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change in the biotype composition of bemisia tabaci in shandong province of china from 2005 to 2008.certain biotypes of the bemisia tabaci (gennadius) (hemiptera: aleyrodidae) complex cause extensive damage and are important pests and virus vectors in agricultural crops throughout the world. among the most invasive and well studied are the b and q biotypes. recent reports in shandong province, china, have indicated that the q biotype was introduced there in approximately 2005, whereas the b biotype has been established there for approximately 10 yr. even so, the present distribution of the two ...201020550819
effects of food deprivation on host feeding and parasitism of whitefly parasitoids.most whitefly parasitoids kill whiteflies in one of two ways, by piercing the whitefly body with the ovipositor and laying an egg or by piercing the body and feeding on host fluids. the effects of food deprivation on host-feeding and parasitizing capacity of the whitefly parasitoids eretmocerus melanoscutus and encarsia formosa (hymenoptera: aphelinidae) were determined in this study. we investigated the effects of various food-deprivation durations (0, 3, 6, 10, and 24 h, and 20% honey solution ...201020550806
induced defense by bemisia tabaci biotype b (hemiptera: aleyrodidae) in tobacco against myzus persicae (hemiptera: aphididae).myzus persicae and bemisia tabaci are serious pests of tobacco and can occur simultaneously on the same plant. we found that tobacco plants infested by whiteflies had fewer aphids than those without whiteflies. to determine whether b. tabaci feeding could induce plant defense against aphids locally and systemically, we determined the effects of b. tabaci on several biological parameters of m. persicae on tobacco. infestation of b. tabaci nymphs reduced survival rates of m. persicae by 30.0%. in ...201020550802
legume yellow mosaic viruses: genetically isolated begomoviruses.summary: the yellow mosaic diseases of a number of legumes across southern asia are caused by four species of whitefly-transmitted geminiviruses (genus begomovirus, family geminiviridae): mungbean yellow mosaic virus, mungbean yellow mosaic india virus, dolichos yellow mosaic virus and horsegram yellow mosaic virus. they cause losses to a number of important pulse crops, a major source of dietary protein in the region. the viruses have host ranges limited to plants of the family fabaceae and eff ...200720507504
name rectification and comparisons between nephaspis cocois gordon and stethorus minutalus gordon & chapin (coleoptera: coccinellidae).this note has the purpose to rectify the identification of stethorus minutalus gordon & chapin as a predator of bemisia tabaci gennadius (hemiptera: aleyrodidae), for its correct name, nephaspis cocois gordon in a recent published paper. similarities and differences between these two species are presented to avoid future misidentifications.201020498975
[transmission of tomato venezuela virus by bemisia tabaci (gennadius) (hemiptera: aleyrodidae), in maracaibo, venezuela].the biological transmission of tomato venezuela virus (tovev) by biotype b of the whitefly species bemisia tabaci (gennadius) increased (21.7-95.0%), and the time for symptom expression decreased (16-12.6 days) as the number of viruliferous whiteflies allowed access for inoculation to susceptible tomato plants increased from 1 to 20 adults/plant. when acquired only as a nymph, adults of this biotype transmitted the virus to 88.3% of susceptible tomato plants, using 15 viruliferous individuals pe ...201020498966
[molecular characterization and genetic divergence of bemisia tabaci (genn.) (hemiptera: aleyrodidae) on different crops and growing areas].knowledge on the genetic variation of populations of bemisia tabaci (genn.) can improve the understanding of genetic diversity found in their biotypes and, consequently, offer guidelines for its management. in this study, the molecular characterization was performed and genetic diversity data were obtained for this insect from three regions of brazil on different crops [cotton and soybean (mato grosso - mt); cabbage (distrito federal - df); soybean and potato (são paulo - sp)], using rapd marker ...201020498959
differential gene expression in whitefly (bemisia tabaci) b-biotype females and males under heat-shock condition.bemisia tabaci (insecta, hemiptera, aleyrodidae) females are more heat resistant than males, which has important ecological significance in adaptation and expansion of b. tabaci populations. differentially expressed genes between 25 degrees c and 44 degrees c were identified by suppression subtractive hybridization (ssh) in b. tabaci sexes. 50 and 83 differentially expressed expression sequence tags (ests) were obtained from female and male libraries, respectively. the ests have four functional ...200820494845
host range and complete genome sequence of cucurbit chlorotic yellows virus, a new member of the genus crinivirus.cucurbit chlorotic yellows virus (ccyv) causes chlorotic yellows on cucumber (cucumis sativus) and melon (cucumis melo) and is transmitted by bemisia tabaci biotype b and q whiteflies. to characterize the host range of ccyv, 21 cucurbitaceous and 12 other plant species were inoculated using whitefly vectors. all tested cucumis spp. except cucumis anguria and cucumis zeyheri were systemically infected with ccyv, although infection rates varied among species. citrullus lanatus, cucurbita pepo, and ...201020465411
co-infection and localization of secondary symbionts in two whitefly species.whiteflies are cosmopolitan phloem-feeding pests that cause serious damage to many crops worldwide due to direct feeding and vectoring of many plant viruses. the sweetpotato whitefly bemisia tabaci (gennadius) and the greenhouse whitefly trialeurodes vaporariorum (westwood) are two of the most widespread and damaging whitefly species. to complete their unbalanced diet, whiteflies harbor the obligatory bacterium portiera aleyrodidarum. b. tabaci further harbors a diverse array of secondary symbio ...201020462452
life table analysis for immatures and female adults of the predatory beetle, delphastus catalinae, feeding on whiteflies under three constant temperatures.immature development and reproductive life history of delphastus catalinae (horn) (coleoptera: coccinellidae) feeding on bemisia tabaci biotype b (gennadius) (homoptera: aleyrodidae) (= b. argentifolii bellows and perring) immatures was studied at three constant temperatures: 22, 26 and 30 degrees c. lower developmental threshold temperatures (t0) were estimated at 9 and 9.9 degrees c, for males and females, respectively. female adults weighed slightly more than males (0.587 and 0.505 mg, respec ...200820345295
critical feeding periods for last instar nymphal and pharate adults of the whiteflies, trialeurodes vaporariorum and bemisia tabaci.a critical feeding period is the time after which 50% of a given species of insect can be removed from its food source and complete development by undergoing adult eclosion. the critical feeding period was determined for the greenhouse white fly, trialeurodes vaporariorum, and the sweet potato whitefly, bemisia tabaci (biotype b) (homptera/hemiptera: aleyrodidae). fourth (last) instar and pharate adult whiteflies were removed from green bean leaves, staged, placed on filter paper in small petri ...200720302459
