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hemorrhagic bullae in association with enterobacter cloacae septicemia. 19921401325
characterization of the plasmid mediated beta-lactamase bil-1.a multi-resistant strain of escherichia coli isolated from a patient from pakistan was shown to possess a new plasmid-mediated beta-lactamase. this enzyme, designated bil-1, conferred resistance to extended-spectrum cephalosporins such as cefotaxime and ceftazidime. however, unlike other plasmid-mediated beta-lactamases capable of conferring resistance to these drugs, the bil-1 was not a member of the tem or shv group of plasmid-mediated beta-lactamases and it also conferred resistance to beta-l ...19921399921
cefclidin (e1040), a novel cephalosporin: lack of selection of beta-lactamase overproducing mutants in an in vitro pharmacokinetic model system.the bactericidal activity of cefclidin (e1040), a new cephalosporin, against a clinical strain of citrobacter freundii was compared with that of ceftazidime in a two-compartment in vitro pharmacokinetic model system designed to simulate plasma concentrations in humans for 12 hours after intravenous administration of a 1 g dose. both cefclidin and ceftazidime showed rapid bactericidal activity against c. freundii. however, during the simulation of ceftazidime treatment, regrowth was observed afte ...19921399855
postoperative posterior spinal wound infections.the incidence of postoperative spinal infections increases with the complexity of the procedure. diskectomy is associated with less than a 1% risk of infection; spinal fusion without instrumentation is associated with a 1%-5% risk; and fusion with instrumentation may be associated with a risk of 6% or more. twenty-two postoperative posterior spinal infections that occurred during a three-year period were reviewed for this report. staphylococcus aureus was the most frequent organism cultured (mor ...19921395319
sheen formation and growth response of groundwater bacteria to reduced oxygen concentrations during incubation of m-endo medium.in vitro pure-culture studies were conducted to assess growth and sheen formation of groundwater bacteria on m-endo medium incubated under reduced oxygen concentrations (0, 4, 8, 12, and 16%). coliform and noncoliform bacteria were isolated from 17 untreated, rural groundwater supplies on m-endo medium. all 16 coliform isolates tested were capable of sheen formation at oxygen concentrations of 4% or greater, yet some of these same isolates (enterobacter aerogenes, enterobacter cloacae, and hafni ...19921393829
application and assessment of cloacin typing of enterobacter cloacae.three methods, o-serotyping, phage typing and susceptibility to bacteriocins, were used to type 357 clinical isolates of enterobacter cloacae cultured from 219 patients. one hundred and sixty isolates were typed by serology and phage typing. when these two methods were used, primary classification of isolates was based on serology (65.7% typable) and phage typing for further subdivision (94.1% typable). when all the isolates were typed by cloacin susceptibility, 81.5% of them were typable. maxim ...19921373422
infections during a randomized trial comparing cyclosporine to fk 506 immunosuppression in liver transplantation. 19921371626
amino acid substitutions in naturally occurring variants of ail result in altered invasion activity.yersinia enterocolitica is the causative agent of a variety of gastrointestinal syndromes ranging from acute enteritis to mesenteric lymphadenitis. in addition, systemic infections resulting in high mortality rates can occur in elderly and immunocompromised patients. more than 50 serotypes of y. enterocolitica have been identified, but only a few of them commonly cause disease in otherwise healthy hosts. those serotypes that cause disease have been divided into two groups, american and non-ameri ...19921370953
occurrence of aminoglycoside phosphotransferase subclass i and ii structural genes among enterobacteriaceae spp. isolated from meat samples.3'-aminoglycoside phosphotransferase [aph(3')] enzymes are a group responsible for resistance to the antibiotics kanamycin (km) and neomycin (nm) in bacteria. escherichia coli ect24, originally isolated from a meat sample, harboured an 83-kb conjugative r-plasmid (prpj24) that carries transferable resistance to km and nm. plasmid prpj24 was transferred by conjugation to enterobacter cloacae 94r, which was used as the source of plasmid dna in development of a probe for the km-resistance determina ...19901369284
coupled reductive and oxidative degradation of 4-chloro-2-nitrophenol by a co-immobilized mixed culture system.the restriction of oxygen transfer in ca-alginate beads used for the immobilization of microbial cells was applied to a coupled reductive and oxidative microbial degradation of the xenobiotic 4-chloro-2-nitrophenol (cnp). the conversion of cnp by enterobacter cloacae under anaerobic conditions led to the formation of 4-chloro-2-aminophenol (cap, 81%) and 4-chloro-2-acetaminophenol (caap, 16%) after 50 h incubation. cap, the main reduction product, was further degraded under aerobic conditions by ...19901366971
a method for removal of toxic chromium using dialysis-sac cultures of a chromate-reducing strain of enterobacter cloacae.a method for removal of toxic hexavalent chromium (chromate: cro2-4) was developed by use of dialysis-sac cultures of a chromate-reducing strain of enterobacter cloacae (ho1). e. cloacae strain ho1 cells were put in dialysis (semipermeable membrane) sacs, and the sacs were submerged in water containing toxic cro2-4. the dialysis sacs allowed cro2-4 to diffuse into the culture, and cro2-4 was reduced anaerobically in the dialysis sacs by the e. cloacae cells. because reduced chromium readily form ...19901366562
[panophthalmitis as a nosocomial infection].the submitted paper deals with an epidemic of severe postoperative panophthalmitis, its development, course, causes and sequelae incl. epidemiological characteristics. the disease developed in four patients 40 hours after operation of cataract. despite treatment all four patients developed septicaemia and therefore the affected eyeballs were eviscerated. from smears of the conjunctival sac of the affected patients and from the contents of the eviscerated eyeball proteus mirabilis and enterobacte ...19911364655
characterization of the chromosomal aac(6')-ic gene from serratia marcescens.the dna sequence of the chromosomal aac(6')-ic gene from serratia marcescens, which had been previously cloned (h. m. champion, p. m. bennett, d. a. lewis, and d. s. reeves, j. antimicrob. chemother. 22:587-596, 1988) was determined. high-pressure liquid chromatographic analysis of extracts prepared from escherichia coli carrying the chromosomal aac(6')-ic gene on a plasmid confirmed the presence of 6'-n-acetyltransferase activity in this strain, which was suggested by the aminoglycoside resista ...19921354954
