Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| sensitivity of chemically treated spores of clostridium perfringens type a to an initiation protein. | extraction of clostridium perfringens type a spores with dithiothreitol (dtt), dtt plus sodium dodecyl sulphate (dtt-sds), urea-mercaptoethanol (ume), or alkali, solubilized from 18.6 to 46.5 of the total dry weight of spores. the initiation of germination and lysis of such treated spores with lysozyme and an initiation protein (ip) from the culture supernatant fluid of sporulating cells of c. perfringens was studied under various conditions. the ability of lysozyme and the crude ip to induce ge ... | 1979 | 232233 |
| [assay for enterotoxin in fecal specimens of clostridium perfringens food poisoning (author's transl)]. | 1979 | 232119 | |
| enterotoxin production by lecithinase-positive and lecithinase-negative clostridium perfringens isolated from food poisoning outbreaks and other sources. | 1979 | 232099 | |
| [acute renal failure with hemolytic crisis as a complication of septic abortion]. | 1979 | 232049 | |
| icmsf methods studies. xii. comparative study for the enumeration of clostridium perfringens in feces. | as the second phase of an international comparative study for the enumeration of clostridium perfringens, four methods were compared for "total" and spore counts of c. perfringens in fecal specimens: the sfp (shahidi-ferguson perfringens) agar (a), tsc (tryptose-sulfite-cycloserine) agar (b), sc (sulfite-cycloserine) agar (c), and neomycin blood agar (d) methods. in both the total and spore count procedures, the confirmed c. perfringens counts in method d were lower than in methods a, b, and c. ... | 1979 | 232005 |
| [association of acquired polyagglutinabilities of types t and b. an observation]. | although t and acquired b polyagglutinabilities are not exceptional, simultaneous occurence of the two types is a rarer phenomenon. ten days after an open heart surgery operation, the patient, age 55, had an infectious syndrom : two strains, clostridium perfringens and peptococcus variabilis were isolated. simultaneously, her red cells were found to be polyagglutinable. the association of t and acquired b polyagglutinabilities could be demonstrated by serological studies of the patient's red cel ... | 1979 | 231812 |
| development, preparation and safety testing of a clostridium welchii type c toxoid. ii. laboratory evaluation of potency. | the results obtained with four laboratory tests on four candidate formulations of clostridium welchii type c vaccine for use in man have been compared with clinical responses to the same vaccines. quantal response assays in mice appeared to reflect the ranking of the four vaccines in human subjects better than did the guinea pig tests. they also enabled the potency of the vaccine preparations to be related to an existing international reference preparation. mouse assays in which the animals rece ... | 1979 | 231607 |
| development, preparation and safety testing of a clostridium welchii type c toxoid. i: preliminary observations in man in papua new guinea. | the reactogenicity and immunogenicity of various strengths of plain and adsorbed cl. welchii type c toxoid have been evaluated in laboratory tests and in man in papua new guinea. the greater antigenicity and acceptable clinical reactivity of a vaccine containing 50 total combining power units per 0.5 mm of adsorbed toxoid resulted in its selection for further field studies. | 1979 | 231605 |
| synthesis of choline and ethanolamine phospholipids with thiophosphoester bonds as substrates for phospholipase c. | spectrophotometric assays of esterases are sensitive, rapid, and quite specific when thioester substrates are used. glycerophospholipids with thiophosphoester bonds may be useful as substrates for phospholipase c (ec 3.1.4.3). these have been made from mercaptoglycerol and mercaptoethanol. the thiols were oxidized to disulfides, acylated, and reduced with dithiothreitol. phosphocholine derivatives were made by the classical methods for oxyphosphoesters. the phosphatidyl choline analogue was conv ... | 1979 | 231487 |
| acetamide--a metabolite of metronidazole formed by the intestinal flora. | 1979 | 231450 | |
| revival of pathogenicity of axenically grown entamoeba histolytica for the rat. | 1979 | 231423 | |
| [gas gangrene--35 years are injury from a shell splinter]. | 1979 | 231352 | |
