Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| probiotics down-regulate flaa sigma28 promoter in campylobacter jejuni. | lactobacilli and bifidobacteria are important members of the gastrointestinal microflora of humans and animals and are thought to have positive effects on human health. therefore, there is an increasing interest in using these microorganisms as probiotics to be incorporated into either fermented dairy products or tablets. however, convincing scientific data that support claims of their health benefits are scarce. the effect of cell-free extracts of milk fermented by 10 probiotic bacteria (five b ... | 2005 | 16300065 |
| neurological complications in kidney transplant recipients. | neurological complications are frequent in renal transplant recipients and may largely contribute to morbidity and mortality. the postransplant neurological complications may be categorized into five areas: 1) immunosuppressive medications, 2) stroke, 3) peripheral neuropathies, 4) infection, and 5) malignancies. a number of complications are directly caused by the neurotoxicity of immunosuppressive agents. calcineurin-inhibitors may cause mild symptoms, such as tremors and paresthesia, or sever ... | 2005 | 16299677 |
| evidence of a large novel gene pool associated with prokaryotic genomic islands. | microbial genes that are "novel" (no detectable homologs in other species) have become of increasing interest as environmental sampling suggests that there are many more such novel genes in yet-to-be-cultured microorganisms. by analyzing known microbial genomic islands and prophages, we developed criteria for systematic identification of putative genomic islands (clusters of genes of probable horizontal origin in a prokaryotic genome) in 63 prokaryotic genomes, and then characterized the distrib ... | 2005 | 16299586 |
| iron and fur regulation in vibrio cholerae and the role of fur in virulence. | regulation of iron uptake and utilization is critical for bacterial growth and for prevention of iron toxicity. in many bacterial species, this regulation depends on the iron-responsive master regulator fur. in this study we report the effects of iron and fur on gene expression in vibrio cholerae. we show that fur has both positive and negative regulatory functions, and we demonstrate fur-independent regulation of gene expression by iron. nearly all of the known iron acquisition genes were repre ... | 2005 | 16299312 |
| mouse intestine selects nonmotile flhdc mutants of escherichia coli mg1655 with increased colonizing ability and better utilization of carbon sources. | d-gluconate which is primarily catabolized via the entner-doudoroff (ed) pathway, has been implicated as being important for colonization of the streptomycin-treated mouse large intestine by escherichia coli mg1655, a human commensal strain. in the present study, we report that an mg1655 deltaedd mutant defective in the ed pathway grows poorly not only on gluconate as a sole carbon source but on a number of other sugars previously implicated as being important for colonization, including l-fucos ... | 2005 | 16299298 |
| community-based safety, immunogenicity, and transmissibility study of the shigella sonnei wrss1 vaccine in israeli volunteers. | we describe the first community-based evaluation of shigella sonnei strain wrss1, a live, oral candidate vaccine attenuated by a 212-bp deletion in the virg (or icsa) plasmid virulence gene. three single-dose regimens of wrss1 (5 x 10(3) cfu, 2 x 10(4) cfu, and 4 x 10(5) cfu) were tested with cohorts of 15 adult volunteers. the vaccine was generally well tolerated at the 10(3)- and 10(4)-cfu doses. there were no fevers and there was one report of moderate diarrhea in 30 vaccinees; five additiona ... | 2005 | 16299296 |
| influence of pilin glycosylation on pseudomonas aeruginosa 1244 pilus function. | the opportunistic pathogen pseudomonas aeruginosa is a leading cause of nosocomial pneumonia. among its virulence factors, the type iv pili of p. aeruginosa strain 1244 contain a covalently linked, three-sugar glycan of previously unknown significance. the work described in this paper was carried out to determine the influence of the p. aeruginosa 1244 pilin glycan on pilus function, as well as a possible role in pathogenesis. to accomplish this, a deletion was introduced into the pilo gene of t ... | 2005 | 16299283 |
| induction of thioredoxin is required for nodule development to reduce reactive oxygen species levels in soybean roots. | nodules are formed on legume roots as a result of signaling between symbiotic partners and in response to the activities of numerous genes. we cloned fragments of differentially expressed genes in spot-inoculated soybean (glycine max) roots. many of the induced clones were similar to known genes related to oxidative stress, such as thioredoxin and beta-carotene hydroxylase. the deduced amino acid sequences of full-length soybean cdnas for thioredoxin and beta-carotene hydroxylase were similar to ... | 2005 | 16299179 |
| successful antibiotic treatment of helicobacter pylori negative gastric mucosa associated lymphoid tissue lymphomas. | the role of antibiotic treatment in early stage gastric mucosa associated lymphoid tissue (malt) lymphoma not associated with helicobacter pylori infection has not been investigated. | 2006 | 16299027 |
| evaluation of rapid diagnostic methods for pediatric viral diarrhea using samples collected in nepal and japan. | we evaluated real time pcr, elisa and immunochromatography (icg) test systems for detection of diarrheagenic viruses (rotavirus, adenovirus and norovirus) in pediatric diarrheal fecal samples collected in kathmandu, nepal (n = 23) and in kobe, japan (n = 25) in summer of 2004. of the 23 samples collected in nepal, one (4.3%) was positive for rotavirus and two (8.7%) for adenovirus whereas six out of 25 samples (24.0%) collected in japan were positive for norovirus. of the japanese samples, 11 (4 ... | 2004 | 16295734 |
| a guild of 45 crispr-associated (cas) protein families and multiple crispr/cas subtypes exist in prokaryotic genomes. | clustered regularly interspaced short palindromic repeats (crisprs) are a family of dna direct repeats found in many prokaryotic genomes. repeats of 21-37 bp typically show weak dyad symmetry and are separated by regularly sized, nonrepetitive spacer sequences. four crispr-associated (cas) protein families, designated cas1 to cas4, are strictly associated with crispr elements and always occur near a repeat cluster. some spacers originate from mobile genetic elements and are thought to confer "im ... | 2005 | 16292354 |
| ambiguous value of haemophilus influenzae isolation in guillain-barre and fisher syndromes. | haemophilus influenzae is considered a causative agent of guillain-barré syndrome (gbs) and fisher syndrome, but the frequency of this infection in gbs is controversial. | 2005 | 16291907 |
| structural basis for shikimate-binding specificity of helicobacter pylori shikimate kinase. | shikimate kinase (ec 2.7.1.71) catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid in the presence of atp. as the fifth key step in the shikimate pathway for aromatic amino acid biosynthesis in bacteria, fungi, and plants, but not mammals, shikimate kinase represents an attractive target for the development of new antimicrobial agents, herbicides, and antiparasitic agents. here, we report the 1.8-angstroms crystal structure of helicobacter pylori shikimate kinase (hps ... | 2005 | 16291688 |
