Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| the ectodomain of a novel member of the immunoglobulin subfamily related to the poliovirus receptor has the attributes of a bona fide receptor for herpes simplex virus types 1 and 2 in human cells. | we report on the functional cloning of a hitherto unknown member of the immunoglobulin (ig) superfamily selected for its ability to confer susceptibility to herpes simplex virus (hsv) infection on a highly resistant cell line (j1.1-2 cells), derived by exposure of bhktk- cells to a recombinant hsv-1 expressing tumor necrosis factor alpha (tnf-alpha). the sequence of herpesvirus ig-like receptor (higr) predicts a transmembrane protein with an ectodomain consisting of three cysteine-bracketed doma ... | 1998 | 9811737 |
| novel entry pathway of bovine herpesvirus 1 and 5. | herpesviruses enter cells by a yet poorly understood mechanism. we visualized the crucial steps of the entry pathway of bovine herpesvirus 1 (bhv-1) and bhv-5 by transmission and scanning electron microscopy, employing cryotechniques that include time monitoring, ultrarapid freezing, and freeze substitution of cultured cells inoculated with virus. a key step in the entry pathway of both bhv-1 and bhv-5 is a unique fusion of the outer phospholipid layer of the viral envelope with the inner layer ... | 1998 | 9811689 |
| inactivation of viruses by beta-propiolactone in human cryo poor plasma and igg concentrates. | virus inactivation by cold treatment with beta-propiolactone (bpl) was investigated in human cryo poor plasma and purified igg concentrates spiked with relevant human viruses or appropriate animal model viruses. the samples were treated with 0.1 or 0.25% bpl for 300 or 480 min, respectively. residual infectivity was determined by standard microtitration assays on tissue culture cells. the inactivation of all viruses tested was more effective in igg than in plasma. igg: r1=4-5.5 log10 for vesicul ... | 1998 | 9811521 |
| a high sensitivity-nested pcr assay for bhv-1 detection in semen of naturally infected bulls. | several different pcr protocols for the detection of bovine herpesvirus-1 (bhv-1) in bovine semen, are available in the literature. most of them are rather laborious and the majority were performed on laboratory samples, artificially contaminated semen or semen provided from experimentally inoculated animals. furthermore, to obtain higher levels of sensitivity, additional dot-blot procedures are frequently necessary. we describe the detection of bhv-1 in bovine semen and the supernatant of cell ... | 1998 | 9810617 |
| effect of vaccine-induced immune activation on the performance of early-weaned pigs. | two groups of 96 pigs were studied to determine the influence of weaning age, nursery site and a challenge to their immune system on their performance. the weaning ages were 11 to 16 days and 16 to 21 days. one nursery was on-site and the second nursery was off-site. immune activation was stimulated by the administration of infectious bovine rhinotracheitis virus (ibr) vaccine to half of the pigs at each site. serum virus neutralisation titres to ibr and total immunoglobulins were monitored in s ... | 1998 | 9795401 |
| an epidemiological and economic simulation model to evaluate the spread and control of infectious bovine rhinotracheitis in the netherlands. | bovine herpesvirus type i (bhv1), causing infectious bovine rhinotracheitis (ibr), was introduced in the netherlands in 1971. in 1993, about 42% of the dairy cows had antibodies against bhv1. in the future, stricter requirements are anticipated regarding the health status of exported breeding cows and material. to support policymakers in their decisions on ibr eradication, a simulation model was developed in which the epidemiological and economic consequences of various control strategies were e ... | 1998 | 9785377 |
| comparative molecular epidemiological investigation on different bovine herpes viruses. | eight bulgarian bovine herpes viruses, two hungarian herpes viruses 1a, 3a, calves isolate named mramor, buffalo isolate 723 and two referents bhv 1 strains were investigated by restrictase fragment pattern analysis. migration profile of viral dna by using different restrictase enzymes hpa i, bamh i and hind iii were compared. clearly differences among two hungarian strains, calves isolate mramor, buffalo isolate 723 and 8 bulgarian and two referents bhv 1 strain was observed. the strain sartze ... | 1998 | 9775356 |
| construction and characterization of e3-deleted bovine adenovirus type 3 expressing full-length and truncated form of bovine herpesvirus type 1 glycoprotein gd. | using the homologous recombination machinery of e. coli, a 1.245-kb deletion was introduced in the e3 region of bovine adenovirus 3 (bav3) genomic dna cloned in a plasmid. transfection of the restriction enzyme-excised, linear e3-deleted bav3 genomic dna into primary fetal bovine retina cells produced infectious virus (bav3. e3d), suggesting that all the e3-specific open reading frames are nonessential for virus replication in vitro. using a similar approach, we constructed replication-competent ... | 1998 | 9770436 |
| induction of immunity in the respiratory tract and protection from bovine herpesvirus type 1 infection by different routes of immunization with recombinant adenovirus. | to investigate the capability of different routes of immunization with replication-competent recombinant adenovirus to induce antigen-specific antibody responses, we immunized cotton rats with a human adenovirus type 5 (had5) vector expressing the glycoprotein d (gd) of bovine herpesvirus type 1 (bhv-1) (gd-de3). different routes of mucosal and systemic immunization (intraduodenal-oral, intraduodenal, intranasal and intradermal) with gd-de3 stimulated similar levels of gd-specific igg in the ser ... | 1998 | 9765030 |
| a protein encoded by the latency-related gene of bovine herpesvirus 1 is expressed in trigeminal ganglionic neurons of latently infected cattle and interacts with cyclin-dependent kinase 2 during productive infection. | despite productive viral gene expression in the peripheral nervous system during acute infection, the bovine herpesvirus 1 (bhv-1) infection cycle is blocked in sensory ganglionic neurons and consequently latency is established. the only abundant viral transcript expressed during latency is the latency-related (lr) rna. lr gene products inhibit s-phase entry, and binding of the lr protein (lrp) to cyclin a was hypothesized to block cell cycle progression. this study demonstrates lrp is a nuclear ... | 1998 | 9733854 |
| effects of long-term infection with bovine immunodeficiency virus and/or bovine leukemia virus on antibody and lymphocyte proliferative responses in cattle. | immune responses were examined in cattle between 3-5 years after experimental inoculation with bovine immunodeficiency virus (big) and/or bovine leukemia virus (blv). lymphocyte proliferative responses to con a or to allogeneic lymphocytes with foreign major histocompatibility complex molecules (allo mhc) were determined by 3h-thymidine incorporation assays. antigen-specific antibody and lymphocyte proliferative responses were measured following vaccination with tetanus toxoid (tt) and bovine he ... | 1998 | 9730220 |
| bovine herpesvirus type 1 glycoprotein h is essential for penetration and propagation in cell culture. | bovine herpesvirus type 1 (bhv-1) glycoprotein h (gh) is a structural component of the virion which forms a complex with glycoprotein gl. to study the role of bhv-1 gh in the virus infectious cycle, a gh null mutant was constructed in which the gh coding sequences were deleted and replaced by the escherichia coli lacz cassette. the bhv-1 gh null mutant was propagated in trans-complementing mdbk cells, stably transfected with plasmid pmep4 containing the bhv-1 gh gene under the control of the ind ... | 1998 | 9714247 |
