Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| rhizopus oryzae fungus cells producing l(+)-lactic acid: kinetic and metabolic parameters of free and pva-cryogel-entrapped mycelium. | spores of the filamentous fungus rhizopus oryzae were entrapped in macroporous poly(vinyl alcohol) cryogel (pva-cryogel). to prepare immobilised biocatalyst capable of producing l(+)-lactic acid (la), the fungus cells were cultivated inside the carrier beads. the growth parameters and metabolic activity of the suspended (free) and immobilised cells producing la in a batch process were comparatively investigated. the immobilised cells possessed increased resistance to high concentrations of accum ... | 2006 | 16523285 |
| the family 21 carbohydrate-binding module of glucoamylase from rhizopus oryzae consists of two sites playing distinct roles in ligand binding. | the starch-hydrolysing enzyme ga (glucoamylase) from rhizopus oryzae is a commonly used glycoside hydrolase in industry. it consists of a c-terminal catalytic domain and an n-terminal starch-binding domain, which belong to the cbm21 (carbohydrate-binding module, family 21). in the present study, a molecular model of cbm21 from r. oryzae ga (rogacbm21) was constructed according to pssc (progressive secondary structure correlation), modified structure-based sequence alignment, and site-directed mu ... | 2006 | 16509822 |
| state variables monitoring by in situ multi-wavelength fluorescence spectroscopy in heterologous protein production by pichia pastoris. | state variables throughout non-induced and induced cultivations of pichia pastoris for the heterologous rhizopus oryzae lipase (rol) production were monitored with a multi-wavelength on-line fluorescence sensor. based on this work, the use of in situ multi-wavelength fluorometry combined with chemometrics models (pls-1 models) provided a quantitative prediction of biomass and substrates (glycerol and methanol) during non-induced and induced rol production. the mean prediction errors for both var ... | 2006 | 16488501 |
| [heteroexpression of rhizopus arrhizus delta6-fatty acid desaturase gene in pichia pastoris]. | delta6-fatty acid desaturase is a membrane-bound enzyme, which is rate-limiting for the biosynthesis of polyunsaturated fatty acids. a cdna sequence putatively encoding a delta6-fatty acid desaturase was isolated from rhizopus arrhizus nk300037 using rt-pcr and race methods in our previous work. sequence and function analysis indicated that this sequence was a novel delta6-fatty acid desaturase gene which had an open reading frame of 1377bp coding 458 amino acids of 52kd. the methylotrophic yeas ... | 2005 | 16468339 |
| association constants of pb2+ with binding sites of fungal biomass using metal-based titrations. | biosorption is perceived as an alternative method for toxic heavy metal removal/recovery from aqueous effluents. this work focused on derivation of some key quantitative physico-chemical characteristics of a representative biosorbent material required for its further effective exploitation. the newly developed acid-base and metal-based titrator allowed the characterization of the chemisorption active sites of rhizopus arrhizus biomass and the study of their metal affinity. this experimental appr ... | 2006 | 16457181 |
| rapid susceptibility testing of medically important zygomycetes by xtt assay. | the xtt colorimetric assay quantifies fungal growth by measuring fungal metabolism and has been used successfully for susceptibility testing of aspergillus species after 24 and 48 h of incubation. in the present study using 14 clinical isolates of zygomycetes (rhizopus oryzae [5 isolates], cunninghamella spp. [3 isolates], mucor spp. [3 isolates], and absidia corymbifera [3 isolates]), significant metabolic activity was demonstrated before visual or spectrophotometric detection of fungal growth ... | 2006 | 16455912 |
| molecular identification of zygomycetes from culture and experimentally infected tissues. | mucormycosis is an emerging infection associated with a high mortality rate. identification of the causative agents remains difficult and time-consuming by standard mycological procedures. in this study, internal transcribed spacer (its) sequencing was validated as a reliable technique for identification of zygomycetes to the species level. furthermore, species identification directly from infected tissues was evaluated in experimentally infected mice. fifty-four zygomycetes strains belonging to ... | 2006 | 16455881 |
| [mycoses of the head and neck]. | the ever growing number of immunocompromized patients and increasing travel to areas where fungal diseases are endemic explain why in recent years mycoses have emerged as important infections in clinical practice. it is essential that pathologists and clinicians be knowledgeable about them. | 2005 | 16439931 |
| [cutaneous mucormycosis and diabetes: about one observation]. | mucormycosis is a rare opportunistic infection but a fulminant disease. varying clinical forms have been described, including cutaneous localisations which are mainly observed in diabetic and burned patients. the cutaneous lesions induced by the mucormycosis affection are often atypical and gangrenous. we report a case of cutaneous mucormycosis in a tunisian patient with diabetic ketoacidosis. he developed cutaneous necrotic lesions at the low limb. the diagnosis of mucormycosis was not initiall ... | 2005 | 16425710 |
| functional characterization of front-end desaturases from trypanosomatids depicts the first polyunsaturated fatty acid biosynthetic pathway from a parasitic protozoan. | a survey of the three kinetoplastid genome projects revealed the presence of three putative front-end desaturase genes in leishmania major, one in trypanosoma brucei and two highly identical ones (98%) in t. cruzi. the encoded gene products were tentatively annotated as delta8, delta5 and delta6 desaturases for l. major, and delta6 desaturase for both trypanosomes. after phylogenetic and structural analysis of the deduced proteins, we predicted that the putative delta6 desaturases could have del ... | 2006 | 16403015 |
| cytokine responses and regulation of interferon-gamma release by human mononuclear cells to aspergillus fumigatus and other filamentous fungi. | there is substantial evidence that the production of proinflammatory cytokines is important in host resistance to invasive aspergillosis. knowledge of the host response towards other filamentous fungi is scarce, as most studies have focused on aspergillus fumigatus. in addition, interferon-gamma (ifngamma) plays a crucial role in the control of invasive aspergillosis, but little is known about the regulation of ifngamma after stimulation of mononuclear cells by a. fumigatus. cytokine responses t ... | 2005 | 16396246 |
| secretion of pro- and mature rhizopus arrhizus lipases by pichia pastoris and properties of the proteins. | the lipases of rhizopus spp. share a high 1,3-regiospecificity toward triacylglycerols, which makes them important enzymes in lipid modification. in the present study, the extracellularly active production of recombinant rhizopus arrhizuslipase was carried out with genes encoding the mature region (mral) and the mral having the prosequence (proral) in pichia pastoris. two transformed p. pastoris clones containing the multicopy of mral and proral genes were separately selected for the production ... | 2006 | 16382184 |
