen. | the immunological characteristics of a recombinant beta-lactoglobulin were studied using monoclonal antibodies, polyclonal antiserum and sera from allergic patients. recombinant beta-lactoglobulin (rblg) was expressed in escherichia coli strain dh5alpha and purified as described previously [cho et al. (1994) j. biol. chem. 269, 11 102-11 107]. the method has been modified by adding an immunoaffinity purification step. a quantity of 5-10mg of purified rblg per liter of medium culture can be produ ... | 1996 | 9047377 |
| similar sequence in e. coli. | 1997 | 9038843 | |
| reinterpretation of the ractk1 k+ channel. | the ractk1 cdna cloned from rabbit kidney cortical collecting duct cells was associated with inwardly rectifying ph-regulated k+ channel activity (m. suzuki, k. takahashi, m. [keda, h hayakawa, a. ogawa, y. kawaguchi, and o. sakai. nature lond. 367: 642-645, 1994). the deduced amino acid sequence of the encoded novel polypeptide lacked the signature sequence of a k(+)-selective pore region but predicted a topography suggestive of the inward rectifier k+ channel family. in subsequent articles a r ... | 1997 | 9038842 |
| characterization of the 3' proximal gene of the citrus tristeza closterovirus genome. | the 3' proximal open reading frame (orf 11) in the citrus tristeza virus (ctv) genome potentially encodes a protein of 209 amino acids with an estimated molecular weight of 23 kda (p23). the p23 orf from the severe florida strain t36 of ctv was expressed in escherichia coli, and the expressed protein was used to raise polyclonal antibodies in a rabbit. using these antisera on a western blot, a protein of expected size (23 kda) was detected in tissue extracts from ctv-infected citrus but not from ... | 1997 | 9037736 |
| impact of arachidonic versus eicosapentaenoic acid on exotonin-induced lung vascular leakage: relation to 4-series versus 5-series leukotriene generation. | escherichia coli hemolysin (hlya) is a proteinaceous pore-forming exotoxin that is implicated as a significant pathogenicity factor in extraintestinal e. coli infections including sepsis. in perfused rabbit lungs, subcytolytic concentrations of the toxin evoke thromboxane-mediated vasoconstriction and prostanoid-independent protracted vascular permeability increase (11). in the present study, the influence of submicromolar concentrations of free arachidonic acid (aa) and eicosapentaenoic acid (e ... | 1997 | 9032187 |
| targeted antipeptide antibodies to cytochrome p450 2c18 based on epitope mapping of an inhibitory monoclonal antibody to p450 2c51. | the epitope recognized by the inhibitory monoclonal antibody designated 2f5, which was raised against p450 2c5, was mapped to amino acids 237-260 by immunoblotting using a combination of recombinant antigens and chimeric and partial fusion proteins constructed from rabbit p450s 2c2, 2c4, 2c5, and 2c16, which are recognized by 2f5, and from 2c1 and 2c3, which are not. when the sequence of the epitope for 2f5 (amino acids 237-260) was compared with those of other rabbit 2c p450s, a single lysine r ... | 1997 | 9028867 |
| myosin subfragment-1 is fully equipped with factors essential for motor function. | the sliding velocity of actin filaments propelled by chicken skeletal myosin subfragment-1 (s1) was measured when the tail end of s1 was specifically bound to the glass surface. to achieve the specific binding, a regulatory light chain was replaced by a recombinant fusion protein of biotin-dependent transcarboxylase (bdtc) and chicken gizzard smooth muscle regulatory light chain (cgmrlc). the bdtc-cgmrlc of s1 was then attached to the glass surface using a biotin-avidin system. the velocity of a ... | 1997 | 9020065 |
| immunological analysis of two calpain-like ca2+-dependent proteinases from lobster striated muscles: relationship to mammalian and drosophila calpains. | lobster skeletal muscles contain four ca2+-dependent cysteine proteinases (cdps i, iia, iib, and iii) that degrade myofibrillar proteins. lobster cdps share many properties with calpains from vertebrate tissues, but differ in native mass and subunit composition. recently, cdnas encoding a calpain-like protein (dm-calpain; 91.5 or 94 kda) have been isolated from fruit fly, drosophila melanogaster. to further clarify the relationship between invertebrate cdps and mammalian calpains, antibodies spe ... | 1997 | 9016818 |
| the nonvitellogenic female protein of musca domestica is an adult-specific hexamerin. | during musca domestica vitellogenesis a protein is preferentially synthesized by the female fat body and accumulates in the haemolymph but not in the ovaries. this protein, designated nonvitellogenic female protein (nvfp), was purified and shown to be a hexamer with an m(r) = 430 kda, and subunits of m(r) = 70 kda. the hexamer dissociates into subunits when the ph is elevated from 7.0 to 9.0. two cdna clones, f0 and f2, were isolated and analysed. the 2.2 kb f2 clone has an open reading frame th ... | 1997 | 9013261 |