| critical factors affecting the diagnostic reliability of enzyme-linked immunosorbent assay formats. | this paper aims to evaluate different formats of the enzyme-linked immunosorbent assays (elisas) for detection of virus-specific antibodies and focuses on factors that may influence the diagnostic reliability of such tests. newly developed and well-established elisas for detection of infections of bovine herpesvirus 1 (bhv1), bovine respiratory syncytial virus (brsv), classical swine fever virus (csfv), pseudorabies virus (prv) and bovine viral diarrhoea virus (bvdv) are used as examples. differ ... | 1998 | 9713894 |
| occurrence of some viruses and bacteria involved in respiratory diseases of ruminants in hungary. | serological surveys, virological and bacteriological investigations were carried out on large populations of lambs and calves kept in intensive fattening units on hungarian large-scale farms, in order to study the occurrence of viruses and bacteria involved in the respiratory disease complex. the investigations were carried out in stocks where 5-20% of the young animals succumbed to acute or chronic pneumonia every year. serological surveys revealed that seropositivity to bovine adenovirus type ... | 1998 | 9713141 |
| attachment but not penetration of bovine herpesvirus 1 is necessary to induce apoptosis in target cells. | bovine herpesvirus 1 (bhv-1) induces apoptotic cell death in bovine peripheral blood mononuclear cells and b-lymphoma cells. using a bhv-1 glycoprotein h null mutant, we have demonstrated that although penetration of bhv-1 is not required, attachment of bhv-1 viral particles is essential for the induction of apoptosis. | 1998 | 9696867 |
| alternative splicing of the latency-related transcript of bovine herpesvirus 1 yields rnas containing unique open reading frames. | the latency-related transcript (lrt) of bovine herpesvirus 1 (bhv-1) is the only abundant viral rna detected during latency. a previous study (a. hossain, l. m. schang, and c. jones, j. virol. 69:5345-5352, 1995) concluded that splicing of polyadenylated [poly(a)+] and splicing of nonpolyadenylated [poly(a)-] lrt are different. in this study, splice junction sites of lrt were identified. in trigeminal ganglia of acutely infected calves (1, 7, or 15 days postinfection [p.i.]) or in latently infec ... | 1998 | 9696825 |
| cd4(+) cytotoxic t-lymphocyte activity against macrophages pulsed with bovine herpesvirus 1 polypeptides. | bovine herpesvirus 1 (bhv-1) induces immune suppression, but the mechanisms for suppression are not well identified. we examined the induction and activity of bhv-1-specific cytolytic cd4(+) t lymphocytes (ctl) by stimulating peripheral blood mononuclear cells (pbmc) of cattle immunized with attenuated live bhv-1. cytolytic effector cells were primarily cd4(+) t lymphocytes and lysed autologous, but not allogeneic, macrophages infected with bhv-1 or pulsed with bhv-1 polypeptides. apoptosis of b ... | 1998 | 9696796 |
| infectious bovine keratoconjunctivitis: a review. | the economic impact of infectious bovine keratoconjunctivitis (ibk) warrants continued investigation of the mechanisms by which moraxella bovis survives on and colonizes the corneal surface. virulent strains of m bovis produce hemolysin and exhibit different plasmid profiles than nonvirulent strains. interactions among host, environment, vector, season, and concurrent infection influence the prevalence of ibk. mycoplasma sp. or infectious bovine rhinotracheitis virus may enhance or hasten the di ... | 1998 | 9686385 |
| resistance to bovine respiratory syncytial virus (brsv) induced in calves by a recombinant bovine herpesvirus-1 expressing the attachment glycoprotein of brsv. | the ability of a bovine herpesvirus-1 (bhv-1) recombinant expressing the g protein of bovine respiratory syncytial virus (brsv) to protect against brsv infection was examined in calves. a synthetic g gene was inserted behind the ge promoter of bhv-1 to give a ge-negative, bhv-1/g recombinant. gnotobiotic calves, vaccinated intranasally and intratracheally with bhv-1/g were challenged 6 weeks later with the snook strain of brsv. as controls, calves were vaccinated with a ge-negative mutant of bhv ... | 1998 | 9680140 |
| immediate-early protein bicp22 of bovine herpesvirus 1 trans-represses viral promoters of different kinetic classes and is itself regulated by bicp0 at transcriptional and posttranscriptional levels. | bovine herpesvirus 1 (bhv-1) encodes four immediate-early (ie) proteins. the transactivators bicp0 and bicp4 are key regulatory elements in viral replication, and circ is a myristylated virion component, whereas bicp22--originating from a spliced 1.7-kb transcript synthesized with dual ie and late kinetics--has not yet been characterized as a protein. in this study, western blot and immunofluorescence analysis using antisera against a c-terminal oligopeptide revealed major 50-kda and minor 35-kd ... | 1997 | 9672606 |
| construction of a viable bhv1 mutant lacking most of the short unique region. | the 8.2 kb hindiii k-fragment of bovine herpesvirus 1 (bhv1) lies entirely within the short unique region of the genome and contains all or parts of 7 coding regions. we have probed this fragment for the presence of nonessential genes by a simple, rapid method of insertional mutagenesis. our analysis indicates that all the genes present in the k-fragment, except the glycoprotein d gene, are nonessential for replication of bhv1 in tissue culture. after inserting a copy of the glycoprotein d gene ... | 1997 | 9672601 |
| a cell surface protein with herpesvirus entry activity (hveb) confers susceptibility to infection by mutants of herpes simplex virus type 1, herpes simplex virus type 2, and pseudorabies virus. | certain mutant strains of herpes simplex virus type 1 (hsv-1) are unable to infect cells in which entry is dependent on hvem, the previously described herpesvirus entry mediator designated here as herpesvirus entry protein a (hvea). these mutant viruses can infect other cells where entry is apparently dependent on other co-receptors. the mutant virus hsv-1(kos)rid1 was used to screen a human cdna expression library for ability of transfected plasmids to convert resistant chinese hamster ovary ce ... | 1998 | 9657005 |
| evaluation of baculovirus-expressed bovine herpesvirus-1 (bhv-1) glycoproteins for detection and analysis of bhv-1-specific antibody responses. | baculovirus (autographa californica nuclear polyhedrosis)-expressed bovine herpesvirus-1 (bhv-1) glycoproteins b (gb), gc, and gd were developed and characterized. the recombinant proteins retained their antigenic properties as determined by immunoblotting against monoclonal antibodies. the proteins were examined as antigens for detection of bhv-1 infection and for the analysis of antibody responses to the individual viral proteins. a total of 115 bovine serum samples were tested for their react ... | 1998 | 9646475 |
| reactivation of latent bovine herpesvirus 1 in cattle seronegative to glycoproteins gb and ge. | six heifers were vaccinated intranasally with the live bovine herpesvirus 1 (bhv1) temperature-sensitive (ts) vaccine strain rbl106 within 3 weeks of birth. these calves most likely still had maternal antibodies against bhv1. thereafter, these heifers were vaccinated several times with an experimental bhv1 glycoprotein-d (gd) subunit vaccine. at the age of 3 years these 6 heifers were seronegative in the bhv1 gb and ge blocking elisas, but had neutralizing antibodies against bhv1, probably induc ... | 1998 | 9646441 |
| a comparative study of serological tests for use in the bovine herpesvirus 1 eradication programme in the netherlands. | we compared a gb-elisa, a ge-elisa and a danish test system (consisting of a blocking and an indirect elisa) for their specificity and sensitivity to detect antibodies against bhv1. the danish test system showed the highest sensitivity and the ge-elisa the lowest; the gb-elisa showed an intermediate sensitivity. if the doubtful zone (25-50% blocking) of the gb-elisa was considered as positive (gb-elisa+), the sensitivity almost reached that of the danish test system. the specificity of all tests ... | 1998 | 9631527 |
