Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| giant cell arteritis, polymyalgia rheumatica, and viral hypotheses: a multicenter, prospective case-control study. groupe de recherche sur l'artérite à cellules géantes. | although suspected, a viral etiology has never been proven in giant cell arteritis (gca). we tested for viruses known to induce multinucleated giant cells in human pathology, which include the parainfluenza viruses (hpiv), respiratory syncytial virus, measles virus, herpesviruses type 1 and 2, and the epstein-barr virus. | 1999 | 9972970 |
| beta-subunit of nuclear pore-targeting complex (importin-beta) can be exported from the nucleus in a ran-independent manner. | the nuclear export of importin-alpha is mediated by cas, which is related to importin-beta, whereas the mechanism for the export of importin-beta remains unclear. in this study, we demonstrate that the nuclear export of importin-beta is mediated by the nuclear pore complex-binding domain of this molecule. insensitivity to leptomycin b indicates that its export is not mediated by a leucine-rich nuclear export signal-specific receptor, crm1. furthermore, the nuclear export of importin-beta was not ... | 1999 | 9933584 |
| gene transfer of human prostacyclin synthase prevents neointimal formation after carotid balloon injury in rats. | a disordered proliferative process in the vascular wall is thought to underlie the pathogenesis of restenosis after percutaneous transluminal angioplasty and carotid endarterectomy. a growth inhibitory property of overexpressed prostacyclin (pgi2) synthase (pgis) was recently implicated in the pathological proliferation of vascular smooth muscle cells (vsmc) in vitro. here, we investigated the effects of increased pgi2 synthesis on the pathological proliferation of vsmcs. | 1999 | 9933282 |
| gene transfer in utero biologically engineers a patent ductus arteriosus in lambs by arresting fibronectin-dependent neointimal formation. | closure of the ductus arteriosus requires prenatal formation of intimal cushions, which occlude the vessel lumen at birth. survival of newborns with severe congenital heart defects, however, depends on ductal patency. we used a gene transfer approach to create a patent ductus arteriosus by targeting the fibronectin-dependent smooth muscle cell migration required for intimal cushion formation. fetal lamb ductus arteriosus was transfected in utero with hemagglutinating virus of japan liposomes con ... | 1999 | 9930865 |
| early biological effect of in vivo gene transfer of platelet-derived growth factor (pdgf)-b into healing patellar ligament. | to define the early biological effect of in vivo introduction of the pdgf-b gene on the healing of ligaments, a hvj-liposome suspension containing platelet-derived growth factor (pdgf)-b cdna was injected directly into the injured patellar ligament of 14-week-old male wistar rats. rats were killed at 1, 4 and 8 weeks for the morphological analysis of angiogenesis by laminin immunohistochemistry and of collagen deposition by masson's trichrome staining and collagen i immunohistochemistry. pdgf-b ... | 1998 | 9930316 |
| efficient transfer of oligonucleotides and plasmid dna into the whole heart through the coronary artery. | several of the current techniques for transfer of both oligonucleotide and plasmid dna into the myocardium are impaired by low efficiency and toxicity. to improve gene transfer techniques, especially into the whole heart, a gene transfer method involving liposome in conjunction with a viral envelope (hvj-liposome) was essayed as an alternative. fitc-labeled oligonucleotide (f-odn) and the cdna of beta-galactosidase (beta-gal) were introduced into the myocardium by coronary infusion of hvj-liposo ... | 1998 | 9930300 |
| a comparison of in vivo gene delivery methods for antisense therapy in ligament healing. | to determine the most efficient in vivo delivery method of oligonucleotides for antisense therapy in ligament healing, fluorescence-labelled phosphorothioate oligodeoxynuleotides (odn) were introduced into 12 rabbit ligament scars 2 weeks after injury using haemagglutinating virus of japan (sendai virus: hvj)-conjugated liposomes. we compared the efficiency of cellular uptake of fluorescence as a percentage of all cells in each scar using three delivery procedures: (1) direct free-hand injection ... | 1998 | 9930298 |
| determinants of pantropism of the f1-r mutant of sendai virus: specific mutations involved are in the f and m genes. | mutations in the fusion, f, protein of sendai virus resulting in increased cleavability by ubiquitous host protease(s), and mutations in the matrix, m, protein resulting in bipolar budding, are both important determinants for the systemic infection in mice caused by the protease activating pantropic mutant, f1-r. several mutants of sendai virus (by, bf, and kd-m) with phenotypes of bipolar budding and/or increased cleavability of f protein were isolated. genomic rna sequence analysis of the f an ... | 1998 | 9930191 |
| paramyxovirus fusion protein: characterization of the core trimer, a rod-shaped complex with helices in anti-parallel orientation. | the fusion (f) protein of the paramyxovirus sv5 contains two heptad repeat regions, hra adjacent to the fusion peptide and hrb proximal to the transmembrane domain. peptides, n-1 and c-1, respectively, corresponding to these heptad repeat regions form a thermostable, alpha-helical trimer of heterodimers (s. b. joshi, r. e. dutch, and r. a. lamb (1998). virology 248, 20-34). further characterization of the n-1/c-1 complex indicated that the c-1 peptides, which are predicted to residue on the outs ... | 1999 | 9927582 |
| prevalence of various respiratory viruses in the middle ear during acute otitis media. | vaccines against respiratory viruses may be able to reduce the frequency of acute otitis media. although the role of respiratory viruses in the pathogenesis of acute otitis media is well established, the relative importance of various viruses is unknown. | 1999 | 9920949 |
| essential role of interferon regulatory factor 3 in direct activation of rantes chemokine transcription. | localized and systemic cytokine production in virus-infected cells play an important role in the outcome of viral infection and pathogenicity. activation of the interferon regulatory factors (irf) in turn is a critical mediator of cytokine gene transcription. recent studies have focused on the 55-kda irf-3 gene product as a direct transcriptional regulator of type 1 interferon (ifn-alpha and ifn-beta) activation in response to virus infection. virus infection induces phosphorylation of irf-3 on ... | 1999 | 9891032 |
| target-cell specificity of fusogenic liposomes: membrane fusion-mediated macromolecule delivery into human blood mononuclear cells. | fusogenic liposome, a unique vector prepared by fusing ultraviolet-inactivated sendai virus and liposome, is known to efficiently deliver content into various animal cells through membrane fusion. in this study, we examined the target-cell specificity of fusogenic liposome (fl)-mediated macromolecule delivery into human blood cells using diphtheria toxin fragment a (dta) as a probe. among the peripheral blood mononuclear cells (pbmc), fl was able to deliver its encapsulates into cd14+ monocytes ... | 1999 | 9889393 |
