Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| occurrence of group a rotavirus mixed p genotypes infections in children living in goiânia-goiás, brazil. | group a rotaviruses (rva) are the main causing agents of acute gastroenteritis worldwide, having a great impact on childhood mortality in developing countries. the objective of this study was to identify rva-positive fecal samples with mixed p genotypes by hemi-nested reverse transcriptase-polymerase chain reaction (rt-pcr), followed by sequencing confirmation. our results showed that, from the 81 rva-positive samples, 25 were positive for more than one p genotype by hemi-nested rt-pcr. of these ... | 2008 | 18521634 |
| [epidemiology and burden of rotavirus diarrhea in day care centers in lyon, france]. | rotavirus is the main cause of severe, dehydrating diarrhoea in infants and young children. in industrialized countries, pediatric rotavirus gastroenteritis (prge) is responsible for high morbidity, particularly among children under 3 years of age attending day care centers (dccs). the objectives of this study were to estimate the incidence, management and cost of prge in dccs. we also described the nature of group a rotavirus genotypes. this study also compared the performance of different diag ... | 2008 | 18456480 |
| hospitalization due to norovirus and genotypes of rotavirus in pediatric patients, state of espírito santo. | viruses are the leading cause for hospitalization due to gastroenteritis worldwide. group a rotaviruses (rv) are the most prevalent and are assorted in glycoproteins (g) and protease sensitive (p) dual genotypes based on polymorphic genes that encode the external vp7 and vp4 capsid proteins, respectively. noroviruses (nov) have increasingly answered by sporadic gastroenteritis. this study aimed to determine the prevalence of nov and rv in 68 hospitalized children, between july 2004 and november ... | 2008 | 18425274 |
| porcine rotavirus bearing an aberrant gene stemming from an intergenic recombination of the nsp2 and nsp5 genes is defective and interfering. | serial undiluted passage of a porcine rotavirus in ma104 cells yielded three distinct virus populations, each of which bore different rearranged genes. sequencing revealed that each of two populations bore a distinct intragenic recombinant nsp3 gene consisting of a partial duplication in a head-to-tail orientation without altering the nsp3 open reading frame and the third population carried both an intragenic recombinant nsp3 gene and an intergenic recombinant gene (1,647 nucleotides in length) ... | 2008 | 18417592 |
| design of a multiplex nested pcr for genotyping of the nsp4 from group a rotavirus. | a novel pcr method was developed to discriminate amongst genotypes a-c of the rotavirus non-structural protein 4 (nsp4). genotype-specific primers were designed that correctly identified the nsp4 genotype when evaluated as a multiplex pcr with cell culture adapted rotavirus strains. rotavirus strains b223 sgig6p6[1], ncdv sgig6p6[1] and sa11 sgig3p5b[2] were used as control for nsp4 genotype a; a34 sgig5p14[23], gottfried sgiig4p2b[6] and wa sgiig1p1a[8] for nsp4 genotype b; rrv sgig3p5b[3] for ... | 2008 | 18353449 |
| detection and characterization of human caliciviruses associated with sporadic acute diarrhea in adults in djibouti (horn of africa). | recent advances in molecular diagnostics have allowed us to recognize human caliciviruses (hucvs) as important agents of acute diarrhea in industrialized countries. their prevalence and genetic diversity in developing countries remains unknown. we report on the characterization of hucvs among adults presenting acute diarrheas in djibouti; 108 stool samples collected were screened by eia, rtpcr, or cell cultures for the group a rotaviruses, adenoviruses, astroviruses, and hucvs, which were furthe ... | 2008 | 18337354 |
| full genomic analysis of human rotavirus strain tb-chen isolated in china. | a g2p[4]/nsp4[a] rotavirus strain tb-chen was isolated from a 2-year-old patient hospitalized with acute gastroenteritis in kunming, china. the strain tb-chen was demonstrated having group a-specific antigenicity, a "short" (subgroup ii) electropherotype. to investigate its overall genomic relatedness and to determine which group it belonged, the complete genome of strain tb-chen was determined. genomic comparison based on amino acid sequence identity and phylogenetic analysis revealed that all ... | 2008 | 18329063 |
| [genetic variation in vp7 gene of rotavirus serotype g3 predominated in changchun, china]. | group a rotavirus (rv) is the most important etiologic agent of severe gastroenteritis among children and the development of an effective vaccine becomes the top public health priority. since survey of rv serotypes circulating in local community is important for introduction or development of rv vaccine, rv serotype g3 had proved as the predominant strain in changchun from 2001 to 2005. stool specimens collected from children with acute diarrhea were tested for group a rotavirus by enzyme-linked ... | 2008 | 18320818 |
| is rotavirus a hepatotropic virus? | 2008 | 18264761 | |
| emergence of human rotavirus group a genotype g9 strains, wuhan, china. | of 322 stool specimens collected from children with diarrhea from october 2005 through september 2006 in wuhan, china, group a rotavirus was identified in 101 (31.4%). the most prevalent group a rotavirus genotype was g3p[8] (62.6%), followed by g1p[8](17.6%), g1+g3p[8](8.8%), g3p[4](6.6%), g1p[4](2.2%), and g9p[8](2.2%). the g9 strains were first detected in wuhan. | 2007 | 18258015 |
| development of a rapid and sensitive latex agglutination-based method for detection of group a rotavirus. | considering the background of morbidity and mortality caused by human rotavirus, detection methods that use rotavirus group antigen (vp6) in either enzyme immunoassay (eia) or latex agglutination test (lat) has been employed routinely in clinical diagnostic and epidemiological studies. in order to develop a rapid and sensitive rotavirus group a lat, part of segment 6 corresponding to conserved n-terminal portion of the vp6 (1-245 aa) was cloned in escherichia coli expression pgex vector (glutath ... | 2008 | 18241934 |
| multiple combinations of p[13]-like genotype with g3, g4, and g5 in porcine rotaviruses. | epidemiological surveillance of porcine rotavirus (porv) strains was carried out in chiang mai province, thailand, from 2002 to 2003, and eight rotavirus isolates could not be completely typed by pcr. of these, six were g3 and one was g4 and displayed a p-nontypeable genotype, while another isolate was both g and p nontypeable. analysis of a partial vp4 gene of all eight p-nontypeable strains revealed a high degree of amino acid sequence identities (94.7% to 100%), suggesting that they belonged ... | 2008 | 18234859 |
| molecular characterization of group a human rotaviruses in bangkok and buriram, thailand during 2004-2006 reveals the predominance of g1p[8], g9p[8] and a rare g3p[19] strain. | the aim of this study has been to determine the incidence of diverse human rotavirus strains circulating in thailand between october 2004 and april 2006 by means of molecular characterization. pediatric patients aged between 2 months and 5 years diagnosed with acute diarrhea (n=307) in bangkok and buriram, thailand were tested for human rotavirus a (rv-a) by rt-pcr. a total of 130 specimens (42.3%) were found rv-a positive and 126 were characterized by direct sequencing of the capsid glycoprotei ... | 2008 | 18228123 |
