Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| identification and characterization of cyprinid herpesvirus-3 (cyhv-3) encoded micrornas. | micrornas (mirnas) are a class of small non-coding rnas involved in post-transcriptional gene regulation. some viruses encode their own mirnas and these are increasingly being recognized as important modulators of viral and host gene expression. cyprinid herpesvirus 3 (cyhv-3) is a highly pathogenic agent that causes acute mass mortalities in carp (cyprinus carpio carpio) and koi (cyprinus carpio koi) worldwide. here, bioinformatic analyses of the cyhv-3 genome suggested the presence of non-cons ... | 2015 | 25928140 |
| scales tell a story on the stress history of fish. | fish faced with stressful stimuli launch an endocrine stress response through activation of the hypothalamic-pituitary-interrenal (hpi-) axis to release cortisol into the blood. scientifically validated biomarkers to capture systemic cortisol exposure over longer periods of time are of utmost importance to assess chronic stress in governmental, wildlife, aquaculture and scientific settings. here we demonstrate that cortisol in scales of common carp (cyprinus carpio l.) is the long-sought biomark ... | 2015 | 25922947 |
| optimizing techniques to capture and extract environmental dna for detection and quantification of fish. | few studies have examined capture and extraction methods for environmental dna (edna) to identify techniques optimal for detection and quantification. in this study, precipitation, centrifugation and filtration edna capture methods and six commercially available dna extraction kits were evaluated for their ability to detect and quantify common carp (cyprinus carpio) mitochondrial dna using quantitative pcr in a series of laboratory experiments. filtration methods yielded the most carp edna, and ... | 2016 | 25919417 |
| acute and subchronic toxic effects of atrazine and chlorpyrifos on common carp (cyprinus carpio l.): immunotoxicity assessments. | atrazine (atr) and chlorpyrifos (cpf) are widely used pesticides in agricultural practices throughout world. it has resulted in a series of toxicological and environmental problems, such as impacts on many non-target aquatic species, including fish. the spleen and head kidney in the bony fish are the major hematopoietic organs, and play a crucial part in immune responses. this study evaluated the subchronic effects of atr and cpf on the mrna and protein levels of hsp60, hsp70 and hsp90 in the im ... | 2015 | 25917970 |
| kinetics of lymphocyte subpopulations in allogeneic grafted scales of ginbuna crucian carp. | in mammals the rejection of allografts is primarily accomplished by cell-mediated immunity including t cells. recently, considerable studies reveal the existence of helper and cytotoxic t cell subsets in fish. here we investigate the kinetics of cd4(+) and cd8α(+) t cells along with sigm(+) cells and phagocytic cells in an allogeneic scale graft model using ginbuna crucian carp for understanding the mechanisms of cell-mediated immune response. the results showed that cd4(+) t cells first infiltr ... | 2015 | 25917429 |
| [molecular cloning and expression patterns of two tumor necrosis factor al pha genes in crucian carp (carassius carassius)]. | tumor necrosis factor (tnf) is a potent inflammatory cytokine produced during inflammation. in this study, two crucian carp tnf-alpha genes, tnfalpha-1 and tnfalpha-2 were cloned and sequenced. the tnfalpha-1 was 720 bp and-consisted of a 699 bp opening reading frame encoding 232 amino acids and tnfalpha-2 was 793 bp and contained an open reading frame of 687 bp which encoded 228 amino acids. the genomic structure of both genes consists of4 exons and 3 introns similar to other known tnf-alpha ge ... | 2015 | 25916118 |
| pp2a (pr65) in silver carp: cdna cloning and expression analysis. | the full-length sequence of the protein phosphatase 2a subunit a (pp2a-a) cdna from silver carp was cloned and sequenced. additionally, the transcription of pp2a-a in the liver, kidney, and spleen of fish after microcystin (mc)-exposure were also determined in this study. the results reveal that pp2a-a cdna is 2833 base pair (bp) long and contains an open reading frame of 1767 bp encoding a protein of 589 amino acids. sequence analysis demonstrates that pp2a is highly conserved in fish. furtherm ... | 2015 | 25914120 |
| first evidence of molecular characterization of rohu carp sox2 gene being expressed in proliferating spermatogonial cells. | because little is known about the function of sox2 (sry-related box-2) in teleosts, the objective of this study was to clone and characterize sox2 complementary dna (cdna) from the testis of indian major carp, labeo rohita (rohu). the full-length cdna contained an open reading frame of 936 nucleotides bearing the typical structural features. phylogenetically, sox2 of l rohita was most closely related to freshwater counterparts than marine water. the sequence information of cdna and genomic dna t ... | 2015 | 25913275 |
| improved memetic algorithm based on route distance grouping for multiobjective large scale capacitated arc routing problems. | the capacitated arc routing problem (carp) has attracted considerable attention from researchers due to its broad potential for social applications. this paper builds on, and develops beyond, the cooperative coevolutionary algorithm based on route distance grouping (rdg-maens), recently proposed by mei et al. although mei's method has proved superior to previous algorithms, we discuss several remaining drawbacks and propose solutions to overcome them. first, although rdg is used in searching for ... | 2016 | 25910271 |
| genetic differentiation in indian major carp, cirrhinus mrigala (hamilton, 1822) from indian rivers, as revealed by direct sequencing analysis of mitochondrial cytochrome b region. | a 307 bp segment of cytochrome b gene of mtdna was sequenced and analyzed for 90 individuals of cirrhinus mrigala collected across the three rivers, namely ganges, narmada and brahmaputra. analyses revealed the presence of 14 haplotypes with haplotype diversity (h) ranging from 0.304 to 0.692, and nucleotide diversity (π) 0.002-0.043. the majority of variation was found within the population (96.21%), and the fst value (0.035) as well as the value of exact test of population differentiation (0.8 ... | 2015 | 25909754 |
| mercury in sediment, water, and fish in a managed tropical wetland-lake ecosystem. | mercury pollution has not been well documented in the inland lakes or fishes of mexico, despite the importance of freshwater fish as a source of protein in local diets. total mercury and methylmercury in waters, sediments, and the commercial fish catch were investigated in lake zapotlán, mexico. concentrations of total and methylmercury were very high in runoff and wastewater inputs, but very low in sediments and surface waters of the open water area of the lake. concentrations of total mercury ... | 2015 | 25909268 |
| the first report of dactylogyrus formosus kulwiec, 1927 (monogenea: dactylogyridae) from goldfish (carassius auratus) in central china. | dactylogyrus formosus kulwiec, 1927 (monogenea: dactylogyridae), widely distributed across eurasia, is considered as one of the most frequently encountered ectoparasites of goldfish (carassius auratus). in the present study, d. formosus on gills of cultured goldfish was reported in central china for the first time, using the methods of morphological characters and molecular markers. it was characterized by "anchoratoid-wegeneri" type anchor with elongate inner and unobvious outer root. the morph ... | 2015 | 25899326 |
