Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| [autonomic dysfunction in acute inflammatory demyelinating polyneuropathy]. | acute inflammatory demyelinating polyneuropathy (aidp) is an autoimmune process that is characterized by progressive weakness, mild sensory changes and autonomic dysfunction. it is a rare disorder, afflicting about 1 person in 100,000. yet, since the decline in the number of polio cases, it represents the most common cause of acute neuromuscular paralysis. aidp is thought to arise as a result of the production of antibody to bacterial campylobacter jejuni, sugar-containing surface antigen(s) tha ... | 2004 | 15688775 |
| multiple modes of motility: a second flagellar system in escherichia coli. | 2005 | 15687183 | |
| incomplete reiter's arthritis in a 23 year old nursing mother. | 2004 | 15687073 | |
| incidence and mechanism of ciprofloxacin resistance in campylobacter spp. isolated from commercial poultry flocks in the united kingdom before, during, and after fluoroquinolone treatment. | five commercial broiler flocks were treated with a fluoroquinolone for a clinically relevant infection. fresh feces from individual chickens and environmental samples were cultured for campylobacters before, during, and weekly posttreatment until slaughter. both campylobacter jejuni and c. coli were isolated during all treatment phases. an increased proportion of quinolone-resistant strains was seen during treatment, and these strains persisted posttreatment. one quinolone-resistant isolate of e ... | 2005 | 15673754 |
| prevalence and subtypes of ciprofloxacin-resistant campylobacter spp. in commercial poultry flocks before, during, and after treatment with fluoroquinolones. | five commercial broiler chicken flocks were treated with either difloxacin or enrofloxacin for a clinically relevant infection, as instructed by a veterinarian. campylobacters were isolated from individual fecal samples and from samples associated with the broiler environment before, during, and after treatment. ciprofloxacin-resistant campylobacter jejuni and/or c. coli strains were detected pretreatment in four flocks, but they constituted a very small proportion of the campylobacters present. ... | 2005 | 15673753 |
| predicted hexameric structure of the agrobacterium virb4 c terminus suggests virb4 acts as a docking site during type iv secretion. | the agrobacterium t-dna transporter belongs to a growing class of evolutionarily conserved transporters, called type iv secretion systems (t4sss). virb4, 789 aa, is the largest t4ss component, providing a rich source of possible structural domains. here, we use a variety of bioinformatics methods to predict that the c-terminal domain of virb4 (including the walker a and b nucleotide-binding motifs) is related by divergent evolution to the cytoplasmic domain of trwb, the coupling protein required ... | 2005 | 15668378 |
| analysis of the fecal microbiota of irritable bowel syndrome patients and healthy controls with real-time pcr. | the gut microbiota may contribute to the onset and maintenance of irritable bowel syndrome (ibs). in this study, the microbiotas of patients suffering from ibs were compared with a control group devoid of gastrointestinal (gi) symptoms. | 2005 | 15667495 |
| electron transport through nitrate and nitrite reductases in campylobacter jejuni. | campylobacter jejuni is a small genome pathogen that is incapable of growing strictly anaerobically due to its dependence on an oxygen-requiring ribonucleotide reductase for dna synthesis. nevertheless, it has a complex branched respiratory chain, which allows the use of several alternative electron acceptors for growth under oxygen-limited conditions. c. jejuni is equipped with both nitrate reductase (nap) and nitrite reductase (nrf) located in the periplasm, each predicted to receive electrons ... | 2005 | 15667303 |
| nitric oxide and nitrosative stress tolerance in bacteria. | nitric oxide is not only an obligatory intermediate in denitrification, but also a signalling and defence molecule of major importance. however, the basis of resistance to no and rns (reactive nitrogen species) is poorly understood in many microbes. the cellular targets of no and rns [e.g. metalloproteins, thiols in proteins, glutathione and hcy (homocysteine)] may themselves serve as signal transducers, sensing no and rns, and resulting in altered gene expression and synthesis of protective enz ... | 2005 | 15667299 |
| use of molecular hydrogen as an energy substrate by human pathogenic bacteria. | molecular hydrogen is produced as a fermentation by-product in the large intestine of animals and its production can be correlated with the digestibility of the carbohydrates consumed. pathogenic helicobacter species (helicobacter pylori and h. hepaticus) have the ability to use h(2) through a respiratory hydrogenase, and it was demonstrated that the gas is present in the tissues colonized by these pathogens (the stomach and the liver respectively of live animals). mutant strains of h. pylori un ... | 2005 | 15667272 |
| survey of parasites and bacterial pathogens from free-living waterfowl in zoological settings. | to determine if free-living waterfowl residing in a zoological setting pose health risks for its animal collections, visitors, and employees, 450 fecal samples were collected and cultured for the presence of campylobacter jejuni, escherichia coli, salmonella spp., and pasteurella multocida. a survey of endoparasites infecting the waterfowl was also conducted. sixty-seven percent, 42%, and 1.7% of the samples tested positive for e. coli, c. jejuni, and salmonella spp., respectively. no p. multoci ... | 2004 | 15666857 |
| the diversity of dolichol-linked precursors to asn-linked glycans likely results from secondary loss of sets of glycosyltransferases. | the vast majority of eukaryotes (fungi, plants, animals, slime mold, and euglena) synthesize asn-linked glycans (alg) by means of a lipid-linked precursor dolichol-pp-glcnac2man9glc3. knowledge of this pathway is important because defects in the glycosyltransferases (alg1-alg12 and others not yet identified), which make dolichol-pp-glycans, lead to numerous congenital disorders of glycosylation. here we used bioinformatic and experimental methods to characterize alg glycosyltransferases and doli ... | 2005 | 15665075 |
| major structural differences and novel potential virulence mechanisms from the genomes of multiple campylobacter species. | sequencing and comparative genome analysis of four strains of campylobacter including c. lari rm2100, c. upsaliensis rm3195, and c. coli rm2228 has revealed major structural differences that are associated with the insertion of phage- and plasmid-like genomic islands, as well as major variations in the lipooligosaccharide complex. poly g tracts are longer, are greater in number, and show greater variability in c. upsaliensis than in the other species. many genes involved in host colonization, in ... | 2005 | 15660156 |