| casein kinase ii phosphorylates bovine papillomavirus type 1 e1 in vitro at a conserved motif. | the e1 protein of bovine papillomavirus type 1 (bpv-1) is a phosphoprotein which specifically binds and unwinds the virus replication origin by atp-dependent helicase activity. the el protein has been shown to be multiply phosphorylated in vivo, although the sites of modification are incompletely mapped. examination of the predicted amino acid sequence of all available e1 proteins revealed strong conservation between amino acids 25 and 60 of a motif consisting of a serine residue followed by a s ... | 1997 | 9010301 |
| adhesive factor/rabbit 2, a new fimbrial adhesin and a virulence factor from escherichia coli o103, a serogroup enteropathogenic for rabbits. | enteropathogenic escherichia coli-like e. coli strains belonging to serovar o103:k-:h2 and rhamnose-negative biotypes are highly pathogenic diarrhea-inducing strains for weaned european rabbits. we describe here the cloning and sequencing of the major subunit gene of a new fimbrial adhesin, adhesive factor/rabbit 2 (af/r2), which confers on these strains the ability to attach to rabbit enterocytes and to hela cells in a diffuse manner and which is associated with in vivo virulence. the chromosom ... | 1997 | 9009356 |
| the crystal structure of escherichia coli maltodextrin phosphorylase provides an explanation for the activity without control in this basic archetype of a phosphorylase. | in animals, glycogen phosphorylase (gp) exists in an inactive (t state) and an active (r state) equilibrium that can be altered by allosteric effectors or covalent modification. in escherichia coli, the activity of maltodextrin phosphorylase (malp) is controlled by induction at the level of gene expression, and the enzyme exhibits no regulatory properties. we report the crystal structure of e. coli maltodextrin phosphorylase refined to 2.4 a resolution. the molecule consists of a dimer with 796 ... | 1997 | 9009262 |
| recombinant staphylokinase variants with altered immunoreactivity. iii: species variability of antibody binding patterns. | the "charged cluster-to-alanine" substitution variants sakstar(k35a,e38a,k74a,e75a,r77a) and sakstar(k74a,e75a,r77a,e80a,d82a), previously identified as sakstar.m38 and sakstar.m89, respectively, induce less antibody formation in patients than wild-type recombinant staphylokinase (sakstar), but their specific activities are reduced by 50%. therefore, the effect of the reversal of one or more of these substituted amino acids on the ratio of activity to antigenicity was studied. | 1997 | 9008464 |
| unexpected carbohydrate cross-binding by escherichia coli heat-labile enterotoxin. recognition of human and rabbit target cell glycoconjugates in comparison with cholera toxin. | the bacterial protein enterotoxins, cholera toxin (ct) of vibrio cholerae and heat-labile toxin (lt) of escherichia coli, induce diarrhea by enhancing the secretory activity of the small intestine of man and rabbit (animal model). this physiological effect is mediated by toxin binding to a glycolipid receptor, the ganglioside gm1, gal beta 3galnac beta 4(neuac alpha 3)gal beta 4glc beta 1cer. however, lt, but not ct, was recently shown by us to bind also to paragloboside, gal beta 4glcnac beta 3 ... | 1996 | 9007276 |
| impaired bacterial clearance after activation of the complement and coagulation systems. | the aim of this study was to investigate the influence of the activation of the complement and coagulation systems on bacterial clearance and killing capacity of the reticuloendothelial system in rabbits. to enable quantification of the clearance process, defined numbers of exogenous escherichia coli (1.3 x 10(8) colony-forming units) were injected intravenously, after complement activation with inulin-activated rabbit serum (n = 6), after complete defibrination with the snake toxin ancrod (n = ... | 1997 | 8989835 |
| immunocytochemical localization of phenylalanine ammonia-lyase in tissues of populus kitakamiensis. | the polypeptide encoded by the partial fragment of cdna of phenylalanine ammonia-lyase (pal; ec 4.3.1.5), palcdna1 (osakabe et al., 1995, plant sci. 105: 217-226), isolated from populus kitakamiensis (p. sieboldii x p. grandidentata), was expressed in escherichia coli cells. the polypeptide was purified and an antiserum raised against it. the antiserum recognized a protein of 77 kda on nitrocellulose blots after sodium dodecyl sulfate-polyacrylamide gel electrophoresis of total protein and the p ... | 1996 | 8987616 |
| immunodetection of mitochondrial glycerophosphate dehydrogenase (mgdh) by a polyclonal antibody raised against a recombinant mgdh fragment product. | the mitochondrial enzyme glycerophosphate dehydrogenase (mgdh) plays an essential role in the b-cell glucose-sensing device and its activity in islet homogenates is impaired in several animal models of type 2 diabetes. we have now developed a polyclonal antibody, raised against a recombinant mgdh fragment product, that could be used for the immunodetection of mgdh. total rna was isolated from rat pancreatic islets and used in the synthesis of cdna. specific primers were designed that corresponde ... | 1996 | 8986643 |
