Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| single-stranded-dna-dependent atpase from hela cells that stimulates dna polymerase alpha-primase activity: purification and characterization of the atpase. | a single-stranded dna-dependent atpase that cofractionates during the early stages of purification of a multiprotein dna polymerase alpha complex from hela cells has been purified to homogeneity. the atpase is part of a 16s multienzyme dna polymerase alpha complex that is fully active in sv40 dna replication in vitro. the atpase hydrolyzes atp to adp in a reaction that is completely dependent on the presence of dna. dna in single-stranded form is strongly preferred as a cofactor, and polydeoxynu ... | 1990 | 2148684 |
| random peptide libraries: a source of specific protein binding molecules. | libraries of random peptide sequences were constructed and screened to identify peptides that specifically bind to proteins. in one of these about 2 x 10(7) different 15-residue peptide sequences were expressed on the surface of the coliphage m13. each phage encoded a single random sequence and expressed it as a fusion complex with piii, a minor coat protein present at five molecules per phage. phage encoding nine different streptavidin-binding peptide sequences were isolated from this library. ... | 1990 | 2143033 |
| the function of a leader peptide in translocating charged amino acyl residues across a membrane. | insertion of bacteriophage coat proteins into the membrane of infected bacterial cells can be studied as a model system of protein translocation across membranes. the coat protein of the filamentous bacteriophage pf3--which infects pseudomonas aeruginosa--is 44 amino acids in length and has the same basic structure as the coat protein of bacteriophage m13, which infects escherichia coli. however, unlike the pf3 coat protein, the m13 coat protein is synthesized as a precursor (procoat) with a typ ... | 1990 | 2124001 |
| stable expression of meningococcal class 1 protein in an antigenically reactive form in outer membranes of escherichia coli. | the entire gene encoding the class 1 outer membrane protein of neisseria meningitidis is located on a 2.2kb fragment, obtained on digestion of chromosomal dna with xbal. this xbal fragment from strain mc50 (subtype p1-16), which had previously been cloned in bacteriophage m13, has been transferred to the plasmid vector pmtl20. the resulting plasmid (ppora100) was propagated in escherichia coli (jm109) and cell lysates were subjected to sds-page. western blotting with anti-class 1 protein antibod ... | 1990 | 2117694 |
| translational regulation of m13 gene ii protein by its cognate single-stranded dna binding protein. | to unravel the mechanism by which the single-stranded dna binding protein encoded by gene v of the filamentous phage m13 regulates the synthesis of its cognate dna replication protein encoded by gene ii, an in vivo test system has been developed. the system consists of two recombinant plasmids with compatible replication origins. one plasmid contains m13 gene v under the control of the inducible arab promoter of salmonella typhimurium. the other plasmid contains a fusion gene, whose expression i ... | 1990 | 2110060 |
| a critical arginine in the large subunit of ribulose bisphosphate carboxylase/oxygenase identified by site-directed mutagenesis. | rapid inactivation by phenylglyoxal of ribulose bisphosphate carboxylase/oxygenase (ribulose-p2 carboxylase) from the cyanobacterium anacystis nidulans suggests the presence of an essential arginine, the modification of which is reduced in the presence of the substrate ribulose bisphosphate. arginine 292 in the large subunit of ribulose-p2 carboxylase from a. nidulans was chosen for site-directed mutagenesis studies on the basis of the complete conservation of this residue in corresponding seque ... | 1990 | 2108139 |
| neutron and gamma-irradiation of bacteriophage m13 dna: use of standard neutron irradiation facility (snif). | we describe here the use of the van de graaff accelerator as a source of high energy neutrons for biological irradiation. single-stranded bacteriophage m13 dna was chosen as the system to determine the relative biological effectiveness of monoenergetic neutrons. a standard neutron irradiation facility (snif) was established using a 3 mv van de graaff accelerator. the 2d (d,n)3he nuclear reaction was used to produce neutron fluxes of 3 x 10(8) cm 2 sec-1 yielding dose rates as high as 50 gy h-1. ... | 1990 | 2098554 |
| molecular cloning of human satellite dna sequences and their use in detecting dna polymorphism. | a approximately 400 bp haeiii human genomic satellite dna band was cloned into puc18 to construct a partial library. a fragment of bacteriophage m13 containing a sequence homologous to the human minisatellite core was cloned in puc18 and was used as a probe to isolate a approximately 350 bp human satellite clone (ptrf5.6) from the partial library. other clones from this library showed a wide variation in terms of size and hybridization to the ptrf5.6 clone. human dna from different individuals w ... | 1990 | 2079331 |
| genetic requirements for frameshift reversion induced by bulky dna adducts in m13 dna. | in order to analyze the genetic requirements and mechanisms of frameshift mutagenesis by activated aflatoxin b1 (afb1), in vitro-modified phage m13 replicative form (rf) dna was transfected into appropriate escherichia coli cells and +1 or -1 frameshift revertants in the lacz(alpha) gene were isolated. this analysis shows that both +1 and -1 frameshift mutagenesis by afb1 is significantly reduced in a umuc- background. on the other hand, in the absence of reca, +1 frameshift mutagenesis is parti ... | 1991 | 2067532 |
| effects of mutations in glycosylation sites and disulphide bonds on processing, cd4-binding and fusion activity of human immunodeficiency virus envelope glycoproteins. | site-directed mutagenesis was used to study the biological significance of a disulphide bridge and two n-linked oligosaccharides in the cd4-binding region of the envelope glycoproteins of human immunodeficiency virus type 1. mutagenesis was performed in a phage m13 system at sites corresponding to the cysteine residue (amino acid 402) and the asparagine residues (390 and 447) of the env gene. the mutated env gene was inserted into a recombinant vaccinia virus under the control of the vaccinia vi ... | 1991 | 2045792 |
| an improved method for the detection of dcm methylation in dna molecules. | the intrinsic insensitivity of ecorii recognition sites in rf dnas of phage m13 and vector m13mp18 towards this restriction endonuclease can be overcome by adding site-specific oligodeoxyribonucleotide duplexes to the restriction sample. since dcm- dna but not dcm(+)-methylated dna becomes susceptible under these conditions, this procedure constitutes an improvement of the dcm methylation assay. | 1990 | 1979301 |
| isolation of the dna minisatellite probe mz 1.3 and its application to dna 'fingerprinting' analysis. | a minisatellite probe, mz 1.3, detecting hypervariable fragment patterns was isolated from a human genomic library. a repetitive sequence of 27 bp length was identified which is contained in the probe approx. 40 times. the mz 1.3 repeat shows variable homology of 53-73% to the repetitive sequence of the protein iii gene of the bacteriophage m13 genome. polymorphic restriction fragment patterns were found with mz 1.3 using the enzymes hinf i, bstn i, hae iii, mbo i, psti/pvu ii, and rsa i. an ave ... | 1990 | 1969380 |
