Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| congenital cytomegalovirus and zika infections. | congenital infections affecting newborn infants can have potentially devastating clinical outcomes. they are usually caused by viruses that infect mothers during pregnancy and are transmitted to the fetus or newborn during the prenatal, perinatal or postnatal periods. congenital cytomegalovirus (ccmv) is the most common congenital infection affecting up to 2.5% of all live births. even though most infected infants are asymptomatic at birth, ccmv is an important cause of neurodevelopmental impair ... | 2020 | 32281058 |
| vector competence of virginia mosquitoes for zika and cache valley viruses. | vector-borne diseases are a major public health concern and cause significant morbidity and mortality. zika virus (zikv) is the etiologic agent of a massive outbreak in the americas that originated in brazil in 2015 and shows a strong association with congenital zikv syndrome in newborns. cache valley virus (cvv) is a bunyavirus that causes mild to severe illness in humans and ruminants. in this study, we investigated the vector competence of virginia mosquitoes for zikv and cvv to explore their ... | 2020 | 32276649 |
| 2', 5'-oligoadenylate synthetase 2 (oas2) inhibits zika virus replication through activation of type ι ifn signaling pathway. | 2', 5'-oligoadenylate synthetase 2 (oas2) has been known as an antiviral interferon-stimulated gene (isg). however, the role of oas2 on zika virus (zikv) replication is still unknown. in this study, we sought to explore the effect of oas2 on zikv replication and its underlying mechanism. | 2020 | 32276512 |
| flavivirus nonstructural protein ns5 dysregulates hsp90 to broadly inhibit jak/stat signaling. | pathogenic flaviviruses antagonize host cell janus kinase/signal transducer and activator of transcription (jak/stat) signaling downstream of interferons α/β. here, we show that flaviviruses inhibit jak/stat signaling induced by a wide range of cytokines beyond interferon, including interleukins. this broad inhibition was mapped to viral nonstructural protein 5 (ns5) binding to cellular heat shock protein 90 (hsp90), resulting in reduced janus kinase-hsp90 interaction and thus destabilization of ... | 2020 | 32272626 |
| immunogenicity and efficacy of a recombinant human adenovirus type 5 vaccine against zika virus. | zika virus (zikv) is a significant public health concern due to the pathogen's ability to be transmitted by either mosquito bite or sexual transmission, allowing spread to occur throughout the world. the potential consequences of zikv infection to human health, specifically neonates, necessitates the development of a safe and effective zika virus vaccine. here, we developed an intranasal zika vaccine based upon the replication-deficient human adenovirus serotype 5 (had5) expressing zikv pre-memb ... | 2020 | 32272595 |
| structure-activity relationship of flavonoid bifunctional inhibitors against zika virus infection. | zika virus (zikv) infection is a global public health problem due to its rapid spread and the possibility of causing microcephaly. currently, no specific antivirals against zikv are available for treatment. in the present study, several flavonoids (galangin, kaempferide, quercetin, myricetin and egcg) were found to reduce zikv induced plaques and viral rna copies with negligible cytotoxic effects on host cells. in addition, inhibition of zikv propagation by flavonoids showed structure-activity r ... | 2020 | 32272109 |
| il-27 signaling activates skin cells to induce innate antiviral proteins and protects against zika virus infection. | in the skin, antiviral proteins and other immune molecules serve as the first line of innate antiviral defense. here, we identify and characterize the induction of cutaneous innate antiviral proteins in response to il-27 and its functional role during cutaneous defense against zika virus infection. transcriptional and phenotypic profiling of epidermal keratinocytes treated with il-27 demonstrated activation of antiviral proteins oas1, oas2, oasl, and mx1 in the skin of both mice and humans. il-2 ... | 2020 | 32270034 |
| comparative toxicity of larvicides and growth inhibitors on aedes aegypti from select areas in jamaica. | insecticide resistance has become problematic in tropical and subtropical regions, where aedes mosquitoes and aedes-borne arboviral diseases thrive. with the recent occurrence of chikungunya and the zika virus in jamaica, the ministry of health and wellness, jamaica, partnered with the united states agency for international development to implement multiple intervention activities to reduce the aedes aegypti populations in seven parishes across the island and to assess the susceptibility of coll ... | 2020 | 32269812 |
| [adequate solid waste management as a protection factor against dengue casesgestión adecuada de los residuos sólidos como factor de protección contra los casos de dengue]. | to investigate whether solid waste management and municipal socioeconomic indicators are associated with incidence rates of dengue, zika, and chikungunya in municipalities located in the state of minas gerais, brazil. | 2020 | 32269592 |
| two is better than one: evidence for t-cell cross-protection between dengue and zika and implications on vaccine design. | dengue virus (denv, family flaviviridae, genus flavivirus) exists as four distinct serotypes. generally, immunity after infection with one serotype is protective and lifelong, though exceptions have been described. however, secondary infection with a different serotype can result in more severe disease for a minority of patients. host responses to the first denv infection involve the development of both cross-reactive antibody and t cell responses, which, depending upon their precise balance, ma ... | 2020 | 32269575 |
| the aedes aegypti domino ortholog p400 regulates antiviral exogenous small interfering rna pathway activity and ago-2 expression. | arboviruses are pathogens of humans and animals. a better understanding of the interactions between these pathogens and the arthropod vectors, such as mosquitoes, that transmit them is necessary to develop novel control measures. a major antiviral pathway in the mosquito vector is the exogenous small interfering rna (exo-sirna) pathway, which is induced by arbovirus-derived double-stranded rna in infected cells. although recent work has shown the key role played by argonaute-2 (ago-2) and dicer- ... | 2020 | 32269152 |
| exploring evolutionary constraints in the proteomes of zika, dengue, and other flaviviruses to find fitness-critical sites. | dengue virus (denv) challenges vaccine design due to antibody-dependent enhancement (ade) and evidence suggests that zika virus (zikv) experiences ade with denv and west nile virus (wnv) antibodies. thus, multiple flaviviruses must be considered when developing novel therapies against zikv. we analyzed 42 flavivirus polyproteins in their evolutionary context to identify motifs conserved in sequence with low real-time and evolutionary conformational flexibility, thought to be fitness-critical sit ... | 2020 | 32266427 |
