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a multiple fission-like mode of asexual reproduction in blastocystis hominis.a non-axenic and an axenic isolate of blastocystis hominis have been induced to form cysts in vitro using an encystation medium. the morphology of the parasite at different time points was observed by scanning electron microscopy. in day-2 cultures the cysts were spherical and had a non-uniform, coarse outer surface around the body. a deep, pore-like opening was seen in some of the parasites. most of the cysts from day-4 and day-6 cultures ruptured, revealing small, uniformly sized spherical bod ...19947809004
a concentration technique for obtaining viable cysts of blastocystis hominis from faeces. 19947799511
tubulovesicular elements in blastocystis hominis from the caecum of experimentally-infected rats.by transmission electron microscopy (tem), tubulovesicular elements were seen in blastocystis hominis obtained from the caecum of experimentally-infected rats. these appeared to arise from the peripheral cytoplasm and were rounded, oval or elongate in sections. it is suggested that these elements form a network for transfer of nutrients to the periphery during the process of encystation.19957797363
blastocystis hominis infection, a cause of human diarrhea.blastocystis hominis has long been described as a non pathogenic protozoan parasite until recently when claims have been made that it can result in pathogenic conditions. of the 729 stool samples (614 from survey and 115 from pediatric wards) examined, 18.1% of them were found to be positive for one or more intestinal protozoan cyst. the commonest was giardia intestinalis (8.4%) followed by entamoeba coli (7.1%) and entamoeba histolytica (5.1%) in the normal children without symptoms of diarrhea ...19947777913
[comparison of intestinal parasite infestation indexes among hiv positive and negative populations].this study was carried out on two groups of patients treated at the josé de san martín clinical hospital. one group was composed of 82 hiv-seropositive patients with no signs of diarrhea, and another one of 300 patients, not suspected of hiv infection, was considered as "control population". stool samples were collected from each patient and examined for intestinal parasites. these determinations were carried out at the department of clinical biochemistry, in the same hospital. three specimens w ...19947715428
biochemical characterisation of human isolates of blastocystis hominis.sds-page and iso-enzyme analysis of 11 human isolates of blastocystis hominis revealed at least two variants with different polypeptide patterns and two zymodemes, respectively. this is the first iso-enzyme and the second protein analysis to indicate strain differences in b. hominis.19957707340
practice guidelines for ordering stool ova and parasite testing in a pediatric population. the alberta children's hospital.a comprehensive utilization review was done of all stool ova parasite examinations performed at a pediatric hospital during a 3-year period from june 1, 1989 to july 1, 1992. a total of 2,652 stool specimens were surveyed from 1,532 children. forty-one percent (1,081) of the workload was from inpatients, 37% (976) was from emergency room (er) visits/other outpatients, and 22% (233) was from patients attending the gastroenterology (gi) clinic. the prevalence of enteric parasites in hospitalized c ...19957677114
a comparison of direct microscopy with culture for the diagnosis of blastocystis hominis. 19947667737
morphology of the cyst of blastocystis hominis. 19947667736
[prevalence of infection by intestinal helminths and protozoa in school children from a coastal locality in the province of valdivia, chile].during july-august 1989, 219 coprological samples from primary school children from niebla and los molinos localities (39 degrees 52's, 73 degrees 26'w) in the coast of province of valdivia, chile, were analysed. prevalence % (in parentheses) of infection by intestinal protozoa and helminths were the followings: entamoeba histolytica: (18.0), entamoeba coli (34.0), endolimax nana (34.4), iodamoeba buetschlii (7.4), blastocystis hominis (64.3), giardia intestinalis (27.9) chilomastix mesnili (0.8 ...19947654292