| sublethal toxicity of quinalphos on oxidative stress and antioxidant responses in a freshwater fish cyprinus carpio. | extensive use of quinalphos, an organophosphorus pesticide, is likely to reach the aquatic environment and thereby posing a health concern for aquatic organisms. oxidative stress and antioxidant responses may be good indicators of pesticide contamination in aquatic organisms. the data on quinalphos induced oxidative stress and antioxidant responses in carps are scanty. this study is aimed to assess the two sublethal concentrations of quinalphos (1.09 and 2.18 μl l(-1) ) on oxidative stress and a ... | 2016 | 25899319 |
| oxidative stress and genotoxicity induced by ketorolac on the common carp cyprinus carpio. | the nonsteroidal anti-inflammatory drug ketorolac is extensively used in the treatment of acute postoperative pain. this pharmaceutical has been found at concentrations of 0.2-60 µg/l in diverse water bodies around the world; however, its effects on aquatic organisms remain unknown. the present study, evaluated the oxidative stress and genotoxicity induced by sublethal concentrations of ketorolac (1 and 60 µg/l) on liver, brain, and blood of the common carp cyprinus carpio. this toxicant induced ... | 2016 | 25899151 |
| monoclonal antibody against m protein of spring viremia of carp virus. | m protein of spring viremia of carp virus (svcv) was expressed in escherichia coli and then used to immunize balb/c mice. one monoclonal antibody (5a1) against m protein was generated by fusion of mouse myeloma cell line sp2/0 and spleen lymphocytes from immunized mice. the characterizations of this mab were confirmed by elisa (enzyme linked immunosorbent assay), ifa (immunofluorescent assay), and western blot analysis. all results indicate that mab 5a1 was specific to svcv m protein. | 2015 | 25897611 |
| sodium cyanide induced alteration in the whole animal oxygen consumption and behavioural pattern of freshwater fish labeo rohita. | sodium cyanide is a common environmental pollutant which is mainly used in many industries such as mining, electroplating, steel manufacturing, pharmaceutical production and other specialized applications including dyes and agricultural products. it enters aquatic environment through effluents from these industries. static renewal bioassay test has been conducted to determine lc, of sodium cyanide on indigenous freshwater carp, labeo rohita. the behavioural pattern and oxygen consumption were ob ... | 2015 | 25895263 |
| carp-1/ccar1: a biphasic regulator of cancer cell growth and apoptosis. | targeted cancer therapy using small molecule inhibitors (smis) has been useful in targeting the tumor cells while sparing the normal cells. despite clinical success of many targeted therapies, their off-target effects and development of resistance are emerging as significant and challenging problems. thus, there is an urgent need to identify targets to devise new means to treat cancers and their drug-resistant phenotypes. carp-1/ccar1 (cell division cycle and apoptosis regulator 1), a peri-nucle ... | 2015 | 25894788 |
| anti-carbamylated protein antibodies in the pre-symptomatic phase of rheumatoid arthritis, their relationship with multiple anti-citrulline peptide antibodies and association with radiological damage. | the presence of a new autoantibody system, anti-carbamylated protein (anti-carp) antibodies, has been identified in rheumatoid arthritis (ra). the presence of anti-carp antibodies was evaluated in samples taken from individuals who subsequently developed ra before and after onset of symptoms and related to previously analysed antibodies against citrullinated peptides (acpa specificities) and anti-ccp2. | 2015 | 25889922 |
| interactions of highly and low virulent flavobacterium columnare isolates with gill tissue in carp and rainbow trout. | the interactions of flavobacterium columnare isolates of different virulence with the gills of carp (cyprinus carpio l.) and rainbow trout (oncorhynchus mykiss walbaum) were investigated. both fish species were exposed to different high (hv) or low virulence (lv) isolates and sacrificed at seven predetermined times post-challenge. histopathological and ultrastructural examination of carp and rainbow trout inoculated with the hv-isolate disclosed bacterial invasion and concomitant destruction of ... | 2015 | 25889257 |
| cyprinid herpesvirus-3 (cyhv-3) disturbs osmotic balance in carp (cyprinus carpio l.)--a potential cause of mortality. | cyprinid herpesvirus-3 (cyhv-3) causes a fatal disease in carp (cyprinus carpio) and its ornamental koi varieties which seriously affects production and trade of this fish species globally. up to now, the pathophysiology of this disease remains unclear. affected individuals develop most prominent lesions in gills, skin and kidney, in tissues which are involved in the osmotic regulation of freshwater teleosts. therefore, here serum and urine electrolyte levels were examined during the course of a ... | 2015 | 25888311 |
| native and non-native plants provide similar refuge to invertebrate prey, but less than artificial plants. | non-native species introductions are widespread and can affect ecosystem functioning by altering the structure of food webs. invading plants often modify habitat structure, which may affect the suitability of vegetation as refuge and could thus impact predator-prey dynamics. yet little is known about how the replacement of native by non-native vegetation affects predator-prey dynamics. we hypothesize that plant refuge provisioning depends on (1) the plant's native status, (2) plant structural co ... | 2015 | 25885967 |
| temperature and species-specific effects on ß3-adrenergic receptor cardiac regulation in two freshwater teleosts: channel catfish (ictalurus punctatus) and common carp (cyprinus carpio). | β₃-adrenergic receptors (ar) are important in teleost cardiovascular regulation. to date, it is unknown whether temperature acclimation changes ß₃-ar functionality and consequently the involvement of this ar subtype in teleost cardiac regulation. common carp (cyprinus carpio) were acclimated at 12 °c or 23 °c (minimum 3 weeks) after which cardiovascular variables (cardiac output (q), stroke volume (sv) and heart rate (fh)) were measured upon injection of the ß₃-ar agonist, brl(37344), and antago ... | 2015 | 25882086 |
| chronic exposure to tributyltin induces brain functional damage in juvenile common carp (cyprinus carpio). | the aim of the present study was to investigate the effect of tributyltin (tbt) on brain function and neurotoxicity of freshwater teleost. the effects of long-term exposure to tbt on antioxidant related indices (mda, malondialdehyde; sod, superoxide dismutase; cat, catalase; gr, glutathione reductase; gpx, glutathione peroxidase), na+-k+-atpase and neurological parameters (ache, acetylcholinesterase; mao, monoamine oxidase; no, nitric oxide) in the brain of common carp were evaluated. fish were ... | 2015 | 25879203 |
| evaluation of an extended-release formulation of ceftiofur crystalline-free acid in koi (cyprinus carpio). | the use of an extended release ceftiofur crystalline-free acid formulation (ccfa, excede for swine(®) , pfizer animal health) in koi was evaluated after administration of single intramuscular (i.m.) or intracoelomic (i.c.) doses. twenty koi were divided randomly into a control group and four treatment groups (20 mg/kg i.m., 60 mg/kg i.m., 30 mg/kg i.c., and 60 mg/kg i.c.). serum ceftiofur-free acid equivalents (cfae) concentrations were quantified. the pharmacokinetic data were analyzed using a ... | 2015 | 25876672 |
| bioaccumulative characteristics of tetrabromobisphenol a and hexabromocyclododecanes in multi-tissues of prey and predator fish from an e-waste site, south china. | tetrabromobisphenol a (tbbpa) and hexabromocyclododecanes (hbcds) were analyzed in 12 tissues of prey (mud carp) and predator (northern snakehead) fish from an e-waste area, south china. the tbbpa concentrations in different tissues ranged from 0.03 to 2.85 ng/g wet weight (ww) in mud carp and 0.04 to 1.30 ng/g ww in northern snakehead. the concentrations of hbcds ranged from 0.07 to 96.9 ng/g ww in mud carp and 0.18 to 240 ng/g ww in northern snakehead. hbcd levels in tissues were correlated wi ... | 2015 | 25874430 |