| once-daily gentamicin therapy for experimental escherichia coli meningitis. | in vitro and in vivo studies have demonstrated that the bacteriologic efficacy of once-daily aminoglycoside therapy is equivalent to that achieved with conventional multiple daily dosing. the impact of once-daily dosing for meningitis has not been studied. using the well-characterized rabbit meningitis model, we compared two regimens of the same daily dosage of gentamicin given either once or in three divided doses for 24 or 72 h. the initial 1 h mean cerebrospinal fluid (csf) gentamicin concent ... | 1997 | 8980753 |
| identification and mapping of a chromosomal gene cluster of borrelia burgdorferi containing genes expressed in vivo. | a clone containing a 6.4 kb borrelia burgdorferi chromosomal dna insert reacted only with sera from patients with lyme disease and not with any normal human or rabbit sera. restriction enzyme analysis indicated that this dna fragment was located on the b. burgdorferi chromosomal map between rpob and p22a; its direction of transcription was towards p22a. sequence analysis suggests that la006 encodes six proteins: three previously described immunodominant lipoproteins of the 39 kda bmp protein fam ... | 1996 | 8978084 |
| human insulin-like growth factor i (igf-i) produced in the mammary glands of transgenic rabbits: yield, receptor binding, mitogenic activity, and effects on igf-binding proteins. | insulin-like growth factor i (igf-i) has acute insulin-like metabolic effects and long-term anabolic actions offering a range of important therapeutic applications. to evaluate a system for large-scale production of this peptide in the mammary glands of transgenic livestock, we generated transgenic rabbits carrying fusion genes in which a synthetic dna coding for human igf-i (higf-i) was placed under the transcriptional control of regulatory elements isolated from the bovine alpha s1-casein (alp ... | 1997 | 8977418 |
| heat-stable serotyping antigens expressed by strains of campylobacter jejuni are probably capsular and not long-chain lipopolysaccharide. | the role of lipopolysaccharide (lps) in the serotyping of campylobacter jejuni based on heat-stable antigens was examined using sds-page and a silver stain for carbohydrate. none of the 32 type strains of camp. jejuni expressed long-chain lps. rabbit antibodies, prepared to 10 selected strains of camp. jejuni, reacted with surface-exposed carbohydrate antigens, which were not lps. this study suggests that the heat-stable antigens of camp. jejuni, which form the basis for the established penner s ... | 1996 | 8972090 |
| chimeric potyvirus-like particles as vaccine carriers. | presentation of subunit vaccines in a highly ordered aggregate form can result in enhanced immune responses. coat protein (cp) monomers of a potyvirus (johnsongrass mosaic virus) when produced in heterologous host expression systems (escherichia coli, yeast and insect cells) self-polymerized to produce potyvirus-like particles (pvlps). the n- and c-terminal regions of potyvirus cp are surface-exposed and are not required for assembly. hybrid cp monomers containing short peptides fused to their n ... | 1996 | 8957674 |
| molecular characterization of a major serotype m49 group a streptococcal dnase gene (sdad). | group a streptococci (gas) express up to four types of secreted dnases. although gas infections are correlated with the production of anti-dnase b antibodies, the roles of dnases in the pathogenesis of gas infections remain unclear. from a lambda library of serotype m49 strain cs101 gas genome, a 2,147-bp fragment expressing dnase activity on an indicator agar was identified and sequenced. one 1,155-bp open reading frame (orf) was identified in this fragment. this orf was found to be 48% identic ... | 1996 | 8945587 |
| o serogroups, biotypes, and eae genes in escherichia coli strains isolated from diarrheic and healthy rabbits. | a total of 305 escherichia coli strains isolated from diarrheic and healthy rabbits in 10 industrial fattening farms from different areas of spain were serotyped, biotyped, and tested for the presence of the eae gene and toxin production. the characteristics found in strains isolated from healthy rabbits were generally different from those observed in e. coli strains associated with disease. thus, strains with the eae gene (74% versus 22%); strains belonging to serogroups o26, o49, o92, o103, an ... | 1996 | 8940455 |
| purification of storage granule protein-23. a novel protein identified by phage display technology and interaction with type i plasminogen activator inhibitor. | type 1 plasminogen activator inhibitor (pai-1) is a key regulator of the fibrinolytic cascade that is stored in a rapidly releasable form within platelet alpha-granules. to identify proteins that may participate in the targeting or storage of this potent inhibitor, this report investigates the applicability of utilizing filamentous bacteriophages to display proteins expressed by cells containing a regulated secretory pathway and their enrichment based upon an interaction with pai-1. for this pur ... | 1996 | 8939962 |