bacteria, viruses, yeasts and protozoans associated with diarrheal disease in singapore.labile toxin producing enterotoxic e. coli (etec) were the commonest pathogen isolated from diarrheal stools of hospitalized children (21%) and adults (26%) in singapore. salmonellas ranked a close second in children (19%). other bacterial pathogens were isolated from less than 5% of subjects. blastocystis hominis was detected in 4.3% of diarrheal stools when a simple sedimentation technique was used. cryptosporidium was not detected at all. an analysis of yeast counts in smears of diarrheal and ...19957603751
is blastocystis hominis a cause of diarrhea in travelers? a prospective controlled study in nepal.although the pathogenicity of blastocystis hominis has been extensively debated in the medical literature, controlled studies of the association between b. hominis and diarrhea are lacking. we conducted a case-control study among expatriates and tourists in kathmandu, nepal, in which we compared the prevalence of the organism among patients with diarrhea to that among a control group without diarrhea. b. hominis was detected in 56 (30%) of 189 patients with diarrhea, compared with 40 (36%) of 11 ...19957578767
is there any reason to continue treating blastocystis infections? 19957578717
blastocystis hominis and traveler's diarrhea. 19957578716
prevalence of intestinal parasitic infection in five farms in holambra, são paulo, brazil.a parasitological survey was carried out on 222 inhabitants of five farms in holambra, located 30 km north of campinas, são paulo, brazil, on october 1992. approximately 70% of the inhabitants were found to be infected with at least one species of intestinal parasite. the positive rates of 6 helminths and 7 protozoan species detected are as follows: 5.4% ascaris lumbricoides; 8.6% trichuris trichiura; 19.8% necator americanus; 10.4% strongyloides stercoralis; 1.4% enterobius vermicularis; 0.9% h ...19957569635
ultrastructure of blastocystis hominis cysts.blastocystis hominis cysts were concentrated from human faeces by repeated washing in distilled water and centrifugation on ficoll-paque. this procedure gave a concentrated suspension of cysts without yielding any non-cystic forms. the cysts were examined by both transmission (tem) and scanning electron microscopy (sem). cysts were surrounded by a fibrillar layer that appeared porous in sem. many naked cysts without the fibrillar layer were also visible in sem. they were variable in shape but mo ...19957567903
[morphology of blastocystis hominis in feces and evaluation of parasitological methods].a study of the morphology of blastocystis hominis in stool in ninety-four cases humans is described as central body, ameba and granular form were found as previously described. in addition a "globulose" form as a variation of granular form is first described. the central body form (96.8%) was the most abundant form. three parasitological methods as direct microscopical examination sample with saline solution 0.85%, lugol, sudan iii, stained with quensel, iron hematoxylin and culture are evaluate ...19947557277
light and electron microscopic localization of lipids in blastocystis hominis.localization of the lipid of blastocystis hominis was histochemically demonstrated. b. hominis was fixed with glutaraldehyde, treated with detergents, and stained with sudan black b or nile blue. the central vacuole of b. hominis stained with sudan black b showed many black droplets with great variation in staining intensity. in nile blue staining, the central vacuole showed many pinkish granules or homogeneous blue reactions. these results indicated the presence of neutral lipids and/or acidic ...19957544390
histochemical detection of carbohydrates of blastocystis hominis.the carbohydrates of blastocystis hominis were detected by histochemical techniques using light and electron microscopy. b. hominis, fixed with various fixatives, followed by treatment with detergents, were stained with periodic acid-schiff (pas) or alcian blue (ab). intense pas reactions were observed in cells fixed with glutaraldehyde or 1/2 karnovsky fixative. the cells fixed with other fixatives showed weak or no reactions with pas staining. similar results were seen in the case of ab stain. ...19957537145
differentiation of the various stages of blastocystis hominis by acridine orange staining.acridine orange staining differentiates the cystic and the central body forms of blastocystis hominis and offers a very convenient and easy method to observe the internal structure of the parasite. acridine orange stains the nuclei and the central body of the rounded vacuolar forms of the parasite bright and dull green, respectively. the colour changes to yellow and then to flaming red-orange when the rounded central body forms of the parasite become cystic.19947521862