| direct observation of the participation of flavin in product formation by thyx-encoded thymidylate synthase. | the synthesis of thymine for dna is catalyzed by the enzyme thymidylate synthase (ts). a family of flavin-dependent tss encoded by the thyx gene has been discovered recently. these newly discovered tss require a reducing substrate in addition to 2'-deoxyuridine monophosphate (dump) and 5,10-methylenetetrahydrofolate (ch2thf), suggesting that the enzyme-bound flavin is a redox intermediary in catalysis. the oxidation of the reduced flavin of the ts from campylobacter jejuni has been observed dire ... | 2005 | 15656610 |
| destabilization of tetranucleotide repeats in haemophilus influenzae mutants lacking rnasehi or the klenow domain of poli. | a feature of haemophilus influenzae genomes is the presence of several loci containing tracts of six or more identical tetranucleotide repeat units. these repeat tracts are unstable and mediate high frequency, reversible alterations in the expression of surface antigens. this process, termed phase variation (pv), enables h.influenzae to rapidly adapt to fluctuations in the host environment. perturbation of lagging strand dna synthesis is known to destabilize simple sequence repeats in yeast and ... | 2005 | 15653640 |
| an annotated checklist of pathogenic microorganisms associated with migratory birds. | the potential for transport and dissemination of certain pathogenic microorganisms by migratory birds is of concern. migratory birds might be involved in dispersal of microorganisms as their biological carriers, mechanical carriers, or as carriers of infected hematophagous ecto-parasites (e.g., ixodid ticks). many species of microorganisms pathogenic to homeothermic vertebrates including humans have been associated with free-living migratory birds. migratory birds of diverse species can play sig ... | 2004 | 15650082 |
| investigation of possible cytokine induction in peripheral blood mononuclear cells by heat-stable serotypes of campylobacter jejuni. | several campylobacter jejuni heat-stable (hs) serotypes have been associated with the autoimmune guillain-barre neurological syndrome (gbs). in order to examine the possible involvement of cytokines in this phenomenon, the levels of three pro-inflammatory cytokines (interleukin (il)-2sra, il-6 and interferon (ifn)-gamma) and one anti-inflammatory cytokine (il-10) were measured in peripheral blood mononuclear cells after induction by different c. jejuni serotypes. no differences were found for il ... | 2005 | 15649306 |
| phenotypic characterisation of flagellin and flagella of urease-positive thermophilic campylobacters. | in this study, flagellin is purified biochemically from eight urease-positive thermophilic camplylobacters (uptc) isolated from river water, sea water and mussels, and purified also from two isolates of campylobacter jejuni and c. coli and fractionated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (sds-page). results showed that no flagellin components were detected in the two japanese uptc isolates (cf89-12 and cf89-14) and the two uptc nctc strains (nctc12893 and nctc12894). fl ... | 2004 | 15649010 |
| phosphonate catabolism by campylobacter spp. | the catabolism of phosphonates (phn) by campylobacter spp. was investigated employing nuclear magnetic resonance spectroscopy and cell culture techniques. the bacteria were capable of cleaving the phn bonds of different compounds, including alpha-aminomethylphosphonate, phosphonoacetate and phenylphosphonate (phephn). the kinetic parameters of these activities were determined in vivo in intact cells and in situ in whole-cell lysates. cleavage of phn-bearing compounds was associated with the cell ... | 2005 | 15647906 |
| seroprevalence of helicobacter pylori infection in urban and rural vietnam. | helicobacter pylori-associated diseases, such as peptic ulcer and gastric cancer, are common in vietnam, but the prevalence of the infection is largely unknown. a validated enzyme-linked immunosorbent assay was used for seroepidemiology with 971 samples from the general population, ages 0 to 88 years, with 546 samples from an urban population (hanoi), and with 425 samples from a poor, rural province (hatay). the overall seroprevalence of the infection was 746 per 1,000, with a prevalence of 788 ... | 2005 | 15642989 |
| arrayome: a program for estimating the sizes of microarray-visualized bacterial genomes. | arrayome is a new program that calculates the size of genomes represented by microarray-based probes and facilitates recognition of key bacterial strains carrying large numbers of novel genes. protein-coding sequences (cds) that are contiguous on annotated reference templates and classified as 'present' in the test strain by hybridization to microarrays are merged into ics (ics). these ics are then extended to account for flanking intergenic sequences. finally, the lengths of all extended ics ar ... | 2005 | 15640440 |
| prevalence and antimicrobial resistance of thermophilic campylobacter spp. from cattle farms in washington state. | the prevalence of thermophilic campylobacter spp. was investigated in cattle on washington state farms. a total of 350 thermophilic campylobacter isolates were isolated from 686 cattle sampled on 15 farms (eight dairies, two calf rearer farms, two feedlots, and three beef cow-calf ranches). isolate species were identified with a combination of phenotypic tests, hipo colony blot hybridization, and multiplex lpxa pcr. breakpoint resistance to four antimicrobials (ciprofloxacin, nalidixic acid, ery ... | 2005 | 15640184 |
| temperature-driven campylobacter seasonality in england and wales. | campylobacter incidence in england and wales between 1990 and 1999 was examined in conjunction with weather conditions. over the 10-year interval, the average annual rate was determined to be 78.4 +/- 15.0 cases per 100,000, with an upward trend. rates were higher in males than in females, regardless of age, and highest in children less than 5 years old. major regional differences were detected, with the highest rates in wales and the southwest and the lowest in the southeast. the disease displa ... | 2005 | 15640174 |
| rapid detection and differentiation of pathogenic campylobacter jejuni and campylobacter coli by real-time pcr. | a two-tube real-time assay, developed in a lightcycler, was used to detect, identify and differentiate campylobacter jejuni and campylobacter coli from all other pathogenic members of the family campylobacteriaceae. in the first assay, continuous monitoring of the fluorescence resonance energy transfer (fret) signal acquired from the hybridisation of two adjacent fluoroprobes, a specific fitc probe 5'-gtgctagcttgctagaacttagaga-fitc-3') and a universal downstream probe cy5 (5'-cy5-aggtgitgcatggit ... | 2005 | 15636755 |
