Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| [effect of substituted isatinisothiosemicarbazones on the multiplication of bacteria]. | 1977 | 416604 | |
| [methods of demonstrating antibiotics residues in meat, organs and eggs]. | 1977 | 416557 | |
| [effect of a bacillus cereus 504 culture on the development of homologous strain cells]. | 1978 | 416328 | |
| [a case of acute myelogenous leukemia, complicated with bacillus cereus septicemia (author's transl)]. | 1977 | 416149 | |
| prevalence of bacillus cereus in the faeces of healthy adults. | in a survey designed to determine the prevalence of bacillus cereus in the faeces of healthy persons, the organism was found in low numbers in 100 (14%) of single faecal specimens from 711 adults in the general population. in addition, in an attempt at assessing the changes in the b. cereus distribution within the faecal flora of the individual, weekly faecal specimens were submitted over a seven-week period by 18 members of staff of two laboratories. the total isolation rate was again 14%, with ... | 1978 | 416141 |
| induction of penicillinase by bacitracin. | 1978 | 415741 | |
| reversible inhibitors of penicillinases. | reversible competitive inhibitors of a penicillinase, beta-lactamase 1 from bacillus cereus, were studied. these represent the first inhibitors of a penicillinase that lack the beta-lactam ring. the products of the enzymic reaction, namely penicilloic acids, are inhibitors; their decarboxylation products, the penilloic acids, are also inhibitors, and have somewhat lower ki values. inhibitors have been prepared from benzylpenicillin, phenoxymethyl-penicillin, methicillin (2,6-dimethoxybenzamidope ... | 1978 | 415738 |
| [germination and outgrowth of spores and proliferation of outgrown vegetative cells in the peritoneal cavity of mice intraperitoneally injected with bacillus cereus or bacillus subtilis spores (author's transl)]. | 1977 | 415160 | |
| bacteriocin and antibiotic resistance plasmids in bacillus cereus and bacillus subtilis. | a number of plasmids have been isolated as covalently closed circular dnas from strains of bacillus cereus and b. subtilis. from 12 out of 15 strains of b. cereus, plasmids could be isolated. most of the b. cereus strains contained two or more plasmids. their molecular weights ranged from 1.6 x 10(6) to 105 x 10(6). bacteriocin production could be attributed to a 45 x 10(6)-dalton plasmid (pbc7) from b. cereus dsm 336, and tetracycline resistance to a 2.8 x 10(6) plasmid (pbc16) from b. cereus g ... | 1978 | 415051 |
| membrane-bound nucleotidase of bacillus cereus. | a membrane-bound nucleotidase of bacillus cereus t was solubilized by digestion with trypsin and subsequently purified more than 300-fold. the purified nucleotidase was most active on ribonucleoside 5'-monophosphates and was slightly less active (40 to 60%) on deoxyribonucleoside 5'-monophosphates and ribonucleoside 3'-monophosphates. in addition to hydrolytic activity, the nucleotidase preparation possessed phosphotransferase activity by which phosphate is transferred from a phosphate donor to ... | 1978 | 415044 |
| spore location patterns in sporulating doublets of bacillus cereus and bacillus megaterium, derived from single doublet isolates with differing sporulation geometry. | the distribution of spore loci in pairs of bacillus cereus and b. megaterium showed different degrees of polarization from random location towards location at the old end of the sporangium. when individual doublets containing both spores at either the old or new ends of the sporangia were isolated by micromanipulation, subsequent culture showed the same spore location patterns. | 1978 | 415036 |
| alteration by cereolysin of the structure of cholesterol-containing membranes. | when erythrocyte membranes were treated with cereolysin, negatively stained and examined by electron microscopy, ring and arc-shaped structures were observed in the membrane. the outside diameter of the rings varied from 33 to 50 nm with a border thickness of 6.7 to 8.3 nm. the arcs varied in length from 33 to 170 nm with a border thickness of also 6.7 to 8.3 min. when right-side-out erythrocyte ghosts which had been treated with cereolysin were examined by electron microscopy after freeze-fract ... | 1978 | 414780 |
| [clinico-epidemiologic characteristics of group illness caused by bac. cereus food poisoning]. | the authors describe an outbreak of food poisoning caused by bac. cereus embracing 80% of persons who catered at the same catering establishment. the poisoning occurred in the course of 15 hours after the moment of eating a sauce stored in warm place. in the majority of cases the course of the disease was mild; 77.8% of the patients displayed phenomena of enteritis, 20.2%--of entercolitis, and 2.0%--of gastroenterocolitis. all the patients recovered without any treatment in the course of 1--2 da ... | 1977 | 414486 |
| [admixtures of antibiotic substances in enzyme preparations of microbial origin intended for use as food additives]. | a group of enzymtic preparations, mainly of the soviet make were investigated for the presence of antibiotic substances admixtures. altogether 25 preparations obtained from molds, actinomycetes, bacteria and yeast were studied. the results showed that 18 preparations contained antibiotic substances in an amount of 0.6-1300 u, equivalent to oxytetracycline units per 1 g. most often antibiotics were present in preparations of the pectinase and protease group. in the group of glucoamylase the antib ... | 1977 | 414448 |