morphological diversity of blastocystis hominis in sodium acetate-acetic acid-formalin-preserved stool samples stained with iron hematoxylin.the objective of this investigation was to study the morphological characteristics of blastocystis hominis in sodium acetate-acetic acid-formalin-preserved stool samples. routinely processed samples were examined for morphological detail, including size, shape, nuclear detail, and central body characteristics. morphological findings revealing the importance of recognizing b. hominis in the diagnostic laboratory are described.19947510311
serum antibody detected by fluorescent antibody test in patients with symptomatic blastocystis hominis infection. 19957501905
elucidation of the life cycle of the intestinal protozoan blastocystis hominis.this paper elucidates the status of the different morphological forms of blastocystis and reports the existence of thin- and thick-walled cysts in b. hominis on the basis of current experimental evidence. it is suggested that the thin-walled cysts are autoinfectious, leading to multiplication of the organism in the intestinal tract. the thick-walled cysts are responsible for external transmission via the faecal-oral route. a life cycle for b. hominis is postulated on the basis of these findings.19957501648
[frequency and significance of parasitic infections in refugees from vietnam (author's transl)].in the course of the medical care which is provided for the refugees from southeast asia 100 persons were tested for intestinal parasites (helminths and protozoa). in addition serum samples were obtained from 72 of these and tested in several tests (cft, fat, iha and sft) using antigens of entamoeba histolytica, leishmania donovani, toxoplasma gondii, schistosoma spp., fasciola hepatica, echinococcus spp., trichinella spiralis and litomosoides carinii. in 49 persons infections with one, two or ...19807395245
blastocystis hominis: improved axenic cultivation. 19817199211
generation time and growth rate of the human intestinal parasite blastocystis hominis.blastocystis hominis, an anaerobic intestinal protozoan parasite of man, has a generation time (gt) in axenic culture of 8.5-19.4 h. depending on the strain tested. average gt of the eight strains was 11.7 h. zero growth time cell counts of 5.0 x 10(5)/ml to 2.0 x 10(6)/ml rose in 3-5 days to 1 x 19(7) cells/ml. the gt was determined for the 24-hr period during which the most rapid growth occurred; about 2% of the b. hominis cells were in division during this time. division under the culture con ...19817198685
blastocystis hominis in a pig-tailed macaque: a potential enteric pathogen for nonhuman primates.large numbers of blastocystis hominis were found in fecal samples from a pig-tailed macaque (macaca nemestrina) with chronic diarrhea that was refractory to conventional therapy. the animal's condition improved and fecal samples showed minimal numbers of blastocystis hominis following therapy with an antiprotozoal agent. this organism, recently reclassified as a protozoa, appeared to be causally related to the diarrhea. the cytologic and ultrastructural features of the monkey blastocystis were c ...19807191935
[what should one believe about blastocystis hominis?]. 19816819414
ultrastructural aspects of blastocystis hominis. 19836687040
in vitro response of blastocystis hominis to antiprotozoal drugs.ten antiprotozoal drugs were tested in vitro against four axenic strains of the intestinal parasite blastocystis hominis. inhibitory drugs in order of effectiveness were emetine, metronidazole, furazolidone, trimethoprim sulfamethoxazole, 5-chloro-8-hydroxy-7-iodo-quinoline (entero-vioform), and pentamidine. moderately inhibitory were two quinolines other than iodochlorhydroxquin. these were chloroquine and 5,7-diiodo-8-hydroxy-quinoline (floraquin). diloxanide furoate was not inhibitory. paromo ...19836631776
[blastocystis hominis: morphological study using optical and electron microscopy].blastocystis hominis is a member of the phylum protozoa as described by zierdt et al. despite the complexity of its yet not well defined biological cycle, blastocystis was found to possess all the structures characterizing protozoans. the physiochemical and biological growth conditions are those of many protozoans. the high degree of cell differentiation is probably due to the existence of two reproduction modes.19836368020
clinical relevance of blastocystis hominis. 19846144939
