Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| pathophysiologic features of swine dysentery: cyclic nucleotide-independent production of diarrhea. | net electrolyte and water transport and unidirectional na+ fluxes were examined in ligated colonic loops of clinically normal pigs and in pigs with swine dysentery (etiologic agent treponema hyodysenteriae) in the presence or absence of theophylline. in normal pigs, theophylline abolished net na+ absorption via a reduction in the lumen-to-blood flux, decreased cl- absorption, and increased hco3- accumulation in the lumen. in infected pigs, all net ion transport was abolished, with the addition o ... | 1983 | 6309041 |
| the expression of biologically active cholera toxin in escherichia coli. | chromosomal dna from vibrio cholerae e1 tor strain 1621 was digested with hind iii and the fragments obtained fractionated by centrifugation through a sucrose gradient. a 15kb fragment which contained the toxin gene of v. cholerae was identified by its homology with the heat labile toxin (lt) gene of toxigenic e. coli. this fragment was cloned in e. coli using pat153 and subsequently characterized by restriction endonuclease digestion. sequences homologous to the lt gene were identified by hybri ... | 1983 | 6307786 |
| [sporadic case or the onset of a new cholera epidemic]. | 1983 | 6307536 | |
| value of stool examination in patients with diarrhoea. | findings of stool examinations in 1593 patients with diarrhoea due to a single enteric pathogen--enterotoxigenic escherichia coli rotavirus, shigella, campylobacter jejuni, vibrio cholerae 0:1, entamoeba histolytica, or giardia lamblia--were reviewed to determine how well they predicted the agent associated with the diarrhoea. specimens were examined visually for blood and mucus, tested for ph, and examined under a microscope for the presence of red and white blood cells, parasites, and stool fa ... | 1983 | 6307460 |
| aetiological studies on hospital in-patients with acute diarrhoea in calcutta. | bacterial enteropathogens and rotavirus were sought in 356 cases with acute diarrhoea admitted to the infectious diseases hospital, calcutta. one or more pathogens were isolated from 74.7% of the cases. single enteropathogens could be detected from 66% and multiple enteropathogens from 8.7% of the patients. vibrio cholerae biotype el tor, rotavirus, v. parahaemolyticus, and enteropathogenic and enterotoxigenic escherichia coli were the major pathogens detected. rotavirus was detected from 7.6% o ... | 1983 | 6306873 |
| action of alpha-galactosidase from clostridium sporogenes and coffee beans on blood group b antigen of erythrocytes. the effect on the viability of erythrocytes in circulation. | the effect of alpha-galactosidase, purified from clostridium sporogenes (maebashi), was examined on erythrocytes from rats, rabbits and gibbons. the amount of galactose released by alpha-galactosidase from cl. sporogenes and from coffee beans was compared. the amount of sialic acid released by vibrio cholera sialidase was also determined. loss of blood group b specificity following treatment with alpha-galactosidase was demonstrated with anti-b lectin. in animal models, removal of all the alpha- ... | 1983 | 6304953 |
| the specificity of viral and bacterial sialidases for alpha(2-3)- and alpha(2-6)-linked sialic acids in glycoproteins. | the anomeric specificity of six sialidases (vibrio cholerae, arthrobacter ureafaciens, clostridium perfringens, newcastle disease virus, fowl plague virus and influenza a2 virus sialidases) was assessed with sialylated antifreeze glycoprotein, ovine submandibular gland glycoprotein and alpha 1-acid glycoprotein, resialylated specifically in alpha(2-3) or alpha(2-6) linkage with n-acetylneuraminic acid or n-glycolylneuraminic acid using highly purified sialyltransferases. the rate of release of s ... | 1983 | 6301560 |
| direct assay of bound sialic acids on rat spermatozoa from the caput and cauda epididymidis. | bound sialic acids on rat spermatozoa were assayed by oxidation with 1 mm-naio4 at 0 degree c, liberating c-9 as formaldehyde which was further quantitated using 3-methyl-2-benzothiazolinone. the mean +/- s.d. (n = 20) content of bound sialic acids of spermatozoa from the caput and cauda epididymidis was 50.9 +/- 8.0 and 25.2 +/- 3.8 nmol/10(8) spermatozoa respectively. about 85% of the former and 75% of the latter could be extracted by 1% triton x-100 and 2 mm-dithiothreitol. about 70% of the f ... | 1983 | 6300382 |
| automatic and manual latex agglutination tests for measurement of cholera toxin and heat-labile enterotoxin of escherichia coli. | automated and manual latex agglutination methods were employed to measure cholera toxin (ct), heat-labile enterotoxin (lt) of escherichia coli, and their subunits a and b. dow polystyrene latex particles (diameter, 0.22 microns) and polystyrene-chlorostyrene latex particles (diameter, 1 micron) were sensitized by rabbit-specific immunoglobulin for each antigen and used as the reagents of the automated and manual agglutination tests, respectively. automated agglutination was performed by a nephel ... | 1983 | 6298275 |
| receptor-like glycocompounds in human milk that inhibit classical and el tor vibrio cholerae cell adherence (hemagglutination). | the two biotypes of vibrio cholerae were found to have cell-associated hemagglutinins which differ with regard to binding to different species of erythrocytes and inhibition by monosaccharides. a total of 12 classical v. cholerae strains (inaba or ogawa) strongly agglutinated human erythrocytes in a reaction specifically inhibited by l-fucose, whereas 12 el tor strains preferably agglutinated chicken erythrocytes, a reaction reversed by d-mannose or by higher concentrations of d-fructose, d-gluc ... | 1983 | 6295953 |