| the histidine residues of phospholipase c from bacillus cereus. | the inactivation of phospholipase c from bacillus cereus at ph6 by diethyl pyrocarbonate parallelled the n-ethoxyformylation of a single histidine residue in the enzyme. the inactivation arose from a decrease in the maximum velocity of the enzymic reaction with no effect on the km value. the inactivation did not apparently alter the ability of the enzyme to bind to a substrate-based affinity gel. the native enzyme contained only one reactive histidine residue. removal of the two zinc atoms from ... | 1977 | 413541 |
| lipoquinones of some bacteria and mycoplasmas, with considerations on their functional significance. | in a comparative study the lipoquinones of some chemoorganotrophic, facultatively aerobic bacteria, and representative acholeplasma, mycoplasma, spiroplasma, and thermoplasma strains were investigated. the quinones were partly purified by preparative thin layer chromatography of lipid extracts, and characterized by their difference spectra (reduced minus oxidized) and rf values. respiring bacteria expectedly contained benzoquinones and/or naphthoquinones in micromolar concentrations whereas some ... | 1977 | 413478 |
| bacillus cereus food poisoning. | 1978 | 413036 | |
| the role of various milk fractions and the importance of somatic cells in the formation of germinant(s) for bacillus cereus when milk is pasteurized. | 1977 | 412883 | |
| characterization of a bacillus cereus protease mutant defective in an early stage of spore germination. | temperature-sensitive sporulation mutants of bacillus cereus were screened for intracellular protease activity that was more heat labile than that of the parental strain. one mutant grew as well as the wild type at 30 and 37 degrees c but sporulated poorly at 37 degrees c in an enriched or minimal medium. these spores germinated very slowly in response to alanine plus adenosine or calcium dipicolinate. during germination, spores produced by the mutant rapidly became heat sensitive, but released ... | 1978 | 412836 |
| the effect of water activity and aw-controlling solute on sporulation of bacillus cereus t. | 1977 | 412827 | |
| [hygienic evaluation of insecticide preparations]. | 1977 | 412732 | |
| [the effect of sodium fluoride on the antimicrobial action of tetracycline]. | 1977 | 412595 | |
| characterization and function of intracellular proteases in sporulating bacillus cereus. | intracellular proteases from sporulating bacillus cereus have been purified by ammonium sulfate fractionation, heat treatment and deae cellulose column chromatography. after the last purification step, two protease activities, with an activity ratio of about thirty to one are resolved. both proteases are resistant to o-phenanthroline but sensitive to phenyl methyl sulfonyl fluoride. their separation by polyacrylamide gel electrophoresis and deae cellulose column chromatography, their difference ... | 1977 | 412478 |
| [phage diagnosis of strains of bacillus cereus isolated from food products]. | a total of 80 cultures of bacillus cereus, isolated from food products, were studies to establish the behaviour to specific phages or the cam set and the presence of lysogeny. a modern bacteriophage method was tested for the first time in the differentiatons of bacillus cereus, which was so far studied mainly in the differentiation of bacillus anthracis. results showed that bacteriophagy with specific type phages as a method, though not absolutely precise, is dependable for the differentiation o ... | 1977 | 412296 |
| a survey for the presence of teichuronic acid in walls of bacillus megaterium and bacillus cereus. | 1977 | 411891 | |
| relationship between the structure of carbonylcyanide phenylhydrazones and inhibition of growth of microorganisms, stimulation of respiration of yeast cells and rat liver mitochondria. | the effect of ten derivatives of carbonylcyanide phenylhydrazone on growth of bacteria, yeast and different species of filamentous fungi was investigated. in yeast and mitochondria isolated from rat liver the effect of these derivatives on the respiratory activity was also followed. the relative efficiency of the individual derivatives of carbonylcyanide phenylhydrazone was determined on the basis of the results obtained. it was shown that derivatives, in which the substituent on the benzene rin ... | 1977 | 411721 |
| [detection of cephalosporins in raw milk with the aid of b. cereus beta lactamase]. | 1977 | 411648 | |
| the fate of enterotoxigenic bacteria inoculated onto chinese barbecued foods. | 1977 | 411566 | |
| [the occurrence of bacillus cereus in dairy products (authors transl)]. | during one year 1390 samples of pasteurized milk products from five different dairies were examined for content of b. cereus. the examination was done on the day of packaging and after incubation at 17 degrees c for 24 hrs. as well. on examination on the day of packaging 8.1 % were positive. no difference could be demonstrated between low-temperature pasteurized and high-temperature pasteurized products. however, differences were found from one plant to another (2.1 %--14.5 %). after incubation ... | 1977 | 411110 |