| characterization of temporal protein production in pseudomonas aeruginosa biofilms. | phenotypic and genetic evidence supporting the notion of biofilm formation as a developmental process is growing. in the present work, we provide additional support for this hypothesis by identifying the onset of accumulation of biofilm-stage specific proteins during pseudomonas aeruginosa biofilm maturation and by tracking the abundance of these proteins in planktonic and three biofilm developmental stages. the onset of protein production was found to correlate with the progression of biofilms ... | 2005 | 16291684 |
| involvement of senc in assembly of cytochrome c oxidase in rhodobacter capsulatus. | senc, a sco1 homolog found in the purple photosynthetic bacteria, has been implicated in affecting photosynthesis and respiratory gene expression, as well as assembly of cytochrome c oxidase. in this study, we show that senc from rhodobacter capsulatus is involved in the assembly of a fully functional cbb(3)-type cytochrome c oxidase, as revealed by decreased cytochrome c oxidase activity in a senc mutant. we also show that a putative copper-binding site in senc is required for activity and that ... | 2005 | 16291681 |
| campylobacter fetus bacteraemia in a healthy individual: clinical and therapeutical implications. | campylobacter species are well-recognized common causes of gastrointestinal infections. while campylobacter jejuni is probably the most common campylobacter isolated in humans, campylobacter fetus is rather infrequent and mostly related with bacteraemia. even on such occasions, it seems that immunocompetent individuals are spared. we report a case of c. fetus bacteraemia in a healthy-except for impaired fasting glucose (ifg) levels-farmer, presenting as an acute febrile syndrome and treated succ ... | 2005 | 16291287 |
| characterization of heterologous hemoglobin and flavohemoglobin promoter regulation in escherichia coli. | bacterial hemoglobins and flavohemoglobins have been used to improve cell growth and productivity in biotechnological applications. the expression of globin genes can be induced by reducing the oxygen supply or applying external stressors, which provide a simple and inexpensive mechanism for induction of heterologous protein production. it is in the interest of the biotechnological industry to seek new promoters, which are non-patented, cheap and simple to induce. therefore, new globin gene prom ... | 2006 | 16290305 |
| campylobacter jejuni loss of culturability in aqueous microcosms and ability to resuscitate in a mouse model. | water is known as one of the main transmission routes of campylobacter and contributes to increase the number of sporadic infections and outbreaks. campylobacter jejuni persists in the environment, especially in water, in a viable but non-culturable (vbnc) form that is thought to be a possible cause of water-borne outbreaks. in this study, we evaluated the loss of culturability and viability of 9 c. jejuni strains of clinical origin and one atcc reference strain when kept at 4 degrees c in artif ... | 2006 | 16290304 |
| crystal structure of virulence factor cj0248 from campylobacter jejuni at 2.25 a resolution reveals a new fold. | 2006 | 16287129 | |
| glycosylation substrate specificity of pseudomonas aeruginosa 1244 pilin. | the beta-carbon of the pseudomonas aeruginosa 1244 pilin c-terminal ser is a site of glycosylation. the present study was conducted to determine the pilin structures necessary for glycosylation. it was found that although thr could be tolerated at the pilin c terminus, the blocking of the ser carboxyl group with the addition of an ala prevented glycosylation. pilin from strain pa103 was not glycosylated by p. aeruginosa 1244, even when the c-terminal residue was converted to ser. substituting th ... | 2006 | 16286455 |
| glycosylation substrate specificity of pseudomonas aeruginosa 1244 pilin. | the beta-carbon of the pseudomonas aeruginosa 1244 pilin c-terminal ser is a site of glycosylation. the present study was conducted to determine the pilin structures necessary for glycosylation. it was found that although thr could be tolerated at the pilin c terminus, the blocking of the ser carboxyl group with the addition of an ala prevented glycosylation. pilin from strain pa103 was not glycosylated by p. aeruginosa 1244, even when the c-terminal residue was converted to ser. substituting th ... | 2006 | 16286455 |
| functional characterization of dehydratase/aminotransferase pairs from helicobacter and campylobacter: enzymes distinguishing the pseudaminic acid and bacillosamine biosynthetic pathways. | helicobacter pylori and campylobacter jejuni have been shown to modify their flagellins with pseudaminic acid (pse), via o-linkage, while c. jejuni also possesses a general protein glycosylation pathway (pgl) responsible for the n-linked modification of at least 30 proteins with a heptasaccharide containing 2,4-diacetamido-2,4,6-trideoxy-alpha-d-glucopyranose, a derivative of bacillosamine. to further define the pse and bacillosamine biosynthetic pathways, we have undertaken functional character ... | 2006 | 16286454 |
| large-scale genetic variation of the symbiosis-required megaplasmid psyma revealed by comparative genomic analysis of sinorhizobium meliloti natural strains. | sinorhizobium meliloti is a soil bacterium that forms nitrogen-fixing nodules on the roots of leguminous plants such as alfalfa (medicago sativa). this species occupies different ecological niches, being present as a free-living soil bacterium and as a symbiont of plant root nodules. the genome of the type strain rm 1021 contains one chromosome and two megaplasmids for a total genome size of 6 mb. we applied comparative genomic hybridisation (cgh) on an oligonucleotide microarrays to estimate ge ... | 2005 | 16283928 |
| viral etiology and incidence of acute gastroenteritis in young children attending day-care centers. | the purpose of the study was to investigate the frequency, morbidity and cause of acute gastroenteritis in children attending day-care centers in denmark. | 2005 | 16282929 |
| preferential attachment in the evolution of metabolic networks. | many biological networks show some characteristics of scale-free networks. scale-free networks can evolve through preferential attachment where new nodes are preferentially attached to well connected nodes. in networks which have evolved through preferential attachment older nodes should have a higher average connectivity than younger nodes. here we have investigated preferential attachment in the context of metabolic networks. | 2005 | 16281983 |
| infections and autoimmune diseases. | the high percentage of disease-discordant pairs of monozygotic twins demonstrates the central role of environmental factors in the etiology of autoimmune diseases. efforts were first focussed on the search for triggering factors. the study of animal models has clearly shown that infections may trigger autoimmune diseases, as in the case of coxsackie b4 virus in type i diabetes and the encephalomyocarditis virus in autoimmune myositis, two models in which viruses are thought to act by increasing ... | 2005 | 16278064 |