| bst dna polymerase permits rapid sequence analysis from nanogram amounts of template. | a simple, rapid method is presented for the enzymatic sequence analysis of nanogram amounts of single-stranded or double-stranded dna. this approach employs the thermostable dna polymerase from bacillus sterothermophilus and exploits its ability to efficiently extend all of the template-primer complex, even at low substrate concentrations. the procedure requires few pipetting steps, no preannealing step and very short reaction time. this method can significantly reduce the cost associated with d ... | 1991 | 1954022 |
| viable transmembrane region mutants of bacteriophage m13 coat protein prepared by site-directed mutagenesis. | bacteriophage m13 coat protein - a 50-residue protein located at the e. coli host membrane during phage reproduction - is subjected to cytoplasmic, membrane-bound, and dna-interactive environments during the phage life cycle. in research to examine the specific features of primary/secondary structure in the effective transmembrane (tm) region of the protein (residues 21-39: yigyawamvvvivgatigi) which modulate its capacity to respond conformationally to the progressive influences of these varying ... | 1991 | 1953741 |
| formation of single and double strand breaks in dna ultraviolet irradiated at high intensity. | the induction of single-strand breaks (ssb) by two quantum processes in dna is well established. we now report that biphotonic processes result in double-strand breaks (dsb) as well. puc19 and bacteriophage m13 rf dna were irradiated using an excimer laser (248 nm) at intensities of 10(7), 10(9), 10(10) and 10(11) w/m2 and doses up to 30 kj/m2. the proportion of dna as supercoil, open circular, linear and short fragments was determined by gel electrophoresis. linear molecules were noted at fluen ... | 1991 | 1946694 |
| the escherichia coli terb sequence affects maintenance of a plasmid with the m13 phage replication origin. | replication initiated at the bacteriophage m13 origin can be affected by interaction of a properly oriented termination signal terb and the tus protein. the effect can be alleviated by overproduction of the m13 replication gene protein ii. | 1991 | 1938965 |
| the in situ aggregational and conformational state of the major coat protein of bacteriophage m13 in phospholipid bilayers mimicking the inner membrane of host escherichia coli. | the major coat protein of bacteriophage m13 has been reconstituted into phospholipids with a composition comparable to that found in the host (escherichia coli) inner membrane. reconstitution experiments have revealed conditions in which the alpha-oligomeric state is favored over the beta-polymeric state. discrimination between the two states of the membrane-bound coat protein (alpha-oligomeric and beta-polymeric states) has been achieved using high-performance size-exclusion chromatography and ... | 1991 | 1932035 |
| dna fingerprints of sheep using an m13 probe. | the bacteriophage m13 dna was used to detect hypervariable minisatellites in several families of booroola sheep as well as merino and suffolk sheep. digestion of sheep dna gave rise to three to eight fragments with different restriction enzymes demonstrating considerable polymorphism between the different breeds. the length of informative dna fragments varied in size from 6 to 20kb. the dna fingerprints generated were individual specific and allowed for differentiation between closely related an ... | 1991 | 1928833 |
| 2'-deoxy-6-thioguanosine 5'-triphosphate as a substrate for purified human dna polymerases and calf thymus terminal deoxynucleotidyltransferase in vitro. | 2'-deoxy-6-thioguanosine 5'-triphosphate (s6dgtp), a metabolite of the antileukemia agent 6-thioguanine, was evaluated as a substrate for purified human dna polymerases. using bacteriophage m13 single-strand dna as a template, s6dgtp substituted efficiently for dgtp and stimulated dna synthesis in reactions without dgtp, with dna polymerases alpha, delta, and gamma from the human leukemia cell line k562. the apparent km values for dgtp and s6dgtp were very similar, i.e., 1.2 microm each for poly ... | 1991 | 1921985 |
| differentiation of species and strains among filamentous fungi by dna fingerprinting. | we have analyzed 11 strains and clones, representing five species (penicillium janthinellum, p. citrioviridae, p. chrysogenum, aspergillus niger, trichoderma harzianum) and three genera of filamentous fungi, for the presence of hypervariable loci in their genomes by hybridization with simple repeat oligonucleotides and the dna of phage m13. the oligonucleotide probes (ct)8, (gtg)5 and (gaca)4, as well as m13 dna, are informative probes for fingerprinting in all genera and species tested. the pro ... | 1991 | 1907892 |
| regulation of expression of the genome of bacteriophage m13. gene v protein regulated translation of the mrnas encoded by genes i, iii, v and x. | with the aid of a binary plasmid in vivo testsystem it was demonstrated that the single-stranded dna binding protein encoded by gene v of bacteriophage m13 not only regulates the synthesis of its cognate dna replication proteins at the level of translation, but also of the assembly proteins and the coat proteins encoded by genes i and ii, respectively. furthermore, gene v protein functions as a translational autoregulator of its own synthesis. comparison of the mrna levels of genes i and x in th ... | 1991 | 1905158 |
| assembly of combinatorial antibody libraries on phage surfaces: the gene iii site. | a phagemid system was developed for the monovalent display of combinatorial antibody fab libraries on the surface of filamentous phage m13. fab fragments were fused to the carboxyl-terminal domain of the gene iii protein. phage displaying fab fragments on their surface, or phabs, were enriched by 10(3)- to 10(5)-fold on antigen-coated surfaces over nonspecific phage. the method may replace current antibody cloning techniques. | 1991 | 1896445 |
| characterization of wild-type and mutant m13 gene v proteins by means of 1h-nmr. | recording of good quality nmr spectra of the single-stranded dna binding protein gene v of the bacteriophage m13 is hindered by a specific protein aggregation effect. conditions are described for which nmr spectra of the protein can best be recorded. the aromatic part of the spectrum has been reinvestigated by means of two-dimensional total correlation spectroscopy. sequence-specific assignments were obtained for all of the aromatic amino acid residues with the help of a series of single-site mu ... | 1991 | 1879419 |
| the replication termination signal terb of the escherichia coli chromosome is a deletion hot spot. | hybrids composed of phage m13, plasmid pbr322 and the termination signal of escherichia coli chromosome replication terb were used to show that arrest of dna synthesis creates a very efficient deletion hot spot. up to 80% of deletions occurring in these hybrids had one deletion end-point at terb provided that (i) terb was oriented to arrest m13 and pbr322 leading strand synthesis; and (ii) the host cells contained the tus protein necessary for arresting dna synthesis at terb. the position of ter ... | 1991 | 1868840 |