| levels of acetyl coenzyme a, reduced and oxidized coenzyme a, and coenzyme a in disulfide linkage to protein in dormant and germinated spores and growing and sporulating cells of bacillus megaterium. | dormant spores of bacillus megaterium were found to contain approximately 850 pmol of coenzyme a (coa) per milligram of dry weight. of this total, less than 1.5% was acetyl-coa, 25% was coa-disulfide, 43% was in disulfide linkage to protein, and the remainder was the free thiol. dormand spores of bacillus cereus and clostridium bifermentans contained 700 and 600 pmol of coa per milligram of dry weight, respectively; in both species approximately 45% of the coa 45% of the coa was in disulfide lin ... | 1977 | 410791 |
| characterization of the "microprotease" from bacillus cereus. a zinc neutral endoprotease. | the neutral protease isolated from bacillus cereus (brl-70) has been purified by affinity chromatography and characterized. the enzyme exhibits a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, has a molecular weight of 34 000 by ultracentrifugation, and contains one enzymatically essential zinc atom per 34 000 g. these data together with the amino acid composition, response to metal substitution, chemical modification, and substrate specificity all indicate that this p ... | 1977 | 410441 |
| effect of some bacterial products on platelet electrophoretic mobility (pem). | bacterial lps, used in our experiments, are able to decrease pem at concentration of 100 microgram/ml after incubation with 1 x 10(7) platelets/ml for 30 min at 37 degrees c. such effect is statistically significant for bacteroides fragilis lps, s. enteritidis and s. typhi. e. coli lps 026:b6 w and e. coli 055:b5 w are not effective at all, s. minnesota (incomplete) lps 595 and 345 free of 0-polysaccharide and partially lacking core in their molecule did not influence pem, while s. minnesota lps ... | 1977 | 410424 |
| comparative studies of bacillus cereus strains isolated from various foods and food poisoning outbreaks. | 1977 | 410239 | |
| [a fatal food poisoning caused by bacillus cereus (author's transl)]. | the autopsy of a 37 year old man who had died under extraordinary circumstances showed a bromatoxism by bacillus cereus. the verification of the bacillus was possible although the man had already died 2-3 days before; and that in spite of the existence of early beginning rottenness. with the help of morphological findings the pathogenetic effect of the toxins (exotoxin and enterotoxin) of bacillus cereus is discussed. blood cultures of the heart alone are not sufficient to prove a causal connexi ... | 1977 | 410193 |
| immunocytochemical localization of spore specific antigens in ultrathin sections. | 1977 | 409707 | |
| extent of cross-linking of germ cell wall of a variant of bacillus cereus. | 1977 | 409409 | |
| antimicrobial activity of amine oxides: mode of action and structure-activity correlation. | the effect of n-alkyl derivatives of saturated heterocyclic amine oxides on the growth and metabolism of microorganisms has been studied. 4-dodecylmorpholine-n-oxide inhibited the differentiation and growth of bacillus cereus, of different species of filamentous fungi, and of the yeast saccharomyces cerevisiae. for vegetative cells, the effect of 4-dodecylmorpholine-n-oxide was lethal. cells of s. cerevisiae, after interaction with 4-dodecylmorpholine-n-oxide, released intracellular k(+) and wer ... | 1977 | 409340 |
| studies on the in vivo cellular reactions of insects: an ultrastructural analysis of nodule formation in galleria mellonella. | cellular aggregates or nodules which formed in galleria mellonella larvae in response to injections of killed bacillus cereus were examined during their first 24 hr of development. the initial aggregation stage was very rapid, occurring within 1 min, and was brought about by the release of an adhesive flocculent material by granular haemocytes in contact with the bacteria. this material, which subsequently became increasingly electron dense owing to melanin deposition, consolidated and by 5 min ... | 1977 | 408940 |
| [heterogeneity of the microcyclic development of bacillus cereus 504]. | 1977 | 408588 | |
| re-activation of guinea-pig liver uridine diphosphate glucuronosyltransferase with 1-monoacyl phosphatidylcholine after extensive phospholipid deletion at 5 degrees c with pure bacillus cereus phospholipase c. | 1977 | 408208 | |
| [regulation of isoleucine and valine biosynthesis in bacillus cereus t: possible role of threonine deaminase]. | threonine desaminase: a possible regulatory element suggested by the analysis of isoleucine, valine pathway regulation in bacillus cereus t. | 1977 | 408042 |
| studies on bacterial cell wall inhibitors. ii. inhibition of peptidoglycan synthesis in vivo and in vitro by amphomycin. | 1977 | 407940 | |
| epoxidation of aldrin to exo-dieldrin by soil bacteria. | twenty-two strains of soil bacteria, including representatives of the genera bacillus, micromonospora, mycobacterium, nocardia, streptomyces, thermoactinomyces, and pseudomonas and 10 unidentified gram-negative, motile, rod-shaped bacteria, were shown to degrade aldrin to its epoxide dieldrin. in every case, the exo-stereoisomer of dieldrin was produced exclusively. | 1977 | 407844 |
| inhibition of the synthesis of phospholipase c in bacillus cereus by a component of the growth medium. | a low molecular weight compound, probably of peptide nature, present in the beef heart extract component of the growth medium, inhibits post-transcriptionally the biosynthesis of phospholipase c by a strain of bacillus cereus. the compound also prevents the increase of proteolytic activity in the growth medium, thus suggesting that the synthesis of another enzyme, an extracellular protease, is also inhibited, and that the inhibitory compound may therefore have a more general effect on exoenzyme ... | 1977 | 407774 |
