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the histology and histopathology of the dental innervation: (section of odontology).the presence of a perivascular neural plexus in the periodontal membrane suggests that the dental structures have both sensory and autonomic nerve supply. the fibres described by mummery are unaffected by section of the inferior dental nerve although there is marked degeneration in all demonstrable nerves. nerve-fibres have been observed describing simple and complex looping in the odontogenetic zone and others which, running tangentially between the odontoblast layer and the dentine, form a ver ...193919992023
gorham-stout syndrome: a rare clinical entity and review of literature.gorham-stout syndrome is a very rare bone condition of unknown etiology. it is characterized by the spontaneous onset of bone resorption. bones that previously appeared normal begin to resorb, partially or completely. sometimes only a thin shell of cortical bone remains, and there is usually a little replacement by fibrous tissue. this process can continue for years but may stop spontaneously. bone loss can occur in one bone or spread to soft tissue and adjacent bones. although the disease may s ...201019969488
purification and biochemical characterization of polygalacturonase produced by penicillium expansum during postharvest decay of 'anjou' pear.a polygalacturonase (pg) was extracted and purified from decayed tissue of 'anjou' pear fruit inoculated with penicillium expansum. ammonium sulfate precipitation, gel filtration, and cation exchange chromatography were used to purify the enzyme. both chromatographic methods revealed a single peak corresponding to pg activity. pg enzyme activity from healthy and wounded pear tissue was undetectable, which supports the claim that the purified pg is of fungal origin. the purified enzyme had a mole ...201019968548
plasma vegf determination in disseminated lymphangiomatosis-gorham-stout syndrome: a marker of activity? a case report with a 5-year follow-up.disseminated lymphangiomatosis and gorham-stout disease are being considered as two forms of a single rare disease, characterized by a proliferation of lymphatic vessels, triggered by lymphangiogenic factors. there is no biological marker of the disease. plasma vegf might be a useful tool since the recent demonstration of its pivotal role in the mechanism of this disease. a 45-year-old woman with a history of disseminated lymphangiomatosis involving mediastinum, retroperitoneum, spleen and syste ...201019931435
determination of the insecticide imidacloprid in fruit juices using micellar high-performance liquid chromatography.a simple and reliable hplc method was developed to determine imidacloprid, a chloronicotinyl insecticide that has a highly specific affinity to the nicotinic acetylcholine receptor of insects, above its permissible limit of consumption in fruit juices (orange, apple, and a mixture of pineapple and pear). samples were injected directly into a kromasil c18 column, without any pretreatment step, using the micellar mobile phase 0.10 m sodium dodecyl sulfate and 2.5% (v/v) propanol buffered at ph 7 a ...201219916392
clostridium arbusti sp. nov., an anaerobic bacterium isolated from pear orchard soil.an obligately anaerobic, gram-positive, spore-forming bacterial strain, designated sl206(t), was isolated from pear orchard soils. strain sl206(t) cells were straight or slightly curved rods, with motility by peritrichate flagella. cell walls contained meso-diaminopimelic acid; wall sugars were glucose, rhamnose and mannose. the major fatty acids were c(16 : 0), c(18 : 1)omega9c and summed feature 10 (containing c(18 : 1)omega11c/9t/6t). api 20a reactions were negative for oxidase, catalase and ...201019915114
quantitative detection of pear-pathogenic stemphylium vesicarium in orchards.abstract isolates of stemphylium vesicarium causing brown spot of pear can be distinguished from nonpathogenic isolates of s. vesicarium from pear or from other hosts on the basis of distinctive amplified fragment length polymorphism fingerprinting profiles. dna fragments specific for isolates pathogenic to pear were identified and a quantitative polymerase chain reaction (pcr) was developed on the sequence from one of these specific dna loci. this taqman pcr has a high sensitivity with a dynami ...200919900004
[cystoscopy in a heifer with rupture of a patent urachus].this case report describes the clinical, ultrasonographic and cystoscopic findings and treatment in a two-year-old swiss braunvieh heifer with rupture of a patent urachus. the lead signs in the seven-month-pregnant heifer were markedly abnormal general condition and demeanour and a pear-shaped abdomen. the heifer had severe azotaemia, and abdominal ultrasonography revealed ascites, which was diagnosed as uroperitoneum based on an elevated creatinine level in the fluid. a patent urachus was ident ...200919885799
the nature of the growth rate.1. the growth rate of organisms may be considered as a chemical reaction which gives the mature organism as its end-product. the organism grows at a definite rate which is, at any moment, proportional to the amount of growth yet to be made. 2. shoots of young pear trees measured at weekly intervals during the growing season showed a rate similar to that of an autocatalytic reaction. 3. young walnut trees showed distinct cycles of growth in a single season, but the growth in each cycle proceeded ...192019871830
determination and confirmation of nicotinic acid and its analogues and derivates in pear and apple blossoms using high-performance liquid chromatography-diode array-electrospray ionization mass spectrometry.erwinia amylovora causes fire blight, a serious disease of apple and pear. the bacterial pathogen colonizes the flower stigma and hypanthium, where it multiplies and then invades through natural openings (nectarthodes). e. amylovora requires nicotinic acid as growth factor, and competition for nicotinic acid is being explored as a novel biocontrol strategy. the ability of e. amylovora to substitute nicotinic acid with analogues or derivates as growth factors has not been investigated yet. furthe ...200919835358
radiological and ct findings in a extensive upper-limb involvement of gorham's disease: a case report.the gorham-stout syndrome (gorham's massive osteolysis) is a rare condition in which spontaneous, progressive resorption of bone occurs. the etiology is poorly understood.200919829979
diuretic and antioxidant effects of cacti-nea, a dehydrated water extract from prickly pear fruit, in rats.dehydrated extract of the prickly pear fruit opuntia ficus indica, cacti-nea, was evaluated for its chronic diuretic and antioxidant effects in wistar rats. cacti-nea was orally administered daily for seven days at the dose of 240 mg/kg/day. a positive group was orally treated with hydrochlorothiazide at the dose of 10 mg/kg/day and a control group with vehicle. daily measurements of body weight, urine volume, and concentration of sodium, potassium and uric acid in urine were performed for each ...201019777503
