Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| chemical composition profiling and antifungal activity of the essential oil and plant extracts of mesembryanthemum edule (l.) bolus leaves. | essential oil from mesembryanthemum edule leaves have been used by the eastern cape traditional healers for the treatment of respiratory tract infections, tuberculosis, dysentery, diabetic mellitus, laryngitis and vaginal infections. the investigation of bioactive compounds in the essential oil of this plant could help to verify the efficacy of the plant in the management or treatment of these illnesses. | 2014 | 25392576 |
| weekly use of fluconazole as prophylaxis in haematological patients at risk for invasive candidiasis. | the goal was to determine whether one medical centres' unique antifungal prophylactic regimen for patients at high risk for invasive candidiasis because of their haematological malignancies, haematopoietic stem cell transplants, or high-dose chemotherapy might lead ultimately to a higher incidence of infection, to increasing fluconazole resistance, or to a shift in the predominant strain of candida in invasive fungal episodes. | 2014 | 25384689 |
| inhibitory activity in vitro of probiotic lactobacilli against oral candida under different fermentation conditions. | clinical studies have shown that probiotics positively affect oral health by decreasing gum bleeding and/or reducing salivary counts of certain oral pathogens. our aim was to investigate the inhibitory effect of six probiotic lactobacilli against opportunistic oral candida species. sugar utilisation by both lactobacilli and candida was also assessed. agar overlay assay was utilised to study growth inhibition of candida albicans, candida glabrata and candida krusei by lactobacillus rhamnosus gg, ... | 2015 | 25380800 |
| one small step for a yeast--microevolution within macrophages renders candida glabrata hypervirulent due to a single point mutation. | candida glabrata is one of the most common causes of candidemia, a life-threatening, systemic fungal infection, and is surpassed in frequency only by candida albicans. major factors contributing to the success of this opportunistic pathogen include its ability to readily acquire resistance to antifungals and to colonize and adapt to many different niches in the human body. here we addressed the flexibility and adaptability of c. glabrata during interaction with macrophages with a serial passage ... | 2014 | 25356907 |
| resistance reversal induced by a combination of fluconazole and tacrolimus (fk506) in candida glabrata. | there is an increasing concern about candida glabrata due to its high isolation frequency in candidiasis recently and notorious drug resistance to fluconazole. drug combination is one effective approach to counteract drug resistance. this study aimed to test whether a combination of fluconazole and tacrolimus (fk506) had a synergistic effect on c. glabrata, and to seek the potential mechanisms underlying the synergistic effects. in vitro effects of fluconazole and fk506 against c. glabrata with ... | 2015 | 25355935 |
| fluconazole resistance patterns in candida species that colonize women with hiv infection. | the women's interagency hiv study was established in 1993 to study the natural history of hiv disease among women in the united states. it currently has enrolled 2,895 women testing positive for hiv infection and 972 women without hiv infection recruited from 6 national metropolitan locations. the clinical database information collected for each hiv-positive individual included cd4 cell counts, viral load, and antiviral treatment to evaluate hiv prognosis and related conditions in women. | 2014 | 25352939 |
| possible mechanism of antifungal phenazine-1-carboxamide from pseudomonas sp. against dimorphic fungi benjaminiella poitrasii and human pathogen candida albicans. | investigation of antifungal mechanism of phenazine 1-carboxamide (pc) produced by a pseudomonas strain mcc2142. | 2015 | 25348290 |
| drug-resistant candida glabrata infection in cancer patients. | cancer patients are at risk for candidemia, and increasing candida spp. resistance poses an emerging threat. we determined rates of antifungal drug resistance, identified factors associated with resistance, and investigated the correlation between resistance and all-cause mortality rates among cancer patients with ≥1 c. glabrata-positive blood culture at md anderson cancer center, houston, texas, usa, during march 2005-september 2013. of 146 isolates, 30 (20.5%) were resistant to fluconazole, 15 ... | 2014 | 25340258 |
| serum albumin promotes atp-binding cassette transporter-dependent sterol uptake in yeast. | sterol uptake in fungi is a multistep process that involves interaction between external sterols and the cell wall, incorporation of sterol molecules into the plasma membrane, and subsequent integration into intracellular membranes for turnover. atp-binding cassette (abc) transporters have been implicated in sterol uptake, but key features of their activity remain to be elucidated. here, we apply fluorescent cholesterol (nbd-cholesterol) to monitor sterol uptake under anaerobic and aerobic condi ... | 2014 | 25331273 |
| simultaneous detection of four common oral candida species from blood samples by the fluorescence polarization assay. | the genus candida is both the commensal microbe and the opportunistic pathogen, containing approximately 200 species inhabiting in oral cavity of 53 % of the general population. candida species can cause the diseases from local mucosal infections to systemic mycoses, even life-threatening infections in immunocompromised individuals. the timely differentiation of candida species is important for the guidance of clinical medication. four common candida species in chinese population (candida albica ... | 2015 | 25308858 |
| oral administration of the broad-spectrum antibiofilm compound toremifene inhibits candida albicans and staphylococcus aureus biofilm formation in vivo. | we here report on the in vitro activity of toremifene to inhibit biofilm formation of different fungal and bacterial pathogens, including candida albicans, candida glabrata, candida dubliniensis, candida krusei, pseudomonas aeruginosa, staphylococcus aureus, and staphylococcus epidermidis. we validated the in vivo efficacy of orally administered toremifene against c. albicans and s. aureus biofilm formation in a rat subcutaneous catheter model. combined, our results demonstrate the potential of ... | 2014 | 25288093 |
| abdominal candidiasis is a hidden reservoir of echinocandin resistance. | fks mutant candida isolates were recovered from 24% (6/25) of abdominal candidiasis patients exposed to echinocandin. candida glabrata (29%) and candida albicans (14%) mutants were identified. multidrug-resistant bacteria were recovered from 83% of fks mutant infections. mutations were associated with prolonged echinocandin exposure (p = 0.01), breakthrough infections (p = 0.03), and therapeutic failures despite source control interventions (100%). abdominal candidiasis is a hidden reservoir for ... | 2014 | 25288081 |