| [detection of bacillus cereus-toxins (author's transl)]. | during the logarithmic state of growth, b. cereus produced extracellular toxins, which could be precipitated by ammoniumsulphate. the toxins were not dialysable. 9 strains of b. cereus were tested and showed lethal, hemolytic, and phospholipase-c-reactions. the lethal toxin injected i.v., killed mice within 20 minutes. an intradermal injection of the 9 toxin-precipitates in rabbits produced skin reactions, which could be neutralized by a specific antiserum against the b. cereus strain b-4ac. rel ... | 1977 | 407743 |
| a case of bacillus cereus bacteraemia. | a case is presented of bacillus cereus bacteraemia in a patient receiving hepatic perfusion with 5-fluorouracil (5fu) for metastasis from a carcinoma of the breast. the literature concerning systemic b. cereus infections is briefly reviewed. | 1977 | 407561 |
| production of copper coproporphyrin iii by bacillus cereus. ii. regulation of the biosynthesis of coproporphyrin iii and its copper complex by oxygen and heavy metal ions. | the effects of oxygen and heavy metal ions on the production of copper coproporphyrin iii were studied in bacillus cereus strain 2. the formation of copper coproporphyrin iii was found to be maximum when the cells were cultivated in g-medium at a low level of oxygen supply, but it was suppressed at extremely low oxygen supply levels. when the cells were cultured in metal-free g-medium, neither metal-coproporphyrin iii nor coproporphyrin iii was formed. in the presence of copper in the medium (40 ... | 1977 | 407222 |
| production of copper coproporphyrin iii by bacillus cereus. i. purification and identification of copper coproporphyrin iii. | bacillus cereus strains 2 and t did not form spores and accumulated a large amount of purple pigment inside the cells, when cultured in a yeast extract-ammonium salt medium with excess glucose. the pigment was extracted and crystallized as the ethyl ester. it was identified as copper coproporphyrin iii. | 1977 | 407221 |
| microbiol n-acetylation of daunorubicin and daunorubicinol. | 1977 | 407204 | |
| spores of microorganisms. xxvii. some physiological and morphological characteristics of thick-walled cellular forms of bacillus cereus. | 1977 | 407136 | |
| the action of lysozyme on peptidoglycan with n-unsubstituted glucosamine residues. isolation of glycan fragments and their susceptibility to lysozyme. | 1. a peptidoglycan preparation n-acetylated at about 30% of glucosamine residues was obtained by the treatment of the lysozyme-resistant cell wall paptidoglycan of bacillus cereus with acetic anhydride at ph 7. fractionation of dialyzable material resulting from lysozyme digestion of the glycan component of this peptidoglycan preparation yielded five oligosaccharides designated as s1 to s5 besides the disaccharide glcnac-murac. 2. oligosaccharide s3, which accounted for about 30% of the disaccha ... | 1977 | 407083 |
| isolation and characterization of structural components of bacillus cereus ahu 1356 cell walls. | 1977 | 407078 | |
| studies on the disaggregation of ef-1 with carboxypeptidase a. | 1977 | 406850 | |
| microbiological transformations of delta6a10a-tetrahydrocannabinol. | a screening program was conducted to find microorganisms that catalyze transformation reactions with cannabinoids. three hundred fifty-eight cultures, consisting of 97 bacteria, 175 actinomycetes, and 86 molds, were incubated in media containing 0.5 mg of delta(6a,10a)-tetrahydrocannabinol (delta(6a,10a)-thc) per ml. after 120 h of cultivation, ethyl acetate extracts of the cultures were examined by thin-layer chromatography (tlc) for transformation products. about 18% of the cultures modified d ... | 1977 | 406841 |
| study of inhibition of outgrowth in bacillus cereus t by ethyl picolinate. | the effects of ethyl picolinate on germination, outgrowth, and sporulation of bacillus cereus t were studied in a synthetic medium containing glucose. ethyl picolinate specifically inhibited at two stages, outgrowth and sporulation. the initiation of germination and cell division was not affected. the inhibition of outgrowth by ethyl picolinate could be reversed by enrichment of inoculum with aspartic acid, asparagine, lysine, phenylalanine, and tyrosine among the amino acids and by oxalacetate. ... | 1977 | 406840 |
| a phosphatidylinositol phospholipase c (phosphatasemia factor)of bacillus cereus. | 1977 | 406439 | |
| the effect of storage on drugs incorporated into pelleted fish food. | 1977 | 406257 | |
| severe systemic and pyogenic infections with bacillus cereus. | 1977 | 406000 | |
| a severe necrotic enterotoxin produced by certain food, food poisoning and other clinical isolates of bacillus cereus. | the ability of certain strains of bacillus cereus consistently to elaborate a filterable non-dialysable toxin capable of causing severe disruption and necrosis of the intestinal mucosa and submucosa is confirmed. this property is not universal to all b. cereus strains and different degrees of production of this toxin are exhibited by the different strains which produce it. the necrotic effect is produced by whole-cell cultures of the toxin producing strains in broth and in boiled rice. some char ... | 1977 | 405982 |