| sequence typing and comparison of population biology of campylobacter coli and campylobacter jejuni. | a multilocus sequence typing (mlst) scheme that uses the same loci as a previously described system for campylobacter jejuni was developed for campylobacter coli. the c. coli-specific primers were validated with 53 isolates from humans, chickens, and pigs, together with 15 penner serotype reference isolates. the nucleotide sequence of the flaa short variable region (svr) was determined for each isolate. these sequence data were compared to equivalent information for 17 c. jejuni isolates represe ... | 2005 | 15634992 |
| epidemiology of campylobacter jejuni isolated from patients with guillain-barré and fisher syndromes in japan. | campylobacter jejuni isolation is the standard for the diagnosis of this type of bacterial infection, but there have been no epidemiological studies of a large number of c. jejuni isolates from patients with guillain-barre syndrome (gbs) and fisher syndrome (fs). for 13 years, stool specimens from gbs/fs patients have been sent from 378 hospitals throughout japan to the tokyo metropolitan institute of public health. a total of 113 strains (11%) were isolated from the stool specimens from 1,049 p ... | 2005 | 15634991 |
| enhanced in vivo fitness of fluoroquinolone-resistant campylobacter jejuni in the absence of antibiotic selection pressure. | campylobacter jejuni, a major foodborne human pathogen, has become increasingly resistant to fluoroquinolone (fq) antimicrobials. by using clonally related isolates and genetically defined mutants, we determined the fitness of fq-resistant campylobacter in chicken (a natural host and a major reservoir for c. jejuni) in the absence of antibiotic selection pressure. when monoinoculated into the host, fq-resistant and fq-susceptible campylobacter displayed similar levels of colonization and persist ... | 2005 | 15634738 |
| human innate b cells: a link between host defense and autoimmunity? | b cells play a variety of immunoregulatory roles through their antigen-presentation ability and through cytokine and chemokine production. innate immune activation of b cells may play a beneficial role through the generation of natural cross-reactive antibodies, by maintaining b cell memory and by exercising immunomodulatory functions that may provide protection against autoimmunity. in this article, we review human b cell populations and their functional properties, with a particular focus on a ... | 2005 | 15633016 |
| campylobacter jejuni gene expression in response to iron limitation and the role of fur. | campylobacter jejuni is a zoonotic pathogen and the most common cause of bacterial foodborne diarrhoeal illness worldwide. to establish intestinal colonization prior to either a commensal or pathogenic interaction with the host, c. jejuni will encounter iron-limited niches where there is likely to be intense competition from the host and normal microbiota for iron. to gain a better understanding of iron homeostasis and the role of ferric uptake regulator (fur) in iron acquisition in c. jejuni, a ... | 2005 | 15632442 |
| a sulphite respiration system in the chemoheterotrophic human pathogen campylobacter jejuni. | the ability to use sulphite as a respiratory electron donor is usually associated with free-living chemolithotrophic sulphur-oxidizing bacteria. however, this paper shows that the chemoheterotrophic human pathogen campylobacter jejuni has the ability to respire sulphite, with oxygen uptake rates of 23 +/- 8 and 28 +/- 15 nmol o(2) min(-1) (mg cell protein)(-1) after the addition of 0.5 mm sodium sulphite or metabisulphite, respectively, to intact cells. the c. jejuni nctc 11168 cj0004c and cj000 ... | 2005 | 15632441 |
| characterization of new dsbb-like thiol-oxidoreductases of campylobacter jejuni and helicobacter pylori and classification of the dsbb family based on phylogenomic, structural and functional criteria. | in gram-negative bacterial cells, disulfide bond formation occurs in the oxidative environment of the periplasm and is catalysed by dsb (disulfide bond) proteins found in the periplasm and in the inner membrane. in this report the identification of a new subfamily of disulfide oxidoreductases encoded by a gene denoted dsbi, and functional characterization of dsbi proteins from campylobacter jejuni and helicobacter pylori, as well as dsbb from c. jejuni, are described. the n-terminal domain of ds ... | 2005 | 15632440 |
| neurologic aspects of infections in international travelers. | as international travel for business and pleasure becomes part of contemporary lifestyle, the clinician today is confronted with an increasing number of travelers returning ill with unfamiliar syndromes. the physician will encounter a myriad of patients with exotic infections, emerging infectious diseases, or resurgent old-world infections. | 2005 | 15631642 |
| introns in the cytolethal distending toxin gene of actinobacillus actinomycetemcomitans. | in eukaryotic cells, genes are interrupted by intervening sequences called introns. introns are transcribed as part of a precursor rna that is subsequently removed by splicing, giving rise to mature mrna. however, introns are rarely found in bacteria. actinobacillus actinomycetemcomitans is a periodontal pathogen implicated in aggressive forms of periodontal disease. this organism has been shown to produce cytolethal distending toxin (cdt), which causes sensitive eukaryotic cells to become irrev ... | 2005 | 15629928 |
| outbreaks of enteric infections caused by multiple pathogens associated with calves at a farm day camp. | transmission of enteric pathogens at venues where the public contacts farm animals is a growing problem, particularly among children. in 2000 and again in 2001, enteric illness outbreaks caused by multiple pathogens occurred at a farm day camp for children in minnesota. | 2004 | 15626945 |
| differential protein expression by porphyromonas gingivalis in response to secreted epithelial cell components. | the human oral pathogen porphyromonas gingivalis colonizes the gingival crevice and invades gingival epithelial cells. multidimensional capillary high-performance liquid chromatography coupled with tandem mass spectrometry and two-dimensional gel electrophoresis were used to analyze the proteome of p. gingivalis as it adapts to a set of experimental conditions designed to reflect important features of an epithelial cell environment. 1014 proteins (46% of the total theoretical proteome) were iden ... | 2005 | 15619293 |