| [the effect of magnetic fields on the activity of proteinases and glycosidases in the intestine of the crucian carp carassius carassius]. | it has been demonstrated by the example of the crucian carp (carassius carassius) that a 1-hour stay of fish in a combined magnetic field with resonance parameters for calcium ions decreases the proteolytic and amylolytic activities of their intestinal enzymes. it has been found that a 1-hour exposure to a combined magnetic field with resonance parameters for potassium ions has almost no effect on the activity of proteinases, but it decreases the amylolytic activity. it has been noted that the a ... | 2016 | 25872402 |
| transcriptome analysis of the plateau fish (triplophysa dalaica): implications for adaptation to hypoxia in fishes. | triplophysa dalaica, endemic species of qinghai-tibetan plateau, is informative for understanding the genetic basis of adaptation to hypoxic conditions of high altitude habitats. here, a comprehensive gene repertoire for this plateau fish was generated using the illumina deep paired-end high-throughput sequencing technology. de novo assembly yielded 145, 256 unigenes with an average length of 1632 bp. blast searches against genbank non-redundant database annotated 74,594 (51.4%) unigenes encodin ... | 2015 | 25869933 |
| enantioselective analysis of non-steroidal anti-inflammatory drugs in freshwater fish based on microextraction with a supramolecular liquid and chiral liquid chromatography-tandem mass spectrometry. | toxicity of pharmaceuticals to aquatic biota is still largely unknown, and no research on the stereoselective toxicity of chiral drugs to these organisms has been undertaken to date. because of the lack of analytical methods available for this purpose, this manuscript deals, for the first time, with the enantioselective analysis of the non-steroidal anti-inflammatory drugs (nsaids) ibuprofen, naproxen and ketoprofen in freshwater fish. the method was based on the microextraction of nsaids from f ... | 2015 | 25869485 |
| alkylphenol ethoxylates and brominated flame retardants in water, fish (carp) and sediment samples from the vaal river, south africa. | alkylphenol ethoxylates (apes) and brominated flame retardants (bfrs) are known to be bio-accumulative, persistent, and endocrine disruptors and can cause adverse health effects in animals and humans. in this study, environmental samples were collected from sites along the vaal river, south africa in order to determine the concentrations of apes and bfrs in water, sediment, and fish samples. the highest concentrations of these pollutants were observed from discharge of the rietspruit wwtw. measu ... | 2015 | 25869432 |
| involvement of the mitfa gene in the development of pigment cell in japanese ornamental (koi) carp (cyprinus carpio l.). | a colored phenotype is an important feature of ornamental fish. in mammals, microphthalmia-associated transcription factor (mitf) was found to regulate the development of melanocytes. in this study, the mitfa cdna was first cloned from the japanese ornamental (koi) carp (cyprinus carpio l.), an important ornamental freshwater fish. the full-length cdna of the mitfa gene contains 1634 bp, coding for 412 amino acids in koi. the identity degree of mitfa amino acid sequences between the koi carp and ... | 2015 | 25867426 |
| adaptive expression of uncoupling protein 1 in the carp liver and kidney in response to changes in ambient temperature. | the expression of uncoupling protein (ucp1) is up-regulated in mammalian brown adipocytes during cold exposure. however, a previous study revealed that ucp1 was highly expressed in the liver of common carps, and that the hepatic expression of ucp1 was down-regulated during cold exposure. the present study examined the effects of temperature on the recovery of ucp1 expression levels and the expression of genes involved in ucp1 transcription in the livers and kidneys of common carps. the hepatic a ... | 2015 | 25865906 |
| benzo(a)pyrene-induced cytochrome p4501a expression of four freshwater fishes (oryzias latipes, danio rerio, cyprinus carpio, and zacco platypus). | oryzias latipes, danio rerio, cyprinus carpio, and zacco platypus are useful indicator species for cyp1a biomarker studies; however, comparative studies have not been performed. to compare susceptibility, dose- and time-dependent cyp1a induction at the mrna and protein levels in response to benzo(a)pyrene (bap) exposure was analyzed. at the mrna level, a statistically significant difference was found among the four species; however, such was not observed at the protein level. c. carpio showed th ... | 2015 | 25863331 |
| in vitro antioxidant and hepatoprotective potential of azolla microphylla phytochemically synthesized gold nanoparticles on acetaminophen - induced hepatocyte damage in cyprinus carpio l. | the present study aims to evaluate the hepatoprotective and antioxidant effects of gold nanoparticles (gnap) biosynthesized through the mediation of azolla microphylla and a. microphylla extract on acetaminophen-induced hepatocyte damage in common carp fish (cyprinus carpio l.). the gold nanoparticles (100, 150, 200 μg/ml) and a. microphylla extract powder (100, 200, 400 μg/ml) were added to the primary hepatocytes in different conditions: treatment i (before 12 mm acetaminophen), treatment ii ( ... | 2015 | 25862331 |
| description of myxobolus balatonicus n. sp. (myxozoa: myxobolidae) from the common carp cyprinus carpio l. in lake balaton. | myxobolus balatonicus n. sp. was detected in the gill filaments of the common carp cyprinus carpio l. collected in lake balaton, hungary. its oval plasmodia measuring 600-800 × 300-400 µm were located intravasally in the filamental arteries. the spores measured 11.2 ± 0.92 × 9.5 ± 0.41 × 7.4 ± 0.33 µm and had two equal polar capsules with six filamental turns. both morphology and dna sequence analysis revealed that m. balatonicus n. sp. is distinct from the ten species of myxobolus bütschli, 188 ... | 2015 | 25862034 |
| azadirachtin, a neem-derived biopesticide, impairs behavioral and hematological parameters in carp (cyprinus carpio). | azadirachtin (aza) is a promisor biopesticide used in organic production and aquaculture. although this compound is apparently safe, there is evidence that it may have deleterious effects on fish. behavioral and hematological tests are grouped into a set of parameters that may predict potential toxicity of chemical compounds. here, we investigate the effects of aza, in the commercial formulation neenmax(™) , on carp (cyprinus carpio) by defining lc50 (96 h), and testing behavioral and hematologi ... | 2016 | 25847134 |
| asian fish tapeworm, khawia japonensis (yamaguti, 1934), has expanded its european invasive range. | the invasive fish tapeworm, khawia japonensis (yamaguti, 1934) originally described in japan, is reported for the first time in slovakia. the tapeworm was found in farmed common carp (cyprinus carpio l.) from east-slovakian breeding fish pond. this finding is registered 4 years after the first announcement of this parasite in europe (po river basin in italy) in 2010 and increases its distribution area within this continent. morphological characterization of k. japonensis supplemented with dna se ... | 2015 | 25843570 |
| identification and rapid diagnosis of the pathogen responsible for haemorrhagic disease of the gill of allogynogenetic crucian carp. | in recent years, an epizootic causing severe mortality among allogynogenetic crucian carp (acc), designated as haemorrhagic disease of acc gill, occurred in yancheng city of jiangsu province of china. obvious haemorrhage in the gills of moribund fish and a mortality rate of 100% were observed when accs were artificially infected with liver homogenate from diseased fish. a herpes-like virus, with enveloped virions ranged from 170 to 220 nm in diameter, could be observed in the tissues of challeng ... | 2015 | 25839411 |