| defeat dengue and zika viruses with a one-two punch of vaccine and vector blockade. | dengue virus (denv) and zika virus (zikv) are two mosquito-borne flaviviruses afflicting nearly half of the world population. human infection by these viruses can either be asymptomatic or manifest as clinical diseases from mild to severe. despite more cases are presented as self-limiting febrile illness, severe dengue disease can be manifested as hemorrhagic fever and hemorrhagic shock syndrome, and zikv infection has been linked to increased incidence of peripheral neuropathy guillain-barre sy ... | 2020 | 32265852 |
| enhanced surveillance and response approaches for pilgrims and local saudi populations against emerging nipah, zika and ebola viral diseases outbreaks threats. | increasing emergence and spread of nipah, zikv and ebola case and potential outbreaks threats have been reported in several regions around the globe. yet, emerging nipah, ebola and zika viral diseases outbreaks have been indirectly linked to substantially globalization of trade and travel, climate change and intense urbanization impact, healthcare and socioeconomic inequities as well in affected community settings. although no case has been documented in saudi arabia, there is a great risk of su ... | 2020 | 32265162 |
| insights into structures and dynamics of flavivirus proteases from nmr studies. | nuclear magnetic resonance (nmr) spectroscopy plays important roles in structural biology and drug discovery, as it is a powerful tool to understand protein structures, dynamics, and ligand binding under physiological conditions. the protease of flaviviruses is an attractive target for developing antivirals because it is essential for the maturation of viral proteins. high-resolution structures of the proteases in the absence and presence of ligands/inhibitors were determined using x-ray crystal ... | 2020 | 32260545 |
| using next generation sequencing to study the genetic diversity of candidate live attenuated zika vaccines. | zika virus (zikv) is a mosquito-transmitted positive-sense rna virus in the family flaviviridae. candidate live-attenuated vaccine (lav) viruses with engineered deletions in the 3' untranslated region (utr) provide immunity and protection in animal models of zikv infection, and phenotypic studies show that lavs retain protective abilities following in vitro passage. the present study investigated the genetic diversity of wild-type (wt) parent zikv and its candidate lavs using next generation seq ... | 2020 | 32260110 |
| emergence of zika virus. | zika virus (zikv) is an arthropod-borne virus (arbovirus) of the family flaviviridae, genus flavivirus, transmitted by the bite of infected mosquitoes. zikv was first isolated from a non-human primate in 1947, zikv infections in humans were sporadic during 60 years before emerging in the pacific in 2007 and 2013 and in brazil in 2015. due to a non-specific clinical presentation, zika fever can be misdiagnosed with other arboviruses such as dengue and chikungunya. zikv infections were associated ... | 2015 | 32260029 |
| emerging and neglected zoonoses in transplant population. | zoonoses represent a problem of rising importance in the transplant population. a close relationship and changes between human, animal and environmental health ("one health" concept) significantly influence the transmission and distribution of zoonotic diseases. the aim of this manuscript is to perform a narrative review of the published literature on emerging and neglected zoonoses in the transplant population. many reports on donor-derived or naturally acquired (re-)emerging arboviral infectio ... | 2020 | 32257849 |
| knowledge regarding zika virus infection among healthcare providers in an academic tertiary care center in riyadh, saudi arabia: a cross-sectional survey study. | zika virus (zikv) has become a major concern across the world. it is highly necessary for healthcare providers (hcps) to have sufficient knowledge about such a disease. the purpose of this study is to assess the knowledge regarding zikv among hcps in riyadh, saudi arabia. materials and methods. a cross-sectional survey study was conducted at a tertiary care center in riyadh, ksa, during a two-month period from september 19, 2016 to november 19, 2016. descriptive statistics were performed on data ... | 2020 | 32256906 |
| public perception about the zika virus in working professionals: a qualitative inquiry. | the global outbreak alert and response network recently warned that the zika virus-containing mosquitoes are being found in pakistan and the health sector is on high alert. it is thus significant to understand public beliefs and perceptions of the zika virus and vaccination in the current scenario of pakistan. | 2020 | 32249680 |
| homologous prime-boost with zika virus envelope protein and poly (i:c) induces robust specific humoral and cellular immune responses. | the recent outbreaks of zika virus (zikv) infection and the potential association with guillain-barré syndrome in adults and with congenital abnormalities have highlighted the urgency for an effective vaccine. the zikv envelope glycoprotein (ezikv) is the most abundant protein on the virus surface, and has been evaluated together with the pre-membrane protein (prm) of the viral coat as a vaccine candidate in clinical trials. in this study, we performed a head-to-head comparison of the immune res ... | 2020 | 32247567 |
| congenital infections in hong kong: an overview of torch. | congenital infections refer to a group of perinatal infections that may have similar clinical presentations, including rash and ocular findings. torch is the acronym that covers these infections (toxoplasmosis, other [syphilis], rubella, cytomegalovirus, herpes simplex virus). there are, however, other important causes of intrauterine/perinatal infections, including enteroviruses, varicella zoster virus, zika virus, and parvovirus b19. intrauterine and perinatal infections are significant causes ... | 2020 | 32245914 |
| quantitative and selective surface plasmon resonance response based on a reduced graphene oxide-polyamidoamine nanocomposite for detection of dengue virus e-proteins. | dengue viral infection is one of the most common deadliest diseases and has become a recurrent issue for public health in tropical countries. although the spectrum of clinical diagnosis and treatment have recently been established, the efficient and rapid detection of dengue virus (denv) during viremia and the early febrile phase is still a great challenge. in this study, a dithiobis (succinimidyl undecanoate, dsu)/amine-functionalized reduced graphene oxide--polyamidoamine dendrimer (dsu/amine- ... | 2020 | 32245185 |
| a public health systems view of risk communication about zika. | the spread of zika virus throughout latin america and parts of the united states in 2016 and 2017 presented a challenge to public health communicators. the objective of our study was to describe emergency risk communication practices during the 2016-2017 zika outbreak to inform future infectious disease communication efforts. | 2020 | 32243762 |