| cytolethal distending toxin from shiga toxin-producing escherichia coli o157 causes irreversible g2/m arrest, inhibition of proliferation, and death of human endothelial cells. | recently, cytolethal distending toxin v (cdt-v), a new member of the cdt family, was identified in shiga toxin-producing escherichia coli (stec) o157 and particular non-o157 serotypes. here we investigated the biological effects of cdt-v from stec o157:h(-) (strain 493/89) on human endothelial cells, which are believed to be major pathogenetic targets in severe stec-mediated diseases. cdt-v caused dose-dependent g(2)/m cell cycle arrest leading to distension, inhibition of proliferation, and dea ... | 2005 | 15618195 |
| detection and quantification of macrolide resistance mutations at positions 2058 and 2059 of the 23s rrna gene by pyrosequencing. | a pyrosequencing method for detection and quantification of macrolide resistance mutations at positions 2058 and 2059 (escherichia coli numbering) of the 23s rrna gene is described. the method was developed and tested for streptococcus pneumoniae, streptococcus pyogenes, mycobacterium avium, campylobacter jejuni, and haemophilus influenzae. | 2005 | 15616336 |
| anion-independent iron coordination by the campylobacter jejuni ferric binding protein. | campylobacter jejuni, the leading cause of human gastroenteritis, expresses a ferric binding protein (cfbpa) that in many pathogenic bacteria functions to acquire iron as part of their virulence repertoire. recombinant cfbpa is isolated with ferric iron bound from escherichia coli. the crystal structure of cfbpa reveals unprecedented iron coordination by only five protein ligands. the histidine and one tyrosine are derived from the n-terminal domain, whereas the three remaining tyrosine ligands ... | 2004 | 15613474 |
| hiv+ malt lymphoma remission induced by highly active antiretroviral therapy alone. | malt lymphoma is usually described in association with helicobacter pylori, hcv, hhv8, campylobacter jejuni or in a setting of overreactive immunity. in hiv(+) patients, malt lymphoma is most commonly described in children. we describe here an original case of hiv(+) malt lymphoma with bronchial, conjuctival and laryngeal involvement for which a clinical and histopathological remission has been obtained with haart alone. we conclude that hiv, as well as h. pylori, c. jejuni and hcv can target ly ... | 2005 | 15613110 |
| analysis of campylobacter jejuni capsular loci reveals multiple mechanisms for the generation of structural diversity and the ability to form complex heptoses. | we recently demonstrated that campylobacter jejuni produces a capsular polysaccharide (cps) that is the major antigenic component of the classical penner serotyping system distinguishing campylobacter into >60 groups. although the wide variety of c. jejuni serotypes are suggestive of structural differences in cps, the genetic mechanisms of such differences are unknown. in this study we sequenced biosynthetic cps regions, ranging in size from 15 to 34 kb, from selected c. jejuni strains of hs:1, ... | 2005 | 15612919 |
| identification and characterization of a major subgroup of conjugative campylobacter jejuni plasmids. | enterocyte invasion of campylobacter jejuni 81-176 has been reported to depend upon the virulence plasmid pvir. the objective of this study was to determine the prevalence of pvir in clinical c. jejuni isolates, to investigate dna homologies between c. jejuni plasmids and the significance of plasmids for c. jejuni invasiveness. | 2005 | 15603835 |
| guillain-barre syndrome with meningoencephalitis after campylobacter jejuni infection. | a 14-year-old boy presented with progressive ascending muscle weakness, urinary retention and disturbed consciousness. initially his cerebrospinal fluid showed pleocytosis, and protein-cellular dissociation developed later. campylobacter jejuni was isolated from his stool and serum anti-ganglioside antibodies were positive. our case suggests that coexistence of meningoencephalitis at an early stage of illness does not necessarily exclude the diagnosis of guillain-barre syndrome. | 2004 | 15602203 |
| identification of a dichelobacter nodosus ferric uptake regulator and determination of its regulatory targets. | the expression of iron regulated genes in bacteria is typically controlled by the ferric uptake regulator (fur) protein, a global transcriptional repressor that regulates functions as diverse as iron acquisition, oxidative stress, and virulence. we have identified a fur homologue in dichelobacter nodosus, the causative agent of ovine footrot, and shown that it complements an escherichia coli fur mutant. homology modeling of the d. nodosus fur protein with the recently solved crystal structure of ... | 2005 | 15601721 |
| regulation of uptake and processing of the quorum-sensing autoinducer ai-2 in escherichia coli. | ai-2 is a quorum-sensing signaling molecule proposed to be involved in interspecies communication. in escherichia coli and salmonella enterica serovar typhimurium, extracellular ai-2 accumulates in exponential phase, but the amount decreases drastically upon entry into stationary phase. in s. enterica serovar typhimurium, the reduction in activity is due to import and processing of ai-2 by the lsr transporter. we show that the lsr transporter is functional in e. coli, and screening for mutants d ... | 2005 | 15601708 |
| isolated abducens nerve paresis associated with high titer of anti-asialo-gm1 following campylobacter jejuni enteritis. | 2004 | 15592739 | |
| human peripheral and gastric lymphocyte responses to helicobacter pylori napa and aphc differ in infected and uninfected individuals. | in this study, we identify the nature of the immunological response of human peripheral blood mononuclear cells (pbmc) and lamina propria gastric lymphocytes (lpl) to two helicobacter pylori antigens, the neutrophil activating protein (napa) and alkyl hydroperoxide reductase (aphc). these antigens were identified and selected for study based on the observation that serological recognition of these proteins was associated with h pylori negative status in humans. | 2005 | 15591500 |
| will worms really cure crohn's disease? | 2005 | 15591496 | |
| mitochondrial cytochrome c1 is a collapsed di-heme cytochrome. | cytochrome c(1) from mitochondrial complex iii and the di-heme cytochromes c in the corresponding enzyme from epsilon-proteobacteria have so far been considered to represent unrelated cytochromes. a missing link protein discovered in the genome of the hyperthermophilic bacterium aquifex aeolicus, however, provides evidence for a close evolutionary relationship between these two cytochromes. the mono-heme cytochrome c(1) from a. aeolicus contains stretches of strong sequence homology toward the e ... | 2004 | 15591339 |