genotypic comparison of pantoea agglomerans plant and clinical strains.pantoea agglomerans strains are among the most promising biocontrol agents for a variety of bacterial and fungal plant diseases, particularly fire blight of apple and pear. however, commercial registration of p. agglomerans biocontrol products is hampered because this species is currently listed as a biosafety level 2 (bl2) organism due to clinical reports as an opportunistic human pathogen. this study compares plant-origin and clinical strains in a search for discriminating genotypic/phenotypic ...200919772624
update on comparative genome mapping between malus and pyrus.comparative genome mapping determines the linkage between homologous genes of related taxa. it has already been used in plants to characterize agronomically important genes in lesser studied species, using information from better studied species. in the maloideae sub-family, which includes fruit species such as apple, pear, loquat and quince, genome co-linearity has been suggested between the genera malus and pyrus; however map comparisons are incomplete to date.200919747407
functional analysis of the n terminus of the erwinia amylovora secreted effector dspa/e reveals features required for secretion, translocation, and binding to the chaperone dspb/f.dspa/e is a type iii secreted effector protein required for pathogenicity in the apple and pear pathogen erwinia amylovora, and dspb/f is a small chaperone protein involved in dspa/e secretion. while the secretion and translocation signals of many type iii secretion effector proteins in human enteric pathogens have been characterized extensively, relatively little is known about the translocation requirements of many effectors in plant pathogens, including large dspe-like proteins. in this study ...200919737101
endodontic management of horizontally placed molars after gunshot injury to mandible: a case report.a 37-year-old man reported to our department with the history of gunshot injury to the mandible 15 years before. his anterior mandible had been resected earlier and bone graft was seen. intraoral examination of lower jaw revealed 4 remaining mandibular molars. these teeth were severely rotated such that they lay horizontal with respect to the mandibular base. preoperative dentascan spiral computerized tomography (ct) of the patient revealed obliteration of the mesial canals of the mandibular rig ...200919734072
description of cryptosporiopsis kienholzii and species profiles of neofabraea in major pome fruit growing districts in the pacific northwest usa.the objectives of this study were i) to give a taxonomic description of a fungus phylogenetically related to neofabraea and assign the name cryptosporiopsis kienholzii to this fungus, ii) to expand previous neofabraea species profiles from infected apple and pear fruit collected from major pome fruit production districts in oregon and washington, and iii) to determine the sensitivity of neofabraea alba, neofabraea malicorticis, neofabraea perennans, and c. kienholzii to a range of fungicides. a ...200919733662
complex combined vascular malformations and vascular malformation syndromes affecting the extremities in children.complex combined vascular malformations affecting extremities are an interesting group of vascular malformations because, in addition to the vascular channel anomalies present, they can be associated with other tissue changes and sometimes altered limb growth. at present, magnetic resonance imaging is the gold standard imaging tool to evaluate such complex conditions in children because of its inherent tissue specificity and vascular capabilities that enables characterization of tissues and the ...200919724993
influence of post-harvest treatments with fludioxonil and soy lecithin co-application in controlling blue and grey mould and fludioxonil residues in coscia pears.the residue levels of fludioxonil (flu) were determined in coscia pear following a 1-, 2- or 4-min dip in an aqueous mixture of flu containing 300 or 100 mg l(-1) (active ingredient, a.i.) at 20 and 50 degrees c, respectively, with or without 2% soy lecithin. the efficacy of heat treatment with water and flu mixtures was investigated on artificially inoculated pears for the control of post-harvest decay caused by blue (penicillium expansum link) and grey (botrytis cinerea pers. ex fr.) mould. tr ...200919680873
light, electron microscopy and histopathology of myxobolus salminus n. sp., a parasite of salminus brasiliensis from the brazilian pantanal.in this report, we describe the morphology and histopathology of myxobolus salminus n. sp., a parasite of the gill filaments of wild salminus brasiliensis (dourado) from the brazilian pantanal. the small polysporic plasmodia were approximately 100 microm in diameter and the development was asynchronous. the mature spores were oval to pear shaped and had a smooth wall. the spore measurements were (mean+/-s.d., with range in parentheses): length 10.1+/-0.4 microm (9.6-10.5), width 6.1+/-0.4 microm ...200919640650
seasonal occurrence and impact of halyomorpha halys (hemiptera: pentatomidae) in tree fruit.halyomorpha halys is an introduced stink bug species from asia that is spreading throughout the mid-atlantic united states. it is native to south korea, japan, and eastern china, where it is an occasional pest of tree fruit, including apple and pear. cage experiments with adults placed on apple and peach during critical plant growth stages demonstrate that it can cause damage to developing fruit during mid- and late season growth periods and that feeding occurs on all regions of the fruit. feedi ...200919610429
development of an improved isolation approach and simple sequence repeat markers to characterize phytophthora capsici populations in irrigation ponds in southern georgia.phytophthora capsici, the causal agent of phytophthora blight, is a major concern in vegetable production in georgia and many other states in the united states. contamination of irrigation water sources by p. capsici may be an important source of inoculum for the pathogen. a simple method was developed in this study to improve the efficiency of recovering p. capsici from fruits used as baits in irrigation ponds. in contrast to direct isolation on agar plates, infected fruit tissues were used to ...200919581483
fatty acid composition in lipid fractions lengthwise the mycelium of mortierella isabellina and lipid production by solid state fermentation.this paper investigates the correlation between mycelial age and fatty acid biosynthesis. the correlation was investigated by analyzing the lipid composition lengthwise the mycelium of the oleaginous fungus mortierella isabellina, a potential producer of gamma-linolenic acid (gla). young mycelia were rich in polar lipids (glycolipids plus sphingolipids and phospholipids), while neutral lipid content increased in aged mycelia. in young mycelia, each polar lipid fraction contained almost 40% (w/w) ...200919574039
effect of aqueous extracts of alligator pear seed (persea americana mill) on blood glucose and histopathology of pancreas in alloxan-induced diabetic rats.effects of aqueous extract of alligator pear seed on normal and alloxan-induced diabetic rats were investigated in 6 groups of rats (5 rats per group). test groups were made diabetic with intra-peritoneal injection of alloxan and treated with 300 mg and 600 mg/kg body weight of alligator pear seed extract. two non-diabetic groups were also administered with 300 mg and 600 mg/kg body weight extract. the levels of blood glucose were examined in all 6 experimental groups. in diabetic rats, blood gl ...200919553173