| serologic surveillance of maternal zika infection in a prospective cohort in leon, nicaragua during the peak of the zika epidemic. | zika virus caused thousands of congenital anomalies during a recent epidemic. because zika emerged in areas endemic for dengue and these related flaviviruses elicit cross-reactive antibodies, it is challenging to serologically monitor pregnant women for zika infection. | 2020 | 32243482 |
| zika virus circulates at low levels in western and coastal kenya. | zika virus (zikv) was discovered over 70 years ago in east africa, but little is known about its circulation and pathogenesis there. | 2020 | 32242626 |
| role of physical therapy in a triage center during the zika virus epidemic. | 2020 | 32239166 | |
| a rapid and simple quantitative method for specific detection of smaller coterminal rna by pcr (desco-pcr): application to the detection of viral subgenomic rnas. | rnas that are 5'-truncated versions of a longer rna but share the same 3' terminus can be generated by alternative promoters in transcription of cellular mrnas or by replicating rna viruses. these truncated rnas cannot be distinguished from the longer rna by a simple two-primer rt-pcr because primers that anneal to the cdna from the smaller rna also anneal to-and amplify-the longer rna-derived cdna. thus, laborious methods, such as northern blot hybridization, are used to distinguish shorter fro ... | 2020 | 32238481 |
| experimental infection and vector competence of zika virus in aedes aegypti mosquitoes from acapulco, guerrero, mexico. | to gain a better understanding of the zika virus (zikv) vector transmission in mexico, we determined the vector competence of a local population of ae. aegypti (acapulco, guerrero) for a strain of zikv isolated from a mexican febrile patient. | 2020 | 32237563 |
| antiviral activity of benzavir-2 against emerging flaviviruses. | most flaviviruses are arthropod-borne viruses, transmitted by either ticks or mosquitoes, and cause morbidity and mortality worldwide. they are endemic in many countries and have recently emerged in new regions, such as the zika virus (zikv) in south-and central america, the west nile virus (wnv) in north america, and the yellow fever virus (yfv) in brazil and many african countries, highlighting the need for preparedness. currently, there are no antiviral drugs available to treat flavivirus inf ... | 2020 | 32235763 |
| investigation of the immunogenicity of zika glycan loop. | zika virus (zikv) is a major human pathogen and member of the flavivirus genus. previous studies have identified neutralizing antibodies from zika patients that bind to quaternary epitopes across neighboring envelope (e) proteins, called e dimer epitopes (ede). an asparagine-linked glycan on the "glycan loop" (gl) of the zikv envelope protein protects the functionally important "fusion loop" on the opposite e subunit in the dimer, and ede antibodies have been shown to bind to both of these loops ... | 2020 | 32234060 |
| evolutions and upcoming on zika virus diagnosis through an outbreak: a systematic review. | zika virus (zikv) is an enveloped, positive single-stranded sense rna virus transmitted by aedes species. many efforts have been conducted to find a good, reliable and cost-effective test for zikv diagnosis. diagnosis is still imprecise, expensive and there is not a standard model. we investigated the publications on zikv diagnostics and analyzed varieties of diagnostic methods, sensibility, specificity, and the evolution of new methodologies. conducted in accordance with the prisma-p statement, ... | 2020 | 32232942 |
| integrated arbovirus surveillance improves the detection onset of zika virus in panama. | we tested 700 serum samples collected throughout panama from 2015 to 2016 for detecting antibodies and rna of arboviruses. in convalescent specimens, microsphere immunoassay detected an antibody prevalence of 59.3% for dengue virus (denv) and 30.3% for zika virus (zikv), which included samples that were collected before the panamanian surveillance system reported the first case of zika in the country. for acute sera, the most common arbovirus was denv with 18 positive samples (6%), followed by f ... | 2020 | 32228787 |
| functional outcomes among a cohort of children in northeastern brazil meeting criteria for follow-up of congenital zika virus infection. | following the large outbreak of zika virus in the western hemisphere, many infants have been born with congenital zika virus infection. it is important to describe the functional outcomes seen with congenital infections to allow for their recognition and appropriate interventions. we evaluated 120 children conceived during the 2015-2016 zika virus outbreak in paraíba, brazil, who were approximately 24 months old, to assess functional outcomes. all children met either anthropometric criteria or l ... | 2020 | 32228785 |
| entomological surveillance associated with human zika cases in miri sarawak, malaysia. | two confirmed human cases of zika virus (zikv) were reported in the district of miri, sarawak, in 2016. following that, a mosquito-based zikv surveillance study was conducted within 200-m radius from the case houses. mosquito surveillance was conducted using five different methods, that is, biogents sentinel mosquito (bg) sentinel trap, modified sticky ovitrap, resting catch, larval surveillance, and conventional ovitrap. a total of 527 and 390 mosquito samples were obtained from the case houses ... | 2020 | 32228777 |
| the politics of disease epidemics: a comparative analysis of the sars, zika, and ebola outbreaks. | over the past few decades, disease outbreaks have become increasingly frequent and widespread. the epicenters of these outbreaks have differed, and could be linked to different economic contexts. arguably, the responses to these outbreaks have been "political" and inherently burdensome to marginalized populations. key lessons can be learned from exploring the narratives about the different epidemics in varying income settings. based on a review of the published medical, social, and political lit ... | 2020 | 32226719 |
| safety, tumor reduction, and clinical impact of zika virus injection in dogs with advanced-stage brain tumors. | malignant brain tumors are among the most aggressive cancers with poor prognosis and no effective treatment. recently, we reported the oncolytic potential of zika virus infecting and destroying the human central nervous system (cns) tumors in vitro and in immunodeficient mice model. however, translating this approach to humans requires pre-clinical trials in another immunocompetent animal model. here, we analyzed the safety of brazilian zika virus (zikvbr) intrathecal injections in three dogs be ... | 2020 | 32220305 |
| vaccination with aedes aegypti agbr1 delays lethal mosquito-borne zika virus infection in mice. | zika virus (zikv) is transmitted primarily by aedes aegypti mosquitoes, resulting in asymptomatic infection, or acute illness with a fever and headache, or neurological complications, such as guillain-barre syndrome or fetal microcephaly. previously, we determined that agbr1, a mosquito salivary protein, induces inflammatory responses at the bite site, and that passive immunization with agbr1 antiserum influences mosquito-transmitted zikv infection. here, we show that the active immunization of ... | 2020 | 32218189 |