| cholesterol 7alpha-hydroxylase activities from human and rat liver are modulated in vitro posttranslationally by phosphorylation/dephosphorylation. | purified cholesterol 7alpha-hydroxylases (c7alphah) from human and rat liver microsomes, and from transformed escherichia coli expression systems, were incubated with 0.3 mmol/l [gamma-32p] adenosine triphosphate (atp) in the presence and absence of bacterial alkaline phosphatase (ap) or rabbit muscle adenosine 3',5'-cyclic monophosphate (camp)-dependent protein kinase. the amounts of 32p incorporation after separation of human and rat c7alphah proteins by sodium dodecyl sulfate-polyacrylamide g ... | 1996 | 8938182 |
| rapid, efficient, large-scale purification of unfused, non-denatured e7 protein of cottontail rabbit papillomavirus. | cottontail rabbit papillomavirus (crpv) e7 protein is one of the :high risk' papillomavirus e7 oncoproteins that are partially insoluble in aqueous solution. an escherichia coli expression system was used for purification of crpv e7 protein in quantities sufficient for immunologic studies and structural analysis. a glutathione s-transferase (gst)-crpv e7 fusion protein was solubilized in the presence of non-ionic and ionic detergents, and isolated on an affinity column of glutathione sepharose b ... | 1996 | 8919824 |
| interconversion of the androstenedione hydroxylase specificities of cytochromes p450 2b4 and 2b5 upon simultaneous site-directed mutagenesis of four key substrate recognition residues. | based on recent studies of single reciprocal mutants of cytochrome p450 2b4 and the highly related p450 2b5 at positions 114, 294, 363, and 367 [g. d. szklarz, y. q. he, k. m. kedzie, j. r. halpert, and v. l. burnett (1996) arch. biochem. biophys. 327,308-318], a number of multiple mutants were constructed, expressed in escherichia coli, and assayed with androstenedione, progesterone, and benzyloxyresorufin. simultaneous substitutions of ile-114, ser-294, ile-363, and val-367 in cytochrome p450 ... | 1996 | 8914846 |
| effects of n-acetylcysteine on bacterial clearance. | the aim of this study was to investigate whether the oxygen radical scavenger n-acetylcysteine (n-ac) impairs bacterial clearance, thus predisposing the host to increased risk of disease. blood clearance of escherichia coli and organ colonization were investigated in anaesthetized rabbits after pretreatment with n-ac (250 mg kg-1 body weight, n = 16) and in sham-operated animals (n = 12). to enable quantification of the clearance process, defined numbers of exogenous e. coli [1.3 x 108 colony-fo ... | 1996 | 8911862 |
| chronic endotoxemia and endothelium-dependent vasodilation in coronary arteries. | endotoxin acutely decreases the production of nitric oxide, leading to abnormal regulation of coronary vascular tone: however, the effects of chronic endotoxemia on vasomotion are unknown. we therefore tested the hypothesis that chronic, low-level endotoxemia inhibits endothelium-dependent vasodilation. rabbits were continuously infused with a subclinical dose of escherichia coli endotoxin (.6 microgram/24 h, intraperitoneal) or saline for 5 wk. endotoxin at this concentration elicited no signif ... | 1996 | 8902944 |
| hemoglobin exacerbates the ocular inflammatory response to endotoxin. | there is a clinical impression that bleeding into sites of inflammation exacerbates the inflammatory response. it has been hypothesized that hemoglobinic iron (fe) contributes to this response by catalyzing free radical reactions. in the present study, the effects of autologous hemoglobin on the inflammatory response to endotoxin was determined. in addition, the possible contributions of fe to this response was assessed by co-injection of either transferrin or desferrioxamine. | 1996 | 8897058 |
| immune response to non-o157 vero toxin-producing escherichia coli in patients with hemolytic uremic syndrome. | the incidence and time course of the immune response to vero toxin-producing escherichia coli (vtec) other than o157 (o26, o55, o111, o128) were determined in 107 children with enteropathic hemolytic uremic syndrome (hus) by elisa and compared to antibody profiles of 125 healthy pediatric controls and 100 children with community-acquired uncomplicated diarrhea. six of 8 hus patients with non-o157 vtec isolates exhibited a serologic response to the homologous lipopolysaccharide antigen similar to ... | 1996 | 8896505 |
| hemostatic balance during the acute inflammatory reaction; with special reference to antithrombin iii. | inflammatory reaction caused by intramuscular injections of turpentine in 5 rabbits led to an obvious increase of not only plasma fibrinogen and factor viii: c levels, but also of plasma antithrombin iii activity, measured by a chromogenic assay as heparin cofactor. this activity rose from 80% +/- 10,8 (mean +/- sem) before the injection to 123% +/- 6,56 48 hours later. changes affecting plasma fibrinogen level and antithrombin ii activity were much lesser in a group of 5 rabbits given small dos ... | 1995 | 8896078 |
| polycistronic (tri- or bicistronic) phytoreoviral segments translatable in both plant and insect cells. | genomic segment s12 of rice dwarf virus and segment s9 of wound tumor virus, both members of the genus phytoreovirus, have small out-of-phase overlapping open reading frames (orfs). western blot (immunoblot) analysis revealed that rice dwarf virus s12 mrna specified translation products from the large orf and two overlapping small orfs both in rice plant hosts and in spodoptera frugiperda insect cells. these results provide the first example of a tricistronic mrna for a segmented double-stranded ... | 1996 | 8892945 |