mycobased synthesis of silver nanoparticles and their incorporation into sodium alginate films for vegetable and fruit preservation.biosynthesis of silver nanoparticles using trichoderma viride and their incorporation into sodium alginate for vegetable and fruit preservation has been demonstrated in this study. aqueous silver (ag(+)) ions when exposed to the filtrate of t. viride are reduced in solution. these extremely stable silver nanoparticles were characterized by means of uv-vis spectrophotometer, ftir, tem, and eds. the nanoparticles exhibit maximum absorbance at 421 nm in the uv spectrum. the presence of proteins was ...200919552418
spermidine levels are implicated in heavy metal tolerance in a spermidine synthase overexpressing transgenic european pear by exerting antioxidant activities.to verify whether spermidine synthase (spds) can confer long-term multi-heavy metal tolerance, in vitro shoots of a transgenic european pear (pyrus communis l. 'ballad') line #32 overexpressing apple spds (mdspds1), as well as a wild type (wt) line, were subjected to stress using either cdcl(2), pbcl(2), zncl(2), or a combination thereof. based on either shoot height increment or fresh weight and morphological changes upon heavy metal stress, the performance of the transgenic line #32 was better ...201019544002
erwinia amylovora strains isolated in romania from outbreaks of fire blight disease: phenotypic characterization.the fire blight disease was described for the first time in romania, in 1992. since then by continuous spreading, this disease has caused severe damages of the fruit trees production, particularly of the pear and quince orchards in different regions of the country, being advantaged by certain weather conditions (high temperatures and humidity). an epidemiological surveillance of this disease that was spreading over different regions of the country, has been instituted since 2002. during the year ...200819496475
evidence for a photoprotective function of low-temperature-induced anthocyanin accumulation in apple and pear peel.the light requirement and low-temperature stimulation of anthocyanin synthesis in peel of apple (malus domestica) and pears (pyrus communis) and the presence of anthocyanins in immature fruits are not congruent with a visual function in dispersal. we hypothesized that anthocyanins afford photoprotection to peel during low-temperature-induced light stress and that the protection is not a fortuitous side-effect of light absorption by anthocyanin. the extent of photoinhibition at harvest and after ...200919493306
[clinical features of gorham-stout syndrome and literature review].to describe the clinical presentations, radiographic findings and histological pathology of bones, diagnosis, treatment options and prognosis for patients with gorham-stout syndrome (gss).200919484972
systems level analysis of two-component signal transduction systems in erwinia amylovora: role in virulence, regulation of amylovoran biosynthesis and swarming motility.two-component signal transduction systems (tcsts), consisting of a histidine kinase (hk) and a response regulator (rr), represent a major paradigm for signal transduction in prokaryotes. tcsts play critical roles in sensing and responding to environmental conditions, and in bacterial pathogenesis. most tcsts in erwinia amylovora have either not been identified or have not yet been studied.200919470164
screening of antiproliferative effect of aqueous extracts of plant foods consumed in méxico on the breast cancer cell line mcf-7.we evaluated the antiproliferative effect of aqueous extracts of 14 plant foods consumed in mexico on the breast cancer cell line mcf-7. the plant foods used were avocado, black sapote, guava, mango, prickly pear cactus stems (called nopal in mexico, cooked and raw), papaya, pineapple, four different cultivars of prickly pear fruit, grapes and tomato. β-carotene, total phenolics and gallic acid contents and the antioxidant capacity, measured by the ferric reducing/antioxidant power and the 2,2-d ...200919468947
role of rpos in stress tolerance and environmental fitness of the phyllosphere bacterium pseudomonas fluorescens strain 122.bacteria living epiphytically on aerial plant surfaces encounter severe and rapidly fluctuating environmental conditions, and their capacity to withstand environmental stress contributes to epiphytic fitness. the stationary phase sigma factor rpos is a key determinant in stress response of gram-negative bacteria, including pseudomonas spp. this study focused on the role of rpos in stress response and epiphytic fitness of pseudomonas fluorescens strain 122 on aerial plant surfaces. rpos had a sig ...200919453227
stress tolerance and environmental fitness of pseudomonas fluorescens a506, which has a mutation in rpos.establishment of suppressive populations of bacterial biological control agents on aerial plant surfaces is a critical phase in biologically based management of floral diseases. periodically, biocontrol agents encounter inhospitable conditions for growth on plants; consequently, tolerance of environmental stresses may contribute to their fitness. in many gram-negative bacteria, including strains of pseudomonas spp., the capacity to survive environmental stresses is influenced by the stationary p ...200919453226
gorham-stout syndrome with chylothorax: successful remission by interferon alpha-2b.gorham-stout syndrome is a rare disease and most often recognized in children and young adults. chylothorax is a serious complication of gorham-stout syndrome. the treatment of either gorham-stout syndrome or chylothorax is still a dilemma. we described a 9-year-old girl with gorham-stout syndrome and chylothorax who was admitted to our hospital because of dyspnea, orthopnea, and bone pain, and treated with interferon alpha-2b.200919434689
first report on the presence of fire blight resistance in linkage group 11 of pyrus ussuriensis maxim.fire blight, caused by the gram-negative bacterium erwinia amylovora (burrill) winslow et al., is a dangerous disease of pome fruits, including pear. a pear breeding program for fire blight resistance was initiated in 2003 at the department of pomology, warsaw university of life sciences, poland. since several asian species are considered to be potential sources of resistance to fire blight, the susceptible pyrus communis 'doyenne du comice' was crossed with the resistant p. ussuriensis. the f1 ...200919433906
fine structure of the bird parasites trichomonas gallinae and tetratrichomonas gallinarum from cultures.the trophozoites of trichomonas gallinae and tetratrichomonas gallinarum were studied by means of light and electron microscopy after cloning and cultivating them axenically. t. gallinae trophozoites varied in shape reaching from ovoidal to pyriform and had a size of about 7-11 microm. they were provided with four free flagella and a fifth recurrent one, which did not become free at the posterior pole. the nucleus was ovoid, had a size of about 2.5-3 microm, and was situated closely below the ba ...200919421777
a familial case of trichorhinophalangeal syndrome type i.trichorhinophalangeal type i (trps i) is a rare autosomal dominant disorder characterized by variable clinical expression of sparse and slow-growing hair, pear-shaped nose, elongated philtrum, and bone deformities, including cone-shaped epiphyses of the phalanges and short stature. we describe three members of a family who consulted us because of slow-growing scalp hair with craniofacial and radiological features typical of trps i.200919419465