| virulence, immunogenicity and reactivation of bovine herpesvirus 1 mutants with a deletion in the gc, gg, gi, ge, or in both the gi and ge gene. | within the framework of developing a marker vaccine against bovine herpesvirus 1 (bhv1), several mutants with deletions in non-essential glycoprotein genes were constructed. glycoprotein gc, gg, gi and ge single deletion mutants, a gi/ge double deletion mutant and a ge frame-shift mutant were made. the virulence and immunogenicity of these mutants were evaluated in specific-pathogen-free calves. except for the gc deletion mutant, all mutants were significantly less virulent than the parental wil ... | 1998 | 9627937 |
| bovine herpesvirus-1 infection affects the peptide transport activity in bovine cells. | infection of cattle with bovine herpesvirus-1 (bhv-1) impairs the cell-mediated immune response (cmi) of the affected host. we investigated the location of interference of bhv-1 with the major histocompatibility complex (mhc) class i antigen presentation pathway by employing an assay that allows assessment of the peptide transport activity of the transporter associated with antigen presentation (tap) from the cytoplasm into the endoplasmic reticulum (er). we found a considerable down-regulation ... | 1998 | 9617772 |
| entry of alphaherpesviruses mediated by poliovirus receptor-related protein 1 and poliovirus receptor. | a human member of the immunoglobulin superfamily was shown to mediate entry of several alphaherpesviruses, including herpes simplex viruses (hsv) 1 and 2, porcine pseudorabies virus (prv), and bovine herpesvirus 1 (bhv-1). this membrane glycoprotein is poliovirus receptor-related protein 1 (prr1), designated here as hvec. incubation of hsv-1 with a secreted form of hvec inhibited subsequent infection of a variety of cell lines, suggesting that hvec interacts directly with the virus. poliovirus r ... | 1998 | 9616127 |
| further investigations on the efficacy of a non-specific defence inducer evaluated in calves exposed to infectious bovine rhinotracheitis virus. | six calves were given the immunomodulator baypamun and housed together with another six calves of which, three were experimentally infected with bovine herpesvirus-1 (bhv-1), whereas the remaining three served as untreated controls. the three experimentally infected calves as well as the three controls developed clinical signs of the typical acute form of infectious bovine rhinotracheitis (ibr). of the calves treated with baypamun, those that had only one injection of the immunomodulator, either ... | 1998 | 9611684 |
| detection of bovine herpesvirus 1 (bhv-1) genomic sequences in bovine semen inoculated with bhv-1 by polymerase chain reaction. | polymerase chain reaction (pcr) technique was applied to detect bhv-1 in bovine semen inoculated with bhv-1. the technique was found to be 10(6) times more sensitive than a non-isotopic dot-blot hybridization method in detecting viral genomic dna. of the three primer pairs used, the one chosen from glycoprotein gc appeared to be most sensitive as it could detect up to 0.01 tcid50 of bhv-1 in the semen. the technique could be useful in screening breeding bulls or samples of frozen semen prior to ... | 1997 | 9607088 |
| an inactivated ge-negative marker vaccine and an experimental gd-subunit vaccine reduce the incidence of bovine herpesvirus 1 infections in the field. | an inactivated glycoprotein e-negative vaccine and an experimental glycoprotein d-subunit vaccine against bovine herpesvirus 1 (v1) were examined for their effectiveness in a randomized, double-bline, placebo-controlled field trial comprising 130 dairy farms. the use of these marker vaccines enabled us to monitor the incidence of infections in vaccinated populations. the aims of this trial were to evaluate whether these vaccines: (1) reduce the proportion of outbreaks in dairy herds; and (2) red ... | 1998 | 9607041 |
| risk factors for existence of bovine herpes virus 1 antibodies on nonvaccinating dutch dairy farms. | a more closed farming system may prevent introduction of infectious diseases on dairy farms and can be a good starting point for control of these diseases. data were available on the presence of bovine herpes virus 1 (bhv1) antibodies in bulk milk and/or blood samples of dutch dairy farms. furthermore, information about the possible risk factors for introduction of infectious diseases was collected on 214 of these dairy farms. data of 107 farms which had been never vaccinated against bhv1 remain ... | 1998 | 9604262 |
| milk production and reproduction during a subclinical bovine herpesvirus 1 infection on a dairy farm. | this study describes an outbreak of bovine herpesvirus 1 (bhv1) infections in a dairy herd with special reference to disease symptoms, reproductive performance and milk production losses. the study was carried out with a dairy herd consisting of 98 lactating animals. all animals were housed in the same freestall barn with intensive contact between all animals. an outbreak of bhv1 was induced by injecting three seropositive cows with dexamethasone. during the outbreak, no clinical signs were obse ... | 1998 | 9604259 |
| development of a syngeneic bovine fibroblast cell line: implications for the study of bovine cytotoxic t lymphocytes. | the study of t-cell-mediated cytotoxicity in domestic animals, especially in cattle, has been hampered by the lack of proper restimulatory as well as target systems. while the currently available bovine cell lines have not been typed for the major histocompatibility complex (mhc) class i molecules they express, methods to derive lines of cells obtained from animals that are mhc-typed have not been thoroughly explored. in the present study, we describe a method for the development of cell lines f ... | 1998 | 9586696 |
| prevalence of antibodies to bovine virus diarrhoea virus and other viruses in bulk tank milk in england and wales. | bulk tank milk samples from 1070 dairy herds in england and wales were tested by elisa for antibodies to bovine virus diarrhoea virus (bvdv). a subset of 341 herds was tested by elisa for antibodies to bovine herpesvirus 1 (bhv-1), bovine respiratory syncytial virus (brsv) and bovine coronavirus (bcv). none of the herds had less than 40 dairy cows and none had been vaccinated against bvdv. the prevalence of bvdv antibody-positive herds in the national population was estimated at 95 per cent and ... | 1998 | 9586130 |
| humoral and cell-mediated immune responses of beef and dairy cattle experimentally infested with psoroptes ovis. | to compare cellular and humoral immune responses of beef (belgian white and blue [bwb]) and dairy (friesian-holstein [fh]) cattle to psoroptes ovis infestation and to determine whether p ovis infestation impaired immune responses to infectious bovine rhinotracheitis virus (ibr) vaccine or an immunogenic protein (keyhole-limpet hemocyanin [klh]). | 1998 | 9582960 |
| construction and characterization of a stably transformed hela cell line in which the expression of bovine herpesvirus 1 icp0 (bicp0) is induced by tetracycline. | to explore the effects bicp0 (a principal transactivator of bhv-1 gene expression) on viral promoter elements, we established a cell line in which the expression of bicp0 is regulated by tetracycline. a hybrid promoter containing reiterated copies of the tet-operator (tet-o) and a minimal herpesviral alpha gene transinducing factor (alpha tif) responsive element (minimal human cytomegalovirus immediate early promoter) was fused to the bicp0 gene and used to transform a hela cell line which expre ... | 1998 | 9580095 |