| molecular characterization of a subgroup specificity associated with the rotavirus inner capsid protein vp2. | group a rotaviruses are classified into serotypes, based on the reactivity pattern of neutralizing antibodies to vp4 and vp7, as well as into subgroups (sgs), based on non-neutralizing antibodies directed against vp6. the inner capsid protein (vp2) has also been described as a sg antigen; however, little is known regarding the molecular determinants of vp2 sg specificity. in this study, we characterize vp2 sgs by correlating genetic markers with the immunoreactivity of the sg-specific monoclonal ... | 2008 | 18216104 |
| full genome-based classification of rotaviruses reveals a common origin between human wa-like and porcine rotavirus strains and human ds-1-like and bovine rotavirus strains. | group a rotavirus classification is currently based on the molecular properties of the two outer layer proteins, vp7 and vp4, and the middle layer protein, vp6. as reassortment of all the 11 rotavirus gene segments plays a key role in generating rotavirus diversity in nature, a classification system that is based on all the rotavirus gene segments is desirable for determining which genes influence rotavirus host range restriction, replication, and virulence, as well as for studying rotavirus epi ... | 2008 | 18216098 |
| rearrangements of rotavirus genomic segment 11 are generated during acute infection of immunocompetent children and do not occur at random. | group a rotaviruses are the main cause of viral gastroenteritis in infants. the viral genome consists of 11 double-stranded rna (dsrna) segments. dysfunction of the viral rna polymerase can lead to gene rearrangements, which most often consist of partial sequence duplication of a dsrna segment. gene rearrangements have been detected in vivo during chronic infection in immunodeficient children or in vitro during passages at a high multiplicity of infection in cell culture, suggesting that these r ... | 2008 | 18216096 |
| expression and characterization of vp2 protein of human rotavirus a in a mammalian lung cell line. | a system for the expression and characterization of vp2 protein of human rotavirus a, strain g3 was established in the mammalian lung cell line (a549). expression of the recombinant vp2 (rvp2) protein was detected 48-72 hrs after transfection by western blotting. the rvp2 protein expressed in a549 cells formed intracellular core-like particles (clps) 30 nm in diameter detected by electron microscopy (em). these results showed that the a549 cells are suitable as efficient eukaryotic host for prod ... | 2007 | 18197733 |
| development of a method for concentrating and detecting rotavirus in oysters. | identification of enteric viruses in outbreak-implicated bivalve shellfish is difficult because of low levels of contamination and natural inhibitors present in shellfish tissue. in this study, the acid adsorption-alkaline elution method developed in our laboratory was proposed for the detection of rotavirus from oyster samples. the acid adsorption-alkaline elution process included the following steps: acid adsorption at ph 4.8, elution with 2.9% tryptose phosphate broth containing 6% glycine, p ... | 2008 | 18177969 |
| prevalence of enteropathogenic viruses and molecular characterization of group a rotavirus among children with diarrhea in dar es salaam tanzania. | different groups of viruses have been shown to be responsible for acute diarrhea among children during their first few years of life. epidemiological knowledge of viral agents is critical for the development of effective preventive measures, including vaccines. | 2007 | 18162127 |
| [comparison of clinical characteristics of patients with rotavirus gastroenteritis relative to the infecting rotavirus g-p genotype.]. | group a rotavirus is a major cause of severe diarrhea in children throughout the world. for the proper management of rotavirus infections, it will be helpful to know their clinical characteristics according to the g and p genotypes of the infecting virus. | 2006 | 18156706 |
| development of a microtiter plate hybridization-based pcr-enzyme-linked immunosorbent assay for identification of clinically relevant human group a rotavirus g and p genotypes. | a microtiter plate hybridization-based pcr-enzyme-linked immunosorbent assay (pcr-elisa) has been used for the detection and identification of a variety of microorganisms. here, we report the development of a pcr-elisa for the identification of clinically relevant human rotavirus vp7 (g1 to g6, g8 to g10, and g12) and vp4 (p[4], p[6], p[8], p[9], and p[14]) genotypes. the g and p types of reference human and animal rotavirus strains for which specific probes were available were correctly identif ... | 2008 | 18057127 |
| detection of unusual rotavirus genotypes g8p[8] and g12p[6] in south korea. | five hundred four fecal specimens, collected between 2004 and 2006 from young children with acute diarrhea, were screened for rotavirus by elisa with vp6-specific antibody. of these samples, 394 (78.2%) were confirmed as group a rotavirus and they underwent g- and p typing using a combination of elisa, rt-pcr, and sequence analysis methods. the dominant circulating g serotype was g1 (35.6%) followed by g3 (26.4%), g4 (14.7%), and g2 (11.9%). there was a low prevalence of g9 (1.0%) and of unusual ... | 2008 | 18041003 |
| [characteristic of the etiologic structure of acute enteric infections in adults based on the data from infectious diseases hospital]. | study of the etiologic spectrum of sporadic cases of causative agents of acute enteric infections (aei) in 753 patients aged 15-85 years old using polymerase chain reaction (pcr) was performed in the infectious diseases hospital during 12 months. it was shown that detection of the causative agents of aei by pcr was 2.2 times more effective than routine microbiologic diagnostics supplemented with test of feces on rotavirus by elisa method and allowed to detect etiologic agent in 54.3% of the pati ... | 2007 | 18038539 |
| group a rotavirus-associated diarrhea in children seeking treatment in indonesia. | globally, group a rotavirus causes significant morbidity and mortality among children. limited data exist on the epidemiology of rotavirus disease among indonesian children. | 2007 | 17977785 |
| characterization of clinical and immune response in a rotavirus diarrhea model in suckling lewis rats. | group a rotaviruses (rvs) are the leading pathogens causing diarrhea in children and animals. the present study was designed to establish an experimental model of rv infection and immune response in suckling rats. wistar (w) and lewis (l) suckling rats were inoculated orally with two different doses of a simian rv sa-11 strain. rv infection was evaluated by growth rate and clinical indexes. virus-shedding and serum anti-rv antibodies were measured by enzyme-linked immunosorbent assay (elisa). mu ... | 2007 | 17957154 |