| changes in the fecal flora of patients with crohn's disease during treatment with metronidazole. a preliminary report. | six patients with crohn's disease and three healthy volunteers were treated with metronidazole. before and during metronidazole treatment quantitative and qualitative studies of the aerobic and anaerobic fecal bacterial flora were performed, and the minimal inhibitory concentration (mic) values for metronidazole of the isolated bacteria were determined. there was no significant change in the fecal flora of the volunteers during metronidazole treatment, but three patients who clinically responded ... | 1979 | 231297 |
| immunosuppression in toxoplasmosis: studies in mice with a clostridial vaccine and louping-ill virus vaccine. | 1979 | 231055 | |
| comparison of stomacher and waring blendor for homogenizing foods to be examined for clostridium perfringens. | the colworth stomacher model 400 homogenizer was compared with the waring blendor for preparing food homogenates to be examined for clostridium perfringens. forty-eight samples representing 6 different food types were inoculated with c. perfringens and examined by the aoac official first action method for enumeration of c. perfringens in foods. identical paired specimens of each food type were blended with the 2 devices, and plate counts were made as specified in the official first action method ... | 1979 | 231030 |
| pigbel, cholera and infant botulism--a new paradigm for gut disease. | 1979 | 230800 | |
| microbiology of bruised tissue. | no significant differences could be found in the microbial quality of bruised and unbruised tissue provided that the two types of tissue were treated identically. this suggests that there is no good reason for the condemnation of bruised tissue, which could well be used in manufactured products. | 1979 | 230787 |
| [purification of clostridium perfringens type a toxins by deae-ion exchanger and gel filtration]. | 1979 | 230665 | |
| [contribution to gas gangrene of the gallbladder]. | 1979 | 230664 | |
| role of bacterial growth rates in the epidemiology and pathogenesis of urinary infections in women. | the mean minimum generation time in shake culture in urine of 6 urinary isolates of escherichia coli (21.7 +/- 0.6 min) was significantly shorter (p = 0.0003) than that of 14 isolates of less common urinary pathogens (46.0 +/- 18.6 min). mixed populations of approximately equal numbers of e. coli cells paired with other urinary, fecal, and urethral organisms were introduced into a laboratory model of the lower human urinary tract. this model used urine as a medium and reproduced some features of ... | 1979 | 230198 |
| clostridium perfringens cultured from a hawaiian sardine, sardinella marquesensis. | 1979 | 230166 | |
| biological behaviour of clostridium perfringens type a strains. | 1979 | 230152 | |
| binding of platinum(ii) intercalation reagents to deoxyribnonucleic acid. dependence on base-pair composition, nature of the intercalator, and ionic strength. | the dna binding of three platinum(ii) intercalation reagents has been studied and found to depend upon base composition, the nature of the intercalator, and the ionic strength of the solvent medium. in 0.2 m nacl, binding data for calf thymus dna show the association constants to be approximately 10(4) m-1. the binding constants decrease in the order [(o-phen)pt(en)]2+ greater than or equal to [(terpy)pt(het)]+ greater than [(bipy)pt(en)]2+. the number of available intercalation sites for the do ... | 1979 | 229900 |
| calcium-independent and dependent steps in action of clostridium perfringens enterotoxin on hela and vero cells. | 1979 | 229852 | |
| [conditions for c1. perfringens type a enterotoxin formation in vitro]. | 1979 | 229663 | |
| [use of a selective sulfite-reducing medium for the isolation of clostridium perfringens]. | the effect of sulphite-reduction in 33 sample bacterial strains was tested. with regard to the capacity of reducing sulphite in modified sulphite-reduction media in a wide scale of bacterial strains the possibility of an application of selective media with an addition of various concentrations of antibiotic solutions was checked. a concentration of 750 microgram of d-cycloserine per 1 ml of the sulphite-reduction medium appeared to be the most advantageous for the isolation and detection of sul ... | 1979 | 229618 |
| ring formation of perfringolysin o as revealed by negative stain electron microscopy. | 1979 | 228722 | |