blastocystis hominis: a neglected cause of diarrhoea? 19846143904
protozoan characteristics of blastocystis hominis. 19676058384
[occurrence of blastocystis hominis in turkey and morphological characteristics of this organism (apropos of a case)]. 19695399002
studies of blastocystis hominis. 19734690644
[blastocystis hominis brumpt, 1912, (phycomycetes) in the female genital tract]. 19724537319
blastocystis hominis: axenic cultivation. 19744369819
ultrastructure of blastocystis hominis. 19734360331
a comparison of the outer layers of blastocystis hominis with the cyst walls of certain common intestinal protozoa. 19714329022
blastocystis hominis in kathmandu, nepal. 19854058539
blastocystis hominis enteritis. 19854047965
ketoconazole and resistant blastocystis hominis infection. 19854040725
blastocystis hominis infection presenting as recurrent diarrhea. 19854038860
ultrastructural aspects of blastocystis hominis strain resistant to antiprotozoal drugs. 19854038050
afebrile diarrhea and blastocystis hominis. 19854014917
cytochrome-free mitochondria of an anaerobic protozoan--blastocystis hominis.isolated mitochondria of the anaerobic protozoan blastocystis hominis were subjected to spectral analysis, color, catalase, and peroxidase tests and found to be completely negative for cytochrome enzymes, catalase, and peroxide. based on the absence of cytochrome enzymes, the possible evolution of b. hominis mitochondria from anaerobic bacteria is postulated.19863959009
etiology and epidemiology of diarrheal diseases in the united states.accurate data on the frequency of acute diarrheal illness and the distribution of pathogens are not available for several reasons, including the facts that only a small fraction of cases come to the attention of physicians and that available diagnostic tests establish an etiology in only about half of these. in a survey of three groups of patients in a community (upper-middle class and lower class outpatients and hospitalized infants), a possible cause was found in fewer than 20 percent of outpa ...19853893118
parasitological observations of chronic diarrhoea in suspected aids adult patients in kinshasa (zaire).the parasitological profile of chronic diarrhoea in 46 zairian adults suspected of aids demonstrated that the frequency of protozoa was five times higher than that of helminths; 86% of the protozoa were sporozoa: isospora belli was the most frequent (19%), followed by cryptosporidium isolated for the first time in zaire (8%) and blastocystis hominis (2%). 37 of the 46 patients were immunodeficient.19863787692
[a case from practice (66). patient: e.k., born oct. 10, 1948, housewife (blastocystis hominis infection)]. 19863787022
association of blastocystis hominis with signs and symptoms of human disease.purged stools from 389 patients were evaluated microscopically for the presence of blastocystis hominis. a total of five or more b. hominis cells per 40x field were observed in 43 patients (11%), and b. hominis was the only intestinal parasite present in 23 (6%) of these patients. of the 23 patients, 19 had symptoms which included abdominal discomfort (15 patients), anorexia (10 patients), diarrhea (9 patients), and flatus (9 patients). the remaining four patients were asymptomatic. the proporti ...19863771743
blastocystis hominis: pathogen or fellow traveler?blastocystis hominis, an intestinal organism of uncertain taxonomic position, has long been considered nonpathogenic. some recent studies suggest, however, that it may be associated with diarrhea and may respond to treatment with iodoquinol or metronidazole. to investigate this possibility, we identified 148 persons whose stools contained this organism. of this number, 32 had at least 6 stool examinations performed. twenty-seven of the 32 persons were later found to have greater than or equal to ...19863766850
blastocystis hominis: evidence for human pathogenicity and effectiveness of metronidazole therapy.1. clinical symptoms and oral treatment with metronidazole were studied in 103 patients with pure infections by blastocystis hominis. 2. the results showed that excessive flatulence is the chief gastrointestinal symptom associated occasionally with diarrhoea and abdominal cramps. all the patients showed good responses with treatment of metronidazole and 74 patients whose stools were reexamined 1-2 months after the treatment demonstrated no signs of infections. 3. it is concluded that b. hominis ...19873665198