| [eurorotanet--european rotavirus strain surveillance network established with hungarian participation]. | group a rotaviruses are the most common cause of severe gastroenteritis worldwide. the incidence and distribution of group a rotavirus sero/genotypes varies between geographical areas during a rotavirus season, and from one season to the next. in addition, cocirculation of genetically diverse multitypic rotaviruses and of intratypic variants in any one place and time is common. assuming widespread use of rotavirus vaccine in the near future, comprehensive surveillance of natural rotavirus infect ... | 2007 | 17947197 |
| [rotavirus a among hospitalized infants, up to three years of age, with acute gastroenteritis in campo grande, state of mato grosso do sul]. | polyacrylamide gel electrophoresis and combined immunoenzyme assay for rotavirus and adenovirus were used to analyze 380 fecal samples from children up to three years of age who were hospitalized with acute diarrhea in campo grande, state of mato grosso do sul, between may 2000 and january 2004. among all the samples, 88 (23.2%) were positive for rotavirus a. out of these, 81 (92%) had a defined electrophoretic pattern: 77 (87.5%) with a long pattern and four (4.5%) with a short pattern. genotyp ... | 2007 | 17876462 |
| changing pattern of rotavirus g genotype distribution in chiang mai, thailand from 2002 to 2004: decline of g9 and reemergence of g1 and g2. | group a rotaviruses are the most common cause of acute viral diarrhea in humans and animals throughout the world. previous surveillance studies of group a rotaviruses in thailand indicated that the dominant types of rotaviruses were changing from time to time. during 2000 and 2001, the g9 rotavirus emerged as the most prevalent genotype, with an exceptionally high frequency (91.6%) in chiang mai, thailand. in the year 2002-2004, group a rotavirus was detected in 98 out of 263 (37.3%) fecal speci ... | 2007 | 17854032 |
| an atpase activity associated with the rotavirus phosphoprotein nsp5. | interactions between nsp5 and nsp2 drive the formation of viroplasms, sites of genome replication and packaging in rotavirus-infected cells. the serine-threonine-rich nsp5 transitions between hypo- and hyper-phosphorylated isomers during the replication cycle. in this study, we determined that purified recombinant nsp5 has a mg2+-dependent atp-specific triphosphatase activity that generates free adp and pi (vmax of 19.33 fmol of product/min/pmol of enzyme). the atpase activity was correlated wit ... | 2007 | 17825341 |
| impact of porcine group a rotavirus co-infection on porcine epidemic diarrhea virus pathogenicity in piglets. | porcine epidemic diarrhea virus (pedv) and porcine group a rotavirus (pgar) are the main causative agents of acute diarrhea in piglets. in south korea, pgar is prevalent in piglets naturally infected with pedv. piglets naturally co-infected with pedv and pgar appeared to have severe and prolonged diarrhea that was distinct from that commonly observed. the aim of this study was to determine the impact of pgar co-infection on pedv pathogenicity in piglets. thirty-six colostrum-deprived, one-day ol ... | 2008 | 17727905 |
| diversity of group a rotavirus strains circulating in paraguay from 2002 to 2005: detection of an atypical g1 in south america. | group a rotaviruses are the main cause of severe gastroenteritis in children worldwide. | 2007 | 17720620 |
| rotavirus and adenovirus in rondônia. | acute gastroenteritis is one of the most common diseases in humans worldwide. viral gastroenteritis is a global problem in infants and young children. in this study the incidence of diarrhea was assessed in 877 hospitalized children under five years old, over a period of 24 months and distributed in 470 cases of diarrhea and 407 age-matched group with other pathologies, as control group. two antigen detection techniques based on enzyme immunoassay (eia) and latex particles were used for detectio ... | 2007 | 17710298 |
| the formation of viroplasm-like structures by the rotavirus nsp5 protein is calcium regulated and directed by a c-terminal helical domain. | the rotavirus nsp5 protein directs the formation of viroplasm-like structures (vls) and is required for viroplasm formation within infected cells. in this report, we have defined signals within the c-terminal 21 amino acids of nsp5 that are required for vls formation and that direct the insolubility and hyperphosphorylation of nsp5. deleting c-terminal residues of nsp5 dramatically increased the solubility of n-terminally tagged nsp5 and prevented nsp5 hyperphosphorylation. computer modeling and ... | 2007 | 17699573 |
| detection and identification of echovirus 7 from a child with gastro-enteritis. | gastro-enteritis continues to be a significant cause of mortality in infants and young children in developing countries. some previous studies have associated echoviruses with gastroenteritis. | 2006 | 17685211 |
| phylogenetic analysis of porcine rotavirus in argentina: increasing diversity of g4 strains and evidence of interspecies transmission. | group a rotaviruses are one of the most frequently detected viral agents associated with neonatal diarrhea in piglets. in order to characterize rotavirus (rv) strains circulating in argentinean swine, four porcine production farms located in buenos aires were studied. rv strains genotyped as p[6]g4, p[6]g8 and p[1]g6 were found in piglets under 30 days of age, without diarrhea. phylogenetic and sequence analysis of the vp7 gene from g4 strains available in databases, reveals five porcine new lin ... | 2008 | 17659850 |
| characterization of the nsp6 protein product of rotavirus gene 11. | the 12kda non-structural protein 6 (nsp6) is the least studied of the rotavirus proteins. in an attempt to further characterize this protein mono-specific antisera was generated using purified protein expressed in e. coli. pulse/chase radio-labeling of virus infected cells was used to show that it is expressed at a steady but low rate throughout the virus replication cycle. in contrast to the other rotavirus non-structural proteins, nsp6 was found to have a high rate of turnover, being completel ... | 2007 | 17658646 |
| electropherotypes of rotaviruses in children less than 5 years old in abidjan, cote d'ivoire in 1997. | rotaviruses are associated with gastroenteritis in human infants and the young of many species. in this study, we analysed the circulating strains of human rotavirus isolated from young children in abidjan by electrophoresis of the viral rna genome in agarose gels. rotavirus strains were identified in 33 children less than two years of age in the yopougon district of abidjan, cote d'lvoire. viral rna was extracted from the stools by phenol-chloroform treatment at 56 degrees c, followed by centri ... | 2001 | 17650045 |
| rotavirus infection among infants and young children in chennai, south india. | group a rotavirus has been recognized as the major etiologic agent of childhood gastroenteritis in infants and young children worldwide. rapid progress towards the development of an efficacious rotavirus vaccine has warranted extensive epidemiological studies on rotavirus serotypes that cause severe disease in developing and developed countries and to monitor the emergence of newer and unusual strains in different geographical settings that could represent variants not covered by existing vaccin ... | 2004 | 17642741 |