| [studies of necrotizing enteritis of suckling piglets (cl. perfringens typc c enterotoxemia) in industrialized sow breeding units. 3. experimental reproduction of the disease]. | experimental reproduction of necrotising enteritis of sucking pigs was successfully achieved by using both clostridium perfringens type c strains, which had been isolated from sucking pigs with necrotising enteritis, and type c strain 3628 of a.t.c.c. (sub-type c1). the lethal dose for sucking pigs was between 20 x 10(6) and 12 x 10(7) pathogens per animal. the disease could not even be induced by repeated application of no-bacterial toxin of cl. perfringens type c nor by administration of cl. p ... | 1979 | 228629 |
| [presence of antibodies in human colostral secretory iga against enteric commensal bacteria: biological implications (author's transl)]. | antisecretory component, anti-alpha, anti-mu and anti-fc (gamma) fluorescent antibodies were used to detect the presence of immunoglobulins with antibody activity against enteric commensal bacteria in human colostrum and serum. forty nine colostrum samples were studied; all of them displayed secretory iga (siga) antibodies reacting with bacteroides thetaiotaomicron, clostridium perfringens and escherichia coli serotype o141:h32 without any k antigen. the amount of siga antibodies was always rela ... | 1979 | 228579 |
| [preparation and evaluation of immunogenic properties of clostridium perfringens type a toxoid]. | 1979 | 228130 | |
| spore lytic enzyme released from clostridium perfringens spores during germination. | the exudate of fully germinated spores of clostridium perfringens was found to contain a large amount of a spore lytic enzyme which acted directly on alkali-treated spores of the organism to cause germination. although no detectable amount of the enzyme was found in dormant spores during germination in a kcl medium, the enzyme was produced rapidly and released into the medium. the optimal conditions for enzyme activity were ph 6.0 and 45 degrees c. maximum activity occurred in the presence of va ... | 1979 | 227836 |
| effect of o-sulphate groups in lactose and n-acetylneuraminyl-lactose on their enzymic hydrolysis. | 1. lactose 6'-o-sulphate, n-acetylneuraminyl-(alpha 2 leads to 3)-d-lactose 6'-o-sulphate, n-acetylneuraminyl ?-o-sulphate-(alpha 2 leads to 3)-d-lactose 6'0-o-sulphate, n-acetylneuraminyl ?-o-sulphate-(alpha 2 leads to 6)-d-lactose and n-acetylneuraminyl-(alpha 2 leads to 3)- and -(alpha 2 leads to 6))-lactose 6'-o-sulphate were prepared by chemical sulphation of lactose, n-acetylneuraminyl-lactose and tis isomers by using pyridine-so3 reagent. 2. significant kinetic differences were observed i ... | 1979 | 227364 |
| neuraminidase inhibition by chemically sulphated glycopeptides. | chemically sulphated glycopeptides (derived from pig duodenal mucosa) inhibited clostridium perfringens neuraminidase (ec 3.2.1.18) activity in a ph-dependent manner. analysis of inhibition kinetics data indicated that, although the enzyme inhibition could not be categorized into any of the classical types of inhibition, it could be interpreted as a function of the size and shape of the substrates used. the enzyme activity was inhibited by 86% and 40% when tested with bovine submaxillary-gland m ... | 1979 | 227363 |
| [clostridium perfringens as a foodstuff poisoner--recommendations for the bacteriological examination of foodstuffs for clostridium perfringens]. | 1979 | 227184 | |
| [clostridium perfringens infection following cholecystectomy]. | 1979 | 226896 | |
| [acute emphysematous cholecystitis]. | emphysematous cholecystitis is a rare form of acute cholecystitis, characterized radiographically by the presence of gas within the gallbladder. we report of a patient, who was admitted to the hospital with the diagnosis of acute abdomen. this patient had an emphysematous cholecystitis caused by clostridium perfringens. we found the wall of the gallbladder emphysematous and gangrenous, the gallbladder was distended and contained purulent material, but no stones. however, in addition, the films o ... | 1979 | 226505 |
| [gas gangrene following open injuries]. | 1979 | 226502 | |
| [gas gangrene (principles of treatment, results)]. | 1979 | 226501 | |
| raffinose increases sporulation and enterotoxin production by clostridium perfringens type a. | replacement of starch with raffinose in duncan and strong sporulation medium improved percent sporulation in six of eight strains tested. enterotoxin concentration in cell extracts was increased in the case of four of five known enterotoxin-positive strains. with strain nctc 10240, levels of 0.3, 0.4, and 0.5% raffinose produced the highest enterotoxin concentration 300 to 320 micrograms of enterotoxin per mg of cell extract protein. at a level of 0.4% raffinose the highest enterotoxin concentra ... | 1979 | 225991 |