light-microscopical appearance and ultrastructure of blastocystis hominis, an intestinal parasite of man.a study on blastocystis hominis was undertaken for the purpose of clarifying the morphology of the organism using the following techniques; giemsa, heidenhain iron hematoxylin, and dapi stains, phase-contrast microscopy, and transmission- and scanning-electron microscopy. microscopic examinations of the lumen fluids aspirated at the endoscopical examination revealed the habitation of b. hominis in the lower ileum and cecum of a patient. when examined light-microscopically, organisms from stool m ...19873660977
[prevalence of blastocystis hominis in refugees]. 19873622300
diarrhea due to blastocystis hominis: an old organism revisited.we have reported a case of diarrhea caused by blastocystis hominis, an intestinal protozoan parasite of man. the organism is present in small numbers in up to one fifth of stool samples in hospitalized patients, but is associated with diarrhea in only heavily infested patients. typical symptoms include diarrhea, crampy abdominal pain, nausea, vomiting, low-grade fever, gas, malaise, and chills. fecal leukocytes are occasionally seen. the pathophysiologic mechanism of the diarrhea is not clear. n ...19873603119
cryptosporidiosis in ogun state, south-west nigeria.during the period of one month in the rainy season in ogun state in south-west nigeria 479 stool samples (420 of which were diarrhoea stool samples) were examined for cryptosporidiosis. oocysts were detected in 2.3% of all stools, in 2.6% of diarrhoeal stools and in 5.3% out of 150 children with diarrhoea. cryptosporidium was the sole pathogen detected in six of 11 cases. in addition to cryptosporidia also entamoeba histolytica, blastocystis hominis, giardia lamblia, ascaris lumbricoides, and tr ...19873602839
epidemiology and clinical features associated with blastocystis hominis infection.a retrospective chart review was performed on 100 patients infected with blastocystis hominis (bh) and 50 randomly selected age and sex matched controls to examine the clinical and epidemiologic features associated with this organism. the finding of greater than 5 bh per oil immersion field (1,000 x) was significantly associated with acute presentation of symptoms but was not predictive of the presence of gastrointestinal symptoms. of patients infected with bh, 57.5% had recently travelled to th ...19873449317
etiology of diarrhea among travelers and foreign residents in nepal.a bacterial pathogen was isolated from 47% of 328 expatriate patients with diarrhea seen at two medical clinics in nepal in 1986. enterotoxigenic escherichia coli (24%), shigella (14%), and campylobacter species (9%) were isolated most frequently. enteroinvasive and adherence factor-positive e coli were isolated from 2% and 1% of patients, respectively. giardia lamblia was detected in 12% of patients, rotavirus in 8%, and cryptosporidium and entamoeba histolytica each in 5%. blastocystis hominis ...19883404637
blastocystis hominis, brumpt 1912: are there different stages or species? 19883387411
presumptive evidence for blastocystis hominis as a cause of colitis.a patient with persistent diarrhea was found to have biopsy-proved colitis with large numbers of the protozoan blastocystis hominis present in stool. extensive evaluation failed to reveal any other potential etiologic agent of acute colitis. following treatment with a course of metronidazole, the patient became asymptomatic, b hominis was no longer present in stool, and results of a repeated biopsy were normal. these observations are consistent with the role of b hominis as a gastrointestinal pa ...19883365077
association of blastocystis hominis with human disease. 19883356799
intestinal parasites in children with diarrhea in el salvador.in a study of parasitological prevalence in el salvador, stool specimens of 210 children with diarrhea were examined for intestinal parasites. in 104 cases (49%), intestinal helminths and protozoa were found. 53.4% of the specimens showed single infestation, 31.7% showed double. 3% triple and 1% quadruple infestation. of the helminths, 31% were trichuris trichiura, 18% ascaris lumbricoides and 5% hymenolepis nana. of the pathogenic protozoa, 7% were identified as giardia intestinalis, 6% entamoe ...19883262555
[blastocystis hominis: a new pathogenic agent?]. 19883255133
[enteritis caused by blastocystis hominis]. 19883236933