| compatibility of plasmids expressing different antigens in a single dna vaccine formulation. | one anticipated advantage of dna immunization is the potential to create multivalent vaccines. we have examined the effects of mixing plasmids into single formulations using plasmids expressing four different membrane bound glycoproteins from bovine herpesvirus-1 (bhv-1), bovine parainfluenzavirus-3 (bpi3) and human influenza virus (hinf). plasmids were delivered by intradermal injection into the tails of mice and the types of responses generated were clearly affected by the expressed antigen. p ... | 1998 | 9880010 |
| membrane-induced step in the activation of sendai virus fusion protein. | peptides derived from conserved heptad-repeat regions of several viruses have been shown recently to inhibit virus-cell fusion. to find out their possible role in the fusion process, two biologically active heptad-repeat segments of the fusion protein (f) of sendai virus, sv-150 (residues 150-186), and sv-473 (residues 473-495) were synthesized, fluorescently labeled and spectroscopically characterized for their structure and organization in solution and within the membrane. sv-150 was found to ... | 1999 | 9878433 |
| isolation of avian serotype 3 paramyxoviruses from imported caged birds in israel. | ten avian serotype 3 paramyxoviruses were isolated for the first time in israel from passerine and psittacine imported caged birds. the birds were submitted for investigation of an illness characterized by nonspecific signs of weakness, anorexia, vomiting, and sneezing. in addition, only the parakeets developed specific neurologic signs. in bacteriologic and pathologic investigation, cachexia and diarrhea were observed in both groups of birds. in psittacines, considerable alterations were observ ... | 1998 | 9876858 |
| hvj-liposome mediated gene transfer into hepatocytes in vivo. | the efficient transduction of appropriate target cells will be critical for gene therapy. we evaluated the suitability of hemagglutinating virus of japan (hvj)-liposome-mediated gene transfer for gene therapy of liver diseases. | 1998 | 9875637 |
| a potent and selective inhibition of parainfluenza 1 (sendai) virus by new 6-oxiranyl-, 6-methyloxiranyluracils, and 4(3h)-pyrimidinone derivatives. | several new 6-oxiranyl-, 6-methyloxiranyluracils, and pyrimidinone derivatives, synthesized by the lithiation-alkylation sequence of 1,3,6-trimethyluracil, 1,3-dimethyl-6-chloromethyluracil, and 2-alkoxy-6-methyl-4(3h)-pyrimidinones, showed a potent and selective antiviral activity against the parainfluenza 1(sendai) virus replication. | 1998 | 9873443 |
| ip-10 gene transcription by virus in astrocytes requires cooperation of isre with adjacent kappab site but not irf-1 or viral transcription. | transcription of the ip-10 gene requires interferon (ifn)-stimulated response element (isre) and kappab sites to be induced by lipopolysaccharide (lps), ifn-gamma, virus, and poly(i:c). a requirement for stat1 binding to isre for ifn-gamma and ifn regulatory factor-1 (irf-1) binding to isre for lps, poly(i:c), and virus has been reported. we investigated whether viral transcription is required for ip-10 induction and how isre interacts with irf-1 and with two kappab sites. ip-10 mrna was induced ... | 1998 | 9858321 |
| viruses and virus-like particles identified in ostrich gut contents. | samples of either gut content from ostriches showing symptoms of enteritis, or allantoic fluid of eggs inoculated with ostrich isolates, were examined for the presence of viral agents by direct negative-contrast electron microscopy. only a few virus types could be identified with certainty, namely a circovirus (1 sample), a coronavirus (1 sample), a member of either the toga- or bunyaviridae (1 sample), enterovirus (16 samples) and paramyxovirus (26 samples). a large number of samples contained ... | 1998 | 9850509 |
| rna replication for the paramyxovirus simian virus 5 requires an internal repeated (cgnnnn) sequence motif. | a functional rna replication promoter for the paramyxovirus simian virus 5 (sv5) requires two essential and discontinuous elements: 19 bases at the 3' terminus (conserved region i) and an 18-base internal region (conserved region ii [crii]) that is contained within the coding region of the l protein gene. a reverse-genetics system was used to determine the sequence requirements for the internal crii element to function in rna replication. a series of copyback defective interfering (di) rna analo ... | 1999 | 9847393 |
| sendai virus infection induces apoptosis through activation of caspase-8 (flice) and caspase-3 (cpp32). | sendai virus (sv) infection and replication lead to a strong cytopathic effect with subsequent death of host cells. we now show that sv infection triggers an apoptotic program in target cells. incubation of infected cells with the peptide inhibitor z-vad-fmk abrogated sv-induced apoptosis, indicating that proteases of the caspase family were involved. moreover, proteolytic activation of two distinct caspases, cpp32/caspase-3 and, as shown for the first time in virus-infected cells, flice/caspase ... | 1999 | 9847376 |
| two nucleotides immediately upstream of the essential a6g3 slippery sequence modulate the pattern of g insertions during sendai virus mrna editing. | editing of paramyxovirus p gene mrnas occurs cotranscriptionally and functions to fuse an alternate downstream open reading frame to the n-terminal half of the p protein. g residues are inserted into a short g run contained within a larger purine run (angn) in this process, by a mechanism whereby the transcribing polymerase stutters (i.e., reads the same template cytosine more than once). although sendai virus (sev) and bovine parainfluenza virus type 3 (bpiv3) are closely related, the g inserti ... | 1999 | 9847338 |
| the attachment protein of hendra virus has high structural similarity but limited primary sequence homology compared with viruses in the genus paramyxovirus. | the complete nucleotide sequence of the attachment protein gene of hendra virus, a new member of the subfamily paramyxovirinae, has been determined from cdna clones derived from viral genomic rna. the deduced mrna is 2565 nucleotides long with one open reading frame encoding a protein of 604 amino acids, which is similar in size to the attachment protein of the members of the subfamily. however, the mrna transcript is >600 nucleotides longer than others in the subfamily due to the presence of lo ... | 1998 | 9837786 |
| cellular effects induced by some synthetic prostaglandins obtained at iccf-bucharest. | four prostaglandin compounds synthetized at iccf [chloprostenol triacetate (a), the isopropylic ester of the chloprostenol analogue (b), a pga2 sulprostone analogue (c) and a pge2 analogue (d)] were tested with respect to the cytotoxic effect, by successive studies on chicken embryo fibroblasts (cef) and on human cells (hela). it was established that (b) and (d) display a high cytotoxicity, while (a) and (c) were relatively well tolerated by the cell layer. the spectrofluorometric determinations ... | 1997 | 9836326 |