| fate of foreign dna taken up by tonsillar lymphocytes. | the uptake of heterologous (b. cereus) dna by tonsillar lymphocytes was followed for 22 hours. acid-precipitable dna became associated with the cells as soon as they were mixed with dna. the uptake was linear from 0 to 3 hours, between 3 to 6 hours part of the cell-associated dna was released into the incubation medium, and at 22 hours the amount of label in the cells reached the values measured at 2--3 hours. in respect of the size of dna the uptake seemed to follow the same saturation curve. t ... | 1977 | 405837 |
| an investigation of the bacteriological quality of retail vanilla slices. | one hundred and thiry-three vanilla slices, purchased from shops in the west yorkshire metropolitan county, were examined to determine the numbers and types of bacteria present at the time of purchase. the surface colony count at 37 degrees c was greater than 10(3)/g in 67/133 (50%) of the samples examined, bacillus cereus being found at that concentration in 21.8%, coliform bacilli including e. coli in 5.3%, staphylococcus aureus in 3-0% and streptococcus faecalis in 0-8%. thirty-four strains o ... | 1977 | 405420 |
| microbial production of vitamin b12 antimetabolites. iii compound 102804 from bacillus cereus. | a basic compound with empirical formula c12h16n2o5 was isolated from bacillus cereus 102804 fermentations of a soybean meal-glucose medium. the inhibitory activity of compound 102804 on growth of gram-positive and gram-negative bacteria growing in a chemically defined medium was reversed by vitamin b12, by l-methionine, and by d-methionine. it has no inhibitory activity for escherichia coli (davis 113-3) when grown in media containing l-methionine. the biosynthesis of 102804 coincides with the s ... | 1977 | 405359 |
| the inactivation of beta-lactamase i from b. cereus by dicloxacillin: effect of buffer composition on the kinetic characteristics of the modified enzyme. | buffer composition affects the pattern of inactivation of b. cereus beta-lactamase i by dicloxacillin. at ph 7.0, in the presence of phosphate ions, dicloxacillin appears to cause essentially an irreversible inactivation of the catalytic site of the enzyme, while in a tris-cacodylate buffer the inactivation process appears to affect essentially the substrate-binding site. buffer ions act presumably by interacting themselves with either the catalytic or the substrate-binding site of the enzyme, t ... | 1977 | 405348 |
| inactivation of phospholipase c from bacillus cereus by a carboxyl group modifying reagent. | phospholipase c from bacillus cereus was inactivated by incubation with either of the carboxyl reagents, a water-soluble carbodimide plus a nucleophile or woodward's reagent k. with the former reagent, the incorporation into the enzyme of the first mol of nucleophile caused a 4-5-fold increase in the km for dihexanoyllecithin with no significant effect on the vm. the second mol of nucleophile incorporated caused no further change in km but destroyed most of the catalytic activity. modification o ... | 1977 | 404815 |
| precipitating antibody against protease of corynebacterium pyogenes in pigs. | antibody in sera from pigs carrying an abscess associated with corynebacterium pyogenes and healthy pigs was examined by the agar gel diffusion test. in the test, the concentrated culture fluid containing the protease of c. pyogenes was used as antigen. as a result, precipitating antibody was demonstrated in sera from 25 of 30 abscessed pigs and a few of the healthy pigs. when the relationship between precipitating antibody and protease was examined by the immunoelectrophoresis and gel filtratio ... | 1977 | 404574 |
| role of glutamate in the sporogenesis of bacillus cereus. | bacillus cereus t, sporulating in a chemically defined medium under optimum conditions, requires substrate quantities of glutamate during the first 4 h of sporogenesis. seventy percent of the glutamate utilized was catabolized to co2 during this period, with the remaining glutamate carbon assimilated into various spore constituents, principally protein and nucleic acid. the importance of glutamate as the primary source of reducing potential and energy for early stages of spore formation was inve ... | 1977 | 404283 |
| enzymic assays for isomers of 2,6-diaminopimelic acid in walls of bacillus cereus and bacillus megaterium. | an enzymic assay for individual isomers (meso-, ll- and dd-) of 2,6-diaminopimelate was developed. the enzyme 2,6-diaminopimelate decarboxylase specifically attacked meso-diaminopimelate and was used to measure this isomer manometrically. the meso- and ll-isomers were measured together manometrically in a coupled assay with diaminopimelate decarboxylase and diaminopimelate epimerase (which converts ll-diaminopimelate into meso-diaminopimelate). the dd-isomer was not attacked by either enzyme and ... | 1977 | 403912 |
| phospholipase c from bacillus cereus. evidence for essential lysine residues. | 1. phospholipase c was inactivated by exposure to the three amino-group reagents, ethyl acetamidate, 2,4,6-trinitrobenzensulphonic acid and pyridoxal 5'-phosphate plus reduction. 2. inactivation by pyridoxal 5'-phosphate showed the characteristics of schiff's base formation with the enzyme. the pyridoxal 5'-phosphate-treated enzyme after reduction had an absorbance maximum at 325 mm and 6-n-pyridoxyl-lysine was the only fluorescent component after acid hydrolysis. 3. for complete inactivation, 2 ... | 1977 | 403907 |