| structure-based screening and validation of bioactive compounds as zika virus methyltransferase (mtase) inhibitors through first-principle density functional theory, classical molecular simulation and qm/mm affinity estimation. | recent zika virus (zikv) outbreak and association with human diseases such as neurological disorders have raised global health concerns. however, in the absence of an approved anti-zikv drug has generated urgency for the drug development against zikv infection. here, structure-based virtual screening of 8589 bioactive compounds, screened at the substrate-binding site of zikv nonstructural 5 (ns5)-based structure n-terminal methyltransferase (mtase) domain followed by admet (absorption, distribut ... | 2020 | 32216596 |
| zika virus infection and guillain-barré syndrome in northeastern mexico: a case-control study. | beginning august 2017, we conducted a prospective case-control investigation in monterrey, mexico to assess the association between zika virus (zikv) and guillain-barré syndrome (gbs). | 2020 | 32214354 |
| characterization of zika virus endocytic pathways in human glioblastoma cells. | zika virus (zikv) infections can cause microcephaly and neurological disorders. however, the early infection events of zikv in neural cells remain to be characterized. here, by using a combination of pharmacological and molecular approaches and the human glioblastoma cell t98g as a model, we first observed that zikv infection was inhibited by chloroquine and nh4cl, indicating a requirement of low intracellular ph. we further showed that dynamin is required as the zikv entry was affected by the s ... | 2020 | 32210929 |
| a combination of two human monoclonal antibodies limits fetal damage by zika virus in macaques. | human infection by zika virus (zikv) during pregnancy can lead to vertical transmission and fetal aberrations, including microcephaly. prophylactic administration of antibodies can diminish or prevent zikv infection in animal models, but whether passive immunization can protect nonhuman primates and their fetuses during pregnancy has not been determined. z004 and z021 are neutralizing monoclonal antibodies to domain iii of the envelope (ediii) of zikv. together the two antibodies protect nonpreg ... | 2020 | 32209664 |
| zika virus and its implications on cord blood banking and transplantation. | umbilical cord blood is an important cellular therapy product used for hematopoietic stem cell transplantation, but the us food and drug administration guidance regarding donor screening to reduce the risk of zika transmission has decreased the number of licensed, eligible cord blood units available for transplantation. there is a crucial need for updated travel risk assessment for zika virus transmission, validated screening tests for zika virus in umbilical cord blood, and further research on ... | 2020 | 32208533 |
| cemeteries in miami-dade county, florida are important areas to be targeted in mosquito management and control efforts. | definable habitats at the neighborhood level provide a wide range of favorable habitats with optimal conditions and environmental resources for mosquito survival. problematic habitats for controlling mosquitoes in urban environments such as tire shops, bromeliad patches, and construction sites must be taken into consideration in the development of effective mosquito management and control in urban areas. cemeteries are often located in highly urbanized areas serving as a haven for populations of ... | 2020 | 32208462 |
| zika virus infection in pregnant women in french guiana: more precarious-more at risk. | a recent study in french guiana suggested that populations living in precarious neighborhoods were more at risk for chikungunya chikv than those living in more privileged areas. the objective of the present study was to test the hypothesis that zika virus (zikv) infection was more frequent in precarious pregnant women than in non-precarious pregnant women, as reflected by their health insurance status. | 2020 | 32208419 |
| different populations of aedes aegypti and aedes albopictus (diptera: culicidae) from central africa are susceptible to zika virus infection. | zika virus (zikv) is a flavivirus (flaviviridae) transmitted to humans mainly by the bite of an infected aedes mosquitoes. aedes aegypti is the primary epidemic vector of zikv and ae. albopictus, the secondary one. however, the epidemiological role of both aedes species in central africa where ae. albopictus was recently introduced is poorly characterized. field-collected strains of ae. aegypti and ae. albopictus from different ecological settings in central africa were experimentally infected w ... | 2020 | 32203510 |
| comparison of clinical presentation and out-comes of chikungunya and dengue virus infections in patients with acute undifferentiated febrile illness from the sindh region of pakistan. | arboviruses are a cause of acute febrile illness and outbreaks worldwide. recent outbreaks of chikungunya virus (chikv) in dengue endemic areas have alarmed clinicians as unique clinical features differentiating chikv from dengue virus (denv) are limited. this has complicated diagnostic efforts especially in resource limited countries where lab testing is not easily available. therefore, it is essential to analyse and compare clinical features of laboratory confirmed cases to assist clinicians i ... | 2020 | 32203509 |
| peptide arrays incubated with three collections of human sera from patients infected with mosquito-borne viruses. | background: global outbreaks caused by emerging or re-emerging arthropod-borne viruses (arboviruses) are becoming increasingly more common. these pathogens include the mosquito-borne viruses belonging to the flavivirus and alphavirus genera. these viruses often cause non-specific or asymptomatic infection, which can confound viral prevalence studies. in addition, many acute phase diagnostic tests rely on the detection of viral components such as rna or antigen. standard serological tests are oft ... | 2019 | 32201571 |
| neurological outcomes of congenital zika syndrome in toddlers and preschoolers: a case series. | congenital zika syndrome causes a spectrum of neurological symptoms with varying effects on function that require different therapeutic strategies. to date, this spectrum of effects and its clinical implications have not been completely described. we describe the neurological examination findings in toddlers and preschoolers, including predominant symptom complexes and comorbidities. | 2020 | 32199080 |
| the developmental spectrum of prenatal zika virus exposure. | 2020 | 32199079 | |
| an optimized purified inactivated zika vaccine provides sustained immunogenicity and protection in cynomolgus macaques. | the recent spread of zika virus (zikv) through the americas and caribbean and its devastating consequences for pregnant women and their babies have driven the search for a safe and efficacious zikv vaccine. among the vaccine candidates, a first-generation zikv purified inactivated vaccine (zpiv), adjuvanted with aluminum hydroxide, developed by the walter reed army institute of research (wrair), has elicited high seroconversion rates in participants in three phase-i clinical trials. in collabora ... | 2020 | 32194996 |
