Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| trypanosoma cruzi seroprevalence in pregnant women and screening by pcr and microhaematocrit in newborns from guanajuato, mexico. | chagas disease is caused by an infection with the protozoan hemoflagellate trypanosoma cruzi, and it is a major endemic health problem in latin america. the congenital route is one of the main non-vectorial pathways of transmission, which can arise either in the chronic or acute phase of maternal infection. serological screening of t. cruzi infection was performed in 520 pregnant women and newborns at the hospital general regional de león, guanajuato, mexico, between 2014 and 2015. anti-t. cruzi ... | 2016 | 27596439 |
| [clinical competence measurement in mexican resident physicians for diagnosis and treatment of chagas disease]. | evaluate clinical competence of a mexican resident physicians sample for diagnosis and treatment of chagas disease. | 2017 | 27595256 |
| effects of linalool and eugenol on the survival of leishmania (l.) infantum chagasi within macrophages. | the most commonly used drugs against visceral leishmaniasis are based on pentavalent antimonial compounds, which have played a fundamental role in therapy for over 70 years. however, the treatment is painful and has severe toxic side effects that can be fatal. antimonial resistance is spreading and reaching alarming proportions. linalool and eugenol have been shown to kill leishmania (l.) amazonensis and trypanosoma cruzi at low doses. in the present study, we demonstrate the effects of linalool ... | 2016 | 27591136 |
| mif-driven activation of macrophages induces killing of intracellular trypanosoma cruzi dependent on endogenous production of tumor necrosis factor, nitric oxide and reactive oxygen species. | the proinflammatory cytokine macrophage migration inhibitory factor (mif) is a key player in innate immunity. mif has been considered critical for controlling acute infection by the protozoan trypanosoma cruzi, but the underlying mechanisms are poorly understood. our study aimed to analyze whether mif could favor microbicidal activity of the macrophage, a site where t. cruzi grows and the initial effector cell against this parasite. using murine macrophages infected in vitro, we examined the eff ... | 2017 | 27591076 |
| effect of physical exercise training in patients with chagas heart disease: study protocol for a randomized controlled trial (peach study). | the effects of exercise training on chagas heart disease are still unclear. this study aimed to evaluate the effect of exercise training over functional capacity, cardiac function, quality of life, and biomarkers in chagas heart disease. | 2016 | 27590681 |
| analogues of marine guanidine alkaloids are in vitro effective against trypanosoma cruzi and selectively eliminate leishmania (l.) infantum intracellular amastigotes. | synthetic analogues of marine sponge guanidine alkaloids showed in vitro antiparasitic activity against leishmania (l.) infantum and trypanosoma cruzi. guanidines 10 and 11 presented the highest selectivity index when tested against leishmania. the antiparasitic activity of 10 and 11 was investigated in host cells and in parasites. both compounds induced depolarization of mitochondrial membrane potential, upregulation of reactive oxygen species levels, and increased plasma membrane permeability ... | 2016 | 27586460 |
| ecological scenario and trypanosoma cruzi dtu characterization of a fatal acute chagas disease case transmitted orally (espírito santo state, brazil). | trypanosoma cruzi infection via oral route results in outbreaks or cases of acute chagas disease (acd) in different brazilian regions and poses a novel epidemiological scenario. in the espírito santo state (southeastern brazil), a fatal case of a patient with acd led us to investigate the enzootic scenario to avoid the development of new cases. at the studied locality, triatoma vitticeps exhibited high t. cruzi infection rates and frequently invaded residences. | 2016 | 27580853 |
| first report of sapajus cay naturally infected by trypanosoma cruzi in san pedro department, paraguay. | to verify the occurrence of natural trypanosoma cruzi infection in non-human primates from a rural endemic area of the east region of paraguay, xenodiagnosis was performed in 35 animals belonging to two species. for genotyping and t. cruzi discrete typing unit (dtu) assignment, a combination of four markers was used, including amplification products of the small (18s) and large (24sα) subunits of ribosomal ribonucleic acid gene, the intergenic region of mini-exon gene and the heat shock protein ... | 2016 | 27579529 |
| glycogen synthase kinase-3 is involved in glycogen metabolism control and embryogenesis of rhodnius prolixus. | rhodnius prolixus is a blood-feeding insect that transmits trypanosoma cruzi and trypanosoma rangeli to vertebrate hosts. rhodnius prolixus is also a classical model in insect physiology, and the recent availability of r. prolixus genome has opened new avenues on triatomine research. glycogen synthase kinase 3 (gsk-3) is classically described as a key enzyme involved in glycogen metabolism, also acting as a downstream component of the wnt pathway during embryogenesis. gsk-3 has been shown to be ... | 2016 | 27574112 |
| translational activities to enable ntd vaccines. | there is an urgent need to develop new vaccines for tuberculosis, hiv/aids, and malaria, as well as for chronic and debilitating infections known as neglected tropical diseases (ntds). the term "ntd" emerged at the beginning of the new millennium to describe a set of diseases that are characterized as (1) poverty related, (2) endemic to the tropics and subtropics, (3) lacking public health attention and inadequate industrial investment, (4) having poor research funding and a weak research and de ... | 2016 | 27571699 |
| over six thousand trypanosoma cruzi strains classified into discrete typing units (dtus): attempt at an inventory. | trypanosoma cruzi, the causative agent of chagas disease, presents wide genetic diversity. currently, six discrete typing units (dtus), named tci to tcvi, and a seventh one called tcbat are used for strain typing. beyond the debate concerning this classification, this systematic review has attempted to provide an inventory by compiling the results of 137 articles that have used it. a total of 6,343 dtu identifications were analyzed according to the geographical and host origins. ninety-one perce ... | 2016 | 27571035 |
| unlocking the in vitro anti-trypanosoma cruzi activity of halophyte plants from the southern portugal. | to evaluate the in vitro anti-trypanosoma cruzi (t. cruzi) activity of organic extracts prepared from halophyte species collected in the southern coast of portugal (algarve), and chemically characterize the most active samples. | 2016 | 27569881 |
| antiprotozoal activity of extracts and isolated triterpenoids of 'carnauba' (copernicia prunifera) wax from brazil. | 'carnauba' wax is a natural product obtained from the processing of the powder exuded from copernicia prunifera (miller) h. e. moore (arecaceae). this material is widely used in the brazilian folk medicine, including the treatment of rheumatism and syphilis. | 2016 | 27569846 |