| dynamic light scattering from weakly bending rods: estimation of the dynamic bending rigidity of the m13 virus. | a theory is presented for the dynamic structure factor [s(k,t]) of weakly bending rods. this treatment is based on a discrete bead model for the brownian dynamics in which all bead motions associated with bending are constrained to occur in a plane perpendicular to the end-to-end vector, thus prohibiting extension or contraction along that axis. preset hydrodynamic interactions are incorporated in a numerically exact manner. the predicted normalized dynamic structure factor s(k,t)/s(k,0) should ... | 1991 | 1868169 |
| plasmid co1ib contains an ssi signal close to the replication origin. | taking advantage of the plaque morphology method, we identified a single-strand initiation (ssi) signal in plasmid psm32, a mini-co1ib plasmid. this ssi signal was situated in the 350-nt haeiii segment of the 1.8-kb s7 fragment, and located nearly 400 nt downstream of the origin of dna replication. introduction of the ssi signal into a mutant of filamentous phage m13 lacking oric resulted in restoration of phage growth and rfi dna synthesis. interestingly, dna homology studies showed that the nu ... | 1991 | 1857752 |
| [analysis of genetic distances between populations using human dna "fingerprints" detected by a phage m13 dna probe]. | the frequencies of different electrophoretic bands in dna fingerprints detected by phage m13 dna probe in two populations of the kirov district were determined. the dna polymorphisms observed in these two populations were compared with each other and with those of the krasnodar populations, and pseudogenetic distances were calculated. the mean genetic distance between two kirov populations was 0.072, this between every kirov and krasnodar population being 0.21 and 0.22. | 1991 | 1838094 |
| dna fingerprinting of the human intestinal parasite giardia intestinalis with hypervariable minisatellite sequences. | individual isolates of the giardia duodenalis group of protozoan intestinal parasites were identified by dna fingerprinting with hypervariable minisatellite sequences. a morphologically identical parasite is found in some forty different animal species. although the species name intestinalis is reserved for the human isolates, electrophoretic karyotyping suggests that most duodenalis isolates fall into the same species grouping. distinction based upon morphology, restriction endonuclease cleavag ... | 1991 | 1831167 |
| [use of dna polymorphism detected by m13 phage dna in population studies]. | hypervariable "minisatellite" regions detected in human genome by wild-type phage m13 dna were found to have high polymorphism and somatic stability. analysis of individual specific patterns of hybridization of 44 human dnas from the kirov province is presented. molecular weight of fragments varied from 2 to 6 kb. mean frequency of a fragment in the population under study is p = 0.294 +/- 0.158. the mean number of fragments per individual is 11.6 +/- 1.8. comparison between the kirov population ... | 1991 | 1830281 |
| shuttle plasmid vectors for lactobacillus casei and escherichia coli with a minus origin. | recombinant plasmids which can be used as shuttle vectors between escherichia coli and the industrially used strains of lactobacillus casei were constructed. they have replication regions closely related to those of pub110 and are likely to replicate by a rolling-circle mechanism via a plus-strand-specific dna intermediate in l. casei. both orientations of pala from the staphylococcal plasmid pc194 and those of the intergenic region from coliphage m13 are identified as active minus origins in l. ... | 1991 | 1781687 |
| experience in shotgun sequencing a 134 kilobase pair dna molecule. | until now, large dna sequences have been obtained by cloning fragments of the target molecule into plasmid, cosmid or bacteriophage lambda vectors. the 134 kbp dna sequence of channel catfish virus was determined with relative ease by shotgun cloning of random fragments of genomic dna directly into a bacteriophage m13 vector, sequencing by dideoxynucleotide chain termination, and compilation of the data using staden's database handling programs. experience gained during this endeavour indicates ... | 1991 | 1768862 |
| sequence-specific 1h-nmr assignment and secondary structure of the tyr41----his mutant of the single-stranded dna binding protein, gene v protein, encoded by the filamentous bacteriophage m13. | sequence-specific 1h-nmr assignments are reported for the tyr41----his (y41h) mutant of the single-stranded dna binding protein, encoded by gene v of the filamentous bacteriophage m13 (gvp). the mutant protein was chosen for this purpose because it exhibits significantly improved solubility characteristics over wild-type gvp [folkers et al. (1991) eur. j. biochem. 200, 139-148]. the secondary structure elements present in the protein are deduced from a qualitative interpretation of the nuclear o ... | 1991 | 1761038 |
| assignment of the 1h nmr spectrum and secondary structure elucidation of the single-stranded dna binding protein encoded by the filamentous bacteriophage ike. | by means of 2d nmr techniques, all backbone resonances in the 1h nmr spectrum of the single-stranded dna binding protein encoded by gene v of the filamentous phage ike have been assigned sequence specifically (at ph 4.6, t = 298 k). in addition, a major part of the side chain resonances could be assigned as well. analysis of noesy data permitted the elucidation of the secondary structure of ike gene v protein. the major part of this secondary structure is present as an antiparallel beta-sheet, i ... | 1992 | 1734970 |
| gene v protein-mediated translational regulation of the synthesis of gene ii protein of the filamentous bacteriophage m13: a dispensable function of the filamentous-phage genome. | introduction of a deletion in the genome of wild-type m13 bacteriophage that eliminates translational repression of m13 gene ii by its cognate gene v protein had no effect on phage viability. furthermore, it was noted that gene v protein of phage ike, a distant relative of m13, does not function as a translational repressor of its cognate gene ii protein. the data strongly indicate that the gene v protein-mediated control of gene ii expression in bacteriophage m13 is an evolutionary relic of the ... | 1992 | 1729248 |
| design, construction and function of a multicopy display vector using fusions to the major coat protein of bacteriophage m13. | incorporation of numerous copies of a heterologous protein (bovine pancreatic trypsin inhibitor; bpti) fused to the mature major coat protein (gene viii product; viii) of bacteriophage m13 has been demonstrated. optimization of the promoter, signal peptide and host bacterial strain allowed for the construction of a working vector consisting of the m13 genome, into which was cloned a synthetic gene composed of a lac (or tac) promoter, and sequences encoding the bacterial alkaline phosphatase sign ... | 1991 | 1721885 |