| post harvest spoilage of sweetpotato in tropics and control measures. | sweetpotato storage roots are subjected to several forms of post harvest spoilage in the tropical climate during transportation from farmers' field to market and in storage. these are due to mechanical injury, weight loss, sprouting, and pests and diseases. sweetpotato weevil is the single most important storage pest in tropical regions for which no control measures or resistant variety are yet available. several microorganisms (mostly fungi) have been found to induce spoilage in stored sweetpot ... | 2005 | 16371331 |
| an improved method for sn-2 position analysis of triacylglycerols in edible oils and fats based on immobilised lipase d (rhizopus delemar). | the use of lipase d (rhizopus delemar) immobilised on microporous polypropylene as a replacement for the standard pancreatic lipases used in the stereospecific sn-2 position analysis of triacylglycerols from edible oils and fats is studied. excellent hydrolysis characteristics are obtained in hexane/methanol solvents at reaction temperatures up to 60 degrees c with hydrolysis times of only 10-20 min. the favourable conditions for the hydrolysis reaction allow fats with higher melting points to b ... | 2006 | 16368101 |
| production of fumaric acid in 20-liter fermentors. | the conditions necessary for the production of fumaric acid in 20-liter fermentors by fermentation of glucose with rhizopus arrhizus strain nrrl 2582 were determined. continuous neutralization of fumaric acid was necessary for optimal yields. yields of the calcium salt were in excess of 65 g of fumaric acid from 100 g of sugar consumed during fermentation of sugar concentrations of 10 to 16%. conditions established for calcium fumarate production include a simple mineral salts medium, 0.5 v:v:mi ... | 1962 | 16349614 |
| biochemical aspects of fumaric acid accumulation by rhizopus arrhizus. | the accumulation and excretion of fumaric acid, and to a lesser extent malic and succinic acids, by rhizopus arrhizus occurs under aerobic conditions in a high-glucose medium containing a limiting amount of nitrogen and a neutralizing agent (caco(3)). an overall four-carbon dicarboxylic acid molar yield of up to 145% (moles of acid produced per mole of glucose utilized) is obtained after incubation for 4 to 5 days. evidence is presented that fumarate is synthesized from pyruvate via a carboxylat ... | 1986 | 16347099 |
| pressate from peat dewatering as a substrate for bacterial growth. | this study considered the possibility of using water expressed during the drying of fuel-grade peat as a substrate for microbial growth. highly humified peat pressed for 2.5 min at 1.96 mpa produced water with a chemical oxygen demand of 690 mg/liter. several biological compounds could be produced by using the organic matter in expressed peat water as a substrate. these included polymers such as chitosan, contained in the cell wall of rhizopus arrhizus, and two extracellular polysaccharides, xan ... | 1985 | 16346834 |
| uptake of metal ions by rhizopus arrhizus biomass. | rhizopus arrhizus biomass was found to absorb a variety of different metal cations and anions but did not absorb alkali metal ions. the amount of uptake of the cations was directly related to ionic radii of la, mn, cu, zn, cd, ba, hg, pb, uo(2), and ag. the uptake of all the cations is consistent with absorption of the metals by sites in the biomass containing phosphate, carboxylate, and other functional groups. the uptake of the molybdate and vanadate anions was strongly ph dependent, and it is ... | 1984 | 16346521 |
| microbial transformation of macrocyclic trichothecenes. | a resting culture of rhizopus arrhizus (atcc 11145) transformed verrucarin a into 16-hydroxyverrucarin a, whereas r. arrhizus transformed verrucarin b into a mixture of 16-hydroxyverrucarin b and 3'-hydroxyverrucarin a. relative to verrucarins a and b, the 16-hydroxy derivatives showed marked increases in activity, as tested in vivo against p388 mouse leukemia. | 1983 | 16346370 |
| heterologous production of functional forms of rhizopus oryzae lipase in escherichia coli. | to date, expression of the lipase from rhizopus oryzae (rol) in escherichia coli always led to the formation of inclusion bodies and inactive protein. however, the production of active rol and its precursor prorol in soluble form was achieved when e. coli origami(de3) and pet-11d were used as expression systems. | 2005 | 16332903 |
| stabilization of the initial electrochemical potential for a metal-based potentiometric titration study of a biosorption process. | an interactive metal-based potentiometric titration method has been developed using an ion selective electrode for studying the sorption of metal cations. the accuracy of this technique was verified by analyzing the metal sorption mechanism for the biomass of rhizopus arrhizus fungus and diatomite, two dissimilar materials (organic and mineral, strong sorbent and weak sorbent) of a different order of cation exchange capacity. the problem of the initial electrochemical potential was addressed ide ... | 2006 | 16325652 |
| effect of the dates of extraction on the quality of potato pulp. | the objectives of this study were to evaluate the fundamental properties of potato pulp and to explore its potential uses. lactic acid bacteria were the dominant microbes in potato pulp over the season (maximum being 10(8)/g). the water content in potato pulp was approximately 80% in all season. starch and fiber were the main components of potato pulp, amounting to 80% of the dried matter regardless of the season. the fermentation of potato pulp by rhizopus oryzae progressed only in the potato p ... | 2006 | 16324844 |
| [mucormycosis caused by rhizopus oryzae in a patient with atypical diabetes]. | 2005 | 16324575 | |
| cerebral zygomycosis. | fifty-six patients with cerebral zygomycosis (mucormycosis) were seen during the period 1971-2001 in two tertiary care hospitals located in south india with tropical climate and catering to neurological diseases. forty-four patients had rhinocerebral and twelve patients had isolated central nervous system (cns) zygomycosis. of these, ten were culture proven (rhizopus oryzae in eight and mucor in two); 30 were diagnosed as probable and 16 were diagnosed possible; mixed infections were seen in thr ... | 2005 | 16262876 |
| pcr based identification and discrimination of agents of mucormycosis and aspergillosis in paraffin wax embedded tissue. | invasive fungal infections are often diagnosed by histopathology without identification of the causative fungi, which show significantly different antifungal susceptibilities. | 2005 | 16254108 |
| olive-mill wastewaters: a promising substrate for microbial lipase production. | the present study investigated the valorization of olive-mill wastewater (omw) by its use as a possible growth medium for the microbial production of extra-cellular lipase. to this end, strains of geotrichum candidum (nrrl y-552 and y-553), rhizopus arrhizus (nrrl 2286 and isrim 383), rhizopus oryzae (nrrl 6431), aspergillus oryzae (nrrl 1988 and 495), aspergillus niger (nrrl 334), candida cylindracea (nrrl y-17506) and penicillium citrinum (nrrl 1841 and 3754, isrim 118) were screened. all stra ... | 2006 | 16236495 |
| chitosan productivity enhancement in rhizopus oryzae ypf-61a by d-psicose. | the effect of the rare sugar d-psicose on chitosan production by rhizopus oryzae was studied. the fungus was not able to utilize d-psicose as a sole source of carbon, either for germination of the spores or for growth of the vegetative cells. in a medium containing a low amount of d-glucose, however, d-psicose supplementation between 5 and 12 g/l caused enhancement of the productivity of chitinous substances, especially chitosan, in the cell walls. substantial changes in the chitosan molecule we ... | 2003 | 16233408 |