rapid genotyping shows the absence of cross-contamination in enterobacter cloacae nosocomial infections.restriction fragment length polymorphism analysis (rflp) of total dna and rdna regions was used for the epidemiological evaluation of 10 enterobacter cloacae nosocomial isolates obtained from nine patients in our hospital. five of these patients were hospitalized during overlapping periods, thus raising the question of cross-contamination. a single biochemical pattern and antibiotic susceptibility profile was observed for all isolates but one. in contrast, based on the results of total dna and r ...19921353097
molecular analysis provides evidence for the endogenous origin of bacteremia and meningitis due to enterobacter cloacae in an infant.we analyzed the restriction fragment length polymorphism (rflp) of total dna and of ribosomal dna regions (ribotyping) to document the occurrence of endogenous, systemic bacteremia and meningitis due to enterobacter cloacae in a newborn. five strains of e. cloacae were isolated from this newborn. three of these strains were recovered from stool at counts of 10(8), 10(9), and 10(9) organisms/g of feces, respectively; one strain was isolated from blood; and one strain was isolated from cerebrospin ...19921352150
handwashing and disinfection of heavily contaminated hands--effective or ineffective?hands are among the principal vehicles for transfer of nosocomial pathogens in hospitals. often, outbreaks of infection are thought to be caused by a lack of compliance with handwashing guidelines, rather than due to the inadequacy of the handwashing agents used. in this study the effectiveness of proper handwashing and the use of three different hand disinfectants: ethanol 70% (e), isopropanol 40% (i) and alcoholic chlorhexidine (70%) (ac) was compared using three volunteers whose fingertips we ...19921351497
epidemiological role of arthropods detectable in health facilities.a total of 161 arthropod specimens were collected from 55 sites in a health care facility during july and september 1990. of the 116 bacterial isolates obtained from their body surfaces 6% were from parasites (mosquitoes), 59% from eusynanthropic arthropods (tenebrionid beetles, flies, german cockroaches, wasps), 16% from hemisynanthropic arthropods (ants, spiders) and 19% from occasionally encountered insects (non-biting midges, moths, beetles). most (88%) of the isolated bacteria were gram-neg ...19921350604
sepsis associated with transhepatic cholangiography.a retrospective study was carried out of 74 elderly patients with obstructive jaundice undergoing percutaneous transhepatic cholangiography (ptc) and/or percutaneous biliary drainage (pbd) in order to assess the effect of prophylactic antibiotics on the incidence of fever and sepsis complicating these procedures. seventeen patients underwent ptc alone, while 57 had both ptc and pbd. fifty-three patients had either primary or metastatic malignancy. in the other patients with benign disease, chole ...19921348071
retrospective 6-year study of enterobacter bacteraemia in a danish university hospital.in order to study the epidemiology of invasive enterobacter infections, data from 53 consecutive cases of bacteraemia due to this organism were compared with data from 72 randomly selected cases of escherichia coli bacteraemia. the cases occurred among patients admitted to a danish university hospital over a 6-year period. forty-eight cases were due to enterobacter cloacae and five were due to ent. aerogenes. enterobacter bacteraemia was more often of nosocomial origin than e. coli bacteraemia a ...19921348068
effects of azadirachtin on rhodnius prolixus: immunity and trypanosoma interaction.the effects of azadirachtin, a tetranortriterpenoid from the neem tree azadirachta indica j., on both immunity and trypanosoma cruzi interaction within rhodnius prolixus and other triatomines, were presented. given through a blood meal, azadirachtin affected the immune reactivity as shown by a significant reduction in numbers of hemocytes and consequently nodule formation following challenge with enterobacter cloacae beta 12, reduction in ability to produce antibacterial activities in the hemoly ...19921342719
in vitro antibacterial activities of ticarcillin alone and ticarcillin plus clavulanic acid against beta-lactamase producing and non-producing microorganisms.a total of 818 clinical bacterial isolates were tested for the production of beta-lactamase by rapid chromogenic cephalosporin method and for the susceptibility to ticarcillin alone and in combination with clavulanic acid (2 micrograms/ml) by agar dilution method. these included 83 strains of methicillin-sensitive staphylococcus aureus (mssa), 31 of methicillin-resistant s. aureus (mrsa), 49 of neisseria gonorrhoeae, 58 of haemophilus influenzae, 112 of escherichia coli, 118 of klebsiella pneumo ...19921342000
diffusion of beta-lactam antibiotics into proteoliposomes reconstituted with outer membranes of isogenic imipenem-susceptible and -resistant strains of enterobacter cloacae.the influence of outer membrane (om) permeability on carbapenem susceptibility was studied in strains of enterobacter cloacae, a species in which carbapenem resistance depends upon the conjunction of overproduction of the chromosomal cephalosporinase and reduction of om permeability. relative trans-om diffusion rates were measured using the liposome swelling assay. proteoliposomes were reconstituted with om from the members of an isogenic set of e. cloacae strains, selected in vivo or in vitro, ...19921336033
epidemiological survey of genes encoding aminoglycoside phosphotransferases aph (3') i and aph (3') ii using dna probes.the epidemiological survey of aph (3') i and aph (3') ii genes, at a time when the specific antibiotic pressure was very low, was carried out by dna-dna hybridization. the sample included 334 aminoglycoside resistant gram-negative bacteria collected from patients of a general hospital. of these, 251 hybridized with the aph (3') i-probe and 19 with the aph (3') ii-probe but only 190 strains showed high resistance levels (cim greater than 64 micrograms/ml) for kanamycin, neomycin and paromomycin. ...19921328557
comparative in vitro activity of sparfloxacin (at 4140, rp 64206--spfx) against 275 multiresistant clinical isolates.the in-vitro activity of sparfloxacin was compared with that of pefloxacin, ofloxacin, ciprofloxacin, imipenem, ceftazidime, gentamicin and amikacin against 275 multiresistant nosocomial clinical isolates. they consisted of pseudomonas aeruginosa (37), enterobacter cloacae (42), acinetobacter anitratus (60), klebsiella pneumoniae (37) and staphylococcus sp (99). minimum inhibitory concentrations (mic90) and geometric mean mics for sparfloxacin were as follows (mg/l): p. aeruginosa 128-23.7, e. c ...19921328554
diversity of aminoglycoside resistance in enterobacter cloacae in greece.ninety enterobacter cloacae strains isolated from 12 greek hospitals were examined in terms of epidemiological types and resistance mechanisms. using o serotyping 69% of the strains were assigned to a specific serotype and overall 16 different serotypes were identified. the combination of serotyping, phagetyping and biotyping efficiently discriminated most of the strains, indicating that single epidemic strains were not prevalent, although serotypes 3, 7, and group ii predominated. eight represe ...19921327784