| synthesis, processing, and oligomerization of bovine herpesvirus 1 ge and gi membrane proteins. | this study reports the identification and initial characterization of the precursors, modified forms, and oligomers of bovine herpesvirus 1 (bhv-1) gi and ge proteins with polyvalent rabbit serum specific for gi or ge. our experiments used the colorado strain of bhv-1 and mutant viruses with insertions of the escherichia coli lacz gene into the predicted ge and gi reading frames. we also translated the ge and gi open reading frames in vitro and expressed them in uninfected cells using eukaryotic ... | 1996 | 8892910 |
| fine mapping of outer membrane protein p2 antigenic sites which vary during persistent infection by haemophilus influenzae. | antigenic drift of the major outer membrane protein (momp) p2 of nonencapsulated haemophilus influenzae as observed during persistent infections in patients with chronic bronchitis was mimicked in a rabbit model in which h. influenzae persisted in subcutaneous cages. the antigenic drift resulted from amino acid substitutions in potentially surface-exposed loops of momp p2. since in a rabbit model the appearance of antigenic variants was associated with the presence of strain-specific bactericida ... | 1996 | 8890224 |
| mother to fry, successful transfer of immunity against infectious haematopoietic necrosis virus infection in rainbow trout. | we have tested whether immunity can be transferred from a mother fish to its fry. rainbow trout mother fish were inoculated against infectious haematopoietic necrosis virus (ihnv) by intraperitoneal injection of a fragment of the ihnv glycoprotein spanning amino acids 31 to 310. this protein fragment was obtained by isolating the specific cdna from japanese ihnv strain hv7601 and expressing it in escherichia coli. fry from immunized and control fish were exposed to ihnv at various intervals afte ... | 1996 | 8887476 |
| colonisation factors amongst clinical isolates of enterotoxigenic escherichia coli. | the production of heat-labile (lt) and heat-stable (st) enterotoxins, colonisation factor antigens (cfas) and haemagglutinins was investigated amongst 310 escherichia coli (e. coli) isolates obtained from 62 children under the age of five, with diarrhoea. twenty-one isolates were found to produce enterotoxins, of which fifteen (71%) isolates produced st only, 2 (10%) produced lt only and 4 (19%) produced both lt and st. however, none of the isolates demonstrated any of the common cfas identified ... | 1995 | 8882532 |
| the effect of combined rotavirus and escherichia coli infections in rabbits. | in rabbits, experimentally induced rotavirus infection results in soft feces only; thus it is unlikely that it is the sole cause of the severe, often fatal diarrhea of weanling rabbits with which it is associated. to determine whether rotavirus acts synergistically with another pathogen, new zealand white rabbits (10 to 38 weeks old) were inoculated with rotavirus (l:ala:84) and/or escherichia coli 015:h-(rdec-1) via orogastric tube. a single dose of high-titer (10(6) fluorescent focus-forming u ... | 1996 | 8872987 |
| neutrophil activation and lung injury associated with chronic endotoxemia in rabbits. | chronic endotoxemia produces emphysematous lung destruction in several animal models. the present study was designed to examine changes in the polymorphonuclear leukocytes (pmn) and the lung parenchyma of rabbits that received either saline (control, n = 6) or escherichia coli endotoxin (lps, n = 6) 2-3 times weekly for 15 to 28 weeks. peripheral blood was collected just before and after each intravenous injection and lung tissue was processed at the end of the experiment. pmn myeloperoxidase wa ... | 1996 | 8872088 |
| effects of rebamipide on gastric cell damage by helicobacter pylori-stimulated human neutrophils. | helicobacter pylori stimulated human neutrophils to produce oxygen radicals as evidenced by the production of chemiluminescence in the presence of luminol. the capacity of h. pylori to produce oxygen radicals from neutrophils was much higher than that of escherichia coli and staphylococcus aureus and is almost as strong as that of pma. rebamipide (2-(4-chlorobenzoylamino)-3-[2-(1h)-quinolinon-4-yl] propionic acid) suppressed the chemiluminescence produced by h. pylori-stimulated neutrophils and ... | 1995 | 8866835 |
| pentoxifylline does not delay bacterially induced preterm delivery in rabbits. | our purpose was to determine whether pentoxifylline, a potent cytokine inhibitor, would delay bacterially induced preterm delivery in rabbits. | 1996 | 8866298 |
| escherichia coli transcytosis in a caco-2 cell model: implications in neonatal necrotizing enterocolitis. | necrotizing enterocolitis (nec) is a serious gastrointestinal disorder of preterm infants. other than an association with prematurity and gastrointestinal feeding, no single factor or mechanism has been consistently linked to this disease. we have previously demonstrated that escherichia coli isolates obtained from the stool of infants with nec caused nec-like injury in a weanling rabbit ileal loop model; this injury, in turn, could be blocked by coinfection with selected gram(+) bacteria (enter ... | 1996 | 8865278 |