| burden of disease & molecular epidemiology of group a rotavirus infections in india. | rotavirus is the major cause of severe dehydrating diarrhoea in young children worldwide. considerable research has been carried out on rotavirus disease in india. this review collated data from 46 epidemiological studies to determine rotavirus positivity rates and genotypes of infecting rotavirus strains from various settings in india. studies on diarrhoea presenting to hospitals, neonatal rotavirus infections, symptomatic and asymptomatic infections in the community and nosocomial enteric infe ... | 2007 | 17642497 |
| molecular characterization of group a rotavirus isolates obtained from hospitalized children in salvador, bahia, brazil. | rotavirus is a major cause of infectious diarrhea in infants and young children. the objective of this study was to characterize the genotypes of human rotavirus found in children hospitalized with acute diarrhea in the pediatric hospital prof. hosannah de oliveira of the ufba in salvador, bahia, brazil, during the years of 1999, 2000 and 2002. fecal samples were analyzed (n=358) by methods eiara and sds-page for detection of rotavirus. positive samples of one or two of these methods (n=168) wer ... | 2007 | 17625724 |
| effects of different animal waste treatment technologies on detection and viability of porcine enteric viruses. | enteric pathogens in animal waste that is not properly processed can contaminate the environment and food. the persistence of pathogens in animal waste depends upon the waste treatment technology, but little is known about persistence of porcine viruses. our objectives were to characterize the porcine enteric viruses (porcine noroviruses [ponovs], porcine sapoviruses [posavs], rotavirus a [rv-a], rv-b, and rv-c) in fresh feces or manure and to evaluate the effects of different candidate environm ... | 2007 | 17601821 |
| [g[p] type profiles of group a human rotavirus and their distribution in nizhni novgorod and dzerzhinsk in 1997-2005]. | rt-pcr was used to determine g (vp7) and p (vp4) genotypes of group a rotavirus found in 2454 children with diarrhea in 1997-2005. eight g[p] combinations, including g1-4 serotypes and p[4, 6, 8, 9] genotypes, were identified. the p[8] genotype was presented by the subtypes p[8]-1 and p[8]-2. in 1997-2005, the spectrum and distribution of g[p] types were as follows: g1p[8]-1 (10%), g1p[8]-2 (67%), g1p[6] (3%), g2p[4] (9%), g3p[8]-2 (5%), g3p[6] (1%), g3p[9] (1%), g4p[8]-2 (3%), g9 (0%), g?p[?] ( ... | 2007 | 17601046 |
| rotavirus infection in the paraguayan population from 2004 to 2005: high incidence of rotavirus strains with short electropherotype in children and adults. | rotavirus is considered the main viral cause of acute gastroenteritis in children in both developed and developing countries. the aim of the present study was to continue the surveillance of rotavirus in the paraguayan population in anticipation of a rotavirus vaccination in children. | 2007 | 17599029 |
| serotype 1 and 2 bovine noroviruses are endemic in cattle in the united kingdom and germany. | the genomically and antigenically distinct bovine noroviruses bo/jena/1980/de and bo/newbury2/1976/uk have been associated with calf diarrhea. in the present seroprevalence study, both were found to be endemic in cattle from germany and the united kingdom, a finding in contrast to previous virus prevalence studies. they were less common than group a rotaviruses, particularly in calves, suggesting a different epidemiology. | 2007 | 17596356 |
| human group c rotaviruses identified in nigerian children. | a survey investigating the prevalence of rotavirus infection in infants and young children with acute diarrhoea was undertaken in jos state, nigeria during the first half of 1998. in total, 182 faecal specimens were collected from children aged between 1 and 60 months with acute infantile gastroenteritis. examination with a commercial elisa assay for the presence of group a rotavirus antigen revealed 16 specimens positive for this antigen. however, examination of the double stranded rna of the v ... | 1998 | 17581014 |
| molecular characterization of bovine group a rotavirus g3p[3] strains. | during a surveillance study, four of 130 group a rotavirus strains, detected from diarrheic calves in eastern india, exhibited g3p[3] specificities. molecular characterization of vp7 and vp8(*) genes of one such strain [named as rubv3 (ru: ruminant and bv: bovine)] revealed genetic relatedness to a g3p[3] simian strain, rrv, and rrv-related caprine strain grv. strain rubv3 had vp6, nsp4 and nsp5 genes of bovine origin. therefore, the present study provides evidence for multiple reassortment even ... | 2007 | 17577612 |
| development and validation of dna microarray for genotyping group a rotavirus vp4 (p[4], p[6], p[8], p[9], and p[14]) and vp7 (g1 to g6, g8 to g10, and g12) genes. | previously, we reported the development of a microarray-based method for the identification of five clinically relevant g genotypes (g1 to g4 and g9) (v. chizhikov et al., j. clin. microbiol. 40:2398-2407, 2002). the expanded version of the rotavirus microarray assay presented herein is capable of identifying (i) five clinically relevant human rotavirus vp4 genotypes (p[4], p[6], p[8], p[9], and p[14]) and (ii) five additional human rotavirus vp7 genotypes (g5, g6, g8, g10, and g12) on one chip. ... | 2007 | 17567783 |
| [two cases of group a rotavirus outbreak among adults]. | two cases of group infections with the group a rotavirus (arv) occurred among adult residents at welfare facilities. case 1 occurred at a care facility for the elderly between march and april of 2000, where 14 patients aged 68 to 93 (average: 85) of 93 residents developed cardinal symptoms of diarrhea, vomiting and fever. arv was found in 7 stool samples and their g-serotype was g2. case 2 occurred at a facility for the disabled in april 2006, where 15 patients aged 28 to 64 (average: 48) of 51 ... | 2007 | 17564113 |
| group a rotavirus genotypes in germany during 2005/2006. | during the 2005/2006 winter season a total of 802 group a positive rotavirus specimens of patients from different regions throughout germany were genotyped. amplicons from a one-tube rt-pcr were typed by analysis of their (type-specific) size using type-specific primers, fluorescent consensus primers and a capillary sequencer for detection. while g1p[8] was predominant (45.8%), g9p[8] has emerged as the second most frequent genotype combination (37.7%). the distribution of genotypes was heteroge ... | 2007 | 17557132 |
| evidence for bovine origin of vp4 and vp7 genes of human group a rotavirus g6p[14] and g10p[14] strains. | 2007 | 17537935 | |
| group a rotavirus strains circulating in the eastern center of tunisia during a ten-year period (1995-2004). | an epidemiological survey investigating rotavirus infections in children was undertaken in the eastern center of tunisia between january 1995 and december 2004. a total of 982 faecal specimens collected from children less than 5 years in age were screened by enzyme-linked immunosorbent assay (elisa) or latex agglutination assay for the presence of group a rotavirus antigen. rotavirus-positive samples were used for g and p typing by multiplex semi-nested reverse transcription-pcr. rotaviruses wer ... | 2007 | 17516524 |