| effect of heat treatment on the performance of tryptose-sulfite-cycloserine agar for enumeration of clostridium perfringens. | dissolving dehydrated tryptose-sulfite-cycloserine agar by only boiling or microwaving was found to inhibit clostridium perfringens colony development in pour plates when compared with c. perfringens recovery in tryptose-sulfite-cycloserine agar prepared by autoclaving. | 1979 | 225988 |
| a simplified assay for phospholipase c. | 1979 | 225963 | |
| [bivalent emulsified anatoxin against ovine infectious enterotoxemia and anaerobic dysentery in lambs]. | 1979 | 225858 | |
| antibody against clostridium perfringens type a enterotoxin in healthy beef cattle and human populations in brazil. | 1978 | 225770 | |
| factors influencing the prevalence of clostridium perfringens type a in zebu beef cattle in the states of minas gerais and goias, brazil. | 1978 | 225769 | |
| prevalence of clostridium perfringens type a in two groups of food handlers in belo horizonte, minas gerias, brazil. | 1978 | 225768 | |
| [mass food poisoning of bacterial etiology in poland in the years 1973-1976]. | 1979 | 225759 | |
| high yields of coatless spores of clostridium perfringens strain 8--6 in a defined medium. | coatless spores of mutant strain 8--6 of clostridium perfringens were formed reproducibly at levels exceeding 10(7)/ml in a defined medium. confirming previous observations, the spores are quite stable in water and resistant to heat, octanol, and ethanol. they also resist 5% phenol. electron microscopy confirms, with further evidence, the probable absence of any structure exterior to the cortex. the levels of sporulation attained are sufficient to permit recovery and purification for future stud ... | 1979 | 224996 |
| antibacterial effect of some combinations of pesticides of pure cultures of microorganisms. | the investigations conducted showed that the antibacterial effect of dinitro-o-cresol, cuprozane, and trichlorfon is due to their chemical structure and to the species of microorganisms on which they act. dinitro-o-cresol and cuprozane possess selective toxicity with respect to b. anthracis and rh. gracilis and cuprozane with respect to ch. perfringens. the method of evaluating the effect of combinations of chemical compounds consisting of two or more components which have been developed made it ... | 1978 | 224955 |
| clostridia discitis: a case report. | 1979 | 224886 | |
| molecular mechanism of binding of pyrrolo(1,4)benzodiazepine antitumour agents to deoxyribonucleic acid--anthramycin and tomaymycin. | 1979 | 224883 | |
| occurrence of anaerobic bacteria in diseases of the dog and cat. | a survey for anaerobic bacteria was conducted in 314 clinical specimens from dogs and cats. a total of 187 anaerobic isolates in pure and mixed culture were isolated from 111 of the specimens that contained anaerobic bacteria. common isolated included actinomyces (9.1%), clostridium perfringens (19.3%), other clostridium spp (11.2%), peptostreptococcus anaerobius (7.5%), bacteroides melaninogenicus (13.4%), other bacteroides spp (17.6%), and fusobacterium necrophorum (5.3%). anaerobic bacteria w ... | 1979 | 224739 |
| microbiology problem. | 1979 | 224702 | |
| [approbation of nutrient media made of dry components for the accelerated cultivation of cl. perfringens type a]. | 1979 | 224626 | |
| pathogenesis of hobbs' heat-sensitive spore forming clostridium perfringens type a strain. | food poisoning in man due to heat-sensitive strains of cl. perfringens type a appeared to be mediated through enterotoxin synthesized in vivo during sporulation. a minimum of 2.0 x 10(5) vegetative cells suspended in sporulation medium was sufficient to elicit gut-loop response in rabbits. the functional disturbance in the gut as well as the structural changes were progressive and proportional to the size of the inoculum up to a dose limit of 2.0 x 10(7) vegetative cells and beyond this the chan ... | 1979 | 224284 |
| emphysematous cystitis due to clostridium perfringens in a non-diabetic dog. | 1979 | 224265 | |