| cell surface engineering of yeast: construction of arming yeast with biocatalyst. | a cell surface engineering system of yeast saccharomyces cerevisiae has been established and novel yeasts armed by biocatalysts (enzymes-glucoamylase, alpha-amylase, cm-cellulase, beta-glucosidase, and lipase), termed "arming yeasts", were constructed. the gene encoding rhizopus oryzae glucoamylase with its secretion signal peptide was fused with the gene encoding the c-terminal half of yeast alpha-agglutinin and expressed in s. cerevisiae. glucoamylase was shown to be displayed on the cell surf ... | 2000 | 16232831 |
| biodiesel fuel production from plant oil catalyzed by rhizopus oryzae lipase in a water-containing system without an organic solvent. | a new enzymatic method of synthesizing methyl esters from plant oil and methanol in a solvent-free reaction system was developed. it is anticipated that such plant oil methyl esters can be used as a biodiesel fuel in the future. lipase from rhizopus oryzae efficiently catalyzed the methanolysis of soybean oil in the presence of 4-30 wt% water in the starting materials; however the lipase was nearly inactive in the absence of water. the methyl ester (me) content in the reaction mixture reached 80 ... | 1999 | 16232675 |
| optimization of n variable biological experiments by evolutionary operation-factorial design technique. | since the traditional approach to optimization of biological systems based on the single variable search technique is incapable of detecting the true optimum when a number of chemicals are used together as inducers, the evolutionary operation (evop)-factorial design technique was applied successfully to optimize the concentrations of vitamin (biotin), metal ion (cacl(2)) and plant hormone (naa) for maximizing enzyme (protease) production by rhizopus oryzae (ro iit rb-13, nrrl-21498) in solid sta ... | 1999 | 16232455 |
| influence of autoclaved fungal materials on spearmint (mentha spicata l.) growth, morphogenesis, and secondary metabolism. | the influence of autoclaved fungal materials such as culture filtrate, freeze-dried mycelium (fdm), mycelium suspension, and spore suspension (ss) on the growth, morphogenesis, and carvone production of spearmint (mentha spicata l.) plants was studied. fungal materials were either applied as a drench or spray on the plants. spearmint plants (cv. "294099") drenched with ss (1 x 10(8) spores/ml) of trichoderma reesei showed no significant differences in leaf numbers, root numbers, or shoot numbers ... | 2005 | 16222794 |
| production of lactic acid and fungal biomass by rhizopus fungi from food processing waste streams. | this study proposed a novel waste utilization bioprocess for production of lactic acid and fungal biomass from waste streams by fungal species of rhizopus arrhizus 36017 and r. oryzae 2062. the lactic acid and fungal biomass were produced in a single-stage simultaneous saccharification and fermentation process using potato, corn, wheat and pineapple waste streams as production media. r. arrhizus 36017 gave a high lactic acid yield up to 0.94-0.97 g/g of starch or sugars associated with 4-5 g/l o ... | 2005 | 16208461 |
| molecular analysis of an nad-dependent alcohol dehydrogenase from the zygomycete mucor circinelloides. | nad-dependent alcohol dehydrogenase (adh) activity was detected mainly in the cytosol of aerobically cultured mycelium and in anaerobically grown yeast cells of mucor circinelloides. adh levels were about 2.5-fold higher in yeast cells than in mycelium; zymogram analysis suggested that the same adh enzyme is produced in both developmental stages. the enzyme, named adh1, was purified to homogeneity from yeast cells, using ion-exchange and affinity chromatography. the active adh1 appears to be a h ... | 2005 | 16179992 |
| circadian rhythms in neurospora crassa: clock gene homologues in fungi. | computer-based analysis of a total of 17 filamentous fungal and yeasts genomes has shown: (1) homologues of frq, wc-1, wc-2, and vvd, key gene components of the neurospora crassa clock, are present in magnaporthe grisea, gibberella zeae, and podospora anserina, suggesting an frq-based oscillator in these organisms; (2) some fungal species that are more distantly related to neurospora, such as rhizopus oryzae do not appear to have frq homologues; (3) many fungal species that do not appear to cont ... | 2005 | 16154782 |
| [mycoses of the head and neck]. | in recent years, mycoses have emerged as important infections in clinical practice. this phenomenon is explained by the ever growing number of immunocompromised patients and the increasing number of people travelling in areas where fungal diseases are endemic. head and neck infections are common in disseminated mycoses and may simulate carcinoma or cause upper airway obstruction. the most frequent causative yeasts or yeast-like organisms include candida albicans, cryptococcus neoformans, histopl ... | 2005 | 16142162 |
| inhibition of non-homologous end joining and integration of dna upon transformation of rhizopus oryzae. | site-directed integration of dna in the fungus rhizopus has long been problematic because linearized plasmids used for transformation tend to replicate in high-molecular-weight concatenated structures, and rarely integrate into the chromosome. this work examines the methods that might interfere with the multimerization process, select against plasmids that had recircularized, and encourage strand invasion, hopefully leading to plasmid integration. in vitro methods were used to determine if the s ... | 2005 | 16133163 |
| the active component in the flax-retting system of the zygomycete rhizopus oryzae sb is a family 28 polygalacturonase. | the zygomycete rhizopus oryzae sb is a very efficient organism for retting of flax, the initial microbiological step in the process of making linen. an extracellular polygalacturonase, when isolated could perform retting, and therefore probably is the key component in the retting system of r. oryzae. this was purified and characterized. the purified enzyme has a molecular mass of 37,436 da from mass spectrometric determination, an isoelectric point of 8.4, and has non-methylated polygalacturonic ... | 2005 | 16133102 |
| zygomycosis. | zygomycosis is an invasive mycotic disease caused by fungi in the class zygomycetes. within this class, the most common species associated with infection include absidia corymbifera, apophysomyces elegans, cunninghamella bertholletiae, rhizomucor pusillus, rhizopus microsporus var. rhizopodiformis, and rhizopus arrhizus. these fungi all produce characteristic hyphal forms in tissue that are variable in width, produce haphazard branching, and lack septation. although these fungi are ubiquitous in ... | 2004 | 16088464 |
| enhanced reactivity of rhizopus oryzae lipase displayed on yeast cell surfaces in organic solvents: potential as a whole-cell biocatalyst in organic solvents. | immobilization of enzymes on some solid supports has been used to stabilize enzymes in organic solvents. in this study, we evaluated applications of genetically immobilized rhizopus oryzae lipase displayed on the cell surface of saccharomyces cerevisiae in organic solvents and measured the catalytic activity of the displayed enzyme as a fusion protein with alpha-agglutinin. compared to the activity of a commercial preparation of this lipase, the activity of the new preparation was 4.4 x 10(4)-fo ... | 2005 | 16085821 |