biological agents for whitefly control in sardinian greenhouse tomatoes.to evaluate the effectiveness of alternative options for biocontrol of whiteflies in greenhouse tomatoes, an experiment was carried out during the cropping season 2005-2006 in one of sardinia's major horticultural districts (s. margherita di pula, cagliari, italy). twelve long-cycle and 17 short-cycle tomato crops (8 autumn and 9 spring crops) were surveyed. all of them were treated for insect pest control at the beginning of the growing season, but in 19 out of 29 cases whitefly natural enemies ...200920222592
seasonal trends of whitefly populations in a mediterranean tomato growing area.the whiteflies bemisia tabaci and trialeurodes vaporariorum and their associated viruses constitute a major threat to tomato crops in the mediterranean region. continuous host availability and mild climate are thought to be among the factors contributing to the outbreaks of whitefly-related problems in this area. we carried out a year-long survey to investigate the relative contribution of different plants, agricultural and not, and indoor/outdoor crops as hosts of the two whiteflies and the tom ...200920222589
severe outbreak of tomato yellow leaf curl sardinia virus on pepper in southern italy.during summer and autumn 2008 a severe outbreak of pepper leaf curl disease (plcd) was observed in pepper crops under plastic tunnels in the ionic coast of basilicata region. its incidence reached, in some cases, values close to 50%. the beginning of infections was recorded along the perimeter of the tunnels, where it reached a percentage of almost 100%. the infection then progressively spread towards the central areas of the greenhouses. large populations of whiteflies, identificated as bemisia ...200920222578
an epidemiological survey of tylcd in southern sardinia (italy).tomato yellow leaf curl sardinia virus (tylcsv) and tomato yellow leaf curl virus (tylcv) are among the most virulent pathogens of greenhouse tomatoes in sardinia (italy). to investigate the relationship between seasonal population trends of the vector bemisia tabaci and the dynamics of tylcd spread to susceptible crops, between may and october 2007 we carried out a survey in a tomato growing area located in the south of the island. on three farms specialized in the production of fresh market to ...200920222569
integrated approach to tylcd management in sardinia (italy).in sardinia (italy) tomato yellow leaf curl disease (tylcd) re-emerged in 2003, after a period of decline, producing severe yield losses in protected tomato crops. this epidemic outbreak highlighted the inadequacy of the approach to tylcd management based chiefly on the chemical control of its vector, the whitefly bemisia tabaci, and the use of 40-mesh nets for greenhouse screening. to evaluate the reliability of alternative practices for implementation within ip&dm programmes, in 2007 and 2008 ...200920222567
prevalence of endosymbionts in bemisia tabaci populations and their in vivo sensitivity to antibiotics.bemisia tabaci can harbor both primary and secondary endosymbionts, and the specific endosymbionts can differ among different b. tabaci biotypes. this study determined (1) the prevalence of the primary endosymbiont portiera aleyrodidarum and secondary endosymbionts arsenophonus and wolbachia in two invasive biotypes (b and q) and one indigenous biotype (cv) in china and (2) the in vivo effect of three antibiotics (tetracycline, ampicillin trihydrate, and rifampicin) against the endosymbionts; if ...201020217091
pyriproxyfen resistance of bemisia tabaci (homoptera: aleyrodidae) biotype b: metabolic mechanism.juvenile hormone (jh) analog insecticides are relatively nontoxic to vertebrates and provide efficient control of key arthropod pests. one jh analog, pyriproxyfen, has provided over a decade of exceptional management of whiteflies in cotton of the southwestern united states. thwarting resistance to pyriproxyfen in bemisia tabaci (gannadius) (a.k.a. bemisia argentifolii bellows and perring) has been the focus of an integrated resistance management program because this insecticide was first regist ...201020214381
mapping and validation of qtls for resistance to aphids and whiteflies in melon.aphis gossypii and bemisia tabaci are severe hemipteran pests of melon crops and breeding for resistance to both insects is required to reduce pesticide use. resistance was evaluated for its effect on behaviour and biotic potential of both hemipterans in a population of recombinant inbred lines (rils) derived from the cross védrantais x pi 161375. insect variability was considered using two a. gossypii clones and two b. tabaci populations. two additive qtls affected the whiteflies. four additive ...201020180095
reproductive incompatibility among genetic groups of bemisia tabaci supports the proposition that the whitefly is a cryptic species complex.the worldwide distribution and extensive genetic diversity of the whitefly bemisia tabaci has long been recognized. however, whether b. tabaci is a complex species or a species complex has been a subject of debate. recent phylogenetic analyses suggest that b. tabaci is a cryptic species complex composed of at least 24 morphologically indistinguishable species. here, we conducted crossing experiments and demonstrated reproductive incompatibility among three of the 24 putative species. our data an ...201020178675
mating behaviour, life history and adaptation to insecticides determine species exclusion between whiteflies.1. negative interspecific interactions, such as resource competition or reproductive interference, can lead to the displacement of species (species exclusion). 2. here, we investigated the effect of life history, mating behaviour and adaptation to insecticides on species exclusion between cryptic whitefly species that make up the bemisia tabaci species complex. we conducted population cage experiments independently in china, australia, the united states and israel to observe patterns of species ...201020163491
evidence for pre-zygotic reproductive barrier between the b and q biotypes of bemisia tabaci (hemiptera: aleyrodidae).the degree of reproductive isolation between the b and q biotypes of the whitefly bemisia tabaci (hemiptera: aleyrodidae) is currently not clear. laboratory experiments have shown that the two biotypes are capable of producing viable f1 hybrids but that these females are sterile as their f2 generation failed to develop, indicating, most likely, a post-zygotic reproductive barrier. here, we confirm, by molecular and ecological tools, that the b and q biotypes of israel are genetically isolated an ...201020158928
differentiation of tomato yellow leaf curl virus and tomato yellow leaf curl sardinia virus using real-time taqman pcr.during the past four decades, tomato yellow leaf curl disease has become one of the major constraints in tomato production worldwide. in the mediterranean basin, several isolates from two major begomovirus species are involved in outbreaks and persistent epidemics. a real-time taqman pcr assay was developed and evaluated for the rapid and multiplex detection and differentiation of two begomoviruses often found in mixed infections in the region, tomato yellow leaf curl virus (tylcv) and tomato ye ...201020153376
[genetic variability of the bemisia tabaci (gennadius) biotype b (hemiptera: aleyrodidae) in vegetable crops in são luís, state of maranhão, brazil].the rapd technique is widely used to investigate the distinct genetic characteristics of the complex bemisia tabaci (gennadius), which is currently constituted of approximately 41 biotypes. the objective of this research was to characterize populations of whitefly collected in crops of agricultural producing areas in são luís, ma, like okra, beans and pepper, using rapd molecular markers. females from nine whitefly populations were analyzed and compared with b. tabaci biotype b taken from poinse ...200920098922