| virological and pathomorphological aspects in experimental infections with some triassociated pneumotropic viruses in the white mouse. | experimental infections were induced in white mice by intranasal administration of parainfluenza virus type 3, 739-2d strain, to which influenza virus a/beijing, 353/89 (h3n2) strain, and respiratory syncytial virus, long strain, were associated. the model was organized so as to obtain a triassociated infection, parainfluenza virus type 3 being inoculated the first and the other two viruses, in the following stages-ii and iii-, alternately. the infections were revealed by the presence of positiv ... | 1997 | 9836325 |
| growth inhibition of cultured human tenon's fibroblastic cells by targeting the e2f transcription factor. | the transcription factor e2f regulates the expression of several genes concerned with cell growth. the ability to inhibit transcription by blocking e2f expression has great potential in the treatment of proliferative disorders. the effect of double-stranded phosphorothioate oligonucleotides containing e2f transcription factor cis element, a so called 'decoy' has examined on the growth of cultured human tenon's fibroblastic cells. human tenon's fibroblastic cells were cultured and challenged by e ... | 1998 | 9820786 |
| rapid and efficient recovery of sendai virus from cdna: factors influencing recombinant virus rescue. | in a comparative study the factors influencing the recovery of recombinant sendai viruses (sev) from plasmid based cdna were analysed systematically in order to establish an efficient and robust method for virus rescue. the amounts and ratios of transfected helper plasmids encoding the viral n, p and l proteins proved to be crucial for virus rescue, and they were optimised step-by-step for enhanced virus release. when the c open reading frame from the p gene was expressed at low level, virus res ... | 1998 | 9820574 |
| cytoplasmic domain of sendai virus hn protein contains a specific sequence required for its incorporation into virions. | in the assembly of paramyxoviruses, interactions between viral proteins are presumed to be specific. the focus of this study is to elucidate the protein-protein interactions during the final stage of viral assembly that result in the incorporation of the viral envelope proteins into virions. to this end, we examined the specificity of hn incorporation into progeny virions by transiently transfecting hn cdna genes into sendai virus (sv)-infected cells. sv hn expressed from cdna was efficiently in ... | 1998 | 9811709 |
| effects of anionic and nonionic polymers on fusion and binding of sendai virus to human erythrocyte ghosts. | effects of various polymers (dextran sulfate, dextran and polyethylene glycol) on binding and fusion of sendai virus to target cells were studied by use of fluorescence spectroscopy. direct binding of dextran sulfate but not dextran to sendai virus was detected. anionic and nonionic polymers showed definite effects on segmental motions of the viral envelope proteins. sendai virus binding to human erythrocyte ghost membranes (heg) was reduced by dextran sulfate and dextran while the fusion temper ... | 1998 | 9806488 |
| [a comparison of the nci-h292 line with other continuous lines for the multiplication of respiratory viruses]. | the nci-h292 continual line of mucoepidermoid cells of the human lungs has been reported to be useful for the propagation of many viruses, mainly adenovirus and paramyxovirus. it is stated the possible substitution of primary cultures of monkey kidney for nci-h292 in order to isolate such agents. in the present paper it is evaluated the utility of this line for multiplying the respiratory syncytial viruses adenovirus 3 and 7, and the parainfluenza viruses 1, 2, and 3, in comparison with the cont ... | 1996 | 9805045 |
| patients with systemic lupus erythematosus have reduced numbers of circulating natural interferon-alpha- producing cells. | systemic lupus erythematosus (sle) patients often have continuous production of interferon-alpha (ifn-alpha), but production of in vitro ifn-alpha by peripheral blood mononuclear cells (pbmc) may be varyingly reduced. we here report that ifn-alpha production induced by herpes simplex virus (hsv) in pbmc resembling immature dendritic cells and designated natural ifn-alpha producing cells (nipc), was much more affected than that induced by sendai virus (sv) in monocytes. at the cell level, the fre ... | 1998 | 9802930 |
| protective cytotoxic t lymphocyte responses against paramyxoviruses induced by epitope-based dna vaccines: involvement of ifn-gamma. | plasmid dna vectors have been constructed with minigenes encoding a single cytotoxic t lymphocyte (ctl) epitope from either the m2 protein of respiratory syncytial virus (rsv) or from the nucleoprotein of measles virus (mv) with or without a signal sequence (also called secretory or leader sequence). following intradermal immunization, plasmids in which the ctl epitopes were expressed in-frame with the signal sequence were more effective at inducing peptide- and virus-specific ctl responses than ... | 1998 | 9796910 |
| longitudinal analysis of the acute sendai virus-specific cd4+ t cell response and memory. | the development and persistence of sendai virus-specific cd4+ t cell memory has been analyzed following respiratory infection of c57bl/6j mice by determining the prevalence of il-2-producing th cell precursors (thp). frequencies as high as 1:40 virus-specific cd4+ t cells were found in the regional lymph nodes and spleen during the acute phase of the host response and persisted at levels > or =1:500 for 2 to 3 mo. thereafter, these cd4+ t cells tended to distribute more to the spleen than to the ... | 1998 | 9794378 |
| domain-structure of cytoplasmic border region is main determinant for palmitoylation of influenza virus hemagglutinin (h7). | we have shown previously that the length of cytoplasmic tails influences the selection of lipid substrates for palmitoylation of influenza viral hemagglutinin esterase fusion (hef) and hemagglutinin (ha) glycoproteins [veit et al. (1996) biochem. j. 318, 163-172; reverey et al. (1996) j. biol. chem. 271, 23607-23610]. using a series of new chimeric mutant proteins derived from acylated influenza virus ha (subtype h7) and from nonacylated sendai virus fusion protein (f, strain z), we report here ... | 1998 | 9791024 |
| [occurrence of paramyxovirus 1 (pmv 1) infections in pigeons]. | from 1992 to 1997 the occurrence of the paramyxovirus-1 infection in pigeons in the region of berlin-brandenburg was examined. using the haemagglutination inhibition test the presence of pmv 1/ndv could be demonstrated indirectly. there was a rather high percentage (19.9%) of pigeon sera containing specific antibodies. furthermore we isolated und classified pmv 1 from pigeons by standard methods, particular by intracerebral pathogenicity index (icpi) tests and binding studies using monoclonal an ... | 1998 | 9789370 |