| in vitro susceptibility of 15 strains of zygomycetes to nine antifungal agents as determined by the nccls m38-a microdilution method. | the in vitro susceptibility of 15 strains of six genera of zygomycetes including rhizopus oryzae (rhizopus arrhizus), rhizopus stolonifer, mucor circinelloides (three), absidia corymbifera (three), rhizomucor pusillus (three), cunninghamella bertholletiae (two), and syncephalastrum racemosum (two) to nine antifungal agents were determined by the nccls m38-a broth microdilution method. geometric means of the minimal inhibitory concentrations (mic) were: amphotericin b 0.07 mg l(-1) (range 0.03-0. ... | 2005 | 15982206 |
| lead biosorption study with rhizopus arrhizus using a metal-based titration technique. | acid-base and metal-based potentiometric titration methods were used to analyze sorption mechanisms of lead by rhizopus arrhizus fungal biomass. biosorption was not considered globally but as the result of successive sorption reactions on various binding sites with different selectivities. precipitation occurred rapidly when lead concentration increased. lead was sorbed essentially by carboxylic groups and by phosphates and sulfonates (less abundant) of the organic matter. the lead affinity to c ... | 2005 | 15979634 |
| genetic identification and detection of human pathogenic rhizopus species, a major mucormycosis agent, by multiplex pcr based on internal transcribed spacer region of rrna gene. | mucormycosis is an invasive opportunistic infection caused by fungi belonging to the order mucorales. due to the lack of laboratory tests, the diagnosis of mucormycosis is notoriously difficult. added with its rapid progression as well as the debilitated state of the patients who contract the disease, mortality is extremely high. | 2005 | 15978416 |
| purification and characterization of a new endo-1,4-beta-d-glucanase from beltraniella portoricensis. | a new endoglucanase, designated bce1, produced by beltraniella portoricensis, was purified from the culture supernatant. the n-terminal amino acid sequence suggests that bce1 belongs to family 45 glycoside hydrolase (family 45 endoglucanase). the molecular mass of bce1 was found to be 40 kda by sodium dodecylsulfate polyacrylamide gel electrophoresis (sds-page). the optimum ph for the carboxymethyl cellulase (cmcase) activity of bce1 was 4.5, and the optimum temperature was 55 degrees c. among f ... | 2005 | 15973054 |
| [study on the repeated batch fermentation of lipase by immobilized rhizopus]. | repeated batch fermentation with rhizopus arrhizus immobilized by polyurethane was optimized in details including the time to replace, the volume of the replaced medium and the optimal composition of the medium to replace. immobilized cells showed stability for repeated use. immobilized cells could be used for 9 batches, lasting 140h in flask and 6 batches in 5l fermenter when the substitute culture medium consisted of (%): soybean flour 3.0, earthnut oil 0.5 and the liquid was replaced by 40% . ... | 2004 | 15969123 |
| strain improvement for tannase production from co-culture of aspergillus foetidus and rhizopus oryzae. | spores from the co-culture of aspergillus foetidus and rhizopus oryzae were subjected to uv, heat and ntg (3-nitro,5-methylguanidine) mutagenesis. a few colonies were screened from the selected media for tannase study. amongst all, the best mutant isolated from the heat treatment (60 degrees c for 60 min) was scpr 337. the maximum yield of gallic acid and tannase in case of mutant strain was 95.2% and 53.6 u/ml with an incubation period of 30 h as compared to wild strain where the incubation per ... | 2006 | 15963714 |
| direct fermentation of potato starch wastewater to lactic acid by rhizopus oryzae and rhizopus arrhizus. | the biochemical kinetic of direct fermentation for lactic acid production by fungal species of rhizopus arrhizus 3,6017 and rhizopus oryzae 2,062 was studied with respect to growth ph, temperature and substrate. the direct fermentation was characterized by starch hydrolysis, accumulation of reducing sugar, and production of lactic acid and fungal biomass. starch hydrolysis, reducing sugar accumulation, biomass formation and lactic acid production were affected with the variations in ph, temperat ... | 2005 | 15947951 |
| peripheral enzymatic deacetylation of chitin and reprecipitated chitin particles. | the enzymatic deacetylation of various chitin preparations was investigated using the fungal chitin deacetylase (cda) isolated from rhizopus oryzae growth medium. specific extracellular enzyme activity after solid state fermentation was 10 times higher than that after submerged fermentation. natural crystalline chitin is a very poor substrate for the enzyme, but showed a five-time better deacetylation after dissolution and reprecipitation. chitin particles, enzymatically deacetylated for only 1% ... | 2006 | 15919204 |
| developing high cell density fed-batch cultivation strategies for heterologous protein production in pichia pastoris using the nitrogen source-regulated fld1 promoter. | a pichia pastoris strain expressing a rhizopus oryzae lipase gene under the transcriptional control of the promoter from the p. pastoris formaldehyde dehydrogenase 1 gene (pfld) was utilized to study the feasibility of this expression system for recombinant protein production using methanol-free fed-batch high cell density cultivations. we have developed a simple and reliable fed-batch strategy using the pfld system based on the use of methylamine and sorbitol as nitrogen and carbon sources, res ... | 2005 | 15918169 |
| extracellular amylase(s) production by fungi botryodiplodia theobromae and rhizopus oryzae grown on cassava starch residue. | the fungi botryodiplodia theobromae and rhizopus oryzae produce extracellular amylase when grown on a liquid medium containing 2% (wn) soluble starch or cassava starch residue(csr) (as starch equivalent), a waste generated after extraction of starch from cassava, as the sole carbon source. using csr as the sole carbon source, the highest amylase activity of 3.25 and 3.8 units (mg, glucose released x ml(-1) x h(-1)) were obtained in shake flask cultures during the late stationary phase of growth ... | 2004 | 15907080 |
| cutaneous mucormycosis in a patient with acute lymphocytic leukemia. | we describe a patient with invasive necrotizing cutaneous mucormycosis caused by rhizopus oryzae. the patient, who had been suffering from acute lymphocytic leukemia (all) for eight months, had erythema and necrosis surrounded by swelling on the dorsum of his left hand. debridement was performed, and microscopic examination of the obtained specimens revealed mucormycosis. because amphotericin b was ineffective, amputation at the left shoulder joint was performed. bone marrow transplantation (bmt ... | 2005 | 15863849 |
| stemodane skeletal rearrangement: chemistry and microbial transformation. | solvolytic rearrangement of the c/d ring system of the tetracyclic diterpenoid stemodinone (2) afforded the compounds 15(13-->12)abeo-13beta-hydroxystemaran-2-one (5) and 15(8-->9)abeo-8beta(h)-12beta-hydroxystachan-2-one (10). terpene 5 possesses a novel diterpene skeleton. oxidation of these compounds yielded their respective diketones. bioconversion of 5 by rhizopus oryzae yielded 15(13-->12)abeo-7beta,13beta-dihydroxystemaran-2-one (18) while microbial transformation of 10 provided 15(8-->9) ... | 2005 | 15845408 |