| fish peptone development using enzymatic hydrolysis of silver carp by-products as a nitrogen source in staphylococcus aureus media. | fish peptone was produced using enzymatic hydrolysis of silver carp filleting by-products by alcalase and trypsin. also, the efficiency of the hydrolysates as a nitrogen source in staphylococcus aureus medium was compared with commercial tsb. the results indicated that the protein hydrolysate from alcalase and trypsin had high protein content (92.92%, 91.53 respectively), and degree of hydrolysis (4.94%, 4.6% respectively).the results showed that silver carp filleting waste can be an efficient s ... | 2015 | 25838893 |
| comparison of metal concentrations in the organs of two fish species from the zabol chahnimeh reservoirs, iran. | this study was conducted to measure the cd, pb, cu, zn, cr, co, ni, and fe concentrations in organs of cyprinus carpio and hypophthalmichthys molitrix, in zabol chahnimeh reservoirs, iran. the highest pb, cu, co, ni, and fe concentrations in both fish species were observed in the gill of c. carpio at 0.70, 3.7, 0.7, 0.8 and 31.2 µg/g, respectively; while the highest concentrations of cd, zn, and cr were found in the gill of h. molitrix at 0.13, 22.5, and 0.5 µg/g, respectively. the maximum allow ... | 2015 | 25836264 |
| development of an improved rt-pcr for specific detection of spring viraemia of carp virus. | spring viraemia of carp (svc) is a rhabdovirus infection, which has a significant economic impact in pond cultures of carp in europe and western independent states of the former soviet union. the causative agent of svc, spring viraemia of carp virus (svcv), has been divided into four subgroups, ia, ib, ic and id, on the basis of glycoprotein (g) protein gene sequences. in this study, a new primer set was designed from a g gene sequence of svcv to identify the four subtypes of svcv by reverse tra ... | 2016 | 25832786 |
| [polychlorinated biphenyls and their methylsulfonyl metabolites in fish from an electronic waste recycling site in south china: tissue distribution and human dietary exposure]. | in the present study, we determined polychlorinated biphenyls and their methylsulfonyl metabolites (meso2-pcbs) in the tissues of two fish species-mud carp and northern snakehead-from a natural pond in an electronic waste recycling site in the pearl river delta, southern china. the mean concentrations of pcbs (σ pcbs )varied from 560 to 10 462 ng x g(-1) wet weight, and from 580 to 50 492 ng x g(-1) wet weight in the tissues of mud carp and northern snakehead, respectively, with the highest leve ... | 2014 | 25826938 |
| facile and eco-friendly synthesis of green fluorescent carbon nanodots for applications in bioimaging, patterning and staining. | we report a facile and eco-friendly strategy for the fabrication of green fluorescent carbon nanodots (cds), and demonstrate their applications for bio-imaging, patterning, and staining. a one-pot hydrothermal method using various plant petals yields bright green-emitting cds, providing an easy way for the production of green fluorescent cds without the need for a tedious synthetic methodology or the use of toxic/expensive solvents and starting materials. the as-prepared cds show small size dist ... | 2015 | 25826612 |
| development and evaluation of a database of dietary bioaccumulation test data for organic chemicals in fish. | dietary bioaccumulation tests for fish have been conducted for about 40 years. standardized test guidance has recently been developed. test metrics of primary scientific and regulatory interest are the whole body depuration rate constant (kt), whole body growth corrected depuration rate constant (ktg), and corresponding chemical half-lives (t1/2 and t1/2g), dietary chemical absorption efficiency (ae), and biomagnification factor (bmf). a database of 3032 measurement end points for 477 discrete o ... | 2015 | 25821900 |
| genomic variation in the hybrids of white crucian carp and red crucian carp: evidence from ribosomal dna. | in this study, we conducted a cross of white crucian carp (♀)×red crucian carp (♂) (wr), and characterized the morphology, reproduction and genetics of the progeny. different from parents, wr with the gray color showed the hybrid morphological traits of both parents. wr possessed normal gonads producing mature eggs or sperm, and exhibited high fertilization rate (90.2%) and high hatchery rate (80.5%), which contributed to produce and enlarge the population. wr with the same dna content as parent ... | 2015 | 25804929 |
| de novo assembly and characterization of the spleen transcriptome of common carp (cyprinus carpio) using illumina paired-end sequencing. | common carp (cyprinus carpio) is one of the most important aquacultured species of the family cyprinidae, and breeding this species for disease resistance is becoming more and more important. however, at the genome or transcriptome levels, study of the immunogenetics of disease resistance in the common carp is lacking. in this study, 60,316,906 and 75,200,328 paired-end clean reads were obtained from two cdna libraries of the common carp spleen by illumina paired-end sequencing technology. total ... | 2015 | 25804493 |
| fat-associated lymphoid cluster in cyprinus carpio: characterisation and its relation with peritoneal haemangiosarcoma. | falc cells are natural helper cells producing th2-type cytokines, which express c-kit, sca-1, il7r and cd45 in mouse and human. these cells are involved in allergic responses and contribute to the inflammatory reactions of adipose tissue; however, a lack of information prevails about the presence of these cells in other species. the aim of the study was to identify and characterise falc cells in the common carp (cyprinus carpio) using immunohistochemistry and molecular biology techniques as well ... | 2015 | 25804491 |
| maintaining economic value of ecosystem services whilst reducing environmental cost: a way to achieve freshwater restoration in china. | freshwater fisheries are central to food security in china and this remains one of the most important priorities for the growing human population. thus, combining ecosystem restoration with economics is pivotal in setting successful conservation in china. here, we have developed a practical management model that combines fishery improvement with conservation. for six years, a ban on fertilizer and a reduction of planktivorous fish stocking along with the introduction of both mandarin fish sinipe ... | 2015 | 25803696 |
| development of a regulatory testing procedure to study the metabolism of pesticides in farmed fish. | diets used in commercial fish farming use significant proportions of crop-derived commodities, and it is important to understand the potential for transfer of any pesticide residues on the crop into edible tissues in fish. it is a current requirement in the eu that fish metabolism studies must be performed when a pesticide is used in crops where commodities or processed fractions are fed to farmed fish. fish metabolism studies in both rainbow trout and common carp have been carried out, followin ... | 2016 | 25800530 |
| use of droplet digital pcr for estimation of fish abundance and biomass in environmental dna surveys. | an environmental dna (edna) analysis method has been recently developed to estimate the distribution of aquatic animals by quantifying the number of target dna copies with quantitative real-time pcr (qpcr). a new quantitative pcr technology, droplet digital pcr (ddpcr), partitions pcr reactions into thousands of droplets and detects the amplification in each droplet, thereby allowing direct quantification of target dna. we evaluated the quantification accuracy of qpcr and ddpcr to estimate speci ... | 2015 | 25799582 |