| effects of temperature and salinity on vibrio cholerae growth. | laboratory microecosystems (microcosms) prepared with a chemically defined sea salt solution were used to study effects of selected environmental parameters on growth and activity of vibrio cholerae. growth responses under simulated estuarine conditions of 10 strains of v. cholerae, including clinical and environmental isolates as well as serovars o1 and non-o1, were compared, and all strains yielded populations of approximately the same final size. effects of salinity and temperature on extende ... | 1982 | 6295276 |
| experimental studies on enteropathogenicity of vibrio cholerae serotypes other than 1. | vibrio cholerae serotypes other than i have come to be recognised as an important aetiological agent of choleraic diarrhoea and a few of them have been shown to ben enterotoxigenic in experimental models. this study was taken up with strains belonging to all the known 59 serotypes. live cells and culture filtrates of all the strains could cause accumulation of fluid in rabbit ileal loops. however, eleven strains did so after 1-2 serial passages in rabbit gut. all the culture filtrates could also ... | 1982 | 6295024 |
| [vibrio eltor mutants with altered hemolysin production: their isolation and characteristics]. | hemolysin hyper- hypoproductive vibrio eltor mutants and hemolytically inactive clones were isolated using tetracycline (tn10) and chloramphenicol (tn9) resistance transposons. chromosomal localization was demonstrated for hml gene(s) involved in hemolysin production. mutations responsible for an altered hemolysin production are likely to be situated closely to pur ura arg met chromosomal markers. | 1982 | 6293923 |
| [primary mapping of the oag locus determining the synthesis of the somatic o-antigen on the vibrio eltor chromosome]. | 1982 | 6293917 | |
| characteristics of boric acid tolerant vibrio cholerae. | two strains of boric acid tolerant vibrio cholerae have been studied in order to identify the characteristics which distinguish them from the 'wild-type' virulent strains. strain ib5 requires either amino acid cysteine or methionine while strain ib5s requires deoxyribonucleic acid (dna) for growth. although the strains did not grow on desoxycholate citrate agar (dca) they grew well on chocolate agar. morphologically filamentous, bacillary and comma-shaped forms were seen, depending on the media ... | 1982 | 6291353 |
| the expression of biologically active cholera toxin in escherichia coli. | chromosomal dna from vibrio cholerae el tor strain 1621 was digested with hind iii and the products fractionated by centrifugation through a sucrose gradient. a 15kb fragment containing the toxin gene of v. cholerae was identified by its homology with the heat labile toxin (lt) gene of toxigenic e. coli. this fragment was cloned in e. coli using pat153 and subsequently characterised by digestion with different restriction endonucleases. sequences homologous to the lt gene were identified by hybr ... | 1982 | 6290992 |
| purification of chlamydia trachomatis lymphogranuloma venereum elementary bodies and their interaction with hela cells. | a procedure has been developed to yield infectious elementary bodies of the lymphogranuloma venereum strains lgv 434 and 404 of chlamydia trachomatis, labelled during intracellular growth in hela 229 cells. the final preparation, obtained after velocity sedimentation of a polycarbonate membrane-filtered sample through a sucrose gradient, is free of host proteins and, more importantly, of chlamydial reticulate bodies. using such purified preparations, it was found that the association of lgv 434 ... | 1982 | 6288839 |
| [isolation of vibrio eltor mutants producing melanin and the mapping of the mel locus using transposons]. | melanin-producing v. eltor mutants obtained by means of the transposon which determines resistance to tetracycline (tn 10) are described. gene mel is believed to be localized on the chromosome of v. eltor in the region of markers his trp met ura rif arg ilv. | 1982 | 6287769 |
| isolation and partial characterization of surface components of cell line mda-mb-231 derived from a human metastatic breast carcinoma. | neuraminidase (vibrio cholerae) treatment of human metastatic mammary carcinoma mda-mb-231 cells grown in culture released 0.60-0.63 mg of n-acetylneuraminic acid from 10(9) cells. incubation of intact cells with a modified trypsin and fractionation by gel filtration gave mainly o-glycopeptides. the presence of o-glycosyl-linked chains having one or two carbohydrate residues was confirmed by treatment of the glycopeptide fractions with galactose oxidase, followed by reduction with alkaline sodiu ... | 1982 | 6287076 |
| comparison of paired whole milk and dried filter paper samples for anti-enterotoxin and anti-rotavirus activities. | milk specimens, 75 from cows immunized against cholera toxin and 35 from a human population in which enterotoxigenic escherichia coli and rotaviral infections are endemic, were collected as paired filter paper and frozen whole milk samples. each pair was tested for antibody activity against heat-labile e. coli and vibrio cholerae enterotoxins. additionally, 12 of the 35 paired human milk samples stored as frozen whole milk and dried on filter paper were tested for anti-rotavirus immunoglobulin a ... | 1982 | 6286715 |
| longitudinal studies of infectious diseases and physical growth of children in rural bangladesh. ii. incidence of diarrhea and association with known pathogens. | longitudinal studies were done in two villages rural bangladesh to learn more about the interactions between infectious diseases and the nutritional status of children. diarrheal diseases, identified by surveillance of 197 children aged 2-60 months, were studied for bacterial, viral and parasitic enteropathogens in 1978-1979. the annual incidence of diarrhea was highest in children aged 2-11 months, and declined progressively with age from seven to four episodes per child per year. an enteropath ... | 1982 | 6278925 |