[phagodeterrent activity of the plants tithonia diversifolia and montanoa hibiscifolia (asteraceae) on adults of the pest insect bemisia tabaci (homoptera: aleyrodidae)].bemisia tabaci (gennadius) is a polyphagous, cosmopolitan and worldwide relevant pest, mainly acting as a virus vector on many crops. a sound preventive approach to deal with it would be the application of repellent or deterrent substances hopefully present in tropical plants, which in turn may contribute to take advantage of the remarkable rich mesoamerican biodiversity. therefore, extracts of two wild plants belonging to family asteraceae, titonia (tithonia diversifolia) and "tora" (montanoa h ...200920073345
effects of morphological characteristics of cucumis sativus seedlings grown at different vapor pressure deficits on initial colonization of bemisia tabaci (hemiptera: aleyrodidae).we investigated the effects of morphological characteristics of cucumber, cucumis sativus l., seedlings grown at different vapor pressure deficits (vpds) on initial colonization of whitefly bemisia tabaci (gennadius) (hemiptera: aleyrodidae) biotype b. the seedlings were grown at a vpd of 3.8 or 0.4 kpa for 9 d at 30 degrees c under a photoperiod of 12:12 (l:d) h. pairs of seedlings, one grown at high vpd and the other at low vpd, were then installed in cages with a vpd of 1.3 kpa, and adults of ...200920069856
viral infection of tobacco plants improves performance of bemisia tabaci but more so for an invasive than for an indigenous biotype of the whitefly.the ecological effects of plant-virus-vector interactions on invasion of alien plant viral vectors have been rarely investigated. we examined the transmission of tomato yellow leaf curl china virus (tylccnv) by the invasive q biotype and the indigenous zhj2 biotype of the whitefly bemisia tabaci, a plant viral vector, as well as the influence of tylccnv-infection of plants on the performance of the two whitefly biotypes. both whitefly biotypes were able to acquire viruses from infected plants an ...201020043350
inoculation of tomato plants (solanum lycopersicum) with growth-promoting bacillus subtilis retards whitefly bemisia tabaci development.root inoculation of tomato (solanum lycopersicum) plants with a bacillus subtilis strain beb-dn (bsdn) isolated from the rhizosphere of cultivated potato plants was able to promote growth and to generate an induced systemic resistance (isr) response against virus-free bemisia tabaci. growth promotion was evident 3 weeks after inoculation. no changes in oviposition density, preference and nymphal number in the early stages of b. tabaci development were observed between bsdn-treated plants and con ...201020041333
[effects of host plants on selection behavior and biological parameters of bemisia tabaci gennadius biotype b].based on the observation of free diffusion in petri dish and biology, the selection behavior and biological parameters of bemisia tabaci gennadius biotype b on host plants cucumber, eggplant, pepper, cotton, and sweet potato were investigated. no difference was observed in the individual number of b. tabaci on the five host plant leaves during the early period of free diffusion (within 2 h), but changes were found within the following periods (4-48 h), i.e., the b. tabaci individuals increased o ...200920030150
whiteflies interfere with indirect plant defense against spider mites in lima bean.plants under herbivore attack are able to initiate indirect defense by synthesizing and releasing complex blends of volatiles that attract natural enemies of the herbivore. however, little is known about how plants respond to infestation by multiple herbivores, particularly if these belong to different feeding guilds. here, we report the interference by a phloem-feeding insect, the whitefly bemisia tabaci, with indirect plant defenses induced by spider mites (tetranychus urticae) in lima bean (p ...200919965373
cross-resistance study and biochemical mechanisms of thiamethoxam resistance in b-biotype bemisia tabaci (hemiptera: aleyrodidae).b-biotype bemisia tabaci (gennadius) has invaded china over the past two decades. to understand the risks and to determine possible mechanisms of resistance to thiamethoxam in b. tabaci, a resistant strain was selected in the laboratory. cross-resistance and the biochemical mechanisms of thiamethoxam resistance were investigated in the present study.201019937914
fifty years of the integrated control concept: moving the model and implementation forward in arizona.fifty years ago, stern, smith, van den bosch and hagen outlined a simple but sophisticated idea of pest control predicated on the complementary action of chemical and biological control. this integrated control concept has since been a driving force and conceptual foundation for all integrated pest management (ipm) programs. the four basic elements include thresholds for determining the need for control, sampling to determine critical densities, understanding and conserving the biological contro ...200919834884
bemisia tabaci-infested tomato plants show a phase-specific pattern of photosynthetic gene expression indicative of disrupted electron flow leading to photo-oxidation and plant death.a suppression-subtractive-hybridization (ssh) strategy led to the identification of several genes whose expression was differentially modified in response to different larval phases present during the infestation process of tomato plants (solanum lycopersicum) by virus-free whitefly bemisia tabaci (bt). the findings regarding photosynthetic gene expression were in accordance to previous studies reporting altered patterns of expression as a result of insect herbivory. however, the examination, in ...200919826216
begomovirus coat protein interacts with a small heat-shock protein of its transmission vector (bemisia tabaci).tomato yellow leaf curl sardinia virus (tylcsv) is transmitted from plant to plant by the whitefly bemisia tabaci in a persistent-circulative manner. the coat protein (cp) plays an important role in this transmission cycle. in this study, the cp was used to screen a bemisia tabaci cdna library using the yeast two-hybrid system, in a search for interacting partners. a member of the small heat-shock protein family (termed bthsp16) was identified and its interaction with the cp was verified by an i ...200919817909
[biological characters of bemisia tabaci (gennadius) experimental population on cotton varieties in xinjiang].the development, survival, reproduction, and life-span of bemisia tabaci (gennadius) on six major cotton varieties (xinluzao 8, xinluzao 16, xinluzao 17, zhongmian 35, kang 5, and dai 80) in xinjiang were studied under the conditions of (26 +/- 1) degrees c, relative humidity (75 +/- 1)%, and illumination l:d = 16:8 in laboratory. the life table of the b. tabaci experimental population was established, and the population net reproductive rate (r0), intrinsic rate of increase (rm), mean generatio ...200919795661
monitoring neonicotinoid resistance in biotype b of bemisia tabaci in florida.biotype b of the sweetpotato whitefly, bemisia tabaci (genn.), is a worldwide pest that has developed resistance to many insecticides, including the neonicotinoid class. florida field populations were monitored for susceptibility to the neonicotinoids imidacloprid and thiamethoxam using a cut leaf petiole bioassay method.201019790225
[resistance of squash genotypes to bemisia tabaci (genn.) biotype b (hemiptera: aleyrodidae)].field assays were performed to evaluate the attractiveness and the non-preference of whitefly bemisia tabaci (genn.) biotype b for oviposition on squash genotypes (cucurbita pepo) and to observe the susceptibility of genotypes (novita, sandy, caserta cac melhorada, novita plus, samira, bianca, af-2858 and caserta ts) to silverleaf symptoms. the sandy genotype was the least attractive to whitefly, while novita plus, af-2858 and samira were the most attractive. the caserta cac melhorada genotype w ...200919768274