| changes in genotype and fluconazole susceptibility of isolates from patients with candida glabrata in tunisia. | candida glabrata has emerged as an opportunistic pathogen of considerable importance in invasive and superficial infections. | 2014 | 25285365 |
| novel intein-containing dna specific primers for rapid identification of candida glabrata using real-time pcr assays. | candida glabrata is an opportunistic human pathogen known to cause systemic and vaginal candidiasis. rapid detection of candida glabrata is indispensable for appropriate selection of antifungal drugs for chemotherapy. the study describes a unique intein-containing dna fragment for specific detection of c. glabrata. the designed oligonucleotides detected c. glabrata (ct mean: 24.75 ± 1.1 and tm: 70.08 ± 0.23°c) in real-time pcr assays. the fluorescent signals were negative when the primers were t ... | 2014 | 25282343 |
| prevalence and risk factors of tinea capitis and tinea pedis in school children in turkey. | to evaluate the prevalence and risk factors of tinea capitis and tinea pedis in school children in turkey. | 2014 | 25272535 |
| candida nivariensis in comparison to different phenotypes of candida glabrata. | the purpose of the study was to establish the prevalence of new candida glabrata complex species: candida nivariensis and candida bracarensis isolated from clinical material, evaluate their phenotypes and the prevalence of gene family encoding extracellular glycosylphosphatidylinositol-linked aspartyl proteases, crucial for c. glabrata virulence. study material included 224 c. glabrata clinical strains. candida glabrata phenotypes were identified using chromagar candida medium. strains were anal ... | 2014 | 25267284 |
| echinocandin resistance, susceptibility testing and prophylaxis: implications for patient management. | this article addresses the emergence of echinocandin resistance among candida species, mechanisms of resistance, factors that promote resistance and confounding issues surrounding standard susceptibility testing. fungal infections remain a significant cause of global morbidity and mortality, especially among patients with underlying immunosupression. antifungal therapy is a critical component of patient management for acute and chronic diseases. yet, therapeutic choices are limited due to only a ... | 2014 | 25255923 |
| in vitro antimicrobial activity of an experimental dentifrice based on ricinus communis. | this study evaluated the antimicrobial activity of a ricinus communis-based experimental dentifrice for denture hygiene against the following standard strains: staphylococcus aureus, escherichia coli, streptococcus mutans, enterococcus faecalis, candida albicans and candida glabrata. the minimum inhibitory concentration (mic) assay was performed with r. communis in pure oil at 2.5%. only e. coli was not inhibited by r. communis, but the mic (0.0781%) was effective against the other microorganism ... | 2014 | 25252252 |
| membership and behavior of ultra-low-diversity pathogen communities present in the gut of humans during prolonged critical illness. | we analyzed the 16s rrna amplicon composition in fecal samples of selected patients during their prolonged stay in an intensive care unit (icu) and observed the emergence of ultra-low-diversity communities (1 to 4 bacterial taxa) in 30% of the patients. bacteria associated with the genera enterococcus and staphylococcus and the family enterobacteriaceae comprised the majority of these communities. the composition of cultured species from stool samples correlated to the 16s rrna analysis and addi ... | 2014 | 25249279 |
| β-glucan induces reactive oxygen species production in human neutrophils to improve the killing of candida albicans and candida glabrata isolates from vulvovaginal candidiasis. | vulvovaginal candidiasis (vvc) is among the most prevalent vaginal diseases. candida albicans is still the most prevalent species associated with this pathology, however, the prevalence of other candida species, such as c. glabrata, is increasing. the pathogenesis of these infections has been intensely studied, nevertheless, no consensus has been reached on the pathogenicity of vvc. in addition, inappropriate treatment or the presence of resistant strains can lead to rvvc (vulvovaginal candidias ... | 2014 | 25229476 |
| an essential role for phosphatidylinositol 3-kinase in the inhibition of phagosomal maturation, intracellular survival and virulence in candida glabrata. | the yeast class iii phosphoinositide 3-kinase (pi3k) that catalyses production of the lipid signalling molecule, phosphatidylinositol-3-phosphate, is primarily implicated in vesicle-mediated transport and autophagy. in this study, we identified, through a genetic screen, the candida glabrata cgvps15 gene, an orthologue of the saccharomyces cerevisiae pi3k regulatory subunit-encoding open reading frame (orf) to be required for impairment of phagosomal maturation in human macrophages. we also disr ... | 2015 | 25223215 |
| in vitro and in vivo removal of oral candida from the denture base. | to clarify the effectiveness of ultrasonic cleaning for removing candida lodged in the denture base. | 2016 | 25220495 |
| osteomyelitis caused by candida glabrata in the distal phalanx. | osteomyelitis caused by candida glabrata is rare and its optimal treatment is unknown. here we report a case of osteomyelitis caused by c. glabrata in the distal phalanx in a 54-year-old woman. despite partial resection of the nail and administering a 1-month course of antibiotics for paronychia, the local swelling remained and an osteolytic lesion was found. c. glabrata osteomyelitis of the distal phalanx was later diagnosed after curettage. thereafter, the patient was treated with antifungal a ... | 2014 | 25215255 |
| oral candida carriage and prevalence of candida species among maras powder users and non-users. | the aim of this study was to investigate candida carriage and species in maras powder users and non-users. | 2015 | 25213478 |
| eukaryote culturomics of the gut reveals new species. | the repertoire of microeukaryotes in the human gut has been poorly explored, mainly in individuals living in northern hemisphere countries. we further explored this repertoire using pcr-sequencing and culture in seven individuals living in four tropical countries. a total of 41 microeukaryotes including 38 different fungal species and three protists were detected. four fungal species, davidiella tassiana, davidiella sp., corticiaceae sp., and penicillium sp., were uniquely detected by culture; 2 ... | 2014 | 25210972 |