| potentiating effect of bile on enterotoxin-induced diarrhea. | the influence of bile acids on adenosine 3',5'-phosphate-induced intestinal secretion was studied in mice. bile flow was stopped by ligation of the common bile duct, and secretion was induced in ligated loops of the small intestine. the decrease of bile led to inhibition of hypersecretion after challenge with heat-labile enterotoxins from vibrio cholerae and escherichia coli, as well as with prostaglandin e1. in contrast, the fluid response induced by dibutyryl-adenosine 3',5'-phosphate was unaf ... | 1982 | 6276300 |
| viruses and bacteria in pediatric diarrhea in thailand: a study of multiple antibiotic-resistant enteric pathogens. | children with diarrhea admitted to a rehydration ward of a children's hospital in bangkok were investigated to determine the prevalence of enteric pathogens, the extent of children's previous antibiotic therapy, and the frequency of plasmid-mediated antibiotic resistance among infecting bacteria. rotavirus (36%), enterotoxigenic escherichia coli (18%), shigella (9%), salmonella (6%), campylobacter jejuni/coli (4%), and vibrio cholerae (2%) infections were among 105 children with diarrhea. antibi ... | 1981 | 6275727 |
| [1h-nmr spectroscopy--a potent method for the determination of substrate specificity of sialidases (author's transl)]. | we describe here the application of 1h-nmr spectroscopy to determine the substrate specificity of sialidases using a 1:1 mixture of neuac alpha 2-3gal beta 1-4glc and neuac alpha 2-6gal beta 1-4glc, one viral and five bacterial sialidases. this method utilizes the separate signals in nmr spectra, characteristic for the different alpha ketosidically linked neuac residues and also for bound and free neuac. the signals generally most suitable for these purposes are those of h3a, h3e and ncoch3. by ... | 1981 | 6273284 |
| specific gangliosides function as host cell receptors for sendai virus. | the ability of specific gangliosides to function as host cell receptors for sendai virus was investigated by using madin-darby bovine kidney cells which become resistant to infection upon treatment with vibrio cholerae sialidase. sialidase-treated cells were incubated for 20 min at 37 degrees c with individual, highly purified gangliosides containing homogeneous carbohydrate moieties and then inoculated with virus for 10 min. susceptibility of the cells to infection was monitored by hemagglutina ... | 1981 | 6272300 |
| comparison of extracellular & intracellular level of cyclic amp between the toxinogenic & non-toxinogenic strains of vibrio cholerae. | 1981 | 6269992 | |
| transposon-facilitated recombination in classical biotypes of vibrio cholerae. | transposon-facilitated recombination (tfr) donors of classical vibrio cholerae strain 162 were constructed by introducing the ampicillin transposon tn1 into the p conjugative plasmid and the bacterial chromosome. the improved donors mediated high-frequency, polarized transfer of chromosomal genes from origins to confirm the gene orders of the previous classical strain 162 genetic map and to establish its circularity. significant transfer of linked genes from e1 tor tfr donors to classical recipi ... | 1981 | 6265372 |
| [methods of biologic testing of enterotoxins]. | 1981 | 6264715 | |
| [role of cyclic amp in bacterial physiology. advances of research since 1973]. | 1980 | 6264180 | |
| the determination and localization of sialic acid in guinea-pig granulocytes. | when intact guinea-pig granulocytes (polymorphonuclear leucocytes) disrupted by sonication or with detergent were treated with neuraminidase from vibrio cholerae, 3.1--3.2 nmol of sialic acid/10(7) cells was released. by using a chromatographic procedure for the specific determination of total cell sialic acid, this releasable portion was found to constitute 70% of the total sialate. all of the neuraminidase-releasable sialic acid of the cells could be removed by enzymic treatment of intact cell ... | 1980 | 6263258 |
| specific monosaccharide inhibition of active sodium channels in neuroblastoma cells. | l-fucose and d-galactose in low concentrations (0.27 or 2.7 mm) inhibited the induction of active na+ channels in mouse and human neuroblastoma cells when the monosaccharides were added to the culture medium for 4 days with the inducing agent dimethyl sulfoxide. active na+ ionophores were determined by measurement of the toxin-stimulated efflux of 86rb from the cells. at the same time, the amount of a radioactive glycoprotein (mr 200,000), which was shown previously to be associated with neurite ... | 1981 | 6262760 |
| stool electrolyte content and purging rates in diarrhea caused by rotavirus, enterotoxigenic e. coli, and v. cholerae in children. | one hundred twenty children below 5 years of age with diarrhea caused by vibrio cholerae, enterotoxigenic escherichia coli, or rotavirus were studied for stool electrolyte composition and purging rates. the mean purging rate in cholera was 60.1 ml, in etec 39.2 ml, and in rotavirus infection 31.4 ml/kg/8 hour. the mean stool sodium concentration in cholera was 88.9 mmol/l, in etec 53.7 mmol/l, and in rotavirus infection 37.2 mmol/l. stool potassium concentration did not show much variation, mean ... | 1981 | 6262471 |
| vibrio cholerae conjugative plasmid psj15 contains transposable prophage dvca1. | evidence is presented that defective prophage dvca1 in vibrio cholerae strain 162 was transposed to the hybrid p::tn1 plasmid psj5. properties of the resulting conjugative plasmid, psj15, indicated that bacteriophage vca1, like coliphage mu, can insert at many sites. by analogy with other hfr-like donors, the high-frequency, polarized chromosomal transfer mediated by plasmid psj15 in strain 162 appeared to depend on plasmid integration through the homologous dvca1 sequences in both replicons. wh ... | 1981 | 6260754 |
| transfer of polymyxin-resistance from bacillus polymyxa : transformation of gram-positive & gram-negative bacteria. | 1980 | 6260634 | |
| further evidence on the cytoplasmic existence of polymyxin resistance factor in vibrio cholerae-- "eltor". | 1980 | 6260631 | |