| [guillain-barré syndrome associated campylobacter jejuni serogroup penner a showing antecedent fever and abdominal pain without diarrhea]. | we reported a 28-year-old woman who had guillain-barré syndrome (gbs). eight days after fever up and abdominal pain, she had weakness and dysesthesia of both hands and feet. campylobacter jejuni (c. jejuni) was isolated from her stool culture without diarrhea. igg anti-gm1 antibody was detected in her serum. nerve conduction studies revealed a predominantly axonal neuropathy. after intravenous immunoglobulin therapy, her symptoms promptly improved. penner 19 of c. jejuni was isolated more freque ... | 2005 | 16277234 |
| a way out of the maze: campylobacter jejuni gene polymorphisms define guillain-barré syndrome. | 2005 | 16275819 | |
| an analysis of the feasibility of short read sequencing. | several methods for ultra high-throughput dna sequencing are currently under investigation. many of these methods yield very short blocks of sequence information (reads). here we report on an analysis showing the level of genome sequencing possible as a function of read length. it is shown that re-sequencing and de novo sequencing of the majority of a bacterial genome is possible with read lengths of 20-30 nt, and that reads of 50 nt can provide reconstructed contigs (a contiguous fragment of se ... | 2005 | 16275781 |
| campylobacter coli in swine production: antimicrobial resistance mechanisms and molecular epidemiology. | the aim of this study was to determine antimicrobial resistance, to evaluate and compare the use of two genotyping methods for molecular epidemiology purposes, and to determine the genotypic diversity of campylobacter coli of porcine origin. a total of 100 c. coli isolates from swine were tested for susceptibility to six antimicrobials using the agar dilution method and genotyped using two high-resolution fingerprinting approaches: multilocus sequence typing (mlst) and pulsed-field gel electroph ... | 2005 | 16272508 |
| specific and sensitive detection of neisseria gonorrhoeae in clinical specimens by real-time pcr. | early diagnosis of neisseria gonorrhoeae infections is important with regard to patients' health and infectivity. we report the development of a specific and sensitive taqman assay for the detection of n. gonorrhoeae in clinical samples. the target sequence is a 76-bp fragment of the 5' untranslated region of the opa genes that encode opacity proteins. a panel of 448 well-defined n. gonorrhoeae isolates was used to evaluate and optimize the assay. the method employs two minor-groove binding prob ... | 2005 | 16272500 |
| guillain-barré syndrome. | guillain-barré syndrome consists of at least four subtypes of acute peripheral neuropathy. major advances have been made in understanding the mechanisms of some of the subtypes. the histological appearance of the acute inflammatory demyelinating polyradiculoneuropathy (aidp) subtype resembles experimental autoimmune neuritis, which is predominantly caused by t cells directed against peptides from the myelin proteins p0, p2, and pmp22. the role of t-cell-mediated immunity in aidp remains unclear ... | 2005 | 16271648 |
| carbohydrate mimicry of campylobacter jejuni lipooligosaccharide is critical for the induction of anti-gm1 antibody and neuropathy. | the expression of ganglioside-mimicking structures of campylobacter jejuni lipooligosaccharides (los) is considered essential for the induction of antiganglioside antibodies that lead to guillain-barré syndrome (gbs). the gale gene in c. jejuni is involved in the biosynthesis of the los outer-core oligosaccharide structures. we have demonstrated that the c. jejuni hb9313 (hs:19) parental strain expresses a los structure containing gm1-like epitopes, and the c. jejuni knockout mutant of the gale ... | 2006 | 16270308 |
| design, validation, and application of a seven-strain staphylococcus aureus pcr product microarray for comparative genomics. | bacterial comparative genomics has been revolutionized by microarrays, but the power of any microarray is dependent on the number and diversity of gene reporters it contains. staphylococcus aureus is an important human pathogen causing a wide range of invasive and toxin-mediated diseases, and more than 20% of the genome of any isolate consists of variable genes. seven whole-genome sequences of s. aureus are available, and we exploited this rare opportunity to design, build, and validate a compre ... | 2005 | 16269792 |
| enzymatic and genetic characterization of carbon and energy metabolisms by deep-sea hydrothermal chemolithoautotrophic isolates of epsilonproteobacteria. | the carbon and energy metabolisms of a variety of cultured chemolithoautotrophic epsilonproteobacteria from deep-sea hydrothermal environments were characterized by both enzymatic and genetic analyses. all the epsilonproteobacteria tested had all three key reductive tricarboxylic acid (rtca) cycle enzymatic activities--atp-dependent citrate lyase, pyruvate:ferredoxin oxidoreductase, and 2-oxoglutarate:ferredoxin oxidoreductase--while they had no ribulose 1,5-bisphosphate carboxylase (rubisco) ac ... | 2005 | 16269773 |
| development and application of real-time pcr assays for quantification of genes encoding tetracycline resistance. | we report here the development, validation, and use of three real-time pcr assays to quantify the abundance of the following three groups of tetracycline resistance genes: tet(a) and tet(c); tet(g); and tet genes encoding ribosomal protection proteins, including tet(m), tet(o), tetb(p), tet(q), tet(s), tet(t), and tet(w). the assays were validated using known numbers of sample-derived tet gene templates added to microbiome dna. these assays are both precise and accurate over at least 6 log tet g ... | 2005 | 16269727 |
| intervening sequence acquired by lateral gene transfer in tropheryma whipplei results in 23s rrna fragmentation. | completion of tropheryma whipplei genome sequencing may provide insights into the evolution of the molecular mechanisms underlying the pathogenicity of this microorganism. the first postgenomic application was the successful design of a comprehensive culture medium that allows axenic growth of this bacterium, which is particularly recalcitrant to cultivation. this achievement in turn permitted analysis of t. whipplei rna without contaminating eukaryotic nucleic acids. to obtain high-quality rna, ... | 2005 | 16269699 |
| bacteriophage therapy to reduce campylobacter jejuni colonization of broiler chickens. | colonization of broiler chickens by the enteric pathogen campylobacter jejuni is widespread and difficult to prevent. bacteriophage therapy is one possible means by which this colonization could be controlled, thus limiting the entry of campylobacters into the human food chain. prior to evaluating the efficacy of phage therapy, experimental models of campylobacter colonization of broiler chickens were established by using low-passage c. jejuni isolates hpc5 and giic8 from united kingdom broiler ... | 2005 | 16269681 |