| bovine herpesvirus 1-induced apoptosis: phenotypic characterization of susceptible peripheral blood mononuclear cells. | bovine herpesvirus 1 (bhv-1), a member of the alphaherpesvirinae, induces apoptotic cell death in peripheral blood mononuclear cells (pbmc). to investigate the process by which bhv-1 induces apoptosis, we determined the susceptibility of the three main pbmc subpopulations to bhv-1-induced apoptosis. this study shows that bhv-1 can induce apoptosis individually in t lymphocytes, b lymphocytes and monocytes. this conclusion is based on the following findings: (i) bhv-1 substantially reduces the pe ... | 1998 | 9572546 |
| intradermal immunization with a bovine herpesvirus-1 dna vaccine induces protective immunity in cattle. | although intramuscular (i.m.) injection of dna encoding glycoprotein d (gd) of bovine herpesvirus-1 (bhv-1) induces immune responses in cattle, this route of delivery is inefficient. here we assessed three parameters that may enhance the efficacy of a gd dna vaccine in cattle. first, the immune response generated by i.m. injected plasmid expressing a secreted form of gd (tgd) was determined and found to be very similar in magnitude to the response induced by gd-expressing plasmid. secondly, gd- ... | 1998 | 9568979 |
| simple and rapid purification of alphaherpesviruses by chromatography on a cation exchange membrane. | a simple and rapid method is described for the purification of two alphaherpesviruses, pseudorabies virus (prv) and bovine herpesvirus 1, by chromatography on a cation exchange membrane. cell culture supernatants were passed over a sulfonic-acid modified filter membrane and virions were eluted with a potassium chloride-containing buffer. over 85% of the virus was eluted within a single fraction and specific infectivity of the resulting virus preparation was over 10-fold higher than that of sucro ... | 1998 | 9562416 |
| detection of a novel bovine lymphotropic herpesvirus. | degenerate pcr primers which amplify a conserved region of the dna polymerase genes of the herpesvirus family were used to provide sequence evidence for a new bovine herpesvirus in bovine b-lymphoma cells and peripheral blood mononuclear cells (pbmc). the sequence of the resultant amplicon was found to be distinct from those of known herpesvirus isolates. alignment of amino acid sequences demonstrated 70% identity with ovine herpesvirus 2, 69% with alcelaphine herpesvirus 1, 65% with bovine herp ... | 1998 | 9557713 |
| the class ii membrane glycoprotein g of bovine respiratory syncytial virus, expressed from a synthetic open reading frame, is incorporated into virions of recombinant bovine herpesvirus 1. | the bovine herpesvirus 1 (bhv-1) recombinants bhv-1/eg(ori) and bhv-1/eg(syn) were isolated after insertion of expression cassettes which contained either a genomic rna-derived cdna fragment (bhv-1/eg(ori)) or a modified, chemically synthesized open reading frame (orf) (bhv-1/eg(syn)), which both encode the attachment glycoprotein g of bovine respiratory syncytial virus (brsv), a class ii membrane glycoprotein. northern blot analyses and nuclear runoff transcription experiments indicated that tr ... | 1998 | 9557663 |
| [introduction of bhv1 on dairy farms. risk assessment by cattle farmers and veterinarians]. | a study is being carried out at wageningen agricultural university together with, among others, the animal health service to determine the possibilities and economic consequences of a more closed farming system for (dutch) dairy farms. three identical workshops, held in the evening, were organized as part of the study. the opinion of farmers and their veterinarians on the importance of risk factors for the introduction of diseases on a farm was determined, using bovine herpes virus type 1 (bhv1) ... | 1998 | 9537105 |
| [spread of an intramuscularly administered live ge-negative bhv1 marker vaccine in 2 cattle farms]. | cattle are vaccinated with a live ge-negative bhv1-marker vaccine as a part of a programme to eradicate bovine herpes virus 1 in the netherlands. to investigate whether this vaccine is transmitted to unvaccinated cattle in a herd after intramuscular administration, cattle from two herds were vaccinated: of a herd of 114 bhv1-antibody negative cattle on one farm, 45 animals were vaccinated, and of a dairy herd of 55 cattle in another farm, all 10 bhv1-positive animals were vaccinated. vaccination ... | 1998 | 9537086 |
| epidemiological characteristics of bovine herpesvirus 1 infections determined by bulk milk testing of all dutch dairy herds. | samples of bulk milk were taken from all 33,636 dutch dairy herds in november 1994 and tested for the presence of bovine herpesvirus 1 (bhv-1) antibodies with a gb-blocking elisa. sixteen per cent of the herds had a negative bhv-1 status in the bulk milk. farms with only dairy cows were 1.9 times more likely to have a negative or weakly positive bhv-1 status than herds which also had beef/veal animals. farms in areas containing less than one herd/km2 were 1.5 times more likely to have a negative ... | 1998 | 9533279 |
| evaluation of a single dilution elisa system for detection of seroconversion to bovine viral diarrhea virus, bovine respiratory syncytial virus, parainfluenza-3 virus, and infectious bovine rhinotracheitis virus: comparison with testing by virus neutralization and hemagglutination inhibition. | a single-dilution quantitative enzyme-linked immunosorbent assay (elisa) system, based on commercial elisa kits, for the simultaneous detection of seroconversion to bovine viral diarrhea virus (bvdv), bovine respiratory syncytial virus (brsv), parainfluenza-3 virus (pi3v), and infectious bovine rhinotracheitis virus (ibrv) was evaluated by testing acute and convalescent serum pairs from 564 cattle in 145 outbreaks of respiratory disease. seroconversion to bvdv, brsv, pi3v and ibrv was detected i ... | 1998 | 9526859 |
| functional expression of the bovine herpesvirus 1 alkaline deoxyribonuclease (ul12) in escherichia coli. | sequence analysis within the unique long segment of the bovine herpesvirus 1 (bhv-1; infectious bovine rhinotracheitis virus) genome identified an open reading frame whose deduced protein product of 487 amino acids exhibited homology to alkaline deoxyribonucleases (dnases) of other herpesviruses. to determine this bhv-1 gene product has nuclease activity, the gene designated ul12 was inserted into the vector pet-28a(+) and expressed in escherichia coli as an oligohistidine-tagged protein. upon i ... | 1996 | 9526549 |
| bovine herpesvirus 1 glycoprotein m forms a disulfide-linked heterodimer with the u(l)49.5 protein. | nine glycoproteins (gb, gc, gd, ge, gg, gh, gi, gk, and gl) have been identified in bovine herpesvirus 1 (bhv-1). gm has been identified in many other alpha-, beta-, and gammaherpesviruses, in which it appears to play a role in membrane penetration and cell-to-cell fusion. we sought to express bhv-1 open reading frame u(l)10, which encodes gm, and specifically identify the glycoprotein. we corrected a frameshift error in the published sequence and used the corrected sequence to design coterminal ... | 1998 | 9525625 |
| nucleotide sequence of canine herpesvirus homologues of herpes simplex virus type 1 us2, us3, glycoproteins i and e, us8.5 and us9 genes. | the partial nucleotide sequence of two bamhi fragments that span the unique short region (us), terminal repeat region (tr) and internal repeat region (ir) of canine herpesvirus (chv) has been determined. data obtained revealed several open reading frames (orf's) identified as the us2, us3, gi, ge and us9 homologues of herpes simplex virus type 1 (hsv1). the chv homologues also show significant identity in amino acid sequence with those encoded by feline herpesvirus type 1 (fhv1), bovine herpesvi ... | 1997 | 9524817 |