plasmid profiles of acinetobacter and enterobacter species of hospital origin: restriction endonuclease analysis of plasmid dna and transformation of escherichia coli by r plasmids.a total of 37 multi-resistant strains (20 acinetobacter calcoaceticus and 17 enterobacter cloacae) were isolated from patients of the intensive care units. all the isolates were examined for resistance to a battery of antimicrobial agents by the disk diffusion method. plasmid profiles and restriction endonuclease analysis of plasmid dna by ecor1 revealed the spread of one a. calcoaceticus and two e. cloacae endemic strains. transformation experiments on escherichia coli competent cells by three ...19921321240
detergent-shock response in enteric bacteria.our work on bacterial detergent resistance started with the realization that bacteria growing in a sink full of soap must be resistant to the detergents in that soap. we chose sodium dodecyl sulphate (sds) as a model detergent and decided to see how much sds the bacterium growing in the sink could tolerate. the research program thus initiated has shown that bacteria such as enterobacter cloacae can grow in up to 25% sds and that sds-shock proteins constitute c. 8% of the proteins synthesized by ...19921316532
detection of ciprofloxacin resistance in gram-negative bacteria due to alterations in gyra.two plasmids containing the cloned escherichia coli wild-type gyra gene were used to transform ciprofloxacin-resistant gram-negative clinical isolates to screen for dna gyrase a-mediated quinolone resistance. the results show that the technique is simple and applicable to a wide range of gram-negative species including e. coli, enterobacter cloacae, klebsiella aerogenes, morganella morganii, proteus mirabilis, pseudomonas aeruginosa, campylobacter jejuni and neisseria gonorrhoeae. the use of an ...19921310671
exposure effects of netilmicin on enterobacter cloacae clinical isolates.the effects of netilmicin exposure on two aminoglycoside (amg)-resistant and two amg-susceptible enterobacter cloacae isolates were studied. it was found that 1 h incubation of bacterial suspensions with netilmicin at 16 mg/l reduces the amounts of lipopolysaccharides present on the bacterial surface. also, netilmicin pretreatment increased both the susceptibility to normal human serum and the adhesion to human epithelial cells of all four e. cloacae strains examined.19921288965
beta-lactamase expression and cross-resistance to beta-lactam antibiotics in a nosocomial population of enterobacter.this study sought to quantitatively describe the spectrum of constitutive and inducible beta-lactamase activity present in a tertiary care center's population of enterobacter species. beta-lactamase activity in the absence and presence of 2 recognized beta-lactamase-inducing antibiotics, cefoxitin and imipenem, was measured. the enterobacter cloacae (n = 35) population was strikingly bimodal, expressing 'all-or-none' cross-resistance to beta-lactams (except imipenem) corresponding to the baselin ...19921286572
endocarditis due to accidental penetrating foreign bodies.a 15 year old boy had an eight month history of recurrent fever, malaise and poor appetite. chest roentgenogram revealed a foreign object overlying the right ventricle. multiple blood cultures grew enterobacter cloacae. the patients condition improved and blood cultures became negative following gentamicin and carbenicillin therapy. e. cloacae was isolated from the foreign body (a finishing nail) at surgery. antimicrobial therapy was continued for a total of 30 days, and the patient made an unev ...19761274992
nationwide epidemic of septicemia caused by contaminated intravenous products. i. epidemiologic and clinical features.between mid-1970 and april 1, 1971, enterobacter cloacae or e. agglomerans septicemia developed in 378 patients in 25 american hospitals while they were receiving intravenous products manufactured by one company. each of the hospitals noted a marked increase in the incidence of such septicemia during this period. enterobacter agglomerans (formerly designated erwinia, herbicola-lathyri group) was better known as a plant pathogen and had been a human blood pathogen only rarely in the past. septice ...19761274981
relative morphological effects induced by cefoxitin and other beta-lactam antibiotics in vitro.cefoxitin, a new semisynthetic cephamycin antibiotic, induced filament formation at subinhibitory concentrations with a beta-lactamaseless strain of enterobacter cloacae (hsc 18410 m66). the extent of filament induction by cefoxitin was similar to that seen with cephalothin, cefazolin, and benzylpenicillin. filament induction by cefoxitin was markedly less than that seen with cephalexin, carbenicillin, ticarcillin, cephradine, and cephapirin. antibiotics which failed to induce filaments at any l ...19761267431
characterization and replication control of plasmids from enterobacter cloacae df 13.it has been shown previously that enterobacter clocacae df13 harbours at least five different size classes of plasmids. a 45 x 10(6)-mr self-transmissible r factor determining resistance against tetracyclin, sulfanilamide, streptomycin and chloramphenicol, a 6.0 x 10(6)-mr bacteriocinogenic factor without sex factors activity and cryptic plasmids in the size classes of mr 1.3 x10(6), 2.8 x 10(6) and 8.0 x 10(6) respectively. the present work deals with the determination of the homogeneity and mo ...19761261536
dna relatedness among species of enterobacter and serratia.species of enterobacter and serratia were examined for deoxyribonucleic acid relatedness to klebsielleae, to atypical erwiniae, and to other members of enterobacteriaceae. deoxyribonucleic acid hybridization and then hydroxyapatite chromatography was the technique used to assess relatedness. strains of enterobacter cloacae formed two separate hybridization groups that correlate with the presence or absence of yellow pigment. pigmented e. cloacae were 75-100% related, but they were only 40-50% re ...19761260520
nationwide epidemic of septicemia caused by contaminated intravenous products: mechanisms of intrinsic contamination.between 1 july 1970 and april 1971, in many hospitals in this country, there were outbreaks of nosocomial septicemia caused by enterobacter cloacae of e. agglomerans (formerly erwinia, herbicola-lathyri). all of these hospitals used infusion products manufactured by one company, abbott laboratories, and all affected patients had onset of septicemia while receiving the company's infusion products. septicemia was epidemiologically and microbiologically traced to intrinsic contamination of the comp ...19751206107