| identification of genomic binding sites for candida glabrata pdr1 transcription factor in wild-type and ρ0 cells. | the fungal pathogen candida glabrata is an emerging cause of candidiasis in part owing to its robust ability to acquire tolerance to the major clinical antifungal drug fluconazole. similar to the related species candida albicans, c. glabrata most typically gains azole tolerance via transcriptional induction of a suite of resistance genes, including a locus encoding an abcg-type atp-binding cassette (abc) transporter that is referred to as cdr1 in candida species. in c. glabrata, cdr1 expression ... | 2014 | 25199772 |
| metabolism in fungal pathogenesis. | fungal pathogens must assimilate local nutrients to establish an infection in their mammalian host. we focus on carbon, nitrogen, and micronutrient assimilation mechanisms, discussing how these influence host-fungus interactions during infection. we highlight several emerging trends based on the available data. first, the perturbation of carbon, nitrogen, or micronutrient assimilation attenuates fungal pathogenicity. second, the contrasting evolutionary pressures exerted on facultative versus ob ... | 2014 | 25190251 |
| the candida pathogenic species complex. | candida species are the most common causes of fungal infection. approximately 90% of infections are caused by five species: candida albicans, candida glabrata, candida tropicalis, candida parapsilosis, and candida krusei. three (c. albicans, c. tropicalis, and c. parapsilosis) belong to the ctg clade, in which the ctg codon is translated as serine and not leucine. c. albicans remains the most commonly isolated but is decreasing relative to the other species. the increasing incidence of c. glabra ... | 2014 | 25183855 |
| digestive fungal flora in asymptomatic subjects in bobo-dioulasso, burkina faso. | to identify candida species in asymptomatic subjects in bobo-dioulasso (burkina faso) by the matrix-assisted laser desorption ionization-time of flight mass spectrometry. | 2014 | 25183337 |
| [the use of caspofungin in extremely low birth weight preterm infants based on clinical trials and reports considering the own experience (case report)]. | in recent years, despite of significant progress in the treatment of premature infants with extremely low body weight, still a significant problem remains severe, treatment-resistant generalized infections among which the percentage of fungal infections is increasing. the invasive candidiasis, especially caused by non-albicans species of candida, are of particular importance. in the case of resistance on fluconazole and amphotericin b the use of echinocandin drug may be a useful choice. this pap ... | 2014 | 25182262 |
| contribution of the slt2-regulated transcription factors to echinocandin tolerance in candida glabrata. | echinocandin-class antifungals, including micafungin, are considered as the first-line treatment for candida glabrata infections. however, recent epidemiological surveys have revealed an increasing number of c. glabrata isolates exhibiting decreased echinocandin susceptibilities. the slt2 mitogen-activated protein kinase pathway is important for maintenance of cell wall integrity in fungi. rlm1 and swi4-swi6 cell cycle box binding factor (sbf) are transcription factors downstream of slt2. while ... | 2014 | 25175946 |
| host factors and clinical outcomes of candida colonization in critically ill patients. | this study aimed to describe factors and outcomes associated with candida colonization of critically ill patients. this was a cross-sectional study conducted over 2 weeks in the intensive care unit (icu) of a tertiary care hospital at the texas medical center, houston, tx. all candida samples were prospectively collected with demographic and clinical data collected retrospectively. we examined 48 patients, 32 (67%) were colonized with candida spp; 25 (52.1%) patients were isolated with candida a ... | 2015 | 25173925 |
| susceptibility of candida albicans and candida glabrata biofilms to silver nanoparticles in intermediate and mature development phases. | the aim of this study was to investigate the susceptibility of candida albicans and candida glabrata biofilm development, in their intermediate and maturation stages, to the influence of silver nanoparticles (sn). | 2015 | 25168655 |
| the production of reactive oxygen species is a universal action mechanism of amphotericin b against pathogenic yeasts and contributes to the fungicidal effect of this drug. | amphotericin b (amb) is an antifungal drug that binds to ergosterol and forms pores at the cell membrane, causing the loss of ions. in addition, amb induces the accumulation of reactive oxygen species (ros), and although these molecules have multiple deleterious effects on fungal cells, their specific role in the action mechanism of amb remains unknown. in this work, we studied the role of ros in the action mechanism of amb. we determined the intracellular induction of ros in 44 isolates of diff ... | 2014 | 25155595 |
| environmental azole fungicide, prochloraz, can induce cross-resistance to medical triazoles in candida glabrata. | acquisition of azole resistance by clinically relevant yeasts in nature may result in a significant, yet undetermined, impact in human health. the main goal of this study was to assess the development of cross-resistance between agricultural and clinical azoles by candida spp. an in vitro induction assay was performed, for a period of 90 days, with prochloraz (pcz) - an agricultural antifungal. afterward, the induced molecular resistance mechanisms were unveiled. mic value of pcz increased signi ... | 2014 | 25132632 |
| activity of echinocandins and triazoles against a contemporary (2012) worldwide collection of yeast and moulds collected from invasive infections. | in this study, 1717 fungal clinical isolates causing invasive fungal infections were evaluated against nine antifungal agents using clinical and laboratory standards institute (clsi) reference broth microdilution methods. the isolates comprised 1487 candida spp., 109 aspergillus spp., 86 non-candida yeasts (including 52 isolates of cryptococcus neoformans) and 35 rare moulds obtained during 2012 from 72 hospitals worldwide. echinocandin resistance among candida spp. was low, and resistance rates ... | 2014 | 25129315 |
| ten-year review of invasive candida infections in a tertiary care center in saudi arabia. | to review the epidemiology of invasive candida infections in a single center in saudi arabia over a subsequent 10-year period. | 2014 | 25129180 |
| direct visualization of fungal infection in brains from patients with alzheimer's disease. | recently, we have reported the presence of fungal infections in patients with alzheimer's disease (ad). accordingly, fungal proteins and dna were found in brain samples, demonstrating the existence of infection in the central nervous system. in the present work, we raised antibodies to specific fungal species and performed immunohistochemistry to directly visualize fungal components inside neurons from ad patients. mice infected with candida glabrata were initially used to assess whether yeast c ... | 2015 | 25125470 |