freeze-fracture study of blastocystis hominis.the ultrastructure of blastocystis hominis was investigated by the freeze-fracture method. freeze-fracture replicas of the membranes of b. hominis and its organelles were studied with special regard to the density and distribution of the intramembranous particles (imp's). on all membrane replicas, the concentration of imp's on the protoplasmic face (p face) invariably was greater than on the exoplasmic face (e face). on the p face, imp's were heterogeneously distributed in dense aggregates, alte ...19883199336
enzyme immunoassay for detection of giardia lamblia cyst antigens in formalin-fixed and unfixed human stool.an antigen-capture enzyme-linked immunosorbent assay employing rabbit and mouse antisera to giardia lamblia cyst antigens was developed for the diagnosis of giardia infection through detection of g. lamblia-specific stool antigens in cell-free aqueous eluates of human stool. this is the first report of the use of anti-cyst antibodies in an enzyme immunoassay for g. lamblia. the assay gave a positive result with 54 of 59 stools from patients with symptomatic, clinically diagnosed giardiasis, givi ...19883183015
a survey of gastrointestinal parasites in ogun state, southwest nigeria.to study the epidemiology of intestinal parasites in ogun state, nigeria, 479 stool specimens were examined at a hospital in abeokuta during the rainy season in june 1986. intestinal helminths and/or protozoa were present in 297 samples (62%). 41.1% of the specimens showed a single infection, 34% showed double, 20% triple and 5% quadruple infection. the most commonly found worm was ascaris lumbricoides (in 40% of specimens), followed by trichuris trichiura (23.2%), hookworms (19.2%), strongyloid ...19883178337
blastocystis hominis: clinical and therapeutic aspects. 19883176148
morphology and frequency distribution of protozoan blastocystis hominis. 19883148761
light microscopical study of blastocystis spp. in monkeys and fowls.an investigation of a blastocystis species obtained from several species of monkeys and fowls was conducted to clarify the morphology of the organism, using light microscopical techniques including giemsa, heidenhain iron hematoxylin, trichrome stains, iodine mount and phase-contrast microscopy. a comparison was made with blastocystis hominis from humans. blastocystis spp. were found in 15 out of 26 monkeys and in all of 12 fowls (10 chickens and 2 ostriches) examined. the behaviour of the paras ...19873122203
[blastocystis hominis, a largely unknown cause of diarrhea]. 19873115746
parasitic diarrhea.this article reviews the biology, epidemiology, and clinical management of amebiasis, giardiasis, and cryptosporidiosis as well as the less common intestinal protozoa, dientamoeba fragilis, isospora belli, balantidium coli, and blastocystis hominis.19883074123
[blastocystis hominis--intestinal parasite or commensal?].blastocystis hominis has long been classified among blastomycetes. it was considered to be an apathogenous fungal organism which was very frequently detected in routine stool examinations. following the first studies with an electronic microscope in 1967, zierdt et al. (34) classified blastocystis hominis among the protozoa. since then, there have been increasingly frequent reports that blastocystis hominis can be an important cause of intestinal diseases especially diarrhea. for a definite taxo ...19883059694
blastocystis hominis: an organism in search of a disease.blastocystis hominis is a protozoan organism frequently found in the human intestinal tract. eleven consecutive patients with symptoms of enteritis and having b. hominis as the sole enteropathogen were studied in an attempt to define the association of blastocystosis with clinical disease. b. hominis could not be implicated as the etiologic agent of enteritis in any of these patients. all eleven had alternative (and usually noninfectious) explanations for their intestinal symptoms. there was no ...19883055191
blastocystis hominis infection during aids. 19882904075
lack of serum immune response to blastocystis hominis. 19872889924
blastocystis hominis and human disease. 19872887585
polymicrobial aetiology of travellers' diarrhoea.of 35 us peace corps volunteers in thailand, 20 (57%) had a total of 30 episodes of diarrhoea during their first 6 weeks in the country. enteric pathogens were associated with 90% of the episodes. a single pathogen was identified in 17 (57%) episodes, 2-4 pathogens were identified in 10 (33%) episodes, and there were 15 symptomless infections. enterotoxigenic escherichia coli (etec) was identified in 37% of these episodes, and various salmonella serotypes were isolated in 33%. infections with 9 ...19852857430