| initiation of bacillus spore germination by hydrostatic pressure: effect of temperature. | suspensions of bacillus cereus t, b. subtilis, and b. pumilus spores in water or potassium phosphate buffer were germinated by hydrostatic pressures of between 325 and 975 atm. kinetics of germination at temperatures within the range of 25 to 44 degrees c were determined, and thermodynamic parameters were calculated. the optimum temperature for germination was dependent on pressure, species, suspending medium, and storage time after heat activation. germination rates increased significantly with ... | 1977 | 403174 |
| rapid fluorimetric assay of minocycline in plasma or serum: comparison with microbiological assay. | 1. plasma concentrations of minocycline were assayed by measuring the fluorescence emission of an aluminum-minocycline chelate in a spectrofluorimeter. 2. the assay gives reproducible results over the concentration range 0.05-5.0 mug ml-1, and compared favourably with results achieved using a conventional large plate agar diffusion microbiological assay in a double-blind comparison of the two methods. 3. fluorimetric assay of minocycline in plasma was found to correlate closely with antibiotic a ... | 1977 | 402926 |
| biochemical properties of clostridium bifermentans spores. | as previously found for spores of bacillus species, dormant spores of clostridium bifermentans contained essentially no adenosine triphosphate, a high level of adenosine monophosphate, a high level of 3-phosphoglyceric acid, and much transfer ribonucleic acid lacking a 3'-terminal adenosine monophosphate residue. as in spores of bacillus species, germination of c. bifermentans spores was accompanied by utilization of the 3-phosphoglyceric acid, a large increase in the adenosine triphosphate leve ... | 1977 | 402349 |
| bacillus cereus pneumonia. survival in a patient with cavitary disease treated with gentamicin. | cavitary pneumonitis due to bacillus cereus occurred in a patient with acute lymphoblastic leukemia. pneumonia due to this organism is uncommon and recovery has not been previously reported. the present report is the first description of cavitary pneumonia due to b. cereus with a successful outcome. recovery was attributed to appropriate antimicrobial therapy and induction of leukemic remission with chemotherapy. | 1977 | 402096 |
| serotypes of bacillus cereus from outbreaks of food poisoning and from routine foods. | a provisional serotyping scheme was used to type cultures of bacillus cereus from 84 outbreaks of food poisoning in seven countries; 283 of the 337 (84%) cultures tested were typable. in 35 of the 61 outbreaks associated with a vomiting-type syndrome, foods, clinical specimens or both yielded h-serotype 1 only. type 1 strains together with other sterotypes were isolated in seven outbreaks. in 14 outbreaks types 3, 4, 5, 8 or a mixture of serotypes were present. untypable strains were isolated in ... | 1977 | 401846 |
| the variable response of bacteria to excess ferric iron in host tissues. | the enhancement by exogenous ferric iron, both systemic and local, of the infectivity of 120 strains of bacteria, representing 17 genera, was measured in the skin of guinea-pigs. systemic iron enhanced only 23% of 115 strains, and local iron 49% of 71 strains. systemic iron, by an apparently anti-inflammatory action, depressed the size of lesions produced by 27 of the non-enhanced strains from nine of the genera tested. for most strains, the degree of enhancement was small, ranging from 2- to 8- ... | 1979 | 372534 |
| septicemia in children with cancer. | because of the persistently high mortality from sepsis in cancer patients, a retrospective study was designed to identify the causative organisms and to determine the factors affecting the outcome of sepsis. a total of 84 episodes of septicemia in 61 children with cancer were studied. the more frequently isolated organisms were: staphylococcus aureus (21.4%); escherichia coli (18%); klebsiella (7.1%); pseudomonas (6%); and bacteroides fragilis (6%). other isolates included proteus, serratia, aci ... | 1979 | 371000 |
| membrane ultrastructural changes during calcium phosphate-induced fusion of human erythrocyte ghosts. | nascent calcium phosphate promotes the agglutination and fusion of human erythrocyte ghosts. membrane phospholipids of erythrocyte ghosts treated with ca2+ and phosphate ions become exposed to attack by phospholipase c (phosphatidylcholine cholinephosphohydrolase, ec 3.1.4.3) (bacillus cereus). freeze-fracture pictures of fused erythrocyte ghosts show the presence of regions deficient in intramemebrane particles in the protoplasmic face which we believe to be regions of fusion. discontinuous reg ... | 1977 | 329283 |
| identification of histidine residues that act as zinc ligands in beta-lactamase ii by differential tritium exchange. | 1. four histidine-containing peptides have been isolated from a tryptic digest of the zn2+-requiring beta-lactamase ii from bacillus cereus. one of these peptides probably contains two histidine residues. 2. the presence of one equivalent of zn2+ substantially decreases the rate of exchange of the c-2 proton in at least two and probably three of the histidine residues of these peptides for solvent 3h. 3. it is concluded that peptides containing at least two of the three histidine residues acting ... | 1979 | 314287 |
| predicted secondary structures of four penicillin beta-lactamases and a comparison with two lysozymes. | we have predicted the secondary structures of four beta-lactamases (bacillus cereus, bacillus licheniformis, staphylococcus aureus, and escherichia coli r-tem) by the statistical method of chou & fasman as well as by the information theory method of garnier et al. the secondary structures of all four beta-lactamases are of the alpha/beta type (levitt & chothia's nomenclature), with helices at n- and c-termini. there are about eight short regions each of alpha-helical (30--50%) and beta-strand (1 ... | 1979 | 313376 |