| candida glabrata endophthalmitis transmitted from graft to host after descemet stripping automated endothelial keratoplasty. | 2014 | 25124791 | |
| prevalence of candida nivariensis and candida bracarensis in vulvovaginal candidiasis. | candida nivariensis and candida bracarensis were isolated from patients with vulvovaginal candidiasis (vvc). candida nivariensis and candida bracarensis were found in presumptive candida glabrata isolates, which were identified using the api candida system. we retrospectively re-examined vaginal presumptive candida glabrata isolates for candida nivariensis and candida bracarensis from january 1, 2003, through december 31, 2012, via detection of the its1 region and the 5.8s ribosomal rna gene. am ... | 2014 | 25118875 |
| trends in antifungal susceptibility of candida species--one year observation. | in the past years opportunistic fungal infections have seriously increased, mainly in immunocompromised patients. the aim of the study was to determine the prevalence of yeast-like fungi in invasive candidiasis and to estimate its susceptibility to chosen antifungal agents. one hundred and sixty strains of yeast-like fungi were cultured from various clinical material: samples from lower respiratory tract, blood, the peritoneal cavity and others. the susceptibility tests were established accordin ... | 2014 | 25115116 |
| nosocomial bloodstream candida infections in a tertiary-care hospital in south brazil: a 4-year survey. | the aims of this study were to evaluate the epidemiology of nosocomial candidemia in a tertiary hospital in south brazil and the in vitro antifungal susceptibility of isolates. blood strains from 108 patients were identified by pcr-based method. some 30.5 % of candidemia were caused by candida tropicalis, 28.7 % were due to candida albicans, 24.1 % with candida parapsilosis sensu stricto, 8.3 % with candida glabrata sensu lato, 1.8 % involved candida krusei and 6.6 % with other species. candidem ... | 2014 | 25103140 |
| evaluation of blood culture media for the detection of fungi. | the aim of this study was to compare the utility of bactec™ mycosis-ic/f (mycosis), bactec™ plus aerobic/f (aerobic), and bactec™ plus anaerobic/f (anaerobic) media in the detection of fungi from simulated (obtained by the inoculation of tested media first with sterile sheep's blood and subsequently with one of 60 clinical yeast isolates) and clinical blood samples, taken during routine diagnostic examination in two hospitals. all tested strains grew on mycosis as well as aerobic bottles, and th ... | 2015 | 25098681 |
| isolation, selection and evaluation of yeasts for use in fermentation of coffee beans by the wet process. | during wet processing of coffee, the ripe cherries are pulped, then fermented and dried. this study reports an experimental approach for target identification and selection of indigenous coffee yeasts and their potential use as starter cultures during the fermentation step of wet processing. a total of 144 yeast isolates originating from spontaneously fermenting coffee beans were identified by molecular approaches and screened for their capacity to grow under coffee-associated stress conditions. ... | 2014 | 25087206 |
| longer incubation times for yeast fungemia: importance for presumptive treatment. | isolation rates of candida glabrata at ≤2 days were 8.9% and 34.8% at >2 days; for cryptococcus neoformans, they were 0.9% and 8.6%, respectively (1741 fungemia analyzed). an incubation time >2 days supports candins as presumptive treatment for c. glabrata, keeping in mind the risk of cryptococcus fungemia. | 2014 | 25085022 |
| prognostic factors and historical trends in the epidemiology of candidemia in critically ill patients: an analysis of five multicenter studies sequentially conducted over a 9-year period. | to describe temporal trends in the epidemiology, clinical management and outcome of candidemia in intensive care unit (icu) patients. | 2014 | 25082359 |
| molecular epidemiology of candida albicans and candida glabrata strains isolated from intensive care unit patients in poland. | over the last decades, candida spp have been responsible for an increasing number of infections, especially in patients requiring intensive care. knowledge of local epidemiology and analysis of the spread of these pathogens is important in understanding and controlling their transmission. the aim of this study was to evaluate the genetic diversity of 31 candida albicans and 17 candida glabrata isolates recovered from intensive care unit patients from the tertiary hospital in krakow between 2011- ... | 2014 | 25075783 |
| pivotal role for a tail subunit of the rna polymerase ii mediator complex cgmed2 in azole tolerance and adherence in candida glabrata. | antifungal therapy failure can be associated with increased resistance to the employed antifungal agents. candida glabrata, the second most common cause of invasive candidiasis, is intrinsically less susceptible to the azole class of antifungals and accounts for 15% of all candida bloodstream infections. here, we show that c. glabrata med2 (cgmed2), which codes for a tail subunit of the rna polymerase ii mediator complex, is required for resistance to azole antifungal drugs in c. glabrata. an in ... | 2014 | 25070095 |
| fumaric acid production by torulopsis glabrata: engineering the urea cycle and the purine nucleotide cycle. | a multi-vitamin auxotrophic torulopsis glabrata strain, a pyruvate producer, was further engineered to produce fumaric acid. using the genome-scale metabolic model inx804 of t. glabrata, four fumaric acid biosynthetic pathways, involving the four cytosolic enzymes, argininosuccinate lyase (asl), adenylosuccinate lyase (adsl), fumarylacetoacetase (faa), and fumarase (fum1), were found. athough single overexpression of each of the four enzymes in the cytosol improved fumaric acid production, the h ... | 2015 | 25060134 |
| synergistic antifungal effect of glabridin and fluconazole. | the incidence of invasive fungal infections is increasing in recent years. the present study mainly investigated glabridin (gla) alone and especially in combination with fluconazole (flc) against cryptococcus neoformans and candida species (candida albicans, candida tropicalis, candida krusei, candida parapsilosis and candida glabratas) by different methods. the minimal inhibitory concentration (mic) and the minimal fungicidal concentration (mfc) indicated that gla possessed a broad-spectrum ant ... | 2014 | 25058485 |
| how often do they have sex? a comparative analysis of the population structure of seven eukaryotic microbial pathogens. | the model of predominant clonal evolution (pce) proposed for micropathogens does not state that genetic exchange is totally absent, but rather, that it is too rare to break the prevalent pce pattern. however, the actual impact of this "residual" genetic exchange should be evaluated. multilocus sequence typing (mlst) is an excellent tool to explore the problem. here, we compared online available mlst datasets for seven eukaryotic microbial pathogens: trypanosoma cruzi, the fusarium solani complex ... | 2014 | 25054834 |