| new insights into type ii nad(p)h:quinone oxidoreductases. | type ii nad(p)h:quinone oxidoreductases (ndh-2) catalyze the two-electron transfer from nad(p)h to quinones, without any energy-transducing site. ndh-2 accomplish the turnover of nad(p)h, regenerating the nad(p)(+) pool, and may contribute to the generation of a membrane potential through complexes iii and iv. these enzymes are usually constituted by a nontransmembrane polypeptide chain of approximately 50 kda, containing a flavin moiety. there are a few compounds that can prevent their activity ... | 2004 | 15590775 |
| crystallization and preliminary crystallographic studies of momp (major outer membrane protein) from campylobacter jejuni. | campylobacter jejuni is the leading bacterial cause of human enteritis linked to ingestion of contaminated food or water. momp, the major outer membrane protein from these gram-negative bacteria, belongs to the porin family. in order to determine the three-dimensional structure of this protein and to elucidate the underlying molecular mechanisms, the momp from c. jejuni strain 85h has been purified and crystallized by vapour diffusion. two crystal forms were characterized for this membrane prote ... | 2004 | 15583387 |
| simultaneous detection of staphylococcus aureus and coagulase-negative staphylococci in positive blood cultures by real-time pcr with two fluorescence resonance energy transfer probe sets. | a real-time pcr assay that uses two fluorescence resonance energy transfer probe sets and targets the tuf gene of staphylococci is described here. one probe set detects the staphylococcus genus, whereas the other probe set is specific for staphylococcus aureus. one hundred thirty-eight cultured isolates, which contained 41 isolates of staphylococci representing at least nine species, and 100 positive blood cultures that contained gram-positive cocci in clusters were tested. this assay was 100% s ... | 2004 | 15583307 |
| phylogenetic analysis and pcr-restriction fragment length polymorphism identification of campylobacter species based on partial groel gene sequences. | the phylogeny of 12 campylobacter species and reference strains of arcobacter butzleri and helicobacter pylori was studied based on partial 593-bp groel gene sequences. the topology of the phylogenetic neighbor-joining tree based on the groel gene was similar to that of the tree based on the 16s rrna gene. however, groel was found to provide a better resolution for campylobacter species, with lower interspecies sequence similarities (range, 65 to 94%) compared with those for the 16s rrna gene (r ... | 2004 | 15583306 |
| differentiation of campylobacter coli, campylobacter jejuni, campylobacter lari, and campylobacter upsaliensis by a multiplex pcr developed from the nucleotide sequence of the lipid a gene lpxa. | we describe a multiplex pcr assay to identify and discriminate between isolates of campylobacter coli, campylobacter jejuni, campylobacter lari, and campylobacter upsaliensis. the c. jejuni isolate f38011 lpxa gene, encoding a udp-n-acetylglucosamine acyltransferase, was identified by sequence analysis of an expression plasmid that restored wild-type lipopolysaccharide levels in escherichia coli strain sm105 [lpxa(ts)]. with oligonucleotide primers developed to the c. jejuni lpxa gene, nearly fu ... | 2004 | 15583280 |
| the crucial role of campylobacter jejuni genes in anti-ganglioside antibody induction in guillain-barre syndrome. | molecular mimicry of campylobacter jejuni lipo-oligosaccharides (los) with gangliosides in nervous tissue is considered to induce cross-reactive antibodies that lead to guillain-barre syndrome (gbs), an acute polyneuropathy. to determine whether specific bacterial genes are crucial for the biosynthesis of ganglioside-like structures and the induction of anti-ganglioside antibodies, we characterized the c. jejuni los biosynthesis gene locus in gbs-associated and control strains. we demonstrated t ... | 2004 | 15578098 |
| identification of a distinct, cryptic heparosan synthase from pasteurella multocida types a, d, and f. | the extracellular polysaccharide capsules of pasteurella multocida types a, d, and f are composed of hyaluronan, n-acetylheparosan (heparosan or unsulfated, unepimerized heparin), and unsulfated chondroitin, respectively. previously, a type d heparosan synthase, a glycosyltransferase that forms the repeating disaccharide heparosan backbone, was identified. here, a approximately 73% identical gene product that is encoded outside of the capsule biosynthesis locus was also shown to be a functional ... | 2004 | 15576804 |
| lymphoglandular complexes are important colonic sites for immunoglobulin a induction against campylobacter jejuni in a swine disease model. | campylobacter jejuni is a common cause of serious food-borne illness. in an experimental model of human infection, germfree pigs were given c. jejuni, the whipworm trichuris suis, dual infections with c. jejuni and t. suis, or no infection. in dual-infected pigs, a synergistic effect between c. jejuni and t. suis was observed 27 days after infection, when t. suis fourth-stage larvae were found in the proximal colon. in dual-infected pigs, lymphoglandular complexes (lgcs) in the distal colon were ... | 2004 | 15575364 |
| effects of climate on incidence of campylobacter spp. in humans and prevalence in broiler flocks in denmark. | campylobacter infections are increasing and pose a serious public health problem in denmark. infections in humans and broiler flocks show similar seasonality, suggesting that climate may play a role in infection. we examined the effects of temperature, precipitation, relative humidity, and hours of sunlight on campylobacter incidence in humans and broiler flocks by using lag dependence functions, locally fitted linear models, and cross validation methods. for humans, the best model included aver ... | 2004 | 15574950 |
| allelic diversity and population structure in oenococcus oeni as determined from sequence analysis of housekeeping genes. | oenococcus oeni is the organism of choice for promoting malolactic fermentation in wine. the population biology of o. oeni is poorly understood and remains unclear. for a better understanding of the mode of genetic variation within this species, we investigated by using multilocus sequence typing (mlst) with the gyrb, pgm, ddl, recp, and mlea genes the genetic diversity and genetic relationships among 18 o. oeni strains isolated in various years from wines of the united states, france, germany, ... | 2004 | 15574919 |
| survival of cold-stressed campylobacter jejuni on ground chicken and chicken skin during frozen storage. | campylobacter jejuni is prevalent in poultry, but the effect of combined refrigerated and frozen storage on its survival, conditions relevant to poultry processing and storage, has not been evaluated. therefore, the effects of refrigeration at 4 degrees c, freezing at -20 degrees c, and a combination of refrigeration and freezing on the survival of c. jejuni in ground chicken and on chicken skin were examined. samples were enumerated using tryptic soy agar containing sheep's blood and modified c ... | 2004 | 15574906 |