| [signal transfer from beta-adrenergic receptor to adenylate cyclase (author's transl)]. | hormonally stimulated adenylate cyclase of vertebrate cells is composed of at least three components which span the bilayer of the biological membrane: a hormone receptor, a gtp-binding coupling unit and a catalytic component synthesizing camp from atp. the coupling unit possesses gtpase activity enabling the hormonal signal to be delicately regulated. the molecular mechanism by which the enterotoxin of vibrio cholerae exerts its action, is an adp-ribosyl transfer to the gtp-binding component, w ... | 1980 | 6260124 |
| epidemiology of acute diarrheal disease. | 1980 | 6259574 | |
| receptor-specific large-scale purification of cholera toxin on silica beads derivatized with lysogm1 ganglioside. | 1. a receptor-specific affinity chromatographic method for large-scale purification of cholera toxin is described. the receptor ganglioside for cholera toxin, gm1, is hydrolysed to lysogm1 which is then covalently coupled, via stabilized schiff's bases, to porous silica beads (spherosil) onto which a layer of deae-dextran has been adsorbed and cross-linked before coupling. columns of these spherosil-deae-dextran-lysogm1 beads, in contrast to particles derivatized with lysoga1, bound the cholera ... | 1981 | 6258916 |
| [studies on antibacterial activity of cephotaxime (hr 756), a new antibiotic of the cephalosporin group (author's transl)]. | cephotaxime (hr 756) is a new cephalosporin antibiotic of wide spectrum and extraordinary activity, suitable for parenteral administration. it is highly resistant to the beta-lactamases of gram negative bacilli, being thus especially active against microorganisms such as indole-positive proteus, serratia marcencens, and others usually resistant to most beta-lactamic antibiotics because of their ability to produce beta-lactamases of high activity. the sensitivity of 355 different bacterial strain ... | 1980 | 6257990 |
| escherichia coli heat-labile enterotoxin genes are flanked by repeated deoxyribonucleic acid sequences. | the enterotoxin regions of the heat-labile and heat-stable enterotoxin (lt+ st+) plasmid, pjy11, originating in a clinically isolated escherichia coli strain, have been isolated as various-sized deoxyribonucleic acid (dna) fragments by using cloning vehicles. the structure of the lt+ region and its neighboring dna regions was studied by utilizing these recombinant plasmids. the lt+ region consisted of at least two genes, toxa and toxb, which could complement each other in trans. the toxa- and to ... | 1981 | 6257652 |
| [transposon-induced genetic rearrangements in the chromosome of vibrio el tor and their application to the construction of the first genetic map of a cholera vibrio]. | the specificity of integration of chloramphenicol resistance transposon (tn9) into vibrio el tor chromosome was studied. as shown by the isolation of auxotrophs offerring various nitritional requirements, tn9 displayed fairly low specificity in its interaction with the chromosome. when inserted into cholera vibrio chromosome, tn9 induced secondary rearrangements of genetic material. nutritional requirements were identified for the polyauxotrophic clones which resulted from the mutations in chrom ... | 1980 | 6257590 |
| [use of a transposon determining tetracycline resistance (tn10) in genetic studies of cholera vibrios]. | introduction of tetracycline-resistance transposon tn10 into rts1 (km), a plasmid which is temperature-sensitive for dna replication, made it possible to construct pns10 (km tc) plasmid used as a vehicle for transferring tn10 from escherichia coli to cholera vibrio v. el tor 218 as a result of intergeneric crosses. while being translocated from the plasmid genome to the chromosome of cholera vibrio transconjugants, tn10 was able of insertion into structural genes thus leading to the formation of ... | 1980 | 6257586 |
| the chemistry and biology of cholera toxin. | 1980 | 6256124 | |
| [genetic study of cholera vibrios]. | 1980 | 6255711 | |
| sendai virus utilizes specific sialyloligosaccharides as host cell receptor determinants. | purified sialyltransferases (cmp-n-acetyl-neuraminate:d-galactosyl-glycoprotein n-acetylneuraminyl-transferase, ec 2.4.99.1) in conjunction with neuraminidase (acylneuraminyl hydrolase, ec 3.2.1.18) were used to produce cell surface sialyloligosaccharides of defined sequence to investigate their role in paramyxovirus infection of host cells. infection of madin-darby bovine kidney cells by sendai virus was monitored by hemagglutination titer of the virus produced and by changes in morphological c ... | 1980 | 6255459 |
| polymyxin b release of unnicked cholera toxin subunit a. | polymyxin b treatment of vibrio cholerae 569b grown with or without lincomycin released an extracytoplasmic pool of free unnicked cholera toxin subunit a. | 1980 | 6254947 |
| attachment of sa-11 rotavirus to erythrocyte receptors. | treatment of human group o and sheep erythrocytes with receptor-destroying enzyme rendered them inagglutinable by simian rotavirus sa-11. the erythrocyte receptors were also removed by periodate oxidation and markedly reduced by incubation with a high concentration of trypsin, but they were not altered by infectivity-enhancing concentrations of trypsin, p-hydroxymercuribenzoate, or sodium sulfite (na2so3). hemagglutinating activity of the virus particles was destroyed by periodate oxidation at 3 ... | 1980 | 6253396 |
| comparative in vitro activities of cefotaxime and ceftizoxime (fk749): new cephalosporins with exceptional potency. | cefotaxime and its desacetoxymethyl derivative, ceftizoxime (previously known as fk749), are both extremely active against a wide spectrum of bacteria. in the present comparative study, the activity of ceftizoxime exceeded that of cefotaxime by a factor of four or more for strains of klebsiella, enterobacter, providencia, serratia, and bacteroides; the only species for which the activity of cefotaxime exceeded that of ceftizoxime by a factor of four was vibrio cholerae. against other species, th ... | 1980 | 6252829 |