| time-kill assay and etest evaluation for synergy with polymyxin b and fluconazole against candida glabrata. | fluconazole-resistant candida glabrata is an emerging pathogen that causes fungemia. polymyxin b, a last-resort antibiotic used to treat multidrug-resistant gram-negative bacterial infections, has been found to possess in vitro fungicidal activity and showed synergy with fluconazole against a single strain of c. glabrata. since both agents may be used simultaneously in intensive care unit (icu) patients, this study was performed to test for possible synergy of this combination against 35 c. glab ... | 2014 | 25049251 |
| engineering the carbohydrate-binding site of epa1p from candida glabrata: generation of adhesin mutants with different carbohydrate specificity. | the n-terminal domain of the epa1p adhesin from candida glabrata (n-epa1p) is a calcium-dependent lectin, which confers the opportunistic yeast the ability to adhere to human epithelial cells. this lectin domain is able to interact with galactosides and, more precisely, with glycan molecules containing the galβ-1,3-galnac disaccharide, also known as the t-antigen. based on the crystallographic structure of the n-epa1p domain and the role of the variable loop cbl2 in glycan binding, saturation mu ... | 2014 | 25049238 |
| bpeaks: a bioinformatics tool to detect transcription factor binding sites from chipseq data in yeasts and other organisms with small genomes. | peak calling is a critical step in chipseq data analysis. choosing the correct algorithm as well as optimized parameters for a specific biological system is an essential task. in this article, we present an original peak-calling method (bpeaks) specifically designed to detect transcription factor (tf) binding sites in small eukaryotic genomes, such as in yeasts. as tf interactions with dna are strong and generate high binding signals, bpeaks uses simple parameters to compare the sequences (reads ... | 2014 | 25041923 |
| [fructose transporter in yeasts]. | study of hexoses transporter started with discovery of galactose permease in saccharomyces cerevisiae. glucose, fructose and mannose assimilation is assumed by numerous proteins encoded by different genes. to date over 20 hexoses transporters, belonging to sugar porter family and to major facilitator superfamily, were known. genome sequence analysis of candida glabrata, kluyveromyces lactis, yarrowia lipolytica, s. cerevisaie and debaryomyces hansenii reveled potential presence of 17-48 sugar po ... | 2014 | 25033548 |
| [case of candidemia with candida glabrata with confirmation of the acquisition of micafungin sensitivity due to new mutation of fks gene mutation]. | 2014 | 25033496 | |
| occurrence and characterization of candida nivariensis from a culture collection of candida glabrata clinical isolates in malaysia. | candida nivariensis and c. bracarensis have been recently identified as emerging yeast pathogens which are phenotypically indistinguishable from c. glabrata. however, there is little data on the prevalence and antifungal susceptibilities of these species. | 2014 | 25022264 |
| [evaluation of the vitek 2 system (ast-yso1 cards) for antifungal susceptibility testing against different candida species]. | the aim of this investigation was to evaluate the results of antifungal susceptibility for various candida species using the vitek 2 semi-automated system (ast-yso1 cards, biomérieux), and to compare them with those obtained by the clsi (clinical and laboratory standards institute) broth microdilution reference method (document m27-a3,2008). the essential agreement (ea) was > 90%, except for candida glabrata against voriconazole (vcz); and for candida krusei against fluconazole (fcz). the overal ... | 2014 | 25011594 |
| distribution of candida species isolated from blood cultures in hospitals in osaka, japan. | candida species are clinically important causes of bloodstream infections because their mortality is very high. given that some species of candida are azole-resistant, identifying the distributions of candida species could facilitate the formulation of an appropriate empirical antifungal therapy. it has been shown that the distribution varies depending on the continent, country, city, and hospital. in this paper, we describe the distributions of species in hospitals in northern osaka, japan. | 2014 | 25009091 |
| [system metabolic engineering strategies for 2,3-butandione production by torulopsis glabrata]. | we regulated the carbon flux distribution of torulopsis glabrata cctcc m202019, an efficient pyruvate-producing microorganism, for improved 2, 3-butandione production. | 2014 | 25007652 |
| differential response of candida albicans and candida glabrata to oxidative and nitrosative stresses. | invasive candidiasis is associated with high mortality in immunocompromised and hospitalized patients. candida albicans is the main pathological agent followed by candida glabrata, candida krusei, candida parapsilosis, and candida tropicalis. these pathogens colonize different host tissues in humans as they are able to neutralize the reactive species generated from nitrogen and oxygen during the respiratory burst. among the enzymatic mechanisms that candida species have developed to protect agai ... | 2014 | 25002360 |
| a systematic analysis reveals an essential role for high-affinity iron uptake system, haemolysin and cfem domain-containing protein in iron homoeostasis and virulence in candida glabrata. | iron is an essential nutrient for all living organisms and human pathogens employ a battery of factors to scavenge iron from the high-affinity iron-binding host proteins. in the present study, we have elucidated, via a candidate gene approach, major iron acquisition and homoeostatic mechanisms operational in an opportunistic human fungal pathogen candida glabrata. phenotypic, biochemical and molecular analysis of a set of 13 c. glabrata strains, deleted for proteins potentially implicated in iro ... | 2014 | 24987864 |
| essential oil composition and antimicrobial activities of two closely related species, alpinia mutica roxb. and alpinia latilabris ridl., from peninsular malaysia. | the essential oils obtained by hydrodistillation of the unripe and ripe fruits of alpinia mutica roxb. and alpinia latilabris ridl. were analysed by capillary gc and gc-ms. the oils were principally monoterpenic in nature. the unripe and ripe fruit oils of a. mutica were characterized by camphor (21.0% and 15.8%), camphene (16.6% and 10.2%), β-pinene (8.6% and 13.5%), and trans,trans-farnesol (8.0% and 11.2%), respectively. the oils of the unripe and ripe fruits were moderately active against st ... | 2014 | 24987733 |