effect of commercially available plant-derived essential oil products on arthropod pests.plant-derived essential oil products, in general, are considered minimum-risk pesticides and are exempt from environmental protection agency registration under section 25(b) of the federal insecticide fungicide and rodenticide act. however, many of the plant-derived essential products available to consumers (homeowners) have not been judiciously evaluated for both efficacy and plant safety. in fact, numerous plant-derived essential oil products labeled for control of arthropod pests have not bee ...200919736770
laboratory evaluation of products to reduce settling of sweetpotato whitefly adults.the impact of trademarked and commercial products on settling of adults of the sweetpotato whitefly, bemisia tabaci (gennadius), was studied in the laboratory. a no-choice bioassay using leaf disks of tomato, solanum esculentum l., was developed to evaluate the impact of concentration series of products on settling of b. tabaci adults. the concentration of each product that would reduce settling by 50% (sc50) was estimated for each product using standard probit analyses, and the values were comp ...200919736760
structural model and functional characterization of the bemisia tabaci cyp6cm1vq, a cytochrome p450 associated with high levels of imidacloprid resistance.the neonicotinoid imidacloprid is one of the most important insecticides worldwide. it is used extensively against the whitefly bemisia tabaci (hemiptera: aleyrodidae), an insect pest of eminent importance globally, which was also the first pest to develop high levels of resistance against imidacloprid and other neonicotinoids in the field. recent reports indicated that in both the b and q biotypes of b. tabaci, the resistant phenotype is associated with over-expression of the cytochrome p450 ge ...200919716416
the role of specific tomato volatiles in tomato-whitefly interaction.bemisia tabaci (whitefly) infestations and the subsequent transfer of viruses are the cause of severe losses in crop production and horticultural practice. to improve biological control of b. tabaci, we investigated repellent properties of plant-produced semiochemicals. the mix of headspace volatiles, collected from naturally repellent wild tomato accessions, influenced b. tabaci initial choice behavior, indicating a role for plant semiochemicals in locating host plants. a collection of wild tom ...200919692533
thermotolerance and gene expression following heat stress in the whitefly bemisia tabaci b and q biotypes.the whitefly bemisia tabaci (gennadius) causes tremendous losses to agriculture by direct feeding on plants and by vectoring several families of plant viruses. the b. tabaci species complex comprises over 10 genetic groups (biotypes) that are well defined by dna markers and biological characteristics. b and q are amongst the most dominant and damaging biotypes, differing considerably in fecundity, host range, insecticide resistance, virus vectoriality, and the symbiotic bacteria they harbor. we ...200919683053
[effects of alcohol extracts from three kinds of biomass energy plant tissues on biological activity of bemisia tabaci].to test the feasibility of using raw extracts from the tissues of biomass energy plants ricinus communi and kosteletzkya virginica as plant protection agents, the alcohol extracts from r. communi seed and leaf and from k. virginica leaf were used to treat adult bemisia tabaci by spraying. the glutathione s-transferase and carboxylesterase activities in b. tabaci body were measured after treated for 4 h, 24 h, 48 h, 72 h, and 96 h, and the olfaction responses of b. tabaci to the alcohol extracts ...200919637599
agroinoculation of the crinivirus, lettuce infectious yellows virus, for systemic plant infection.lettuce infectious yellows virus (liyv) is phloem-limited, non-mechanically transmissible, and is transmitted to plants only by bemisia tabaci. here, we developed agroinoculation to deliver liyv to plants thereby obviating the need for b. tabaci. agroinfiltration of rna 1 containing a green fluorescent protein gene into nicotiana benthamiana leaves resulted in subliminal infections, as judged by green fluorescence. agroinfiltration of liyv wild-type rna 1 and 2 constructs resulted in systemic in ...200919632699
differential gene expression in whitefly bemisia tabaci-infested tomato (solanum lycopersicum) plants at progressing developmental stages of the insect's life cycle.a suppression-subtractive-hybridization (ssh) strategy was used to identify genes whose expression was modified in response to virus-free whitefly bemisia tabaci (bt, biotype a) infestation in tomato (solanum lycopersicum) plants. thus, forward and reverse ssh gene libraries were generated at four points in the whitefly's life cycle, namely at (1) 2 days (adult feeding and oviposition: phase i); (2) 7 days (mobile crawler stage: phase ii); (3) 12 days (second to third instar nymphal transition: ...200919627556
aqueous extracts of some medicinal plants are as toxic as imidacloprid to the sweet potato whitefly, bemisia tabaci.aqueous extracts of nine plants, known to have medicinal activity, were tested for their toxicity against the sweet potato whitefly, bemisia tabaci genn. (homoptera: aleurodidae) compared to the toxicity of the insecticide, imidacloprid. extracts of lepidiuim sativum l. (brassicales: brassicaceae) killed 71 % of early stage nymphs, which was not significantly different from mortality caused by imidacloprid. treatment of pupae with three plant extracts, l. sativum, achillea biebersteinii l. (aste ...200919613450
oviposition, development and survivorship of the sweetpotato whitefly bemisia tabaci on soybean, glycine max, and the garden bean, phaseolus vulgaris.abstract oviposition, development and survivorship of bemisia tabaci (gennadius) (hemiptera: aleyrodidae) were evaluated on soybean and garden bean under laboratory conditions of 26.0 +/- 0.5 degrees c, 70 - 80% rh and a photoperiod of 14:10 (l:d). b. tabaci deposited more eggs and survivorship of nymphs was significantly greater in a choice-test on soybean, glycine max l. (merr.) (fabeles: fabaceae), compared to the garden bean, phaseolus vulgaris l. overall developmental time from egg to adult ...200919611218
cross-resistance, inheritance and biochemical mechanisms of imidacloprid resistance in b-biotype bemisia tabaci.the b-type bemisia tabaci (gennadius) has become established in many regions in china, and neonicotinoids are extensively used to control this pest. imidacloprid resistance in a laboratory-selected strain of b-type b. tabaci was characterised in order to provide the basis for recommending resistance management tactics.200919562662
susceptibility to insecticides in the q biotype of bemisia tabaci is correlated with bacterial symbiont densities.the presence of symbiotic microorganisms may influence an insect's ability to tolerate natural and artificial stress agents such as insecticides. the authors have previously shown that rickettsia in the b biotype of the whitefly bemisia tabaci (gennadius) (homoptera: aleyrodidae) increases this insect's susceptibility to several insecticidal compounds. this communication reports a comparison of the susceptibilities of three isofemale strains of the q biotype of b. tabaci harbouring different bac ...200919479746