| the calcium-binding protein calretinin-22k, an alternative splicing product of the calretinin gene is expressed in several colon adeno carcinoma cell lines. | an alternatively spliced mrna for the calcium-binding protein calretinin (cr) is present in the colon adenocarcinoma cell line widr. as a consequence of a frame shift, the resulting protein, calretinin-22k (cr-22k), consists of the first 178 amino acids of calretinin followed by a carboxy-terminal peptide of 14 amino acids that is not present in full-length calretinin. antibodies specific for this c-terminal region have been generated by 2 different methods. a peptide corresponding to the specif ... | 1996 | 8864572 |
| vector-derived expression of recombinant rat interleukin-6. | rat interleukin-6 (il-6) cdna, coding for an important inflammation- and immune-regulatory polypeptide cytokine, was cloned into the novel expression vector ph6ex3 which directs the synthesis of inserted genes as a fusion protein with histidine hexapeptide (hh). the resultant vector (pril6.992) was shown to produce significant amounts of recombinant rat il-6 fusion protein with hh at its n-terminus in various strains of escherichia coli. the expression of the hh-il-6 fusion protein was demonstra ... | 1996 | 8860652 |
| isolation and characterization of hemolytic genes from actinobacillus actinomycetemcomitans. | periodontopathic actinobacillus actinomyceremcomitans produces hemolysin and other leukotoxins. in the present study, two distinct clones which lysed horse erythrocytes were isolated by screening genomic dna libraries of a. actinomycetemcomitans atcc 43718 on blood agar plates. dna hybridization analysis indicates that there were two distinct hemolytic genes present. sonicated extracts from both escherichia coli clones possessed hemolytic activities on horse, sheep and human erythrocytes, but no ... | 1996 | 8837475 |
| toxicological evaluation of 1,1,1,2,2-pentafluoroethane (hfc-125). | acute, subacute, and subchronic inhalation toxicity studies, developmental toxicity studies, a cardiac sensitization evaluation, and mutagenicity assays were conducted with pentafluoroethane (hfc-125). in the acute study, rats were exposed to a single concentration of 800,000 ppm for 4 hr. ataxic gait and abnormal respiration were observed during exposure but not after exposure. there was no mortality or other signs of toxicity. repeated exposures of rats to 50,000 ppm, 6 hr/day, 5 days/week for ... | 1995 | 8835232 |
| molecular cloning of clostridium perfringens epsilon-toxin gene and its high level expression in e. coli. | a gene coding for epsilon-toxin was isolated from a field isolate of clostridium perfringens type d by pcr amplification and was cloned under the control of t5 promoter fused with six-histidine tag at the amino terminal end. escherichia coli cells harbouring this construct expressed high levels of the recombinant protein in the form of inclusion bodies. the protein was purified using single step affinity chromatography on a ni(2+)-nitrilotriacetic acid (nta) agarose column. upon immunization of ... | 1996 | 8831683 |
| hydrolysis of n-formylmethionyl chemotactic peptides by endopeptidase 24.11 and endopeptidase 24.15. | endopeptidase 24.11 (ep 24.11), a membrane-bound cell surface enzyme, modulates chemotactic responsiveness of neutrophils to f-met-leu-phe. it is unknown if the enzyme degrades potent formylmethionyl tetrapeptides or if an enzyme with similar activities, endopeptidase 24.15 (ep 24.15), degrades formylated chemotactic peptides. in a study of five formylmethionyl tetrapeptides and f-met-leu-phe, we found that ep 24.11 had high affinity for all peptides evaluated, although it did not effectively de ... | 1996 | 8822504 |
| determination of the in vivo post-antibiotic effects of ciprofloxacin and rifampicin. | a modified rabbit meningitis model is described for determining the pae in vivo, which utilised a self-standing device for repeatedly sampling pure csf. the model allowed the paes of ciprofloxacin and rifampicin on escherichia coli atcc 25922 to be determined following pae induction in vitro, or in vivo in csf after intrathecal injection or during the last 1.5 h of a 7 h iv continuous infusion. the estimated volumes of distribution of ciprofloxacin and rifampicin in csf were 0.68 +/- 0.24 ml and ... | 1995 | 8821597 |
| influence of escherichia coli endotoxin on the pharmacokinetics and respiratory depressant effect of alfentanil in rabbits. | postoperative fever occurs in many surgical patients, due to release of pyrogens into the circulation. pyrogens and fever may influence the pharmacokinetics and effects of drugs. the aim of this study was to investigate the interactions of a pyrogen with the opioid analgesic alfentanil in an animal model. alfentanil was infused over 10 min into 17 conscious rabbits and over 120 min in another 12 animals. some of them had been randomized to pretreatment with escherichia coli endotoxin. arterial p ... | 1996 | 8818989 |