| bile-salt-stimulated lipase and mucins from milk of 'secretor' mothers inhibit the binding of norwalk virus capsids to their carbohydrate ligands. | breast-feeding-associated protection against calicivirus diarrhoea is associated with the presence of high levels of 2-linked oligosaccharides in mother's milk, and human calicivirus strains including the nv (norwalk virus) use gut 2-linked fucosylated glycans as receptors, suggesting the presence of decoy receptors in milk. our aim was to analyse the ability of human milk to inhibit the attachment of rnv vlps (recombinant nv-like particles) to their carbohydrate ligands and to characterize pote ... | 2006 | 16266293 |
| dissimilatory metabolism of nitrogen oxides in bacteria: comparative reconstruction of transcriptional networks. | bacterial response to nitric oxide (no) is of major importance since no is an obligatory intermediate of the nitrogen cycle. transcriptional regulation of the dissimilatory nitric oxides metabolism in bacteria is diverse and involves fnr-like transcription factors hcpr, dnr, and nnrr; two-component systems narxl and narqp; no-responsive activator norr; and nitrite-sensitive repressor nsrr. using comparative genomics approaches, we predict dna-binding motifs for these transcriptional factors and ... | 2005 | 16261196 |
| immunoglobulin km allotypes are associated with the prevalence of autoantibodies to gd1a ganglioside, but not with susceptibility to the disease, in japanese patients with guillain-barré syndrome. | guillain-barré syndrome (gbs), an autoimmune disease of the peripheral nervous system, is associated with antecedent campylobacter jejuni infection. gm and km allotypes--genetic markers of immunoglobulin gamma and kappa chains, respectively--are implicated in the etiopathogenesis of several autoimmune diseases. to determine if gm/km phenotypes are associated with gbs and influence antibody responses to c. jejuni and to gm1 and gd1a gangliosides, 72 japanese gbs patients and 73 controls were allo ... | 2005 | 16258760 |
| evaluation of antibiotic treatment against "candidatus helicobacter suis" in a mouse model. | "helicobacter heilmannii" (proposed name) type 1 colonizes the human stomach. it has been shown to be identical to "candidatus helicobacter suis," a helicobacter species colonizing the stomachs of >60% of slaughter pigs. this bacterium has not been isolated in vitro until now. antibiotic susceptibility testing of "candidatus helicobacter suis" has not been carried out so far. for the present study, a mouse model was adopted to evaluate the antibiotic susceptibility of this organism. mice infecte ... | 2005 | 16251292 |
| [study of real-time pcr assays for rapid detection of food-borne pathogens]. | a duplex real-time sybr green lightcycler pcr (lc-pcr) assay with dna extraction using qiaamp dna stool minikit was evaluated for detection of 8 of 19 species of food-borne pathogens, including plesiomonas shigelloides, providencia alcalifaciens, in five stool specimens. the time frame was within 2h or less. the protocol used the same lc-pcr with 22 pairs of specific primers. the rapid amplification and reliable detection of specific genes were determined by this lc-pcr assay from 10 cases of fo ... | 2005 | 16248373 |
| epitope shared by functional variant of organic cation/carnitine transporter, octn1, campylobacter jejuni and mycobacterium paratuberculosis may underlie susceptibility to crohn's disease at 5q31. | campylobacter jejuni and mycobacterium paratuberculosis have been implicated in the pathogenesis of crohn's disease. the presence of bacterial metabolites in the colonic lumen causing a specific breakdown of fatty acid oxidation in colonic epithelial cells has been suggested as an initiating event in inflammatory bowel disease (ibd). l-carnitine is a small highly polar zwitterion that plays an essential role in fatty acid oxidation and atp generation in intestinal bioenergetic metabolism. the or ... | 2005 | 16246312 |
| reduction in flock prevalence of campylobacter spp. in broilers in norway after implementation of an action plan. | an action plan against thermophilic campylobacter spp. in norwegian broilers was implemented in may 2001. the action plan consists of three parts: a surveillance program including all norwegian broiler flocks slaughtered before 50 days of age, a follow-up advisory service on farms delivering flocks positive for campylobacter spp., and surveys of broiler meat products at the retail level. this article presents results covering the inclusive 3-year period between 2002 and 2004. during this period, ... | 2005 | 16245734 |
| a robotic dna purification protocol and real-time pcr for the detection of campylobacter jejuni in foods. | primers designed to amplify a campylobacter jejuni cadf gene sequence were used in an sybr green i real-time pcr assay as an alternative to conventional bacteriological methods for the rapid detection of c. jejuni in foods. twenty-five grams of chicken skin (breast and thigh) was contaminated by adding approximately 1, 10, or 50 cfu of c. jejuni atcc 35560. twenty-five grams of pork and 25-ml aliquots of milk were also inoculated with 1 and 10 cfu of the pathogen. the samples were incubated in b ... | 2005 | 16245718 |
| [recurrent miller-fisher syndrome]. | miller-fisher syndrome (mfs) is a rare auto-immune post-infectious syndrome, characterized by an ataxia, an ophthalmoplegia and a generalized areflexia. it is considered as a clinical variant of guillain-barré syndrome (gbs). mfs is correlated with the presence of anti-gq1b antibodies, elevated cerebrospinal fluid (csf) protein levels, presence of mostly sensitive electrophysiological abnormalities and for some authors central involvement with increased signal intensity of brainstem and cerebell ... | 2005 | 16244569 |
| enzymatic synthesis of cytidine 5'-monophospho-n-acetylneuraminic acid. | we have established an efficient method for enzymatic production of cytidine 5'-monophospho-n-acetylneuraminic acid (cmp-neuac) from inexpensive materials, n-acetylglucosamine (glcnac) and cytidine 5'-monophosphate (cmp). the haemophilus influenzae nane gene encoding glcnac 6-phosphate (glcnac 6-p) 2-epimerase and the campylobacter jejuni neub1 gene encoding n-acetylneuraminic acid (neuac) synthetase, both of whose products are involved in neuac biosynthesis, were cloned and co-expressed in esch ... | 2005 | 16244446 |
| a study of archaeal enzymes involved in polar lipid synthesis linking amino acid sequence information, genomic contexts and lipid composition. | cellular membrane lipids, of which phospholipids are the major constituents, form one of the characteristic features that distinguish archaea from other organisms. in this study, we focused on the steps in archaeal phospholipid synthetic pathways that generate polar lipids such as archaetidylserine, archaetidylglycerol, and archaetidylinositol. only archaetidylserine synthase (ass), from methanothermobacter thermautotrophicus, has been experimentally identified. other enzymes have not been fully ... | 2005 | 16243780 |