specific detection and quantification of the phytopathogenic agent 'candidatus phytoplasma prunorum'.'candidatus phytoplasma prunorum' is a wall-less bacterium associated with european stone fruit yellows (esfy), a severe disease of prunus spp. (mainly apricot and japanese plum trees). it can be spread by one insect vector, cacopsylla pruni, and by the trade of infected material. the availability of pcr-based methods allowing a sensitive and specific detection of 'ca. p. prunorum' is crucial for this phytoplasma because, at present, it is uncultured and cannot be detected serologically. we deve ...200919401232
population structure of potebniamyces pyri in the u.s. pacific northwest and evidence of outcrossing inferred with sequence-characterized amplified region markers.potebniamyces pyri is the cause of phacidiopycnis rot of d'anjou pear, which is grown primarily in washington and oregon. to estimate the population structure of p. pyri, 146 single-spore isolates were sampled from five major pear-production areas and scored for variation at eight sequence-characterized amplified region (scar) loci. significant genetic differentiation was detected among five subpopulations and a total of 54 multilocus genotypes were identified, with significant genotypic diversi ...200919351249
gorhams disease: vanishing bone syndrome.gorhams disease, also known as massive osteolysis or vanishing bone disease is an extremely rare bone disease. it is characterized by angiomatosis with adjacent bone resorption. we report an 8-years old boy with the disease who was managed successfully with alpha 2b interferon therapy.200919346574
erwinia amylovora: modern methods for detection and differentiation.erwinia amylovora is the causative agent of fire blight, a very destructive disease of numerous members of the rosaceae. the primary route of infection for host species, including commercially grown apple and pear, is the newly opened blossom. susceptibility of flowers to infection for only a few days creates narrow window for infection. not surprisingly, the risk of disease is related to e. amylovora population size. as a result, methods that supply quick, accurate and sensitive quantification ...200919301751
vanishing bone disease: review and case reports.vanishing bone disease is a rare idiopathic disease, leading to extensive loss of bony matrix, replaced by proliferating thin-walled vascular channels and fibrous tissue. there are >191 cases reported in the english literature. gorham and stout made the first overview of the disease in 1955 and they first presented 24 cases known at that time. the etiology remains speculative, the prognosis unpredictable, and effective therapy still unknown. the disease can be monostotic or polyostotic although ...200819292231
progressive gorham disease of the forearm.gorham's-stout disease is a rare but potentially debilitating disease consisting of massive bone osteolysis and bone resorption associated with vascular proliferation and increased osteoclastic activity. although it can present in a wide variety of forms, it typically involves bones formed by intramembranous ossification such as the skull, pelvis, and scapula. it can occur spontaneously or after trauma. most cases are monofocal and resolved spontaneously, although there are reports of multifocal ...200819292225
host suitability of eight prunus spp. and one pyrus communis rootstocks to pratylenchus vulnus, p. neglectus, and p. thornei.the effects of pratylenchus vulnus on rootstocks of eight commonly used prunus spp. and one pyrus communis were evaluated under greenhouse conditions during a 15-month period. in a first experiment, two almonds (moncayo and garrigues), one peach (gf-305), and two peach-almond hybrids (gf-677 and adafuel) inoculated with 2,000 nematodes per plant proved to be good hosts of p. vulnus. highest (p < 0.05) numbers of nematodes per gram of fresh root weight were recovered from adafuel and gf-677. root ...199119283165
meloidogyne lusitanica n. sp. (nematoda: meloidogynidae), a root-knot nematode parasitizing olive tree (olea europaea l.).a root-knot nematode from portugal, meloidogyne lusitanica n. sp., is described and illustrated from specimens obtained from olive trees (olea europaea l.). females of the new species have a characteristic perineal pattern with medium to high trapezoidal dorsal arch with distinct punctuations in the tail terminus area. the excretory pore is located posterior to the stylet, about 1.5-2.5 stylet lengths from the anterior end. the stylet is 17.1 mum long with pear-shaped knobs. males have a rounded ...199119283115
host range of a population of pratylenchus vulnus in commercial fruit, nut, citrus, and grape rootstocks in spain.in a host-range study carried out under greenhouse conditions, a total of 37 commercial fruit tree, grape, and citrus rootstocks were tested for their reaction to a population of the lesion nematode, pratylenchus vulnus, in spain. twenty-five rootstocks had a pf/pi > 1.5. these included almond (desmayo rojo, 1143), apple (em-9, em-106), avocado (hass), cherry (santa lucia 64, camil, m x m 14, masto de montafiana), grape (41-b, fercal, ritcher 110), hazelnut (pauetet), loquat (nadal), peach (mont ...199219283047
function of genes encoding acyl-coa synthetase and enoyl-coa hydratase for host-selective act-toxin biosynthesis in the tangerine pathotype of alternaria alternata.the tangerine pathotype of alternaria alternata produces host-selective act-toxin and causes alternaria brown spot disease. sequence analysis of a genomic cosmid clone identified a part of the actt gene cluster and implicated two genes, actt5 encoding an acyl-coa synthetase and actt6 encoding an enoyl-coa hydratase, in the biosynthesis of act-toxin. genomic southern blots demonstrated that both genes were present in tangerine pathotype isolates producing act-toxin and also in japanese pear patho ...200919271978
[online detection of soluble solids content of pear by near infrared transmission spectrum].the research was to detect soluble solids content (ssc) of pear online by near infrared transmission spectrum. the movement speed of pear was 0.5 m x s(-1) the power of light source was 300 w, and semi-transmission was used to collect the spectrum of pears. the total experiment samples were 187 pears, with a calibration set of 147 pears and a validation set of 40 pears. partial least squares (pls) and principal component regression (pcr) technique were used to develop the calibration model for o ...200819271484
survey of crop losses in response to phytoparasitic nematodes in the united states for 1994.previous reports of crop losses to plant-parasitic nematodes have relied on published results of survey data based on certain commodities, including tobacco, peanuts, cotton, and soybean. reports on crop-loss assessment by land-grant universities and many commodity groups generally are no longer available, with the exception of the university of georgia, the beltwide cotton conference, and selected groups concerned with soybean. the society of nematologists extension committee contacted extensio ...199919270925