| [how much do medicine students know about chagas' disease in spain?] | 2017 | 27568170 | |
| molecular characterization of lipoamide dehydrogenase gene in trypanosoma cruzi populations susceptible and resistant to benznidazole. | lipoamide dehydrogenase (lipdh) is a flavin-containing disulfide oxidoreductase from the same group of thioredoxin reductase, glutathione reductase and trypanothione reductase. this enzyme is found in the mitochondria of all aerobic organisms where it takes part in at least three important multienzyme complexes from the citric acid cycle. in this study, we performed a phylogenetic analysis comparing the amino acid sequence of the lipdh from trypanosoma cruzi (tclipdh) with the lipdh from other o ... | 2016 | 27567984 |
| abo, secretor and lewis histo-blood group systems influence the digestive form of chagas disease. | chagas disease, caused by trypanosoma cruzi, can affect the heart, esophagus and colon. the reasons that some patients develop different clinical forms or remain asymptomatic are unclear. it is believed that tissue immunogenetic markers influence the tropism of t. cruzi for different organs. abo, secretor and lewis histo-blood group systems express a variety of tissue carbohydrate antigens that influence the susceptibility or resistance to diseases. this study aimed to examine the association of ... | 2016 | 27566333 |
| predominance of th1 response, increase of megakaryocytes and kupffer cells are related to survival in trypanosoma cruzi infected mice treated with lycopodium clavatum. | we investigated the number of megakaryocytes, kupffer cells and ratios of th1/th2 and th1/th17 cytokines in survival of mice infected with y strain of trypanosoma cruzi and treated with lycopodium clavatum. in a blind, randomized and controlled assay, swiss male mice, 8weeks-old, infected with 1400 trypomastigotes (y strain) were divided into groups and treated with: gly - lycopodium clavatum dynamization13c and gci - alcohol solution 7° gl (vehicle medicine). the treatment was offered two days ... | 2016 | 27565835 |
| scrutinizing the biomarkers for the neglected chagas disease: how remarkable! | biomarkers or biosignature profiles have become accessible over time in population-based studies for chagas disease. thus, the identification of consistent and reliable indicators of the diagnosis and prognosis of patients with heart failure might facilitate the prioritization of therapeutic management to those with the highest chance of contracting this disease. the purpose of this paper is to review the recent state and the upcoming trends in biomarkers for human chagas disease. as an emerging ... | 2016 | 27563302 |
| how do biting disease vectors behaviourally respond to host availability? | ecological theory predicts a diverse range of functional responses of species to resource availability; but in the context of human blood consumption by disease vectors, a simplistic, linear response is ubiquitously assumed. a simple and flexible model formulation is presented that extends the holling's types to account for a wider range of qualitatively distinct behaviours, and used to examine the impact of different vector responses to the relative availability of multiple blood-host species. | 2016 | 27562086 |
| morphological and phylogenetic description of trypanosoma noyesi sp. nov.: an australian wildlife trypanosome within the t. cruzi clade. | a number of trypanosome isolates from australian marsupials are within the clade containing the human pathogen trypanosoma cruzi. trypanosomes within this clade are thought to have diverged from a common ancestral bat trypanosome. here, we characterise trypanosoma noyesi sp. nov. isolated from the critically endangered woylie (bettongia pencillata) using phylogenetic inferences from three gene regions (18s rdna, ggapdh, and cytb) coupled with morphological and behavioural observations in vitro. ... | 2016 | 27561173 |
| taxonomic and predicted metabolic profiles of the human gut microbiome in pre-columbian mummies. | characterization of naturally mummified human gut remains could potentially provide insights into the preservation and evolution of commensal and pathogenic microorganisms, and metabolic profiles. we characterized the gut microbiome of two pre-columbian andean mummies dating to the 10-15th centuries using 16s rrna gene high-throughput sequencing and metagenomics, and compared them to a previously characterized gut microbiome of an 11th century ad pre-columbian andean mummy. our previous study sh ... | 2016 | 27559027 |
| evaluation of the novel antichagasic activity of [1,2,3]triazolo[1,5-a]pyridine derivatives. | trypanosoma cruzi is the causative agent of chagas disease. this parasite is vulnerable to the effects of ros as its main defense mechanism against exogenous agents trypanothione is also another weakness of the parasite that investigated related to the inhibition of enzymes belonging p450 system, mainly cyp51. in our group we have synthesized a series of triazoles known as [1,2,3]triazolo[1,5-a]pyridyl ketones, and pyridyl ketones. these families have shown interesting structural features due to ... | 2017 | 27558681 |
| flavonoids and chagas' disease: the story so far! | chagas disease is one of the major health problems in central and south america, which is caused by the parasitic protozoa, trypanosoma cruzi. it is commonly transmitted by members of blood-sucking subfamily triatominae. chagas disease is associated with cardiac and gastrointestinal manifestations. up to now, there are no effective vaccines for treatment of chagas disease and benznidazole and nifurtimox are the only effective anti-chagas drugs that cause different adverse and side effects. there ... | 2017 | 27558676 |
| [josé lima pedreira de freitas and the redefinition and control of chagas disease]. | a brief overview of the evolution of knowledge about chagas disease since its discovery by carlos chagas in 1909 until the mid-1940s is presented. the trajectory of physician pedreira de freitas and his growing involvement in research in the area led to his contributions to laboratory diagnosis - which lent consistency and security to epidemiological surveys of chagas disease - and the redefinition of the scale of the disease in brazil and the americas with its terrible social and economic impac ... | 2016 | 27557035 |
| expanding the knowledge of the chemical structure of glycoconjugates from trypanosoma cruzi tci genotype. contribution to taxonomic studies. | one of the main obstacles to the treatment of chagas disease is the genetic and phenotypical variance displayed by t. cruzi strains, resulting in differences in morphology, virulence, pathogenicity and drug susceptibility. to better understand the role of glycoconjungates in chagas disease, we performed the molecular characterization of the o-linked chains from mucins and glycoinositolphospholipids (gipls) of the silvio x10 clone 1 strain. we demonstrated the presence of a β-galactofuranose (β-g ... | 2016 | 27556227 |