| detection of the rotavirus a genome from the cerebrospinal fluid of a gastroenteritis patient: a case report. | 2007 | 17515656 | |
| molecular analysis of the nsp4 and vp6 genes of rotavirus strains recovered from hospitalized children in rio de janeiro, brazil. | group a rotaviruses are the main cause of acute gastroenteritis in children throughout the world. the two outer capsid proteins, vp4 and vp7, define the p and g genotypes, respectively. rotaviruses with p[8]g1, p[4]g2, p[8]g3 and p[8]g4 genotypes are predominant in infecting humans and the g9 genotype is emerging in most continents as the fifth most common g type worldwide. the inner capsid protein vp6 is responsible for subgroup (sg) specificities, allowing classification of rotaviruses into sg ... | 2007 | 17510274 |
| evaluation of immunochromatography tests for detection of rotavirus and norovirus among vietnamese children with acute gastroenteritis and the emergence of a novel norovirus gii.4 variant. | a prospective study was conducted to evaluate two immunochromatography (icg) tests for detection of group a rotavirus and norovirus gii, the commercial dipstick 'eiken' rota kit (sa scientific, usa) and the nv ic-1 stick (immuno-probe, japan). polymerase chain reaction (pcr) with specific primer pairs (beg9 and vp7-1', for group a rotavirus; cog2f and g2skr, for norovirus gii) was used as the reference method. the results of icg tests were compared with those of reference method. the sensitivity ... | 2007 | 17496324 |
| molecular characterization of rare g3p[9] rotavirus strains isolated from children hospitalized with acute gastroenteritis. | in 2004, an epidemiological survey of human rotavirus infection in chiang mai, thailand detected two uncommon human rotavirus strains (cmh120/04 and cmh134/04) bearing au-1-like g3p[9] genotypes in 1 year old children hospitalized with acute gastroenteritis. the cmh120/04 and cmh134/04 rotavirus strains were characterized by molecular analyses of their vp6, vp7, vp8*, and nsp4 gene segments as well as the determination of rna patterns by polyacrylamide gel electrophoresis (page). analysis of the ... | 2007 | 17457907 |
| detection by pcr of eight groups of enteric pathogens in 4,627 faecal samples: re-examination of the english case-control infectious intestinal disease study (1993-1996). | the english case-control infectious intestinal disease study (1993-1996) failed to detect an enteric pathogen or toxin in 49% of cases of gastroenteritis. in the present study, polymerase chain reaction (pcr) assays were applied to dna and cdna generated from 4,627 faecal samples from cases and controls archived during the original study for the detection of norovirus, rotavirus, sapovirus, campylobacter spp., salmonella spp., enteroaggregative escherichia coli, cryptosporidium spp., and giardia ... | 2007 | 17447091 |
| prevalence of group c rotaviruses in weaning and post-weaning pigs with enteritis. | diarrheic fecal specimens collected from porcine herds were screened for the presence of group c rotaviruses using a reverse transcription-polymerase chain reaction (rt-pcr) assay. a total of 188 samples were tested and 54 were positive. when compiled these data with diagnostic results on group a rotaviruses and enteric caliciviruses we found that all but 5 group c rotavirus positive samples contained at least one additional virus. a subset of samples were subjected to nucleotide sequencing. the ... | 2007 | 17416471 |
| diversity of viruses associated with acute gastroenteritis in children hospitalized with diarrhea in ho chi minh city, vietnam. | a molecular epidemiological study on common diarrheal viruses was conducted in ho chi minh city, vietnam between october 2002 and september 2003. fecal samples were collected from 1,010 hospitalized children with acute gastroenteritis. those samples were screened for groups a, b, and c rotavirus, adenovirus, genogroups i and ii norovirus (nov), sapovirus (sav), and human astrovirus (hastv) by rt-multiplex pcr, and the positive specimens were characterized further by elisa, nested pcr, or sequenc ... | 2007 | 17385670 |
| prevalence of g2p[4] and g12p[6] rotavirus, bangladesh. | approximately 20,000 stool specimens from patients with diarrhea visiting 1 urban and 1 rural hospital in bangladesh during january 2001-may 2006 were tested for group a rotavirus antigen, and 4,712 (24.0%) were positive. g and p genotyping was performed on a subset of 10% of the positive samples (n = 471). during the 2001-2005 rotavirus seasons, g1p[8] (36.4%) and g9p[8] (27.7%) were the dominant strains, but g2[4] and g12p[6] were present in 15.4% and 3.1% of the rotavirus-positive patients, r ... | 2007 | 17370511 |
| molecular characterization of a novel bovine group a rotavirus. | in this study, by partial sequence analysis of the genome segments encoding vp5* and vp7, we characterized a novel bovine group a rotavirus, namely, tak2, that was detected from adult cattle diarrhea in tochigi prefecture, japan. the nucleotide (nt) and deduced amino acid (aa) sequences of the genome segments encoding vp5* and half of the amino terminal portion of vp7 of tak2 revealed a low identity with those of group a rotaviruses carrying previously published p and g type specificities (vp5*: ... | 2007 | 17368763 |
| virus diversity of acute diarrhea in tropical highlands. | infectious acute diarrhea (iad) is an important health problem affecting a large number of latin-american children. several reports show that bacteria, parasites and virus are involved in the burden of this disease. most reports reveal rotavirus a as the responsible etiological agent, at the same time, there seems to be some correlation between iad and seasonal weather changes. to learn about the type of microbial agents associated with iad in children during mildly changing yearly climatic cond ... | 2006 | 17357570 |
| detection and genetic characterization of group a rotavirus strains circulating among children with acute gastroenteritis in japan. | a total of 603 fecal specimens collected from july 2005 to june 2006 from children with acute gastroenteritis, encompassing five different localities in japan, were screened for group a rotavirus by reverse transcription-pcr. it was found that 117 fecal specimens (19.4%) were positive for group a rotavirus. rotavirus infection was detected continuously from november to june, with the highest prevalence in april. the g (vp7 genotypes) and p (vp4 genotypes) types were further investigated. the pre ... | 2007 | 17301134 |
| frequency of group a rotavirus in diarrhoeic calves in brazilian cattle herds, 1998-2002. | the frequency of group a bovine rotavirus (gpa brv) in calves from 1998 to 2002 was determined by the polyacrylamide gel electrophoresis technique in 2177 faecal samples, of which 1898 samples were diarrhoeic and 279 were of normal consistency (control group) that were collected from asymptomatic calves for comparative purposes. the animals were from beef and dairy cattle herds (n = 321) from 158 counties in seven states (paraná, são paulo, minas gerais, mato grosso do sul, mato grosso, goiás an ... | 2006 | 17265766 |