| health evaluation of black-faced impala (aepyceros melampus petersi) using blood chemistry and serology. | as part of ongoing ecological studies of black-faced impala (aepyceros melampus petersi) in northern namibia, blood samples were collected and analyzed from 26 animals captured for translocation in 1992. all animals appeared to be in good condition and no abnormality was noted during physical examination. serum chemistry and mineral levels were measured and correlated with the results of bacterial and viral serology and were within the normal ranges for domestic ruminants. antibody titers for in ... | 1997 | 9523628 |
| neuropathology of bovine herpesvirus type 5 (bhv-5) meningo-encephalitis in a rabbit seizure model. | the suitability of a rabbit seizure model for studying the neuropathogenesis of bovine herpesvirus type 5 (bhv-5) encephalitis was evaluated. intranasal administration of bhv-5 (strain tx89) together with intramuscular administration of dexamethasone produced seizures in 70% of rabbits tested and meningo-encephalitis in 100%. infectious bhv-5 was consistently isolated from the following sites: olfactory bulb; anterior cortex, containing the frontal cortex, olfactory tract and anterior portion of ... | 1997 | 9502267 |
| pseudorabies virus glycoprotein gk is a virion structural component involved in virus release but is not required for entry. | the pseudorabies virus (prv) gene homologous to herpes simplex virus type 1 (hsv-1) ul53, which encodes hsv-1 glycoprotein k (gk), has recently been sequenced (j. baumeister, b. g. klupp, and t. c. mettenleiter, j. virol. 69:5560-5567, 1995). to identify the corresponding protein, a rabbit antiserum was raised against a 40-kda glutathione s-transferase-gk fusion protein expressed in escherichia coli. in western blot analysis, this serum detected a 32-kda polypeptide in prv-infected cell lysates ... | 1998 | 9499048 |
| tumor necrosis factor beta is a major source of antibody-activated effector cells in the killing of virally infected target cells. | we have extended our previous work on cross-linking cd4 molecules using specific monoclonal-antibody-induced antigen-nonspecific major-histocompatibility-complex unrestricted killing by cd4+ t cells of virally infected target cells. the killing activity of antibody-activated cd4+ t cells was completely blocked by herbimycin a, a protein tyrosine kinase (ptk) inhibitor, but not by bisindolylmaleimide, a protein kinase c inhibitor. herbimycin-a-treated human or bovine peripheral blood cd4+ t cells ... | 1997 | 9491850 |
| a serological survey of bovine herpesvirus-1 infection in selected dairy herds in northern and central italy. | serum samples from a total of 6979 dairy cattle from 55 herds in northern italy (51 herds) and central italy (4 herds), were examined by the serum neutralization test for the presence of antibody to bovine herpesvirus-1 (bhv-1). it was found that 84.31% of the farms selected in northern italy and all the farms from central italy had seropositive animals at titers of 1:4 or higher. the prevalence of infection was essentially the same among the cattle populations of the two selected areas of the c ... | 1997 | 9481516 |
| the humoral immune response in cattle after immunization with a multivalent ibr/pi3/pasteurella haemolytica a1 leukotoxin vaccine. | a multivalent vaccine consisting of inactivated bovine herpes virus-1 (bhv-1), also known as infectious bovine rhinotracheitis virus (ibr), para-influenza type-3 virus (pi3) and the leukotoxin of pasteurella haemolytica a1, were combined with the addition of aluminium hydroxide as adjuvant, and administered to post-weaned calves, and the serum tested for seroconversion to each antigen. two groups of calves (n = 150 and n = 68) were used and were randomly divided into three subgroups. the first g ... | 1997 | 9467176 |
| detection of bovine herpesvirus 1 glycoprotein e antibodies in individual milk samples by enzyme-linked immunosorbent assays. | the purpose of this study was to determine whether individual milk samples can replace serum samples for the detection of bovine herpesvirus 1 (bhv1) glycoprotein e (ge)-specific antibodies. serum and milk samples were collected at the same time from cattle in bhv1-free herds, cattle in unvaccinated herds, and cattle in herds that were vaccinated twice with a bhv1 marker vaccine. the samples were tested in two ge enzyme-linked immunosorbent assay (elisa) systems. in comparison to serum, the resu ... | 1998 | 9466750 |
| characterization of the bovine herpesvirus 1 ul8 gene and gene products. | the bovine herpesvirus 1 (bhv-1) strain schönböken ul8 gene and the 5' flanking region were sequenced. comparison of the ul8 orf with the previously reported ul8 orf of bhv-1 strain cooper revealed significant differences that were mainly due to three frame-shifted segments. reanalysis of the cooper sequence after isolation of the respective segments from genomic dna by pcr did not confirm the discrepancies; on the contrary, our results indicate a high degree of sequence conservation between the ... | 1998 | 9460934 |
| fine mapping of bovine herpesvirus 1 (bhv-1) glycoprotein c neutralizing epitopes by type-specific monoclonal antibodies and synthetic peptides. | bovine herpesvirus glycoprotein c (gc) functions as a major virus attachment protein. here, two bhv-1 gc-specific epitopes that are specified by complement-dependent neutralizing mabs are mapped. the bhv-1 gc-specific peptides and mabs were used to specifically localize continuous epitopes by direct binding to the mabs and by blocking the mab reactivity (competitive elisa) to authentic viral antigen. the results of competitive elisa indicated that the complement-dependent neutralizing epitopes r ... | 1997 | 9453139 |
| the bovine herpesvirus type 1 ul3.5 open reading frame encodes a virion structural protein. | the bovine herpesvirus type 1 (bhv-1) open reading frame (orf) ul3.5 is similar to orfs found in pseudorabies virus, infectious laryngotracheitis virus, equine herpesvirus type 1, and varicella zoster virus, but clearly absent from herpes simplex virus. the published sequence for this orf predicts a 126-amino-acid (13.2 kda) protein product with an isoelectric point of 12.3. we confirmed the ul3.5 sequence, expressed the orf as a glutathione-s-transferase fusion protein, and made rabbit antibodi ... | 1998 | 9448691 |
| a comparison of different models for assessing variations in the sero-prevalence of infectious bovine rhinotracheitis by farm, area and district in kenya. | the relative variability of the sero-prevalence of antibodies to infectious bovine rhinotracheitis (ibr) due to cow, farm, and agroecological area levels were investigated for three contrasting districts in kenya: samburu, an arid and pastoral area; kiambu, a tropical highland area; and kilifi, a typical tropical coastal area. cattle were selected by two-stage cluster sampling and visited once between august 1991 and 1992. data on animal, farm, and area factors were analyzed using schall's algor ... | 1997 | 9443329 |
| sequencing of a 5.5-kb dna fragment and identification of a gene coding for a subunit of the helicase/primase complex of avian laryngotracheitis virus (iltv). | the nucleotide sequence of a 5,520-bp ecori restriction fragment of avian infectious laryngotracheitis (iltv) dna was reported and submitted to genebank with an accession number of af001078. computer prediction revealed one large potential open reading frame (orf) with sequence similar to one subunit of the dna helicase-primase complex of alpha-herpesviruses. the dna helicase/primase complex of hsv-1 consists of three sub-units with molecular weights of 12,000, 97,000 and 70,000, encoded by gene ... | 1997 | 9421876 |