| alternative splicing produces two endoglucanases with one or two carbohydrate-binding modules in mucor circinelloides. | we previously cloned three endoglucanase genes, rce1, rce2, and rce3, that were isolated from rhizopus oryzae as the first cellulase genes from a member of the subdivision zygomycota. in this study, two cdnas homologous to the rce1 gene, designated the mce1 and mce2 cdnas, were cloned from mucor circinelloides, a member of the subdivision zygomycota. the mce1 cdna encoded an endoglucanase (family 45 glycoside hydrolase) having one carbohydrate-binding module (cbm), designated mce1, and the mce2 ... | 2005 | 15838031 |
| cloning and characterization of a gene rpg1 encoding polygalacturonase of rhizopus oryzae. | the polygalacturonase (pg)-encoding gene (rpg1) of rhizopus oryzae, the causal pathogen of rhizopus rot of mulberry, was cloned and sequenced. pgs were partially purified from incubation mixture of 2% pectin medium and their n-terminal amino acid sequences were determined by a gas-phase protein sequencer. rt-pcr was performed using degenerate primers designed from the amino acid sequences, which resulted in part of a pg-encoding gene being obtained. by 3'-race and tail-pcr analyses, the entire r ... | 2004 | 15757176 |
| combined effect of the methanol utilization (mut) phenotype and gene dosage on recombinant protein production in pichia pastoris fed-batch cultures. | an important number of heterologous proteins have been produced in the methylotrophic yeast pichia pastoris using the alcohol oxidase promoter. two factors that drastically influence protein production and cultivation process development in this system are gene dosage and methanol assimilation capacity of the host strain (mut phenotype). using a battery of four strains which secrete a rhizopus oryzae lipase (rol), the combined effects of gene dosage and mut phenotype on recombinant protein produ ... | 2005 | 15748759 |
| interferon- gamma and granulocyte-macrophage colony-stimulating factor augment the activity of polymorphonuclear leukocytes against medically important zygomycetes. | zygomycetes cause serious invasive infections, predominantly in immunocompromised and diabetic patients with poor prognoses and limited therapeutic options. we compared the antifungal function of human polymorphonuclear leukocytes (pmnls) against hyphae of rhizopus oryzae and r. microsporus, the most frequently isolated zygomycetes, with that against the less frequently isolated absidia corymbifera. we then evaluated the effects of interferon (ifn)- gamma and granulocyte-macrophage colony-stimul ... | 2005 | 15747255 |
| creation of rhizopus oryzae lipase having a unique oxyanion hole by combinatorial mutagenesis in the lid domain. | combinatorial libraries of the lid domain of rhizopus oryzae lipase (rol; phe88xaa, ala91xaa, ile92xaa) were displayed on the yeast cell surface using yeast cell-surface engineering. among the 40,000 transformants in which rol mutants were displayed on the yeast cell surface, ten clones showed clear halos on soybean oil-containing plates. among these, some clones exhibited high activities toward fatty acid esters of fluorescein and contained non-polar amino acid residues in the mutated positions ... | 2005 | 15729555 |
| n-terminal peptide of rhizopus oryzae lipase is important for its catalytic properties. | in a culture medium, the rhizopus oryzae strain produces only one form of lipase, rol32. when the concentrated culture medium was stored at 0 degrees c during several months or kept at 6 degrees c during a few days, we noticed the appearance of a second shorter form of rol32 lacking its n-terminal 28 amino acid (rol29). rol29 was purified to homogeneity and its 21 n-terminal amino acid residues were found to be identical to the 29-49 sequence of rol32. the cleavage of the n-terminal peptide redu ... | 2005 | 15710378 |
| a high-resolution titrator: a new approach to studying binding sites of microbial biosorbents. | the high-resolution potentiometric titration was used as a physico-chemical method to study the acid properties of selected biosorbent materials in order to quantify the functional acidic groups for sorption and to determine their affinities by considering their partial or total ionization equilibrium reactions. the gran's method and the henderson-hasselbach's equation were employed in establishing the partition of the total acidity as associated with strong, weak and very weak acidic chemical a ... | 2004 | 15707630 |
| modelling the effect of ethanol on growth rate of food spoilage moulds. | the effect of ethanol (e) on the radial growth rate (mu) of food spoilage moulds (aspergillus candidus, aspergillus flavus, aspergillus niger, cladosporium cladosporioides, eurotium herbariorum, mucor circinelloides, mucor racemosus, paecilomyces variotii, penicillium chrysogenum, penicillium digitatum, rhizopus oryzae and trichoderma harzianum) was assessed in potato dextrose agar (pda) medium at a(w) 0.99, 25 degrees c. in order to model this effect, the monod type equation described previousl ... | 2005 | 15698687 |
| effect of urea on the enzymatic activity of a lipase entrapped in aot-heptane-water reverse micellar solutions. | a study has been made of the effect of urea upon the hydrolysis of 2-naphthyl acetate (2-na) catalyzed by lipase from rhizopus arrhizus in aot-heptane-water reverse micellar solutions at ph 7. the partition constants, k, of 2-na between n-heptane and aqueous urea solutions in the absence of micelles were also determined. it was found that k decreases when the concentration of urea increases. in aqueous solution the rate of hydrolysis of 2-na catalyzed by lipase is dependent on the concentration ... | 2005 | 15694427 |
| comparative mitochondrial genomics in zygomycetes: bacteria-like rnase p rnas, mobile elements and a close source of the group i intron invasion in angiosperms. | to generate data for comparative analyses of zygomycete mitochondrial gene expression, we sequenced mtdnas of three distantly related zygomycetes, rhizopus oryzae, mortierella verticillata and smittium culisetae. they all contain the standard fungal mitochondrial gene set, plus rnpb, the gene encoding the rna subunit of the mitochondrial rnase p (mtp-rna) and rps3, encoding ribosomal protein s3 (the latter lacking in r.oryzae). the mtp-rnas of r.oryzae and of additional zygomycete relatives have ... | 2005 | 15689432 |
| combination therapy with amphotericin b lipid complex and caspofungin acetate of disseminated zygomycosis in diabetic ketoacidotic mice. | we studied the combination of amphotericin b lipid complex (ablc) and caspofungin in mice with disseminated rhizopus oryzae. combination therapy improved survival compared to that of mice given monotherapy and that of untreated controls (p < 0.05) but did not improve organ clearance. in addition, prophylactic combination therapy was not more effective than prophylactic ablc alone. | 2005 | 15673781 |
| caspofungin inhibits rhizopus oryzae 1,3-beta-d-glucan synthase, lowers burden in brain measured by quantitative pcr, and improves survival at a low but not a high dose during murine disseminated zygomycosis. | rhizopus oryzae is the most common cause of zygomycosis, a life-threatening infection that usually occurs in patients with diabetic ketoacidosis. despite standard therapy, the overall rate of mortality from zygomycosis remains >50%, and new strategies for treatment are urgently needed. the activities of caspofungin acetate (cas) and other echinocandins (antifungal inhibitors of the synthesis of 1,3-beta-d-glucan synthase [gs]) against the agents of zygomycosis have remained relatively unexplored ... | 2005 | 15673756 |