| a local innate immune response against trypanosoma cruzi in the human placenta: the epithelial turnover of the trophoblast. | congenital chagas disease, caused by trypanosoma cruzi, is partially responsible for the progressive globalization of chagas disease despite of its low transmission rate. the probability of congenital transmission depends on complex interactions between the parasite, the maternal and fetus/newborn immune responses and placental factors, being the latter the least studied one. during transplacental transmission, the parasite must cross the placental barrier where the trophoblast, a continuous ren ... | 2016 | 27554274 |
| burden of heart failure in latin america: a systematic review and meta-analysis. | heart failure is a major public health concern. the aim of this review was to estimate the burden of heart failure in latin america. | 2016 | 27553287 |
| decreased cruzipain and gp85/trans-sialidase family protein expression contributes to loss of trypanosoma cruzi trypomastigote virulence. | two cell lines derived from a single trypanosoma cruzi clone by long-term passaging generated a highly virulent (c8c3hvir) and a low virulent (c8c3lvir) cell line. the c8c3hvir cell line was highly infective and lethal to balb/c mice, and the c8c3lvir cell line was three- to five-fold less infective to mouse cardiomyocytes than c8c3hvir. the highly virulent t. cruzi cell line abundantly expressed the major cysteine proteinase cruzipain (czp), complement regulatory protein (crp) and trans-sialida ... | 2017 | 27553285 |
| differential gel electrophoresis (dige) evaluation of naphthoimidazoles mode of action: a study in trypanosoma cruzi bloodstream trypomastigotes. | the obligate intracellular protozoan trypanosoma cruzi is the causative agent of chagas disease, a neglected illness affecting millions of people in latin america that recently entered non-endemic countries through immigration, as a consequence of globalization. the chemotherapy for this disease is based mainly on benznidazole and nifurtimox, which are very efficient nitroderivatives against the acute stage but present limited efficacy during the chronic phase. our group has been studying the tr ... | 2016 | 27551855 |
| systematic review and meta-analysis of the pharmacokinetics of benznidazole in the treatment of chagas disease. | chagas disease is a neglected parasitic illness affecting approximately 8 million people, predominantly in latin america. benznidazole is the drug of choice for treatment, although its availability has been limited. a paucity of knowledge of the pharmacokinetic properties of this drug has contributed to its limited availability in several jurisdictions. the objective of this study was to conduct a systematic literature review and a bayesian meta-analysis of pharmacokinetic studies to improve est ... | 2016 | 27550362 |
| hit-to-lead optimization of a novel class of potent, broad-spectrum trypanosomacides. | the parasitic trypanosomes trypanosoma brucei and t. cruzi are responsible for significant human suffering in the form of human african trypanosomiasis (hat) and chagas disease. drugs currently available to treat these neglected diseases leave much to be desired. herein we report optimization of a novel class of n-(2-(2-phenylthiazol-4-yl)ethyl)amides, carbamates, and ureas, which rapidly, selectively, and potently kill both species of trypanosome. the mode of action of these compounds is unknow ... | 2016 | 27548560 |
| evaluation of galectin-3 as a novel biomarker for chagas cardiomyopathy. | chagas cardiomyopathy has worse long-term outcomes than other cardiomyopathies. a biomarker strategy to refer subjects for noninvasive cardiac imaging may help in the early identification of cardiac damage in subjects with chagas disease. galectin-3 (gal-3) is a mediator of cardiac fibrosis shown to be upregulated in animal models of decompensated heart failure. here we assessed the correlation of gal-3 with myocardial fibrosis in patients with chagas disease. | 2017 | 27548475 |
| in vitro activities of pfaffia glomerata root extract, its hydrolyzed fractions and pfaffic acid against trypanosoma cruzi trypomastigotes. | this article reports on the in vitro activity of the hydroalcoholic extract of pfaffia glomerata roots, its hydrolyzed fractions, and pfaffic acid against trypanosoma cruzi. the hydroalcoholic extract obtained from dried, milled p. glomerata roots was submitted to acid hydrolysis followed by partition with chcl3 . the concentrated chcl3 fraction was suspended in meoh/h2 o and partitioned with hexane (f1), chcl3 (f2), and acoet (f3), in this sequence. the trypanocidal activity of the hydrolyzed e ... | 2017 | 27546206 |
| the role of primary infection of schwann cells in the aetiology of infective inflammatory neuropathies. | numerous different pathogens are responsible for infective peripheral neuropathies and this is generally the result of the indirect effects of pathogen infection, namely anti pathogen antibodies cross reacting with epitopes on peripheral nerve, auto reactive t cells attacking myelin, circulating immune complexes and complement fixation. primary infection of schwann cells (sc) associated with peripheral nerve inflammation is rare requiring pathogens to cross the blood peripheral nerve barrier (bp ... | 2016 | 27546064 |
| nitrotriazole-based acetamides and propanamides with broad spectrum antitrypanosomal activity. | 3-nitro-1h-1,2,4-triazole-based acetamides bearing a biphenyl- or a phenoxyphenyl moiety have shown remarkable antichagasic activity both in vitro and in an acute murine model, as well as substantial in vitro antileishmanial activity but lacked activity against human african trypanosomiasis. we have shown now that by inserting a methylene group in the linkage to obtain the corresponding propanamides, both antichagasic and in particular anti-human african trypanosomiasis potency was increased. th ... | 2016 | 27543881 |
| the role of ccr5 in chagas disease - a systematic review. | chagas disease is an infection caused by the protozoan trypanosoma cruzi. the clinical manifestations result from the chronic forms of the disease: indeterminate, cardiac, digestive or mixed. the pathogenesis of this disease is related to the genetic variability of both the parasite and the host with polymorphisms of genes involved in immune response possibly being involved in the variable clinical course. cytokines play a key role in regulating immune response, in particular chemokines exert a ... | 2016 | 27539514 |
| oesophageal motility disorders in infected immigrants with chagas disease in a non-endemic european area. | immigration-related new diseases pose a growing challenge for healthcare services in receptor countries. following latin american migration, chagas disease has inevitably appeared in europe. | 2016 | 27536373 |