| characterization of lactobacillus coryniformis dsm 20001t surface protein cpf mediating coaggregation with and aggregation among pathogens. | phenotypic characterization of aggregation phenotypes of lactobacillus coryniformis revealed that strain dsm 20001t coaggregated with escherichia coli k88, campylobacter coli, and campylobacter jejuni but not with other human pathogens. in addition, cells of these pathogens aggregated in the presence of the spent culture supernatant (scs) of strain dsm 20001t. cells of e. coli k88 remained viable in the coaggregates and aggregates for up to 24 h. both coaggregation and aggregation (co/aggregatio ... | 2004 | 15574903 |
| diagnostic real-time pcr for detection of salmonella in food. | a robust 5' nuclease (taqman) real-time pcr was developed and validated in-house for the specific detection of salmonella in food. the assay used specifically designed primers and a probe target within the ttrrsbca locus, which is located near the salmonella pathogenicity island 2 at centisome 30.5. it is required for tetrathionate respiration in salmonella. the assay correctly identified all 110 salmonella strains and 87 non-salmonella strains tested. an internal amplification control, which is ... | 2004 | 15574899 |
| the n-x-s/t consensus sequence is required but not sufficient for bacterial n-linked protein glycosylation. | in the gram-negative bacterium campylobacter jejuni there is a pgl (protein glycosylation) locus-dependent general n-glycosylation system of proteins. one of the proteins encoded by pgl locus, pglb, a homolog of the eukaryotic oligosaccharyltransferase component stt3p, is proposed to function as an oligosaccharyltransferase in this prokaryotic system. the sequence requirements of the acceptor polypeptide for n-glycosylation were analyzed by reverse genetics using the reconstituted glycosylation ... | 2004 | 15574802 |
| regional risks and seasonality in travel-associated campylobacteriosis. | the epidemiology of travel-associated campylobacteriosis is still largely unclear, and various known risk factors could only explain limited proportions of the recorded cases. | 2004 | 15569393 |
| covert operations of uropathogenic escherichia coli within the urinary tract. | entry into host cells is required for many bacterial pathogens to effectively disseminate within a host, avoid immune detection and cause disease. in recent years, many ostensibly extracellular bacteria have been shown to act as opportunistic intracellular pathogens. among these are strains of uropathogenic escherichia coli (upec), the primary causative agents of urinary tract infections (utis). upec are able to transiently invade, survive and multiply within the host cells and tissues constitut ... | 2005 | 15569242 |
| evolutionary relationships of fusobacterium nucleatum based on phylogenetic analysis and comparative genomics. | the phylogenetic position and evolutionary relationships of fusobacteria remain uncertain. especially intriguing is their relatedness to low g+c gram positive bacteria (firmicutes) by ribosomal molecular phylogenies, but their possession of a typical gram negative outer membrane. taking advantage of the recent completion of the fusobacterium nucleatum genome sequence we have examined the evolutionary relationships of fusobacterium genes by phylogenetic analysis and comparative genomics tools. | 2004 | 15566569 |
| induction of cationic chicken liver-expressed antimicrobial peptide 2 in response to salmonella enterica infection. | cationic antimicrobial peptides constitute part of the innate immune system and provide an essential role in the defense against infection. at present there is a paucity of information regarding the antimicrobial profile of the chicken (gallus gallus). using in silico studies, an expressed sequence tag (est) clone was identified which encodes a novel cationic antimicrobial peptide, chicken liver-expressed antimicrobial peptide 2 (cleap-2). the predicted amino acid sequence composed a prepropepti ... | 2004 | 15557621 |
| atp-binding cassette transporters are targets for the development of antibacterial vaccines and therapies. | 2004 | 15557595 | |
| the campylobacter jejuni dccrs two-component system is required for optimal in vivo colonization but is dispensable for in vitro growth. | a campylobacter jejuni two-component signal transduction system (tcsts), designated dccr-dccs (diminished capacity to colonize; cj1223c-cj1222c), has been found to be important for in vivo colonization but dispensable for in vitro growth. a deltadccr response regulator mutant generated using the virulent strain 81-176 background exhibited significantly reduced colonization of immunocompetent limited flora (i-lf) mice, severe combined immunodeficient limited flora (scid-lf) mice, and 1-day-old ch ... | 2004 | 15554967 |
| rubrerythrin from the hyperthermophilic archaeon pyrococcus furiosus is a rubredoxin-dependent, iron-containing peroxidase. | rubrerythrin was purified by multistep chromatography under anaerobic, reducing conditions from the hyperthermophilic archaeon pyrococcus furiosus. it is a homodimer with a molecular mass of 39.2 kda and contains 2.9 +/- 0.2 iron atoms per subunit. the purified protein had peroxidase activity at 85 degrees c using hydrogen peroxide with reduced p. furiosus rubredoxin as the electron donor. the specific activity was 36 micromol of rubredoxin oxidized/min/mg with apparent k(m) values of 35 and 70 ... | 2004 | 15547260 |
| development of an extensive set of 16s rdna-targeted primers for quantification of pathogenic and indigenous bacteria in faecal samples by real-time pcr. | the microbiota of the human intestinal tract constitutes a complex ecosystem. we report the design and optimization of an extensive set of 16s rdna-targeted species- and group-specific primers for more accurate quantification of bacteria from faecal samples with real-time pcr. | 2004 | 15546407 |
| campylobacter insulaenigrae sp. nov., isolated from marine mammals. | phenotypic and phylogenetic studies were performed on four campylobacter-like organisms recovered from three seals and a porpoise. comparative 16s rrna gene sequencing studies demonstrated that the organisms represent a hitherto unknown subline within the genus campylobacter, associated with a subcluster containing campylobacter jejuni, campylobacter coli and campylobacter lari. dna-dna hybridization studies confirmed that the bacteria belonged to a single species, for which the name campylobact ... | 2004 | 15545485 |