| a mathematical model of the carp heart ventricle during the cardiac cycle. | the poikilothermic heart has been suggested as a model for studying some of the mechanisms of early postnatal mammalian heart adaptations. we assessed morphological parameters of the carp heart (cyprinus carpio l.) with diastolic dimensions: heart radius (5.73mm), thickness of the compact (0.50mm) and spongy myocardium (4.34mm), in two conditions (systole, diastole): volume fraction of the compact myocardium (20.7% systole, 19.6% diastole), spongy myocardium (58.9% systole, 62.8% diastole), trab ... | 2015 | 25797310 |
| erratum to: anti-carp antibodies as promising marker to measure joint damage and disease activity in patients with rheumatoid arthritis. | 2015 | 25796173 | |
| biomass and abundance biases in european standard gillnet sampling. | the european standard en 14757 recommends gillnet mesh sizes that range from 5 to 55mm (knot-to-knot) for the standard monitoring of fish assemblages and suggests adding gillnets with larger mesh sizes if necessary. our research showed that the recommended range of mesh sizes did not provide a representative picture of fish sizes for larger species that commonly occur in continental europe. we developed a novel, large mesh gillnet which consists of mesh sizes 70, 90, 110 and 135mm (knot to knot, ... | 2015 | 25793776 |
| molecular characterization of shewanella and aeromonas isolates associated with spoilage of common carp (cyprinus carpio). | storage in ice is a common way of preserving commercial fish species but some microorganisms can still contaminate and participate in the spoilage of the product; therefore, identification of potential harmful microbes is important. thirteen colonies were isolated from common carp (cyprinus carpio) that had been stored in ice, whose phenotypic identification revealed that they belonged to the genera aeromonas (n = 5) and shewanella (n = 8). molecular genotyping with eric-pcr showed clonality onl ... | 2015 | 25790506 |
| collagen/chitosan film containing biotinylated glycol chitosan nanoparticles for localized drug delivery. | the objective of this study was to design a drug delivery system consisting of biotinylated cholesterol-modified glycol chitosan (bio-chgc) nanoparticles and fish collagen/chitosan (col/ch) film for localized chemotherapy. bio-chgc was synthesized, and then its self-assembled nanoparticles were prepared by probe sonication. doxorubicin (dox)-loaded bio-chgc (dbc) nanoparticles prepared by dialysis had spherical shape, and their sizes were in the range of 330-397 nm. col/ch/dbc nanoparticle films ... | 2015 | 25784300 |
| integrated assessment of biomarker response in carp (cyprinus carpio) and silver catfish (rhamdia quelen) exposed to clomazone. | clomazone is considered a potential contaminant of groundwater and is persistent in the environment. to verify the effects of clomazone in cyprinus carpio and rhamdia quelen, a method that combines biomarker responses into an index of "integrated biomarker response" (ibr) was used for observed biological alterations in these species. thiobarbituric acid-reactive substances in liver of carp and silver catfish decreased at both concentrations tested. however, in muscle it increased in carp at 3 mg ... | 2015 | 25779373 |
| variations in temperature acclimation effects on glycogen storage, hypoxia tolerance and swimming performance with seasonal acclimatization in juvenile chinese crucian carp. | the aim of this study was to test whether temperature acclimation (10 vs 20 °c) effects on tissue glycogen content, hypoxia tolerance, and swimming performance of chinese crucian carp (carassius auratus) varied with seasonal acclimatization (winter vs spring) and potential combined interactions. both the routine metabolic rate (mo(2rout)) and critical oxygen tension (p(crit)) of the mo(2rout) increased significantly with temperature, whereas the seasonal acclimatization showed no significant eff ... | 2015 | 25776929 |
| sterile 'judas' carp--surgical sterilisation does not impair growth, endocrine and behavioural responses of male carp. | use of 'judas' fish to betray the locations of conspecifics is a powerful tool in management of invasive pest fish but poses a risk of contributing to recruitment. our aim therefore was to generate surgically sterilised male common carp (cyprinus carpio) and test whether they readily assimilate into wild populations, retain sexual behaviour and successfully betray the locations of feral carp. male common carp were surgically sterilised (n=44) adopting a two-point nip technique, using either a ha ... | 2015 | 25776462 |
| [seasonal fluctuations of migratory activity of vertebrate nuclear hemocytes at different incubation temperatures]. | agarose migration test has shown that nuclear erythrocytes of cyprinus carpio, rana ridibunda, and gallus domesticus are capable for spontaneous locomotions. the migration of red blood cells from frogs is associated with formations of long pseudopodia, whereas that from carps and hens--with short protrusions. it has been shown that migratory activity of nuclear erythrocytes and leukocytes from rana ridibunda and gallus domesticus under effect of temperature in vitro had seasonal nature, while th ... | 2015 | 25775857 |
| identification and comparison of gonadal transcripts of testis and ovary of adult common carp cyprinus carpio using suppression subtractive hybridization. | the limited number of gonad-specific and gonad-related genes that have been identified in fish represents a major obstacle in the study of fish gonad development and sex differentiation. in common carp cyprinus carpio from china's yellow river, the ovary and testis differ in volume and weight in adult fish of the same age. comparing sperm, egg, and somatic cell transcripts in this carp may provide insight into the mechanisms of its gonad development and sex differentiation. in the present work, ... | 2015 | 25772851 |
| effect of semen extender supplementation with cysteine on postthaw sperm quality, dna damage, and fertilizing ability in the common carp (cyprinus carpio). | amino acids have an important biological role for prevention of cell damage during cryopreservation. the objective of this study is to determine the effects of cysteine on postthaw sperm motility, duration of sperm motility, dna damage, and fertility in the common carp (cyprinus carpio). sperm collected from 10 individuals was cryopreserved in extenders containing different cysteine concentrations (2.5, 5, 10, and 20 mm). semen samples diluted at the ratio of 1:9 by the extenders were subjected ... | 2015 | 25772850 |
| targeted inhibition of ankrd1 disrupts sarcomeric erk-gata4 signal transduction and abrogates phenylephrine-induced cardiomyocyte hypertrophy. | accumulating evidence suggest that sarcomere signalling complexes play a pivotal role in cardiomyocyte hypertrophy by communicating stress signals to the nucleus to induce gene expression. ankyrin repeat domain 1 (ankrd1) is a transcriptional regulatory protein that also associates with sarcomeric titin; however, the exact role of ankrd1 in the heart remains to be elucidated. we therefore aimed to examine the role of ankrd1 in cardiomyocyte hypertrophic signalling. | 2015 | 25770146 |
| development of a hypoallergenic recombinant parvalbumin for first-in-man subcutaneous immunotherapy of fish allergy. | the fast (food allergy-specific immunotherapy) project aims at developing safe and effective subcutaneous immunotherapy for fish allergy, using recombinant hypoallergenic carp parvalbumin, cyp c 1. | 2015 | 25765512 |
| distribution and accumulation of polycyclic aromatic hydrocarbons (pahs) in the food web of nansi lake, china. | the concentrations of polycyclic aromatic hydrocarbons (pahs) were analyzed in water, sediment, and biota (aquatic plant, shrimp, and fish) of nansi lake by gas chromatography-mass spectrometry (gc-ms). the concentrations of total pahs were 27.54-55.04 ng l(-1) in water, 80.31-639.23 ng g(-1) dry weight (dw) in sediments, 20.92-192.78 ng g(-1) dw in aquatic plants, and 67.3-533.9 ng g(-1) dw in fish and shrimp muscles. the ratios of phenanthrene to anthracene (ph/an), fluoranthene to pyrene (flu ... | 2015 | 25762425 |