| new bioactive semisynthetic derivatives of 31-norlanostenol and obtusifoliol from euphorbia officinarum. | fifteen semisynthetic terpenoid derivatives from the major latex components of euphorbia officinarum have been evaluated against the insect pest spodoptera littoralis, two species of protozoan parasites, trypanosoma cruzi and leishmania infantum, and also against insect sf9 and mammalian cho cells to test their selective cytotoxicity. our results showed that 40% of the test substances were postingestive toxicants to s. littoralis. all the tested derivatives had cytotoxic effects on insect-derive ... | 2016 | 27534104 |
| antiparasitic effect of dinoponera quadriceps giant ant venom. | neglected tropical diseases (ntd) are treated with toxic therapy of limited efficacy. previously, we studied the antimicrobial effect of dinoponera quadriceps venom (dqv) against bacteria. to continue the study, we report in this short communication the antimicrobial effect of dqv against leishmania amazonensis and trypanosoma cruzi. dqv inhibits the promastigote forms of l. amazonensis and all t. cruzi developmental forms, with low toxicity in host cells. dqv causes cell death in t. cruzi throu ... | 2016 | 27530664 |
| the new incurable wound. | 2016 | 27529365 | |
| alkaline phosphatase activity in salivary gland cells of rhodnius neglectus and r. prolixus (hemiptera, triatominae). | alkaline phosphatase activity was detected in salivary gland cells of the rhodnius neglectus lent, 1954, and r. prolixus stal, 1859, vectors of trypanosoma cruzi chagas, 1909 (etiological agent of chagas disease) and t. rangeli tejera, 1920 (pathogenic to insect). the gomori technique was used to demonstrate alkaline phosphatase activity. alkaline phosphatase activity was observed throughout the entire gland, with an increased activity in the posterior region of the principal gland. in particula ... | 2016 | 27525888 |
| chagas disease: a solvable problem, ignored. | chagas disease is the highest impact parasitic disease in the americas, yet remains virtually unknown and untreated, despite the fact that the infection is curable and the global problem of chagas disease is manageable. the causes of this situation and how it can be changed are the focus of this communication. | 2016 | 27523778 |
| distribution of serological screening markers at a large hematology and hemotherapy center in minas gerais, southeastern brazil. | to assess the distribution of serological markers in blood donors at the blood banks of the fundação centro de hematologia e hemoterapia de minas gerais (hemominas), brazil, between january 2006 and december 2012. | 2016 | 27521858 |
| performance assessment of four chimeric trypanosoma cruzi antigens based on antigen-antibody detection for diagnosis of chronic chagas disease. | the performance of serologic tests in chronic chagas disease diagnosis largely depends on the type and quality of the antigen preparations that are used for detection of anti-trypanosoma cruzi antibodies. whole-cell t. cruzi extracts or recombinant proteins have shown variation in the performance and cross-reactivity. synthetic chimeric proteins comprising fragments of repetitive amino acids of several different proteins have been shown to improve assay performances to detect chagasic infections ... | 2016 | 27517281 |
| congenital chagas' disease transmission in the united states: diagnosis in adulthood. | two brothers with congenitally-acquired chagas' disease (cd) diagnosed during adulthood are reported. the patients were born in the usa to a mother from bolivia who on subsequent assessment was found to be serologically positive for trypanosoma cruzi. serologic screening of all pregnant women who migrated from countries with endemic cd is strongly recommended. | 2016 | 27516969 |
| habitat management to reduce human exposure to trypanosoma cruzi and western conenose bugs (triatoma protracta). | chagas disease, which manifests as cardiomyopathy and severe gastrointestinal dysfunction, is caused by trypanosoma cruzi, a vector-borne parasite. in california, the vector triatoma protracta frequently colonizes woodrat (neotoma spp.) lodges, but may also invade nearby residences, feeding upon humans and creating the dual risk of bite-induced anaphylaxis and t. cruzi transmission. our research aimed to assess t. cruzi presence in woodrats in a previously unstudied northern california area, sta ... | 2016 | 27515118 |
| detection and quantification of leishmania infantum in naturally and experimentally infected animal samples. | leishmania infantum is one of the causative agents of visceral leishmaniasis (vl). vl is the most severe form of leishmaniasis and can be fatal if it is not properly treated. although several pcr works are intended to detect l. infantum, in silico analysis of available primers and/or primer-probes reveals potential cross species amplification. here, a taqman-based quantitative real time pcr (qpcr) assay was developed for specific detection and quantitation of l. infantum in tissue samples from e ... | 2016 | 27514885 |
| high-resolution molecular typing of trypanosoma cruzi in 2 large outbreaks of acute chagas disease in colombia. | oral transmission of trypanosoma cruzi has gained relevance because of its association with high morbidity and lethality rates. this transmission route is responsible for maintaining the infection of the parasite in sylvatic cycles, and human cases have been associated mainly with the consumption of food contaminated with triatomine feces or didelphid secretions. several ecological changes allow the intrusion of sylvatic reservoirs and triatomines to the domestic environments with subsequent foo ... | 2016 | 27511897 |
| community resilience and chagas disease in a rural region of mexico. | to explore the pillars of community resilience in a region where chagas disease is endemic, with the aim of promoting participatory processes to deal with this condition from the resilience of the population. | 2016 | 27509012 |
| comparative transcriptome profiling of human foreskin fibroblasts infected with the sylvio and y strains of trypanosoma cruzi. | trypanosoma cruzi, the causative agent of chagas disease, is phylogeneticaly distributed into nearly identical genetic strains which show divergent clinical presentations including differences in rates of cardiomyopathy in humans, different vector species and transmission cycles, differential congenital transmission in a mouse model, and differing immune and heart inflammation response in dogs. the population structure of these strains divides into two groups, which are geographically and clinic ... | 2016 | 27505626 |
| cutaneous presentation of chagas' disease reactivation in a heart-transplant patient. | 2017 | 27504862 | |
| synthesis and trypanocidal activity of novel benzimidazole derivatives. | the present work reports the synthesis and biological activity of a series of 14 benzimidazole derivatives designed to act on the enzyme triosephosphate isomerase of trypanosoma cruzi (tctim). this enzyme is involved in the metabolism of glucose, the only source of energy for the parasite. in this study, we found four compounds that inhibit tctim moderately and lack inhibitory activity against human tim (hstim). in vitro studies against t. cruzi epimastigotes showed two compounds that were more ... | 2016 | 27503677 |