| clostridium perfringens exotoxins. vi. reactivity of perfringolysin o with thiol and disulfide compounds. | the reactivity of perfringolysin o with thiol and disulfide compounds was studied. the activation potency of thiols was roughly proportional to the reaction rate constants of 5,5'-dithiobis-(2-nitrobenzoic acid) with thiols, which should be inversely proportional to their oxidation-reduction potentials. 1,2-dimercaptoethane, which had the highest rate constant, most potently activated the toxin among the thiols tested and 4,4'-dipyridyl disulfide, which is known to be one of the most potent thio ... | 1979 | 224228 |
| reverse phase passive haemagglutination and single radial immunodiffusion to detect epsilon antigen of clostridium perfringens type d. | two in vitro immunological assays were developed for detection of the epsilon (epsilon) antigen of cl. perfringens type d. it was found that the reverse phase passive haemagglutination assay (rpha) was able to detect concentrations of epsilon-antigen as low as 6 x 10-7 mg/ml whereas the single radial immunodiffusion techniques (srid) was capable of detecting concentrations of epsilon-antigen above 0.01 mg/ml. when applied to gut contents from freshly dead infected sheep the rpha test was found t ... | 1978 | 223537 |
| [demonstration and significance of gas-gangrene pathogens within the scope of hospital hygiene insepctions]. | 1979 | 223340 | |
| investigation of the immune response to aerobic and anaerobic intestinal bacteria in a patient with crohn's disease. | the immune response to aerobic and anaerobic intestinal bacteria in a patient with crohn's disease with an intestinal fistula was investigated with various serological techniques. two aerobic bacterial species, e. dispar and p. mirabilis, and four strict anaerobic bacterial species, b. fragilis ss. fragilis, f. varium and two different strains of c. perfringens, were isolated from fistula secretion of the patient. these strains were used as antigens for tube agglutination, passive hemagglutinati ... | 1979 | 223235 |
| the effects of clostridium perfringens enterotoxin on morphology, viability, and macromolecular synthesis in vero cells. | vero (african green monkey kidney) cells grown in tissue culture monolayer were sensitive to clostridium perfringens enterotoxin. within 30 minutes of exposure to the enterotoxin gross morphological damage was observed and within 40 minutes approximately 75% of the cells had detached. nearly half of the cells were nonviable following 35 to 40 minutes incubation with the enterotoxin. doses as low as 0.1 ng caused small but detectable inhibition of plating efficiency of the cells while more than 1 ... | 1979 | 222780 |
| the incidence and serotypes of clostridium perfringens on beef, pork and lamb carcasses. | 1979 | 222723 | |
| contamination of the deep tissues of carcasses by bacteria present on the slaughter instruments or in the gut. | 1979 | 222722 | |
| experimental reproduction of neonatal diarrhea in young gnotobiotic hares simultaneously associated with clostridium difficile and other clostridium strains. | clostridium difficile, c. perfringens, and c. tertium are very often present simultaneously in the feces of conventional diarrheic young hares, whereas these three bacterial species are rarely encountered and never present simultaneously in the feces of healthy young hares. when a strain of each of the three bacterial species was monoassociated with axenic young hares, the appearance of pathological disorders was only observed in animals monoassociated with c. difficile, when the number of c. di ... | 1979 | 222683 |
| clostridium perfringens septic arthritis. report of a case and review of the literature. | this is a report of clostridium perfringens septic arthritis in a 5-year-old boy and a review of the literature on 6 previously reported cases. the treatment is the same as for other organisms producing septic arthritis. | 1979 | 222526 |
| alteration of human erythrocyte plasma membranes by perfringolysin o as revealed by freeze-fracture electron microscopy. studies on clostridium perfringens exotoxins v. | when human erythrocyte membranes were treated with perfringolysin o (clostridium perfringens theta-toxin) and examined by electron microscopy after freeze-fracture, two ultrastructural alterations were observed in fracture faces of membrane. (1) a random aggregation of intramembranous particles was seen in the fracture face of the protoplasmic half (pf face) of all membranes treated with the toxin, even if at a low concentration (40 hemolytic units/ml). on the other hand, the aggregation in the ... | 1979 | 222322 |