| yeasts vectored by migratory birds collected in the mediterranean island of ustica and description of phaffomyces usticensis f.a. sp. nov., a new species related to the cactus ecoclade. | nine yeast species belonging to genera candida, cryptococcus, phaffomyces, rhodotorula and wickerhamomyces, and one species of aureobasidium genus were isolated from the cloaca of migratory birds. candida glabrata and c. inconspicua were the species most frequently isolated and wickerhamomyces sylviae, which has recently been described as a new species isolated from bird cloaca, was again found. the majority of isolates showed the ability to grow up to 40 °c and/or at ph 3.0, two environmental c ... | 2014 | 24981278 |
| [a case of micafungin-hyposensitive candida glabrata due to fks2 gene mutation]. | candida glabrata was continuously isolated in cultured urine samples from a subject with thrombotic thrombocytopenic purpura. yeast-like fungal phagocytosis found in gram staining led to agents being tested for antifungal susceptibility, revealing hyposensitivity to micafungin (mcfg) of mic < 2 mg/ml. mcfg administered for 10 days failed to cure c. glabrata infection. to clarify why hyposensitivity occurred, we analyzed the fks gene sequence using the pcr, finding a deficit of 3 bases coding phe ... | 2014 | 24979950 |
| development of multiplex loop-mediated isothermal amplification assays to detect medically important yeasts in dairy products. | rapid detection of yeast contamination is important in the food industry. we have developed loop-mediated isothermal amplification (lamp) assays to detect the emerging opportunistic pathogenic yeasts: candida albicans, candida glabrata, candida tropicalis, the candida parapsilosis group, trichosporon asahii, and trichosporon mucoides. these yeasts may cause deep-seated candidiasis or trichosporonosis. four lamp primer sets specific for candida were designed to target the internal transcribed spa ... | 2014 | 24965944 |
| isavuconazole and nine comparator antifungal susceptibility profiles for common and uncommon candida species collected in 2012: application of new clsi clinical breakpoints and epidemiological cutoff values. | the in vitro activity of isavuconazole and nine antifungal comparator agents was assessed using reference broth microdilution methods against 1,421 common and uncommon species of candida from a 2012 global survey. isolates were identified using chromagar, biochemical methods and sequencing of its and/or 28s regions. candida spp. were classified as either susceptible or resistant and as wild type (wt) or non-wt using clsi clinical breakpoints or epidemiological cutoff values, respectively, for th ... | 2014 | 24952015 |
| silver colloidal nanoparticle stability: influence on candida biofilms formed on denture acrylic. | our aim in this study was to evaluate how the chemical stability of silver nanoparticles (sns) influences their efficacy against candida albicans and c. glabrata biofilms. several parameters of sn stability were tested, namely, temperature (50ºc, 70ºc, and 100ºc), ph (5.0 and 9.0), and time of contact (5 h and 24 h) with biofilms. the control was defined as sns without temperature treatment, ph 7, and 24 h of contact. these colloidal suspensions at 54 mg/l were used to treat mature candida biofi ... | 2014 | 24951722 |
| yeast diversity associated to sediments and water from two colombian artificial lakes. | in colombia, knowledge of the yeast and yeast-like fungi community is limited because most studies have focused on species with clinical importance. sediments and water represent important habitats for the study of yeast diversity, especially for yeast species with industrial, biotechnological, and bioremediation potential. the main purpose of this study was to identify and compare the diversity of yeast species associated with sediment and water samples from two artificial lakes in universidad ... | 2014 | 24948924 |
| systematic phenotyping of a large-scale candida glabrata deletion collection reveals novel antifungal tolerance genes. | the opportunistic fungal pathogen candida glabrata is a frequent cause of candidiasis, causing infections ranging from superficial to life-threatening disseminated disease. the inherent tolerance of c. glabrata to azole drugs makes this pathogen a serious clinical threat. to identify novel genes implicated in antifungal drug tolerance, we have constructed a large-scale c. glabrata deletion library consisting of 619 unique, individually bar-coded mutant strains, each lacking one specific gene, al ... | 2014 | 24945925 |
| evaluation of possibilities in identification and susceptibility testing for candida glabrata clinical isolates with the integral system yeast plus (isyp). | the aim of this study was to evaluate possibilities of correct identification and susceptibility testing of c. glabrata clinical isolates with integral system yeast plus (isyp). for species identification, as the reference method, api candida test and species-specific pcr reactions were used. the potential of antifungal susceptibility testing by the isyp test was compared with the sensititre yeast one. whilst the reference methods confirmed that the received population (n = 65 isolates) represen ... | 2014 | 24939684 |
| a case of candida glabrata severe urinary sepsis successfully treated with micafungin. | candida glabrata is frequently resistant to fluconazole, and in advanced renal failure the safe use of this and other recommended drugs is limited. we report a case of a 56 years-old diabetic woman with renal failure and severe urinary sepsis from c. glabrata successfully treated with micafungin. | 2014 | 24936401 |
| pseudomonas aeruginosa inhibits the growth of pathogenic fungi: in vitro and in vivo studies. | the aim of the present study was to investigate the inhibitory effect of pseudomonas aeruginosa (pa) on pathogenic fungi, including candida albicans (ca), candida tropicalis (ct), candida glabrata (cg), candida parapsilosis (cp) and candida krusei (ck), in vitro and in vivo. in total, 24 pa strains were collected from clinical specimens and identified by gram staining, oxidase production and the api 20ne system. cross-streak, disk diffusion and co-culture methods were used to observe the inhibit ... | 2014 | 24926335 |
| candida albicans promotes invasion and colonisation of candida glabrata in a reconstituted human vaginal epithelium. | the principal aim of this study was to investigate the in vitro co-infection of a reconstituted human vaginal epithelium (rhve) by candida albicans and candida glabrata. | 2014 | 24924556 |
| systemic candidiasis in extremely low birthweight (elbw) neonates despite the routine use of topical miconazole prophylaxis: trends, risk factors and outcomes over an 11-year period. | this study aims to determine the incidence, trends of systemic candidiasis and meningitis in extremely low birthweight (elbw) neonates (<1000 gms) despite the routine use of topical miconazole prophylaxis and to compare the risk factors, adverse outcomes and comorbidities with controls. | 2014 | 24919490 |