| direct detection of respiratory syncytial virus, parainfluenza virus, and adenovirus in clinical respiratory specimens by a multiplex reverse transcription-pcr assay. | diagnosis of respiratory virus infections currently involves detection by isolation or antigen detection, which usually identifies only a single suspected agent. to permit identification of more than one respiratory virus in clinical specimens, a rapid detection method involving a single-step, multiplex reverse transcription-pcr (rt-pcr) assay was developed. the assay included five primer sets that amplified the rna of respiratory syncytial virus subtypes a and b, parainfluenza virus types 1, 2, ... | 1998 | 9774555 |
| the paramyxovirus sv5 small hydrophobic (sh) protein is not essential for virus growth in tissue culture cells. | the sh gene of the paramyxovirus sv5 is located between the genes for the glycoproteins, fusion protein (f) and hemagglutinin-neuraminidase (hn), and the sh gene encodes a small 44-residue hydrophobic integral membrane protein (sh). the sh protein is expressed in sv5-infected cells and is oriented in membranes with its n terminus in the cytoplasm. to study the function of the sh protein in the sv5 virus life cycle, the sh gene was deleted from the infectious cdna clone of the sv5 genome. by usin ... | 1998 | 9770417 |
| gene transfer to vein graft wall by hvj-liposome method: time course and localization of gene expression. | a novel gene transfer method using liposomes with a viral envelope of hemagglutinating virus of japan (hvj) has been reported to be very effective for gene transfection into somatic cells and might be applicable to improve the patency of vein grafts. the present study examined the time course and localization of gene expression to assess the feasibility of ex vivo gene transfer into the vein graft by the hvj-liposome method. | 1998 | 9768936 |
| structure-function study of a heptad repeat positioned near the transmembrane domain of sendai virus fusion protein which blocks virus-cell fusion. | a synthetic heptad repeat, sv-473, derived from sendai virus fusion protein is a potent inhibitor of virus-cell fusion. in order to understand the mechanism of the inhibitory effect, we synthesized and fluorescently labeled sv-465, an extended version of sv-473 by one more heptad, its mutant peptide a17,24-sv-465, in which two heptadic leucines were substituted with two alanines, and its enatiomer d-sv-465, composed entirely of damino acids. similar mutations in the homologous fusion protein of ... | 1998 | 9765238 |
| genetic characterization of parainfluenza virus 3 derived from guinea pigs. | to understand the relationship between novel parainfluenza virus 3 (piv-3), which has recently been isolated from the lungs of guinea pigs, and other piv-3 strains, we determined the complete nucleotide sequence of the novel piv-3 (gpv) genome. a comparison of the nucleotide sequence among piv-3s, including bovine piv-3, revealed that gpv is closely related to human piv-3. the results of the phylogenetic analysis clearly showed that gpv is a lineage of human piv-3, suggesting that gpv has probab ... | 1998 | 9764404 |
| immunocytochemical colocalization of specific immunoglobulin a with sendai virus protein in infected polarized epithelium. | immunoglobulin (ig)a provides the initial immune barrier to viruses at mucosal surfaces. specific iga interrupts viral replication in polarized epithelium during receptor-mediated transport, probably by binding to newly synthesized viral proteins. here, we demonstrate by immunoelectron microscopy that specific iga monoclonal antibodies (mabs) accumulate within sendai virus-infected polarized cell monolayers and colocalize with the hemagglutinin- neuraminidase (hn) viral protein in a novel intrac ... | 1998 | 9763601 |
| site-specific gene delivery in vivo through engineered sendai viral envelopes. | inspite of several stimulating developments in gene therapy, the formulation of a targeted gene delivery "vector" is still far from ideal. we have demonstrated the potential of reconstituted sendai viral envelopes containing only the fusion glycoprotein (f-virosomes) in targeted delivery of reporter genes to liver cells of balb/c mouse in vivo. the membrane fusion-mediated high efficiency of gene transfer to liver cells was ascertained following a critical evaluation of the level of the dna, mrn ... | 1998 | 9751760 |
| sendai virus fusion activity as modulated by target membrane components. | we have studied the differences between erythrocytes and erythrocyte ghosts as target membranes for the study of sendai virus fusion activity. fusion was monitored continuously by fluorescence dequenching of r 18-labeled virus. experiments were carried out either with or without virus/target membrane prebinding. when sendai virus was added directly to a erythrocyte/erythrocyte ghost suspension, fusion was always lower than that obtained when experiments were carried out with virus already bound ... | 1998 | 9743474 |
| gene transfer vectors based on sendai virus. | a gene delivery system is a fundamental technology used in human gene therapy. in order to treat patients suffering from incurable metabolic diseases, we must be able to deliver genes efficiently in situ and induce stable gene expression in non-dividing tissue cells. however, none of the current gene transfer systems (both viral and non-viral) satisfies this goal. in order to develop a novel gene delivery system that is free from the defects of existing gene transfer vectors, we analyzed natural ... | 1998 | 9741904 |
| fusion of sendai virus and individual host cells and inhibition of fusion by lipophosphoglycan measured with image correlation spectroscopy. | fusion between sendai virus (sv) and individual host cells was investigated with confocal laser scanning microscopy (clsm) and image correlation spectroscopy (ics). sv was labeled with the fluorescent probe 7-octadecylamino-4-nitrobenz-2-oxa-1,3-diazole (nbd-nh-c18) and was allowed to bind to host cells (hep-2, balb-3t3) at 4 degrees c. the effect of lipophosphoglycan (lpg), isolated from leishmania donovani, on virus fusion was investigated by incorporation of lpg (0, 5, 10 or 20 microm) into t ... | 1998 | 9739163 |
| outbreak of pneumonia in a long-term care facility: antecedent human parainfluenza virus 1 infection may predispose to bacterial pneumonia. | to determine the causes of an outbreak of lobar pneumonia. | 1998 | 9736104 |
| properties of lipoamino acids incorporated into membrane bilayers. | several lipoamino acids were synthesized in which palmitic acid was coupled with the alpha-amino group of an amino acid. these lipoamino acids were tested for their inhibitory action against sendai virus fusion to liposomes composed of egg phosphatidylethanolamine and 5 mol% of the ganglioside gd1a. a commonly employed viral fusion assay based on the dilution of the fluorescent probe octadecylrhodamine (r18) exhibited an additional complication in the presence of nalpha-palmitoyl tryptophan (pal ... | 1998 | 9733922 |