| accessibility of sialo components in a murine tumor cell to extracellular n-acetylneuraminate glycohydrolase (sialidase). | lipid-bound sialic acid in the murine melanoma cell is not totally inaccessible to an exogenous macromolecular probe, as formerly believed. roughly 30% of the dialic acid bound to lipid, and an equal proportion of the sialic acid bound to protein is cleaved by the action of clostridium perfringens n-acetylneuraminate glycohydrolase (neuraminidase, sialidase) when the purified enzyme is added to the suspenion medium of intact murine melanoma cells freshly derived from the tumor. cleavage of lipid ... | 1979 | 222320 |
| survival of bacteria in carcasses. | bacteria injected into the bloodstream of guinea pigs shortly before death decreased in number in carcass tissues for about 1 h after death. if initial bacterial numbers were sufficiently low, all bacteria were eliminated, and carcass tissues were sterile 24 h after death. carcass tissue sterility was maintained with an initial density of clostridium perfringens or salmonella typhimurium of 20 cells per g or with an initial density of the other species examined of several hundred cells per gram. ... | 1979 | 222211 |
| [method of isolating cl. perfringens]. | 1979 | 222044 | |
| concomitant t- and tk-activation associated with acquired-b antigens. | three patients with acquired-b antigens are reported whose red blood cells also manifested t- and tk-activation. the results of tests using purified arachis hypogaea and bandeiraea simplicifolia (bs ii) lectins, and red blood cells either t, tk or both t and tk-activated by bacterial enzymes in vitro, suggest that the acquired-b phenomenon, t-activation and tk-activation result from the action of different bacterial enzymes. | 1979 | 222015 |
| clostridia as pathogens in neonatal necrotizing enterocolitis. | 1979 | 221640 | |
| binding versus biological activity of clostridium perfringens enterotoxin in vero cells. | 1979 | 220976 | |
| [preventive isolation of clostridium perfringens in food]. | 1978 | 220910 | |
| [effect of intravenous injections of clostridium perfringens (welchii) spore (entero) toxin in the horse]. | 1978 | 220692 | |
| [immunofluorescence study of clostridium perfringens sporulation and enterotoxin formation]. | 1978 | 220691 | |
| [characterization of a protein fraction that chromatographically precedes the clostridium perfringens toxin spore]. | 1978 | 220690 | |
| characterization and transferability of clostridium perfringens plasmids. | 1977 | 220651 | |
| metronidazole bioassay with increased sensitivity. | the bioassay of metronidazole using clostridium butyricum incorporated in agar plates detected concentrations higher than 1.0 microgram/ml. gentamicin in the sample did not affect the growth of the target organism or the inhibition by metronidazole. penicillin in the sample could be eliminated by the incorporation of penicillinase in the agar or by using as the target organism a surface inoculum of the penicillin-resistant bacteroides fragilis. increasing duration and temperature of aerobic pred ... | 1979 | 220515 |
| [prevention of endogenous anaerobic infection after combined operative interventions in complicated chronic pancreatitis]. | 1979 | 220460 | |
| ornidazole and anaerobic bacteria: in vitro sensitivity and effects on wound infections after appendectomy. | the sensitivities of 68 clinical isolates of bacteroides fragilis, 18 of clostridium perfringens, and 11 of other clostridium species were tested against ornidazole alone and in combination with ampicillin and gentamicin. a concentration of 3.1 microgram of ornidazole/ml inhibited 98% of the strains of b. fragilis, with greater sensitivity when ampicillin and gentamicin were also present. a concentration of 6.2 microgram of ornidazole/ml inhibited 16 of 18 strains of c. perfringens and all 11 st ... | 1979 | 220342 |
| intestinal strangulation in germfree and monocontaminated dogs. | earlier studies in the germfree rat demonstrated that the common intestinal bacteria vary greatly in their ability to cause death after intestinal strangulation. some of these experiments were repeated in adult, germfree and monocontaminated beagles. neither short closed-loop hemorrhagic nor long closed-loop ischemic strangulation killed the germfree dog. either procedure rapidly killed the dog with a conventional bacterial flora or a dog monocontaminated with clostridium perfringens. the dogs m ... | 1979 | 219816 |