| cardiolipin: a stereospecifically spin-labeled analogue and its specific enzymic hydrolysis. | the spin-labeled cardiolipin 1-(3-sn-phosphatidyl)-3-[1-acyl-2-(16-doxylstearoyl)glycero(3)phosphol]-sn-glycerol has been prepared. the stereoselective synthesis makes use of the monolysocardiolipin 1-(3-sn-phosphatidyl)-3-[1-acyl-2-lyso-sn-glycero(3)phospho]-sn-glycerol, available from the stereospecific hydrolysis of cardiolipin by phospholipase a2 (phosphatide 2-acylhydrolase, ec 3.1.1.4) of trimeresurus flavoviridis. the results of treatment of the spin-labeled cardiolipin with the cardiolip ... | 1978 | 274715 |
| production and property of beta-lactamases in streptomyces. | the production of beta-lactamases by 100 strains of streptomyces was studied. about one-half of the strains produced more than 2.3 u of beta-lactamase per ml, and another half produced less than 1.4 u/ml. the amounts of beta-lactamases produced by two strains were in the order of those produced by bacillus cereus 569/h and bacillus licheniformis 749/c. these streptomyces enzymes function primarily as penicillinases rather than cephalosporinases. properties such as ph optimum, substrate specifici ... | 1975 | 242252 |
| [report of "lethal factor" synthetized by bacillus cereus (author's transl)]. | bacillus cereus growing in nutrition broth produces during logarithmic growth and in stationary phase a lethal factor extremely toxic for rodents. this toxin is only produced in the presence of oxygen and depends on bacterial replication. the highest titers of toxin are obtained using bacteria in a concentration of 10(7) to 10(9) per ml of nutrition broth. during regression phase of bacillus cereus the titer of toxin declines to zero. toxicity of the lethal factor becomes not altered in an alcal ... | 1975 | 242167 |
| biological characteristics of an enterotoxin produced by bacillus cereus. | an enterotoxin synthesized during exponential growth by bacillus cereus produces fluid accumulation in rabbit ileal loops, alters vascular permeability in the skin of rabbits, and kills mice when injected intravenously. all activities are eluted simultaneously from a sephadex g-75 column and are distinct from the hemolysin and egg yolk turbidity factor of b. cereus. the enterotoxin is a true exotoxin. it interacts with intestinal receptor sites in a highly transient manner in the ileal loop syst ... | 1975 | 240499 |
| [formation by bacteria of the genus bacillus of coagulases that coagulate plasma]. | 1975 | 240446 | |
| effect of ph on sporulation of bacillus stearothermophilus. | an improved broth medium was developed for high growth yields of bacillus subtilis var. niger ncib 8649, bacillus cereus ncib 9373, and bacillus stearothermophilus ncib 8919 and atcc 7953. sporulation was abundant (1.1 times 10-8 b. subtilis var. niger and 9.2 times 10-7 b. cereus per ml) at an initial ph of 7.0. sporulation of both strains of b. stearothermophilus took place (1.9 times 10-7 and 2.4 times 10-7/ml, respectively) in this medium when initial ph values of 7.7 to 8.7 were used. | 1975 | 238470 |
| inositol cyclis phosphate as a product of phosphatidylinositol breakdown by phospholipase c (bacillus cereus). | 1975 | 236918 | |
| the influence of growth and sporulation temperature on heat resistance of a bacterial nucleosidase. | 1975 | 236744 | |
| isolation of two new related peptide antibiotics, cerexins a and b (studies on antibiotics from the genus bacillus. i). | two new antibiotics cerexins a and b were isolated from different strains identified with bacillus cereus. these two antibiotics are amphoteric in nature, soluble in particular solvents such as dimethylsulfoxide, dimethylformamide and alkaline water, and show typical infrared absorptions of peptide. these also have similar antimicrobial properties active against gram-postive bacteria. | 1975 | 236271 |
| analysis of bacterial biotin-proteins. | the biotin-protein populations in several bacterial strains were analyzed by solubilization of [3h]biotin-labeled cells with sodium dodecylsulfate followed by electrophoresis on polyacrylamide gels containing the detergent. a variety of patterns of biotin-labeled polypeptide chains was seen, ranging from a single biotin-protein in escherichia coli, corresponding to the biotin carboxyl carrier protein component of acetyl-coa carboxylase, to multiple species in enterobacter aerogenes, pseudomonas ... | 1975 | 235315 |
| effect of ph and sodium chloride on growth of bacillus cereus in laboratory media and certain foods. | the effects of nacl concentration, ph, and water activity (aw) on the ability of vegetative cells of bacillus cereus to initiate aerobic growth in brain heart infusion broth at 30 c were studied in a factorial design experiment. by using multiple regression techniques, equations were derived which related the decimal reduction of the bacterial population to the concentration of nacl and ph of broth to which the population was exposed. from these equations, the percentage of inoculated cells capa ... | 1975 | 234158 |