differential responses in pear and quince genotypes induced by fe deficiency and bicarbonate.most of the studies carried out on fe deficiency condition in arboreous plants have been performed, with the exception of those carried out on plants grown in the field, in hydroponic culture utilizing a total iron depletion growth condition. this can cause great stress to plants. by introducing fe deficiency induced by the presence of bicarbonate, we found significant differences between pyrus communis l. cv. conference and cydonia oblonga mill. ba29 and ma clones, characterized by different le ...200919269060
the study and analysis of the mating behavior and sound production of male cicada psalmocharias alhageos (kol.) (homoptera:cicadidae) to make disruption in mating.psalmocharias alhageos is an important pest of vine in most parts of iran, afghanistan, pakistan, southern areas of russia, turkey and iraq. this cicada is spread in most provinces in iran such as esfahan, hamedan, qazvin, markazi, lorestan, qom, kerman, tehran and kordestan. in addition to vine, this insect damages some other fruit trees, such as apple, sour cherry, quince, peach, pomegranate and pear trees and some non-fruit trees, namely white poplar, ash, elm, eglantine, silk and black popla ...200819266919
sugar profiles and soluble and insoluble dietary fiber contents of fruits in thailand markets.the objective of the present study was to determine sugar and dietary fiber contents in 37 varieties of thai fruits. sugars were determined by high-performance liquid chromatography and dietary fiber values were measured by the enzymatic-gravimetric method. the total sugar (sucrose, fructose and glucose) content ranged from 4.5 g/100 g (strawberry) to 20.3 g/100 g (ripe banana; hawm variety) edible portion. all varieties of ripe banana provided good sources of glucose, fructose and total sugar. ...200919255919
international multidimensional authenticity specification (imas) algorithm for detection of commercial pomegranate juice adulteration.the pomegranate fruit ( punica granatum ) has become an international high-value crop for the production of commercial pomegranate juice (pj). the perceived consumer value of pj is due in large part to its potential health benefits based on a significant body of medical research conducted with authentic pj. to establish criteria for authenticating pj, a new international multidimensional authenticity specifications (imas) algorithm was developed through consideration of existing databases and co ...200919249817
pharmacogenomics of antihypertensive drugs: rationale and design of the pharmacogenomic evaluation of antihypertensive responses (pear) study.selection of antihypertensive therapy is often empiric, and use of genetic information to guide drug therapy selection holds future promise.200919249413
epithelial-mesenchymal transitions in development and disease: old views and new perspectives.the epithelial to mesenchymal transition (emt) is a fascinating phenotypic change that is undertaken by embryonic and adult cells in physiological and pathological conditions, respectively. this change in cell behavior involves the loss of epithelial characteristics and the acquisition of migratory properties. while it has long been established as a fundamental process in the generation of many different embryonic tissues, its significance during tumor progression as an initial determining step ...200919247945
inactivation of listeria monocytogenes in raw fruits by enterocin as-48.the purpose of this study was to determine the effect of enterocin as-48 on listeria monocytogenes cect 4032 in fruits and fruit juice. fruits were contaminated with a l. monocytogenes cell suspension, washed with enterocin as-48 (25 microg/ml) or with sterile distilled water as control, and stored at different temperatures (-20, 6, 15, 22 degrees c). washing treatments significantly inhibited or completely inactivated l. monocytogenes in strawberries, raspberries, and blackberries stored at 15 ...200819244899
the rcs phosphorelay system is essential for pathogenicity in erwinia amylovora.the rcs phosphorelay system is a modified two-component signal transduction system found exclusively in enterobacteriaceae. in this study, we characterized the roles of the rcs system in erwinia amylovora, a highly virulent and necrogenic enterobacterium causing fire blight disease on rosaceous plants. our results showed that rcsb, rcsc, rcsd and rcsbd mutants were non-pathogenic on immature pear fruit. the bacterial growth of these mutants was also greatly reduced compared with that of the wild ...200919236575
high-yield production and characterization of biologically active recombinant aprotinin expressed in saccharomyces cerevisiae.aprotinin is a polypeptide composed of 58 amino acid residues and has a molecular weight of 6512da. the 58 amino acid residues are arranged in a single polypeptide chain, which is cross-linked by three disulfide bridges and folded to form a pear-shaped molecule. to express recombinant aprotinin in saccharomyces cerevisiae, a synthetic gene encoding aprotinin was constructed and fused in frame with the pre-sequence of the s. cerevisiae matalpha1 gene at the cleavage site of signal peptidase. the ...200919233283
efficacy results of insecticides against cydia pomonella, the codling moth, in belgium during the last decade (1998-2007).the codling moth is an economically important top fruit pest, with its major flight from may till august. we give an overview of ten years of results of efficacy field trials against the codling moth cydia pomonella, with a number of commonly used products (diflubenzuron, fenoxycarb, flufenoxuron, indoxacarb, granulosis virus, methoxyfenozide and tebufenozide). all trials were executed according to eppo guidelines. trials were performed in the belgian fruit growing area nearby sint-truiden, on a ...200819226808
susceptibility of ceratitis capitata wiedemann (diptera: tephritidae) to entomopathogenic fungi.the mediterranean fruit fly ceratitis capitata (wiedemann) is one of the most serious pest of fruits in syria and present all year round. this fly is active on different host plants, such as mango, peach, fig, apple, pear, and citrus. the number of generations per year varies with local temperatures and host plant. the objective of this research was: to evaluate the pathogenicity of entomopathogenic fungi on adults of c. capitata under laboratory conditions. flies for experiments were obtained f ...200819226799
scale insects and mealy bugs (homoptera: coccoidea) attacking deciduous fruit trees in the western north coast of alexandria, egypt.this investigation covered a survey of scale insects and mealy bugs infesting ten growing species of deciduous fruit trees in three localities in alexandria govemorate. these localities were merghem, burg el-arab, and el-nahda about 50 km. west of alexandria under both rain-fed and irrigation system conditions. the common inspected fruit trees were fig, white mulberry, pomegranate, apple, pear, apricot, european plum, peach, almond, and persimmon. it was shown that a group of twenty scale insect ...200819226793
the gorham-stout syndrome: one lung ventilation with a bronchial blocker. a case of gorham's disease with chylothorax. 200919207918