| anticipating rotavirus vaccines in brazil: detection and molecular characterization of emerging rotavirus serotypes g8 and g9 among children with diarrhoea in recife, brazil. | in 2006, brazil will initiate universal immunization of its 4-million infants with a live attenuated serotype g1p[8] human rotavirus vaccine. in anticipation of the national immunization program, this study was undertaken to characterize rotavirus strains circulating among children in recife, one of the largest cities in the northeast region of brazil. group a rotaviruses were detected in 102 (35%) of 290 faecal specimens collected from children under 5 years of age who presented with acute diar ... | 2007 | 17245712 |
| coupling of rotavirus genome replication and capsid assembly. | the reoviridae family represents a diverse collection of viruses with segmented double-stranded (ds)rna genomes, including some that are significant causes of disease in humans, livestock, and plants. the genome segments of these viruses are never detected free in the infected cell but are transcribed and replicated within viral cores by rna-dependent rna polymerase (rdrp). insight into the replication mechanism has been provided from studies on rotavirus, a member of the reoviridae whose rdrp c ... | 2007 | 17222694 |
| molecular epidemiologic analysis of group a rotaviruses in adults and children with diarrhea in wuhan city, china, 2000-2006. | to compare epidemiologic features and genetic characteristics of group a rotaviruses causing diarrhea in children and adults, a survey was conducted in wuhan, china, during the period of dec. 2000-may 2006. a total of 3839 stool specimens from diarrheal patients from eight hospitals were analyzed. winter seasonality was observed for rotavirus diarrhea in both adults and children, showing overall rotavirus-positive rates of 9.0 and 23.9%, respectively. throughout the study period, g3 was the most ... | 2007 | 17195953 |
| an outbreak of adenovirus serotype 41 infection in infants and children with acute gastroenteritis in maizuru city, japan. | a total of 337 fecal specimens were collected from infants and children with acute gastroenteritis in maizuru city, japan from july 2004 to june 2005 and tested for the presence of rotavirus, norovirus, sapovirus, astrovirus, and adenovirus by rt-multiplex pcr. among diarrheal viruses detected, norovirus was the most prevalent (13.6%, 46 of 337), followed by adenovirus (8%, 27 of 337), group a rotavirus (5%, 17 of 337), astrovirus (1.8%, 6 of 337), and sapovirus (1.8%, 6 of 337), respectively. a ... | 2007 | 17157081 |
| accumulation of rotavirus vp6 protein in chloroplasts of transplastomic tobacco is limited by protein stability. | rotavirus vp6 is a highly immunogenic major capsid protein that may be useful as a subunit vaccine. the expression of a bovine group a rotavirus vp6 cdna was examined in tobacco chloroplasts following particle bombardment. constructs containing the vp6 cdna under the control of plastid rrn or psba promoters, or the escherichia coli trc promoter, were inserted, together with the aada selectable marker gene, between the rbcl and accd genes of the tobacco plastid genome. the 40-kda vp6 protein accu ... | 2004 | 17147617 |
| genetic characterization of group a rotavirus strains circulating among children with acute gastroenteritis in japan in 2004-2005. | a total of 752 fecal specimens collected from july 2004 to june 2005 from children with acute gastroenteritis in four localities in japan (maizuru, tokyo, sapporo, and osaka) were screened for group a rotavirus by rt-pcr. it was found that 82 (10.9%) specimens were positive for group a rotavirus. the g-(vp7 genotypes) and p-(vp4 genotypes) types were further investigated. the p-types of 18 rotavirus strains, which could not be typed by rt-pcr, were determined by sequencing analysis. of these, 94 ... | 2007 | 17110174 |
| molecular characterisation of the rotavirus strains prevalent in maua, meru north, kenya. | rotavirus is the most common cause of severe infantile diarrhoea disease in infants and young children below five years worldwide. rotavirus is associated with high cases of morbidity and mortality and it is estimated that up to 650,000 deaths in young children occur annually in the less developed countries and approximately 150,000-200,000 deaths occur in africa alone. | 2006 | 17089495 |
| association of rotavirus viroplasms with microtubules through nsp2 and nsp5. | rotavirus replication and virus assembly take place in electrodense spherical structures known as viroplasms whose main components are the viral proteins nsp2 and nsp5. the viroplasms are produced since early times after infection and seem to grow by stepwise addition of viral proteins and by fusion, however, the mechanism of viropiasms formation is unknown. in this study we found that the viroplasms surface colocalized with microtubules, and seem to be caged by a microtubule network. moreover i ... | 2006 | 17072471 |
| [evaluation of seven immunochromatographic assays for the rapid detection of human rotaviruses in fecal specimens]. | seven commercially available immunochromatographic assays were tested for the rapid detection of group a rotaviruses in fecal samples compared to a enzyme immunoassay (argene). detection of rotaviruses in 80 elisa positive frozen stool samples showed rates superior to 90% for three reagents (rota strip (cypress diagnostics), 98.8%; rotascreen (microgen), 95.0%; vikia rota/adeno (biomérieux), 92.5%); from 82.5% to 88.8% for three others (diarlex with centrifugation (orion diagnostica), 88.8%; com ... | 2007 | 17049423 |
| evidence for independent segregation of the vp6- and nsp4- encoding genes in porcine group a rotavirus g6p[13] strains. | molecular characterization of two porcine group a rotavirus strains (hp113 and hp140), detected from eastern india, revealed a vp7 closely related to those of human g6p[14] strains, vp4 with a borderline p[13] genotype, and vp6 related to bovine and human sgi strains rather than porcine sgi and/or sgii group a rotaviruses. both strains had nsp4 and nsp5 of porcine origin. therefore, to our knowledge, the present study is the first report of detection of group a rotavirus strains with g6p[13] gen ... | 2007 | 17006597 |
| detection of rare g3p[19] porcine rotavirus strains in chiang mai, thailand, provides evidence for origin of the vp4 genes of mc323 and mc345 human rotaviruses. | among 175 fecal specimens collected from diarrheic piglets during a surveillance of porcine rotavirus (porv) strains in chiang mai, thailand, 39 (22.3%) were positive for group a rotaviruses. of these, 33.3% (13 of 39) belonged to g3p[19], which was a rare p genotype seldom reported. interestingly, their vp4 nucleotide sequences were most closely related to human p[19] strains (mc323 and mc345) isolated in 1989 from the same geographical area where these porv strains were isolated. these p[19] p ... | 2006 | 16988014 |
| genetic characterization of vp3 gene of group a rotaviruses. | we sequenced vp3 gene of four rotavirus strains and examined the diversity of vp3 and its other genetic characteristics in a total of 22 group a rotaviruses. the 22 rotavirus strains were derived from six different host species (human, avian, bovine, equine, simian and porcine). comparison of the partial vp3 peptide sequences (aa 141-294) showed identities ranging from 49 to 99% among different species. the phylogenetic analysis of vp3 sequences revealed segregation according to the species orig ... | 2006 | 16972027 |