| rhizopus oryzae adheres to, is phagocytosed by, and damages endothelial cells in vitro. | rhizopus oryzae is the most common cause of zygomycosis, a life-threatening infection that usually occurs in immunocompromised patients. a characteristic hallmark of zygomycosis is angioinvasion by the fungus, resulting in thrombosis and subsequent tissue necrosis. interactions between r. oryzae and vascular endothelial cells are therefore likely of central importance to the organism's pathogenetic strategy. we studied the ability of r. oryzae to adhere to and damage human umbilical vein endothe ... | 2005 | 15664916 |
| reducing by-product formation in l-lactic acid fermentation by rhizopus oryzae. | during l-lactic acid fermentation by rhizopus oryzae, increasing the phosphate level in the fermentation medium from 0.1 g l(-1) to 0.6 g l(-1) kh2po4 reduced the maximal concentration of l-lactic acid and fumaric acid from 85 g l(-1) to 71 g l(-1) and from 1.36 g l(-1) to 0.18 g l(-1), respectively; and it decreased the fermentation time from 72 h to 52 h. phosphate at 0.40 g l(-1) kh2po4 was suitable for both minimizing fumaric acid accumulation and benefiting l-lactic acid production. | 2005 | 15654619 |
| synthesis of ethyl phenylacetate by lyophilized mycelium of aspergillus oryzae. | lyophilized mycelia of aspergillus oryzae cbs 102.07, aspergillus oryzae mim, rhizopus oryzae cbs 112.07, rhizopus oryzae cbs 391.34, rhizopus oryzae cbs 260.28 and rhizopus oryzae cbs 328.47 were tested in this study to select the best biocatalysts for ethanol acylation with phenylacetic acid. the mycelium-bound carboxylesterase activity of a. oryzae mim, which exhibited the best performances, was initially investigated at 50 degrees c, either in 0.1 m phosphate buffer or in n-heptane to cataly ... | 2005 | 15650849 |
| microbial transformation of tannin-rich substrate to gallic acid through co-culture method. | modified solid-state fermentation (mssf) of tannin-rich substrate yielding tannase and gallic acid was carried out using a co-culture of the filamentous fungi, rhizopus oryzae (ro iit rb-13, nrrl 21498) and aspergillus foetidus (gmrb013 mtcc 3557). powdered fruits of terminalia chebula and powdered pod cover of caesalpinia digyna was used in the process and the different process parameters for maximum production of tannase and gallic acid by co-culture method were optimized through media enginee ... | 2005 | 15627566 |
| inactivation of fungi in vitro by photosensitization: preliminary results. | photosensitization is based on the interaction of 2 completely non-toxic agents--a photosensitizer, accumulated in microorganisms, and visible light. this interaction induces radical-based cytotoxic reactions in the presence of oxygen. the photosensitization phenomenon is widely involved in the treatment of tumors in oncology, in curing arthritis and atherosclerosis. in this work, the possibility to inactivate pathogenic and harmful fungi by photosensitization is shown. a new treatment methodolo ... | 2004 | 15627327 |
| improved secretory production of glucoamylase in pichia pastoris by combination of genetic manipulations. | rhizopus oryzae glucoamylase (ga) has been genetically engineered with modified signal peptide (msp), increased copy number of the gene, and coexpression of sec4, a gene encoding a rab protein associated with secretory vesicles, and its secretion level has been successfully raised up to 100-fold in pichia pastoris. the msp was designed to contain the signal peptide of mouse salivary alpha-amylase (s8l) fused to the pro-region of the signal peptide of saccharomyces cerevisiae alpha-mating factor ... | 2005 | 15607743 |
| cutaneous zygomycosis due to rhizopus oryzae in a patient with acute lymphoblastic leukemia. | we report herein a case of primary cutaneous zygomycosis caused by rhizopus oryzae in a 7-year-old girl with acute lymphoblastic leukemia (all) receiving intensive chemotherapy. the diagnosis was based on observation of hyphal elements in cutaneous biopsy and isolation of the fungus in culture. the patient responded to surgical intervention and treatment with amphotericin b. | 2004 | 15601461 |
| paranasal sinus mycoses. | the incidence of paranasal sinus mycoses (fungal sinusitis) varies widely with higher frequency in sudan, southwestern states of usa and north india, which have hot and dry climate. the disease has been described as having four types: allergic, non-invasive, invasive and fulminate. a possible fifth type: non-invasive destructive may also exist. in a prospective study of 176 cases of fungal sinusitis from our centre, on the basis of clinical, radiological, histopathologic and mycologic findings t ... | 2000 | 15597677 |
| investigation of the importance of the c-2 and c-13 oxygen functions in the transformation of stemodin analogues by rhizopus oryzae atcc 11145. | incubation of 2alpha,13(r)-dihydroxystemodane (3) with rhizopus oryzae atcc 11145 gave 2alpha,7beta,13(r)-trihydroxystemodane (11) while biotransformation of 13(r)-hydroxystemodan-2-one (5) yielded 6alpha,13(r)-dihydroxystemodan-2-one (12) and 7beta,13(r)-dihydroxystemodan-2-one (13). bioconversion of 2beta,13(r)-dihydroxystemodane (7) with rhizopus afforded 2beta,7,13(r)-trihydroxystemodane (14). the results complement data from our previous work and provide more information about the effect of ... | 2004 | 15587705 |
| increased levels of policosanol and very long-chain fatty acids in potato pulp fermented with rhizopus oryzae. | significant amounts of policosanol and very long-chain fatty acids (vlfas) ranging in carbon length from 22 to 30 were found in the lipophilic fraction obtained from potato pulp fermented with rhizopus oryzae. it is believed that these compounds would have originally been present as suberin-related compounds, but not as wax, in the periderm of potato tubers and concentrated into potato pulp during the process of starch production. moreover, the policosanol and vlfas extracted from potato pulp wi ... | 2004 | 15564684 |
| molecular cloning of a gene encoding endo-beta-d-1,4-glucanase pce1 from phycomyces nitens. | we previously cloned three endoglucanase genes, rce1, rce2, and rce3, from rhizopus oryzae as the first cellulase genes from the subdivision zygomycota. in this study, an endoglucanase gene, designated a pce1 gene, was cloned by plaque hybridization with the codon usage-optimized rce1 gene as a probe from phycomyces nitens, a member of the subdivision zygomycota. the pec1 gene had an open reading frame of 1,038 nucleotides encoding an endoglucanase (pce1) of 346 amino acid residues. the amino ac ... | 2004 | 15564668 |
| interesterification activity of rhizopus delemar lipase in phospholipid microemulsions. | the interesterification of olive oil with palmitic acid catalyzed by rhizopus delemar lipase was investigated in phospholipid microemulsion systems. soybean lecithin was used as the amphiphilic component. the maximal reaction rate was obtained at a buffer ph of 5.5-6.0. the reaction rate was also dependent on the w(l) (= [h2o]/[lecithin]) value and attained a maximum at w(l)=5. the reaction rate reached a maximum at a palmitic acid concentration of 350 mm. the molar fraction of the interesterifi ... | 2004 | 15542321 |