| membrane fusion promoted by increasing surface densities of the paramyxovirus f and hn proteins: comparison of fusion reactions mediated by simian virus 5 f, human parainfluenza virus type 3 f, and influenza virus ha. | the membrane fusion reaction promoted by the paramyxovirus simian virus 5 (sv5) and human parainfluenza virus type 3 (hpiv-3) fusion (f) proteins and hemagglutinin-neuraminidase (hn) proteins was characterized when the surface densities of f and hn were varied. using a quantitative content mixing assay, it was found that the extent of sv5 f-mediated fusion was dependent on the surface density of the sv5 f protein but independent of the density of sv5 hn protein, indicating that hn serves only a ... | 1998 | 9733810 |
| detection of mycoplasma pneumoniae in the airways of adults with chronic asthma. | infection with mycoplasma pneumoniae has been shown to exacerbate asthma in humans. however, the role of m. pneumoniae in the pathogenesis of chronic asthma has not been defined. eighteen asthmatics with chronic, stable asthma and 11 nonasthmatic control subjects underwent evaluation of the upper and lower airways and serologic analysis to determine the presence of m. pneumoniae, chlamydia pneumoniae, and seven respiratory viruses through culture, enzyme-linked immunoassay (eia) and polymerase c ... | 1998 | 9731038 |
| the mannose receptor mediates induction of ifn-alpha in peripheral blood dendritic cells by enveloped rna and dna viruses. | peripheral blood dendritic cells (dc) produce ifn-alpha in response to challenge by many enveloped viruses including herpes simplex virus (hsv) and hiv, whereas sendai virus predominantly stimulates ifn-alpha production by monocytes. glycosylated viral envelope proteins are known to be important for the induction of ifn-alpha. in this study we demonstrate that stimulation of ifn-alpha synthesis by hsv is inhibited by a number of monosaccharides, including fucose, n-acetylglucosamine, and n-acety ... | 1998 | 9725235 |
| binding of influenza and paramyxoviruses to group b streptococcus with the terminal sialyl-galactose linkage. | using the virus-binding assay and scanning electron microscopy (sem), influenza a and b type viruses and two paramyxoviruses, parainfluenza (sendai) and mumps viruses, were found to bind to group b streptococcus (gbs), type ia and ii, with the terminal sialyl-galactose linkage, although some viruses detached during the sample processing for sem, and mumps virus did not bind to gbsia. binding of viruses eluted from gbs at 37 degrees c depended on combination of virus and gbs. the biological signi ... | 1998 | 9723158 |
| myopericarditis associated with parainfluenza virus type 3 infection. | 1998 | 9721970 | |
| induction of neonatal th1 and ctl responses by live viral vaccines: a role for replication patterns within antigen presenting cells? | failure to generate ctl responses in early life has been linked to the preferential maturation of cd4 t cells into th2 rather than th1 cells in response to some, but not other, antigenic stimulations. here, we provide preliminary evidence for the role of the viral replication pattern in the shaping of neonatal cellular responses to live viral vaccines. neonatal and early life immunization with live attenuated sendai virus vaccine led to the induction of igg2a antibodies and cytotoxic responses a ... | 1998 | 9711791 |
| sendai virus y proteins are initiated by a ribosomal shunt. | the sendai virus p/c mrna expresses eight primary translation products by using a combination of ribosomal choice and cotranscriptional mrna editing. the longest open reading frame (orf) of the mrna starts at aug104 (the second initiation site) and encodes the 568-amino-acid p protein, an essential subunit of the viral polymerase. the first (acg81), third (atg114), fourth (atg183), and fifth (atg201) initiation sites are used to express a c-terminal nested set of polypeptides (collectively named ... | 1998 | 9710586 |
| molecular basis for naturally occurring elevated readthrough transcription across the m-f junction of the paramyxovirus sv5. | transcription of the paramyxovirus rna genome is thought to involve a sequential stop-start mechanism whereby monocistronic mrnas are produced by polyadenylation and termination of 3' upstream gene followed by reinitiation at the downstream start site. for a number of paramyxoviruses, transcription across the m-f gene junction results in the synthesis of high levels of a dicistronic m-f readthrough rna. in cells infected with the paramyxovirus sv5, 15% or less of the transcripts from the viral p ... | 1998 | 9705920 |
| a core trimer of the paramyxovirus fusion protein: parallels to influenza virus hemagglutinin and hiv-1 gp41. | the paramyxovirus fusion (f) protein mediates membrane fusion. the biologically active f protein consists of a membrane distal subunit, f2, and a membrane-anchored subunit, f1. we have identified a highly stable structure composed of peptides derived from the f1 heptad repeat a, which abuts the hydrophobic fusion peptide (peptide n-1), and the f1 heptad repeat b, located 270 residues downstream and adjacent to the transmembrane domain (peptides c-1 and c-2). in isolation, peptide n-1 is 47% alph ... | 1998 | 9705252 |
| antibody response of cattle experimentally inoculated with parainfluenza type-3 live vaccine in a post-tracheal position. | one of the major viral respiratory diseases of cattle is parainfluenza type-3 virus infection. attenuated live virus vaccines have been used in most countries. an experimental attempt was made to clarify the antibody response of cattle inoculated with a live vaccine in a post-tracheal position by a spray method. a significantly higher titers of antibodies in both hemagglutination inhibition and neutralization were obtained in the serum samples of animals vaccinated in this manner than those vacc ... | 1998 | 9699204 |
| the immunogenicity and efficacy of intranasally or parenterally administered replication-deficient vaccinia-parainfluenza virus type 3 recombinants in rhesus monkeys. | immunization of rhesus monkeys with modified vaccinia ankara (mva) recombinants expressing the haemagglutinin-neuraminidase (hn) or fusion (f) glycoproteins of human parainfluenza virus type 3 (hpiv3) was compared with an intranasally-administered live, attenuated hpiv3 vaccine candidate, the cp45 derivative of the js strain of wildtype hpiv3. the mva recombinants, when given parenterally (i.m.) or as a parenteral-local (i.m. and i.t.) combination, induced an antibody response comparable to that ... | 1998 | 9682397 |
| sequence analysis of the hendra virus nucleoprotein gene: comparison with other members of the subfamily paramyxovirinae. | the nucleoprotein (n) gene of hendra virus (hev), an unclassified member of subfamily paramyxovirinae in the family paramyxoviridae previously known as equine morbillivirus, was cloned and sequenced. the majority of the deduced amino acid sequence was further confirmed by direct sequencing of peptide fragments of the n protein derived from purified virions. the 3' untranslated sequence of the hev n gene mrna was 568 nt and was much longer than that observed in other paramyxovirinae. the n protei ... | 1998 | 9680142 |
| in vivo gene transfer methods in the bladder without viral vectors. | to examine three in vivo gene transfer methods, without viral vectors, for use in bladder cancer. | 1998 | 9666773 |