modified version of adm1 model for agro-waste application.agro-residues account for a large proportion of the wastes generated around the world. there is thus a need for a model to simulate the anaerobic digestion processes used in their treatment. we have developed model based on adm1, to be applied to agro-wastes. we examined and tested the biodegradability of apple, pear, orange, rape, sunflower, pig manure and glycerol wastes to be used as the basis for feeding the model. moreover, the fractions of particulate cod (x(c)) were calculated, and the di ...200919201603
microscale mechanisms of gas exchange in fruit tissue.* gas-filled intercellular spaces are considered the predominant pathways for gas transport through bulky plant organs such as fruit. here, we introduce a methodology that combines a geometrical model of the tissue microstructure with mathematical equations to describe gas exchange mechanisms involved in fruit respiration. * pear (pyrus communis) was chosen as a model system. the two-dimensional microstructure of cortex tissue was modelled based on light microscopy images. the transport of o(2) ...200919192195
a polyphasic approach assigns the pathogenic erwinia strains from diseased pear trees in japan to erwinia pyrifoliae.bacterial shoot blight of pear in japan (bsbp) is caused by erwinia strains which were formerly associated with the species erwinia amylovora, the causative agent of fire blight. the description of erwinia pyrifoliae as a pear pathogen in korea renewed a possible connection of the pear pathogens in both countries.200919187512
a brief history of vitamin d and cancer prevention.to review the history of vitamin d and its use in cancer prevention.200919185802
cd105/endoglin expression in gorham disease of bone.gorham disease is a rare pathological condition characterised by a proliferation of vascular channels of haematic and lymphatic origin in bone and adjacent soft tissues, which results in a progressive destruction of the involved bone segment.200919181633
quantitative resistance traits and suitability of woody plant species for a polyphagous scarab, popillia japonica newman.the japanese beetle, popillia japonica newman, has an unusually broad host range among deciduous woody plants, yet it feeds only sparingly, or not at all, on certain species in the field. we evaluated beetles' preference, survival over time and fecundity on eight woody plant species historically rated as susceptible or resistant and, after verifying those ratings, tested whether resistance is correlated with so-called quantitative defense traits including leaf toughness, low nutrient content (wa ...200819161699
implications of pathogenesis by erwinia amylovora on rosaceous stigmas to biological control of fire blight.as a prerequisite to infection of flowers, erwinia amylovora grows epiphytically on stigmas, which provide a conducive habitat for bacterial growth. stigmas also support growth of several other bacterial genera, which allows for biological control of fire blight; although, in practice, it is very difficult to exclude e. amylovora completely from this habitat. we investigated the dynamics of growth suppression of e. amylovora by comparing the ability of virulent and avirulent strains of e. amylov ...200919159304
reversible storage of lithium in a rambutan-like tin-carbon electrode.fruity electrodes: a simple bottom-up self-assembly method was used to fabricate rambutan-like tin-carbon (sn@c) nanoarchitecture (see scheme, green sn) to improve the reversible storage of lithium in tin. the mechanism of the growth of the pear-like hairs is explored.200919156791
[adaptation of mycoplasma gallisepticum to unfavorable growth conditions: changes in morphological and physiological characteristics].adaptation of mycoplasma gallisepticum to unfavorable growth conditions results in altered morphological and physiological characteristics of the cells. m. gallisepticum populations in a complete nutrient medium contain pear-shaped vegetative cells (d approximately 0.3 microm; l approximately 0.8 microm) with pronounced polar and cytoskeleton-like structures. such mycoplasma cells are able to induce damage in a bacterial genome, causing an sos response of the test strain (escherichia coli pq37). ...200819137716
pistil-function breakdown in a new s-allele of european pear, s21*, confers self-compatibility.european pear exhibits rnase-based gametophytic self-incompatibility controlled by the polymorphic s-locus. s-allele diversity of cultivars has been extensively investigated; however, no mutant alleles conferring self-compatibility have been reported. in this study, two european pear cultivars, 'abugo' and 'ceremeño', were classified as self-compatible after fruit/seed setting and pollen tube growth examination. s-genotyping through s-pcr and sequencing identified a new s-rnase allele in the two ...200919096853
a new gene for resistance to dysaphis pyri in pear and identification of flanking microsatellite markers.dysaphis pyri is an important aphid pest of european pear (pyrus communis) cultivars, none of which are currently reported to be resistant. in this study, we produced a progeny of the european pear comice crossed with an accession of snow pear (pyrus nivalis) that segregated for resistance to d. pyri in a mendelian fashion, indicating the presence of a major gene, dp-1. following screening of the parents and seedlings with microsatellite markers, cosegregation analysis indicated that dp-1 is fla ...200819088815
effect of a waal mutation on lipopolysaccharide composition, oxidative stress survival, and virulence in erwinia amylovora.erwinia amylovora, the causal agent of fire blight, is an enterobacterial pathogen of rosaceous plants including apple and pear. we have been studying the response of e. amylovora to oxidative stress because, during infection, the bacterium elicits an oxidative burst response in host plants. during the screening of a transposon mutant library for hydrogen peroxide sensitivity, we identified a mutant carrying an insertion in waal, a gene involved in lipopolysaccharide biosynthesis, that was more ...200919076232
mapping glycans onto specific n-linked glycosylation sites of pyrus communis pgip redefines the interface for epg-pgip interactions.polygalacturonase inhibiting proteins (pgips) are members of the leucine rich repeat family of proteins, involved in plant defense against fungal pathogens. pgips exhibit a remarkable degree of specificity in terms of their ability to bind and inhibit their target molecules, the endopolygalacturonases (epgs). this specificity has been attributed for certain epg/pgip combinations to differences in primary sequence, but this explanation is unable to account for the full range of binding and inhibi ...200919072240
in vitro characterisation of koala chlamydia pneumoniae: morphology, inclusion development and doubling time.chlamydia pneumoniae is a common human and animal pathogen associated with upper and lower respiratory tract infections. of the animal c. pneumoniae isolates, the koala nasal isolate (lpcoln) is by far the best genetically characterised. this current study was designed to characterise the morphology and developmental events for the lpcoln isolate, and our results showed several striking in vitro growth differences when compared to the human isolate, ar39. the lpcoln inclusion size and morphology ...200919026498