| immunoproliferative small intestinal disease (ipsid): a model for mature b-cell neoplasms. | immunoproliferative small intestinal disease (ipsid) was recently added to the growing list of infectious pathogen-associated human lymphomas. molecular and immunohistochemical studies demonstrated an association with campylobacter jejuni. ipsid is a variant of the b-cell lymphoma of mucosa-associated lymphoid tissue (malt), which involves mainly the proximal small intestine resulting in malabsorption, diarrhea, and abdominal pain. geographically, ipsid is most prevalent in the middle east and a ... | 2005 | 15542584 |
| survival of campylobacter jejuni on various fresh produce. | the survival of seven human and two chicken campylobacter jejuni strains, with known penner heat-stable (hs) serotypes and pulsed-field gel electrophoresis (pfge) genotypes, was investigated on fresh-cut iceberg lettuce. in addition, the survival of four selected c. jejuni strains was assessed on cantaloupe pieces, cucumber slices, grated carrot and strawberries. fresh produce was inoculated with 10(5) to 10(7) colony-forming units (cfu) of c. jejuni per gram, and the bacterium was enumerated us ... | 2004 | 15541805 |
| antimicrobial resistance in campylobacter strains isolated from french broilers before and after antimicrobial growth promoter bans. | the antimicrobial susceptibility of campylobacter strains isolated from standard and free-range broilers in 1992-1996 and 2001-2002 was studied. | 2004 | 15537699 |
| a genomic timescale of prokaryote evolution: insights into the origin of methanogenesis, phototrophy, and the colonization of land. | the timescale of prokaryote evolution has been difficult to reconstruct because of a limited fossil record and complexities associated with molecular clocks and deep divergences. however, the relatively large number of genome sequences currently available has provided a better opportunity to control for potential biases such as horizontal gene transfer and rate differences among lineages. we assembled a data set of sequences from 32 proteins (approximately 7600 amino acids) common to 72 species ... | 2004 | 15535883 |
| studies on calf diarrhoea in mozambique: prevalence of bacterial pathogens. | the prevalence of diarrhoea in calves was investigated in 8 dairy farms in mozambique at 4 occasions during 2 consecutive years. a total of 1241 calves up to 6 months of age were reared in the farms, and 63 (5%) of them had signs of diarrhoea. two farms had an overall higher prevalence (13% and 21%) of diarrhoea. faecal samples were collected from all diarrhoeal calves (n = 63) and from 330 healthy calves and analysed for salmonella species, campylobacter jejuni and enterotoxigenic escherichia c ... | 2004 | 15535084 |
| dna condensation and self-aggregation of escherichia coli dps are coupled phenomena related to the properties of the n-terminus. | escherichia coli dps (dna-binding proteins from starved cells) is the prototype of a dna-protecting protein family expressed by bacteria under nutritional and oxidative stress. the role of the lysine-rich and highly mobile dps n-terminus in dna protection has been investigated by comparing the self-aggregation and dna-condensation capacity of wild-type dps and two n-terminal deletion mutants, dpsdelta8 and dpsdelta18, lacking two or all three lysine residues, respectively. gel mobility and atomi ... | 2004 | 15534364 |
| selenophosphate synthetase genes from lung adenocarcinoma cells: sps1 for recycling l-selenocysteine and sps2 for selenite assimilation. | a labile selenium donor compound monoselenophosphate is synthesized from selenide and atp by selenophosphate synthetase (sps). in the present study, sps1 and sps2 were cloned from a cdna library prepared from human lung adenocarcinoma cells (ncih441). the human lung sps1 has been cloned as an orf of 1,179 bp, identical in sequence to that of the recently revised human liver sps1. the in-frame tga codon of the lung sps2 was genetically altered to tgt (cys) to obtain the sps2cys gene. expression o ... | 2004 | 15534230 |
| [erythema nodosum: etiological changes in the last two decades]. | to study the etiological factors associated with erythema nodosum in children hospitalized between january 1985 and december 2003. | 2004 | 15530319 |
| inability of cecal microflora to promote reversion of viable nonculturable campylobacter jejuni. | campylobacter jejuni cells are able to enter a viable but nonculturable (vbnc) state when they are suspended in water. in the present experiments we inoculated day-of-hatch leghorn and broiler chicks with normal gut microflora and subsequently challenged these with high doses of vbnc c. jejuni. the objective was to determine if the pre-establishment of a normal gut flora would enable vbnc campylobacter to recover, revert to the vibrionic form, and colonize the cecum. day-of-hatch leghorn and bro ... | 2004 | 15529989 |
| sensitive and specific detection of yersinia pseudotuberculosis by loop-mediated isothermal amplification. | we developed a loop-mediated isothermal amplification method able to detect yersinia pseudotuberculosis strains in 30 min by using six primers designed by targeting the inv gene. this method is more sensitive than pcr and might be a useful tool for detecting and identifying y. pseudotuberculosis. | 2004 | 15528740 |
| detection of campylobacter spp. in chicken fecal samples by real-time pcr. | a real-time pcr assay for detecting thermophilic campylobacter spp. directly in chicken feces has been developed. dna was isolated from fecal material by using magnetic beads followed by pcr with a prealiquoted pcr mixture, which had been stored at -18 degrees c. campylobacter could be detected in less than 4 h, with a detection limit of 100 to 150 cfu/ml, in a fecal suspension. a bacterial internal control was added before dna extraction to control both dna isolation and the presence of pcr inh ... | 2004 | 15528705 |
| frequency and spatial distribution of environmental campylobacter spp. | humans are exposed to campylobacter spp. in a range of sources via both food and environmental pathways. for this study, we explored the frequency and distribution of thermophilic campylobacter spp. in a 10- by 10-km square rural area of cheshire, united kingdom. the area contains approximately 70, mainly dairy, farms and is used extensively for outdoor recreational activities. campylobacter spp. were isolated from a range of environmental samples by use of a systematic sampling grid. livestock ... | 2004 | 15528512 |
| [trends in the incidence of salmonellosis and campylobacteriosis in the czech republic]. | salmonellosis and campylobacteriosis are the most frequently reported acute enteric diseases of infectious origin in the czech republic. epidemiological data on salmonellosis and campylobacteriosis have been reportable in this country since 1951 and 1984, respectively. in 2003, 53,486 cases of acute enteric infections were reported: 26,899 (52%) diagnosed as salmonellosis and 20,063 (almost 40%) diagnosed as campylobacteriosis. in 1989, the annual incidence of salmonellosis was three times as hi ... | 2004 | 15524267 |