| synthesis of choline and ethanolamine phospholipids with thiophosphoester bonds as substrates for phospholipase c. | spectrophotometric assays of esterases are sensitive, rapid, and quite specific when thioester substrates are used. glycerophospholipids with thiophosphoester bonds may be useful as substrates for phospholipase c (ec 3.1.4.3). these have been made from mercaptoglycerol and mercaptoethanol. the thiols were oxidized to disulfides, acylated, and reduced with dithiothreitol. phosphocholine derivatives were made by the classical methods for oxyphosphoesters. the phosphatidyl choline analogue was conv ... | 1979 | 231487 |
| metal-coordinating substrate analogs as inhibitors of metalloenzymes. | a group of active-site metal coordinating inhibitors of zinc proteases (carboxypeptidase a, thermolysin, bacillus cereus neutral protease, and angiotensin-converting enzyme) have been synthesized and their properties investigated. their general structures are r-sh and r-nh-po2(o phi)h, where-s- or -o- serve as metal ligands and r refers to an amino acid or peptide group designed to interact with substrate recognition sites. these inhibitors can be extremely potent; thus, n-(2-mercaptoacetyl)-d-p ... | 1979 | 230502 |
| the role of manganese in growth and sporulation of bacillus subtilis. | phosphoglycerate phosphomutase of bacillus subtilis, bacillus cereus and bacillus megaterium required mn2+ as cofactor, whereas the wheat germ and rabbit liver enzymes did not. in the absence of mn2+, b. subtilis did not sporulate in normal sporulation media but it did sporulate if the proper ratio of glucose or glycerol and malate was used. decoyinine, an inhibitor of guanosine monophosphate synthesis, induced sporulation in the presence of excess glucose and malate to the same extent with and ... | 1979 | 225409 |
| [electron microscopic study of normal bacillus anthracoides spores and after exposure to a chloroactive disinfectant]. | the fine structure of bacillus anthracoides spores is similar in general to that of other, taxonomically related species of spore forming bacteria. however, the former is characterized by a well-developed multilayered exosporium and the heterogeneous structure of an envelope. the lethal effect of a chloroactive disinfectant (2/3 of calcium hypochlorite basic salt) is caused by changes in the structural organization of spores, which interferes with the normal permeability barrier and metabolic pr ... | 1979 | 224288 |
| preparation and properties of an immobilized derivative of penicillinase. | penicillinase (beta-lactamase i, ec 3.5.2.6) secreted by bacillus cereus, strain 569/h, was covalently attached to aminoethyl cellulose via glutaraldehyde. the immobilized derivative shows increased thermostability and decreased susceptibility to conformational changes induced by certain substrates of penicillinase. the decline in the rate of hydrolysis of such substrates was consequently suppressed by immobilization. a marked increase in km was observed with all substrates except for the unsubs ... | 1979 | 219920 |
| phosphatase activity of aerobic and facultative anaerobic bacteria. | 1115 strains of aerobic and facultatively anaerobic bacteria were tested for phosphatase activity by a conventional plate method and a microtest. the microtest was devised to allow results to be read after 4 h cultivation. phosphatase activity was found in wide range of species and strains. besides staphylococci, where the test for phosphatase is successfully used, it may be applied as one of the valuable tests for the differentiation of the following species: bacillus cereus, b. licheniformis, ... | 1978 | 216188 |
| the use of phospholipase c to detect structural changes in the membranes of human erythrocytes aged by storage. | 1978 | 213113 | |
| foodborne hazards of microbial origin. | foods can serve as vehicles of many pathogenic and toxigenic agents of disease. bacterial agents comprise three groups: 1) those that grow in the food and produce an active toxin before consumption (e.g., clostridium botulinium); 2) those that merely exist as contaminants in the food but are able to initiate infection when swallowed (e.g., salmonella spp.); and 3) those that multiply and produce large numbers of vegetative cells in the food, then release an active enterotoxin when they sporulate ... | 1978 | 212326 |
| hydrolytic action of phospholipases on sealed ghosts of mammalian erythrocytes. | 1978 | 211122 | |
| [enzymatic mechanisms for antimicrobial protection of the oral cavity]. | data on the role of oral lysozyme, ribonuclease, deoxyribonuclease and peroxidase in antimicrobial defense of the macroorganism are reviewed. the biochemical and physiological properties of the enzymes secreted by salivary glands and released from emigrating leukocytes are discussed. spectra of antimicrobial action of the enzymes and participation of these enzymes in maintaining the stability of oral biocenosis are described as well as the regulation of these enzymatic activities and the pathoge ... | 1978 | 208288 |
| synthesis and quantitative structure-activity relationships of antibacterial 1-(substituted benzhydryl)-4-(5-nitro-2-furfurylideneamino) piperazines. | 1-benzhydryl -4- (5-nitro-2-furfurylideneamino) piperazine and 11 substituted analogs were prepared and examined for in vitro antimicrobial activity. the compounds were active against bacillus cereus 7, bacillus megaterium 122, bacillus subtilis 104, clostridium perfringens 13, and the tetracycline-resistant clostridium perfringens 37. regression analyses on the antibacterial activity data based on the hansch approach, using pi, pi2, and sigma parameters, yielded several statistically significan ... | 1978 | 207855 |