| bordetella bronchiseptica flagellin is a proinflammatory determinant for airway epithelial cells. | motility is an important virulence phenotype for many bacteria, and flagellin, the monomeric component of flagella, is a potent proinflammatory factor. of the three bordetella species, bordetella pertussis and bordetella parapertussis are nonmotile human pathogens, while bordetella bronchiseptica expresses flagellin and causes disease in animals and immunocompromised human hosts. the bvgas two-component signal transduction system regulates phenotypic-phase transition (bvg+, bvg-, and bvg(i)) in ... | 2005 | 16239555 |
| intestinal innate immunity to campylobacter jejuni results in induction of bactericidal human beta-defensins 2 and 3. | campylobacter jejuni is the most prevalent cause of bacterial diarrhea worldwide. despite the serious health problems caused by this bacterium, human innate immune responses to c. jejuni infection remain poorly defined. human beta-defensins, a family of epithelial antimicrobial peptides, are a major component of host innate defense at the gastrointestinal mucosal surface. in this study, the effect of two different c. jejuni wild-type strains on human intestinal epithelial innate responses was in ... | 2005 | 16239524 |
| campylobacter susceptibility to ciprofloxacin and corresponding fluoroquinolone concentrations within the gastrointestinal tracts of chickens. | this study evaluated the relationship between campylobacter susceptibility and enteric fluoroquinolone concentrations in chickens treated with different doses of enrofloxacin. | 2005 | 16238734 |
| the heterogeneity of campylobacter jejuni and campylobacter coli strains isolated from healthy cattle. | to identify campylobacters isolated from clinically healthy cattle at species level by a multiplex polymerase chain reaction (m-pcr). the heterogeneity among campylobacter jejuni and campylobacter coli isolates was also investigated by using a restriction fragment length polymorphism (rflp) analysis of flagellin (flaa) gene. | 2005 | 16238642 |
| bile salts modulate expression of the cmeabc multidrug efflux pump in campylobacter jejuni. | cmeabc, a multidrug efflux pump, is involved in the resistance of campylobacter jejuni to a broad spectrum of antimicrobial agents and is essential for campylobacter colonization in animal intestine by mediating bile resistance. previously, we have shown that expression of this efflux pump is under the control of a transcriptional repressor named cmer. inactivation of cmer or mutation in the cmeabc promoter (pcmeabc) region derepresses cmeabc, leading to overexpression of this efflux pump. howev ... | 2005 | 16237025 |
| comparative phylogenomics of the food-borne pathogen campylobacter jejuni reveals genetic markers predictive of infection source. | campylobacter jejuni is the predominant cause of bacterial gastroenteritis worldwide, but traditional typing methods are unable to discriminate strains from different sources that cause disease in humans. we report the use of genomotyping (whole-genome comparisons of microbes using dna microarrays) combined with bayesian-based algorithms to model the phylogeny of this major food-borne pathogen. in this study 111 c. jejuni strains were examined by genomotyping isolates from humans with a spectrum ... | 2005 | 16230626 |
| carbohydrate mimicry: a new paradigm of autoimmune diseases. | molecular mimicry of microbial components by self components is thought to be the mechanism that accounts for the antigen and tissue specificity of immune responses in post-infectious autoimmune diseases. little direct evidence exists, and research in this area has focused principally on t cell mediated anti-peptide responses, rather than on humoral responses to carbohydrate structures. guillain-barré syndrome, the most frequent cause of acute neuromuscular paralysis, sometimes occurs after camp ... | 2005 | 16229995 |
| solid phase cytometry as a tool to detect viable but non-culturable cells of campylobacter jejuni. | solid phase cytometry (spc) in conjunction with fluorescent viability staining has been investigated as a tool to detect viable but non-culturable campylobacter jejuni in drinking water. inoculated water samples were filtered over a polyester membrane filter and the retained cells were stained using a carboxyfluorescein ester as a substrate for intracellular esterases. the number of green fluorescent bacteria was automatically counted by an ar laser scanning device (chemscan) in 3 min. in parall ... | 2005 | 16226638 |
| differentiation of regions with atypical oligonucleotide composition in bacterial genomes. | complete sequencing of bacterial genomes has become a common technique of present day microbiology. thereafter, data mining in the complete sequence is an essential step. new in silico methods are needed that rapidly identify the major features of genome organization and facilitate the prediction of the functional class of orfs. we tested the usefulness of local oligonucleotide usage (ou) patterns to recognize and differentiate types of atypical oligonucleotide composition in dna sequences of ba ... | 2005 | 16225667 |
| in vitro activities of 11 fluoroquinolones against 226 campylobacter jejuni strains isolated from finnish patients, with special reference to ciprofloxacin resistance. | although campylobacter jejuni is naturally susceptible to fluoroquinolones, the resistance to these antimicrobials has increased rapidly during the recent years. the aim of this study was to compare the activities of various older and newer fluoroquinolones towards c. jejuni, with special attention on ciprofloxacin-resistant strains. | 2005 | 16223936 |
| mechanism of diarrhea in microscopic colitis. | to search the pathophysiological mechanism of diarrhea based on daily stool weights, fecal electrolytes, osmotic gap and ph. | 2005 | 16222750 |
| [specified pathogen-free poultry flocks: the current situation]. | before the implementation of strategies to establish specified pathogen-free commercial poultry flocks, the ultimate goals need to be identified: 1) consumer protection by minimizing the risk for zoonotic diseases and food-borne pathogens, and/or 2) animal health protection against primary and secondary pathogens. the success for the establishment of specific pathogen-free poultry flocks depends on the biological characteristics, the epidemiological distribution and the transmission route of eac ... | 2005 | 16218186 |
| co-infection with two different campylobacter jejuni strains in a patient with the guillain-barré syndrome. | campylobacter jejuni is the predominant cause of antecedent infection in guillain-barré syndrome (gbs) or miller fisher syndrome (mfs). c. jejuni probably triggers gbs or mfs through molecular mimicry between bacterial sialylated lipo-oligosaccharides (los) and gangliosides in peripheral nerve tissue. we investigated whether co-infections with multiple c. jejuni strains occur in gbs or mfs patients and we further characterized these strains. pfge analysis of 83 c. jejuni isolates from single pri ... | 2006 | 16213180 |
| delineation of campylobacter concisus genomospecies by amplified fragment length polymorphism analysis and correlation of results with clinical data. | campylobacter concisus has been as frequently isolated from human diarrhea as the important enteropathogen campylobacter jejuni, but it also occurs in the feces of healthy individuals. the role of c. concisus in human disease has been difficult to determine, since the species comprises at least two phenotypically indistinguishable but genetically distinct taxa (i.e., genomospecies) that may vary in pathogenicity. we examined 62 c. concisus strains by amplified fragment length polymorphism (aflp) ... | 2005 | 16207968 |