| fluconazole affects the alkali-metal-cation homeostasis and susceptibility to cationic toxic compounds of candida glabrata. | candida glabrata is a salt-tolerant and fluconazole (flc)-resistant yeast species. here, we analyse the contribution of plasma-membrane alkali-metal-cation exporters, a cation/proton antiporter and a cation atpase to cation homeostasis and the maintenance of membrane potential (δψ). using a series of single and double mutants lacking cnh1 and/or ena1 genes we show that the inability to export potassium and toxic alkali-metal cations leads to a slight hyperpolarization of the plasma membrane of c ... | 2014 | 24913686 |
| in vitro evaluation of the enzymatic activity profile of non-albicans candida species isolated from patients with oral candidiasis with or without diabetes. | to evaluate the expression of phospholipase (pl) and secreted aspartyl proteinase (sap) by candida glabrata and c tropicalis obtained from the denture biofilms of healthy participants (16 isolates), patients with oral candidiasis with diabetes (10 isolates), and patients with oral candidiasis without diabetes (25 isolates). | 2014 | 24908598 |
| time to positivity and detection of growth in anaerobic blood culture vials predict the presence of candida glabrata in candidemia: a two-center european cohort study. | this study shows the accuracy of exclusive or earlier growth in anaerobic vials to predict candida glabrata in a large series of candidemic patients from two european hospitals using the bactec 9240 system. alternatively, c. glabrata can be predicted by a time to positivity cutoff value, which should be determined for each setting. | 2014 | 24899027 |
| evaluation of multiplex pcr in first episodes of febrile neutropenia as a tool to improve early yeast diagnosis in leukemic/preleukemic patients. | in febrile neutropenic onco-hematological patients, delayed microbiological diagnosis leads to an increase in morbidity and mortality. identification of the microorganism changes antibiotic therapy in more than half of cases; however, in only 20-30 % of such cases pathogen isolation is achieved. this study evaluates the frequency of fungus infection and its etiology in onco-hematological patients with febrile neutropenia utilizing blood cultures and non-commercial multiplex polymerase chain reac ... | 2014 | 24897963 |
| role of fks mutations in candida glabrata: mic values, echinocandin resistance, and multidrug resistance. | candida glabrata is the second leading cause of candidemia in u.s. hospitals. current guidelines suggest that an echinocandin be used as the primary therapy for the treatment of c. glabrata disease due to the high rate of resistance to fluconazole. recent case reports indicate that c. glabrata resistance to echinocandins may be increasing. we performed susceptibility testing on 1,380 isolates of c. glabrata collected between 2008 and 2013 from four u.s. cities, atlanta, baltimore, knoxville, and ... | 2014 | 24890592 |
| fks mutant candida glabrata: risk factors and outcomes in patients with candidemia. | echinocandins are recommended for candia glabrata candidemia. mutations in the fks1 and fks2 genes are associated with echinocandin resistance. few studies have assessed risk factors for fks mutant isolates and outcomes in patients receiving micafungin treatment. | 2014 | 24879785 |
| nosocomial candiduria in chronic liver disease patients at a hepatobilliary center. | nosocomial urinary tract infections (utis) are common in catheterized patients. fungal uti has become an important nosocomial problem over the past decade. the microbiology of candiduria is rapidly evolving and new trends are being reported. | 2014 | 24872653 |
| upc2a is required for high-level azole antifungal resistance in candida glabrata. | candida glabrata, the second most common cause of candida infections, is associated with high rates of mortality and often exhibits resistance to the azole class of antifungal agents. upc2 and ecm22 in saccharomyces cerevisiae and upc2 in candida albicans are the transcriptional regulators of erg11, the gene encoding the target of azoles in the ergosterol biosynthesis pathway. recently two homologs for these transcription factors, upc2a and upc2b, were identified in c. glabrata. one of these, up ... | 2014 | 24867980 |
| antifungal resistance to fluconazole and echinocandins is not emerging in yeast isolates causing fungemia in a spanish tertiary care center. | accurate knowledge of fungemia epidemiology requires identification of strains to the molecular level. various studies have shown that the rate of resistance to fluconazole ranges from 2.5% to 9% in candida spp. isolated from blood samples. however, trends in antifungal resistance have received little attention and have been studied only using clsi m27-a3 methodology. we assessed the fungemia epidemiology in a large tertiary care institution in madrid, spain, by identifying isolates to the molec ... | 2014 | 24867979 |
| breakthrough invasive fungal disease in patients receiving posaconazole primary prophylaxis: a 4-year study. | posaconazole (psc) is currently recommended as primary prophylaxis in neutropenic patients with acute myeloid leukaemia (aml) and in allogenic haematopoietic stem cell transplantation (ahsct) recipients with graft-versus-host disease (gvhd). studies focusing on breakthrough invasive fungal disease (ifd) upon psc prophylaxis show disparate results. in order to evaluate the incidence of ifd in patients on psc prophylaxis and identify ifd risk factors, we carried out a retrospective study of all co ... | 2014 | 24861577 |
| candida glabrata: a deadly companion? | the yeast candida glabrata has become a major fungal opportunistic pathogen of humans since the 1980s. contrary to what its name suggests, it is much closer, phylogenetically, to the model yeast saccharomyces cerevisiae than to the most prevalent human fungal pathogen, candida albicans. its similarity to s. cerevisiae fortunately extends to their amenability to molecular genetics methods. c. glabrata is now described as part of the nakaseomyces clade, which includes two new pathogens and other e ... | 2014 | 24861573 |
| microbiocidal effects of various taurolidine containing catheter lock solutions. | we have recently shown that a catheter lock solution containing taurolidine dramatically decreases catheter-related bloodstream infections (crbsi) in patients on home parenteral nutrition (hpn) when compared to heparin. since several taurolidine formulations are commercially available, some of which also contain citrate or heparin, we were interested in the effect of these different locks on growth and biofilm formation of fungal, gram-negative and gram-positive pathogens that are known to imped ... | 2015 | 24861410 |