diffuse lymphangiomatosis--a fatal case with atypical skeletal features.diffuse lymphangiomatosis is a rare idiopathic condition that occurs mostly in children, is characterized by a non-neoplastic proliferation of lymphatic vessels, leading to organ dysfunction, chylous effusions, and death. a closely related condition-the gorham-stout syndrome-is also characterized by lymphangiomatosis and chylous effusions, but also with massive osteolytic changes ("vanishing bone disease"). a 33-year-old woman presented with a 5-year history of worsening chylous effusions and or ...200819011406
patulin causes dna damage leading to cell cycle arrest and apoptosis through modulation of bax, p(53) and p(21/waf1) proteins in skin of mice.patulin (pat), a mycotoxin found in apples, grapes, oranges, pear and peaches, is a potent genotoxic compound. who has highlighted the need for the study of cutaneous toxicity of pat as manual labour is employed during pre and post harvest stages, thereby causing direct exposure to skin. in the present study cutaneous toxicity of pat was evaluated following topical application to swiss albino mice. dermal exposure of pat, to mice for 4 h resulted in a dose (40-160 mug/animal) and time (up to 6 h ...200919000704
in vitro digestion of betalainic foods. stability and bioaccessibility of betaxanthins and betacyanins and antioxidative potential of food digesta.betalains are considered to be bioactive dietary phytochemicals. the stability of betacyanins and betaxanthins from either fresh foods or manufactured products of cactus pear fruit ( opuntia ficus indica l. mill. cv. gialla and rossa) and red beet ( beta vulgaris l. ssp. vulgaris) was assessed in a simulated oral, gastric, and small intestinal digestion and compared with the digestive stability of purified pigments. a minor loss of indicaxanthin, at the gastric-like environment only, and a decre ...200818959410
protective effect of cactus (opuntia ficus indica) cladode extract upon nickel-induced toxicity in rats.the purpose of this study carried out on male wistar rats, was to evaluate the protective effects of regular ingestion of juice from the prickly pear cactus (opuntia ficus indica) cladodes against nickel chloride toxicity. rats were given either normal tap water or water containing 25% of cactus juice for one month. then, rats of each group were injected daily, for 10 days, with either nicl(2) solution (4mg (30micromol)/kg body weight) or with the same volume of saline solution (300mm nacl). sig ...200818950672
molecular variability of the genomes of capilloviruses from apple, japanese pear, european pear, and citrus trees.abstract the 3'-terminal regions of the genomes of apple stem grooving virus (asgv), isolated from apple, japanese pear, and european pear plants, and citrus tatter leaf virus (ctlv), isolated from citrus plants, were amplified by reverse transcription-polymerase chain reaction. the dna products were cloned and sequenced. the results indicated that the asgv isolates from apple, japanese pear, and european pear comprise at least two to four "sequence variants" that differ considerably from each o ...199718945117
aal-toxin-deficient mutants of alternaria alternata tomato pathotype by restriction enzyme-mediated integration.abstract host-specific toxins are produced by three pathotypes of alternaria alternata: am-toxin, which affects apple; ak-toxin, which affects japanese pear; and aal-toxin, which affects tomato. each toxin has a role in pathogenesis. to facilitate molecular genetic analysis of toxin production, isolation of toxin-deficient mutants utilizing ectopic integration of plasmid dna has been attempted. however, the transformation frequency was low, and integration events in most transformants were compl ...199718945069
crab apple blossoms as a model for research on biological control of fire blight.abstract nonseasonal availability of pomaceous flowers could improve laboratory detection and prefield testing of biocontrol agents for fire blight of pear and apple. crab apple was selected as a model because of its high flower productivity on 1-year-old wood, high susceptibility to fire blight, and availability from nurseries. cultivars manchurian and snowdrift were manipulated to bloom once by transferring dormant nursery trees from a cold room to a greenhouse and a second time by defoliating ...199718945005
establishment of bacterial antagonists of erwinia amylovora on pear and apple blossoms as influenced by inoculum preparation.the influence of inoculum preparation on the establishment of bacterial antagonists that suppress fire blight and erwinia amylovora on blossoms was evaluated. aqueous suspensions of pseudomonas fluorescens a506, e. herbicola c9-1r, or e. amylovora 153n were prepared from cells harvested from the surface of an agar medium or from cells that were lyophilized after culture under similar conditions. bacterial suspensions (1 x 10(8) cfu/ml) were sprayed on pear and apple trees at 50% bloom near midda ...199818944901
occurrence of indole-3-acetic acid-producing bacteria on pear trees and their association with fruit russet.abstract a relatively high percentage of epiphytic bacteria on pear leaf and fruit surfaces had the ability to produce indole-3-acetic acid (iaa) in culture media supplemented with tryptophan. while over 50% of the strains produced at least small amounts of iaa in culture, about 25% of the strains exhibited high iaa production as evidenced by both colorimetric and high-performance liquid chromatography analysis of culture supernatants. a majority of the strains that produced high amounts of iaa ...199818944847
effect of nectar on microbial antagonists evaluated for use in control of fire blight of pome fruits.abstract under warm, dry conditions, erwinia amylovora can become established in relatively high populations on apple (malus domestica) or pear (pyrus communis) flower stigmas, and subsequent wet conditions facilitate its movement to the flower hypanthium where infection generally is initiated through the nectarthodes. research on biological control of fire blight has focused mainly on the flower stigma, and knowledge is lacking regarding the effect of nectar on microbial antagonists in the flow ...199918944801
single-strand conformation polymorphism analysis of apple stem grooving capillovirus sequence variants.abstract in an earlier study, we demonstrated that isolates of apple stem grooving capillovirus (asgv) from fruit trees comprise at least two to four sequence variants that differ considerably from each other in nucleotide sequence. in order to characterize the population of sequence variants within a single tree, we applied a combination of an immunocapture reverse transcription-polymerase chain reaction (ic-rt-pcr) and a single-strand conformation polymorphism (sscp) analysis of a nested asymm ...199918944787
purification and biological characterization of host-specific sv-toxins from stemphylium vesicarium causing brown spot of european pear.abstract culture filtrates of a pathogenic isolate (it37) of stemphylium vesicarium, causing brown spot of european pear, induced veinal necrosis only on pear leaves susceptible to the pathogen. two host-specific toxins, sv-toxins i and ii, were purified from culture filtrates of it37 by successively using amberlite xad-2 resin adsorption, cellulose thin-layer chromatography, and high-performance liquid chromatography under three different sets of conditions. susceptible cultivars showed veinal ...199918944740
