Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| transgenic rice cell lines and plants: expression of transferred chimeric genes. | cell suspension-derived rice (oryza sativa l.) protoplasts were transformed by direct gene uptake. peg-mediated transformation was more efficient than electroporation. plasmid dna containing a hygromycin phosphotransferase (hpt) gene (which confers hygromycin resistance) driven by the camv 35s promoter and a beta-d-glucuronidase (gus) gene under control of the 1', 2' double promoter of the mannopine synthase (mas) locus of agrobacterium tumefaciens was introduced into rice protoplasts. southern ... | 1991 | 1713479 |
| diversification of the rice waxy gene by insertion of mobile dna elements into introns. | the waxy (wx) gene of oryza glaberrima was cloned, and its nucleotide sequence was determined. a waxy mutant of o. glaberrima showing a glutinous phenotype was found to contain a substitution mutation generating a termination codon in the coding region of the wx gene. the wx sequence of o. glaberrima was different from that of oryza sativa by substitutions and insertions/deletions, among which only a few substitutions occurred in several exons not to severely alter the amino acid sequence of the ... | 1991 | 1685658 |
| the isolation and characterisation of plant sequences homologous to human hypervariable minisatellites. | we have isolated dna probes, homologous to the human hypervariable minisatellite sequence 33.15, from the genome of rice (oryza sativa). these probes are capable of producing a multilocus rice dna fingerprint. the rice sequence has a tandem repeating structure based on a 12 bp gc-rich repeat which shows homology to its human counterpart. this probe detects up to 30 loci which are at a number of unlinked chromosomal sites. the gc-rich sequence is invariably associated with an open reading frame ( ... | 1991 | 1651259 |
| introduction and transposition of the maize transposable element ac in rice (oryza sativa l.). | to develop a transposon tagging system in an important cereal plant, rice (oryza sativa l.), the maize transposable element ac (activator) was introduced into rice protoplasts by electroporation. we employed a phenotypic assay for excision of ac from the selectable hph gene encoding resistance to hygromycin b. southern blot analysis of hygromycin b-resistant calli showed that the ac element can transpose from the introduced hph gene into the rice chromosomes. sequence analysis of several ac exci ... | 1991 | 1650907 |
| isolation and molecular evolutionary analysis of a cytochrome c gene from oryza sativa (rice) | a cytochrome c gene, oscc-1, from rice (oryza sativa) has been isolated and analyzed. the oscc-1 gene encodes a cytochrome c protein that is typical of higher-plant cytochrome c proteins. oscc-1 consists of three exons separated by two introns that are 817 and 747 bp in length, respectively. from genomic dna hybridization analysis, oscc-1 appears to be one of possibly two cytochrome c genes in several asian, american, and indian rice species and varieties surveyed. a single, unique cytochrome c ... | 1991 | 1646373 |
| characterization of cis-acting elements regulating transcription from the promoter of a constitutively active rice actin gene. | the promoter of the constitutively expressed rice (oryza sativa) actin 1 gene (act1) is highly active in transformed rice plants (w. zhang, d. mcelroy, and r. wu, plant cell 3:1150-1160, 1991). a region 834 bp upstream of the act1 transcription initiation site contains all the regulatory elements necessary for maximal gene expression in transformed rice protoplasts (d. mcelroy, w. zhang, j. cao, and r. wu, plant cell 2:163-171, 1990). we have constructed a series of act1 promoter deletions fused ... | 1992 | 1630454 |
| nucleotide sequence of the rice (oryza sativa) em protein gene (emp1). | 1992 | 1623185 | |
| nucleotide sequences of two cdna clones encoding different cu/zn-superoxide dismutases expressed in developing rice seed (oryza sativa l.). | 1992 | 1623183 | |
| co-transcription of orf25 and coxiii in rice mitochondria. | southern hybridization analysis using homologous maize probes indicated that orf25 and coxiii are closely linked in the mitochondrial genome of rice (oryza sativa) cultivar ir36. the two coding regions were found on the same 5.1 kb bamhi fragment, and this fragment was cloned, mapped and partially sequenced. using probes for each gene derived from the rice clone, a 2.4 kb dicistronic mrna transcript was found containing both orf25 and coxiii coding regions. multiple 5' ends were identified by pr ... | 1992 | 1617739 |
| two rice (oryza sativa) full-length cdna clones encoding low-molecular-weight heat-shock proteins. | 1992 | 1581572 | |