| influence of neighboring base sequence on mutagenesis induced by in vitro misincorporation in the laci gene of escherichia coli. | genetic and electrophoretic assays of misincorporation were used to assess the effect of dna sequence on mutagenesis arising from in vitro dna synthesis within the laci gene of escherichia coli. the viral strand of a derivative of phage m13 containing the entire laci gene was annealed with a series of synthetic oligonucleotides complementary to the n-terminal region of the laci gene. each primer-template was incubated with e. coli dna polymerase i (klenow fragment) under conditions favoring misi ... | 1991 | 1720871 |
| genetic assay of misincorporation. | a system to characterize mutations arising from in vitro nucleotide misincorporation, which avoids the effects of in vivo mismatch repair on recovery of mutants, was constructed and evaluated. the laci gene of escherichia coli was inserted into phage m13 and the m13-laci recombinant was introduced into a strain of e. coli lacking a resident laci gene. in this system the function of the m13-bearing laci gene can be detected by plaque color. mutants in the 5'-region of the laci gene (encoding oper ... | 1991 | 1720870 |
| [rna and dna synthesis catalyzed by a dna-polymerase alpha complex with primase from silkworm cells on single-stranded dna]. | depending on the ionic environment the replicative complex of silkworm bombyx mori, containing dna polymerase alpha and primase, catalyzes on single-stranded dna of phage m13 a ntp-dependent synthesis or elongation of preformed primers. in the presence of ntps and dntps at conditions optimal for the ntp-dependent synthesis the replicative complex synthesizes on m13 dna oligoribonucleotides of 9-11 residues, which serve as primers for polymerization of dna. the length of rna-primers synthesized b ... | 1991 | 1716736 |
| deletions in the trna(lys) primer-binding site of human immunodeficiency virus type 1 identify essential regions for reverse transcription. | the initiation of human immunodeficiency virus type 1 (hiv-1) reverse transcription occurs by the extension of a trna primer bound near the 5' end of the genomic rna at a position termed the primer-binding site (pbs). the pbs is an 18-nucleotide region of the hiv-1 genome complementary to cellular trna(lys). to further investigate the sequence requirements for the pbs in reverse transcription, deletions in the pbs were created and subcloned into a plasmid containing the infectious hiv-1 proviral ... | 1991 | 1714513 |
| two regions of m13 phage genome hybridizing with human dna are similar to several keratin genes. | dna from two regions of the phage m13 genome hybridizes with dna restriction fragments from genomes of various species including man [15, 20]. as the pattern of hybridization is individual-specific, this phage m13 probe can be used for dna fingerprinting. we demonstrate here that the regions of many keratin genes coding for glycine-rich parts of c and n end domains are very similar to the phage m13 probe, and this similarity may be responsible for hybridization. | 1990 | 1710508 |
| hormone phage: an enrichment method for variant proteins with altered binding properties. | human growth hormone (hgh), a 191 residue protein containing two disulfide bonds, was fused to the carboxyl-terminal domain of the gene iii protein, a minor coat protein exposed at one end of the filamentous phage m13. the gene fusion was cloned into a plasmid containing origins of replication for escherichia coli and filamentous phage and was packaged into phagemid particles upon infection by an m13ko7 helper phage. transcription of the hgh-gene iii fusion was controlled so that usually no more ... | 1990 | 1708882 |
| role of dna polymerase 3'----5' exonuclease activity in the bypass of aminofluorene lesions in dna. | n-(deoxyguanosin-8-yl)-2-(acetylamino)fluorene (aaf-g) adducts in the dna of bacteriophage m13 can be converted to n-(deoxyguanosin-8-yl)-2-aminofluorene (af-g) adducts in situ by treatment with 1.0 m naoh for 45 min at room temperature. the conversion is accompanied by a dramatic increase in the transfection activity of the samples which is correlated with the measured deacetylation of the acetylaminofluorene adduct. the pair of substrates (aaf-g/af-g) with adducts at identical places in the dn ... | 1990 | 1702368 |
| synthesis of dna by human immunodeficiency virus reverse transcriptase is preferentially blocked at template oligo(deoxyadenosine) tracts. | the genome of human immunodeficiency virus (hiv) and especially the envelope gene are mutated with unusually high frequency during in vivo replication. recent studies indicate that hiv reverse transcriptase (rt) is unusually error prone and that the number of generated mutations is disproportionately high within repetitive base sequences. to study the ability of recombinant and wild-type hiv rt to traverse specific homo-oligomeric stretches, we used bacteriophage m13 dna templates that contain d ... | 1990 | 1698789 |
| hepatitis b surface antigen particles with all four subtypic determinants: point mutations of hepatitis b virus dna inducing phenotypic changes or double infection with viruses of different subtypes. | hepatitis b surface antigen (hbsag) particles carry the common determinant, a, as well as d or y and w or r subtype determinants, and are classified into the four major subtypes, i.e., adw, adr, ayw and ayr. rare sera contain hbsag particles with all four subtype determinants (adywr). target sequences (nucleotides 38-550) in the s gene of hepatitis b virus (hbv) dna in two such sera were amplified by the polymerase chain reaction. individual amplification products were cloned in an m13 phage vec ... | 1990 | 1694959 |
| using the polymerase chain reaction to maintain dna probe inventories in clinical and diagnostic laboratories. | clinical and diagnostic dna laboratories must maintain a large inventory of dna probes for use in hybridization studies. the preparation of plasmid dna and isolation of dna fragments for use as probes in both expensive and time consuming. we present here a rapid and relatively inexpensive method of producing large amounts of dna fragments from stocks, using the polymerase chain reaction (pcr). our experience over the past year using this technique has been very positive and we believe many labor ... | 1991 | 1673093 |
| [study of the mutagenic properties of oligonucleotides containing and internucleotide pyrophosphate bond using a mutation system based on phage m13]. | mutagenic properties of oligonucleotides with pyrophosphate internucleotide bond was studied. it was shown that the pyrophosphate bond in the oligo structure does not induce mutations but promotes a more efficient induction of marker deletions predetermined by the nucleotide sequence as compared to the native oligonucleotide. marker deletion induction proceeds according to the repair mechanism as homozygotes dominate in the mutant generation. | 1991 | 1667540 |
| preferential transposition of an is630-associated composite transposon to ta in the 5'-ctag-3' sequence. | a composite transposon, tn4731, associated with is630 has been shown to transpose preferentially to 5'-ta-3' sequences that are located at two sites in a rho-dependent transcription terminator in plasmid cole1 in escherichia coli (t. tenzen, s. matsutani, and e. ohtsubo, j. bacteriol. 172:3830-3836, 1990). here we demonstrated that tn4731 preferentially transposes to ta sequences at four sites in plasmid puc118 and its derivatives: the ta sequence (hot spot i) in the intergenic region of phage m ... | 1991 | 1655702 |