| crohn's disease and infections: a complex relationship. | 2004 | 15520637 | |
| global transcriptome analysis of the heat shock response of shewanella oneidensis. | shewanella oneidensis is an important model organism for bioremediation studies because of its diverse respiratory capabilities. however, the genetic basis and regulatory mechanisms underlying the ability of s. oneidensis to survive and adapt to various environmentally relevant stresses is poorly understood. to define this organism's molecular response to elevated growth temperatures, temporal gene expression profiles were examined in cells subjected to heat stress by using whole-genome dna micr ... | 2004 | 15516594 |
| effect of host species on recg phenotypes in helicobacter pylori and escherichia coli. | recombination is a fundamental mechanism for the generation of genetic variation. helicobacter pylori strains have different frequencies of intragenomic recombination, arising from deletions and duplications between dna repeat sequences, as well as intergenomic recombination, facilitated by their natural competence. we identified a gene, hp1523, that influences recombination frequencies in this highly diverse bacterium and demonstrate its importance in maintaining genomic integrity by limiting r ... | 2004 | 15516585 |
| a single bifunctional udp-glcnac/glc 4-epimerase supports the synthesis of three cell surface glycoconjugates in campylobacter jejuni. | the major cell-surface carbohydrates (lipooligosaccharide, capsule, and glycoprotein n-linked heptasaccharide) of campylobacter jejuni nctc 11168 contain gal and/or galnac residues. gale is the sole annotated udp-glucose 4-epimerase in this bacterium. the presence of galnac residues in these carbohydrates suggested that gale might be a udp-glcnac 4-epimerase. gale was shown to epimerize udp-glc and udp-glcnac in coupled assays with c. jejuni glycosyltransferases and in sugar nucleotide epimeriza ... | 2005 | 15509570 |
| detection of seven virulence and toxin genes of campylobacter jejuni isolates from danish turkeys by pcr and cytolethal distending toxin production of the isolates. | a total of 117 campylobacter jejuni isolates from danish turkeys were tested for the presence of seven virulence and toxin genes by pcr. one hundred seventeen (100%) isolates were positive for flaa, cadf, and ceue gene primers. one hundred three (88%) isolates were positive for cdt gene cluster pcr detection (cdt gene cluster-pcr), whereas 101 (86.3%), 102 (87.2%), and 110 (94%) isolates were positive for cdta-, cdtb-, and cdtc-pcr, respectively. only 39 (33.3%) isolates were positive for virb11 ... | 2004 | 15508626 |
| arcobacter species in humans. | during an 8-year study period, arcobacter butzleri was the fourth most common campylobacter-like organism isolated from 67,599 stool specimens. our observations suggest that a. butzleri displays microbiologic and clinical features similar to those of campylobacter jejuni; however, a. butzleri is more frequently associated with a persistent, watery diarrhea. | 2004 | 15504280 |
| respiratory hydrogen use by salmonella enterica serovar typhimurium is essential for virulence. | based on available annotated gene sequence information, the enteric pathogen salmonella, like other enteric bacteria, contains three putative membrane-associated h2-using hydrogenase enzymes. these enzymes split molecular h2, releasing low-potential electrons that are used to reduce quinone or heme-containing components of the respiratory chain. here we show that each of the three distinct membrane-associated hydrogenases of salmonella enterica serovar typhimurium is coupled to a respiratory pat ... | 2004 | 15501756 |
| antimicrobial resistance of campylobacter jejuni and campylobacter coli isolates in japan. | 2004 | 15499812 | |
| flies and campylobacter infection of broiler flocks. | a total of 8.2% of flies caught outside a broiler house in denmark had the potential to transmit campylobacter jejuni to chickens, and hundreds of flies per day passed through the ventilation system into the broiler house. our study suggests that flies may be an important source of campylobacter infection of broiler flocks in summer. | 2004 | 15496257 |
| swimming and campylobacter infections. | a matched case-control study was conducted to study risk factors for domestically acquired sporadic campylobacter infections in finland. swimming in natural sources of water was a novel risk factor. eating undercooked meat and drinking dug-well water were also independent risk factors for campylobacter infection. | 2004 | 15496253 |
| comparative analysis of four campylobacterales. | comparative genome analysis can be used to identify species-specific genes and gene clusters, and analysis of these genes can give an insight into the mechanisms involved in a specific bacteria-host interaction. comparative analysis can also provide important information on the genome dynamics and degree of recombination in a particular species. this article describes the comparative genome analysis of representatives of four different campylobacterales species - two pathogens of humans, helicob ... | 2004 | 15494744 |
| high-throughput computational and experimental techniques in structural genomics. | structural genomics has as its goal the provision of structural information for all possible orf sequences through a combination of experimental and computational approaches. the access to genome sequences and cloning resources from an ever-widening array of organisms is driving high-throughput structural studies by the new york structural genomics research consortium. in this report, we outline the progress of the consortium in establishing its pipeline for structural genomics, and some of the ... | 2004 | 15489337 |
| campylobacter jejuni in guillain-barré syndrome. | 2004 | 15488456 | |
| intestinal malignancy and campylobacter jejuni. | 2004 | 15481007 | |
| microbiological status of piggery effluent from 13 piggeries in the south east queensland region of australia. | to assist in the development of safe piggery effluent re-use guidelines by determining the level of selected pathogens and indicator organisms in the effluent ponds of 13 south-east queensland piggeries. | 2004 | 15479402 |
| reiter's syndrome--a case report and review of literature. | the occurrence of reiter's syndrome is rare and not commonly reported in nigeria. this paper reports a case of a 35 yr old male nigerian with reiter's syndrome, occurring 1-2 weeks after a bout of a dysenteric illness. the patient presented with fever, conjunctivitis, dysentery, urethritis and arthralgia. the joint pains involved the left wrist (which was swollen), the right knee and ankle joints. the patient was managed conservatively. the case is presented with a view to documenting the occurr ... | 2004 | 15477194 |