| influence of various immunomodulators on the induction of experimental autoimmune encephalomyelitis in lewis rats. | 1980 | 6250977 | |
| the influence of glycosidases and lectins on insulin binding to zajdela hepatoma cells. | 1980 | 6250906 | |
| action of polymyxin b on liposomes prepared from cholera & el tor vibrios. | 1980 | 6249729 | |
| the ganglioside content of the milk fat-globule membrane and the mouse mammary-tumour virus isolated from the milk of infected mice. partial characterization of a new disialoganglioside. | the milk fat-globule membrane and the mouse mammary-tumour virus isolated from the milk of infected swiss mice have been investigated for their content in gangliosides. when compared on the lipid phosphorus basis, viral envelope is found to contain more than twice as much lipid-bound sialic acid as fat-globule membrane. the ganglioside patterns of these two structures appear rather similar, except for the occurrence in fat-globule membrane of a low ganglioside homolog, presumably gm2, not detect ... | 1980 | 6249587 |
| the polymyxin sensitivity and the phosphatidyl ethanolamine content of the vibrio cholerae membranes. | the phospholipid composition of three different strains of vibrio cholerae was determined by quantitative two-dimensional thin-layer chromatography. the polymyxin sensitivity of the whole organisms or of the liposomes derived from the total phospholipids of these organisms depended solely on the phosphatidyl ethanolamine content of the system concerned and could be quantitatively related by the equations y1 = 1.074 x1 - 9.828 and y2 = 1.22 x2 - 34.47 where y represents the maximum lysis (%)/hr a ... | 1980 | 6247314 |
| effects of clostridium difficile toxin on tissue-cultured cells. | a partially purified toxin of clostridium difficile induced similar morphologic changes in three different tissue-cultured mammalian cell lines. the morphologic changes were not associated with biochemical changes indentical to those caused by the enterotoxins of vibrio cholerae and escherichia coli. although the mechanisms responsible for the noncytotoxic morphologic effects remain to be delineated, the toxin appears to exert its effects by directly affecting membrane constituents. | 1980 | 6245152 |
| hydrolysis of gangliosides in micellar and liposomal dispersion by bacterial neuraminidases. | aqueous dispersions of pure gangliosides contain micelles of these compounds. in this dispersion state, the rates of hydrolysis of the neuraminyl residues by bacterial neuraminidases are slowest. incorporation of gangliosides into mixed dispersion with other lipids or into mixed micelles with bile salts considerably increases the reaction rates. the greatest reaction rates are obtained when di- or trisialogangliosides are incorporated into unilamellar vesicles of lecithin or sphingomyelin. | 1980 | 6244720 |
| hydrolysis of di- and trisialo gangliosides in micellar and liposomal dispersion by bacterial neuraminidases. | the hydrolysis of di- and trisialo gangliosides by bacterial neuraminidases was investigated. slow rates of hydrolysis were obtained with micellar dispersions of the pure gangliosides; the rates increased considerably with mixtures of ganglioside and phospholipids, such as phosphatidylcholine or sphingomyelin. the greatest rates of hydrolysis were obtained with mixtures containing 5-10 mol% ganglioside and 90-95% phospholipid. with the aid of the nonpenetrating reagent trinitrobenzenesulfonic ac ... | 1980 | 6243952 |
| [hemolytic activity of vibrio cholerae non-01]. | 1984 | 6239700 | |
| [bacteriocinogenicity of brucellae isolated in foci in the caucasus and their evaluation from taxonomic viewpoints]. | the aim of the study was to elucidate the possibility of using bacteriocinogenicity of brucella as taxonomic feature, to determine their phylogenetic relation to other microorganisms by their bacteriocinogenic properties and to investigate the physicochemical properties of brucellacin and conditions for its stable detection. the brucella cultures were isolated in the caucasus. investigation of their capacity for production of bacteriocin according to the procedure described by m.a. konstantinova ... | 1984 | 6230043 |
| etiological agents responsible for acute diarrhea in children in an urban community in burma. | 1983 | 6226849 | |
| [effect of the culture medium on the morphology of vibrio cholerae non-01]. | 1983 | 6225539 | |
| [oral rehydration therapy]. | 1983 | 6221741 | |
| in vitro antibacterial activity of norfloxacin (mk-0366, am-715) and other agents against gastrointestinal tract pathogens. | a comparison was made of the in vitro activities of norfloxacin and of nine other orally administered antibacterial agents against 180 clinical isolates representing the bacterial species most frequently implicated in infections of the gastrointestinal tract in humans. the 90% minimal inhibitory concentrations showed norfloxacin to be 4, 15, 4, 17, 17, 17, and 33 times more active than the next best compound tested against campylobacter fetus subsp. jejuni, escherichia coli, salmonella spp., shi ... | 1983 | 6219622 |
| [complex studies in the surveillance of the environmental vibrio flora in the city of gorki and gorki province]. | the analysis of the results of the comprehensive studies (phenotypic and genotypic properties, the precipitation test with the use of spontaneously agglutinating cultures, the determination of the antigenic structure by immunochemical analysis, etc.) carried out during the sanitary microbiological control of the environmental vibrioflora for the last 5 years are presented. only the combined use of the above-mentioned methods has allowed to ascertain the taxonomic position of atypical cultures. | 1982 | 6218723 |