| immunization of cattle with a bhv1 vector vaccine or a dna vaccine both coding for the g protein of brsv. | a ge-negative bovine herpesvirus 1 (bhv1) vector vaccine carrying a gene coding for the g protein of bovine respiratory syncytial virus (brsv) (bhv1/brsv-g) induced the same high degree of protection in calves against brsv infection and bhv1 infection as a multivalent commercial vaccine. a dna plasmid vaccine, carrying the same gene as the bhv1/brsv-g vaccine, significantly reduced brsv shedding after brsv infection compared with that in control calves, but less well than the bhv1/brsv-g vaccine ... | 1997 | 9413101 |
| serological evidence of bovine herpesviruses 1 and 2 in asian elephants. | antibodies were detected against bovine herpesviruses 1 (bhv 1) and 2 (bhv 2) in asian elephants (elephas maximus) using the passive hemagglutination (pha) test. the study was conducted during may to december 1994 using sera collected from zoological gardens and national parks in india. four (4%) of 109 elephant sera had pha titers ranging from 1:8 to 1:32 against bhv 1. twenty-five (23%) of the 109 elephant sera had pha titers ranging from 1:8 to 1:64 against bhv 2. asian elephants appear to be ... | 1997 | 9391986 |
| identification of dna sequences in the latency related promoter of bovine herpes virus type 1 which are bound by neuronal specific factors. | bovine herpes virus 1 establishes a latent infection in sensory ganglionic neurons of cattle. during a latent infection latency related (lr) transcripts are the only detectable viral rnas. dna sequences in the lr promoter are positively regulated by neural factors. the 5' terminus of lr rna in productively infected bovine cells is 20-30 nucleotides downstream from two overlapping tata like elements. in contrast, the major start sites of lr transcription in trigeminal ganglia of latently infected ... | 1997 | 9381799 |
| specific detection of shedding and latency of bovine herpesvirus 1 and 5 using a nested polymerase chain reaction. | a sensitive method for simultaneously detecting and discriminating between bovine herpesviruses types 1 and 5 (bhv-1 and bhv-5) was developed using a nested polymerase chain reaction (pcr) technique. following amplification using type-common primers derived from gc sequences, amplification using type-specific nesting primers produced different-sized bands specific to the corresponding types, as demonstrated by blot hybridization. less than 0.1 plaque-forming units (pfu) of each virus and 75 fg o ... | 1997 | 9376428 |
| infectious bovine rhinotracheitis, parainfluenza-3, and respiratory coronavirus. | a number of viruses have been proven to be primary respiratory pathogens of cattle. viruses may play an important role in making cattle susceptible to secondary respiratory bacterial pathogens. epidemiology, pathogenesis, laboratory diagnosis, and important properties in infectious bovine rhinotracheitis (ibr), parainfluenza-3 (pi-3), and bovine respiratory coronavirus (brcv) are described in this article. | 1997 | 9368989 |
| a molecular genetic approach to improved animal health. the effect of interferon genotype on the severity of experimental bovine herpesvirus-1 infection. | in the past decade, biotechnology has brought veterinary medicine an increased understanding of the effects of genetic background on disease resistance and production traits. specific point mutations have been identified for a number of genetic diseases and genetic testing; selective breeding programs can eliminate these diseases from the population. complex traits such as disease resistance and production traits are thought to be under the control of multiple genes, making their manipulation mo ... | 1997 | 9368986 |
| mucosal immunization with recombinant adenoviruses: induction of immunity and protection of cotton rats against respiratory bovine herpesvirus type 1 infection. | to facilitate the evaluation of vaccines against bovine herpesvirus type 1 (bhv-1), a cotton rat model of intranasal (i.n.) bhv-1 infection was established. cotton rat lung cells were similar to bovine cells in their ability to support bhv-1 replication in vitro. furthermore, i.n. inoculation of cotton rats with bhv-1 resulted in pulmonary lesions comparable to bhv-1 infection in cattle. using this model, the potential of i.n. and gastrointestinal (g.i.) immunization was examined with recombinan ... | 1997 | 9367381 |
| effect of il-4 and il-12 liposomal formulations on the induction of immune response to bovine herpesvirus type-1 glycoprotein d. | activation of different t-helper (th) responses following immunisation has profound and specific influences on the development of the immune response and on the ability of a vaccine to confer protection. since cytokines are capable of influencing the stimulation of distinct t-cell responses, their encapsulation in vaccines should modulate antigen-specific immune responses. unfortunately, the use of cytokines in vivo is hampered by their rapid clearance and inactivation. one possible solution to ... | 1997 | 9364679 |
| effect of bovine herpes virus-1, bluetongue virus and akabane virus on the in vitro development of bovine embryos. | bovine oocytes enclosed by follicular epithelial (fe) cells were exposed to bovine herpes virus-1 (bhv-1), bluetongue virus (btv) and akabane virus (av), matured in culture for 24 h and then in vitro fertilized. in the bhv-1-exposed group, bhv-1 was isolated from medium 3 days after in vitro fertilization at a high titer, and a severe cytopathic effect was observed in the fe cells. oocytes in the bhv-1-exposed group developed to the 2-cell to 8-cell stage, but failed to develop into blastocysts. ... | 1997 | 9355248 |
| induction of procoagulant activity in virus infected bovine alveolar macrophages and the effect of lipopolysaccharide. | three viruses known to be associated with the bovine respiratory disease complex were evaluated in vitro for potential impact upon the procoagulant activity (pca) of bovine alveolar macrophages (bam). cultures of bam were inoculated with bovine parainfluenza virus type 3 (pi-3), cytopathic bovine viral diarrhea virus (cpbvdv), non-cytopathic bvdv (ncpbvdv), or bovine herpes virus type 1 (bhv-1) and incubated for several time periods (24, 48, 72, 96 h). bam were then exposed to e. coli lipopolysa ... | 1997 | 9343337 |
| functional complementation of ul3.5-negative pseudorabies virus by the bovine herpesvirus 1 ul3.5 homolog. | the ul3.5 gene is positionally conserved but highly variable in size and sequence in different members of the alphaherpesvirinae and is absent from herpes simplex virus genomes. we have shown previously that the pseudorabies virus (prv) ul3.5 gene encodes a nonstructural protein which is required for secondary envelopment of intracytoplasmic virus particles in the trans-golgi region. in the absence of ul3.5 protein, naked nucleocapsids accumulate in the cytoplasm, release of infectious virions i ... | 1997 | 9343253 |
| antiviral activity of an extract from leaves of the tropical plant acanthospermum hispidum. | incubation of the alphaherpesviruses pseudorabiesvirus (prv) and bovine herpesvirus 1 during infection of cell cultures with an extract prepared from the leaves of acanthospermum hispidum impaired productive replication of these viruses in a concentration-dependent manner whereas propagation of classical swine fever virus, foot-and-mouth disease virus and vaccinia virus was not affected. the 50% inhibitory concentration for cell growth (ic50) was 107 +/- 5 microliters/ml, and the concentration r ... | 1997 | 9330761 |
| inactivated bovine herpesvirus 1 marker vaccines are more efficacious in reducing virus excretion after reactivation than a live marker vaccine. | a comparative study was carried out to evaluate the efficacy of three bovine herpesvirus 1 (bhv1) marker vaccines to reduce the reexcretion of virus after reactivation of latent bhv1. a live ge-negative vaccine, an inactivated ge-negative vaccine and an experimental gd-subunit vaccine were tested in three identical experiments in which cattle, latently infected with bhv1, were vaccinated twice before they were treated with high doses of dexamethasone. virus excretion after dexamethasone treatmen ... | 1997 | 9330461 |