| physiological and molecular responses in brain of juvenile common carp (cyprinus carpio) following exposure to tributyltin. | tributyltin (tbt), as antifouling paints, is widely present in aquatic environment, but little is known regarding the toxicity of tbt on fish brain. in this study, the effects of exposure to tbt on the antioxidant defense system, na(+) -k(+) -atpase activity, neurological enzymes activity and hsp 70 protein level in brain of juvenile common carp (cyprinus carpio) were studied. fish were exposed to sublethal concentrations of tbt (5, 10 and 20 μg/l) for 7 days. based on the results, with increasi ... | 2016 | 25761124 |
| reorganization of low-molecular-weight fraction of plasma proteins in the annual cycle of cyprinidae. | reorganization of the low-molecular-weight fraction of cyprinid plasma was analyzed using various electrophoretic techniques (disc electrophoresis, electrophoresis in polyacrylamide concentration gradient, in polyacrylamide with urea, and in sds-polyacrylamide). the study revealed coordinated changes in the low-molecular-weight protein fractions with seasonal dynamics and related reproductive rhythms of fishes. we used cultured species of the cyprinidae family with sequenced genomes for the dete ... | 2015 | 25756535 |
| effects of prometryne on early life stages of common carp (cyprinus carpio l.). | toxicity of prometryne to early life stages of common carp was assessed. on the basis of accumulated mortality in the experimental groups lowest observed-effect concentration (loec) was estimated as 1100 µg/l; and no observed-effect concentration (noec) was 850 µg/l. fulton's condition factor was significantly lower than in controls in fish exposed to 4000 µg/l after 7, 14, and 21 days. by day 14, fish exposed to 4000 µg/l prometryne showed significantly lower mass and total length compared to c ... | 2015 | 25752431 |
| expression of immune-related genes of common carp during cyprinid herpesvirus 3 infection. | fish herpesviruses and their hosts may have coevolved for 400 to 450 million yr. during this coexistence, the hosts have equipped themselves with an elaborate immune system to defend themselves from invading viruses, whereas the viruses have developed strategies to evade host immunity, including the expression of cytokine genes that have been captured from the host. taking advantage of our experimental model for cyprinid herpesvirus 3 (cyhv-3) persistence in carp, we studied the gene expression ... | 2015 | 25751855 |
| effects of the lipid regulating drug clofibric acid on pparα-regulated gene transcript levels in common carp (cyprinus carpio) at pharmacological and environmental exposure levels. | in mammals, the peroxisome proliferator-activated receptor α (pparα) plays a key role in regulating various genes involved in lipid metabolism, bile acid synthesis and cholesterol homeostasis, and is activated by a diverse group of compounds collectively termed peroxisome proliferators (pps). specific pps have been detected in the aquatic environment; however little is known on their pharmacological activity in fish. we investigated the bioavailability and persistence of the human pparα ligand c ... | 2015 | 25749508 |
| correction: the muscle ankyrin repeat proteins carp, ankrd2, and darp are not essential for normal cardiac development and function at basal conditions and in response to pressure overload. | 2015 | 25747160 | |
| alteration in the cytokine levels and histopathological damage in common carp induced by glyphosate. | glyphosate is one of the most frequently used herbicides, and it has been demonstrated to generate a series of toxicological problems in animals and humans. however, relatively little is known about the effects of glyphosate on the immune system of fish. in the present study, the acute toxicity of glyphosate on common carp was first determined; then, the contents of interferon-γ (ifn-γ), interleukin-1β (il-1β), and tumor necrosis factor -α (tnf-α) and histopathological alterations in the liver, ... | 2015 | 25747155 |
| optimization of demineralization on cyprinus carpio haematopterus scale by response surface methodology. | fish scale, separated during fish mechanical processing, can serve as an additional source of proteins, especially of collagen proteins. to obtain high purity native collagen, it is required to carry out deproteinization and demineralization of fish scales. therefore, the aim of this study was to determine the optimal conditions for demineralization of cyprinus carpio haematopterus scale without loss of collagen content in hcl solution. here, the demineralization of cyprinus carpio haematopterus ... | 2015 | 25745241 |
| effect of blend ratio and ph on the physical properties of edible composite films prepared from silver carp surimi and skin gelatin. | the effect of blend ratio and ph on the physical properties of surimi-gelatin composite films was investigated. tensile strength (ts), film water solubility and soluble proteins of composite films increased with the increasing proportion of gelatin, while elongation at break (eab) decreased. the ts of neutral films with the same ratio of surimi and gelatin were lowest, while increased at acidic or alkaline conditions. similar tendency was also found in protein solubility and surface hydrophobici ... | 2015 | 25745232 |
| study on heavy metal levels and its health risk assessment in some edible fishes from nansi lake, china. | eight heavy metals, namely cu, zn, fe, mn, cd, ni, pb, and as in the muscles of nine fish species collected from nansi lake, china. were determined, and the potential health risks to local residents via consumption of the fishes were estimated. the results of two-way anova that showed the concentrations of heavy metals in the investigated fish samples were influenced significantly by fish species and sampling sites. correlation analysis indicated that sampling sites had significant effects on th ... | 2015 | 25740685 |
| evaluation of the potential for trophic transfer of roxithromycin along an experimental food chain. | pharmaceuticals have been recognized as a new class of environmental pollutants in recent years. but data about their potential for transfer and biomagnification in aquatic food chains are still lacking. in this study, bioaccumulation of the macrolide antibiotic roxithromycin (rox) was determined in an experimental aquatic food chain involving the green algae scenedesmus obliquus, the water flea daphnia magna and the crucian carp carassius auratus. after 48 h of exposure, s. obliquus accumulated ... | 2015 | 25739841 |
| base composition of the danio chrysotaenitus complete mitochondrial genome. | in this study, 16 sets of primers were used to amplify contiguous, overlapping segments of the complete mitochondrial dna (mtdna) of danio chrysotaenitus in order to characterize and compare their mitochondrial genomes. the total length of the mitochondrial genome is 16,608 bp and deposited in the genbank with accession numbers kp407138. the organization of the mitochondrial genomes was similar to those reported from other mountain carp fishes mitochondrial genomes containing 37 genes (13 protei ... | 2016 | 25738218 |
| rapid detection of cyprinid herpesvirus-3 (cyhv-3) using a gold nanoparticle-based hybridization assay. | cyprinid herpesvirus-3 (cyhv-3) is a highly infectious pathogen that causes fatal disease in common and koi carp cyprinus carpio l. cyhv-3 detection is usually based on virus propagation or amplification of the viral dna using the pcr or lamp techniques. however, due to the limited susceptibility of cells used for propagation, it is not always possible to successfully isolate cyhv-3 even from tissue samples that have high virus titres. all previously described detection methods including pcr-bas ... | 2015 | 25738211 |