| [invasive maxilar sinusitis by rhizopus oryzae]. | we herein present a diabetic with non hodgkin lymphoma patient that had been treated with steroids and developed fungal invasive sinusitis. the patient had intensive facial pain that did not respond to antibiotics and on clinical inspection had a necrotic lesion on right nasal area. a smear and biopsy tissue showed broad non septate hyphae and on cultures rhizopus oryzae was isolated. there was an unfavorable outcome, and the patient died even though liposomal amphotericin b was administered and ... | 1997 | 15538825 |
| microbial metabolism. part 5. dihydrokawain. | preparative scale fermentation of (6s)-dihydrokawain (1) with rhizopus arrhizus (atcc 11145) gave 3'-hydroxydihydrokawain (2) and (8s)-hydroxydihydrokawain (3). structure elucidation of the metabolites was based on spectroscopic data. the c-8 absolute configuration of (3) was assessed via its mosher's esters. | 2004 | 15516767 |
| rhinoorbital mucormycosis secondary to rhizopus oryzae: a case report and literature review. | mucormycosis is a form of fulminant invasive fungal infection of the sinonasal tract that often extends to the orbit, brain, palate, and skin. it is caused by members of the order mucorales, and it is considered to be the most fatal fungal infection known to man because it is rapidly disseminated by the blood vessels. it is most commonly associated with diabetic ketoacidosis, hematologic malignancies, acquired immunodeficiency syndrome, and immunosuppressive therapy. this rare opportunistic infe ... | 2004 | 15487636 |
| [cloning and heterologous expression of a novel delta6 -desaturase gene from rhizopus arrhizus nk030037]. | a 593 bp dna fragment was amplified from rhizopus arrhizus nk030037 with degenerate oligonucleotide primers designed based on the sequences information for fungi delta6-fatty acid desaturase genes by rt-pcr and sequenced. gene specific primers derived from this partial sequence were used for the amplification of the 3'- and 5'-ends of this cdna by race method, and this lead to a full-length cdna sequence of 1 482 bp was amplified. sequence analysis showed this cdna sequence had an open reading f ... | 2004 | 15473328 |
| identification and characterization of a novel delta12-fatty acid desaturase gene from rhizopus arrhizus. | based on the sequence information of delta12-fatty acid desaturase genes (from mucor circinelloides, mortierella alpina, mucor rouxii and aspergillus nidulans), which were involved in the conversion from c18:1 to c18:2, a cdna sequence putatively encoding a delta12-fatty acid desaturase was isolated from rhizopus arrhizus using the combination of reverse transcription-polymerase chain reaction (rt-pcr) and rapid amplification of cdna ends (race) methods. sequence analysis indicated that it had a ... | 2004 | 15327973 |
| optimization of l-lactic acid production from glucose by rhizopus oryzae atcc 52311. | the effect of nutrients on l(+)-lactic acid production from glucose was investigated using rhizopus oryzae atcc 52311. from the shake-flask experiments, the optimal medium composition was defined for improved lactic-acid production. in order to enhance lactic-acid production rate and product yield, controlled aeration in a bubble column was conducted under optimal conditions. results showed a maximum lactic-acid production rate of 2.58 g/l/h was obtained with an initial glucose concentration of ... | 1999 | 15304710 |
| direct production of ethanol from raw corn starch via fermentation by use of a novel surface-engineered yeast strain codisplaying glucoamylase and alpha-amylase. | direct and efficient production of ethanol by fermentation from raw corn starch was achieved by using the yeast saccharomyces cerevisiae codisplaying rhizopus oryzae glucoamylase and streptococcus bovis alpha-amylase by using the c-terminal-half region of alpha-agglutinin and the flocculation functional domain of flo1p as the respective anchor proteins. in 72-h fermentation, this strain produced 61.8 g of ethanol/liter, with 86.5% of theoretical yield from raw corn starch. | 2004 | 15294847 |
| model studies on the formation of monochloropropanediols in the presence of lipase. | the formation of chloropropanols was investigated using model systems comprised of lipase, vegetable oil or fat, water, and sodium chloride. the results showed that measurable levels of the foodborne carcinogen 3-chloro-1,2-propanediol (3-mcpd) are formed in the presence of commercially available lipases of mammalian, vegetable, and fungal origins, incubated at temperatures of 40 degrees c. the highest yield of 3-mcpd was obtained in reaction mixtures containing lipase from rhizopus oryzae, and ... | 2004 | 15291482 |
| a cholesterol biosynthesis inhibitor from rhizopus oryzae. | a bile acid derivative, methyl cholate (1), was isolated from etoac extract of the fungus rhizopus oryzae as a cholesterol biosynthesis inhibitor. it showed moderate inhibitory activity on cholesterol biosynthesis in human chang liver cells. compound 1 exhibited inhibitory effect on the later step of cholesterol biosynthesis, indicating that its action mode is different from that of statins that act on the hmg-coa reductase. | 2004 | 15283464 |
| genetic diversity in rhizopus oryzae strains as revealed by the sequence of lactate dehydrogenase genes. | twenty-seven strains of rhizopus oryzae accumulating predominantly lactic acid were shown to possess two ldh genes, ldha and ldhb, encoding nad-dependent lactate dehydrogenases. variation in nucleotide sequence was identified for each gene from different strains, and similar phylogenetic trees were obtained based on the nucleotide sequences of both genes. the other 21 strains of r. oryzae accumulating predominantly fumaric and malic acids contained a single orf of ldhb. compared to the strains a ... | 2004 | 15278242 |
| use of immobilized lipases for lipase purification via specific lipase-lipase interactions. | lipase from pseudomonas fluorescens (pfl), an enzyme with a great tendency to yield bimolecular aggregates, was immobilized via multipoint covalent attachment on glyoxyl-agarose in the presence of triton x-100. this strategy permitted to obtain the enzyme with the active center oriented towards the reaction medium. this immobilized enzyme presents the capacity of specifically adsorbing pfl molecules, that can be easily desorbed by the use of detergents. more interesting, the enzyme was also able ... | 2004 | 15233541 |
| purification of the extracellular pectinolytic enzyme from the fungus rhizopus oryzae nbrc 4707. | the pectinolytic enzyme from the solid-state culture of rhizopus oryzae nbrc 4707 was purified to homogeneity by column chromatography on cm-toyopearl 650 m and hydroxylapatite. the molecular weight of the enzyme was estimated by sds-polyacrylamide gel electrophoresis to be 31,000 and was reduced to 29,700 after treatment with endoglycosidase h. maximal activity was observed near ph 4.5 at 45 degrees c. the enzyme was shown to be endopolygalacturonase, as judged from the formation of oligogalact ... | 2004 | 15160610 |
| cloning and functional characterization of the rhizopus oryzae high affinity iron permease (rftr1) gene. | rhizopus oryzae is the most common etiologic agent of mucormycosis. clinical and animal model data clearly demonstrate that the presence of elevated available serum iron predisposes the host to develop mucormycosis. therefore, the high affinity iron permease (rftr1) which encodes a protein required to scavenge iron from the environment, is highly likely to be a critical determinant of virulence for r. oryzae. we have cloned rftr1 by using a pcr approach relying on degenerate primers designed fro ... | 2004 | 15158278 |