| [antibodies to ganglioside gm1 and campylobacter jejuni in patients with guillain-barré syndrome]. | guillain-barré syndrome (gbs) is an acute immune mediated neuropathy, polyradiculoneuritis, characterized by rapid onset of symmetric extremity muscle paralysis, areflexia and albuminocytological dissociation in the cerebrospinal fluid (csf). recently, the heterogeneity of gbs has been noticed with definition of several gbs variants. the axonal gbs associated with anti-gm1 antibodies is the most important variant with the specific role of campylobacterjejuni (cj) in the induction of the disease. ... | 2005 | 16206698 |
| differentiation of campylobacter populations as demonstrated by flagellin short variable region sequences. | the dna sequence of the flaa short variable region (svr) was used to analyze a random population of campylobacter isolates to investigate the weakly clonal population structure of members of the genus. the svr sequence from 197 strains of c. jejuni and c. coli isolated from humans, bovine, swine, and chickens identified a group of 43 strains containing disparate short variable region sequences compared to the rest of the population. this group contains both c. jejuni and c. coli strains but disp ... | 2005 | 16204559 |
| speciation of campylobacter coli, c. jejuni, c. helveticus, c. lari, c. sputorum, and c. upsaliensis by matrix-assisted laser desorption ionization-time of flight mass spectrometry. | multiple strains of campylobacter coli, c. jejuni, c. helveticus, c. lari, c. sputorum, and c. upsaliensis isolated from animal, clinical, or food samples have been analyzed by matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms). whole bacterial cells were harvested from colonies or confluent growth on agar and transferred directly into solvent and then to a spot of dried 3-methoxy-4-hydroxycinnamic acid (matrix). multiple ions in the 5,000- to 15,000-da m ... | 2005 | 16204551 |
| genomic diversity of campylobacter coli and campylobacter jejuni isolates recovered from free-range broiler farms and comparison with isolates of various origins. | in many industrialized countries, the incidence of campylobacteriosis exceeds that of salmonellosis. campylobacter bacteria are transmitted to humans mainly in food, especially poultry meat products. total prevention of campylobacter colonization in broiler flocks is the best way to reduce (or eliminate) the contamination of poultry products. the aim of this study was to establish the sources and routes of contamination of broilers at the farm level. molecular typing methods (dna macrorestrictio ... | 2005 | 16204541 |
| effects of orally administered tetracycline on the intestinal community structure of chickens and on tet determinant carriage by commensal bacteria and campylobacter jejuni. | there is a growing concern that antibiotic usage in animal production has selected for resistant food-borne bacteria. since tetracyclines are common therapeutic antibiotics used in poultry production, we sought to evaluate the effects of oral administration on the resistance of poultry commensal bacteria and the intestinal bacterial community structure. the diversity indices calculated from terminal restriction fragment length polymorphism (t-rflp) analysis of 16s rrna amplicons did not indicate ... | 2005 | 16204498 |
| quantifying transmission of campylobacter spp. among broilers. | campylobacter species are frequently identified as a cause of human gastroenteritis, often from eating or mishandling contaminated poultry products. quantitative knowledge of transmission of campylobacter in broiler flocks is necessary, as this may help to determine the moment of introduction of campylobacter in broiler flocks more precisely. the aim of this study was to determine the transmission rate parameter in broiler flocks. four experiments were performed, each with four campylobacter-ino ... | 2005 | 16204486 |
| quantification of campylobacter spp. in chicken rinse samples by using flotation prior to real-time pcr. | real-time pcr is fast, sensitive, specific, and can deliver quantitative data; however, two disadvantages are that this technology is sensitive to inhibition by food and that it does not distinguish between dna originating from viable, viable nonculturable (vnc), and dead cells. for this reason, real-time pcr has been combined with a novel discontinuous buoyant density gradient method, called flotation, in order to allow detection of only viable and vnc cells of thermotolerant campylobacters in ... | 2005 | 16204485 |
| increasing prevalence of campylobacter jejuni in feedlot cattle through the feeding period. | the prevalence of campylobacter jejuni in commercial feedlot cattle was monitored throughout the feeding period by repeated bacteriologic culture of feces. fecal pats (n = 10) in 20 feedlot pens were sampled at 2-weeks interval beginning at entry into the feedlot and continuing until slaughter. the least-squares mean c. jejuni prevalence increased from 1.6% at the first sampling to 61.3% at the final sampling just prior to slaughter. diverse c. jejuni pulsed-field gel electrophoresis macrorestri ... | 2005 | 16204484 |
| investigating bacterial n-linked glycosylation: synthesis and glycosyl acceptor activity of the undecaprenyl pyrophosphate-linked bacillosamine. | the chemical synthesis and biological activity of undecaprenyl pyrophosphate bacillosamine (und-pp-bac), an obligatory intermediate in the asparagine-linked glycosylation pathway of campylobacter jejuni, are reported. the key transformation involves the coupling of bacillosamine phosphate and undecaprenyl phosphate. the synthetic und-pp-bac can be used to investigate the activity of the enzyme pgla, which catalyzes the first glycosyl transfer in substrate biosynthesis for n-linked protein glycos ... | 2005 | 16201778 |
| in search of human-associated bacterial pathogens in antarctic wildlife: report from six penguin colonies regularly visited by tourists. | we investigated the potential role of antarctic tourism in the introduction of human-associated pathogens into antarctic wildlife. we collected and analyzed 233 fecal samples from eight bird species. the samples were collected at six localities on the antarctic peninsula, which often is visited by tourists. every sample was investigated for pathogens of potential human origin: campylobacter jejuni, salmonella spp., and yersina spp. none of these bacteria was found. our data suggest that the tour ... | 2005 | 16201212 |
| misfolding of a bacterial autotransporter. | the adhesin involved in diffuse adherence (aida) is an autotransporter protein that confers the diffuse adherence phenotype to certain diarrheagenic escherichia coli strains. it consists of a 49 amino acid signal peptide, a 797 amino acid passenger domain, and a 440 amino acid beta-domain integrated into the outer membrane. the beta-domain consists of two parts: the beta(1)-domain, which is predicted to form two beta-strands on the bacterial cell surface, and the beta(2)-domain, which constitute ... | 2005 | 16199663 |