| subcellular location of the tetrapyrrole synthesis enzyme porphobilinogen deaminase in higher plants: an immunological investigation. | a recombinant plasmid, parab8, harbouring the cdna encoding the mature form of the tetrapyrrole synthesis enzyme porphobilinogen deaminase (ec 4.3.1.8; also known as hydroxymethylbilane synthase) from arabidopsis thaliana (l.) heynh. has been constructed, and used to transform escherichia coli. the porphobilinogen deaminase protein from arabidopsis was overexpressed in this strain, and purified to homogeneity (3000-fold) with a yield of 20%. antibodies were raised against the purified plant enzy ... | 1996 | 8818294 |
| expression of thymopoietin beta/lamina-associated polypeptide 2 (tp beta/lap2) and its family proteins as revealed by specific antibody induced against recombinant human thymopoietin. | an expression vector was constructed to produce a common region of human thymopoietin family proteins. the recombinant protein was expressed in escherichia coli as a fusion protein with a biotinylated tag region and purified by affinity chromatography on a monomeric avidin resin. the thymopoietin family-specific antibody was induced in rabbits by immunization with the recombinant fusion protein. western blotting analysis using the antibody revealed that the expression of thymopoietin family prot ... | 1996 | 8806652 |
| the e6 variant proteins e6i-e6iv of human papillomavirus 16: expression in cell free systems and bacteria and study of their interaction with p53. | several species of alternatively spliced mrnas are transcribed from the e6 gene region of human papillomavirus (hpv) 16. these have the coding capacity for either the full length e6 of 151 amino acids (aa) or four truncated variants, e6i-e6iv, of 43-64 aa. as the first step to identify the putative e6 variants and their functions, we generated cdnas corresponding to the various e6 open reading frames (orf) and examined their expression employing in vitro transcription/translation systems and the ... | 1996 | 8806176 |
| l-selectin (cd62l) blockade does not impair peritoneal neutrophil emigration or subcutaneous host defense to bacteria in rabbits. | neutrophil (pmn) recruitment into systemic inflammatory sites in vivo is thought to be initiated by selectin-mediated endothelial adherence. we explored the role of l-selectin (cd62l) in leukocyte emigration following instillation of bacteria into the peritoneum or s.c. skin in rabbits. pretreatment with blocking mab against l-selectin (lam1.3) reduced peritoneal pmn emigration 4 h after i.p. inoculation with 10(10) cfu of escherichia coli by only 17% compared with animals receiving a nonblockin ... | 1996 | 8805657 |
| electrophysiologic monitoring of the effects of soluble virulence factors produced by escherichia coli infection in urine. | the presence of soluble virulence factors in urine infected by escherichia coli has been postulated by recent studies in a rabbit bladder model. these substances may enhance bacterial adherence by damaging the glycosaminoglycan (gag) layer that normally blocks both bacterial adherence and diffusion of solutes to the level of the epithelial membrane. we evaluated the effects of e. coli-infected urine on new zealand white rabbit bladder mucosal permeability by measuring conductance (g) and current ... | 1996 | 8804491 |
| a steady-state and pre-steady-state kinetic analysis of the ntpase activity associated with the hepatitis c virus ns3 helicase domain. | the helicase domain of hepatitis c virus ns3 (genotype 1b) was expressed in escherichia coli and purified to homogeneity. the purified protein catalyzed the hydrolysis of nucleoside triphosphates (ntp) and the unwinding of duplex rna in the presence of divalent metal ion. the enzyme was not selective for the ntp substrate. for example, utp and acyclovir triphosphate were hydrolyzed efficiently by the enzyme. the rate of ntp hydrolysis was stimulated up to 27-fold by oligomeric nucleic acids (na) ... | 1996 | 8798703 |
| antipyretic activity of carboxylatocopper(ii) complexes with salicylate skeleton. | 1996 | 8794476 | |
| immunoreactive core peptides of hepatitis c virus produced in escherichia coli and in vitro dna amplification-restricted transcription-translation system. | three kinds of hepatitis c virus (hcv) core peptides were produced directly and efficiently in e. coli: 1-120 aa of the c region as ncc, 1-157 aa as ncct and 1-190 aa as nccl. these peptides were estimated to be 16, 22 and 24 kda, respectively, by sds-polyacrylamide gel electrophoresis. the processing to produce p22 core protein observed in insect cells and mammalian systems did not occur in e. coli. these peptides were similarly reactive with serum antibody from patients with hepatitis c. a mut ... | 1996 | 8793834 |
| the inhalation toxicology, genetic toxicology, and metabolism of difluoromethane in the rat. | difluoromethane (hfc32) is under development as a replacement for chlorofluorocarbons (cfcs) in some refrigeration applications. it has been evaluated by standard studies of toxicity, developmental toxicity, and genotoxicity. in addition, the metabolism and disposition of hfc32 was investigated and a physiologically based pharmacokinetic (pb-pk) model constructed. inhalation of hfc32 (up to 50,000 ppm) caused no organ-specific effects, but resulted in slight maternal toxicity to the pregnant rat ... | 1996 | 8789790 |