| complete resolution of pulmonary rhizopus oryzae infection with itraconazole treatment: more evidence of the utility of azoles for zygomycosis. | zygomycosis often requires aggressive surgical and antifungal therapy. we report a non-neutropenic patient with myelodysplastic syndrome and iron overload receiving cytotoxic therapy who presented with pulmonary rhizopus oryzae infection. this patient was cured through the use of itraconazole alone and the literature on the utility of azole antifungals for zygomycosis is reviewed. | 2004 | 15078434 |
| development of a system for integrative and stable transformation of the zygomycete rhizopus oryzae by agrobacterium-mediated dna transfer. | two transformation systems, based on the use of cacl(2)/peg and agrobacterium tumefaciens, respectively, were developed for the zygomycete rhizopus oryzae. irrespective of the selection marker used, a pyr4 marker derived from r. niveus or a dominant amds(+) marker from aspergillus nidulans, and irrespective of the configuration of the transforming dna (linear or circular), the transformants obtained with the cacl(2)/peg transformation method were found to carry multiple copies of tandemly linked ... | 2004 | 15067540 |
| expression of a rhizopus oryzae lipase in pichia pastoris under control of the nitrogen source-regulated formaldehyde dehydrogenase promoter. | a rhizopus oryzae lipase gene has been expressed in pichia pastoris as a reporter using the formaldehyde dehydrogenase 1 promoter (pfld1) of this organism, which has been reported to be strongly and independently induced by either methanol as sole carbon source or methylamine as sole nitrogen source. levels of lipase expressed and secreted under the control of the pfld1 at different induction conditions have been compared to those obtained with the commonly used alcohol oxidase 1 promoter (paox1 ... | 2004 | 15063618 |
| investigation of the importance of the c-2 oxygen function in the transformation of stemodin analogues by rhizopus oryzae atcc 11145. | a new stemodinoside, stemodin-alpha-l-arabinofuranoside (5), was isolated from the plant stemodia maritima. incubation of stemodin (2) with rhizopus oryzae atcc 11145 gave 2 alpha,7 beta,13(s)-trihydroxystemodane (17) and 2 alpha,3 beta,13(s),16 alpha-tetrahydroxystemodane (18) whilst stemodinone (8) afforded 6 alpha,13(s)-dihydroxystemodan-2-one (19). the bioconversion of 2 beta,13(s)-dihydroxystemodane (10) by the fungus yielded 2 beta,7 beta,13(s)-trihydroxystemodane (20) whereas stemod-12-en ... | 2004 | 15016566 |
| repair of plasmid dna used for transformation of rhizopus oryzae by gene conversion. | techniques for genetic manipulation of the filamentous fungus rhizopus have been hampered due to a lack of understanding regarding the recombination and replication mechanisms that affect the fate of introduced dna. the ability to target chromosomal integration of a plasmid has been difficult because dna transformed into rhizopus rarely integrates and is autonomously replicated in a high molecular weight concatenated arrangement (i.e., series or chain). linearization of the plasmid prior to tran ... | 2004 | 15007626 |
| evidence for selective hydrolysis of aliphatic copolyesters induced by lipase catalysis. | high molar mass random poly(butylene succinate-co-butylene sebacate), p(bs-co-bse), and poly(butylene succinate-co-butylene adipate), p(bs-co-ba), with different composition, were synthesized and subjected to enzymatic hydrolysis by lipase from mucor miehei or from rhizopus arrhizus. the enzymatic hydrolysis of p(bs-co-bse)s and p(bs-co-ba)s films produced a mixture of water-soluble monomers and co-oligomers that were separated and identified by on-line high performance liquid chromatography/ele ... | 2004 | 15003003 |
| bioconversion of waste office paper to l(+)-lactic acid by the filamentous fungus rhizopus oryzae. | l(+)-lactic acid production was investigated using an enzymatic hydrolysate of waste office automation (oa) paper in a culture of the filamentous fungus rhizopus oryzae. in 4 d culture, 82.8 g/l glucose, 7 g/l xylose, and 3.4 g/l cellobiose contained in the hydrolysate were consumed to produce 49.1 g/l of lactic acid. the lactic acid yield and production rate were only 0.59 g/g and 16.3 g/l/d, respectively, only 75% and 61% of the results from the glucose medium. the low production rate from was ... | 2004 | 14987724 |
| biosynthesis of tannase and gallic acid from tannin rich substrates by rhizopus oryzae and aspergillus foetidus. | modified solid-state fermentation (mssf) of tannin-rich substrates for production of tannase and gallic acid was carried out using two fungal cultures, rhizopus oryzae (ro iit rb-13, nrrl 21498) and aspergillus foetidus (gmrb013 mtcc 3557). the tannin rich substrates included powdered fruits of terminalia chebula and caesalpinia digyna pod cover powder. the different environmental parameters for the maximum production of tannase and gallic acid were optimized through media engineering. the highe ... | 2004 | 14768027 |
| rhizopus arrhizus--a producer for simultaneous saccharification and fermentation of starch waste materials to l(+)-lactic acid. | rhizopus arrhizus, strain dar 36017, produced l(+)-lactic acid in a simultaneous saccharification and fermentation process using starch waste effluents. lactic acid at 19.5-44.3 g l(-1) with a yield of 0.85-0.96 g g(-1) was produced in 40 h using 20-60 g starch l(-1). supplementation of nitrogen source may be unnecessary if potato or corn starch waste effluent was used as a production medium. | 2003 | 14719810 |
| identification and characterization of a novel delta6-fatty acid desaturase gene from rhizopus arrhizus. | a cdna sequence putatively encoding a delta(6)-fatty acid desaturase was isolated from rhizopus arrhizus using reverse transcription polymerase chain reaction and rapid amplification of cdna ends methods. sequence analysis indicated that this cdna sequence had an open reading frame of 1377 bp encoding 458 amino acids of 52 kda. the deduced amino acid sequence showed high similarity to those of fungal delta(6)-fatty acid desaturases which comprised the characteristics of membrane-bound desaturase ... | 2004 | 14706830 |
| enantioselective transesterification using lipase-displaying yeast whole-cell biocatalyst. | an enantioselective transesterification in non-aqueous organic solvent was developed by utilizing a lipase-displaying yeast whole cell biocatalyst constructed in our previous study. as a model reaction, optical resolution of (rs)-1-phenylethanol, which serves as one of chiral building blocks, was carried out by enantioselective transesterification with vinyl acetate. recombinant rhizopus oryzae lipase displayed on the yeast cell surface retained its activity in hexane, heptane, cyclohexane and o ... | 2004 | 14689244 |
| effect of fungi fermentation on organoleptic properties, energy content and in-vitro multienzyme digestibility of cassava products (flour & gari). | the present study sought to investigate the effect of fungi fermentation on the energy content, sensory quality and the digestibility (in vitro) of cassava products (flour and gari). the fungi fermented cassava products (gari and flour) were produced, by fermenting cassava mash with pure strains of some common saprophytes, namely, aspergillus flavus, aspergillus niger, rhizopus oryzae and saccharomyces spp (baker's yeast and palm wine yeast) for 72 hrs before processing into cassava flour and ga ... | 2003 | 14653508 |