| a bacteriophage m13 based sandwich hybridization assay for the detection of hepatitis b virus in human blood. | a two step hybridization procedure was developed to detect the presence of hepatitis b virus in blood samples using bacteriophage m13 radiolabelled dna as probe. during the first step of hybridization, single-stranded bacteriophage m13 tg 130 dna, with 3.2 kb hbv dna cloned into it, was hybridized to target hbv dna immobilized on nitrocellulose membrane filter. in the second step of hybridization, m13 dna annealed to hbv target is detected with the help of double stranded form of m13 dna. the as ... | 1991 | 1652551 |
| the nucleotide sequence of 3c proteinase region of the coxsackievirus a24 variant: comparison of the isolates in taiwan in 1985-1988. | acute hemorrhagic conjunctivitis caused by coxsackievirus a24 variant (ca24v) first appeared in taiwan in october 1985, followed by two other sequential epidemics in 1986 and 1988. in order to know the evolutionary relationship of the ca24v strains isolated in taiwan, we first determined the nucleotide sequence of the 3c proteinase (3cpro) region of the prototype strain (eh24/70), isolated in singapore in 1970, by molecular cloning. the nucleotide sequence of the 3cpro region thus sequenced show ... | 1991 | 1647565 |
| three-dimensional structure of a cloning vector. x-ray diffraction studies of filamentous bacteriophage m13 at 7 a resolution. | filamentous bacteriophage m13 is a single-stranded dna phage about 65 a in diameter and 9300 a long. x-ray diffraction studies of magnetically oriented fibers of native, mercury and iodine-labeled phage particles have been used to determine the arrangement of the major coat protein, the gene 8 product, in the virion. the coat protein is made up of a single gently curving alpha-helix extending from approximately pro6 to near the carboxyl terminus. the axis of the alpha-helix is tilted about 20 de ... | 1992 | 1640460 |
| survival of phage m13 with uracils on one or both dna strands. | the survival of m13 dna was studied after partial replacement of thymine by uracil in the bacteriophage. uracils carry the same genetic information as the thymines. nevertheless in escherichia coli wild-type cells, uracils in dna are replaced by thymines by excision repair initiated by uracil-dna glycosylase (udg). thus inactivation of uracil-containing phage dna is solely due to repair initiated by udg. incorporation of uracils was achieved in one or in both strands, either randomly or site-spe ... | 1992 | 1620092 |
| identification of potential active-site residues in the escherichia coli leader peptidase. | leader peptidase of escherichia coli cleaves the leader sequence from the amino terminus of membrane and secreted proteins after these proteins insert across the membrane. despite considerable research, the mechanism of catalysis of leader peptidase remains unknown. this peptidase cannot be classified using protease inhibitors to the serine, cysteine, aspartic acid, or metallo- classes of proteases (zwizinski, c., date, t., and wickner, w. (1981) j. biol. chem. 256, 3593-3597). using site-direct ... | 1992 | 1618816 |
| fluorescence studies of the binding of bacteriophage m13 gene v mutant proteins to polynucleotides. | this investigation describes how the binding characteristics of the single-stranded dna-binding protein encoded by gene v of bacteriophage m13, are affected by single-site amino acid substitutions. the series of mutant proteins tested includes mutations in the purported monomer-monomer interaction region as well as mutations in the dna-binding domain at positions which are thought to be functionally involved in monomer-monomer interaction or single-stranded dna binding. the characteristics of th ... | 1992 | 1606950 |
| assignment of amide 1h and 15n nmr resonances in detergent-solubilized m13 coat protein: a model for the coat protein dimer. | the major coat protein of the filamentous coliphage m13 is a 50-residue integral membrane protein. detergent-solubilized m13 coat protein is a promising candidate for structure determination by nuclear magnetic resonance methods as the protein can be prepared in large quantities and the protein-containing micelle is reasonably small. under the conditions of our experiments, sds-bound coat protein exists as a dimer with an apparent molecular weight of 27,000. broad lines and poor resolution in th ... | 1992 | 1606152 |
| inserting foreign peptides into the major coat protein of bacteriophage m13. | foreign dna fragments were inserted into filamentous phage gene viii to create hybrid b-proteins with foreign sequences in the amino terminus. the hybrid proteins are incorporated into the virions which retain viability and infectivity. virions with hybrid b-proteins have the same contour length and the same number of b-protein molecules as virions with natural b-proteins. it was shown that for one of hybrid b-proteins the position of the processing site had changed. | 1992 | 1577174 |
| selection and characterization of randomly produced mutants of gene v protein of bacteriophage m13. | gene v protein of bacteriophage ff (m13, f1, fd) is a master regulator of phage dna replication and phage mrna translation. it exerts these two functions by binding to single-stranded viral dna or to specific sequences in the 5' ends of its target mrnas, respectively. to study the structure/function relationship of gene v protein, m13 gene v was inserted in a phagemid expression vector and a library of missense and nonsense mutants was constructed by random chemical mutagenesis. phagemids encodi ... | 1992 | 1551382 |
| identification and mapping of origins of dna replication within the dna sequences of the genome of insect iridescent virus type 6. | the origins of dna replication of the genome (209 kbp) of chilo iridescent virus (civ), which is circularly permuted and terminally redundant, were identified. the defined genomic library of civ, which represents 100% of dna sequences of the viral genome (e.g., all 32 ecori civ dna fragments), was used for transfection of choristoneura fumiferana insect cell cultures (cf-124) that were previously infected with civ. the plasmid rescue experiments were carried out to select those recombinant plasm ... | 1992 | 1549908 |
| transmembrane region of wild-type and mutant m13 coat proteins. conformational role of beta-branched residues. | although transmembrane (tm) segments of integral membrane proteins are putatively alpha-helical in conformation, beta-sheet promoters (val, ile, thr) often account for approximately 40% of tm residue composition. we are examining the conformational role(s) of these residues, using as a model system the major coat protein of the filamentous bacteriophage m13. this 50-residue protein, which is located at the escherichia coli host membrane during phage reproduction, contains a prototypic 19-residue ... | 1992 | 1544912 |