hypersensitive response to aphis gossypii glover in melon genotypes carrying the vat gene.aphis gossypii glover causes direct and indirect damage to cucumis melo l. crops. to decrease the harmful effects of this pest, one of the most economically and environmentally acceptable options is to use genetically resistant melon varieties. to date, several sources of resistance carrying the vat gene are used in melon breeding programmes that aim to prevent a. gossypii colonization and the subsequent aphid virus transmission. the results suggest that the resistance conferred by this gene is ...200919474089
improved dna barcoding method for bemisia tabaci and related aleyrodidae: development of universal and bemisia tabaci biotype-specific mitochondrial cytochrome c oxidase i polymerase chain reaction primers.whiteflies, heteropterans in the family aleyrodidae, are globally distributed and severe agricultural pests. the mitochondrial cytochrome c oxidase i (mtcoi) sequence has been used extensively in whitefly phylogenetic comparisons and in biotype identification of the agriculturally important bemisia tabaci (gennadius) whitefly. because of the economic importance of several whitefly genera, and the invasive nature of the b and the q biotypes of bemisia tabaci, mtcoi sequence data are continually g ...200919449657
distribution of bemisia tabaci (hemiptera: aleyrodidae) biotypes in florida-investigating the q invasion.after the 2004 discovery of the bemisia tabaci (gennadius) (hemiptera: aleyrodidae) q biotype in the united states, there was an urgent need to determine its distribution. as part of a coordinated country-wide effort, an extensive survey of b. tabaci biotypes was conducted in florida, with the cooperation of growers and state and federal agencies, to monitor the introduction and distribution of both the b and q biotypes. the biotype status of submitted b. tabaci samples was determined by polymer ...200919449648
antifeedant activity of botanical crude extracts and their fractions on bemisia tabaci (homoptera: aleyrodidae) adults: iii. quassia amara (simaroubaceae).bemisia tabaci is a key virus vector on several crops worldwide. then, a preventive approach to deal with viral epidemics may be the deployment of repellents or phagodeterrents at earlier stages of plant development (critical period). thus, the crude extract and four fractions thereof (water, water: methanol, methanol, and diethyl ether) of bitterwood (quassia amara, simaroubaceae) were tested for phagodeterrence to b. tabaci adults under greenhouse conditions, on tomato plants, in costa rica. b ...200819419106
antifeedant activity of botanical crude extracts and their fractions on bemisia tabaci (homoptera: aleyrodidae) adults: ii. sechium pittieri (cucurbitaceae).bemisia tabaci is a key pest of vegetables and other crops worldwide, but it is a particularly serious problem in the tropics, due to its ability to transmit several types of viruses, especially begomoviruses (geminiviridae). therefore, a preventive approach to deal with viral epidemics may be the deployment of repellents or phagodeterrents at earlier stages of plant development (critical period). thus, the crude extract and four fractions thereof (water, water: methanol, methanol, and diethyl e ...200819419105
antifeedant activity of botanical crude extracts and their fractions on bemisia tabaci (homoptera: aleyrodidae) adults: i. gliricidia sepium (fabaceae).bemisia tabaci is an important virus vector on a number of crops worldwide. therefore, a preventive approach to deal with viral epidemics may be the deployment of repellents or phagodeterrents at earlier stages of plant development (critical period). thus, the crude extract and four fractions thereof (water, water:methanol, methanol, and diethyl ether) of mother-of-cocoa (gliricidia sepium, fabaceae) were tested for phagodeterrence to b. tabaci adults under greenhouse conditions, on tomato plant ...200819419104
insecticide resistance status of bemisia tabaci q-biotype in south-eastern spain.bemisia tabaci gennadius q-biotype has readily developed resistance to numerous insecticide classes. studies in the mediterranean area are needed to clarify the resistance status and cross-resistance patterns in this invasive whitefly biotype. the levels of resistance in nymphs of seven strains of b. tabaci q-biotype from south-eastern spain to representative insecticides were determined.200919418483
synergistic interaction between the potyvirus, turnip mosaic virus and the crinivirus, lettuce infectious yellows virus in plants and protoplasts.lettuce infectious yellows virus (liyv), the type member of the genus crinivirus in the family closteroviridae, is specifically transmitted by the sweet potato whitefly (bemisia tabaci) in a semipersistent manner. liyv infections result in a low virus titer in plants and protoplasts, impeding reverse genetic efforts to analyze liyv gene/protein functions. we found that synergistic interactions occurred in mixed infections of liyv and turnip mosaic virus (tumv) in nicotiana benthamiana plants, an ...200919409943
a simple, rapid and inexpensive method for localization of tomato yellow leaf curl virus and potato leafroll virus in plant and insect vectors.a simple, rapid, inexpensive method for the localization of virus transcripts in plant and insect vector tissues is reported here. the method based on fluorescent in situ hybridization using short dna oligonucleotides complementary to an rna segment representing a virus transcript in the infected plant or insect vector. the dna probe harbors a fluorescent molecule at its 5' or 3' ends. the protocol: simple fixation, hybridization, minimal washing and confocal microscopy, provides a highly specif ...200919406154
identification of a mutation in the para-sodium channel gene of the cattle tick rhipicephalus (boophilus) microplus associated with resistance to synthetic pyrethroid acaricides.resistance against synthetic pyrethroid (sp) products for the control of cattle ticks in australia was detected in the field in 1984, within a very short time of commercial introduction. we have identified a mutation in the domain ii s4-5 linker of the para-sodium channel that is associated with resistance to sps in the cattle tick rhipicephalus (boophilus) microplus from australia. the cytosine to adenine mutation at position 190 in the r. microplus sequence af134216, results in an amino acid s ...200919367831
risk of damage to tomato crops by the generalist zoophytophagous predator nesidiocoris tenuis (reuter) (hemiptera: miridae).nesidiocoris tenuis (reuter) (hem. miridae) is a native zoophytophagous predator of the mediterranean region, and its populations colonize tomato crops when they are not heavily treated with insecticides. this generalist predator has a high capacity for controlling insect pests, and it is currently commercially produced and released in some areas to control bemisia tabaci (gennadius) (hem. aleyrodidae). however, its status as a pest and/or as beneficial is controversial. therefore, the aim of th ...201019366476
[oviposition behavior of the silverleaf whitefly bemisia tabaci biotype b on tomato].the objective of this work was to evaluate the influence of the leaf surface, the insect geotropic behavior and the type of foliar trichome on bemisia tabaci (genn.) biotype b oviposition on tomato leaves. bemisia tabaci females were confined in acrylic boxes in which tomato leaflets were fixed at the bottom and top part of the boxes to allow insects to access the leaf surface to be tested (adaxial and/or abaxial) in both no-choice and free choice tests. oviposition was always higher when the le ...200919347106