| disease risks to animal health from artificial insemination with bovine semen. | two of the major goals of artificial insemination of domesticated animals are to achieve continuous genetic improvement and to prevent or eliminate venereal disease. in comparison with natural service, fewer males are needed to artificially inseminate the same number of females and to produce the same number of offspring. however, there are risks associated with artificial insemination, which has the potential to disseminate genetic defects and also to spread infectious disease nationally and in ... | 1997 | 9329119 |
| [serological investigations in the diagnosis of viral infections derived from cattle in sheep]. | in the coast region of the north-west of germany 269 arbitrary chosen blood samples of 22 flocks of sheep were tested for the existence of bhv-1, bvd and pi-3 antibodies. the livestock of 13 farms with 163 sheep consistent of sheep and bovine, 9 herds with 106 sheep kept sheep only. the results show that bhv-1 antibodies were not present in the sampled sheep. contrary to this are the results for bvd and pi-3. the blood tests for bvd antibodies were positive in 6.7% of the samples. this result in ... | 1997 | 9324461 |
| bovine herpesvirus-1 infects activated cd4+ lymphocytes. | acute virus infections can induce immune deficiencies, as shown by immunosuppression to a variety of antigens and mitogens. previously we observed that live bovine herpesvirus-1 (bhv-1) induced considerable lymphocyte death in culture, suggesting that the virus infected one or more cell populations. our goal was to identify the cells infected by bhv-1 and the mechanism resulting in cell death. cona activated cells were cultured with bhv-1 and stained with monoclonal antibodies specific for virus ... | 1997 | 9292002 |
| an enzyme-linked immunosorbent assay to detect antibodies against glycoprotein ge of bovine herpesvirus 1 allows differentiation between infected and vaccinated cattle. | a blocking enzyme-linked immunosorbent assay (elisa) was developed for detecting antibodies against glycoprotein ge (ge) of bovine herpesvirus 1 (bhv1). the assay is based on the use of two monoclonal antibodies directed against different antigenic domains on ge. sera from uninfected cattle and cattle that had been repeatedly vaccinated with ge-negative marker vaccines scored negative, whereas sera from cattle naturally or experimentally infected with bhv1 field strains scored positive in the ge ... | 1997 | 9274815 |
| study of immunogenicity and virulence of bovine herpesvirus 1 mutants deficient in the ul49 homolog, ul49.5 homolog and dutpase genes in cattle. | we previously reported that the bovine herpesvirus 1 (bhv 1) gene homologous to herpes simplex virus gene ul49 is dispensable; nevertheless, a mutant with the ul49 homolog (ul49 h) gene deletion exhibited significantly impaired growth in cell culture. to further evaluate the role of the ul49 h in virus infectivity in the natural host of bhv 1, the pathogenesis of the ul49 h negative mutant was studied in cattle. an additional mutant with a combined defect in ul49 h, ul49.5 h and dutpase genes wa ... | 1997 | 9269047 |
| analysis of bovine herpesvirus 1 transcripts during a primary infection of trigeminal ganglia of cattle. | during an infection of nonneuronal cells, bovine herpesvirus 1 (bhv-1) gene expression proceeds in a well-defined cascade. products of immediate-early (ie) genes are expressed first, and they activate expression of early (e) and late (l) genes. although the same cascade is assumed to occur during an infection of neurons in trigeminal ganglia (tg) of cattle, no experimental data is available to support this hypothesis. consequently, we analyzed bhv-1 gene expression in bovine tg at 1, 2, 4, 7, an ... | 1997 | 9261403 |
| polynucleotide vaccines in animals: enhancing and modulating responses. | we immunized cattle, the natural host for bovine herpesvirus 1 (bhv-1), with a polynucleotide vaccine encoding bhv-1 glycoprotein d. these cattle trials clearly indicate that large species can be immunized with polynucleotide vaccines. recently, using a murine model, we demonstrated that: the cellular compartment to which the expressed antigen is delivered determines the type of immune response (type 1 or type 2), and that the magnitude and direction of the immune response can be modulated by co ... | 1997 | 9234534 |
| assessing infections at multiple levels of aggregation. | the patterns of sero-prevalence of antibodies to four infectious diseases, representing a broad range of pathogens (bacteria: brucellosis; mycoplasma: contagious bovine pleuropneumonia; viruses: infectious bovine rhinotracheitis; protozoa: trypanosomosis) were investigated at three levels of organization (farm, area and district). three contrasting districts in kenya were compared: an arid and pastoral area (samburu); a tropical highland area (kiambu), and a tropical coastal area (kilifi). cattl ... | 1997 | 9234402 |
| the efficacy of trypsin for disinfection of in vitro fertilized bovine embryos exposed to bovine herpesvirus 1. | bovine cumulus-oocyte complexes (coc) or in vitro fertilized (ivf) embryos were exposed to bovine herpesvirus 1 (bhv-1) during in vitro maturation or co-culture with uterine tubal cells, respectively. trypsin, at a concentration of 0.25%, was applied (for approximately 90 s) to disinfect either coc or cumulus-free oocytes (cfo) 18 h after insemination, or on day 7 to embryos resulting from infected oocytes. in total, virus was not detected in 71% of 93 samples containing 233 embryos exposed to b ... | 1997 | 9233501 |
| herpesviral abortion in domestic animals. | abortion or neonatal disease may follow infection with several alpha, beta and gamma-herpesviruses. the alpha-herpesvirus, equid herpesvirus-1 (ehv-1), causes single or epizootic abortions or neonatal deaths in equids, and the closely related virus ehv-4 causes sporadic equine abortions. in cattle, the alpha-herpesviruses, bovine herpesvirus-1 (infectious bovine rhinotracheitis virus) and bovine herpesvirus-5 (bovine encephalitis virus), and a gamma-herpesvirus, bovine herpesvirus-4, have all be ... | 1997 | 9232116 |
| sheep do not have a major role in bovine herpesvirus 1 transmission. | with regard to bhv1 eradication programs in cattle it is important to know whether sheep can be a reservoir of bhv1. we therefore performed an experiment that consisted of three phases. in phase 1, 10 sheep were inoculated with high doses of bhv1 and kept in close contact with 5 sheep and 5 calves. all inoculated sheep excreted bhv1 between 8 and 15 days post inoculation and seroconverted. although bhv1 was isolated from the nasal mucosa of 3 out of 5 sentinel sheep, none of the sentinel sheep p ... | 1997 | 9231980 |
| [seroprevalence of viral infections in llamas (lama glama) in the republic of argentina]. | this study reports the seroprevalence of bovine viral infections in llamas (lama glama) in argentina. this is the first study made in the country including 390 llamas and testing antibodies against eight viruses. samples were collected from nine farms distributed in three different provinces: buenos aires, córdoba and jujuy. the samples were tested for antibodies against eight viruses known to infect cattle: bovine herpesvirus type 1 (bhv-1), bovine viral diarrhea virus (bvdv), bovine adenovirus ... | 1997 | 9229724 |
| the ge and gi homologs from two alphaherpesviruses have conserved and divergent neuroinvasive properties. | the membrane glycoproteins ge and gi are encoded by pseudorabies virus (prv), a neurotropic, broad-host-range alphaherpesvirus of swine. prv ge and gi are required for anterograde spread to a restricted set of retinorecipient neurons in the brain after infection of the rat retina. a related alphaherpesvirus, encoding ge and gi homologs, is called bovine herpesvirus 1.1 (bhv-1.1). bhv-1.1 is a respiratory pathogen of highly restricted host range and, in contrast to prv, is unable to propagate in ... | 1997 | 9223471 |