induction of citrate lyase in enterobacter cloacae grown under aerated conditions and its effect on citrate metabolism.growth of enterobacter cloacae on k+ citrate under aerated conditions (no detectable oxygen tension in the medium even though it was aerated) was slower (mean generation time, 130 min) than under aerobic conditions (mean generation time, 72 min), but with a faster utilization of citrate, resulting in a molar growth yield of 10.6 g (dry weight) of cells per mol of citrate utilized versus 40 g (dry weight) of cells per mol of citrate utilized for aerobic growth. the rapid utilization of citrate un ...19751194231
[enterobacter cloacae bacteremia in dialysis patients]. 19751162135
effects of chemotherapeutics on bacterial ecology in the water of ponds and the intestinal tracts of cultured fish, ayu (plecoglossus altivelis).drug-resistant gram-negative bacilli conferred with r factors were isolated with high frequencies from the intestinal tracts of ayu (plecoglossus altivelis) cultured in ponds, in which chemotherapeutics had often been used, and with relatively low frequencies from ayu which received no administration of chemotherapeutics. drug-resistant bacteria were also isolated at low frequencies from the intestinal tracts of wild ayu in rivers, as well as from the water of ayu-culturing ponds and some of the ...19751160204
electron microscopic appearance of silver sulfadiazine-treated enterobacter cloacae.upon exposure of enterobacter cloacae silver sulfadiazine, a number of ultrastructural changes involving the cell envelope take place. foremost among these is a modification of the cell wall from an undulating structure to one which is smooth and has become enlarged. strains of e. cloacae resistant to silver sulfadiazine do not exhibit these changes.19751157574
massive empyema due to citrobacter diversus.citrobacter diversus is a gram-negative rod member of the enterobacteriacease family. a patient is described from whom this organism was isolated twice in pure culture from empyema fluid. our isolates of citrobacter diversus were resistant to ampicillin and carbenicillin and sensitive to cephalothin. citrobacter diversus should be distinguished from citrobacter freundii, enterobacter cloacae and klebsiella pneumoniae. this organism joins streptococcus pyogenes, bacteroids species, anaerobic stre ...19751149508
pyruvate-uridine diphospho-n-acetylglucosamine transferase. purification to homogeneity and feedback inhibition.phosphoenolpyruvate:uridine-5'-diphospho-n-acetyl-2-amino-2-deoxyglucose-3-enolpyruvyltranferase catalyzes the transfer of enolpyruvate from phosphoenolpyruvate to uridine diphospho-n-acetylglucosamine with the liberation of inorganic orthophosphate. it was purified to homogeneity from enterobacter cloacae with the use of udp-n-acetylmuramyl-l-ala-d-glu-meso-dap, a feedback inihibitor, as a ligand covalenty bound to sepharose 4b. the evidence suggests that the enzyme is a single polypeptide with ...19751123336
tryptophan biosynthetic pathway in the enterobacteriaceae: some physical properties of the enzymes.several physical properties of the first four enzymatic activities of the tryptophan pathway were examined using gel filtration and ion exchange chromatography. five different patterns were noted. differences in the anthranilate synthetase (as) and phosphoribosylanthranilate transferase (prt) defined these patterns. in all the organisms studied phosphoribosylanthranilate isomerase and indoleglycerol phosphate synthetase co-eluted from both diethylaminoethyl-cellulose and g-200 and thus probably ...19751116988
antibodies against enteric bacteria in brown bullhead catfish (ictalurus nebulosus, lesueur) inhabiting contaminated waters.brown bullhead catfish were collected from sewage- and acid mine waste-polluted waters in an attempt to detect antibodies against human enteric bacteria in their sera and to investigate the association of antibody response with environmental conditions. agglutination antigens prepared from isolates obtained from water collected at the same locations as the fish habitats were used to demonstrate such antibodies. the results showed large percentages of reactive sera for common isolates such as esc ...19751099987
membrane filter procedure for enumerating the component genera of the coliform group in seawater.a facile, quantitative, membrane filter procedure (mc) for defining the distribution of coliform populations in seawater according to the component genera was developed. the procedure, which utilizes a series of in situ substrate tests to obviate the picking of colonies for identification, also provides an estimate of the total coliform density. when pure cultures of escherichia coli, klebsiella pneumoniae, and enterobacter cloacae were suspended in seawater and held at 4 c for 24 h, between 56 ...19751098575
r factor in enterobacter cloacae resistant to silver sulfadiazine.a strain of enterobacter cloacae resistant to silver sulfadiazine was recovered from a unit in which this antimicrobial agent was in use. this strain was found to harbor an r factor responsible for resistance to carbenicillin, kanamycin and ampicillin. these antibiotic resistances were transferable to escherichia coli by mating. resistance to silver sulfadiazine was not, however, transmissible.19751097208
mode of action of the cloacin df13-immunity protein.1. cells of enterobacter cloacae harbouring the bacteriocinogenic factor clo df13 produce an immunity protein which inbhbits the in vitro activity of cloacin df13. the amino acid composition of purified immunity protein was determined. 2. experiments about the protection of ribosomes against cloacin df13 in the presence of the immunity protein show that one molecule of immunity protein neutralized the activity of one molecule cloacin. 3. direct and specific interaction of cloacin df13 with the i ...19751092372
stereoselective reduction of cobalt(iii) complexes by bacteria.a strain of enterobacter cloacae, isolated from soil, reduces racemic abdtriglycinatocobalt(iii), triglycinatocobalt(iii), stereoselectively under anaerobic conditions; washed suspensions oc cells, grown anaerobically in the absence of triglycinatocobalt(iii) initially reduce the l-enantiomer stereospecifically under argon yielding a labile co(ii) chelate. an approximately 5-fold increase in the rate of reduction of the complex occurs using cells disrupted by 2-phenylethanol; decomposition now p ...19751092371
control of salmonellosis pacifarin biosynthesis by iron.enterobacter cloacae strain ss4-56 produced salmonellosis pacifarins when grown in an iron-depleted synthetic medium to which 3.16 x 10-7 g-atom of iron had been added per liter, but did not do so when grown in iron-depleted medium supplemented with 3.16 x 10-4 g-atom of iron per liter. the addition of 3.16 x 10-4 g-atom of ferric iron per liter to a pacifarin-containing culture supernatant fluid had no significant effect upon the ability of the active pacifarins present, when administered per o ...19751090527
amikacin in the treatment of gram-negative bronchopulmonary infections.six patients with acute gram-negative bronchopulmonary infection were treated with amikacin (15 mg/kg per day) administered intramuscularly in two equal doses at 12-hr intervals for 10-13 days. two patients had underlying nonspecific pulmonary disease, two had advanced bronchocarcinoma, and two had extensive bronchiectasis (due to chronic aspergillosis in one patient). the pathogens were pseudomonas aeruginosa in three patients, and haemophilus influenzae, klebsiella ozaenae, and enterobacter cl ...19761086876