| characterization of human peripheral blood t lymphocytes bearing receptors for autologous erythrocytes and t lymphocytes lacking these receptors. | when human t cells were treated with neuraminidase from vibrio cholerae, the capacity of t cells to form rosettes with autologous erythrocytes was markedly enhanced. the neuraminidase-treated t cells wee separated with autologous erythrocytes into autorosetting and nonrosetting cell populations, and these two populations examined for their reactivity to mitogens and b cells and for their regulatory activities. autorosetting t cells proliferated poorly in response to mitogens and autologous and a ... | 1982 | 6212591 |
| [influence of cultivation medium on the release and properties of lps from non-01 vibrio cholerae (author's transl)]. | 1982 | 6212135 | |
| identical 5s rrna nucleotide sequence of vibrio cholerae strains representing temporal, geographical, and ecological diversity. | enzymatic determination of the nucleotide sequence of 5s rrna prepared from a vibrio cholerae non-o1 serovar isolated from a canal in louisiana in 1978 revealed it to be identical to that of v. cholerae o1 isolated in 1905 from a pilgrim at the e1 tor quarantine station. the two strains are associated with sharply contrasting habitats. ranges of variation of 5 to 15% in procaryotic 5s rrna sequences between species are typical; variation within species may be 2% or greater. because the 5s rrna s ... | 1984 | 6206795 |
| investigation of populations heterogeneous according to o-antigen in vibrio cholerae cultures. | the o-antigenic composition of 36 cultures of vibrio cholerae agglutinating simultaneously with 01 cholera sera and 0 sera to nag vibrios of the sakazaki collection was investigated. it has been established experimentally that under the effect of medium and environmental conditions such cultures dissociate to subcultures differing in their affiliation to different serological groups according to 0 antigen. the passage of these cultures in the organism of susceptible animals promotes preservation ... | 1984 | 6206130 |
| [choleragen-anatoxin chemical cholera vaccine enriched with ogawa o-antigen]. | the cultural fluid of vibrio cholerae strains of serovar ogawa, grown under the conditions of submerged cultivation, has been shown to contain a large amount of soluble o-antigen which sharply differs from all other concomitant components in its molecular weight. by enriching the commercial chemical cholera vaccine known as choleragen toxoid with purified ogawa o-antigen a new preparation, consisting mainly of cholera toxoid and ogawa and inaba o-antigens and capable of producing pronounced immu ... | 1984 | 6204471 |
| serological studies on vibrio fluvialis. | the serology of 138 strains of vibrio fluvialis was studied. eighteen o-antigenic groups were defined among them and it was shown that the h antigens of all the strains were identical regardless of the biovar. the presence of mucoid antigen, which inhibits o agglutination, was found in some strains. as all o antisera for v. fluvialis contained some r antibody, all diagnostic o sera must be absorbed with r organisms before use. some o antigens of v. fluvialis were identical with those of certain ... | 1983 | 6202906 |
| immunological differences among the cholera/coli family of enterotoxins. | pure enterotoxins from two strains of vibrio cholerae and choleragen-related heat-labile enterotoxins (lts) from strains of escherichia coli of human and porcine origin were examined by ouchterlony-type immunodiffusion assays and by neutralization tests in y-1 adrenal cells using specific and immunopurified antisera. in accordance with previous findings, the results indicated that each of the toxins shared antigens with each of the others and that each, in addition, possessed unique antigenic de ... | 1983 | 6201315 |
| studies on the partial structure of the o-antigen of vibrio cholera ogawa g-2102. | detailed information was obtained regarding the partial structure of the lipopolysaccharide (lps), containing glucose, glucuronic acid, 2-amino-2-deoxy-glucose, l-glycero-d-gluco-heptose, and small proportions of l-glycero-d-manno-heptose, mannose, and galactose, isolated from vibrio cholera ogawa g-2102. structures of three oligosaccharides were determined. results of deamination experiments established the sequence of the linkages between the amino sugar and heptose residues in the o-antigenic ... | 1983 | 6200223 |
| [various characteristics of nag-vibrio isolated during the cold season]. | 1984 | 6199563 | |
| isolation of nontoxigenic vibrio cholerae o group 1 from a patient with severe gastrointestinal disease. | a nontoxigenic strain of vibrio cholerae o group 1 was isolated in florida from the stool of a patient with severe diarrhea. the strain had the same hemolytic and unique phage-sensitivity pattern as all toxigenic isolates from recent cases of cholera in texas and louisiana. identical strains were transiently isolated from sewerage systems in two other florida communities, suggesting that multiple human infections had occurred. this is the first indication that v. cholerae o1 strains which do not ... | 1984 | 6199370 |
| production of vibrio cholerae o1 and non-o1 typing sera in rabbits immunized with polysaccharide-protein carrier conjugates. | two systems are currently used to serologically type vibrio cholerae o1 and non-o1 isolates. antiserovar-serotype serum in the smith system is produced in rabbits immunized with live whole-cell vaccines, and that in the sakazaki system is produced in rabbits immunized with heat-killed vaccines. in neither system is the serovar-serotype-specific antigen clearly defined. during the course of a serological survey, ca. 10% of more than 2,500 v. cholerae isolates examined agglutinated in the optimal ... | 1984 | 6199368 |