| conservation and divergence within the clathrin interactome of trypanosoma cruzi. | trypanosomatids are parasitic protozoa with a significant burden on human health. african and american trypanosomes are causative agents of nagana and chagas disease respectively, and speciated about 300 million years ago. these parasites have highly distinct life cycles, pathologies, transmission strategies and surface proteomes, being dominated by the variant surface glycoprotein (african) or mucins (american) respectively. in african trypanosomes clathrin-mediated trafficking is responsible f ... | 2016 | 27502971 |
| evaluation of vdr gene polymorphisms in trypanosoma cruzi infection and chronic chagasic cardiomyopathy. | vitamin d is an important modulator of the immune response. it acts over several immune cell types where the vitamin d receptor (vdr) is expressed. due to the high relevance of this signaling pathway, several studies have investigated the possible influence of genes involved in the metabolism of vitamin d and its receptor in different human diseases. here, we analyzed whether four single-nucleotide polymorphisms of the vdr gene (rs731236, rs7975232, rs1544410 and rs2228570) are involved in the s ... | 2016 | 27502545 |
| proteasome inhibition for treatment of leishmaniasis, chagas disease and sleeping sickness. | chagas disease, leishmaniasis and sleeping sickness affect 20 million people worldwide and lead to more than 50,000 deaths annually. the diseases are caused by infection with the kinetoplastid parasites trypanosoma cruzi, leishmania spp. and trypanosoma brucei spp., respectively. these parasites have similar biology and genomic sequence, suggesting that all three diseases could be cured with drugs that modulate the activity of a conserved parasite target. however, no such molecular targets or br ... | 2016 | 27501246 |
| hiv aspartic peptidase inhibitors are effective drugs against the trypomastigote form of the human pathogen trypanosoma cruzi. | there is a general lack of effective and non-toxic chemotherapeutic agents against chagas' disease despite more than a century of research. in this regard, we have verified the impact of human immunodeficiency virus aspartic peptidase inhibitors (hiv-pis) on the viability and morphology of infective trypomastigote forms of trypanosoma cruzi as well as on the aspartic peptidase and proteasome activities produced by this parasite. the effects of hiv-pis on viability were assessed by counting motil ... | 2016 | 27499433 |
| hypothesis testing clarifies the systematics of the main central american chagas disease vector, triatoma dimidiata (latreille, 1811), across its geographic range. | the widespread and diverse triatoma dimidiata is the kissing bug species most important for chagas disease transmission in central america and a secondary vector in mexico and northern south america. its diversity may contribute to different chagas disease prevalence in different localities and has led to conflicting systematic hypotheses describing various populations as subspecies or cryptic species. to resolve these conflicting hypotheses, we sequenced a nuclear (internal transcribed spacer 2 ... | 2016 | 27496718 |
| the n-myristoylome of trypanosoma cruzi. | protein n-myristoylation is catalysed by n-myristoyltransferase (nmt), an essential and druggable target in trypanosoma cruzi, the causative agent of chagas' disease. here we have employed whole cell labelling with azidomyristic acid and click chemistry to identify n-myristoylated proteins in different life cycle stages of the parasite. only minor differences in fluorescent-labelling were observed between the dividing forms (the insect epimastigote and mammalian amastigote stages) and the non-di ... | 2016 | 27492267 |
| zoonotic trypanosomes in south east asia: attempts to control trypanosoma lewisi using human and animal trypanocidal drugs. | beside typical human trypanosomes responsible of sleeping sickness in africa and chagas disease in latin america, there is a growing number of reported atypical human infections due to trypanosoma evansi, a livestock parasite, or trypanosoma lewisi, a rat parasite, especially in asia. drugs available for the treatment of t. brucei ssp. in humans are obviously of choice for the control of t. evansi because it is derived from t. brucei. however, concerning t. lewisi, there is an urgent need to det ... | 2016 | 27491458 |
| functional evaluation of heat shock proteins 70 (hsp70/hsc70) on rhodnius prolixus (hemiptera, reduviidae) physiological responses associated with feeding and starvation. | blood-sucking vectors must overcome thermal stress caused by intake of proportionally large amounts of warm blood from their hosts. in response to this, heat shock proteins (hsps) such as the widely studied hsp70 family (the inducible hsp70 and the cognate form hsc70, known for their role in preserving essential cellular functions) are rapidly up-regulated in their tissues. the triatomine rhodnius prolixus is an important vector of trypanosoma cruzi, the causative pathogen of chagas' disease, an ... | 2016 | 27491440 |
| lipid body organelles within the parasite trypanosoma cruzi: a role for intracellular arachidonic acid metabolism. | most eukaryotic cells contain varying amounts of cytosolic lipidic inclusions termed lipid bodies (lbs) or lipid droplets (lds). in mammalian cells, such as macrophages, these lipid-rich organelles are formed in response to host-pathogen interaction during infectious diseases and are sites for biosynthesis of arachidonic acid (aa)-derived inflammatory mediators (eicosanoids). less clear are the functions of lbs in pathogenic lower eukaryotes. in this study, we demonstrated that lbs, visualized b ... | 2016 | 27490663 |
| interactions between 4-aminoquinoline and heme: promising mechanism against trypanosoma cruzi. | chagas disease is a neglected tropical disease caused by the flagellated protozoan trypanosoma cruzi. the current drugs used to treat this disease have limited efficacy and produce severe side effects. quinolines, nitrogen heterocycle compounds that form complexes with heme, have a broad spectrum of antiprotozoal activity and are a promising class of new compounds for chagas disease chemotherapy. in this study, we evaluated the activity of a series of 4-arylaminoquinoline-3-carbonitrile derivati ... | 2016 | 27490082 |
| success of benznidazole chemotherapy in chronic trypanosoma cruzi-infected patients with a sustained negative pcr result. | cure assessment in chronic trypanosoma cruzi infection is controversial, mainly because of the lack of reliable tests to ensure parasite elimination. here, we assess the impact of benznidazole therapy on the conventional serology and parasitaemia in chronic chagas disease. a total of 455 patients with long-term trypanosoma cruzi infection underwent specific chemotherapy with benznidazole. their parasitological status was assessed by polymerase chain reaction (pcr) detection of t. cruzi dna. drop ... | 2016 | 27488437 |