| [the effects of geomagnetic storms on proteinase and glycosidase activities in fish intestinal mucosa]. | it has been demonstrated that the glycosidase activity of cyprinoid fishes (carp and crucian carp) exposed to a geomagnetic storm for up to 20 h considerably decreases; however, the proteinase activity is weakly altered (a statistically significant decrease in the enzyme activity has been observed only in fasting fish). an in vitro study of the effects of individual half hour intervals of the geomagnetic storm that correspond to the main and recovery phases on the same enzyme activities demonstr ... | 2016 | 25735168 |
| polymorphism analysis of the intron one of insulin-like growth factor 2 receptor gene (igf2r) in ffrc strain common carp (cyprinus carpio l.) and its relationship with growth performance. | the insulin-like growth factor 2 receptor gene (igf2r) encodes a transmembrane protein receptor and acts to sequester and degrade excess circulating insulin-like growth factor 2, which is critical for normal mammalian growth and development. thus, igf2r may serve as a candidate gene underlying growth trait in the common carp. in this study, we isolated the intron one of common carp igf2r and detected the diversity in 3 continuous generations of ffrc strain common carp. a total of 8 loci were det ... | 2015 | 25729973 |
| hypoxia tolerance, nitric oxide, and nitrite: lessons from extreme animals. | among vertebrates able to tolerate periods of oxygen deprivation, the painted and red-eared slider turtles (chrysemys picta and trachemys scripta) and the crucian carp (carassius carassius) are the most extreme and can survive even months of total lack of oxygen during winter. the key to hypoxia survival resides in concerted physiological responses, including strong metabolic depression, protection against oxidative damage and-in air-breathing animals-redistribution of blood flow. each of these ... | 2015 | 25729057 |
| cue-based and algorithmic learning in common carp: a possible link to stress coping style. | common carp that had been screened for stress coping style using a standard behavioural test (response to a novel environment) were given a learning task in which food was concealed in one of two compartments, its location randomised between trials and its presence in a given compartment signalled by either a red or a yellow light. all the fish learned to find food quickly, but did so in different ways. fifty five percent learned to use the light cue to locate food; the remainder achieved the sa ... | 2015 | 25725347 |
| integrative taxonomy of central european parasitic flatworms of the family prosthogonimidae lühe, 1909 (trematoda: plagiorchiida). | species of the family prosthogonimidae are considered the most pathogenic poultry trematodes worldwide, affecting particularly low intensity farming in rural areas. adults of prosthogonimus occur mainly in the bursa of fabricius, oviduct and cloaca of ducks, geese, fowl and other birds feeding at least occasionally on dragonflies or damselflies (odonata). we analyzed the central european species of the prosthogonimidae, namely prosthogonimus cuneatus, prosthogonimus ovatus, prosthogonimus pelluc ... | 2015 | 25724855 |
| effect of dietary arginine on the immune response and gene expression in head kidney and spleen following infection of jian carp with aeromonas hydrophila. | this study was conducted to test the hypothesis that elevated dietary arginine enhances immunity of juvenile jian carp (cyprinus carpio var. jian). fish were fed graded levels of dietary arginine for 9 weeks. subsequently, a challenge test was conducted by injection of aeromonas hydrophila. head kidney and spleen weights, as well as erythrocyte and leukocyte counts were significantly influenced by dietary arginine levels. a similar trend was also observed for hemagglutination titre, serum lysozy ... | 2015 | 25721332 |
| establishment and characterization of fin cell lines from diploid, triploid, and tetraploid oriental weatherfish (misgurnus anguillicaudatus). | continuous fin cell lines from diploid, triploid, and tetraploid oriental weatherfish, misgurnus anguillicaudatus, were established and characterized. the cell lines, designated dimf, trmf, and temf, respectively, were subcultured more than 80 times since initiation in october 2012 and were preserved at the china center for type culture collection as sample numbers c2013109, c2013110, c2013111, respectively. the cell lines consist predominantly of fibroblast-like cells. at the 50th passage, the ... | 2015 | 25721100 |
| [investigation of membrane permeability of carp spermatozoa for water molecules]. | the fundamentals of a photometry method for determination of membrane permeability of some fish spermatozoa for water molecules are presented. osmotic tolerance of carp spermatozoa membranes was studied using epr-spectroscopy and photometric analysis methods. it was shown that carp spermatozoa look like the ideal osmometers in their reaction on media of different osmolarity. the value of membrane permeability of carp spermatozoa for water molecules was determined. data obtained can be used in cr ... | 2015 | 25715589 |
| phosphorylation-independent suppression of light-activated visual pigment by arrestin in carp rods and cones. | visual pigment in photoreceptors is activated by light. activated visual pigment (r*) is believed to be inactivated by phosphorylation of r* with subsequent binding of arrestin. there are two types of photoreceptors, rods and cones, in the vertebrate retina, and they express different subtypes of arrestin, rod and cone type. to understand the difference in the function between rod- and cone-type arrestin, we first identified the subtype of arrestins expressed in rods and cones in carp retina. we ... | 2015 | 25713141 |
| a reliable liquid chromatography-tandem mass spectrometry method for simultaneous determination of multiple mycotoxins in fresh fish and dried seafoods. | a reliable liquid chromatography-tandem mass spectrometry (lc-ms/ms) method was developed for simultaneous determination of nine mycotoxins, i.e., aflatoxin b1 (afb1), aflatoxin b2 (afb2), aflatoxin g1 (afg1), aflatoxin g2 (afg2), ochratoxin a (ota), zearalenone (zen), t-2 toxin, ht2 toxin and deoxynivalenol (don), in fresh fish (muscle and entrails) as well as dried seafoods. special focus was given to sample pretreatment which is crucial for an accurate and reliable analytical method. with reg ... | 2015 | 25711425 |
| mitochondrial genome sequencing and development of genetic markers for the detection of dna of invasive bighead and silver carp (hypophthalmichthys nobilis and h. molitrix) in environmental water samples from the united states. | invasive asian bighead and silver carp (hypophthalmichthys nobilis and h. molitrix) pose a substantial threat to north american aquatic ecosystems. recently, environmental dna (edna), genetic material shed by organisms into their environment that can be detected by non-invasive sampling strategies and genetic assays, has gained recognition as a tool for tracking the invasion front of these species toward the great lakes. the goal of this study was to develop new species-specific conventional pcr ... | 2015 | 25706532 |
| enhancement of secondary metabolites from bacillus licheniformis xy-52 on immune response and expression of some immune-related genes in common carp, cyprinus carpio. | this study was undertaken to isolate active secondary metabolites from immunostimulatory bacillus licheniformis xy-52 and evaluate their activities at 0%, 0.5%, 1.0%, and 2.0% doses supplementation with feed on immune response in common carp at weeks 1, 2, and 3. by applying chromatography techniques and successive recrystallization, two purified metabolites were obtained and identified by spectral data (mass spectrometry and nuclear magnetic resonance) as: cyclo-(phe-tyr) and cyclo-(phe-gly). t ... | 2015 | 25703710 |