| genomic structure of the gene for copper/zinc-superoxide dismutase in rice. | we have isolated and determined the nucleotide sequence of the structural gene (sodb) coding for one of the copper/zinc-superoxide dismutase (cu/zn-sod) isozymes from rice plants (oryza sativa l.). the sodb gene is split into 8 exons spread about 2 kb of the chromosomal dna, with the coding sequence beginning in the 2nd and ending in the 8th. although none of sodb introns are inserted into similar positions in cu/zn-sod genes from other sources, four of six introns in the protein-coding region a ... | 1992 | 1568478 |
| surface structure and properties of plant seed oil bodies. | storage triacylglycerols (tag) in plant seeds are present in small discrete intracellular organelles called oil bodies. an oil body has a matrix of tag, which is surrounded by phospholipids (pl) and alkaline proteins, termed oleosins. oil bodies isolated from mature maize (zea mays) embryos maintained their discreteness, but coalesced after treatment with trypsin but not with phospholipase a2 or c. phospholipase a2 or c exerted its activity on oil bodies only after the exposed portion of oleosin ... | 1992 | 1560029 |
| the hydroxyproline-rich glycoprotein gene from oryza sativa. | 1992 | 1536936 | |
| presence of three rice sucrose synthase genes as revealed by cloning and sequencing of cdna. | by sequencing cdna clones, we have concluded that three distinct sucrose genes are expressed in rice (oryza sativa cv. tainong 67). when the amino acid sequences deduced from these cdnas as well as those of known sucrose synthase are compared, the highest divergence is found in the c-termini. the most suitable dna sequences for use as specific for the mrna derived from these genes have been suggested. | 1992 | 1534703 |
| the amino acid sequence of a 20 kda bifunctional subtilisin/alpha-amylase inhibitor from bran [correction of brain] of rice (oryza sativa l.) seeds. | a 20 kda bifunctional inhibitor of the microbial proteinase, subtilisin, and the alpha-amylase from the larvae of the red flour beetle (tribolium castaneum) was purified from bran of rice seeds by saline extraction, precipitation with ammonium sulphate, ion-exchange chromatography on deae-cellulose and toyopearl cm-650, and preparative hplc on vydac c18. the complete primary structure was determined by automatic degradation of the intact, reduced and s-alkylated protein, and by manual dabitc/pit ... | 1992 | 1511747 |
| isolation of genes abundantly expressed in rice anthers at the microspore stage. | a cdna library of rice (oryza sativa l.) has been constructed from anthers at an early stage of pollen development. by differential screening of the library, we have isolated cdnas of two genes, designated as osc4 and osc6, that are abundantly expressed in anthers containing tetrads and uninucleate microspores, but are not expressed in leaves or roots. expression of osc4 is absent in mature anthers, while osc6 is present although the expression decays during pollen maturation. a comparison of th ... | 1992 | 1463854 |
| factors influencing agrobacterium-mediated transient expression of gusa in rice. | transient expression of gus in rice (oryza sativa l.) mediated by agrobacterium tumefaciens was characterized using binary vectors containing gusa genes that express minimal (pkiwi105 and pcnl1) or no (p35s-gus-int and pcnl56) gus activity in bacteria. four-day old seedlings obtained from seeds or immature embryos of rice were cut into shoot, root, and seed remnants and inoculated with various strains of a. tumefaciens. transient gus expression events were quantitated histochemically by determin ... | 1992 | 1463839 |
| the wheat mitochondrial genome contains an orf showing sequence homology to the gene encoding the subunit 6 of the nadh-ubiquinone oxidoreductase. | a region of the mitochondrial (mt) dna of wheat was studied because of its homology with other plant mtdnas. sequence analysis revealed an open reading frame encoding a polypeptide of 247 amino acids. comparison of the sequence of the putative polypeptide with the protein sequence data of the swiss-prot library reveals homology with subunit 6 of the nadh-ubiquinone complex of mitochondria from marchantia polymorpha, podospora anserina, chlamydomonas reinhardtii and of chloroplasts from m. polymo ... | 1992 | 1421143 |
| efficiency of the 5'-terminal sequence (omega) of tobacco mosaic virus rna for the initiation of eukaryotic gene translation in escherichia coli. | recent studies have demonstrated that the 5' leader (omega sequence) of tobacco mosaic virus rna has a certain enhancing capacity for translation of mrna in both prokaryotes and eukaryotes. in order to estimate the efficiency of omega to initiate translation of mrna in escherichia coli, in comparison to the shine-dalgarno (s/d) sequence, we have inserted eight different eukaryotic genes into two types of e. coli expression vectors containing one constitutive promoter (p1) but different translati ... | 1992 | 1396694 |