| molecular epidemiology of two consecutive outbreaks of parainfluenza 3 in a bone marrow transplant unit. | two consecutive nosocomial outbreaks of parainfluenza 3, in which 5 of 15 infected patients died, occurred in an adult bone marrow transplant unit. parainfluenza 3 strain variation was assessed by reverse transcription-pcr sequencing of part of the parainfluenza 3 f gene, including the noncoding region, directly from clinical samples. sequence data from the outbreaks were compared with those from 15 other parainfluenza 3 isolates circulating concurrently in the community; altogether, 13 strains ... | 1998 | 9666007 |
| paget's disease of bone. | paget's disease of bone is a localized disorder of bone remodeling. increased numbers of larger than normal osteoclasts initiate the process at affected skeletal sites, and the increase in bone resorption is followed by an increase in new bone formation, altering bone architecture. the signs and symptoms of paget's disease are varied, depending in part on the location of the involved sites and the degree of increased bone turnover. recent progress in paget's disease research includes new data re ... | 1998 | 9661069 |
| virus infection induces the assembly of coordinately activated transcription factors on the ifn-beta enhancer in vivo. | we have identified a virus-activated factor (vaf) that binds to a regulatory element shared by different virus-inducible genes. we provide evidence that vaf contains two members of the interferon regulatory factor (irf) family of transcriptional activator proteins (irf-3 and irf-7), as well as the transcriptional coactivator proteins p300 and cbp. remarkably, vaf, as well as recombinant irf-3 and irf-7 proteins, binds very weakly to the interferon-beta (ifn-beta) gene promoter in vitro. however, ... | 1998 | 9660935 |
| variations in the interferon-inducing capacity of sendai virus subpopulations. | several sendai virus (sv) preparations, propagated through eggs from the same viral seed, exhibited significantly different capacities to induce interferon (ifn) in human leukocytes (nhu-ifn-alpha). the amount of induced ifn and the numbers of sv ifn-inducing particles (ifp) per cell were determined in dose (sv concentration)-response (ifn yield) curves, kinetics of ifn production, and coinfection experiments with sv preparations that differed in ifn-inducing capacities. the possible role of leu ... | 1998 | 9660247 |
| structural characterisation of n-linked and o-linked oligosaccharides derived from interferon-alpha2b and interferon-alpha14c produced by sendai-virus-induced human peripheral blood leukocytes. | we have previously isolated and partially characterised the components of a highly purified interferon-alpha (ifn-alpha) preparation produced by sendai-virus-induced human peripheral blood leukocytes. nine ifn-alpha species were identified, and two of these were found to be glycosylated [nyman, t. a., tölö, h., parkkinen, j. & kalkkinen, n. (1998) identification of nine interferon-alpha subtypes produced by sendai-virus-induced human peripheral blood leukocytes, biochem. j. 329, 295-302]. here, ... | 1998 | 9654101 |
| human papillomavirus 16 e6 oncoprotein binds to interferon regulatory factor-3 and inhibits its transcriptional activity. | interferon regulatory factor-3 (irf-3) was found to specifically interact with hpv16 e6 in a yeast two-hybrid screen. irf-3 is activated by the presence of double-stranded rna or by virus infection to form a stable complex with other transcriptional regulators that bind to the regulatory elements of the ifnbeta promoter. we show that irf-3 is a potent transcriptional activator and demonstrate that hpv16 e6 can inhibit its transactivation function. the expression of hpv16 e6 in primary human kera ... | 1998 | 9649509 |
| exogenous and endogenous il-10 regulate ifn-alpha production by peripheral blood mononuclear cells in response to viral stimulation. | il-10 is an important regulator of the production of proinflammatory cytokines. its effect on ifn-alpha production, however, has not been reported. in this study, pbmc from healthy donors were stimulated with virus in the presence of il-10. human il-10 (hil-10) caused reductions in both the frequency of ifn-alpha-producing cells (ipc) and bulk ifn in response to herpes simplex virus type-1 (hsv-1), sendai virus, newcastle disease virus, and vesicular stomatitis virus. the inhibitory effect occur ... | 1998 | 9637497 |
| rapid simultaneous diagnosis of infections with respiratory syncytial viruses a and b, influenza viruses a and b, and human parainfluenza virus types 1, 2, and 3 by multiplex quantitative reverse transcription-polymerase chain reaction-enzyme hybridization assay (hexaplex). | a multiplex quantitative reverse transcription-polymerase chain reaction-enzyme hybridization assay (hexaplex; prodesse, milwaukee) was developed and used to rapidly detect and quantitate rna of respiratory syncytial viruses a and b, influenza viruses a and b, and human parainfluenza virus types 1, 2, and 3 in nasal wash specimens in a single test. primers and probes originated from highly conserved regions of each viral genome. six and a half primer pairs were mixed for the simultaneous detecti ... | 1998 | 9636869 |
| prolonged survival of recipient rats with fas-ligand-transfected liver allografts by using hvj-liposome. | 1998 | 9636379 | |
| the various sendai virus c proteins are not functionally equivalent and exert both positive and negative effects on viral rna accumulation during the course of infection. | recombinant sendai viruses were prepared which cannot express their cprime, c, or cprime plus c proteins due to mutation of their respective start codons ([cprime-minus], [c-minus] and [double mutant], respectively). the [cprime-minus] and [c-minus] stocks were similar to that of wild-type (wt) virus in virus titer and plaque formation, whereas the double-mutant stock had a much-reduced pfu or 50% egg infective dose/particle ratio and produced very small plaques. relative to the wt virus infecti ... | 1998 | 9621061 |
| persistent gene expression in rat liver in vivo by repetitive transfections using hvj-liposome. | most viral vectors are highly immunogenic and are of limited use for somatic gene therapy that requires repetitive administrations. we have developed a highly efficient gene transduction procedure useful for repetitive transfections using liposome containing hemagglutinating virus of japan (hvj-liposome). the escherichia coli beta-galactosidase (beta-gal) gene was embodied in hvj-liposome, and introduced directly into the caudal lobe of rat liver that was transiently isolated from a systemic cir ... | 1998 | 9614569 |
| contamination of mouse-adapted influenza virus with sendai virus. | in our laboratory animal facility, sendai virus (hvj) contamination occurred in a negative flow rack used for experimental infection with 4 strains of mouse-adapted influenza virus (inf.v). anti-hvj antibody (ab) was detected in 35/42 mice in the rack. to specify the strain of inf.v contaminated with hvj, experimental infection was performed by using a, b and d strains of inf.v in each vinyl isolator. anti-hvj ab was detected in all mice infected with a strain at day 28 post-infection. as a resu ... | 1998 | 9606426 |