| assessment of contraceptive use in puerto rico during the 2016 zika virus outbreak. | the objectives of this analysis were to 1) estimate prevalence of contraceptive use among women at risk for unintended pregnancy and 2) identify correlates of contraceptive use among women with ongoing or potential need for contraceptive services in puerto rico during the 2016 zika virus (zikv) outbreak. | 2020 | 32194040 |
| primary and secondary dengue virus infections elicit similar memory b-cell responses, but breadth to other serotypes and cross-reactivity to zika virus is higher in secondary dengue. | the 4 antigenically distinct serotypes of dengue virus (denv) share extensive homology with each other and with the closely related zika flavivirus (zikv). the development of polyclonal memory b cells (mbcs) to the 4 denv serotypes and zikv during denv infection is not fully understood. | 2020 | 32193549 |
| maternal zika virus (zikv) infection following vaginal inoculation with zikv-infected semen in timed-pregnant olive baboons. | zika virus (zikv) infection is now firmly linked to congenital zika syndrome (czs), including fetal microcephaly. while aedes species of mosquito are the primary vector for zikv, sexual transmission of zikv is a significant route of infection. zikv has been documented in human, mouse, and nonhuman primate (nhp) semen. it is critical to establish nhp models of the vertical transfer of zikv that recapitulate human pathogenesis. we hypothesized that vaginal deposition of zikv-infected baboon semen ... | 2020 | 32188737 |
| impact of temperature on the extrinsic incubation period of zika virus in aedes aegypti. | since zika virus (zikv) emerged as a global human health threat, numerous studies have pointed to aedes aegypti as the primary vector due to its high competence and propensity to feed on humans. the majority of vector competence studies have been conducted between 26-28°c, but arboviral extrinsic incubation periods (eips), and therefore transmission efficiency, are known to be affected strongly by temperature. to better understand the relationship between zikv eips and temperature, we evaluated ... | 2020 | 32187187 |
| imported arbovirus infections in spain, 2009-2018. | to determine the epidemiologic and clinical characteristics of patients in spain with imported arbovirus infections, we analyzed 22,655 records from a collaborative network for january 2009-december 2018. among 861 arbovirus infections, 845 were monoinfections (456 [53%] dengue, 280 [32.5%] chikungunya, 109 [12.7%] zika) and 16 (1.8%) were co-infections. most patients were travelers (56.3%) or immigrants returning to spain after visiting friends or relatives (31.3%). median patient age was 37 ye ... | 2020 | 32186486 |
| vector-borne transmission of the zika virus asian genotype in europe. | three autochthonous cases of zika virus occurred in southern france in august 2019. diagnosis relied on serology and transcription-mediated amplification. attempts for virus isolation and zikv genome rt-pcr detection remained negative. since the index case was not identified, we addressed the issue of genotyping and geographical origin by performing hemi-nested pcr and sequencing in the pr gene. analysis of 16 genotype-specific single nucleotides polymorphisms identified the asian genotype and s ... | 2020 | 32182748 |
| topology, antiviral functional residues and mechanism of ifitm1. | interferon-inducible transmembrane proteins (ifitm1/2/3) have been reported to suppress the entry of a wide range of viruses. however, their antiviral functional residues and specific mechanisms are still unclear. here, we firstly resolved the topology of ifitm1 on the plasma membrane where n-terminus points into the cytoplasm and c-terminus resides extracellularly. further, krrk basic residues of ifitm1 locating at 62-67 of the conserved intracellular loop (cil) were found to play a key role in ... | 2020 | 32182730 |
| ginkgolic acid inhibits fusion of enveloped viruses. | ginkgolic acids (ga) are alkylphenol constituents of the leaves and fruits of ginkgo biloba. ga has shown pleiotropic effects in vitro, including: antitumor effects through inhibition of lipogenesis; decreased expression of invasion associated proteins through ampk activation; and potential rescue of amyloid-β (aβ) induced synaptic impairment. ga was also reported to have activity against escherichia coli and staphylococcus aureus. several mechanisms for this activity have been suggested includi ... | 2020 | 32179788 |
| congenital microcephaly unrelated to flavivirus exposure in coastal kenya. | background: zika virus (zikv) was first discovered in east africa in 1947. zikv has caused microcephaly in the americas, but it is not known whether zikv is a cause of microcephaly in east africa. methods: we used surveillance data from 11,061 live births at kilifi county hospital in coastal kenya between january 2012 and october 2016 to identify microcephaly cases and conducted a nested case-control study to determine risk factors for microcephaly. gestational age at birth was estimated based ... | 2019 | 32175480 |
| into the woods: changes in mosquito community composition and presence of key vectors at increasing distances from the urban edge in urban forest parks in manaus, brazil. | mosquito-borne zika virus (zikv) was recently introduced into the americas and now has the potential to spill back into a sylvatic cycle in the region, likely involving non-human primates and aedes, haemagogus, and sabethes species mosquitoes. we investigated potential routes of mosquito-borne virus exchange between urban and sylvatic transmission cycles by characterizing mosquito communities in three urban forest parks that receive heavy traffic from both humans and monkeys in manaus, brazil. p ... | 2020 | 32173316 |
| codelivery of improved immune complex and virus-like particle vaccines containing zika virus envelope domain iii synergistically enhances immunogenicity. | zika virus (zikv) reemergence poses a significant health threat especially due to its risks to fetal development, necessitating safe and effective vaccines that can protect pregnant women. zika envelope domain iii (ze3) has been identified as a safe and effective vaccine candidate, however it is poorly immunogenic. we previously showed that plant-made recombinant immune complex (ric) vaccines are a robust platform to improve the immunogenicity of weak antigens. in this study, we altered the anti ... | 2020 | 32173095 |
| dengue fever complicated by liver dysfunction due to possible co-infection with hepatitis e in a returning traveller from cuba. | dengue fever is a mosquito-borne infection that co-circulates with chikungunya and zika virus infection in many parts of the world. dengue virus (denv) is occasionally responsible for acute hepatitis and a few cases of acute hepatitis due to co-infection with denv and hepatitis e virus have been described in india. a 37-year-old cuban woman living in italy was admitted to our hospital with a presumed arboviral infection upon her return to italy short after a 15-day trip to her home-country to vi ... | 2020 | 32172268 |