complement consumption in fresh blood sera by double culture of paramecium caudatum and aerobacter cloacae compared with survival and stage of disease in some malignant tumors in man.using the complement consumption test by double culture of paramecium caudatum and aerobacter cloacae (dpa) as antigen we examined blood sera and compared survival rates and stage of disease in 124 patients with carcinoma of cervix uteri, corpus uteri, ovaries and breast. the evidence suggests that bacteria aerobacter cloacae constitute the proper antigenically effective substances of dpa. the intensity of reaction and therapy the relative titres of antibodies detected by the test were expressed ...19761084486
[taxonomic importance of fermentation balances of enterobacteriaceae (author's transl)].fermentation balances have been studied on 66 strains from 9 taxa of enterobacteriaceae. in addition, 6 strains of aeromonas have been investigated. among these fermentation balances, 7 types could be distinguished. it was shown, that the taxa of enterobacteriaceae differed with respect to the balance types involved. this is true especially for vp-positive taxa. for example, hafnia, the klebsiella-enterobacter-serratia group, enterobacter cloacae and erwinia are to some extend characterized by d ...1976998036
substrate inhibition of beta-lactamases, a method for predicting enzymatic stability of cephalosporins.selected cephalosporins, including cefamandole, cephaloridine, cephaloglycin, and cefoxitin, were examined for their ability to inhibit the enzymatic activity of and act as substrates for beta-lactamases produced by enterobacter cloacae and staphylococcus aureus. enzyme inhibition was determined by michaelis-menten kinetic measurements and by a spot plate assay using a chromogenic substrate (glaxo compound 87/312). these two methods provide comparable estimates of kinetic parameters. inhibition ...1976984790
the fate of bacteria in frozen red cells.units of blood were intentionally contaiminated with suspensions of either aerobacter aerogenes, escherichia coli, bacillus subtilis, enterobacter cloacae, pseudomonas aeruginosa, sarcina lutea, seratia marcesens, staphylococcus epidermidis, streptococcus faecalis, paracolabacterum aerogenoides (enterobacter hafriae), mima polymorpha or acinetobacter- calcoaceticus. when inoculation was made prior to glycerolization, the subsequent glycerolization, freezing, thawing, and deglycerolization result ...1976936269
production, purification, and characterization of excitability-inducing molecule.excitability-inducing molecule (eim) is a high molecular weight polymeric protein. it is a channel-forming ionophore which can induce action potential in lipid bilayers and lyse red blood cells. it is also a potent mitogen for mouse b lymphocytes. eim is produced and secreted into a chemically defined medium during the early growth period by enterobacter cloacae atcc 961. it is now 6000-fold purified as compared to the original egg white eim. production of the active material requires calcium io ...1977925029
host-dependent, thermosensitive replication of an r plasmid, pjy5, isolated from enterobacter cloacae.the thermosensitive replication of an r plasmid, pjy5, isolated from enterobacter cloacae, was studied. pjy5 consisted of 61 million daltons of covalently closed circular (ccc) deoxyribonucleic acid (dna) with a buoyant density of 1.714 g/cm3 (55 mol % guanine plus cytosine). in escherichia coli, this plasmid replicated stringently at 32 degrees c, but ceased its ccc dna replication after a short incubation at 42 degrees c, resulting in production of r- segregants. the thermosensitive replicatio ...1977924975
in vitro activities of five oral cephalosporins against aerobic pathogenic bacteria.cefaclor (lilly 99638) and cefatrizine (bl-s640, sk&f 70771) are orally absorbed, broad-spectrum semisynthetic cephalosporins. they were compared in vitro with cephalexin, cephaloglycin, and cepharadine against a variety of aerobic pathogenic bacteria by an agar dilution procedure. cefaclor and cefatrizine were found to be similar or superior to cephalexin, cephaloglycin, and cephradine in terms of activity against gram-positive cocci other than enterococci. only cefatrizine demonstrated any pot ...1977921258
[role of several conditionally-pathogenic enterobacteria in group acute intestinal diseases and food poisonings of unestablished etiology].results of studying group acute intestinal diseases of obscure (from the point of view of isolation of commonly known pathogenic bacteria of enteric family) etiology coursing by the type of food poisoning, in which conditioned pathogenic enterobacteria (enterobacter cloacae, klebsiella) demonstrated that the occurrence of these diseases was connected with ingestion of the same food product infected with the mentioned bacteria due to disturbance of the sanitary-epidemic regimen and rules of food ...1977919924
enzymatic and immunological characterization of a new cephalosporinase from enterobacter aerogenes.a hospital strain of enterobacter aerogenes (mulb 250) isolated from a urinary tract infection was found to be cephalosporin and ampicillin resistant and carbenicillin susceptible. the beta-lactamase produced by this strain was extracted and purified by means of affinity chromatography, using a cephalosporin c-bound sepharose 4b column. the purified enzyme was tested for hydrolysis of penicillin and various cephalosporins. the k(m) value is 11.8 mum for benzyl penicillin and 130 mum for cephalos ...1977900918
infectious arthritis resulting from enterobacter cloacae and enterobacter hafniae. 1977891089
multiple joint infections with enterobacter cloacae.an infant with multiple joint infections from enterobacter cloacae seems not to have been reported previously. the infant survived, incurred a pathologic dislocation of the left hip, but at 18 months of age was well with all other joints functioning normally.1977880754
clavulanic acid: a beta-lactamase-inhiting beta-lactam from streptomyces clavuligerus.a novel beta-lactamase inhibitor has been isolated from streptomyces clavuligerus atcc 27064 and given the name clavulanic acid. conditions for the cultivation of the organism and detection and isolation of clavulanic acid are described. this compound resembles the nucleus of a penicillin but differs in having no acylamino side chain, having oxygen instead of sulfur, and containing a beta-hydroxyethylidine substituent in the oxazolidine ring. clavulanic acid is a potent inhibitor of many beta-la ...1977879738
intracellular crystalline deposits by bacteria grown in urine from a stone former.several species of bacteria were found to form an intracellular crystalline material when grown in urine obtained from a subject with a history of infrequent renal calculi formation. the following species: proteus mirabilis, proteus rettgeri, providencia stuartii, enterobacter aerogenes, enterobacter cloacae, escherichia coli, and candida albicans formed crystals of hydroxyapatite. klebsiella pneumoniae, pseudomonas aeruginosa, and proteus vulgaris produced crystals of calcite -ii. several of th ...1977844994