[monitoring the susceptibility to insecticides in bemisia tabaci (gennadius) (hemiptera: aleyrodidae) populations from brazil].monitoring the susceptibility of a pest population to pesticides is essential for resistance management programs. the objective of this research was to evaluate the genetic variability in pesticide susceptibility in populations of bemisia tabaci (gennadius) collected from different brazilian agricultural regions through the use of two different tests. four whitefly populations, two from goiás state (go-1 and go-2) e two from bahia state (ba-1 and ba-2), were tested against a susceptible referenc ...200919347105
virion stability is important for the circulative transmission of tomato yellow leaf curl sardinia virus by bemisia tabaci, but virion access to salivary glands does not guarantee transmissibility.the capsid protein (cp) of the monopartite begomovirus tomato yellow leaf curl sardinia virus (tylcsv), family geminiviridae, is indispensable for plant infection and vector transmission. a region between amino acids 129 and 152 is critical for virion assembly and insect transmissibility. two previously described mutants, one with a double q129p q134h mutation (pnhd) and another with a further d152e change (pnhe), were found nontransmissible (nt). another nt mutant with a single n130d change (qd ...200919321611
aerial electrostatic-charged sprays for deposition and efficacy against sweet potato whitefly (bemisia tabaci) on cotton.the efficacy of aerial electrostatic-charged sprays was evaluated for spray deposit characteristics and season-long control of sweet potato whitefly (swf), bemisia tabaci genn. biotype b (aka b. argentifolii bellows & perring), in an irrigated 24 ha cotton field. treatments included electrostatic-charged sprays at full and half active ingredient (ai) label rate, uncharged sprays and conventional sprays applied with cp nozzles at full label rate with several different insecticides.200919291696
molecular detection and identification of rickettsia endosymbiont in different biotypes of bemisia tabaci. 200919281454
almost there: transmission routes of bacterial symbionts between trophic levels.many intracellular microbial symbionts of arthropods are strictly vertically transmitted and manipulate their host's reproduction in ways that enhance their own transmission. rare horizontal transmission events are nonetheless necessary for symbiont spread to novel host lineages. horizontal transmission has been mostly inferred from phylogenetic studies but the mechanisms of spread are still largely a mystery. here, we investigated transmission of two distantly related bacterial symbionts--ricke ...200919274091
first record of tomato yellow leaf curl sardinia virus (tylcsv) on pepper in italy.during a survey in summer 2007, a disease of pepper (capsicum annuum) under plastic tunnels was observed in policoro (matera), on the ionic coast of basilicata region, with a disease incidence in some cases of more than 50%. affected cultivars were eppo and almund (s et g). the diseased plants exhibited light mosaic or mottling, leaf distortion, interveinal and marginal leaf chlorosis, upward curling of leaf margins of older leaves. the causal pathogen was suspected to be a begomovirus due to th ...200819226766
a real-time pcr assay to differentiate the b and q biotypes of the bemisia tabaci complex in cyprus.a real-time pcr assay based on taqman technology was developed and evaluated for the rapid detection of the b and q biotypes of bemisia tabaci (gennadius) (hemiptera: aleyrodidae). a survey was conducted during 2005-2007 in order to identify the distribution and prevalence of b. tabaci biotypes in cyprus using the real-time pcr assay. more than 700 adult whiteflies collected from 35 cultivated and weed plant species were individually haplotyped using taqman pcr, and the results of the assay were ...200919203404
first report and differential colonization of passiflora species by the b biotype of bemisia tabaci (gennadius) (hemiptera: aleyrodidae) in brazil.this note is the first report of bemisia tabaci (gennadius) biotype b colonizing passionvine in brazil. we examined the colonization of nine passiflora species by a wild b type population under greenhouse conditions. p. amethystina mikan was the most preferred species for oviposition and colonization, whereas p. suberosa l., p. coriacea juss. and two commercially cultivated species, p. alata curtis and p. edulis sims f. flavicarpa degener, were mostly uncolonised. p. morifolia mast., p. cincinna ...200819169568
effects of operational and environmental factors on evolution of resistance to pyriproxyfen in the sweetpotato whitefly (hemiptera: aleyrodidae).pyriproxyfen has been an important insecticide used as part of an integrated pest management (ipm) program for the sweetpotato whitefly, bemisia tabaci (gennadius) (b biotype), in arizona cotton. we used a simulation model to examine the effects of pyriproxyfen concentration, insecticide action thresholds, crop diversity, planting date, and pyriproxyfen decay on evolution of resistance to pyriproxyfen in b. tabaci. in the model, pyriproxyfen use was restricted to cotton with a limit of one appli ...200819161695
analyzing haplodiploid inheritance of insecticide resistance in whitefly biotypes.we developed new methods for analyzing inheritance of insecticide resistance in haplodiploid arthropods and applied them to elucidate resistance of the whitefly bemisia tabaci (gennadius) to an insect growth regulator, pyriproxyfen. two invasive biotypes of this devastating crop pest, the b biotype in arizona and the q biotype in israel, have evolved resistance to pyriproxyfen. here, we incorporated data from laboratory bioassays and crossing procedures exploiting haplodiploidy into statistical ...200919159498
newer insecticides for plant virus disease management.effective management of insect and mite vectors of plant pathogens is of crucial importance to minimize vector-borne diseases in crops. pesticides play an important role in managing vector populations by reducing the number of individuals that can acquire and transmit a virus, thereby potentially lowering disease incidence. certain insecticides exhibit properties other than lethal toxicity that affect feeding behaviours or otherwise interfere with virus transmission. to evaluate the potential of ...200919152818
invasive mechanism and management strategy of bemisia tabaci (gennadius) biotype b: progress report of 973 program on invasive alien species in china.bemisia tabaci (gennadius) biotype b, called a "superbug", is one of the most harmful biotypes of this species complex worldwide. in this report, the invasive mechanism and management of b. tabaci biotype b, based on our 5-year studies, are presented. six b. tabaci biotypes, b, q, zhj1, zhj2, zhj3 and fj1, have been identified in china. biotype b dominates the other biotypes in many regions of the country. genetic diversity in biotype b might be induced by host plant, geographical conditions, an ...200919152088
effects of temperature and culture media on vegetative growth of an entomopathogenic fungus isaria sp. (hypocreales: clavicipitaceae) naturally affecting the whitefly, bemisia tabaci in texas.the effects of temperature and mycological media on mycelial growth and estimates of spore production of an indigenous entomopathogenic fungus, isaria sp., found during natural epizootics on whiteflies in the lower rio grande valley of texas, were investigated. the radial growth (mm/day) of isaria sp. as a function of temperature fits a linear model; with faster growth on sabouraud dextrose agar with yeast extract, sday slopes (0.23) than on sabouraud maltose agar, sma slopes (0.14) from 20 to 3 ...200919125352