| structure of the o-chain of the phenol-phase soluble cellular lipopolysaccharide of yersinia enterocolitica serotype o:9. | the phenol-phase soluble cellular lipopolysaccharide isolated by the phenol/water extraction method from yersinia enterocolitica serotype o:9 cells was shown by hydrolytic, periodate oxidation, methylation and nuclear magnetic resonance studies to be an s-type lipopolysaccharide with a linear o-antigenic polysaccharide of 1,2-linked 4,6-dideoxy-4-formamido-alpha-d-mannopyranosyl units. the serological cross-reactivity between y. enterocolitica serotype o:9 and the lipopolysaccharides of vibrio c ... | 1984 | 6199199 |
| [disc agglutination reactions for accumulation, isolation and identification of vibrio cholerae and vibrios not agglutinated by 01-serum]. | 1983 | 6196534 | |
| characterization of monoclonal antibodies that react with unique and cross-reacting determinants of cholera enterotoxin and its subunits. | seventeen selected hybridoma cell lines that produced monoclonal antibodies against cholera enterotoxin (ct) were isolated and characterized. all of the monoclonal antibodies contained the kappa light chain; 14 were of the immunoglobulin g1 (igg1) isotype and 3 were igg2a. the 17 monoclonal antibodies were divided into a minimum of seven different specificity groups based on their abilities to bind to the following purified test antigens in solid-phase radioimmunoassays: ct, the a and b polypept ... | 1983 | 6196297 |
| [titration of vibrio cholerae enterotoxin on the model of staphylococcus aureus 209 p. uv-2 mutant]. | 1983 | 6195442 | |
| [various features of the serotyping of vibrio cholerae el tor serologic variant inaba]. | 1983 | 6195419 | |
| prophylactic significance of the nonlipopolysaccharide antigens of vibrio cholerae. | the infant-mouse cholera model was used for evaluation of the immunoprophylactic significance of the nonlipopolysaccharide antigens of vibrio cholerae. the protective efficiency of antibodies to the nonlipopolysaccharide components of the 569b strain was much greater than that of antibodies to the lipopolysaccharide. protective nonlipopolysaccharide antigens were not detected in two other strains, however; the possible basis for this restricted distribution was considered in light of studies fro ... | 1983 | 6195273 |
| biological activities of enterotoxin from vibrio cholerae non-01. | the specific antiserum 54(2) prepared in rabbits by combined intraperitoneal and intravenous immunization with the enterotoxin from vibrio cholerae non-01 was found to neutralize the enterotoxin biologic activity expressed by rabbit skin vascular permeability and hemorrhage factors. the inhibition of biologic activity was also observed when using antiserum to choleragen, but choleragen could not be neutralized by specific serum 54(2). the enterotoxin was also inactivated when heated at 100 degre ... | 1983 | 6193168 |
| studies on alkaline phosphatase, catalase and z-galactosidase in vibrio el tor under normal and rifampicin resistant conditions. | alkaline phosphate, catalase and beta-galactosidase activities of vibrio et tor were decreased after acquisition of resistance towards rifampicin. zn2+, mn2+ and edta inhibited alkaline phosphatase which is most active with p-nitrophenylphosphate as substrate while mg2+ was found to suppress alkaline phosphatase activity. removal of edta however, restores the original activity. rifampicin could not induce mutation of lactose nonfermenting vibrio el for cells allowing them to grow on lactose as s ... | 1982 | 6189376 |
| purification and some properties of a non-o1 vibrio cholerae enterotoxin that is identical to cholera enterotoxin. | cholera-like enterotoxin was isolated and purified from the culture supernatant of a non-o1 strain of vibrio cholerae, e8498, isolated from the environment. enterotoxin was purified by aluminum hydroxide absorption and elution and successive gel filtrations on sephadex g-100, bio-gel a-5m, and sephadex g-75. purified enterotoxin gave a single stained band on polyacrylamide gel disc electrophoresis, and the mobility was the same as that of cholera enterotoxin. the specific biological activity of ... | 1983 | 6188694 |
| immunochemical analysis of the determinant recognized by a monoclonal antibody (mbr1) which specifically binds to human mammary epithelial cells. | a monoclonal antibody (mbr1) raised against a membrane preparation (cm) of a human breast cancer line (mcf-7) and characterized as mammary gland epithelium associated (s. mènard, e. tagliabue, s. canevari, g. fossati, and m. i. colnaghi. generation of monoclonal antibodies reacting with normal and cancer cells of human breast. cancer res., 43:1295-1300, 1983), was used to biochemically define and partially purify its target antigen. the antigenic activity recognized by mbr1 was unaffected by tre ... | 1983 | 6186373 |
| [immunoenzyme complex based on a polyfunctional carrier for identification and detection of microorganisms]. | 1982 | 6185754 | |
| mapping of a gene in vibrio cholerae that determines the antigenic structure of cholera toxin. | in ouchterlony-type immunodiffusion gels, the cholera toxin produced by the classical vibrio cholerae strain 569b was indistinguishable from the cholera toxin of the eltor strain rj1. however, the cholera toxin produced by both of these strains was incompletely cross-reactive with the cholera toxin produced by the eltor strain 3083-2. the allele of the gene responsible for the 569b and rj1 type of toxin was designated vct-1, and the allele responsible for the 3083-2 type of toxin was designated ... | 1982 | 6185422 |
| [improved method of releasing the phage from pseudolysogenic clones of vibrio cholerae]. | 1982 | 6184536 | |
| monoclonal antibodies against vibrio cholerae lipopolysaccharide. | a cell line producing monoclonal antibodies directed against the core region of vibrio cholerae lipopolysaccharide has been established. these antibodies were inhibited by lipopolysaccharide preparations of both o-group 1 vibrios and some non-o-group 1 vibrios as detected in enzyme-linked immunosorbent assay-inhibition experiments. coagglutination experiments with monoclonal and polyclonal antibodies adsorbed to protein a-carrying staphylococci were performed. all v. cholerae strains tested, reg ... | 1982 | 6183214 |