biochemical and ultrastructural study of blastocystis hominis.this study was prompted by the paradox of strong presence of mitochondria in an anaerobic protozoan, recently reclassified from the yeasts. stemming from publication in 1911 to 1912, blastocystis hominis has been generally accepted as a harmless intestinal yeast of humans, with short standardized textbook (parasitology) descriptions, even to the present day. reports since 1967 have changed the classification of b. hominis from yeast to protozoan (sarcodina), and this has been followed by interes ...19882838509
clinical significance of blastocystis hominis.a total of 19,252 stool specimens from 12,136 patients were examined by direct microscopy and the ethyl acetate-formalin concentration method during the last 2 years. all liquid specimens and those in which parasite identification was difficult or equivocal were also examined in trichrome-stained preparations. a total of 3,070 intestinal parasites were seen in 2,889 patients. blastocystis hominis was found in fecal material from 647 patients (17.5%). a total of 132 cases (25.6%) were observed to ...19892808664
blastocystis hominis as a human pathogen. 19892772470
blastocystis hominis infection in humans. 19892749107
terminal ileitis associated with blastocystis hominis infection.we report on the previously unobserved clinical presentation of terminal ileitis secondary to blastocystis hominis in a 37-yr-old white male. when the patient was treated with metronidazole, the symptoms improved and the radiographic abnormalities resolved. we believe that this is the first well-documented instance of terminal ileitis secondary to b. hominis.19892741890
blastocystis hominis gastroenteritis in a hemophiliac with acquired immune deficiency syndrome.blastocystis hominis is an enteric protozoan that has occasionally been associated with gastrointestinal illness in man but is not considered an opportunistic pathogen. we describe a 16-year-old hemophiliac with acquired immune deficiency-related complex in whom upper gastrointestinal symptoms in conjunction with high densities of b. hominis in duodenal secretions and stool developed. furazolidone treatment was associated with eradication of the organism and improvement in clinical symptoms. one ...19892732857
[blastocystis hominis, a parasitic cause of diarrhea].the frequency of detection of blastocystis hominis in the stools of patients with gastroenteritis is reported. over a twelve-month period b. hominis was identified in the stools of 69 (4.72%) out of 1460 patients. of these 69 positive samples, 45 (65%) contained b. hominis alone and in 24 (35%) it was present together with other parasites such as lamblia intestinalis, entamoeba histolytica, entamoeba coli, endolimax nana, iodamoeba buetschlii, ancylostoma duodenale, ascaris lumbricoides, trichur ...19892717900
ultrastructural variation of blastocystis hominis stocks in culture.an ultrastructural study of 10 different blastocystis hominis stocks was undertaken. three distinct morphological forms, vacuolar, granular and amoeboid, were distinguished. numerous variations in the organelles and general cell structure were observed between stocks. b. hominis displayed considerable size variation in the vacuolar forms, ranging from 4 to 63 micron. thickness and density of the surface coat varied between different stocks. beneath the surface coat the bilaminar cell membrane di ...19892707962
blastocystis hominis: phylogenetic affinities determined by rrna sequence comparison.in 1912 blastocystis hominis was identified as a new species and classified as a yeast (brumpt 1912). in the early 1920s several groups confirmed its classification as a yeast, specifically a member of the genus schizosaccharomyces (discussed by zierdt et al. 1967). apart from an occasional case report, the classification of b. hominis and its role as a harmless intestinal yeast was not questioned for another 50 years. then, zierdt (1967) suggested that it should be classified in the phylum prot ...19892649390
chromosomes of blastocystis hominis.three stocks of blastocystis hominis were adapted to monophasic culture in minimal essential medium (mem) and the chromosomes of these stocks separated by field inversion gel electrophoresis (fige). ten-twelve chromosomes were distinguished in the electrophoretic karyotype of these three stocks over the range 200 kilobase pairs to greater than 1 megabase pairs. the karyotype of each stock was different. three dna probes, b10, b30 and b31, derived from the netsky stock isolated in america were us ...19892635163