| terminating a macromolecular helix. structural model for the minor proteins of bacteriophage m13. | analysis of the results of x-ray diffraction, electron microscopy and s sequence studies of filamentous bacteriophage m13 are used to construct structural models for the minor proteins gp7 and gp9 at the end of the virus assembled first, and a portion of gp6 at the end of the virus that binds host. comparison of the sequence of the major coat protein, gp8, with those of gp7, gp9 and gp6 indicates that significant portions of these three proteins have sequences similar to that of gp8. assuming th ... | 1992 | 1469721 |
| genetic effects of oxidative dna damage: comparative mutagenesis of 7,8-dihydro-8-oxoguanine and 7,8-dihydro-8-oxoadenine in escherichia coli. | a single 7,8-dihydro-8-oxoguanine (g8-oxo; 8-hydroxyguanine) adduct in the lacz alpha gene of bacteriophage m13 dna induces a targeted g-->t transversion after replication in escherichia coli (biochemistry, 29, 7024-7031 (1990)). this mutation is thought to be due to the facile formation during dna synthesis of a g8-oxo.base pair, where g8-oxo is in the syn conformation about the deoxyglycosyl bond. a related modified purine, 7,8-dihydro-8-oxoadenine (a8-oxo; 8-hydroxyadenine), is an abundant pr ... | 1992 | 1461734 |
| identification of meningococcal serosubtypes by polymerase chain reaction. | the polymerase chain reaction was used as the basis of a novel typing method for neisseria meningitidis. southern hybridization experiments demonstrated that it was possible to identify genes encoding different serological variants of the meningococcal class 1 outer membrane protein by probing with polymerase chain reaction products corresponding to known epitopes. a set of 14 defined variable regions was prepared in bacteriophage m13mp19 by the cloning of polymerase chain reaction products. the ... | 1992 | 1452652 |
| purification and properties of dna polymerase from bacillus caldotenax. | a thermostable dna polymerase was prepared from bacillus caldotenax by using a four-step chromatography procedure. the protein exists as a monomer of m(r) 94,000, has a pi of 4.9 and has no associated 3'-5' or 5'-3'-exonuclease activities or endonuclease activity. the temperature optimum of the enzyme was about 70 degrees c and the ph for maximum activity was about 7.5. the enzyme has an absolute requirement for a bivalent cation, and maximum activity was obtained at the unusually high concentra ... | 1992 | 1445254 |
| determination of twin zygosity by dna hybridisation to wild type bacteriophage m13. | 1992 | 1420002 | |
| dna transcription and repressor binding affect deletion formation in escherichia coli plasmids. | chimeric plasmids containing phage m13 and plasmid pbr322 sequences undergo deletions in escherichia coli with a high frequency. in all plasmids one deletion endpoint is the m13 replication origin nick site. we examined the effects of transcription on the position of the other deletion end-point, by inserting in the plasmids an inducible promoter followed by a transcription terminator. transcription dramatically affected deletions in an orientation-dependent way, such that greater than 95% of en ... | 1992 | 1396563 |
| formation of non-bilayer structures induced by m13 coat protein depends on the conformation of the protein. | a comparison is made of the interaction of the coat protein of bacteriophage m13 in a predominant alpha-helix conformation and in a predominant beta-sheet conformation. to perform a systematic study of the interaction between the protein in these two different forms of the surrounding lipid matrix, nmr spectra of 2h-nuclei of specific labelled phospholipid systems are measured. in addition 31p-nmr is employed to provide information about the morphological structure adopted by the reconstituted l ... | 1992 | 1390851 |
| protease inhibitor display m13 phage: selection of high-affinity neutrophil elastase inhibitors. | we report display of the complete protease inhibitor (kunitz) domain, bpti, on the surface of bacteriophage m13 as a fusion to the gene iii product. phage that display bpti bind specifically to anti-bpti antibodies, trypsin and anhydrotrypsin. a point mutation of bpti [lys15-->leu(k15l)] alters the binding specificity of fusion phage such that a human neutrophil elastase-binding phenotype is conferred while a trypsin-binding phenotype is eliminated. phage were eluted from an immobilized protease ... | 1992 | 1385268 |
| corrected nucleotide sequence of m13mp18 gene iii. | there are seven differences between the actual nucleotide (nt) sequence of bacteriophage m13mp18 gene iii and the previously reported nt sequence (which had been compiled based on the nt sequence of wild-type bacteriophage m13 gene iii). | 1992 | 1375182 |
| fab assembly and enrichment in a monovalent phage display system. | we have developed a system that allows the expression of a single copy of an antibody fab molecule on the surface of the filamentous bacteriophage m13 and demonstrate the utility of this system for enrichment of specific "fab phage". a "humanized" version of antibody 4d5 (h4d5) directed against the extracellular domain of the her2 (neu) receptor, was used as prototype to assess the assembly of fab molecules on the phage and to determine the power of the enrichment system. the h4d5 fab phage show ... | 1991 | 1369462 |
| genomic variations in mosquitocidal strains of bacillus sphaericus detected by m13 dna fingerprinting. | the genomic variation of bacillus sphaericus reference and local strains belonging to different serotypes was examined by dna fingerprinting. a phage m13 dna probe detected a number of variable fragments in the restriction digests of total strain dnas. the patterns of band distribution showed a certain homology among mosquitocidal strains, expressed by similarity index d and might be a reliable criterion for assessing the level of genomic similarity between closely related strains. an important ... | 1992 | 1352319 |
| typing of methicillin-resistant staphylococcus aureus with an m13 repeat probe. | a bacteriophage m13 tandem repeat has been used to probe ecori digested genomic dna of methicillin-resistant staphylococcus aureus (mrsa). the patterns generated were found to be useful in typing mrsa and generally confirmed the relationships that had previously been recognized in other studies based on antimicrobial resistance and plasmid profiles. the epidemic mrsa of london hospitals (emrsa) and the majority of the epidemic mrsa of eastern australian hospitals (ea mrsa) gave the same pattern. ... | 1992 | 1350600 |
| cloning, nucleotide sequence, overexpression, and inactivation of the escherichia coli 2-keto-4-hydroxyglutarate aldolase gene. | having previously determined the complete amino acid sequence of 2-keto-4-hydroxyglutarate aldolase from escherichia coli (c. j. vlahos and e. e. dekker, j. biol. chem. 263:11683-11691, 1988), we amplified the gene that codes for this enzyme by the polymerase chain reaction using synthetic degenerate deoxyoligonucleotide primers. the amplified dna was sequenced by subcloning the polymerase chain reaction products into bacteriophage m13; the nucleotide sequence of the gene was found to be in exac ... | 1992 | 1339418 |