| nucleotide sequence of a cdna encoding a beta subunit of the mitochondrial atpase from rice (oryza sativa). | 1992 | 1387558 | |
| isolation and characterization of cdna clones encoding cdc2 homologues from oryza sativa: a functional homologue and cognate variants. | using probes obtained by pcr amplification, we have isolated two cognate rice cdnas (cdc2os-1 and cdc2os-2) encoding structural homologues of the cdc2+/cdc28 (cdc2) protein kinase from a cdna library prepared from cultured rice cells. comparison of the deduced amino acid sequences of cdc2os-1 and cdc2os-2 showed that they are 83% identical. they are 62% identical to cdc28 of saccharomyces cerevisiae and much more similar to the yeast and mammalian p34cdc2 kinases than to rice r2, a cdc2-related ... | 1992 | 1376401 |
| purification and characterization of rice peroxidases. | four peroxidase components, named rp-2, 4, 6, and 7, were isolated from rice (oryza sativa l.) green leaves. isoelectric focusing indicated that each preparation was homogeneous. the molecular weights of rp-2, 4, 6, and 7 estimated by sds-page were 48,000, 48,000, 40,000, and 39,500, and their isoelectric points were 5.4, 8.1, 9.3, and 9.2, respectively. the activity of every preparation was maximum around ph 5.0. antisera against these purified enzymes were raised in rabbits. ouchterlony double ... | 1991 | 1368754 |
| molecular cloning and characterization of gravity specific cdna in rice (oryza sativa l.) suspension callus. | rice (oryza sativa l. var. nipponbare) suspension callus was exposed to gravity stress at 450,000 g for 2 hours, after which poly(a)+rna was isolated and a cdna library was constructed. three different gravity specific cdnas, namely, gsc 128, gsc 233 and gsc 381 of 0.67, 0.60 and 0.68 kilobase pairs and transcripts of 1.9, 1.6 and 2.0 kb, respectively, were isolated by differential screening and northern hybridization. the maximum level of transcript was achieved after 4 hours of exposure to gra ... | 1992 | 1363521 |
| genetic and physical analysis of the rice bacterial blight disease resistance locus, xa21. | nearly isogenic lines (nils) of rice (oryza sativa) differing at a locus conferring resistance to the pathogen xanthomonas oryzae pv. oryzae were surveyed with 123 dna markers and 985 random primers using restriction fragment length plymorphism (rflp) and random amplified polymorphic dna (rapd) analysis. one chromosome 11 marker (rg103) detected polymorphism between the nils that cosegregated with xa21. all other chromosome 11 dna markers tested were monomorphic between the nils, localizing the ... | 1992 | 1362973 |
| chromosome location of oryza sativa recombination linkage groups. | in situ hybridization, a powerful tool for the molecular cytogeneticist, can be used to physically map repetitive, low-copy, and unique dna sequences in plant chromosomes. with the availability of a recombination map in oryza sativa l. and an improved in situ hybridization technique, this study was designed to establish the relationship between the genetic and physical distances of the rice restriction fragment length polymorphism map. analysis indicated that considerable variation can exist bet ... | 1992 | 1356266 |
| characterization and genetic mapping of a short, highly repeated, interspersed dna sequence from rice (oryza sativa l.). | a short, highly repeated, interspersed dna sequence from rice was characterized using a combination of techniques and genetically mapped to rice chromosomes by restriction fragment length polymorphism (rflp) analysis. a consensus sequence (ggc)n, where n varies from 13-16, for the repeated sequence family was deduced from sequence analysis. southern blot analysis, restriction mapping of repeat element-containing genomic clones, and dna sequence analysis indicated that the repeated sequence is in ... | 1992 | 1347144 |
| herbicide-resistant indica rice plants from irri breeding line ir72 after peg-mediated transformation of protoplasts. | the commercially important indica rice cultivar oryza sativa cv. ir72 has been transformed using direct gene transfer to protoplasts. peg-mediated transformation was done with two plasmid constructs containing either a camv 35s promoter/hph chimaeric gene conferring resistance to hygromycin (hg) or a camv 35s promoter/bar chimaeric gene conferring resistance to a commercial herbicide (basta) containing phosphinothricin (ppt). we have obtained so far 92 hgr and 170 pptr ir72 plants from protoplas ... | 1992 | 1333295 |