antimicrobial susceptibility patterns (antibiograms) as an aid in differentiating citrobacter species.the hydrogen sulfide-negative citrobacter group represents a taxonomic problem. various investigators have proposed such designations as padlewskia, levinea, atypical enterobacter cloacae, h2s-negative variants of citrobacter, citrobacter koseri and citrobacter diversus. this problem has been investigated with emphasis on antibiograms as a means of discrimination. clinical isolates fitting the designation citrobacter were studied and, using the criteria of ewing and davis, separated into two gro ...1977836626
susceptibility of clinically important gram-negative bacilli to 15 antibiotics. comparison of patterns in poland and federal republic of germany.three hundred and sixty strains of gram-negative bacilli (escherichia coli, salmonella enteritidis, enterobacter cloacae, providencia, indole-positive and indole-negative proteus, klebsiella pneumoniae, pseudomonas aeruginosa and serratia marcescens), were isolated by random in poland and germany, and tested by agar dilution method for susceptibility to 15 antimicrobial agents, including six cephalosporin derivatives, colistin, gentamycin and tobramycin, three newer tetracyclines, ampicillin and ...1976824896
interference by neisseria gonorrhoeae growth by other bacterial species.growth of neisseria gonorrhoeae from clinical specimens has been enhanced by the use of selective media that inhibit the simultaneous growth of other microorganisms. one explanation for this enhancement could be that certain other bacteria inhibit gonococcal growth. this hypothesis was examined by testing 167 bacterial isolates for in vitro gonococcal inhibition; 34.1% of the isolates failed to inhibit the gonococcus, but 12.0% produced weak inhibition and 53.9% strongly inhibited n. gonorrhoeae ...1976823175
insect immunity. 11. simultaneous induction of antibacterial activity and selection synthesis of some hemolymph proteins in diapausing pupae of hyalophora cecropia and samia cynthia.we have previously shown that pupae of the giant silkmoth samia cynthia have a humoral antibacterial activity, which was induced by viable, nonpathogenic gram-negative bacteria (h.g. boman et al., 1974). we show here that this activity was formed simultaneously with a selective incorporation of amino acids into eight polypeptide chains characterized by their electrophoretic behavior. if actinomycin d or cycloheximide were given at an early time, no antibacterial activity was found. if the inhibi ...1975812827
laboratory studies with cefatrizine (sk + f 60771), a new broad-spectrum orally-active cephalosporin.cefatrizine (sk&f 60771), a new orally-active semisynthetic cephalosporin antibiotic with broad-spectrum antibacterial activity, was compared with cephalexin and cefazolin for in vitro and in vivo antibacterial activity and pharmacokinetic behavior in laboratory animals. the average mic values obtained with cefatrizine against gram-positive and gram-negative bacteria were superior to those obtained with cephalexin and somewhat poorer than those of cefazolin. in addition, a large percentage of th ...1975808525
disinfection procedures of fiberscopes in endoscopy departments.disinfection of fibre endoscopes was examined in 5 endoscopy departments during endoscopy sessions. on the endoscopes, especially in the instrument channels, pseudomonas aeruginosa and proteus species could be regularly found, in some cases also enterobacter cloacae and candida albicans. with the results of disinfection experiments, proposals are made to reduce the infection risk due to insufficiently disinfected instruments in endoscopy departments.1976799960
transferable gentamicin resistance in enterobacter cloacae. 1976783111
reevaluation of kanamycin dosage in infants and children.it was recently reported that the dosage of kanamycin formerly recommended for neonates of 7.5 mg/kg every 12 h did not produce therapeutic serum concentrations of 15 to 25 mug/ml, but a larger dosage of 10 mg/kg every 12 h was required. reevaluation of the pharmacokinetics of kanamycin in infants and children was therefore undertaken. sixteen time-concentration curves after a dose of 5 mg/kg were obtained from patients 2 months to 12 years of age; the mean peak serum kanamycin concentration was ...1976779634
instability and linkage of silver resistance, lactose fermentation, and colony structure in enterobacter cloacae from burn wounds.a strain of enterobacter cloacae from a burn wound has displayed unstable resistance to silver nitrate and a linkage of this characteristic to that for rapid lactose fermentation and mucoidcolony structure. attempts to demonstrate transferability of these unstable determinants gave negative results.1976777048
[relation of acetoin and valine in enterobacteriaceae cultures on media with glucose and glycerin].the ratio between acetoin and valine produced by the cultures of the family enterobacteriaceae changes upon the addition of glucose and glycerol or their mixture to a defined medium. the experiments were carried out with 12 strains of aerobacter aerogenes and 2 strains of aerobacter cloacae and serratia marcescens. the highest yield of acetoin was found on the medium with glucose (12.5--13.0 g/litre); the maximum accumulation of valine was registered on the medium with glucose and glycerol (1.8- ...1975775256
failure of disposable domes to prevent septicemia acquired from contaminated pressure transducers.between january and june 1976, eight cases of primary bacteremia due to enterobacter cloacae occurred in patients after open-heart surgery in a hospital in connecticut. epidemiologic studies implicated radial arterial catheters as the route of acquistion of e cloacae, and bacteriologic studies confirmed arterial pressure transducers as the sources of bacteria. prospective studies indicated that the disposable domes from the pressure transducers did not prevent the spread of bacteria from contami ...1978738087
[numerical taxonomic studies on enterobacteria of surface-water (author's transl)].435 enterobacterial strains of surface-water were tested in 35 physiological characters and classified numerically by a single-linkage procedure. the relations of similarity are shown in a "vernetzungsdiagramm" (connected graph, fig. 1). tab. 1 specifies the characters of the taxa, which were formed graphically. the following facts are the result of this taxonomic classification: 1. 32% of the escherichia group are kcn-positive. in addition it was found that 60% of the e. coli isolated from the ...1978735569