current status of insecticide resistance in q biotype bemisia tabaci populations from crete.a major problem of crop protection in crete, greece, is the control of bemisia tabaci (gennadius) (homoptera: aleyrodidae) with chemical insecticides owing to the rapid development of resistance. the aim of this study was to investigate the establishment of resistance and the underlying mechanisms to major insecticide classes with classical bioassays and known biochemical resistance markers.200919115232
lack of fitness costs associated with pyriproxyfen resistance in the b biotype of bemisia tabaci.the insect growth regulator pyriproxyfen has provided effective control of the whitefly bemisia tabaci gennadius in many countries. here, whether or not fitness costs were associated with pyriproxyfen resistance in a laboratory-selected resistant strain (qc02-r) of the b biotype was determined.200919097024
thermal effect on the biology and life tables of bemisia tabaci gennadius (homoptera: aleyrodidae).the life history and life table of bemisia tabaci gennadius on cucumber was studied under laboratory conditions at 20, 25 and 30 degrees c. the overall developmental time varied from 34.8 days at 20 degrees c to 14.1 days at 30 degrees c. immature mortality decreased from 45.8 to 17.3% with increasing temperature. the threshold temperatures of egg, 1st, 2nd, 3rd and 4th nymphal stage and a generation were 14.72, 14.36, 10.18, 11.40, 14.36 and 13.07 degrees c whereas the degree-day requirement at ...200719090279
some biological features of cotton whitefly, bemisia tabaci (homoptera: aleyrodidae) on various host plants.development and reproduction of the cotton whitefly, bemisia tabaci (gennadius) were studied on aubergine, tomato and potato under laboratory conditions (30 degrees c and 55% rh). total life cycle from egg to adult was 14.9, 20.0 and 14.2 days on aubergine, tomato and potato, respectively. immature mortality were 12.9, 18.1 and 12.3% at the same three host plants. females of b. tabaci oviposited means of 51.8, 60.1 and 67.5 eggs on aubergine, tomato and potato, respectively and had a mean longev ...200719090122
prey species preference of the predator serangium parcesetosum sicard (col., coccinellidae) and its interaction with another natural enemy.this study aimed to determine serangium parcesetosum preference for different prey species and parasitized bemisia tabaci (genn.) (hom., aleyrodidae) by the parasitoid, eretmocerus mundus mercet (hym., aphelinidae). the results on s. parcesetosum preference by feeding on different prey species offered separately and together indicated that the predatory larvae and adults preferred significantly the cotton whitefly, b. tabaci and the castor bean whitefly, trialeurodes ricini (misra) (hom., aleyro ...200719070175
inter-seasonal population dynamics and pest status of bemisia tabaci (gennadius) biotype b in an australian cropping system.bemisia tabaci, biotype b, commonly known as the silverleaf whitefly (slw) is an alien species that invaded australia in the mid-90s. this paper reports on the invasion ecology of slw and the factors that are likely to have contributed to the first outbreak of this major pest in an australian cotton cropping system. population dynamics of slw within whitefly-susceptible crop (cotton and cucurbit) and non-crop vegetation (sowthistle, sonchus spp.) components of the cropping system were investigat ...200919063758
biochemical and molecular analysis of deltamethrin resistance in the common bed bug (hemiptera: cimicidae).this study establishes deltamethrin resistance in a common bed bug, cimex lectularius l., population collected from new york city (ny-bb). the ny-bb population was 264-fold more resistant to 1% deltamethrin in contact bioassay compared with an insecticide-susceptible population collected in florida (fl-bb). general esterase, glutathione s-transferase, and 7-ethoxycoumarin o-deethylase activities of ny-bb were not statistically different from those of fl-bb. cdna fragments that encoded the open r ...200819058634
toxicities of destruxins against bemisia tabaci and its natural enemy, serangium japonicum.the bioactivities of destruxins against whitefly, bemisia tabaci and its natural enemy, ladybird beetle serangium japonicum were evaluated. destruxins a and b (da and db) showed insignificant ovicidal, oviposition deterrent and systemic insecticidal activities to b. tabaci; however, da and db had certain contact virulence to its nymphs. the lc(50) values of da at 120h to 2nd, 3rd and 4th instars were 89.8 (95% confidence interval as 85.4-94.4), 199.3 (187.7-211.5) and 270.7 (251.5-291.5)mg/l, wh ...200919000916
co-infection by two criniviruses alters accumulation of each virus in a host-specific manner and influences efficiency of virus transmission.tomato chlorosis virus (tocv), and tomato infectious chlorosis virus (ticv), family closteroviridae, genus crinivirus, cause interveinal chlorosis, leaf brittleness, and limited necrotic flecking or bronzing on tomato leaves. both viruses cause a decline in plant vigor and reduce fruit yield, and are emerging as serious production problems for field and greenhouse tomato growers in many parts of the world. the viruses have been found together in tomato, indicating that infection by one criniviru ...200819000010
post-release evaluation of eretmocerus hayati zolnerowich and rose in australia.bemisia tabaci biotype b is a significant pest of agriculture world-wide. it was first detected in australia in 1994. assessments of the potential of parasitoids already present in australia to control this pest indicated that two species of eretmocerus and 11 species of encarsia were present, but they did not exert sufficient control with a combined average of 5.0+/-0.3% apparent parasitism of 4th instars. further, only 25% of samples containing biotype b had parasitised individuals present. th ...200918947454
host plant influence on susceptibility of bemisia tabaci (hemiptera: aleyrodidae) to insecticides.a resistance monitoring program conducted for the polyphagous whitefly, bemisia tabaci (gennadius), in imperial valley, ca, usa generated a large set of lc50s for adults collected from broccoli, cantaloupe and cotton crops over a four-year period. a vial bioassay and, subsequently, a yellow-sticky card bioassay produced similar temporal profiles of relative susceptibilities to the pyrethroid insecticide bifenthrin. both bioassays revealed that whiteflies collected from broccoli were significantl ...200918947452
preinfestations of tomato plants by whiteflies (bemisia tabaci) or aphids (macrosiphum euphorbiae) induce variable resistance or susceptibility responses.in addition to constitutive plant resistance against pests or pathogens, plants can activate protective mechanisms upon contact with an invader or a chemical elicitor. studies on induced plant resistance to herbivores, especially piercing-sucking insects, are less abundant than those devoted to pathogens. several experiments under controlled conditions have been conducted to demonstrate that infestations by macrosiphum euphorbiae induce plant resistance to bemisia tabaci in susceptible tomato pl ...200918947449
quantitation over time of tomato yellow leaf curl geminivirus dna in its whitefly vector.abstract the amount of tomato yellow leaf curl geminivirus (tylcv) dna that accumulated in the vector bemisia tabaci was studied by quantitative chemiluminescent dot-blot assay, using digoxigenin-labeled specific dna probes. large groups of female whiteflies were allowed to feed for 4, 12, 24, or 48 h on tylcv-infected tomato plants and then were transferred to tylcv-immune cucumber plants. insects were sampled at different times during and after acquisition access and tested for tylcv-dna conte ...199718945078