[observations on 4 atypical cases of blastocystosis].the paper reports on four atypical cases of blastocystosis with prevalence of digestive and cutaneous disorders and of the allergic and general phenomena. the diagnosis could be established only after revealing blastocystis hominis in the fecal parasitologic examination. the disappearance, in all the four cases, of varied clinical, delayed signs after treatment with metronidazol and stamycine (in two or even four repeated treatment schedules at 10 days' interval) is the proof that bl. hominis mi ...19892617001
blastocystis hominis--a potential intestinal pathogen.the parasite blastocystis hominis has been found in 10% to 18% of stool specimens submitted to microbiology laboratories. controversy exists as to whether this organism can cause illness in humans. we have reviewed the records of 65 symptomatic patients with b hominis in their stool. we conclude that b hominis is a potential pathogen that may or may not require drug therapy depending on the overall clinical circumstances, the severity of symptoms, and the presence of other pathogenic organisms.19892603418
questionable clinical significance of blastocystis hominis infection.in the period from january 1986 to july 1988, blastocystic hominis was found in moderate and numerous quantities in 103 (1.6%) of 6,262 stool specimens examined in our laboratory. there was no significant association of the detection of b. hominis with travel history or symptoms. indeed, 20 patients (36%) with moderate to heavy infections had no gastrointestinal symptoms, and three symptomatic patients did not show clinical improvement after elimination of the parasite. reexamination of stool sa ...19892596457
endocytosis in cultures of blastocystis hominis.a study of the function of the electron-dense pits in the vacuolar and granular forms of blastocystis hominis was undertaken. immuno-electron microscopy using anti-clathrin antibody and colloidal gold demonstrated clathrin to be associated with all forms of the pits and some cytoplasmic vesicles. cationized ferritin traced the pathway of endocytosis from the surface of the coated pits through internalization via electron-dense coated vesicles and uncoated vesicles and tubules in the cytoplasm. t ...19892592145
high incidence of false positives by a latex agglutination test for the diagnosis of clostridium difficile associated colitis in compromised patients.detection of clostridium difficile cytotoxin using cell culture assays for the diagnosis of antibiotic-associated colitis has been used for over a decade. because the methodology is time consuming and cumbersome, a recently introduced commercial latex agglutination (la) kit has attracted much attention. we compared the sensitivity and specificity of this method with the cytotoxic assay (cta) using diarrheal stools from 652 patients at a referral tertiary care center. specimens from 71 (10.9%) pa ...19892591165
family outbreak of blastocystis hominis associated gastroenteritis. 19892574330
blastocystis hominis chronic diarrhoea in aids patients. 19892563129
[incidence of infection by intestinal parasites among school children in santiago, chile, 1988-1989].in december 1988-february 1989 period, a survey on enteroparasitic infections in children (mean age 8.7 years; males 54.4% and females 45.6%) from five schools located in santiago, chile, was undertaken. to each of these individuals, three samples were obtained in order to perform in them the following examinations: co-proparasitological study, modified ziehl-neelsen staining for detecting cryptosporidium sp. and cellulose adhesive test for enterobius vermicularis. parasitic elements were found ...19892484851
blastocystis hominis: epidemiology and natural history.to study the demographic profile of blastocystis hominis carriers from hamilton, canada, the regional parasitology laboratory records for 1988 were reviewed, and a prospective study on carriers was conducted to clarify the natural history of the infection and ascertain the role of b. hominis as an intestinal pathogen. retrospective analysis revealed that 8% of stool samples harbored b. hominis. the median age of the carriers was 37 years; 55% were female. prospective analysis of 139 patients sho ...19902401797
blastocystis hominis. 19902353482
association of blastocystis hominis with human disease? 19902351728
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