| characterization of a plant sine, p-sine1, in rice genomes. | we have previously found that a short interspersed element (sine), named p-sine1, is present in the waxy gene of oryza sativa in two copies. here, we cloned five members of p-sine1 located at other loci in o. sativa and determined their nucleotide sequences. these sequences had a t-rich pyrimidine tract at their defined 3' end and were flanked by direct repeats of a sequence of mostly 14-15 bp long like p-sine1s in the waxy gene. the consensus sequence derived from total seven members of p-sine1 ... | 1992 | 1326298 |
| molecular characterization of cdna encoding for adenylate kinase of rice (oryza sativa l.). | two types of genes (adk-a, and adk-b) encoding for adenylate kinase (ak, ec 2.7.4.3.) were isolated from the cdna library constructed from poly(a)+ rna of rice (oryza sativa l.). two cdnas were heterogeneous at 5' and 3' ends of non-coding sequences and had possible polyadenylation signals. one of the genes, adk-a, had 1154 bp sequences encoding 241 amino acid residues, while the other type, adk-b, contained 1085 bp sequences encoding for 243 amino acid residues. homology between adk-a and adk-b ... | 1992 | 1302636 |
| the limit dextrinases from ungerminated oats (avena sativa l.) and ungerminated rice (oryza sativa l.). | the limit dextrinases from ungerminated oats and rice have been purified, and their substrate specificity compared with a bacterial isoamylase preparation. both cereal enzymes could hydrolyse (1 yields6)-alpha-d-glucosidic linkages in oligosaccharide alpha-dextrins, pullulan, amylopectin, and the beta-limit dextrins of amylopectin and glycogen. however, under comparable conditions, they were unable to attack glycogens. | 1975 | 1170015 |
| reversible redistribution of phytochrome within the cell upon conversion to its physiologically active form. | the intracellular localization of phytochrome was seen in dark-grown oat (avena sativa l., cv. garry) and rice (oryza sativa l., cv. unknown) shoots after various light treatments using an indirect peroxidase-antiperoxidase antibody labeling method. phytochrome is generally distributed throughout the cytoplasm in cells of tissue that had not been exposed to light prior to fixation. within, at most, 8 min after the onset of saturating red irradiation, phytochrome, now present in the far-red-absor ... | 1975 | 1093170 |
| quantitative genetically nonequivalent reciprocal crosses in cultivated plants. | quantitative expressions of character difference between reciprocal crosses have been studied by different researchers in a number of plant species, such as epilobium, zea mays, oryza sativa, hordeum sativum, triticum aestivum, trifolium hybridum, linum usitatissimum, nicotiana rustica, and others. in all cases it was found that the nonequivalence of reciprocal crosses manifested itself beginning with the f1 generation, with the exception of some flax crosses in which reciprocals differed beginn ... | 2015 | 1032105 |
| volatile components of unprocessed rice (oryza sativa l.). | 1976 | 1002944 | |
| hydrogen isotope effects in the cyclization of d-glucose 6-phosphate by myo-inositol-1-phosphate synthase. | myo-inositol-1-phosphate synthase (ec 5.5.1.4) from rat testis, acer pseudoplatanus l. cell culture and oryza sativa l. cell culture, converted d-[5-3h]glucose 6-phosphate to myo-[2-3h]inositol 1-phosphate at rates ranging from 0.21 to 0.48 that of unlabeled substrate. d-[3-3h]- and d-[4-3h]glucose 6-phosphate were converted at approximately the same rate as that of unlabeled substrate. in the case of testis enzyme, storage as a frozen solution further lowered the rate with d-[5-3h]glucose 6-pho ... | 1977 | 903359 |
| in vitro development of plants from microspores of rice. | rice (oryza sativa l., 2n=24) anthers containing microspores in the early-uninucleate to first-mitosis stages were induced successfully to develop into plants in vitro through an intermediary step of callus formation. callus initiation occurred with highest frequency in anthers containing mid-uninucleate imcrospores. the callus derived from different stages of microspore development differed in the potential to differentiate into plants. the plants regenerated from pollen callus were predominant ... | 1977 | 903172 |
| mutagenic effect of argemone oil in oryza sativa l. | in view of the large scale adulteration of edible oils with argemone oil, a study was undertaken to test its mutagenicity on rice seed. in treatment with screw-pressed oil a marked reduction in seed germination and high chlorophyll mutation and mutant frequencies were observed. in sequential treatments between gamma rays and argemone oil both mutation and mutant frequencies were significantly higher than that of individual treatments. this study clearly indicates that argemone oil is a potent mu ... | 1977 | 876267 |