| effect of shipping and chromium supplementation on performance, immune response, and disease resistance of steers. | forty-eight angus crossbred steers (263 +/- 2 kg initial bw) were blocked by weight and randomly assigned within weight group to treatment. treatments consisted of control or .4 mg of supplemental cr as cr-nicotinic acid complex/kg of dm. steers were fed diets containing 90% corn silage (dm basis) and 10% of a soybean meal-mineral-vitamin supplement. after 56 d on the dietary treatment, half of the steers in each treatment were transported 343 km and unloaded in an unfamiliar location. the next ... | 1997 | 9222855 |
| [interactions between bronchial epithelial cells and extracellular matrix proteins]. | attachment and migration of bronchial epithelial cells are important in re-epithelialization after tissue injury. we hypothesized that inflammatory cytokines alter bronchial epithelial cell attachment and migration. to test this hypothesis, we studied the effects of mononuclear-cell-conditioned medium on attachment and migration of bronchial epithelial cells in response to fibronectin in vitro. this medium was prepared from bovine blood mononuclear cells that were stimulated with concanavalin a; ... | 1996 | 9216202 |
| interference activity of bovine herpes virus 1 strains against aujeszky's disease virus in cell cultures. | the interference-inducing activity of different low- and high-virulence strains of bovine herpes virus 1 (bhv-1) against aujeszky's disease virus was studied by a parallel titration on permissive and non-permissive cell culture. the low-virulence bhv-1 strains possessed better interference-inducing activity than the high-virulence strains. an interference blocking test (ibt) was developed for the detection of bhv-1 antibodies in bovine sera. comparative results of ibt and the virus neutralisatio ... | 1997 | 9208203 |
| infectious pustular vulvovaginitis/infectious pustular balanoposthitis in cattle. | 1997 | 9194305 | |
| identification and characterization of bovine herpesvirus-1 glycoproteins e and i. | to identify the products of the bovine herpesvirus-1 (bhv-1) ge and gi genes, we constructed baculovirus recombinants containing the putative ge or gi genes. these recombinant viruses synthesized bhv-1 ge and gi with apparent molecular masses of 84 and 41 kda, respectively. polyclonal antibodies against these recombinant ge or gi proteins were produced and by using these antibodies, we showed the presence of ge and gi with apparent molecular masses of 94 and 45 kda, respectively, in purified bhv ... | 1997 | 9191936 |
| bovine herpesvirus 1-induced apoptosis occurs at the g0/g1 phase of the cell cycle. | we have previously shown that bovine herpesvirus 1 (bhv-1), even when inactivated, induces apoptotic cell death in mitogen-stimulated bovine peripheral blood mononuclear cells (pbmcs) (hanon et al., 1996, j. virol. 70, 4116-4120). in order to gain insight into this process, we have investigated the cell cycle phase at which bhv-1 induces apoptosis in pbmcs. our results show that the percentage of cells that progress through the s phase was always lower in bhv-1-infected pbmcs than in control cel ... | 1997 | 9191849 |
| yeast-secreted bovine herpesvirus type 1 glycoprotein d has authentic conformational structure and immunogenicity. | bovine herpesvirus-1 (bhv-1) glycoprotein d (gd), an envelope glycoprotein, engenders mucosal and systemic immunity protecting cattle from viral infection. production of gd with authentic immunogenicity is required for a subunit vaccine. we placed the truncated bhv-1 gd gene, lacking its putative transmembrane and cytoplasmic domains, under the control of the methanol-inducible aox1 promoter in the yeast pichia pastoris. truncated bhv-1 gd (tgd) was efficiently secreted into the culture medium a ... | 1997 | 9178470 |
| restriction endonuclease analysis of dna from indian isolates of bovine herpesvirus 1. | the genomic variation of three isolates of bovine herpes virus 1 (bhv-1) originating from different geographical regions and displaying different clinical symptoms were studied by restriction analysis using four different restriction endonucleases. ecori displayed an uniform restriction pattern for all the isolates and hindiii showed marginal variation among the isolates. bsteii and psti displayed unique restriction patterns, based on which the isolates could be grouped into two subgroups of bhv ... | 1996 | 9171462 |
| necrotic oophoritis in gilts associated with experimental inoculation of a viral gene-deletion mutant pseudorabies vaccine. | previous work on the reproductive effects of various herpesviruses has demonstrated adverse effects on reproductive function in several host species. although herpesviral vaccines are used in several species to ameliorate the clinical effects of infection, pathogenicity for reproductive tissue, associated with diminished reproductive efficiency, has been reported to be retained in a live-attenuated vaccine strain of the herpesvirus, bovine herpesvirus-1. the objective of this study was to determ ... | 1997 | 9163875 |
| sequence analysis of the bovine herpesvirus type 1 genes homologous to the dna polymerase (ul30), the major dna-binding protein (ul29) and icp18.5 assembly protein (ul28) genes of herpes simplex virus. | the nucleotide sequence of a 10.5 kb region (map position 0.332 to 0.410) of bovine herpesvirus type 1 (bhv-1) was determined. this region contained three open reading frames (orfs) homologous to herpes simplex virus dna polymerase catalytic subunit (dnapol, ul30), major dna-binding protein (mdbp, ul29) and icp18.5 assembly protein (icp18.5, ul28). the bhv-1 dnapol. mdbp and icp18.5 orfs were 1246, 1203 and 826 amino acids long with a calculated molecular mass of 134.2 kda, 124.4 kda and 86.9 kd ... | 1997 | 9155875 |
| bovine herpesvirus 1 glycoprotein b does not productively interact with cell surface heparan sulfate in a pseudorabies virion background. | attachment to cell surface heparan sulfate proteoglycans is the first step in infection by several alphaherpesviruses. this interaction is primarily mediated by virion glycoprotein c (gc). in herpes simplex virus, in the absence of the nonessential gc, heparan sulfate binding is effected by glycoprotein b. in contrast, gc-negative pseudorabies virus (prv) infects target cells via a heparan sulfate-independent mechanism, indicating that prv virion gb does not productively interact with heparan su ... | 1997 | 9151882 |
| nucleotide sequence and transcriptional analysis of a portion of the bovine herpesvirus genome encoding genes homologous to hsv-1, ul25, ul26 and ul26.5. | we determined the nucleotide sequence of a 3,811 base pair (bp) region of the bovine herpesvirus type-1 (bhv-1) hindiii-a fragment covering map units 0.429-0.456, which contained the hsv-1 homologues of the ul25, ul26 and ul26.5 genes. sequence overlap was verified with previously published bhv-1 sequences, resulting in contiguous sequence spanning from ul24 to ul27 (encoding glycoprotein b). we found that the ul25 open reading frame (orf) overlapped that of ul24 by 16 nucleotides, an unexpected ... | 1997 | 9140190 |
| serologic survey for infectious pathogens in free-ranging american bison. | from november 1991 through march 1992, we evaluated 101 free-ranging american bison (bison bison) from yellowstone national park, wyoming (usa) for exposure to infectious organisms that commonly infect cattle. no titers were detected for bluetongue virus, bovine leukemia virus, or campylobacter fetus in these 101 bison. detectable antibodies occurred against anaplasma marginale (eight of 76, 11%), bovine respiratory syncytial virus (31 of 101, 31%), bovine viral diarrhea (31 of 101, 31%), bovine ... | 1997 | 9131564 |