| [necrotizing enteritis in suckling pigs (clostridium perfringens type c enterotoxemia). ii. toxin formation, heat and drug resistance of clostridium perfringens strains isolated from suckling pigs and broilers with necrotizing enteritis]. | nineteen clostridium perfringens type c strains and ten foreign control strains of subtypes c1, c3, and c4 were tested for their toxin formation and spore resistance to heat. the 19 type c strains had been isolated from unweaned piglets in the context of necrotising enteritis outbreaks in the gdr. the clostridium perfringens type c strains formed beta-toxin, but they failed to form epsilon-toxin or gamma-toxin, alpha-toxin was successfully recorded from 15 of the 19 strains tested from unweaned ... | 1978 | 219797 |
| anaerobic infections. | 1979 | 219788 | |
| fluid accumulation in mouse ligated intestine inoculated with clostridium perfringens enterotoxin. | clostridium perfringens enterotoxin, when inoculated into the ligated intestinal loop of mice, caused marked distension due to fluid accumulation. the increase in weight of the intestinal loop was proportional to the log dose of enterotoxin within a range from 1 to 16 micrograms. the fluid accumulation was arrested by washing the loop with saline or by injection of the specific anti-enterotoxin serum into the loop 5 or even 30 min after inoculation of the enterotoxin. a significant increase in w ... | 1979 | 219772 |
| [development of an accelerated method of determining the antibiotic sensitivity of cl. perfringens type a]. | an express method for determination of antibiotic sensitivity in the strains of cl. perfringens of type a using soviet dry nutrient media and antibiotics is proposed. the criteria for estimation of the level of the antibiotic sensitivity of the causative agent of gas gangrene in short periods on the basis of comparison of the data of the antibiotic agar diffusion procedure and the antibiotic mic were worked out. twelve antibiotics and 45 collection strains of cl. perfringens of type a were used ... | 1979 | 219770 |
| identification of a transferable tetracycline resistance plasmid (pcw3) from clostridium perfringens. | 1978 | 219433 | |
| [a prerequisite for the enterotoxin production in the rabbit ileal loop by viable cells of clostridium perfringens type a (author's transl)]. | 1979 | 219113 | |
| studies on nitrate reductase of clostridium perfringens. ii. purification and some properties of ferredoxin. | a ferredoxin was purified from clostridium perfringens by deae-cellulose chromatography and sephadex g-50 gel filtration. it had absorption maxima at 390 and 280 nm. the molecular weight was estimated to be 6,000 by sephadex gel filtration and from the results of amino acid analysis. the isoelectric point was 3.0. it contained four atoms of iron, four atoms of labile sulfur, and six cysteine residues. this ferredoxin as well as ferredoxin from c. pasteurianum acted as an electron donor for nitra ... | 1979 | 218925 |
| alteration in ultrastructure and germination of clostridium perfringens type a spores following extraction of spore coats. | 1978 | 218713 | |
| identification of enterotoxigenic clostridium perfringens type a in mixed cultures. | three known enterotoxigenic clostridium perfringens type a strains were mixed in various combinations with three nonenterotoxigenic strains and three lots of animal intestinal contents. they were grown as mixed cultures and tested for the presence of enterotoxin by the fluorescent antibody, reversed passive hemagglutination and immunodiffusion techniques. the fluorescent antibody and reversed passive hemagglutination tests detected enterotoxin in all 16 cultures prepared but the immunodiffusion ... | 1979 | 218711 |
| clostridium perfringens corneal ulcer. | a corneal ulcer caused by clostridium perfringens developed in a 76-year-old woman with sjögren's syndrome. experimental c perfringens keratitis was induced in rabbits by the intrastromal injection of 10(7) organisms. in both our patient and the experimental animals, a bullous lesion overlay the affected area of the cornea. this may be a specific lesion in clostridial infections of the cornea. clostridium perfringens should be regarded as an opportunistic corneal pathogen, and anaerobic cultures ... | 1979 | 218542 |
| mechanism of aminoglycoside antibiotic resistance in anaerobic bacteria: clostridium perfringens and bacteroides fragilis. | cell-free amino acid incorporation using ribosomes from strains of either clostridium perfringens or bacteroides fragilis was shown to be susceptible to inhibition by streptomycin and gentamicin. ribosomes bound dihydrostreptomycin as effectively as ribosomes from escherichia coli. no inactivation of streptomycin or gentamicin was detected by cell extracts of either anaerobic bacterial species. b. fragilis, grown without added hemin, menadione, and fumarate, and c. perfringens did not show any t ... | 1979 | 218500 |