epiphytic colonization of pear stigmas and hypanthia by bacteria during primary bloom.abstract pear blossoms were sampled during various stages of bloom in 1991 and 1992 from orchards at cashmere, wa, and corvallis and medford, or, for epiphytic populations of culturable bacteria. on stigmatic surfaces, bacteria were isolated from 2 to 32% of blossoms prior to petal expansion and from 47 to 94% of blossoms by petal fall. in general, a lower percentage of hypanthia than stigmas supported bacterial populations. randomly selected bacteria isolated at population levels of >/=10(4) cf ...199918944640
distribution and characterization of akt homologs in the tangerine pathotype of alternaria alternata.abstract the tangerine pathotype of alternaria alternata produces a host-selective toxin (hst), known as act-toxin, and causes alternaria brown spot disease of citrus. the structure of act-toxin is closely related to ak- and af-toxins, which are hsts produced by the japanese pear and strawberry pathotypes of a. alternata, respectively. ac-, ak-, and af-toxins are chemically similar and share a 9,10-epoxy-8-hydroxy-9-methyl-decatrienoic acid moiety. two genes controlling ak-toxin biosynthesis (ak ...200018944496
an extracellular protease of pseudomonas fluorescens inactivates antibiotics of pantoea agglomerans.abstract pseudomonas fluorescens a506 and pantoea agglomerans strains eh252 and c9-1 are biological control agents that suppress fire blight, an important disease of pear and apple caused by the bacterium erwinia amylovora. pseudomonas fluorescens strain a506 suppresses disease largely through competitive exclusion of e. amylovora on surfaces of blossoms, the primary infection court, whereas pantoea agglomerans strains eh252 and c9-1 produce antibiotics that are toxic to e. amylovora. in this st ...200418944458
assessment of environmental factors influencing growth and spread of pantoea agglomerans on and among blossoms of pear and apple.abstract we evaluated effects of both physical and biological components of the environment on growth of pantoea agglomerans on inoculated pear and apple blossoms and on spread of the bacterium to blossoms on non-inoculated trees. the center three rows of 0.35- to 0.5-ha blocks of four pear cultivars and four apple cultivars were sprayed with a suspension of streptomycin-resistant p. agglomerans strain c9-1s (c9-1s) at 20 to 60% and 60 to 90% bloom. cultivars were chosen to create a sequence of ...200018944433
characterization of a viral eps-depolymerase, a potential tool for control of fire blight.abstract a 3.3-kb fragment of genomic dna from bacteriophage phi-ea1h encoding an amylovoran-directed depolymerase lyase was sequenced, and three open reading frames (orfs) were detected. the first orf could encode a lysozyme and the second a holin that may form a pore supporting cell lysis by the lysozyme. the third orf encodes a protein of 657 amino acids and deletion mutation in this dna fragment abolished extracellular polysaccharide (eps)-degrading activity. a putative promoter and a riboso ...200018944430
infection behavior of venturia nashicola, the cause of scab on asian pears.abstract the infection of japanese pear by venturia nashicola, the cause of scab on asian pears (japanese pear, pyrus pylifolia var. culta; chinese pear, p. ussuriensis), was examined using light and electron microscopy to determine the mechanism of resistance in pears. early stages of infection were similar on the susceptible cv. kosui, the resistant cv. kinchaku, and the nonhost european pear (p. communis) cv. flemish beauty. v. nashicola penetrated only the cuticle layer on pear leaves and fo ...200018944422
using pseudomonas spp. for integrated biological control.abstract pseudomonas spp. have been studied for decades as model organisms for biological control of plant disease. currently, there are three commercial formulations of pseudomonads registered with the u.s. environmental protection agency for plant disease suppression, bio-save 10 lp, bio-save 11 lp, and blightban a506. bio-save 10 lp and bio-save 11 lp, products of jet harvest solutions, longwood, fl, contain pseudomonas syringae strains esc-10 and esc-11, respectively. these products are appl ...200718944382
duplex real-time polymerase chain reaction reveals competition between erwinia amylovora and e. pyrifoliae on pear blossoms.erwinia amylovora and e. pyrifoliae are the causative agents of fire blight and asian pear blight, respectively. the pathogens are closely related, with overlapping host ranges. data are unavailable on the current distribution of e. pyrifoliae and on the interaction between the two species when they are present together on the same host. in this study, a duplex real-time polymerase chain reaction (pcr) protocol was developed to monitor the population dynamics of e. amylovora and e. pyrifoliae on ...200818944291
frequency and seasonal distribution of pear psylla infected with the pear decline phytoplasma in california pear orchards.abstract pear decline (pd) is an important disease of pyrus communis fruiting cultivars in europe, asia, and the americas. pd is caused by a phloem-limited phytoplasma that, in california, is transmitted from diseased to healthy trees by pear psylla, cacopsylla pyricola. the percentage of phytoplasma-infected pear psylla has never been assessed in the united states in field-collected insects. pear psylla were collected monthly from pd-infected trees from three orchards in northern california. in ...200218944248
occurrence and identification of phytophthora spp. pathogenic to pear fruit in irrigation water in the wenatchee river valley of washington state.abstract seven hundred forty-nine isolates of phytophthora spp. were obtained from irrigation canals in eastern washington state during the 1992 to 1995 and 1999 growing seasons. isolates were retrieved using pear baiting techniques. all isolates were pathogenic to pear and were present in irrigation water beginning early in fruit development. over the course of the 5 year study, 10 and 5% of isolates were identified as p. cactorum and p. citricola, respectively, using morphological criteria. th ...200218944247
antibiosis contributes to biological control of fire blight by pantoea agglomerans strain eh252 in orchards.abstract fire blight, caused by erwinia amylovora, is the most serious bacterial disease of pear and apple trees. biological control with strains of pantoea agglomerans (syn. erwinia herbicola) may provide an effective disease management strategy for fire blight. most strains of p. agglomerans evaluated for suppression of fire blight produce compounds that inhibit the growth of e. amylovora in culture. the role of these inhibitory compounds in fire blight suppression in orchard environments has ...200218944246
a statistical comparison of the blossom blight forecasts of maryblyt and cougarblight with receiver operating characteristic curve analysis.abstract blossom blight forecasting is an important aspect of fire blight, caused by erwinia amylovora, management for both apple and pear. a comparison of the forecast accuracy of two common fire blight forecasters, maryblyt and cougarblight, was performed with receiver operating characteristic (roc) curve analysis and 243 data sets. the rain threshold of cougarblight was analyzed as a separate model termed cougarblight and rain. data were used as a whole and then grouped into geographic region ...200718944181
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