| preparation of benznidazole pellets for immediate drug delivery using the extrusion spheronization technique. | recent advances in the treatment of chagas disease have followed combinations of drugs that act synergistically against infection, predominantly including benznidazole (bnz) and azoles derivatives. possible incompatibilities between these drugs, slow dissolution of bnz and dose adjustment difficulties are technological obstacles to the development of multidrug formulations. thus, in the present study, bnz pellets were developed using extrusion spheronization for immediate drug delivery. preformu ... | 2017 | 27487264 |
| the sympathetic nervous system affects the susceptibility and course of trypanosoma cruzi infection. | trypanosoma cruzi (t. cruzi) is an intracellular parasite that causes chagas' disease, a major health problem in latin america. using a murine model of infection with this parasite, we have previously shown that corticosterone blood levels are markedly elevated during the course of the disease in c57bl/6 male mice and that this increase is protective for the host by restricting the production of pro-inflammatory cytokines. since the hypothalamus-pituitary-adrenal (hpa) axis usually operates in a ... | 2016 | 27485039 |
| 1,3,4-thiadiazole derivatives of r-(+)-limonene benzaldehyde-thiosemicarbazones cause death in trypanosoma cruzi through oxidative stress. | this work evaluated the in vitro and in vivo activity of tdz 2 on trypanosoma cruzi amastigotes and determined the possible mechanism of action of this compound on t. cruzi death. tdz 2 inhibited t. cruzi proliferation in vitro and had low haemolytic potential. it also induced morphological and ultrastructural alterations. we observed a reduction of cell volume, the depolarization of the mitochondrial membrane, an increase in ros production, lipoperoxidation of the cell membrane, lipid bodies fo ... | 2016 | 27484335 |
| surface molecules released by trypanosoma cruzi metacyclic forms downregulate host cell invasion. | the question whether metacylic trypomastigote (mt) forms of different t. cruzi strains differentially release surface molecules, and how they affect host cell invasion, remains to be fully clarified. we addressed that question using t. cruzi strains that differ widely in the ability to invade cells. | 2016 | 27483135 |
| the aryl hydrocarbon receptor modulates production of cytokines and reactive oxygen species and development of myocarditis during trypanosoma cruzi infection. | the aryl hydrocarbon receptor (ahr) is a ligand-activated transcription factor involved in controlling several aspects of immune responses, including the activation and differentiation of specific t cell subsets and antigen-presenting cells, thought to be relevant in the context of experimental trypanosoma cruzi infection. the relevance of ahr for the outcome of t. cruzi infection is not known and was investigated here. we infected wild-type (wt) mice and ahr knockout (ahr ko) mice with t. cruzi ... | 2016 | 27481250 |
| loss of the cytostome-cytopharynx and endocytic ability are late events in trypanosoma cruzi metacyclogenesis. | trypanosoma cruzi epimastigotes uptake nutrients by endocytosis via the cytostome-cytopharynx complex - an anterior opening (cytostome) continuous with a funnel-shaped invagination (cytopharynx) that extends to the posterior of the cell, accompanied by microtubules. during metacyclogenesis - the transformation of epimastigotes into human-infective metacyclic trypomastigotes - the cytostome-cytopharynx complex disappears, as trypomastigotes lose endocytic ability. to date, no studies have examine ... | 2016 | 27480509 |
| tyrosine detoxification is an essential trait in the life history of blood-feeding arthropods. | blood-feeding arthropods are vectors of infectious diseases such as dengue, zika, chagas disease, and malaria [1], and vector control is essential to limiting disease spread. because these arthropods ingest very large amounts of blood, a protein-rich meal, huge amounts of amino acids are produced during digestion. previous work on rhodnius prolixus, a vector of chagas disease, showed that, among all amino acids, only tyrosine degradation enzymes were overexpressed in the midgut compared to other ... | 2016 | 27476595 |
| design, synthesis, molecular docking and biological evaluation of thiophen-2-iminothiazolidine derivatives for use against trypanosoma cruzi. | in this study, we designed and synthesized a series of thiophen-2-iminothiazolidine derivatives from thiophen-2-thioureic with good anti-trypanosoma cruzi activity. several of the final compounds displayed remarkable trypanocidal activity. the ability of the new compounds to inhibit the activity of the enzyme cruzain, the major cysteine protease of t. cruzi, was also explored. the compounds 3b, 4b, 8b and 8c were the most active derivatives against amastigote form, with significant ic50 values b ... | 2016 | 27475533 |
| [screening of parasitic diseases in the asymptomatic immigrant population]. | parasitic diseases suppose an important health problem in people from high endemic areas, so these must be discarded properly. usually, these infections develop asymptomatically but, in propitious situations, are likely to reactivate themselves and can cause clinical symptoms and/or complications in the receiving country. moreover, in some cases it is possible local transmission. early diagnosis of these parasitic diseases made by appropriate parasitological techniques and its specific treatment ... | 2016 | 27474244 |
| dendritic cells: a double-edged sword in immune responses during chagas disease. | dendritic cells (dcs) are the most important member of the antigen presenting cells group due to their ability to recognize antigen at the infection site and their high specialized antigen internalization capacity. these cells have central role in connecting the innate and adaptive immune responses against trypanosoma cruzi, the causative agent of chagas disease. these first line defense cells modulate host immune response depending on type, maturation level, cytokine milieu and dc receptor invo ... | 2016 | 27471496 |
| blind dockings of benzothiazoles to multiple receptor conformations of triosephosphate isomerase from trypanosoma cruzi and human. | we aim to uncover the binding modes of benzothiazoles, which have been reported as specific inhibitors of triosephosphate isomerase from the parasite trypanosoma cruzi (tctim), by performing blind dockings on both tctim and human tim (htim). detailed analysis of binding sites and specific interactions are carried out based on ensemble dockings to multiple receptor conformers obtained from molecular dynamics simulations. in tctim dimer dockings, the inhibitors preferentially bind to the tunnel-sh ... | 2011 | 27468153 |
| usefulness of microvolt t-wave alternans for predicting outcome in patients with chagas disease with implantable cardioverter defibrillators. | chagas disease (chd) may lead to life-threatening heart disease, including malignant ventricular arrhythmias. the use of implantable cardioverter defibrillators (icds) has become the main therapeutic strategy for secondary prevention of scd in chagas disease (chd). microvolt t-wave alternans (mtwa) is a direct measure of ventricular repolarization instability and has emerged as a potentially useful way of determining arrhythmia vulnerability. however, this methodology has not been evaluated in p ... | 2016 | 27467316 |