[growth of some pathogenic microorganisms on chemically defined media which were proposed for sensitivity testing (author's transl)].the growth of 16 bacterial cultures, which belong to the frequently encountered pathogens, in three different chemically defined fluid media was investigated through turbidity measurements in comparison with mueller-hinton-broth. e. coli, salm. dublin, salm. typhi-murium, enterobacter cloacae, proteus mirabilis, pseud. aeruginosa and bac. cereus grew in two of the three tested defined media with approximately the same intensity as in the mueller-hinton broth. staph. aureus grew well only in one ...1978730218
laboratory-scale production of acetoin plus diacetyl by enterobacter cloacae atcc 27613.conditions for the laboratory-scale production of acetoin plus diacetyl by enterobacter cloacae atcc 27613 were studied. thirty-five g acetoin plus diacetyl/50 g sucrose were obtained when fermentation was carried out in 2.5 liter medium containing 12.5 g peptone and 12.5 g yeast extract, at ph 7.0, in a 5 liter conical flask on a shaker (240 rpm) at 28-30 degrees c for 48 hr. recovery of pure diacetyl was 85% of the total acetoin plus diacetyl.1978728550
standardization of a rapid microbiologic assay for aminoglycosides using enterobacter cloacae.the standardization of a rapid serum aminoglycoside assay using enterobacter cloacae is described. this includes the sensitivity testing of the organism and its performance on various media, with mueller-hinton agar being the medium of choice. the precision and reproducibility of the assay, as measured by the within-run and between-run coefficients of variation, were 5.0 and 5.9, respectively. a significant positive correlation was obtained between the microbiologic assay for gentamicin and a 12 ...1978717287
gas liquid chromatography for detection of bacteriuria: examination for volatile acidic and neutral compounds.direct injection of clinically infected urines on porous-polymer columns was investigated to determine which microbial metabolites were consistently detectable, and whether their presence could be used as a reliable index of infection. chromosorb 101 was found to be the most suitable porous polymer for the detection of microbial metabolites; greater sensitivity of detection was achieved by partial purification of the urine before injection. acetic acid was the only compound found consistently an ...1978711917
neonatal meningitis due to enterobacter cloacae.enterobacter species, in recent years, have been divided into e aerogenes, e hafniae, e liquefaciens, and e cloacae. early reviews of neonatal meningitis include some cases due to klebsiella-aerobacter,(1) and recent reviews(2,3) include enterobacter which did not divide them into species of enterobacter. reported here is a case of neonatal meningitis due to a gram-negative organism, enterobacter cloacae.1978702571
resistant enterobacter cloacae in a burn center: the ineffectiveness of silver sulfadiazine.enterobacter cloacae sepsis was found in 15 burn center patients in 1976, of whom 13 died. nine of the deaths occurred in the first 60 days. the burn center isolates were resistant to silver sulfadiazine (agsd) in agar cup-plate tests and confirmed by tube dilution tests. hospital, non-burn isolates of e. cloacae were sensitive to agsd. all e. cloacae isolates were sensitive to mafenide acetate (ma) in the agar cup-plate tests, but this was not confirmed by the tube dilution tests. the agar cup- ...1978660684
r factors from enterobacter group.twenty-six r factors transmissible to e. coli k12 were derived from 79 strains of enterobacter species isolated in various clinical specimens (urine, sputum, blood, pus); 71 of 79 strains were enterobacter cloacae. fifteen r factors were fi+: 14 belonged to group fii, 1 to group fi. eleven r factors were fi-: 5 belonged to group m, of the others 6 (all controlling the cloramphenicol-resistance) 1 was recognized of group k, 1 was incompatible with standard plasmids of both groups s and w, and 4 b ...1978656192
heterotrophic bacteria present in hindguts of wood-eating termites [reticulitermes flavipes (kollar)].strict anaerobic culture techniques were used to quantitate heterotrophic bacteria present in hindguts of reticulitermes flavipes. the grand mean number of viable cells per hindgut was 0.4 x 10(5) (first-instar larvae), 1.3 x 10(5) (third-instar larvae), 3.5 x 10(5) (workers), and 1.5 x 10(5) (soldiers). of a total of 344 isolates, 66.3% were streptococci that were always obtained regardless of the origin of termites, their developmental stage or caste, or their length of captivity. most of the ...1978655709
bacterial colonization of jejunal mucosa in giardiasis.nine of 14 cases of giardiasis and severe malabsorption were found to have numerous bacteria adjacent to the mucosa and within luminal fluid samples from the upper jejunum. three species of enterobacteria (klebsiella pneumoniae, enterobacter cloacae and e. hafniae) were cultured from eight patients and from only one were bacteroides isolated. enterobacteria were not cultured from seven of eight patients who had giardiasis but only mild malabsorption (of xylose only) nor from seven patients witho ...1978635972
nitrogen fixation (acetylene reduction) associated with roots of winter wheat and sorghum in nebraska.root segments and root-soil cores (6.5-cm diameter) from fields and nurseries of winter wheat and sorghum were tested for n2 fixation by using the acetylene reduction assay. wheat samples (approximately 1,200) from 109 sites generally had low or no activity (0 to 3.1 nmol of c2h4 produced per h per g [dry weight] of root segments), even after 24 h of incubation. however, a commercial field of scout 66, located in western nebraska, exhibited appreciable activity (290 nmol of c2h4 produced per h p ...1978623458
clinical study of the use of the new aminoglycoside tobramycin for therapy of infections due to gram-negative bacteria.tobramycin, a new aminoglycoside antimicrobial, was evaluated as therapy for infections due to gram-negative bacilli in 15 seriously ill patients with underlying diseases. three of 10 patients with gram-negative bacteraemia also had a urinary tract infection. two patients had a respiratory tract infection, one had cellulitis, one had a urinary tract infection, and another had septic phlebitis. twelve patients were cured of their infections. eosinophilia was observed in one patient, and another d ...1977600202
the growth and respiration of bacterial colonies.young colonies of two swarming organisms, bacillus subtilis and proteus vulgaris, grew about as quickly on solid media as in liquid culture whilst four non-swarming organisms, bacillus cereus, enterobacter cloacae, escherichia coli and staphylococcus albus, all grew slower on solid than in liquid media. oxygen uptake by young colonies of b. subtilis, followed manometrically, increased exponentially at about the same rate as unrestricted aerobic growth. all other colonies demonstrated acceleratin ...1977591923
enterobacteria of emerging pathogenic significance from clinical cases in man and animals and detection of toads and wall lizards as their reservoirs.a total of 416 samples comprising faecal samples from diarrhoeic cases of man, calves, sheep and goats, and urine samples from patients with urinary tract infections, were examined for the presence of enterobacteria of emerging pathogenic significance. citrobacter freundii from 20, c. intermedius biotype-a from four, serratia marcescens (serotype 05:h13, bactericin type 16) from one and erwinia herbicola from two human stool samples were isolated. only two urine samples yielded c. freundii. citr ...1978582096