| one-step rt-qpcr assay for zikv rna detection in aedes aegypti samples: a protocol to study infection and gene expression during zikv infection. | zika virus (zikv) is transmitted to humans during the bite of an infected mosquito. in a scenario of globalization and climate change, the frequency of outbreaks has and will increase in areas with competent vectors, revealing a need for continuous improvement of zikv detection tools in vector populations. a simple, rapid and sensitive assay for viral detection is quantitative reverse transcription polymerase chain reaction (qrt-pcr), yet oligos optimized for zikv detection in mammalian cells an ... | 2020 | 32171303 |
| experience in diagnosing congenital zika syndrome in brazilian children born to asymptomatic mothers. | typical symptoms of primary zika virus infection are not specific and share similarities with other arbovirus infections such as dengue fever and chikungunya. as acute infection can be asymptomatic in up to 73% of cases, infants with microcephaly represent a diagnostic challenge for pediatricians. we describe the frequency of congenital zika syndrome (czs) in brazilian children born to asymptomatic pregnant mothers and its differential diagnosis. | 2020 | 32165128 |
| hematologic values of jamaican fruit bats (artibeus jamaicensis) and the effects of isoflurane anesthesia. | jamaican fruit bats (artibeus jamaicensis) are used as an animal model for several viruses, including middle east respiratory syndrome virus, dengue virus, zika virus, and tacaribe virus. however, despite ongoing studies regarding these pathogens, little is known regarding the bats' normal physiology. in this study, phlebotomy of the propetagial (cephalic) vein was performed to establish baseline hematologic parameters in an apparently healthy, captive population of jamaican fruit bats. furtherm ... | 2020 | 32164795 |
| [zika fever immunodiagnostics: overview of test systems.] | the threat of rapid spread of zika virus beyond endemic regions has given rise to more research in field of epidemiology and clinic, as well as to the search for zika fiver new diagnostic and preventive tools. between 2013 and 2017 in russia 18 cases of infection transmission by travellers were reported. fever zika reference monitoring center in volgograd research antiplague institute (volgograd, russian federation) provides counseling and methodological assistance on laboratory diagnosis and mo ... | 2019 | 32163680 |
| first report of aedes albopictus infected by dengue and zika virus in a rural outbreak in brazil. | in brazil, dengue (denv) and zika (zikv) viruses are reported as being transmitted exclusively by aedes aegypti in urban settings. this study established the vectors and viruses involved in an arbovirus outbreak that occurred in 2019 in a rural area of espírito santo state, brazil. mosquitoes collected were morphologically identified, sorted in samples, and submitted to molecular analysis for arboviruses detection. phylogenetic reconstruction was performed for the viral sequence obtained. all 39 ... | 2020 | 32163449 |
| the global scientific research response to the public health emergency of zika virus infection. | science studies have been a field of research for different knowledge areas, and they have been successfully used to analyse the construction of scientific knowledge, practice and dissemination. in this study, we aimed to verify how the zika epidemic has moulded the scientific articles published worldwide by analysing international collaborations and the knowledge landscape through time, as well as research topics and country involvement. | 2020 | 32163439 |
| the cyanobacterial saxitoxin exacerbates neural cell death and brain malformations induced by zika virus. | the northeast (ne) region of brazil commonly goes through drought periods, which favor cyanobacterial blooms, capable of producing neurotoxins with implications for human and animal health. the most severe dry spell in the history of brazil occurred between 2012 and 2016. coincidently, the highest incidence of microcephaly associated with the zika virus (zikv) outbreak took place in the ne region of brazil during the same years. in this work, we tested the hypothesis that saxitoxin (stx), a neur ... | 2020 | 32163415 |
| the impact of news exposure on collective attention in the united states during the 2016 zika epidemic. | in recent years, many studies have drawn attention to the important role of collective awareness and human behaviour during epidemic outbreaks. a number of modelling efforts have investigated the interaction between the disease transmission dynamics and human behaviour change mediated by news coverage and by information spreading in the population. yet, given the scarcity of data on public awareness during an epidemic, few studies have relied on empirical data. here, we use fine-grained, geo-ref ... | 2020 | 32163409 |
| computational methodology for peptide vaccine design for zika virus: a bioinformatics approach. | with the increasing frequency of viral epidemics, vaccines to augment the human immune response system have been the medium of choice to combat viral infections. the tragic consequences of the zika virus pandemic in south and central america a few years ago brought the issues into sharper focus. while traditional vaccine development is time-consuming and expensive, recent advances in information technology, immunoinformatics, genetics, bioinformatics, and related sciences have opened the doors t ... | 2020 | 32162248 |
| flavivirus envelope protein glycosylation: impacts on viral infection and pathogenesis. | flaviviruses encode one, two, or no n-linked glycosylation sites on their envelope proteins. glycosylation can impact virus interactions with cell surface attachment factors and also may impact virion stability and virus replication. envelope protein glycosylation has been identified as a virulence determinant for multiple flaviviruses, but the mechanisms by which glycosylation mediates pathogenesis remain unclear. in this gem, we summarize current knowledge on flavivirus envelope protein glycos ... | 2020 | 32161171 |
| climate variability, vulnerability, and natural disasters: a case study of zika virus in manabi, ecuador following the 2016 earthquake. | climate change presents complex and wide-reaching threats to human health. a variable and changing climate can amplify and unmask ecological and socio-political weaknesses and increase the risk of adverse health outcomes in socially vulnerable regions. when natural disasters occur in such areas, underlying climatic conditions may amplify the public health crisis. we describe an emerging epidemic of zika virus (zikv) in ecuador following the 2016 earthquake, which coincided with an exceptionally ... | 2017 | 32158994 |