| stoichiometry, selectivity, and exchange dynamics of lipid-protein interaction with bacteriophage m13 coat protein studied by spin label electron spin resonance. effects of protein secondary structure. | bacteriophage m13 major coat protein has been isolated with cholate and reconstituted in dimyristoyl- and dioleoylphosphatidylcholine (dmpc and dopc, respectively) bilayers by dialysis. fourier transform infrared spectra of dmpc/coat protein recombinants confirmed that, whereas the protein isolated by phenol extraction was predominantly in a beta-sheet conformation, the cholate-isolated coat protein contained a higher proportion of the alpha-helical conformation [cf. spruijt, r. b., wolfs, c. j. ... | 1992 | 1312343 |
| [preparation of single-stranded e1a-oncogene dna fragments from simian adenovirus sa7 by endonuclease hydrolysis recombinant phage m13 ss-dna complexed with oligonucleotides, containing restriction sites]. | the ss-dna of the (+) and (-) chains of ela dna fragment was obtained by hydrolysis of the recombinant bacteriophages m13 mp8g and mp9g (where g is 1-1750 bp:, e1a region of oncogene sa7) in complexes with the 16 bp oligonucleotides containing alui and bspri sites of restriction and sequences complementary to e1a sa7. the obtained fragments overlap the e1a zones associated with the immortalizing potential of sa7. | 1992 | 1301501 |
| spectroscopy of lipid-protein interactions: structural aspects of two different forms of the coat protein of bacteriophage m13 incorporated in model membranes. | 1992 | 1287668 | |
| cleavage maps of the filamentous bacteriophages m13, fd, fl, and zj/2. | the replicative form dnas of bacteriophage m13, fd, f1, and zj/2 were found to be sensitive to cleavage by the restriction endonucleases endor-hapii, endor-haeii, endor-haeiii, endor-hindii, endor-alui, endor-hha, and endor-hinf. with respect to m13 dna the number of cleavage sites varied from 21 for endor-hinf, 18 for endor-alui, 15 for endor-hha, 13 for endor-hapii, 10 for endor-haeiii, 3 for endor-haeii, to only a single site for endor-hindii. in contrast to m13, fd and f1, the zj/2 dna molec ... | 1976 | 1271528 |
| functional half-lives of bacteriophage m13 gene 5 and gene 8 messages. | the half-lives of the m13 gene 5 and gene 8 messages were determined by measuring the decay in the rate of synthesis of the gene 5 and gene 8 proteins after inhibition of new rna chain initiations with rifampin. the gene 5 and gene 8 messages decay with half-lives of approximately 2.5 and 5 min, respectively. we found no evidence of a functional m13 message with a half-life as long as that reported for hybridizable mrna. | 1976 | 1255878 |
| regulation of gene activity in bacteriophage m13 dna: coupled transcription and translation of purified genes and gene-fragments. | 1975 | 1189286 | |
| studies on the structure of replicative intermediates in bacteriophage m13 single stranded dna synthesis. | pulse-labeled replicative intermediates in m 13 single stranded dna synthesis can be separated by dye-buoyant density centrifugation into two major fractions: supercoiled molecules (ri i) containing viral strands of more than one genome length, and "relaxed" molecules (ri ii) with labeled dna chains shorter than unit length. it is postulated that ri ii molecules might be formed in vivo by site-specific nicking of rf i molecules. | 1975 | 1129143 |
| miniphage-a class of satellite phage to m13. | satellite or defective bacteriophage particles can appear in extensively recycled stocks of coliphage m13. these particles, herein known as miniphage, replicate using the wild type bacteriophage as a helper. their physical properties (u.v. spectra, sedimentation of dna and bacterophage, electrophoretic moblitiy) are described and a method for the isolation of specific satellite bacteriophage is presented. | 1975 | 1123611 |
| physical mapping of the central terminator for transcription on the bacteriophage m13 genome. | with the aid of in vitro transcription and translation studies it has been demonstrated that termination of transcription on bacteriophage m13 replicative form dna occurs at a unique site which is located immediately distal to the 3'-end of gene viii, the gene which codes for the major capsid protein. the position of this site has been mapped accurately on the enzyme cleavage maps by transcription of restriction fragments of m13 rf dna. the central termination site was found to be located in res ... | 1975 | 1103087 |
| effects of bacteriophage m13 infection upon phospholipid and fatty acid compositions of escherichia coli. | escherichia coli k38 were grown and infected with wild type and amber mutants of bacteriophage m13 in the early log phase. lipid compositions of the infected and healthy cultures, grown under identical conditions, were determined 2 hr after infection. from the results, it was observed that total lipid and total phospholipid content remained nearly constant, suggesting that the cell membrane which contained the maximum phospholipids was not damaged by the infection. moreover, the percentage of di ... | 1975 | 1099382 |
| replication of bacteriophage m13 ix. requirement of the escherichia coli dnag function for m13 duplex dna replication. | temperature-shift experiments with an escherichia coli dnag strain indicate a requirement for the dnag function for m13 phage production only at an early stage of infection. mutant cells infected at nonpermissive temperature form the parental rf (ss leads to rf) but do not replicate further. a shift to nonpermissive temperature after infection inhibits rf leads to rf replication but not rf leads to ss synthesis. the synthesis of both strands of the duplex rf was inhibited equally after a tempera ... | 1975 | 1097735 |
| ten proteins required for conversion of phix174 single-stranded dna to duplex form in vitro. resolution and reconstitution. | protein requirements for conversion of phix174 single-stranded dna to a double-stranded replicative form with a small gap (rf ii) have been determined by resolution and reconstitution of the multienzyme system from extracts of gently lysed escherichia coli. assays depended on: (a) complementation of extracts of thermosensitive mutants and (b) fractionation of extracts of wild type cells to divide essential components into groups, each of which was further resolved. these procedures have yielded ... | 1975 | 1097445 |
| studies on bacteriophage m13 dna. 2. the gene order of the m13 genome. | the double-stranded replicative form dna of bacteriophage m13 was cleaved into 13 specific fragments by the restriction endonuclease from haemophilus aphirophilus. the individual dna fragments from wild-type replicative form molecules were then annealed to circular, single-stranded dnas of phage m13, bearing amber mutations as genetic markers. when such dna hybrids infected competent escherichia coli cells, only those duplexes which were genetically heterozygous gave rise to wild-type phages in ... | 1975 | 1095371 |
| identification and characterization of the in vitro synthesized gene products of bacteriophage m13. | bacteriophage m13 replicative form (rf) dna was used to direct coupled transcription and translation in cell-free extracts prepared from escherichia coli. by using rf dna, isolated from cells infected with appropriate amber mutants of this phage, it has been possible to identify the products of genes i through iv. by using the same methods no gene-product relationship could be demonstrated for genes vi and vii. coupled in vitro protein synthesis studies on rf-iii dna, a linear double-stranded dn ... | 1975 | 1089807 |