| low trimethoprim susceptibility of anaerobic bacteria due to insensitive dihydrofolate reductases. | all the 28 bacteroides fragilis strains investigated were susceptible to sulfamethoxazole (minimal inhibitory concentration < 16 mug/ml) and resistant to trimethoprim (tmp; minimal inhibitory concentration > 4 mug/ml). synergism between sulfamethoxazole and tmp was present in all strains at a ratio of 1:1. the few clostridia investigated proved more resistant to both compounds. dihydrofolate reductases from b. fragilis, c. perfringens, and some other anaerobic species were isolated. inhibition p ... | 1979 | 218496 |
| [electron microscopic study of several features of spore formation in type b clostridium perfringens]. | the authors carried out electron microscopy of the thin sections of cl. perfringens, type b (strain no. 89). material of middle electron density was revealed on the cell wall surface from the first hours of the culture growing; the cytoplasm displayed both rod-like incorporations with transverse striations, and phage particles. different spore formation disturbances were revealed in the strain under study. in the majority of cells spore formation was blocked at the iii--v stage. besides, there w ... | 1979 | 218402 |
| [observations on experimentally induced gas gangrene in rats]. | 1978 | 218181 | |
| production of toxic enzymes of clostridium perfringens bp6k on technologically important media including a medium with tween 80. | the addition of 0.05--0.1% of tween 80 to casein hydrolysate medium of adams and hendee raises and production of clostridium perfringens bp6k lecithinase by approximately 50%. the use of the silicon antifoam lukosan makes the medium particularly suitable for cultivation in large volumes. higher levels of theta haemolysin and lecithinase are formed on the casein hydrolysate medium than on the meat medium. all factors except the theta and lambda components are farily stable during cultivation. | 1978 | 217931 |
| specificity of action of neuraminidase, according to its bacteriological origin. | neuraminidase from vibrio cholerae selectively cleaves sialic acid from fsh, but leaves the lh sialic acid, not influencing the biological activity of the latter hormone. on the other hand, neuraminidase from clostridium perfringens does not possess this specific action and destroys the biological activity of lh as is suggested by parlow's oaad-test. | 1978 | 217679 |
| comparative study of ten bacteriocins of clostridium perfringens. | bacteriocins of clostridium perfringens were prepared by ammonium sulfate precipitation of supernatant broth from 10 bacteriocinogenic strains. these bacteriocins were compared with respect to their ability to produce spheroplasts in a sensitive indicator strain; their inducibility; sensitivity to ph, proteolytic enzymes, and boiling; and their effect on macromolecular synthesis. two bacteriocins were stable over a wide range of ph values and resisted boiling, and three bacteriocins were resista ... | 1978 | 217302 |
| gastroenteritis: etiology, pathophysiology and clinical manifestations. | the infectious etiologic agents of gastroenteritis are manifold and have only recently been studied in greater depth. part of the problem in studies pertaining to diarrhea is the difficulty in identifying fecal "pathogens" among an enormous number of indigenous fecal microorganisms. finally, the recognition of the pathogenicity of enterotoxins has created an awareness of the complexity of gastrointestinal physiology with host-parasite interactions. | 1979 | 216878 |
| [microbiological and immunological indices in patients with peritonitis]. | 1979 | 216845 | |
| effect of silver nitrate application on the conjunctival flora of the newborn: and the occurrence of clostridial conjunctivitis. | newborn conjunctival cultures were obtained from 35 babies prior to silver nitrate application and 48 hours later. on initial culture, 46 facultative bacteria and 27 anaerobes were recovered; 48 facultative and 18 anaerobes were recovered after 48 hours. haemophilus vaginalis, bacteroides species and anaerobic cocci decreased in numbers, whereas s. epidermidis, micrococcus and propionibacterium acnes increased during this time interval. clostridial species were isolated from two cases who develo ... | 1978 | 216788 |