| a review on zika virus outbreak, epidemiology, transmission and infection dynamics. | zika virus (zikv) is a newly emergent relative of the flaviviridae family and linked to dengue (denv) and chikungunya (chivkv). zikv is one of the rising pathogens promptly surpassing geographical borders. zikv infection was characterized by mild disease with fever, headache, rash, arthralgia and conjunctivitis, with exceptional reports of an association with guillain-barre syndrome (gbs) and microcephaly. however, since the end of 2015, an increase in the number of gbs associated cases and an a ... | 2020 | 32158705 |
| harnessing dengue rapid diagnostic tests for the combined surveillance of dengue, zika, and chikungunya viruses in laos. | recent expansions of vector-borne diseases highlight the need for improved surveillance, especially in resource-poor settings. dengue virus (denv), chikungunya virus (chikv), and zika virus (zikv) share the same vectors as well as similar clinical presentations, suggesting that combined surveillance would be useful. we hypothesized that blood spotted on dengue rapid diagnostic tests (rdts) could be harnessed for sample collection in remote areas for subsequent detection of denv, chikv, and zikv ... | 2020 | 32157991 |
| experimental infection of mid-gestation pregnant female and intact male sheep with zika virus. | zika virus (zikv) is an arbovirus that causes birth defects, persistent male infection, and sexual transmission in humans. the purpose of this study was to continue the development of an ovine zikv infection model; thus, two experiments were undertaken. in the first experiment, we built on previous pregnant sheep experiments by developing a mid-gestation model of zikv infection. four pregnant sheep were challenged with zikv at 57-64 days gestation; two animals served as controls. after 13-15 day ... | 2020 | 32156037 |
| the mechanism of the zika virus crossing the placental barrier and the blood-brain barrier. | zika virus (zikv) infection causes severe neurological symptoms in adults and fetal microcephaly and the virus is detected in the brain of microcephaly and meningoencephalitis patient. however, the mechanism of zikv crossing the physiological barrier to the central nervous systems (cns) remains elusive. the placental barrier and the blood brain barrier (bbb) protect the fetus from pathogens and ensure healthy brain development during pregnancy. in this study, we used human placenta trophoblasts ... | 2020 | 32153526 |
| adequate placental sampling for the diagnosis and characterization of placental infection by zika virus. | the detection of zika virus (zikv) in immunoprivileged anatomical sites, potential sites for viral persistence, may guide the confirmation of undefined cases of zikv infection and also bring to light unknown pathways of viral transmission. thus, this study aimed to characterize zikv infection in stratified, standardized placental samples in women with exanthematic febrile manifestations during pregnancy and compare findings to the standard investigation protocol of official health agencies. to t ... | 2020 | 32153521 |
| zika virus non-structural protein ns4a restricts eye growth in drosophila through regulation of jak/stat signaling. | to gain a comprehensive view of the changes in host gene expression underlying zika virus (zikv) pathogenesis, we performed whole-genome rna sequencing (rna-seq) of zikv-infected drosophila adult flies. rna-seq analysis revealed that zikv infection alters several and diverse biological processes, including stress, locomotion, lipid metabolism, imaginal disc morphogenesis and regulation of jak/stat signaling. to explore the interaction between zikv infection and jak/stat signaling regulation, we ... | 2020 | 32152180 |
| screening and exclusion of zika virus infection in travellers by an ns1-based elisa and qrt-pcr. | zika virus (zikv) infection during pregnancy may cause neurological abnormalities in the foetus, and therefore fast and accurate laboratory assays are critical for rapid diagnosis. elisa based on zikv ns1 protein has been developed and shown to be sensitive and highly specific; however, its negative and positive predictive values have not been tested. in this study we evaluated the ability of the ns1-based elisa to exclude zikv infection and serve as a first-line screening tool for travellers. | 2020 | 32151598 |
| single cell immune profiling of dengue virus patients reveals intact immune responses to zika virus with enrichment of innate immune signatures. | the genus flavivirus contains many mosquito-borne human pathogens of global epidemiological importance such as dengue virus, west nile virus, and zika virus, which has recently emerged at epidemic levels. infections with these viruses result in divergent clinical outcomes ranging from asymptomatic to fatal. myriad factors influence infection severity including exposure, immune status and pathogen/host genetics. furthermore, pre-existing infection may skew immune pathways or divert immune resourc ... | 2020 | 32150565 |
| c19orf66 interrupts zika virus replication by inducing lysosomal degradation of viral ns3. | the rapidly emerging human health crisis associated with the zika virus (zikv) epidemic and its link to severe complications highlights the growing need to identify the mechanisms by which zikv accesses hosts. interferon response protects host cells against viral infection, while the cellular factors that mediate this defense are the products of interferon-stimulated genes (isgs). although hundreds of isgs have been identified, only a few have been characterized for their antiviral potential, ta ... | 2020 | 32150556 |
| modelling the effective reproduction number of vector-borne diseases: the yellow fever outbreak in luanda, angola 2015-2016 as an example. | the burden of vector-borne diseases (dengue, zika virus, yellow fever, etc.) gradually increased in the past decade across the globe. mathematical modelling on infectious diseases helps to study the transmission dynamics of the pathogens. theoretically, the diseases can be controlled and eventually eradicated by maintaining the effective reproduction number, ( reff ), strictly less than 1. we established a vector-host compartmental model, and derived ( reff ) for vector-borne diseases. the analy ... | 2020 | 32149023 |
| female-to-male sexual transmssion of zika virus infection. | 2019 | 32148719 | |
| zika, dengue, chikungunya and yellow fever infections in europe? - winter is over, warm days are coming - so hedge your bets. | 2020 | 32145385 | |
| zika virus infection in chemosensory cells. | zika virus (zikv) is an emerging virus belonging to the genus flavivirus. zikv infection is a significant health concern, with increasing numbers of reports of microcephaly cases in fetuses and guillain-barré syndrome (gbs) in adults. interestingly, chemosensory disturbances are also reported as one of the manifestations of gbs. zikv infects several human tissues and cell types in vitro and in vivo. however, there is no study demonstrating zikv infection and replication in chemosensory cells, in ... | 2020 | 32144727 |
| maternal and congenital infections arising from zika, dengue and chikungunya arboviruses in salvador, brazil. | salvador was one of the brazilian cities most affected during the 2015 zika virus (zikv) outbreak. | 2020 | 32142153 |