| studies on bacteriophage m13 dna. 1. a cleavage map of the m13 genome. | a physical map of the bacteriophage m13 genome has been constructed on the basis of specific cleavage of m13 replicative form dna by bacterial restriction endonucleases. the 13 fragments produced by the enzyme from haemophilus aphirophilus (endonuclease r.hap ii) as well as the 10 fragments produced by the enzyme from haemophilus aegyptius (endonuclease r.hae iii) have been ordered by analysis of partial digest products and by analysis of overlapping sets of fragments. in addition, the single si ... | 1975 | 1079769 |
| electron microscopic studies of bacteriophage m13 dna replication. | intracellular forms of m13 phage dna isolated after infection of escherichia coli with wild-type phage have been studied by electron microscopy and ultracentrifugation. the data indicate the involvement of rolling-circle intermediates in single-stranded dna synthesis. in addition to single-stranded circular dna, we observed covalently closed and nicked replicative-form (rf) dnas, dimer rf dnas, concatenated rf dnas, rf dnas with single-stranded tails (theta, rolling circles), and, occasionally, ... | 1977 | 916032 |
| replication of bacteriophage m13. xi. localization of the origin for m13 single-strand synthesis. | 1977 | 845943 | |
| role of hydrophobic forces in membrane protein asymmetry. | m13 virus coat protein is an integral cytoplasmic membrane protein at all stages of viral infection. the pure virus coat protein can also be incorporated into synthetic lecithin vesicles near the lipid-phase transition temperature (tm), spanning the bilayer with its n terminus exposed on the outside and its c-terminus inside (wickner, w. (1976), proc. natl. acad. sci. u.s.a. 73, 1159-1163). the assembly of coat protein into vesicles in this asymmetric fashion has a sharp maximum near the phase-t ... | 1977 | 836786 |
| iolation and charcterization of a dna-binding non-histone protein from tetrahymena pyriformis. | three proteins (a, b and c) that bind specifically to single-stranded dna have been isolated from the eukaryotic organism tetrahymena pyriformis. their molecular weights are 47 000, 41 000 and 32 000. the amino acid composition of the a protein indicates that it is a non-histone protein and sucrose gradient centrifugation shows that it binds to bacteriophage m13 dna and to oligo (dt)100 in a cooperative manner. the exonucleolytic degradation of oligo (dt)100 is prevented when it is bound to the ... | 1975 | 809059 |
| mapping of promoter sites on the genome of bacteriophage m13. | with the aid of transcription studies on restriction fragments of bacteriophage m13 dna it has been demonstrated that at least eight promoter sites are located on the m13 genome. five of these promoters initiate the synthesis of rna chains which contain at their 5'-terminal end pppg (g promoters), while the other three promoters initiate rna chains which start exclusively with pppa (a promoters). the positions of these promoter sites on the physical map are: 0.82 (g0.82), 0.88 (g0.88), 0.94 (g0. ... | 1976 | 795656 |
| bacterial rep- mutations that block development of small dna bacteriophages late in infection. | several related mutants of escherichia coli c have been isolated that block the growth of the small icosahedral dna phages phix174 and s13 late in infection. phage g6 is also blocked, at a stage not yet known. growth of the filamentous phage m13, though not blocked, is affected in these strains. these host mutations co-transduce with ilv at high frequency, as do rep- mutations. however, the new mutants, designated grol-, differ from previously studied rep- mutants in that they permit synthesis o ... | 1976 | 789914 |
| replication of bacteriophage m13. x. m13 replication in a mutant of escherichia coli defective in the 5' leads to 3' exonuclease associated with dna polymerase i. | 1976 | 789894 | |
| restriction-enzyme-cleavage maps of bacteriophage m13. existence of an intergenic region on the m13 genome. | replicative form dna of bacteriophage m13 was cleaved into specific fragments by an endonuclease isolated from hemophilus aegyptius (endor.haeii) and an endonuclease from arthrobacter luteus (endor.alui). the fragments were ordered as to construct a circular map of the phage m13 genome by: (a) using each fragment as a primer for the synthesis in vitro of its respective neighbour and (b) digesting the isolated fragments with the hemophilus aegyptius enzyme endor.haeii or the hemophilus aphirophil ... | 1976 | 786638 |
| the role of escherichia coli dnag function in coliphage m13 dna synthesis. | examination of the role of escherichia coli dnag function in different stages of m13 phage dna synthesis by ultracentrifugal analysis of intracellular phage dna in a thermosensitive dnag mutant shows that: (a) the formation of parental double-strand replicative-form dna (rfdna) from the infecting virus is independent of dnag function; (b) the synthesis of progeny rfdna requires dnag product; (c) after a pool of rfdna is made up, dnag function is not required for the progeny single-strand dna (ss ... | 1976 | 786625 |
| asymmetric orientation of phage m13 coat protein in escherichia coli cytoplasmic membranes and in synthetic lipid vesicles. | at each stage of infection, the major coat protein of coliphage m13 binds to the e. coli cytoplasmic membrane with its antigenic site exposed to the cell exterior [wickner, w. (1975) proc. nat. acad. sci. usa 72, 4749-4753]. this antigenic site is now shown to be at the amino-terminus of the protein. the amino-terminus of m13 coat protein is also found exclusively on the outside of dilauroyl or dimyristoyl lecithin vesicles, formed with coat protein by the cholate dilution technique [racker, e., ... | 1976 | 772680 |
| transcription of bacteriophage m13 dna: existence of promoters directly preceding genes iii, vi, and i. | in vitro transcription and coupled transcription-translation studies have been performed with restriction fragments of bacteriophage m13 replicative-form dna which contain either gene iii, gene vi, or gene i. it could be demonstrated that dna fragments which contain gene iii were able to direct the synthesis of gene iii protein. fragments which encompassed genes vi and i gave rise to the synthesis of gene i protein only, whereas gene i-containing fragments were able to direct the synthesis of ge ... | 1978 | 731795 |
| replication of bacteriophage m13. xiii. structure and replication of cloned m13 miniphage. | 1978 | 731688 | |
| expression of bacteriophage m13 dna in vivo. localization of the transcription initiation and termination signal of the mrna coding for the major capsid protein. | during the infection cycle of the filamentous bacteriophage m13 a phage specific rna species is made which selectively directs in vitro the synthesis of the precursor of the major capsid protein encoded by gene viii. this rna is unstable (its mean half-life is 11 min) and is made in amounts representing at least 2% of the newly synthesized rn. nucleotide sequence analysis have indicated that the synthesis of this rna species is initiated and terminated at the same promoter (g0.18) and terminatio ... | 1978 | 693322 |
| a cascade mechanism of transcription in bacteriophage m13 dna. | 1978 | 664236 |