| detection of genes of rna viruses from freshly biopsied gastric mucosa by reverse transcription polymerase chain reaction. | the reverse transcription polymerase chain reaction (rt-pcr) was performed to detect genes of rna viruses in the freshly biopsied gastric mucosa of seven patients with low gastric acidity. although nucleoprotein genes of sendai virus and hemmaglutinin genes of influenza virus a were not detected, nucleoprotein genes of influenza virus b were detected in samples from three of the seven patients. the first patient had had antrectomy and vagotomy for gastric ulcer, the second patient was receiving ... | 1998 | 9605941 |
| sendai virus c proteins are categorically nonessential gene products but silencing their expression severely impairs viral replication and pathogenesis. | the p/c mrna of sendai virus (sev), a prototypic member of the family paramyxoviridae in the mononegavirales superfamily comprising a large number of nonsegmented negative strand rna viruses, encodes a nested set of accessory proteins, c', c, y1 and y2, referred to collectively as c proteins, initiating, respectively, at acg/81 and augs/114, 183, 201 in the +1 frame relative to the orf of phospho (p) protein, the smaller subunit of rna polymerase. among them, c is the major species expressed in ... | 1998 | 9605405 |
| the roles of individual cysteine residues of sendai virus fusion protein in intracellular transport. | the role of intramolecular disulfide bonds in the fusion (f) protein of sendai virus was studied. the 10 cysteine residues were changed to serine residues using site-directed mutagenesis. none of the cysteine mutant f proteins reacted with a monoclonal antibody specific for the mature conformation of the f protein, but eight of ten mutants reacted with an immature conformation-specific monoclonal antibody. the transport of these mutant proteins to the cell surface was drastically reduced. all of ... | 1998 | 9603994 |
| pulmonary disease models induced by in vivo hemagglutinating virus of japan liposome-mediated endothelin-1 gene transfer. | overproduction and overexpression of endothelin-1 (et-1) have been reported to contribute to the pathophysiology of pulmonary diseases, including pulmonary fibrosis, obliterative bronchiolitis, and primary pulmonary hypertension. to determine whether et-1 contributes to the pathogenesis of pulmonary disease, we locally overexpressed et-1 using an in vivo uv-inactivated hemagglutinating virus of japan (hvj) liposome-mediated gene transfer system. plasmid dna of et-1 (pme18fc preproet-1) and high ... | 1998 | 9595474 |
| influence of respiratory tract viral infection on endothelin-1-induced modulation of cholinergic nerve-mediated contractions in murine airway smooth muscle. | the effects of endothelin-1 (et-1) and sarafotoxin s6c (s6c) on cholinergic contractions elicited by electrical field stimulation (efs) were examined in mouse tracheal preparations from healthy animals and from animals infected with parainfluenza-1 (p-1) virus. s6c (an etb-selective agonist) and et-1 caused marked eta and/or etb receptor-mediated potentiation of efs-induced contraction in tracheal tissue from both groups. despite the fact that such infection is known to markedly alter et recepto ... | 1998 | 9595442 |
| hvj (sendai virus) liposome-mediated gene transfer: current status and future perspectives (review). | haemagglutinating virus of japan (hvj; sendai virus), a member of the mouse paramyxovirus family, has been combined with liposomes to produce a novel gene transfer system, namely hvj liposomes. this vector system is defined as a <hybrid vector>, constructed with inactivated viral particles and non-viral (artificial) multi- or unilamellar liposomes containing gene expression cassettes and has several advantages in comparison with other viral or non-viral systems. many studies have shown that this ... | 1998 | 9592186 |
| probe transfer with and without membrane fusion in a fluorescence fusion assay. | an analysis of the r18 fusion assay was made during the fusion of the sendai virus with erythrocyte ghosts. the increase in r18 fluorescence, reflecting the interaction process, was evaluated in terms of the different processes that in principle may contribute to this increase, that is, monomeric probe transfer, hemifusion, and complete fusion. to this end, the kinetics of the r18-labeled lipid mixing were compared to those obtained with an assay in which the fusion-monitoring probe, eosin-malei ... | 1998 | 9585563 |
| pseudotype formation of moloney murine leukemia virus with sendai virus glycoprotein f. | mixed infection of cells with both moloney murine leukemia virus (momlv) and related or heterologous viruses produces progeny pseudotype virions bearing the momlv genome encapsulated by the envelope of the other virus. in this study, pseudotype formation between momlv and the prototype parainfluenza virus sendai virus (sv) was investigated. we report for the first time that sv infection of momlv producer cells results in the formation of momlv(sv) pseudotypes, which display a largely extended ho ... | 1998 | 9573308 |
| sendai virus-like particles devoid of haemagglutinin-neuraminidase protein infect cells via the human asialoglycoprotein receptor. | virus-like particles with genetically defined envelope proteins were generated from cdna in order to examine the requirement of sendai virus haemagglutinin-neuraminidase (hn) protein for particle formation, and the role of fusion protein (f) in receptor binding and membrane fusion. characterization of particles devoid of hn protein showed that particle formation was unimpaired by the absence of hn protein, indicating that hn protein is dispensable for virus assembly and budding. infection studie ... | 1998 | 9568961 |
| the short sendai virus leader region controls induction of programmed cell death. | the replication of nonsegmented minus-strand rna genomes, like that of sendai paramyxovirus (sev), are controlled by the short leader regions present at each end of the linear genomes and antigenomes; the left and right promoters (pl and pr), respectively. wild-type sev is highly cytopathic in cell culture, because it induces programmed cell death (pcd). we have found that a recombinant sev (rsevgp42), in which the first 42 nt of le+ sequences at pl were replaced with the equivalent sequences of ... | 1998 | 9568033 |
| the hvj liposome method. | recent advancement of gene technology allows us a practical approach to gene therapy. among various in vivo gene transfer techniques available, the hvj liposome method is an efficient procedure which could target the glomerular cells. using this method, hvj-mediated cell fusion activity enables us to introduce genetic materials directly into the cytosol without degradation. in addition, cointroduction of non-histone nuclear protein, high-mobility group (hmg-1), efficiently facilitates migration ... | 1998 | 9567220 |
| virus-dependent phosphorylation of the irf-3 transcription factor regulates nuclear translocation, transactivation potential, and proteasome-mediated degradation. | the interferon regulatory factors (irf) consist of a growing family of related transcription proteins first identified as regulators of the alpha beta interferon (ifn-alpha/beta) gene promoters, as well as the interferon-stimulated response element (isre) of some ifn-stimulated genes. irf-3 was originally identified as a member of the irf family based on homology with other irf family members and on binding to the isre of the isg15 promoter. irf-3 is expressed constitutively in a variety of tiss ... | 1998 | 9566918 |