| axonal variants of guillain-barré syndrome: an update. | axonal variants of guillain-barré syndrome (gbs) mainly include acute motor axonal neuropathy, acute motor and sensory axonal neuropathy, and pharyngeal-cervical-brachial weakness. molecular mimicry of human gangliosides by a pathogen's lipooligosaccharides is a well-established mechanism for campylobacter jejuni-associated gbs. new triggers of the axonal variants of gbs (axonal gbs), such as zika virus, hepatitis viruses, intravenous administration of ganglioside, vaccination, and surgery, are ... | 2020 | 32140865 |
| ocular manifestations in congenital zika syndrome: about a case of torpedo maculopathy. | to describe pertinent imaging studies and clinical features of a torpedo maculopathy presumably associated with congenital zika syndrome. | 2020 | 32140614 |
| strain-dependent activity of zika virus and exposure history in serological diagnostics. | zika virus (zikv) circulates as two separate lineages, with significant genetic variability between strains. strain-dependent activity has been reported for dengue virus, herpes simplex virus and influenza. strain-dependent activity of subject specimens to a virus could be an impediment to serological diagnosis and vaccine development. in order to determine whether zikv exhibits strain-dependent activity when exposed to antibodies, we measured the neutralizing properties of polyclonal serum and ... | 2020 | 32138262 |
| ire1α promotes zika virus infection via xbp1. | zika virus (zikv) is an emergent member of the flaviviridae family which causes severe congenital defects and other major sequelae, but the cellular processes that support zikv replication are incompletely understood. related flaviviruses use the endoplasmic reticulum (er) as a membranous platform for viral replication and induce er stress during infection. our data suggest that zikv activates ire1α, a component of the cellular response to er stress. ire1α is an er-resident transmembrane protein ... | 2020 | 32138181 |
| the broad spectrum antiviral ivermectin targets the host nuclear transport importin α/β1 heterodimer. | infection by rna viruses such as human immunodeficiency virus (hiv)-1, influenza, and dengue virus (denv) represent a major burden for human health worldwide. although rna viruses replicate in the infected host cell cytoplasm, the nucleus is central to key stages of the infectious cycle of hiv-1 and influenza, and an important target of denv nonstructural protein 5 (ns5) in limiting the host antiviral response. we previously identified the small molecule ivermectin as an inhibitor of hiv-1 integ ... | 2020 | 32135219 |
| strong cd4 t cell responses to zika virus antigens in a cohort of dengue virus immune mothers of congenital zika virus syndrome infants. | background: there is an urgent need to understand the complex relationship between cross-reactive anti-viral immunity, disease susceptibility, and severity in the face of differential exposure to related, circulating flaviviruses. co-exposure to dengue virus and zika virus in brazil is a case in point. a devastating aspect of the 2015-2016 south american zika outbreak was the dramatic increase in numbers of infants born with microcephaly to mothers exposed to zika virus during pregnancy. it has ... | 2020 | 32132999 |
| virus like particles (vlp) as multivalent vaccine candidate against chikungunya, japanese encephalitis, yellow fever and zika virus. | mosquito borne viral diseases are an emerging threat as evident from the recent outbreak of zika virus (zikv) as well as repeated outbreaks of chikungunya (chikv), yellow fever (yfv) and japanese encephalitis (jev) virus in different geographical regions. these four arboviruses are endemic in overlapping regions due to the co-prevalence of the transmitting mosquito vector species aedes and culex. thus, a multivalent vaccine that targets all four viruses would be of benefit to regions of the worl ... | 2020 | 32132648 |
| very-long-chain fatty acid metabolic capacity of 17-beta-hydroxysteroid dehydrogenase type 12 (hsd17b12) promotes replication of hepatitis c virus and related flaviviruses. | flaviviridae infections represent a major global health burden. by deciphering mechanistic aspects of hepatitis c virus (hcv)-host interactions, one could discover common strategy for inhibiting the replication of related flaviviruses. by elucidating the hcv interactome, we identified the 17-beta-hydroxysteroid dehydrogenase type 12 (hsd17b12) as a human hub of the very-long-chain fatty acid (vlcfa) synthesis pathway and core interactor. here we show that hsd17b12 knockdown (kd) impairs hcv repl ... | 2020 | 32132633 |
| a novel neutralizing antibody targeting a unique cross-reactive epitope on the hi loop of domain ii of the envelope protein protects mice against duck tembusu virus. | the flavivirus e protein induces protective immunity, and its abs cause serious problems for serodiagnosis because of the difficulty in differentiating cross-reactive abs. moreover, cross-reactive abs may increase disease severity after secondary flavivirus infections via ab-dependent enhancement. cross-reactive epitopes are therefore critical for understanding serodiagnosis and improving the general knowledge of flavivirus infections. a minimal epitope, 227gssagtwqn235, was identified by a neut ... | 2020 | 32132180 |
| zika virus infection in the genital tract of non-pregnant females: a systematic review. | this review provides a general overview on the positivity and persistence of zika virus (zikv) in female genital tract (fgt) of non-pregnant women and animals, as well as in cell cultures, and its influence on fgt health. we performed a systematic review based on the prisma statement to identify studies focused on "zika virus" and "non-pregnant female" in pubmed, embase, scopus scholar and web of knowledge databases of full-text papers and abstracts published in english, with no restrictions reg ... | 2020 | 32130356 |
| the journey of zika to the developing brain. | zika virus is a mosquito-borne flavivirus originally isolated from humans in 1952. following its re-emergence in brazil in 2015, an increase in the number of babies born with microcephaly to infected mothers was observed. microcephaly is a neurodevelopmental disorder, characterised phenotypically by a smaller than average head size, and is usually developed in utero. the 2015 outbreak in the americas led to the world health organisation declaring zika a public health emergency of international c ... | 2020 | 32128708 |
| the gut anti-complement activity of aedes aegypti: investigating new ways to control the major human arboviruses vector in the americas. | aedes aegypti is the main urban vector of dengue virus, chikungunya virus and zika virus due to its great dispersal capacity and virus susceptibility. a. aegypti feed on plant-derived sugars but females need a blood meal for egg maturation. haematophagous arthropods need to overcome host haemostasis and local immune reactions in order to take a blood meal. in this context, molecules present in the saliva and/or intestinal contents of these arthropods must contain inhibitors of the complement sys ... | 2020 | 32126277 |