sinaloa tomato leaf curl geminivirus: biological and molecular evidence for a new subgroup iii virus.abstract the biological and molecular properties of sinaloa tomato leaf curl virus (stlcv) were investigated in line with the hypothesis that stlcv is a previously uncharacterized, whitefly-transmitted geminivirus from north america. stlcv causes yellow leaf curl symptoms in tomato and yellow-green foliar mottle in pepper. five species belonging to two plant families were stlcv experimental hosts. stlcv had a persistent relationship with its whitefly vector, bemisia tabaci. polymerase chain reac ...199818944936
tomato chlorosis virus: a new whitefly-transmitted, phloem-limited, bipartite closterovirus of tomato.abstract tomato chlorosis virus (tocv) is the second whitefly-transmitted, phloem-limited, bipartite closterovirus described infecting tomato. tocv is distinct from tomato infectious chlorosis virus (ticv), based on lack of serological and nucleic acid cross-reactions and differences in vector specificity. ticv is transmitted only by the greenhouse whitefly (trialeurodes vaporariorum), whereas tocv is transmitted by the greenhouse whitefly, the banded-wing whitefly (t. abutilonea), and bemisia t ...199818944918
biotic, molecular, and phylogenetic characterization of bean calico mosaic virus, a distinct begomovirus species with affiliation in the squash leaf curl virus cluster.abstract bean calico mosaic virus (bcmov), a whitefly-transmitted geminivirus from sonora, mexico, was purified, and the genome components were cloned and sequenced. purified viral fractions and cloned genome components were infectious by biolistic inoculation to bean, completing koch's postulates for both. the b biotype of the whitefly bemisia tabaci efficiently transmitted both native virus and progeny virus derived from cloned dna inoculum. host ranges of native virus and of progeny virus der ...199918944770
tracing the geminivirus-whitefly transmission pathway by polymerase chain reaction in whitefly extracts, saliva, hemolymph, and honeydew.abstract a membrane feeding system and polymerase chain reaction (pcr) were used to track squash leaf curl virus (slcv) dna in whole whitefly body extracts and in saliva, honeydew, and hemolymph of its whitefly vector, bemisia tabaci, and a whitefly nonvector, trialeurodes vaporariorum. slcv ingestion was monitored by pcr in whiteflies that were given acquisition access periods (aaps) ranging from 0.5 to 96 h on virus-infected plants. slcv detection by pcr in whole body extracts was considered r ...199918944765
molecular characterization of the cucurbit yellow stunting disorder virus coat protein gene.abstract cucurbit yellow stunting disorder virus (cysdv) is a partially characterized bipartite closterovirus transmitted by the tobacco whitefly (bemisia tabaci). cysdv has emerged as a serious pathogen in southeastern spain and the mediterranean region, causing yellowing disease of cucumber and melon crops. using a modified reverse-transcription polymerase chain reaction protocol with gel-extracted dsrna templates, fragments of cysdv rna2 were amplified and cloned. sequence analysis of the clo ...199918944661
displacement of tomato yellow leaf curl virus (tylcv)-sr by tylcv-is in tomato epidemics in spain.abstract a progressive displacement of tomato yellow leaf curl virus (tylcv)-sr by tylcv-is was observed in tomato epidemics in southern spain based on incidence data of both virus species obtained during surveys conducted between 1996 and 1998. ecological factors that might be involved in such a displacement, such as competition of tylcv-sr and tylcv-is in tomato, transmission by local biotypes (b and q) of bemisia tabaci, and presence in weeds and alternate crops, have been analyzed. no select ...199918944659
watermelon chlorotic stunt virus from the sudan and iran: sequence comparisons and identification of a whitefly-transmission determinant.abstract the genomes of two watermelon chlorotic stunt virus (wmcsv) isolates, one from the sudan and one from iran, were cloned and sequenced. sequence relationship with other geminiviruses characterizes wmcsv as a typical eastern hemisphere geminivirus with a bipartite genome. the two geographically distant wmcsv isolates from africa and the middle east share a very high overall sequence similarity: 98% between their dna-a and 96% between their dna-b components, and their respective capsid pro ...200018944543
biological and molecular properties of a begomovirus from dicliptera sexangularis.abstract sixangle foldwing, dicliptera sexangularis (acanthaceae), showing severe yellow mottle and leaf distortion symptoms was collected from the shoreline of calusa island (lee county, fl). the putative virus was transmitted from infected d. sexangularis to healthy seedlings by mechanical, whitefly (bemisia tabaci biotype b), and graft-inoculations. different forms of geminivirus-like dnas were detected in total dna extracted from infected plants by southern blot hybridization analyses using ...200018944491
rate of tomato yellow leaf curl virus translocation in the circulative transmission pathway of its vector, the whitefly bemisia tabaci.abstract whiteflies (bemisia tabaci, biotype b) were able to transmit tomato yellow leaf curl virus (tylcv) 8 h after they were caged with infected tomato plants. the spread of tylcv during this latent period was followed in organs thought to be involved in the translocation of the virus in b. tabaci. after increasing acquisition access periods (aaps) on infected tomato plants, the stylets, the head, the midgut, a hemolymph sample, and the salivary glands dissected from individual insects were s ...200118944393
identification and characterization of a novel whitefly-transmitted member of the family potyviridae isolated from cucurbits in florida.abstract a novel whitefly-transmitted member of the family potyviridae was isolated from a squash plant (cucurbita pepo) with vein yellowing symptoms in florida. the virus, for which the name squash vein yellowing virus (sqvyv) is proposed, has flexuous rod-shaped particles of approximately 840 nm in length. the experimental host range was limited to species in the family cucurbitaceae, with the most dramatic symptoms observed in squash and watermelon, but excluded all tested species in the fami ...200718944369
tomato leaf curl karnataka virus from bangalore, india, appears to be a recombinant begomovirus.abstract the genome of tomato leaf curl virus (tolcv) from bangalore, india, a whitefly-transmitted geminivirus, was cloned (pind9) and sequenced. the circular dna of 2,759 nucleotides (u38239) is organized similarly to that of other begomoviruses with monopartite genomes. comparison of the nucleotide sequence of pind9 with other tomato-associated begomoviruses from india (tomato leaf curl bangalore virus [tolcbv, z48182]) and tomato leaf curl new delhi virus-severe (tolcndv-svr, u15015) showed ...200218944261
genetic structure of field populations of begomoviruses and of their vector bemisia tabaci in pakistan.abstract the genetic structure of field populations of begomoviruses and their whitefly vector bemisia tabaci in pakistan was analyzed. begomoviruses and b. tabaci populations were sampled from different crops and weeds in different locations in punjab and sindh provinces, in areas where cotton leaf curl disease (clcud) occurs or does not occur. phylogenetic analysis based on nucleotide sequences of the intergenic region in the viral dna-a provided evidence of two clusters of isolates: viruses i ...200318944071
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