| endotoxin-induced cardiac depression is associated with decreased cardiac dihydropyridine receptors in rabbits. | endotoxin depresses left ventricular (lv) contractility independently of alterations in loading conditions, acidosis, or hypoxia (hung and lew, 1993a). we evaluated if endotoxin-induced lv depression is associated with a decrease in functional l-type calcium channels, as reflected by the number of dihydropyridine receptors measured by [3h]-pn200-110 binding. new zealand white rabbits were instrumented with sonomicrometers to measure the end-systolic pressure-volume relationship after i.v. saline ... | 1996 | 8782078 |
| bacterial production and purification of the fish pituitary hormone somatolactin. | somatolactin, a pituitary hormone belonging to the growth hormone/prolactin family, is produced in the intermediate lobe of teleost pituitary. to date, the functions of this new hormone and the target tissues are unknown. a solea senegalensis somatolactin (sssl) cdna has previously been cloned and isolated. here we have inserted this cdna into a pet-3a plasmid in order to produce recombinant sssl in e. coli bl21 (de3) cells. the protein induced was isolated from inclusion bodies by a solubilizat ... | 1996 | 8776757 |
| escherichia coli expression and substrate specificities of canine cytochrome p450 3a12 and rabbit cytochrome p450 3a6. | high level escherichia coli expression of cytochromes p450 3a12 and 3a6 has facilitated the characterization of proteins which exhibit limited activity as purified hepatic enzymes in reconstituted systems. three 3a12 and two 3a6 constructs modified at the 5'-end to encode the bovine 17 alpha-sequence (barnes et al., proc. natl. acad. sci. u.s.a. 88: 5597-5601, 1991), or related sequences, exhibited expression levels ranging from 2 to 89 nmol of cytochrome p450 liter-1. recombinant canine 3a12 ca ... | 1996 | 8768753 |
| tumor necrosis factor-alpha in macrophages of heart, liver, kidney, and in the pituitary gland. | tumor necrosis factor-alpha (tnfalpha) is an important mediator in bacterial lipopolysaccharide (lps)-induced fever and shock. new data on tnfalpha-producing macrophages in heart, pituitary gland, kidneys and liver in correlation with tnfalpha plasma levels are reported here. in adult rabbits, core temperature and tnfalpha plasma levels are significantly increased at 3 and 24 h after treatment with lps. after a delay of 6-12 h, the number of tnfalpha-containing macrophages, determined by immunoh ... | 1996 | 8766856 |
| extracellular pagc-hlyas fusion protein for the generation and identification of salmonella-specific antibodies. | the outer membrane protein, pagc, of salmonella typhimurium was converted into a secreted protein by linking the 61-amino-acid long, c-terminal signal sequence of the e. coli hemolysin protein (hlyas) to the mature pagc peptide. this pagc-hlyas fusion protein was expressed and efficiently secreted into the culture supernatant by e. coli upon complementation with the hemolysin secretion proteins hlyb and hlyd. polyclonal antibodies raised against this fusion protein not only recognized pagc in th ... | 1996 | 8766698 |
| ribotyping of chryseobacterium meningosepticum: its use as an epidemiological tool and its correlation with serovars. | chryseobacterium meningosepticum (basonym, flavobacterium meningosepticum king 1959) is associated with neonatal meningitis and is isolated from normal and immunocompromised adults. aaf-labelled escherichia coli 16 + 23s rrna was used as a probe for ribotype analysis of 92 clinical isolates from tracheal exsudate, blood culture, cerebrospinal fluid, urine and pus. the 92 isolates belonged to the 15 described serovars of c. meningosepticum, and included 21 strains isolated during an outbreak in a ... | 1996 | 8763627 |
| the proteins encoded by the rbs operon of escherichia coli: ii. use of chimeric protein constructs to isolate and characterize rbsc. | chimeric genes encoding full-length copies of rbsa and rbsc connected by segments coding for short bridge peptides were constructed and expressed in escherichia coli. surprisingly, the chimeric genes complemented the strain in which rbsa and rbsc were deleted. the chimeric proteins were overproduced, and the products were purified by affinity chromatography. in order to obtain highly purified protein, a poly-his leader peptide was incorporated so that ni-chelate affinity chromatography could be ... | 1996 | 8762141 |
| cytoplasmic localization of the white phase-specific wh11 gene product of candida albicans. | cells of candida albicans wo-1 switch frequently, spontaneously and reversibly between a white and opaque phase. the white-opaque transition involves the regulation of phase-specific genes. in the white budding phase, cells express the white phase-specific gene wh11, which encodes a protein with homology to the heat shock protein hsp12 of saccharomyces cerevisiae. a recombinant wh11 protein has been synthesized, purified to apparent homogeneity and used to generate a rabbit polyclonal antiserum. ... | 1996 | 8760936 |