| factors modifying radiation induced stimulation in plants: pre-irradiation seed moisture content. | the effect of moisture content of seeds at the time of irradiation in relation to radiation-induced stimulation was investigated on rice (oryza sativa c.v. d-6-2-2). the optimum moisture content was 8% for stimulation measured as seedling height. it is concluded that seed moisture at the time of irradiation plays an important role in the expression of stimulation and its reproducibility. | 1977 | 866584 |
| light-induced development of photosynthetic enzymes in etiolated detached leaves of oryza sativa and setaria italica during greening. | 1977 | 612539 | |
| production of 9-beta-d-arabinofuranosyladenine by a new species of streptomyces and its herbicidal activity. | 9-beta-d-arabinofuranosyladenine (ara-a) was produced by a new species of streptomyces designated as s. herbaceus. ara-a was found to have potent herbicidal activity against echinochloa crus-galli, digitaria adscendens and chenopodium ficifolium by the treatment with ara-a before germination of these plants. however, oryza sativa had strong resistance to ara-a. | 1979 | 528365 |
| [bean and rice mixtures of high protein value (author's transl)]. | the optimum content of different rice (oryza sativa) and bean (vigna unguiculata l.) combinations was studied in albino rats. the mixture containing 3 g of rice proteins, corresponding to 41g of rice "in natura", and 7 g of bean protein, corresponding to 59 g of bean "in natura", presented the highest protein efficiency ratio and the feed efficiency ratio. since the limiting amino acid of this mixture was methionine, new assays using varying levels of this amino acid as a supplement were carried ... | 1979 | 515465 |
| purification and characterization of a trypsin inhibitor from rice bran. | a trypsin inhibitor was isolated and purified from the bran of rice, oryza sativa, by extraction with 1% sodium chloride, heat treatment, ammonium sulfate precipitation, ion-exchange chromatography on a cm-sephadex c-25 and gel filtration on a sephadex g-75. the final preparation was homogeneous by electrophoretic analysis. rice bran trypsin inhibitor (rbti) had a molecular weight of about 14,500 and an isoelectric point of 8.07. the amino acids, acid composition was characterized by high conten ... | 1979 | 501453 |
| herbimycin, a new antibiotic produced by a strain of streptomyces. | herbimycin, a new antibiotic, was isolated from the fermentation broth of streptomyces hygroscopicus strain no. am-3672, a soil isolate. the molecular formula of herbimycin was determined to be c30h42n2o9. herbimycin was found to have potent herbicidal activity against most mono- and di-cotyledonous plants, especially against cyperus microiria steud. however, oryza sativa showed strong resistance to herbimycin. | 1979 | 468713 |
| protein quality in cereals and pulses. 1. application of microbiological and other in vitro methods in the evaluation of rice (oryza sativa l.), sorghum (sorghum vulgare pers.), barley and field beans (vicia faba l.). | 1. the streptococcus zymogenes assay procedure was modified and used in the estimation of available methionine and relative nutritional value in rice (oryza sativa l.), sorghum (sorghum vulgare pers.), barley and field beans (vicia faba l.). the results were assessed in relation to the content of tannins and 'dye-binding lysine', and to published information on the nutritional quality of some of the test samples. 2. in grain of ten varieties of sorghum, for which other workers had reported a cor ... | 1979 | 427087 |
| penicillin-induced formation of ribonuclease in rice (oryza sativa l.) endosperm and its inhibition by abscisic acid. | penicillin stimulates the formation of ribonuclease in embryoless rice (oryza sativa l.) endosperm and enhances gibberelin-induced response. penicillin-induced rnase production is completely inhibited by abscisic acid. | 1979 | 227713 |
| the reaction of mitochondria in the coleoptiles of rice (oryza sativa l.) with diaminobenzidine. | diaminobenzidine, dab, was applied to segments of aerobically and anaerobically grown coleoptiles of rice, oryza sativa l., with the object of studying the location of cytochrome oxidase at the electron-microscope level. a specific staining of mitochondrial cristae and inner membrane was obtained, with no reaction in other organelles; with extended periods of incubation, the reaction product filled the mitochondria completely. in anaerobically grown coleoptiles, the reaction was much slower and ... | 1993 | 46231 |