| the players in a mutualistic symbiosis: insects, bacteria, viruses, and virulence genes. | aphids maintain mutualistic symbioses involving consortia of coinherited organisms. all possess a primary endosymbiont, buchnera, which compensates for dietary deficiencies; many also contain secondary symbionts, such as hamiltonella defensa, which confers defense against natural enemies. genome sequences of uncultivable secondary symbionts have been refractory to analysis due to the difficulties of isolating adequate dna samples. by amplifying dna from hemolymph of infected pea aphids, we obtai ... | 2005 | 16195380 |
| role of dpra in transformation of campylobacter jejuni. | the role of a dpra ortholog (cj0634) in campylobacter jejuni transformation was phenotypically assessed using two strains. c. jejuni strain 11168 was naturally competent for transformation by chromosomal dna, while efficiency decreased 100-fold in a cj0634::apha mutant, whereas c. jejuni strain 480 was not naturally competent. c. jejuni strain 480 but not 11168 could be electro-transformed by shuttle plasmid pry111, an effect completely abolished by cj0634 interruption. complementation of the cj ... | 2005 | 16194595 |
| multiplex pcr for identification of campylobacter coli and campylobacter jejuni from pure cultures and directly on stool samples. | a multiplex-pcr method, specifically designed for application in routine diagnostic laboratories, was developed for the detection of campylobacter coli and campylobacter jejuni. primers were directed towards the following loci: the hippuricase gene (hipo) characteristic of c. jejuni, a sequence partly covering an aspartokinase gene characteristic of c. coli, and a universal 16s rdna gene sequence serving as an internal positive control for the pcr. the method was tested on 47 c. coli strains and ... | 2005 | 16192435 |
| [immunopathological evidence of terminal residues containing sialic acid in campylobacter jejuni lipopolysaccharide as the critical antigen to induce peripheral neuropathy]. | to explore the important role of the terminal residues containing sialic acid (sa) in campylobacter jejuni (cj) lipopolysaccharide (lps) as the critical antigen to induce nerve damage, and also to identify immunopathological evidence for the hypothesis of molecular mimicry and cross-immunity between cj lps and gangliosides. | 2005 | 16191299 |
| mathematical design of prokaryotic clone-based microarrays. | clone-based microarrays, on which each spot represents a random genomic fragment, are a good alternative to open reading frame-based microarrays, especially for microorganisms for which the complete genome sequence is not available. since the generation of a genomic dna library is a random process, it is beforehand uncertain which genes are represented. nevertheless, the genome coverage of such an array, which depends on different variables like the insert size and the number of clones in the li ... | 2005 | 16191193 |
| in vitro assembly of the undecaprenylpyrophosphate-linked heptasaccharide for prokaryotic n-linked glycosylation. | campylobacter jejuni has a general n-linked glycosylation pathway (encoded by the pgl gene cluster), which culminates in the transfer of a heptasaccharide: galnac-alpha1,4-galnac-alpha1,4-(glcbeta1,3)-galnac-alpha1,4-galnac-alpha1,4-galnac-alpha1,3-bac [where bac is bacillosamine (2,4-diacetamido-2,4,6-trideoxyglucose)] from a membrane-anchored undecaprenylpyrophosphate (und-pp)-linked donor to the asparagine side chain of proteins at the asn-x-ser/thr motif. herein we report, the cloning, overe ... | 2005 | 16186480 |
| [spontaneous bacterial empyema due to campylobacter jejuni]. | 2005 | 16185586 | |
| [pcr-rflp for campylobacter jejuni subtyping]. | ten campylobacter jejuni isolates, 8 identified as c. jejuni biotype ii of lior and 2 as c. jejuni biotipe i, were recovered from aborted pig fetuses. in order to discriminate among strains, restriction fragment length polymorphism (rflp) using ddel of polymerase chain reaction (pcr) products of flaa gen was used. c. jejuni biotype ii strains could be diferenciated in 6 by pcr-rflp, and one subtype was obtained from c. jejunibiotype i. although there is great variability of molecular techniques ... | 2005 | 16178461 |
| a computational method to predict genetically encoded rare amino acids in proteins. | in several natural settings, the standard genetic code is expanded to incorporate two additional amino acids with distinct functionality, selenocysteine and pyrrolysine. these rare amino acids can be overlooked inadvertently, however, as they arise by recoding at certain stop codons. we report a method for such recoding prediction from genomic data, using read-through similarity evaluation. a survey across a set of microbial genomes identifies almost all the known cases as well as a number of no ... | 2005 | 16168086 |
| three cases of cardiac complications associated with campylobacter jejuni infection and review of the literature. | presented here are three cases of acute cardiac disease (myocarditis, myopericarditis, and acute atrial fibrillation) associated with campylobacter jejuni infection, followed by a review of the corresponding literature. since campylobacter jejuni is the most common cause of human bacterial enteritis in developed countries, these cases emphasize the importance of keeping cardiac complications in mind when treating patients with acute gastroenteritis due to this pathogen. | 2005 | 16167138 |
| ocular adnexal lymphoma: a clinicopathologic study of a large cohort of patients with no evidence for an association with chlamydia psittaci. | non-hodgkin lymphomas are among the most common primary tumors occurring in the ocular adnexa. herein, we present a 14-year single-institution experience in 62 patients with primary ocular adnexal lymphomas (oals). association with chlamydia psittaci infection is examined in 57 tumor specimens. extranodal marginal zone lymphoma (emzl) was the most frequent histologic subtype (89%). the majority of patients with emzl (84%) presented with stage e-extranodal (ie), however only 16% had an advanced s ... | 2006 | 16166588 |
| campylobacter gene polymorphism as a determinant of clinical features of guillain-barré syndrome. | ganglioside epitopes on campylobacter jejuni are hypothesized as the key to the development and characterization of guillain-barré syndrome (gbs), but a comprehensive theory has yet to be established. a c jejuni gene, cst-ii, involved in the biosynthesis of ganglioside-like lipo-oligosaccharide, shows a polymorphism (asn/thr51) that affects ganglioside epitopes. | 2005 | 16162859 |
| mortality following campylobacter infection: a registry-based linkage study. | campylobacteriosis is one of the most commonly identified causes of bacterial diarrheal disease and a common cause of gastroenteritis in travellers from developed nations. despite the widespread occurrence, there is little information on campylobacter mortality. | 2005 | 16162289 |