| real-time reverse transcription-pcr for detection of rotavirus and adenovirus as causative agents of acute viral gastroenteritis in children. | viral pathogens are the most common cause of gastroenteritis in developed countries. human rotavirus and adenovirus infections are major causes of acute outbreaks and sporadic cases of gastroenteritis, occurring primarily among children less than 2 years of age. patient hospitalization is often required, with enormous infection control implications. this work describes the development of real-time pcr assays for the detection of group f adenovirus, rotavirus a, and rotavirus c from stool specime ... | 2006 | 16954246 |
| molecular characterization of novel g5 bovine rotavirus strains. | group a rotaviruses are a major cause of acute gastroenteritis in young children as well as many domestic animals. the rotavirus genome is composed of 11 segments of double-stranded rna and can undergo genetic reassortment during mixed infections, leading to progeny viruses with novel or atypical phenotypes. the aim of this study was to determine if the bovine group a rotavirus strains kj44 and kj75, isolated from clinically infected calves, share genetic features with viruses obtained from hete ... | 2006 | 16928963 |
| viral gastroenteritis in children hospitalised in sicily, italy. | the aim of the present study was to describe the epidemiologic and clinical characteristics of acute viral gastroenteritis in hospitalised italian children. a total of 215 stool specimens were collected from january to december 2003 from patients hospitalised in palermo for acute diarrhoea. samples were tested for group a rotavirus, astrovirus, adenovirus, norovirus, enteropathogenic bacteria, and parasites. rotaviruses, mostly belonging to types g1-g4, were detected in 25.1% of samples, astrovi ... | 2006 | 16917775 |
| rotavirus genome replication and morphogenesis: role of the viroplasm. | the rotaviruses, members of the family reoviridae, are icosahedral triple-layered viruses with genomes consisting of 11 segments of double-stranded (ds)rna. a characteristic feature of rotavirus-infected cells is the formation of large cytoplasmic inclusion bodies, termed viroplasms. these dynamic and highly organized structures serve as viral factories that direct the packaging and replication of the viral genome into early capsid assembly intermediates. migration of the intermediates to the en ... | 2006 | 16909900 |
| assessment of the quantitative real-time polymerase chain reaction using a cdna standard for human group a rotavirus. | nucleic acid amplification techniques are used frequently for rapid diagnosis of viral diseases. in this study, a real-time polymerase chain reaction protocol that uses primers specific for the viral vp4 gene and the commercial sybr green reagent were evaluated for the quantitative measurement of human rotavirus (hrv) rna in human stool specimens. sybr green i detection involved analysis of the melting temperature of the pcr product and measurement of fluorescence at the optimum temperature. the ... | 2006 | 16890998 |
| structure-function analysis of rotavirus nsp2 octamer by using a novel complementation system. | viral inclusion bodies, or viroplasms, that form in rotavirus-infected cells direct replication and packaging of the segmented double-stranded rna (dsrna) genome. nsp2, one of two rotavirus proteins needed for viroplasm assembly, possesses ntpase, rna-binding, and helix-unwinding activities. nsp2 of the rotavirus group causing endemic infantile diarrhea (group a) was shown to self-assemble into large doughnut-shaped octamers with circumferential grooves and deep clefts containing nucleotide-bind ... | 2006 | 16873255 |
| detection and molecular characterization of group a rotavirus from hospitalized children in rio de janeiro, brazil, 2004. | rotavirus is a major cause of infantile acute diarrhea, causing about 440,000 deaths per year, mainly in developing countries. the world health organization has been recommending the assessment of rotavirus burden and strain characterization as part of the strategies of immunization programs against this pathogen. in this context, a prospective study was made on a sample of 134 children with acute diarrhea and severe dehydration admitted to venous fluid therapy in two state hospitals in rio de j ... | 2006 | 16862325 |
| rotavirus diarrhea in children and adults in a southern city of brazil in 2003: distribution of g/p types and finding of a rare g12 strain. | between may and august in 2003, a total of 251 fecal samples were collected from children and adults with diarrhea (5 inpatients and 246 outpatients) at a private hospital in the city of ponta grossa, the state of paraná, brazil. group a rotavirus was detected in 71 of 251 (28.3%) specimens: 55 (77.5%) from children under 5 years of age and 16 (22.5%) from individuals aged 6-72 years. all 71 strains exhibited a "long" rna pattern when analyzed by page. sixty-one positive samples that yielded eno ... | 2006 | 16847962 |
| g-serotypes of group a rotaviruses in pilsen region (czechia). | a total of 260 feces samples from children with confirmed rotavirus infection collected during 1999-2002 were serotyped, using enzymoimmunoassay with vp7 specific monoclonal antibodies for g1-g4 serotypes. the serotypes were identified in 185 feces, i.e. 71.2 %. individual serotypes occurred in 43, 2, 16 and 2 %; 8 % samples reacted with 2 type-specific monoclonal antibodies. the g1 serotype was prevalent over the whole period. the g3 type occurred with a statistically higher significance in chi ... | 2006 | 16821723 |
| molecular epidemiology of group a rotaviruses in water sources and selected raw vegetables in southern africa. | group a rotaviruses (rvs) are the most important cause of acute viral gastroenteritis in infants and young children. in this study raw and treated drinking water supplies at plants in two geographic areas, as well as selected irrigation water and corresponding raw vegetables in three regions of southern africa, were screened for the presence of rvs using molecular techniques. group a rvs were detected in 11.8% of partially treated and 1.7% of finally treated drinking water samples and in 14% of ... | 2006 | 16820443 |
| viral gastroenteritis and genetic characterization of recombinant norovirus circulating in eastern russia. | from november 2003 to march 2004 a total of 100 fecal specimens from infants and children with acute gastroenteritis in the city of birobidzhan, eastern russia were tested for the presence of diarrheal viruses by rt-multiplex pcr. of these, 74 fecal specimens were positive for diarrheal viruses and this represented 74%. among the diarrheal viruses detected, group a rotavirus was the most prevalent (67%; 67 of 100), followed by norovirus (4%; 4 of 100), group c rotavirus (1%, 1 of 100), sapovirus ... | 2006 | 16812951 |
| an outbreak of rotavirus infection among adults in an institution for rehabilitation: long-term residence in a closed community as a risk factor for rotavirus illness. | an outbreak of group a rotavirus infection resulted in gastroenteritis among disabled adults in an isolated rehabilitation institution in kobe, japan. of the 95 residents, 16 were diagnosed with rotavirus illness. the causative agent was a single strain of typical human group a rotavirus belonging to vp7 serotype g2, vp4 genotype p[4], and nsp4 genotype a. mean duration of stay was significantly longer for residents with rotavirus illness (22.1+/-11.8 years) than for residents without the diseas ... | 2006 | 16798700 |