| gene transfer and kidney disease. | there is little doubt that molecular biological intervention therapy has come of age and its potential is arousing tremendous excitement. a gene transfer technique, the hvj-liposome method, is now applicable as a tool for the dissection of molecular aspects in the pathophysiology of renal diseases, and for gene therapy in experimental glomerulonephritis. overexpressed transforming growth factor (tgf)-beta in the normal rat glomeruli, by gene transfer of tgf-beta cdna, leads to glomerulosclerosis ... | 1998 | 9561480 |
| a role for the sendai virus p protein trimer in rna synthesis. | the sev p protein is found as a homotrimer (p3) when it is expressed in mammalian cells, and trimerization is mediated by a predicted coiled-coil motif which maps within amino acids (aa) 344 to 411 (the boxa region). the bacterially expressed protein also appears to be trimeric, apparently precluding a role for phosphorylation in the association of the p monomers. i have examined the role of p trimerization both in the protein's interaction with the nucleocapsid (n:rna) template and in the prote ... | 1998 | 9557717 |
| [in vivo gene transfection with heat shock protein 70 enhances myocardial tolerance to ischemia-reperfusion injury in rat]. | 1998 | 9557285 | |
| inhibitory effect of interleukin-10 on human leukocyte interferon-alpha production by sendai virus. | treatment of human peripheral blood leukocytes (hpbl) with sendai virus induces significant production of human interferon-alpha (ifn-alpha). addition of human recombinant interleukin-10 (il-10) to hpbl in vitro prior to treatment with sendai virus resulted in considerable inhibition of ifn-alpha production. downregulation of ifn-alpha production was il-10 concentration-dependent and observed at il-10 concentrations of as low as 0.05 ng/ml, with a median effective dose (ed50) of about 5 ng/ml. i ... | 1998 | 9557212 |
| attenuation of virus-induced airway dysfunction in rats treated with imiquimod. | viral respiratory infections cause acute airway abnormalities consisting of inflammation and physiological dysfunction in both animals and humans. it is likely that inflammatory cell products, such as cytokines, contribute substantially to viral-induced airway dysfunction. we hypothesized that imiquimod, an immune response enhancing agent that induces interferon-alpha, would attenuate the development of airway dysfunction during acute viral illness in rats. adult brown norway rats were inoculate ... | 1998 | 9551732 |
| tap peptide transporter-independent presentation of heat-killed sendai virus antigen on mhc class i molecules by splenic antigen-presenting cells. | immunization of c57bl/6 (b6) mice with heat-killed sendai virus generates a sendai virus-specific cd8+ t cell response. this suggests that apc have the capacity to take up and present exogenous (nonreplicative) sendai virus ag on mhc class i molecules. little is known about the intracellular requirements for processing of this form of ag and its presentation on mhc class i. therefore, we have studied the processing and presentation of heat-killed sendai virus ag on mhc class i molecules in splen ... | 1997 | 9548476 |
| [hemagglutinating virus of japan]. | 1997 | 9545873 | |
| [establishment of sendai virus gene manipulation and its applications]. | 1997 | 9545859 | |
| large quantity production with extreme convenience of human sdf-1alpha and sdf-1beta by a sendai virus vector. | we describe a robust expression of human stromal cell-derived factor-1alpha (sdf-1alpha) and sdf-1beta, the members of cxc-chemokine family, with a novel vector system based upon sendai virus, a non-segmented negative strand rna virus. recombinant sdf-1alpha and sdf-1beta were detected as a major protein species in culture supernatants, reached as high as 10 microg/ ml. this remarkable enrichment of the products allowed us to use even the crude supernatants as the source for biological and antiv ... | 1998 | 9541016 |
| liposome-mediated gene transfer. | 1995 | 9532561 | |
| cluster headache associated with parainfluenza virus, preceded and succeeded by migraine. | a serologically proven case of parainfluenza viral infection was associated with the onset and disappearance of cluster headache. the patient had long-standing migraine that ceased during the cluster headache period and recurred when the latter stopped. possibly, the virus was neurally transmitted to the trigeminal-autonomic system, creating an inflammatory response that transiently precipitated cluster headache and obliterated migraine. | 1998 | 9529770 |
| evaluation of a single dilution elisa system for detection of seroconversion to bovine viral diarrhea virus, bovine respiratory syncytial virus, parainfluenza-3 virus, and infectious bovine rhinotracheitis virus: comparison with testing by virus neutralization and hemagglutination inhibition. | a single-dilution quantitative enzyme-linked immunosorbent assay (elisa) system, based on commercial elisa kits, for the simultaneous detection of seroconversion to bovine viral diarrhea virus (bvdv), bovine respiratory syncytial virus (brsv), parainfluenza-3 virus (pi3v), and infectious bovine rhinotracheitis virus (ibrv) was evaluated by testing acute and convalescent serum pairs from 564 cattle in 145 outbreaks of respiratory disease. seroconversion to bvdv, brsv, pi3v and ibrv was detected i ... | 1998 | 9526859 |
| the activity of sendai virus genomic and antigenomic promoters requires a second element past the leader template regions: a motif (gnnnnn)3 is essential for replication. | the paramyxovirus genome, a nonsegmented, negative-polarity, single-stranded rna of approximately 15 kb, contains six transcription units flanked at the 3' and 5' ends by a short (approximately 50- to 60-nucleotide) extracistronic sequence, dubbed the positive and negative leader regions. these leader template regions, present at the 3' end of the genome and the antigenome, have been shown to contain essential signals governing rna replication activity. whether they are sufficient to promote rep ... | 1998 | 9525637 |
| increased induction of apoptosis by a sendai virus mutant is associated with attenuation of mouse pathogenicity. | an avirulent mutant of sendai virus, ohita-mvc11 (mvc11), was generated from a highly virulent field strain, ohita-m1 (m1), through successive passages in llc-mk2 cell cultures (m. itoh, y. isegawa, h. hotta, and m. homma, j. gen. virol. 78:3207-3215, 1997). in llc-mk2 cells, mvc11 induced a high degree of apoptotic cell death that was demonstrated by chromatin condensation of the nucleus and dna fragmentation, and production of mvc11 declined markedly after prolonged culture. on the other hand, ... | 1998 | 9525613 |
| gene therapy in cardiovascular surgery. | 1998 | 9524998 | |
| the replicative complex of paramyxoviruses: structure and function. | 1998 | 9520998 |