| quality of antisera used in the diagnosis of cholera. | 1982 | 6181361 | |
| serological comparison of two collections of vibrio cholerae non o1. | two large, independently obtained collections of vibrio cholerae non o1, containing 59 and 67 reference strains, respectively, were compared serologically in four laboratories. twenty strains in each collection were considered identical, and an additional 16 strains in each collection were probably identical. twenty-eight unique strains were identified, and inconclusive results were obtained with 25 strains. nine strains were not considered v. cholerae in at least one testing laboratory. of thes ... | 1982 | 6181089 |
| [nonselective mechanism of antibody-induced stable antigenic variability of microorganisms. iii. suggested mechanism of variability of certain microorganisms]. | 1982 | 6178238 | |
| induction by cholera toxin of synchronous divisions in vivo in the epidermis resulting in hyperplasia. | intracutaneous injection of cholera toxin, exotoxin of vibrio cholerae, into the dorsal skin of mice, rats, and hamsters at doses of greater than 0.1 ng evoked an acute reaction at the site of injection, which was characterized histologically by an edematous reaction in the dermis and mitotic stimulation in the epidermis. mitotic and labeling induces of basal cells of the mouse epidermis showed two peaks at 24 and 48 hr after injection, thereby producing epidermal hyperplasia. the thickness of t ... | 1981 | 6171825 |
| use of the india ink immuno-reaction for the rapid detection of enteric pathogens in two area of nigeria. | the india-ink immuno-reaction (iir) was used as a simple, convenient procedure for the detection of carriers of enteric organisms in an unselected sample of patients attending a mission clinic at kubacha in a remote area of kaduna state, northern nigeria. to assess the reliability of this procedure in difficult working conditions a similar population from the same clinic was subsequently examined by routine bacteriological culture techniques. because of a temporary shortage of suitable anti-sera ... | 1981 | 6171592 |
| isolation of special antibodies which react only with homologous enterotoxins from vibrio cholerae and enterotoxigenic escherichia coli. | cholera enterotoxin (ct) and heat-labile enterotoxin (lt) produced by enterotoxigenic escherichia coli share a common antigenic determinant. in addition, ct and lt each have a unique antigenic determinant. antisera were prepared by immunoaffinity chromatography against these unique antigenic determinants, that is, antiserum that reacted with ct but not with lt and antiserum that reacted with lt but not with ct. antiserum against the common antigenic determinant to ct and lt was also prepared by ... | 1981 | 6171516 |
| [epidemiology of cholera in the world. development between 1970 and 1980]. | the authors present a general review of ten year evolution of the 7th pandemic of cholerae. they discuss the official data known from the country declarations. from the epidemiological features they distinguish a hydric dilution model of cholerae (few cases, few carriers, endemicity) and a direct inter-human contamination type (epidemic outbreaks, no endemicity). | 1981 | 6170470 |
| [determination of vibrio cholerae enterotoxin by the passive immune hemolysis technic]. | to determine the antigenic determinants of cholera toxin, the passive immune hemolysis test was used. this test, proved to be highly sensitive (100-200 pg/ml) and specific, yielded results quicker than all other immunological methods for the determination of cholera toxin. the study of 36 cholera and nag-vibrio strains revealed that v. cholerae synthesized the greatest amount of the toxin, whereas v. eltor formed a heterogenous group, comprising strains capable of synthesizing the toxin in consi ... | 1981 | 6168143 |
| different cell-surface receptor determinants of antigenically similar influenza virus hemagglutinins. | two influenza virus substrains, a/ri/5-/57 and a/ri/5+/57, with antigenically similar hemagglutinins and neuraminidases but with different properties of elution from erythrocytes, have been examined for the specificity of their interaction with cell surface sialyloligosaccharides. this was accomplished by using erythrocytes treated with vibrio cholerae neuraminidase to remove sialic acids, and then modified with cmp-neuac and three purified sialyltransferases to contain either the neuac alpha 2, ... | 1981 | 6167577 |
| enzymically-mediated changes in murine mammary adenocarcinoma cell membrane induces changes in lymphoid tissue immune ribonucleic acids. | a cell suspension derived from a single murine spontaneous mammary adenocarcinoma was resolved on a linear gradient of ficoll, into twelve distinct neoplastic cell subpopulation. a second cell suspension, also derived from a single murine mammary adenocarcinoma was first treated with vibrio cholera neuraminidase (vcn) then was resolved on an identical gradient of ficoll into twelve distinct subpopulation. each cell population was seeded and allowed to proliferate. the cell subpopulations differe ... | 1980 | 6165333 |
| characterization of vibrio cholerae protease activities with peptide digest analysis. | a simple method for the analysis of microbial proteases is described that was used to characterize the proteolytic activities of various vibrio cholerae isolates. this method utilized the unique peptides generated from the degradation of a standard protein by proteases of various specificities. these peptides were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. the unique patterns of peptides seen in gels can be used to type proteases according to their relative specificit ... | 1981 | 6161944 |
| receptor mediated gonadotropin action in the ovary. modulation of progesterone response in isolated rat ovarian cells by gonadotrophin, cholera enterotoxin and cyclic nucleotides: requirement for rna and protein synthesis. | 1980 | 6161508 |