| multiplex reverse transcription-pcr for rapid differential detection of porcine epidemic diarrhea virus, transmissible gastroenteritis virus, and porcine group a rotavirus. | a novel multiplex reverse transcription polymerase chain reaction (multiplex rt-pcr) that can detect porcine epidemic diarrhea virus (pedv), transmissible gastroenteritis virus (tgev), and porcine group a rotavirus (gar) was developed. the 3 viruses (pedv, tgev, and porcine gar) are major agents in viral enteric diseases of piglets. as the clinical signs of these diseases are similar, including watery diarrhea, differential detection is required for etiologic diagnosis. a mixture of 3 pairs of p ... | 2006 | 16789718 |
| rt-pcr based diagnosis revealed importance of human group b rotavirus infection in childhood diarrhoea. | human group b rotavirus was first identified as causative agent of a large outbreak of severe gastroenteritis affecting more than 1 million people, predominantly adults in china in 1982-1983. in spite of serological evidences for the presence of group b rotavirus in many countries of the world, the virus has been detected only from china, india and bangladesh, where most of the cases were from adults. | 2006 | 16765641 |
| molecular epidemiology of group a rotavirus diarrhea among children in buenos aires, argentina, from 1999 to 2003 and emergence of the infrequent genotype g12. | to examine the epidemiology of rotaviruses in buenos aires, argentina, we screened 1,212 stool samples from children with diarrhea in the southern district of buenos aires from 1999 to 2003. we identified 187 samples (15.4%) that were positive for group a rotavirus by use of antigen enzyme-linked immunosorbent assay. among these specimens, 112 were available for typing: 93 (83.0%) were single-type infections, 9 (8.0%) were mixed-type infections with more than one g or p type, and 10 (8.9%) were ... | 2006 | 16757596 |
| isolation and characterization of a new simian rotavirus, yk-1. | to effectively analyze the requirements for protection to rotavirus infection, a reliable animal model that reasonably mimics infection and disease in humans is needed. a requirement for an effective animal model is the availability of appropriate rotavirus stocks for challenge. | 2006 | 16737519 |
| human rotavirus serotype g9, são paulo, brazil, 1996-2003. | a total of 3,101 fecal specimens were collected during an 8-year survey for rotavirus infection in são paulo, brazil. group a rotavirus was detected in 774 (25.0%) specimens. of these, 431 strains (55.7%) were analyzed for g and p types by reverse transcription-polymerase chain reaction; g1 was the predominant serotype (68.2%), followed by g9 (17.2%), g4 (6.3%), g2 (1.2%), g3 (0.7%), mixed infection (1.8%), and untypeable (4.6%). both rotavirus g and p types could be established in 332 strains ( ... | 2006 | 16707053 |
| a large multi-pathogen waterborne community outbreak linked to faecal contamination of a groundwater system, france, 2000. | a large waterborne outbreak of infection that occurred during august 2000 in a local community in france was investigated initially via a rapid survey of visits to local physicians. a retrospective cohort study was then conducted on a random cluster sample of residents. of 709 residents interviewed, 202 (28.5%) were definite cases (at least three liquid stools/day or vomiting) and 62 (8.7%) were probable cases (less than three liquid stools/day or abdominal pain). those who had drunk tap water h ... | 2006 | 16700706 |
| expression and purification of rotavirus proteins nsp5 and nsp6 in escherichia coli. | rotaviruses are one of the worldwide leading causes of gastroenteritis in children under 5 yr old. the rotavirus nonstructural nsp5 is a phosphoprotein implicated in viroplasms formation, whereas nsp6 could have a possible regulatory role of nsp5. it has been reported that n- and c-termini of nsp5 are important for their function. however, no structural information on nsp5 and nsp6 proteins is available. because a high amount of protein is required for structural analysis, efficient expression s ... | 2006 | 16679520 |
| changing pattern of human group a rotaviruses: emergence of g12 as an important pathogen among children in eastern india. | rotavirus genotypes, g1-g4 and g9 are associated with childhood diarrhoea throughout the world. in our previous study, we detected g1, g2, g4 and three g12 strains from kolkata, india. | 2006 | 16679056 |
| g8 rotavirus strains isolated in the democratic republic of congo belong to the ds-1-like genogroup. | several g8p[6] and g8p[8] rotavirus strains were isolated from hospitalized patients in the democratic republic of congo in 2003. to investigate their overall genomic relatedness and to determine to which genogroup they belonged, the complete genomes of strains drc88 (g8p[8]) and drc86 (g8p[6]) were determined. genomic comparison of these two african g8 strains revealed that 10 out of their 11 gene segments, except for vp4, were nearly identical (>98.9% identical at the nucleotide level), sugges ... | 2006 | 16672410 |
| rare group a rotavirus p[9]g3 isolated in nara prefecture, japan. | 2006 | 16632920 | |
| outbreak of sapovirus infection among infants and children with acute gastroenteritis in osaka city, japan during 2004-2005. | one hundred and twenty five fecal specimens were collected from sporadic cases of acute gastroenteritis in a pediatric clinic in osaka city, japan from july 2004 to june 2005 and tested for the presence of rotavirus, norovirus, sapovirus, astrovirus, and adenovirus by rt-multiplex pcr. among diarrheal viruses detected, norovirus was the most prevalent (19.2%, 24 of 125), followed by group a rotavirus (18.4%, 23 of 125), astrovirus (1.6%, 2 of 125), and adenovirus (0.8%, 1 of 125), respectively. ... | 2006 | 16628578 |
| molecular characterization of a porcine group a rotavirus strain with g12 genotype specificity. | a porcine group a rotavirus strain (ru172) was detected and molecularly characterized during a surveillance study conducted for rotavirus infection in a pig farm located in a suburban area of kolkata city, india. the g12 genotype specificity of ru172 was revealed by pcr-based genotyping assays following addition of a g12 type-specific primer (designed in our laboratory to pick up g12 isolates from field samples) and was confirmed by sequence analysis of the vp7-encoding gene. the ru172 strain ex ... | 2006 | 16502286 |
| comparative molecular characterization of gene segment 11-derived nsp6 from lamb rotavirus llr strain used as a human vaccine in china. | sequence-length polymorphism is known for rotavirus genetic segment 11 (encodes non-structural protein, nsp6). with the exception of 11 strains that have the coding potential for a 98-residue nsp6, majority of the strains have the potential for a 92-residue nsp6. in nine strains, the coding potential for this protein is even shorter. this report focuses on the nsp6 gene nucleotide sequence of lanzhou lamb rotavirus (llr) strain and its comparative molecular characterization. the llr strain is a ... | 2006 | 16492399 |