| rational development of an attenuated recombinant cyprinid herpesvirus 3 vaccine using prokaryotic mutagenesis and in vivo bioluminescent imaging. | cyprinid herpesvirus 3 (cyhv 3) is causing severe economic losses worldwide in common and koi carp industries, and a safe and efficacious attenuated vaccine compatible with mass vaccination is needed. we produced single deleted recombinants using prokaryotic mutagenesis. when producing a recombinant lacking open reading frame 134 (orf134), we unexpectedly obtained a clone with additional deletion of orf56 and orf57. this triple deleted recombinant replicated efficiently in vitro and expressed an ... | 2015 | 25700279 |
| [differences in the infestation rate of young cyprinid fishes (cypriniformes) by metacercaria of posthodiplostomum cuticola (digenea, diplostomatidae) in river and lake systems of the lake chany basin (western siberia)]. | a total of 12 fish species were studied for the invasion of p. cuticola (nordmann, 1832) metacercariae in the chany lake estuaries, river and lake systems during different periods of water level. all infected individuals were represented by juveniles of the family cyprinidae, except for adults of the dace leuciscus leuciscus. (l.). under an average water level (2010) the highest rate of fish invasion was revealed in the estuary zone, where the prevalence of infection (pi) constituted 37.5%, 13.4 ... | 2015 | 25693328 |
| bioconcentration and trophic transfer of polychlorinated biphenyls and polychlorinated dibenzo-p-dioxins and dibenzofurans in aquatic animals from an e-waste dismantling area in east china. | eight aquatic biota species were collected from an e-waste dismantling area in east china to investigate bioconcentration and trophic transfer of polychlorinated biphenyls (pcbs), polychlorinated dibenzo-p-dioxins (pcdds) and polychlorinated dibenzofurans (pcdfs). the mean concentrations of pcbs varied widely from 6.01 × 10(4) to 2.27 × 10(6) pg per g dry weight (dw). the ∑25pcb concentrations in eels were significantly higher than those in other species. the levels of pcdd/fs changed from 8.13 ... | 2015 | 25689167 |
| ulcer disease prophylaxis in koi carp by bath immersion with chicken egg yolk containing anti-aeromonas salmonicida igy. | ulcer disease, caused by atypical aeromonas salmonicida, is a serious concern in ornamental koi carp, because it induces skin ulceration, disfiguring ornamental fish and causing economic loses. the present study aimed to establish a novel prophylaxis with chicken egg yolk immunoglobulin, igy, against ulcer disease and to assess its feasibility in the ornamental fish industry. addition of egg yolk powder containing anti-a. salmonicida igy to rearing water provided significant protection against a ... | 2015 | 25687817 |
| immune-relevant thrombocytes of common carp undergo parasite-induced nitric oxide-mediated apoptosis. | common carp thrombocytes account for 30-40% of peripheral blood leukocytes and are abundant in the healthy animals' spleen, the thrombopoietic organ. we show that, ex vivo, thrombocytes from healthy carp express a large number of immune-relevant genes, among which several cytokines and toll-like receptors, clearly pointing at immune functions of carp thrombocytes. few studies have described the role of fish thrombocytes during infection. carp are natural host to two different but related protozo ... | 2015 | 25681740 |
| effect of dietary glutamine on growth performance, non-specific immunity, expression of cytokine genes, phosphorylation of target of rapamycin (tor), and anti-oxidative system in spleen and head kidney of jian carp (cyprinus carpio var. jian). | this study was designed to investigate the effects of dietary glutamine on the growth performance, cytokines, target of rapamycin (tor), and antioxidant-related parameters in the spleen and head kidney of juvenile jian carp (cyprinus carpio var. jian). fish were fed the basal (control) and glutamine-supplemented (12.0 g glutamine kg(-1) diet) diets for 6 weeks. results indicated that the dietary glutamine supplementation improved the growth performance, spleen protein content, serum complement 3 ... | 2015 | 25675866 |
| rapid genomic dna changes in allotetraploid fish hybrids. | rapid genomic change has been demonstrated in several allopolyploid plant systems; however, few studies focused on animals. we addressed this issue using an allotetraploid lineage (4nat) of freshwater fish originally derived from the interspecific hybridization of red crucian carp (carassius auratus red var., ♀, 2n=100) × common carp (cyprinus carpio l., ♂, 2n=100). we constructed a bacterial artificial chromosome (bac) library from allotetraploid hybrids in the 20th generation (f20) and sequenc ... | 2015 | 25669608 |
| identification and functional characterization of the novel edwardsiella tarda effector esej. | edwardsiella tarda is a gram-negative enteric pathogen that causes hemorrhagic septicemia in fish and gastro- and extraintestinal infections in humans. the type iii secretion system (t3ss) of e. tarda has been identified as a key virulence factor that contributes to pathogenesis in fish. however, little is known about the associated effectors translocated by this t3ss. in this study, by comparing the profile of secreted proteins of the wild-type ppd130/91 and its t3ss atpase δesan mutant, we ide ... | 2015 | 25667268 |
| effect of cholesterol-loaded cyclodextrin on cryosurvival and fertility of cryopreserved carp (cyprinus carpio) sperm. | addition of cholesterol-loaded cyclodextrin (clc) to the diluents of mammalian semen increased stability and rigidity of phospholipid hydrocarbon chains of plasma membrane during sperm cryopreservation process. clc has been tested successfully as cryoprotectant in various livestock sperm cryopreservation protocols but its efficacy for cryopreserving of fish sperm has not previously been tested. in the present study, different cholesterol loaded cyclodextrin concentrations were evaluated for the ... | 2015 | 25661710 |
| morphological polymorphism in tapeworms: redescription of caryophyllaeus laticeps (pallas, 1781) (cestoda: caryophyllidea) and characterisation of its morphotypes from different fish hosts. | recent morphological and molecular data have shown that one of the most common parasites of freshwater fish in the palaearctic region, the cestode caryophyllaeus laticeps (pallas, 1781) (eucestoda: caryophyllidea), is highly polymorphic. five distinct morphotypes of c. laticeps, largely corresponding to different fish hosts and representing separate, yet closely related genetic lineages, have been recognised and they are characterised in the present paper. morphotype 1 from breams, abramis brama ... | 2015 | 25655116 |
| first record of the invasive asian fish tapeworm bothriocephalus acheilognathi in honduras, central america. | this paper provides the first report of the invasive asian fish tapeworm, bothriocephalus acheilognathi yamaguti, 1934, in honduras. the cestode was found in profundulus portillorum (cyprinodontiformes: profundulidae), which represents a new host record, and which is a member of a genus faced with a variety of conservation challenges, now potentially complicated by the presence of this pathogenic cestode. nearly complete sequence data from the its-1 5.8s and its-2 regions corroborate the determi ... | 2015 | 25654444 |
| immunomodulatory effect of arsenic on cytokine and hsp gene expression in labeo rohita fingerlings. | immune system is fundamental for survival of an organism against invading pathogens and other harmful agents. cytokines, the signaling proteins that are produced transiently after cell activation and exert pleiotropic effects on cells of the immune system, are important mediators of cell mediated immune response. when expressed in a dysregulated fashion cytokines can underlie either immunodeficient or immunopathologic states. heat shock proteins (stress proteins, hsps) are also key proteins, whi ... | 2015 | 25652292 |