| laterally transferred elements and high pressure adaptation in photobacterium profundum strains. | oceans cover approximately 70% of the earth's surface with an average depth of 3800 m and a pressure of 38 mpa, thus a large part of the biosphere is occupied by high pressure environments. piezophilic (pressure-loving) organisms are adapted to deep-sea life and grow optimally at pressures higher than 0.1 mpa. to better understand high pressure adaptation from a genomic point of view three different photobacterium profundum strains were compared. using the sequenced piezophile p. profundum strai ... | 2005 | 16162277 |
| factors affecting the pressure resistance of some campylobacter species. | to compare pressure resistance between strains of campylobacter jejuni, campylobacter coli, campylobacter lari and campylobacter fetus, and to investigate the effect of suspending medium on pressure resistance of sensitive and more resistant strains. | 2005 | 16162138 |
| campylobacter jejuni. | this review describes characteristics of the family campylobacteraceae and traits of campylobacter jejuni. the review then focuses on the worldwide problem of c. jejuni antimicrobial resistance and mechanisms of pathogenesis and virulence. unravelling these areas will help with the development of new therapeutic agents and ultimately decrease illness caused by this important human pathogen. | 2005 | 16162134 |
| roles of the regulatory proteins flhf and flhg in the vibrio cholerae flagellar transcription hierarchy. | vibrio cholerae, the causative agent of the human diarrheal disease cholera, is a motile bacterium with a single polar flagellum, and motility has been inferred to be an important aspect of virulence. the v. cholerae flagellar hierarchy is organized into four classes of genes. the expression of each class of genes within a flagellar hierarchy is generally tightly regulated in other bacteria by both positive and negative regulatory elements. to further elucidate flagellar biogenesis in v. cholera ... | 2005 | 16159765 |
| real-time single-nucleotide polymorphism profiling using taqman technology for rapid recognition of campylobacter jejuni clonal complexes. | the rapid identification of campylobacter jejuni isolates to strain level would significantly inform the public health investigation of c. jejuni infection. conceptual advances provided by multilocus sequence typing (mlst) have established the clonal complex as an important epidemiological group at the strain level, enabling accurate and phylogenetically valid strain identification for c. jejuni. the development of real-time pcr assays for allelic discrimination of strain-associated single-nucle ... | 2005 | 16157544 |
| recent developments in miller fisher syndrome and related disorders. | miller fisher syndrome is a localized variant of guillain-barré syndrome, characterized by ophthalmoplegia, areflexia and ataxia. bickerstaff's brainstem encephalitis is a related syndrome in which upper motor neurone features accompany the classic triad. anti-gq1b antibodies are uniquely found in both conditions and are believed to be pathogenic. | 2005 | 16155441 |
| ganglioside mimicry as a cause of guillain-barré syndrome. | campylobacter jejuni is the most frequent agent of antecedent infection in an axonal variant of guillain-barré syndrome, acute motor axonal neuropathy, and anti-gm1 or anti-gd1a igg antibody is also associated with acute motor axonal neuropathy. molecular mimicry has been found between human gm1 ganglioside and the lipo-oligosaccharide of c. jejuni isolated from an acute motor axonal neuropathy patient. progress has been made in guillain-barré syndrome research, especially on acute motor axonal ... | 2005 | 16155440 |
| biogenesis, architecture, and function of bacterial type iv secretion systems. | type iv secretion (t4s) systems are ancestrally related to bacterial conjugation machines. these systems assemble as a translocation channel, and often also as a surface filament or protein adhesin, at the envelopes of gram-negative and gram-positive bacteria. these organelles mediate the transfer of dna and protein substrates to phylogenetically diverse prokaryotic and eukaryotic target cells. many basic features of t4s are known, including structures of machine subunits, steps of machine assem ... | 2005 | 16153176 |
| ca2+ release from host intracellular stores and related signal transduction during campylobacter jejuni 81-176 internalization into human intestinal cells. | campylobacter jejuni is the leading bacterial cause of human diarrhoeal disease in many parts of the world, including the usa. the ability of c. jejuni to invade the host intestinal epithelium is an important determinant of virulence. a common theme among pathogenic invasive micro-organisms is their ability to usurp the eukaryotic cell-signalling systems both to allow for invasion and to trigger disease pathogenesis. ca(2+) is very important in a great variety of eukaryotic cell-signalling proce ... | 2005 | 16151220 |
| survival of campylobacter jejuni in waterborne protozoa. | the failure to reduce the campylobacter contamination of intensively reared poultry may be partially due to campylobacter resisting disinfection in water after their internalization by waterborne protozoa. campylobacter jejuni and a variety of waterborne protozoa, including ciliates, flagellates, and alveolates, were detected in the drinking water of intensively reared poultry by a combination of culture and molecular techniques. an in vitro assay showed that c. jejuni remained viable when inter ... | 2005 | 16151149 |
| new gammaproteobacteria associated with blood-feeding leeches and a broad phylogenetic analysis of leech endosymbionts. | many monophagous animals have coevolutionary relationships with bacteria that provide unavailable nutrients to the host. frequently, these microbial partners are vertically inherited and reside in specialized structures or tissues. here we report three new lineages of bacterial symbionts of blood-feeding leeches, one from the giant amazonian leech, haementeria ghilianii, and two others from placobdelloides species. these hosts each possess a different mycetome or esophageal organ morphology wher ... | 2005 | 16151107 |
| colonization of cattle intestines by campylobacter jejuni and campylobacter lanienae. | the location and abundance of campylobacter jejuni and campylobacter lanienae in the intestines of beef cattle were investigated using real-time quantitative pcr in two studies. in an initial study, digesta and tissue samples were obtained along the digestive tract of two beef steers known to shed c. jejuni and c. lanienae (steers a and b). at the time of slaughter, steer b weighed 540 kg, compared to 600 kg for steer a, yet the intestine of steer b (40.5 m) was 36% longer than the intestine of ... | 2005 | 16151098 |
| survival of campylobacter jejuni in water: effect of grazing by the freshwater crustacean daphnia carinata (cladocera). | environmental studies of the human-pathogenic bacterium campylobacter jejuni have focused on linking distributions with potential sources. however, in aquatic ecosystems, the abundance of c. jejuni may also be regulated by predation. we examine the potential for grazing by the freshwater planktonic crustacean daphnia carinata to reduce the survival of c. jejuni. we use a system for measuring grazing and clearance rates of d. carinata on bacteria and demonstrate that d. carinata can graze c. jeju ... | 2005 | 16151090 |