| integration of ligand- and target-based virtual screening for the discovery of cruzain inhibitors. | a myriad of methods are available for virtual screening of small organic compound databases. in this study we have successfully applied a quantitative model of consensus measurements, using a combination of 3d similarity searches (rocs and eon), hologram quantitative structure activity relationships (hqsar) and docking (fred, flexx, glide and autodock vina), to retrieve cruzain inhibitors from collected databases. all methods were assessed individually and then combined in a ligand-based virtual ... | 2011 | 27467157 |
| diagnosis and treatment of congenital chagas disease in a premature infant. | 2016 | 27466398 | |
| differentiation of trypanosoma cruzi i (tci) and t. cruzi ii (tcii) genotypes using genes encoding serine carboxypeptidases. | the parasite trypanosoma cruzi (kinetoplastida, trypanosomatidae) can be classified based on biochemical and molecular markers, into six lineages or discrete typing units (dtus), t. cruzi i-vi (tci-vi), from which tci and tcii are the parental genotypes. trying to understand the dispersion of the subpopulations of t. cruzi in nature and its complex transmission cycles, the serine carboxypeptidase genes of t. cruzi were used as a molecular marker in the present study. dtus of 25 t. cruzi isolates ... | 2016 | 27465563 |
| is the antitumor property of trypanosoma cruzi infection mediated by its calreticulin? | eight to 10 million people in 21 endemic countries are infected with trypanosoma cruzi. however, only 30% of those infected develop symptoms of chagas' disease, a chronic, neglected tropical disease worldwide. similar to other pathogens, t. cruzi has evolved to resist the host immune response. studies, performed 80 years ago in the soviet union, proposed that t. cruzi infects tumor cells with similar capacity to that displayed for target tissues such as cardiac, aortic, or digestive. an antagoni ... | 2016 | 27462315 |
| identification of three cytochrome p450 genes in the chagas' disease vector triatoma infestans: expression analysis in deltamethrin susceptible and resistant populations. | cytochrome p450 monooxygenases play a predominant role in the metabolism of insecticides. many insect p450 genes have frequently been associated with detoxification processes allowing the insect to become tolerant or resistant to insecticides. the increases of expression of p450 genes at transcriptional level are often consider responsible for increasing the metabolism of insecticides and seems to be a common phenomenon in the evolution of resistance development in insects. as pyrethroid resista ... | 2016 | 27461853 |
| the characterization of anti-t. cruzi activity relationships between ferrocenyl, cyrhetrenyl complexes and ros release. | trypanosoma cruzi (t. cruzi) is the parasite that causes chagas disease. nifurtimox is the most used drug against the t. cruzi, this drug increases intermediaries nitro group, being mainly responsible for the high toxicity component, for this reason it is important to study new organic compounds and thus improve therapeutic strategies against chagas disease. the electronic effects of ferrocenyl and cyrhetrenyl fragments were investigated by dft calculation. a close correlation was found between ... | 2016 | 27460450 |
| humoral and cellular immune responses induced by the urease-derived peptide jaburetox in the model organism rhodnius prolixus. | although the entomotoxicity of plant ureases has been reported almost 20 years ago, their insecticidal mechanism of action is still not well understood. jaburetox is a recombinant peptide derived from one of the isoforms of canavalia ensiformis (jack bean) urease that presents biotechnological interest since it is toxic to insects of different orders. previous studies of our group using the chagas disease vector and model insect rhodnius prolixus showed that the treatment with jack bean urease ( ... | 2016 | 27455853 |
| indeterminate form of chagas disease: is left ventricular torsional mechanics a clue to subclinical myocardial abnormalities? | chagas disease (cd) is highly endemic in latin america, and has been emerging in developed countries. early diagnosis of left ventricular (lv) systolic dysfunction, routinely done by echocardiography, is crucial for therapy. speckle tracking echocardiography allows determination of lv segmental rotations, twist/torsion, and this analysis may disclose an unapparent myocardial contractile deficit in initial stages of cd. therefore, this study aimed to highlight a possible unapparent myocardial con ... | 2017 | 27450558 |
| trypanocidal activity of flavokawin b, a component of polygonum ferrugineum wedd. | the trypanocidal potential of the natural chalcone flavokawin b, which was isolated from the hexanic extract of polygonum ferrugineum wedd., is reported here. although flavokawin b is widespread, this is the first report about its trypanocidal properties on both trypanosoma cruzi (ic50 = 9.5 µm, ic50 = 34.7 µm benznidazol, y strain) epimastigotes and trypanosoma brucei (ic50 = 4.8 µm, ic50 = 6.4 µm pentamidine, 29-13 strain) procyclic forms, which was also corroborated on t. brucei strain 427 (i ... | 2017 | 27442262 |
| importation of hybrid human-associated trypanosoma cruzi strains of southern south american origin, colombia. | we report the characterization of trypanosoma cruzi of southern south american origin among humans, domestic vectors, and peridomestic hosts in colombia using high-resolution nuclear and mitochondrial genotyping. expanding our understanding of the geographic range of lineage tcvi, which is associated with severe chagas disease, will help clarify risk of human infection for improved disease control. | 2016 | 27434772 |
| chagas disease screening in maternal donors of publicly banked umbilical cord blood, united states. | to assess patterns of chagas disease, we reviewed results of screening umbilical cord blood from a us public cord blood bank during 2007-2014. nineteen maternal donors tested positive for trypanosoma cruzi parasites (0.04%). because perinatal transmission of chagas disease is associated with substantial illness, targeted prenatal programs should screen for this disease. | 2016 | 27433974 |
| safety profile of nifurtimox for treatment of chagas disease in the united states. | nifurtimox is 1 of only 2 medications available for treating chagas disease (cd) and currently the only drug available in the united states, but its safety and tolerance have not been extensively studied. this is the first study to evaluate tolerance of nifurtimox in us patients with cd. | 2016 | 27432838 |
| an aromatic diamidine that targets kinetoplast dna, impairs the cell cycle in trypanosoma cruzi, and diminishes trypomastigote release from infected mammalian host cells. | trypanosoma cruzi is the etiological agent of chagas disease, affecting approximately 10 million people in the americas and with some 40 million people at risk. the objective of this study was to evaluate the anti-t. cruzi activity of three new diamidines that have a 3,4-ethylenedioxy extension of the thiophene core, designated mb17, mb19, and mb38. all three diamidines exhibited dose-dependent inhibition of epimastigote replication. the mechanisms of action of these diamidines were investigated ... | 2016 | 27431229 |