interaction of acetoin precursors with pyruvate metabolism of torulopsis colliculosa & enterobacter cloacae. 1978570546
[synthesis and biological activity of 7-(2-nitroimidazoles and 7-(2-aminoimidazole) methyleneamino cephalosporin and their derivatives].the synthesis of 7-[[(1-methyl-2-nitro-1h-imidazol-5-yl)methylene]amino] and 7-[[(2-amino-1-methyl-1h-imidazol-5-yl)methyle-ne]amino]cephalosporanic acids and of some derivatives is described. their physico-chemical characteristics are reported. the compounds show no appreciable antibacterial activity in vitro. they show no synergy with cephaloridine against enterobacter cloacae 214 (producer of class i beta-lactamase) and therefore have no anti-beta-lactamase activity.1979553829
biochemical differentiation in large colonies of enterobacter cloacae.large colonies of enterobacter cloacae which were about 700 micrometer thick were frozen in liquid nitrogen and sectioned horizontally. the sections were disrupted and several oxidative enzymes were assayed in the crude unfractionated homogenates. in the top 120 micrometer of the colonies the specific activities of the enzymes were high and characteristic of aerobically adapted cells. cells nearer the base of colonies had very low enzyme activities.1979541662
[pharmacokinetics of a new cephalosporin, cefoperazone].cefoperazone is a semi-synthetic cephalosporin for parenteral use with an extended antibacterial spectrum covering pseudomonas aeruginosa, enterobacter cloacae and serratia marcescens. its pharmacokinetic properties were studied in 8 healthy subjects after 2 intravenous infusions of 2 g of the drug at a 12-hour interval. the mean peak serum concentrations were 134 +/- 16 microgram/ml and 143 microgram/ml. cefoperazone was shown to possess a long half-life for a cephalosporin (1.7 hours). in our ...1979538442
silver-resistant enterobacteriaceae from hospital patients.the inclusion of agar medium containing 0.5 mm agno3 in the hospital laboratory replicating system for routine antibiotic-susceptibility determinations resulted in identification of species of enterobacteriaceae (escherichia coli, enterobacter cloacae, klebsiella pneumoniae, proteus mirabilis, and citrobacter freundii) with silver resistance. since the study began in october, 1975, 11 in-hospital patients receiving silver sulfadiazine for burn wound prophylaxis have yielded silver-resistant bact ...1979526888
bacteremia associated with enterobacter sakazakii (yellow, pigmented enterobacter cloacae).a case report of bacteremia due to enterobacter sakazakii, listed previously as yellow-pigmented enterobacter cloacae (r. sakazaki, in r. e. buchanan and n. e. gibbons, ed., bergey's manual of determinative bacteriology, 8th ed., p. 325, 1974), occurred in a 7-day-old, caucasian male who responded successfully to ampicillin therapy. the source of the infection was not known; however, because of the time lapse between birth and the onset of symptoms, the infection was thought to have occurred pos ...1979521484
[rahnella aquatilis, a new member of the enterobacteriaceae (author's transl)].a dna-dna hybridization study was carried out to determine the taxonomic position of a new group of enterobacteria (group h2) previously studied by numerical taxonomy. all the strains of this group revealed relatively high reassociation binding ratios with the centrotype; 82% of the strains of the group showed more than 69% of reassociation with the centrotype. in spite of numerical taxonomy conclusions, there was no genetic relationship with the species enterobacter cloacae (higher reassociatio ...1979484990
[study of a new group of enterobacteriacea (group h1) related to enterobacter cloacae strain].a dna-dna hybridization study was carried out on a new group of enterobacteria (group h1) previously studied by numerical taxonomy work on the genus enterobacter. this group showed a very high genetic homogeneity since the average relative binding ratio of nine analysed strains is equal to 91%. the taxonomic position of this group into the family of enterobacteria is discussed with the species e. cloacae (37 to 61%), k. pneumoniae (44 to 60%), k. oxytoca (57-58%), l. malonatica (syn. citrobacter ...1979476548
enterobacter cloacae septicemia in a burn center: epidemiology and control of an outbreak.an outbreak of infections due to enterobacter cloacae occurred in the burn center at the medical college of virginia (richmond, virginia) in 1976. fifteen patients had bacteremia due to e. cloacae; 10 cases of bacteremia occurred during a six-week period in january and february. the development of bacteremia was significantly related to the extent of third-degree burn and to admission to the burn center in january and february but not to the presence of an intravenous cannula, underlying disease ...1979438531
rapid enumeration of fecal coliforms in water by a colorimetric beta-galactosidase assay.the colorimetric beta-galactosidase assay is based upon the enzymatic hydrolysis of the substrate o-nitrophenyl-beta-d-galactoside (onpg) by fecal coliforms. this technique provides an estimate of the fecal coliform concentration within 8 to 20 h. a 100-ml portion of test sample was passed through a 0.45 micrometer membrane filter. this filter was then incubated at 37 degrees c for 1 h in ec medium followed by the addition of filter-sterilized onpg. the incubation was continued at 44.5 degrees c ...1978414659
effect of aerosol immunization with re 595 salmonella minnesota on lung bactericidal activity against serratia marcescens, enterobacter cloacae, and pseudomonas aeruginosa.intrapulmonary bactericidal activity was measured after mice were given 3 weekly aerosol exposures to acid-hydrolyzed re 595 salmonella minnesota. ten days after their last immunization, mice were challenged with aerolized serratia marcescens, enterobacter cloacae, or pseudomonas aeruginosa. quantitative bacterial counts in ground lung were obtained immediately after exposure and again 4 hours later. enhanced bactericidal activity against serratia marcescens and enterobacter cloacae was seen in ...1977407823
synergy between ticarcillin and tobramycin against pseudomonas aeruginosa and enterobacteriaceae in vitro and in vivo.the antibacterial activities of ticarcillin, carbenicillin, tobramycin, and gentamicin and of combinations of these antibiotics were measured against pseudomonas aeruginosa and other gram-negative bacilli in vitro and in experimental mouse infections. synergistic effects were produced by the penicillin/aminoglycoside combinations in growth inhibition tests and in bactericidal tests against many of the bacteria tested. combinations of ticarcillin + tobramycin were more active in vitro than carben ...1977406837
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