| pathology of chronic chagas cardiomyopathy in the united states: a detailed review of 13 cardiectomy cases. | the pathologic features of chronic chagas cardiomyopathy may not be widely appreciated in the united states. we sought to describe the gross, microscopic, immunohistochemical, and molecular pathology features useful to diagnose chronic chagas cardiomyopathy. | 2016 | 27425387 |
| new record and cytogenetic analysis of psammolestes tertius lent & jurberg, 1965 (hemiptera, reduviidae, triatominae) from bahia state, brazil. | this paper reports on the first occurrence of psammolestes tertius in the chapada diamantina region, located in the city of seabra, bahia state, in northeastern brazil. following an active search, 24 p. tertius specimens were collected from phacellodomus rufifrons (rufous-fronted thornbird) nests. the insects did not present any symptoms of infection by trypanosoma cruzi. p. tertius males were cytogenetically analyzed, and the results were compared with those of other specimens from the brazilia ... | 2016 | 27420943 |
| antagonistic effect of atorvastatin on high fat diet induced survival during acute chagas disease. | chagasic cardiomyopathy, which is seen in chagas disease, is the most severe and life-threatening manifestation of infection by the kinetoplastid trypanosoma cruzi. adipose tissue and diet play a major role in maintaining lipid homeostasis and regulating cardiac pathogenesis during the development of chagas cardiomyopathy. we have previously reported that t. cruzi has a high affinity for lipoproteins and that the invasion rate of this parasite increases in the presence of cholesterol, suggesting ... | 2016 | 27416748 |
| immunopathological features of canine myocarditis associated with leishmania infantum infection. | myocarditis associated with infectious diseases may occur in dogs, including those caused by the protozoa neospora caninum, trypanosoma cruzi, babesia canis, and hepatozoon canis. however, although cardiac disease due to leishmania infection has also been documented, the immunopathological features of myocarditis have not been reported so far. the aim of this study was to examine the types of cellular infiltrates and expression of mhc classes i and ii in myocardial samples obtained at necropsy f ... | 2016 | 27413751 |
| circulation of tc ia discrete type unit trypanosoma cruzi in yucatan mexico. | the etiologic agent trypanosoma cruzi (tc) has been grouped into six discrete type units (dtu i-vi); within dtu-i exists four subgroups defined ia-id. in colombia, the genotype ia is associated with human infection and domiciliated rhodnius vector. in the yucatan peninsula of mexico, the main vector involved in t. cruzi transmission is triatoma dimidiata predominantly via sylvatic and peridomiciliated cycles. in this study, multiple sequence analysis of mini-exon intergenic regions of t. cruzi i ... | 2016 | 27413339 |
| clinical course after cardioverter-defibrillator implantation: chagasic versus ischemic patients. | the outcome of chagas disease patients after receiving implantable cardioverter defibrillator (icd) is still controversial. | 2016 | 27411097 |
| correlation between infection rate of triatominies and chagas disease in southwest of bahia, brazil: a warning sign? | chagas disease, caused by the trypanosoma cruzi, has a wide distribution in south america, and its main method of control is the elimination of triatomines. it is presented here the geographic distribution and the rate of natural infection by t. cruzi of triatomines collected and evaluated from 2008 to 2013 in southwest of bahia. triatomines were captured in the intradomiciliary and peridomiciliary areas of five cities located in the southwest of bahia state, identified, and analyzed for the pre ... | 2016 | 27411071 |
| the role of gender in chagas disease prevention and control in honduras: an analysis of communication and collaboration networks. | in honduras, where chagas disease is a serious health and environmental concern, prevention measures face the challenge of achieving widespread and long-term sustainable adoption by communities. the article integrates social network analysis and a gender-sensitive approach to understand the role of men and women in the implementation of a community-level intervention, based on the adoption of housing improvements to reduce the presence of the insect vector. a total of 108 people in the community ... | 2016 | 27405994 |
| leishmanicidal activity of peroxynitrite. | nitric oxide reacts with superoxide to produce peroxynitrite which has been reported to be highly microbicidal to trypanosoma cruzi in phosphate buffer but ineffective against leishmania major in culture medium. this contradiction and the potential importance of peroxynitrite as a cytotoxic effector molecule of both macrophages and neutrophils led us to re-examine its leishmanicidal effects. our results demonstrate that peroxynitrite inhibits growth of leishmania amazonensis promastigotes in a c ... | 1995 | 27405713 |
| product release pathways in human and plasmodium falciparum phosphoribosyltransferase. | atomistic molecular dynamics (md) simulations coupled with the metadynamics technique were carried out to delineate the product (ppi.2mg and imp) release mechanisms from the active site of both human (hs) and plasmodium falciparum (pf) hypoxanthine-guanine-(xanthine) phosphoribosyltransferase (hg(x)prt). an early movement of ppi.2mg from its binding site has been observed. the swinging motion of the asp side chain (d134/d145) in the binding pocket facilitates the detachment of imp, which trigger ... | 2016 | 27404508 |
| selective testing of at-risk blood donors for trypanosoma cruzi and plasmodium spp. in switzerland. | population migrations and overseas recreational travel to regions at risk for tropical diseases are increasing. a major challenge in non-endemic countries is to decrease the number of blood donor deferrals due those tropical disease pathogens, without compromising the high level of blood safety. the protozoans trypanosoma cruzi and plasmodium spp., the causative organisms of chagas disease (cd) and malaria are becoming a major focus in the blood transfusion community. | 2016 | 27403088 |
| what do we know about chagas disease in the united states? | chagas disease, caused by the parasite trypanosoma cruzi, affects more than 5 million people worldwide leading to serious heart and gastrointestinal disease in a proportion of chronically infected patients. important modes of transmission include vector-borne, congenital, and via blood transfusion or organ transplant from an infected donor. vector-borne transmission of chagas disease occurs in the americas, including the southern half of north america, where the specific vector insects (triatomi ... | 2016 | 27402515 |