| in vitro antifungal activities of leaf extracts of lippia alba (verbenaceae) against clinically important yeast species. | there are few studies reporting the antifungal activities of lippia alba extracts. | 2014 | 24861304 |
| milbemycin a4 oxime as a probe of azole transport in candida glabrata. | azole resistance in candida glabrata, a pathogenic yeast, has prompted studies of compounds that have therapeutic potential by reversing azole resistance. milbemycin a4 oxime blocked azole efflux and enhanced azole susceptibility fourfold in 28 clinical isolates of c. glabrata. specificity of the milbemycin a4 oxime effect depended on the drug transporter and the substrate being effluxed. the major effect of milbemycin a4 oxime was inhibition of azole and rhodamine 6g efflux by the atp-binding c ... | 2014 | 24838041 |
| polymerase chain reaction melting profiles of candida glabrata clinical isolates in solid organ transplant recipients in comparison to the other group of surgical patients. | the aim of the retrospective study were to estimate the prevalence of candida glabrata in liver and kidney transplant recipients compared to patients with short bowel syndrome receiving chronic total parenteral nutrition and relevance of the polymerase chain reaction melting profile (pcr mp) method for candida glabrata strains differentiation. c. glabrata clinical strains isolated from patients were identified by using standard mycological procedures. the analysis of genetic relatedness of the i ... | 2014 | 24836833 |
| [study on candida infections in intensive care unit from 2008 to 2012]. | to investigate and analyze the candida infection situation at the intensive care unit (icu) in tianjin fourth central hospital from 2008 to 2012. | 2014 | 24831637 |
| set of classical pcrs for detection of mutations in candida glabrata fks genes linked with echinocandin resistance. | clinical echinocandin resistance among candida glabrata strains is increasing, especially in the united states. antifungal susceptibility testing is considered mandatory to guide therapeutic decisions. however, these methodologies are not routinely performed in the hospital setting due to their complexity and the time needed to obtain reliable results. echinocandin failure in c. glabrata is linked exclusively to fks1p and fks2p amino acid substitutions, and detection of such substitutions would ... | 2014 | 24829248 |
| diagnostic usefulness of differential time to positivity for catheter-related candidemia. | a differential time to positivity (dtp) of ≥ 120 min is useful for diagnosing catheter-related bacteremia, but data on diagnosing catheter-related candidemia (crc) in this way are limited. we wished to evaluate the usefulness of the dtp for diagnosing crc. all adult patients who had the same candida species isolated from blood cultures drawn simultaneously from a central venous catheter (cvc) and a peripheral vein were included at a tertiary care hospital over an 18-month period. a total of 105 ... | 2014 | 24829236 |
| periocular and anterior orbital necrosis after upper eyelid gold weight loading: operation-related or self-inflicted? | a 44-year-old woman, who had undergone gold-weight implantation due to facial palsy and lagophthalmos, arrived at the ophthalmology ward with eyelid swelling and erythema, which rapidly deteriorated under intravenous antibiotics to a necrotic process involving the periocular tissues, the eye, and the anterior orbit. despite prompt removal of the gold weight, the patient's ocular and systemic condition continued to deteriorate, necessitating evisceration and debridement of necrotic tissue. cultur ... | 2014 | 24812491 |
| yeast oropharyngeal colonization in human immunodeficiency virus-infected patients in central taiwan. | a prospective, cross-sectional study was conducted at a medical center in central taiwan to understand the prevalence, associated factors, and microbiologic features for oropharyngeal yeast colonization in human immunodeficiency virus-infected outpatients. oral yeast colonization was detected in 127 (45 %) patients, including 21 (16.5 %) colonized by more than one species. of the 154 isolates, candida albicans was the most common species (114, 74 %), followed by candida dubliniensis (10, 6.5 %), ... | 2014 | 24804977 |
| candida glabrata intra-abdominal candidiasis is characterized by persistence within the peritoneal cavity and abscesses. | the pathogenesis of candida glabrata infections is poorly understood. we studied the pathogenesis of intra-abdominal candidiasis (iac) in mice that were infected intraperitoneally with c. glabrata and sterile feces. c. glabrata bg2 (5 × 10(8) cfu) caused a 100% mortality rate. sublethal inocula of bg2 (1 × 10(8) or 1 × 10(7) cfu) caused peritonitis that progressed to abscesses. three clinical c. glabrata strains (5 × 10(8) cfu) caused 80 to 100% mortality rates, while a fourth (strain 346) cause ... | 2014 | 24799629 |
| efficacy and safety of antifungal additives in optisol-gs corneal storage medium. | optisol-gs, the most common corneal storage medium in the united states, contains antibacterial but no antifungal supplementation. most postkeratoplasty endophthalmitis and keratitis cases are now of a fungal origin. | 2014 | 24789459 |
| identification of candida glabrata genes involved in ph modulation and modification of the phagosomal environment in macrophages. | candida glabrata currently ranks as the second most frequent cause of invasive candidiasis. our previous work has shown that c. glabrata is adapted to intracellular survival in macrophages and replicates within non-acidified late endosomal-stage phagosomes. in contrast, heat killed yeasts are found in acidified matured phagosomes. in the present study, we aimed at elucidating the processes leading to inhibition of phagosome acidification and maturation. we show that phagosomes containing viable ... | 2014 | 24789333 |
| breakthrough invasive candida glabrata in patients on micafungin: a novel fks gene conversion correlated with sequential elevation of mic. | candida glabrata strains sequentially isolated from blood developed resistance to micafungin (mics from <0.015 to 4 μg/ml). a novel mutation identified in micafungin-resistant strains at bp 262 of fks2 (containing a deletion of f659 [f659del]) was inserted into the homologous region in fks1. | 2014 | 24789192 |
| recurrent arthritis by candida glabrata, a diagnostic and therapeutic challenge. | infectious arthritis due to candida glabrata is very rare. a 40-year-old iranian man had developed a painful swelling on the left knee since a year ago. a surgery (meniscectomy) was performed on his knee. however, in follow-up visit after 2 months, the patient's condition was deteriorated. direct examination of synovial fluid with gram and hematoxylin-eosin stains were negative for any bacterial or fungal infection or crystal elements; however, inoculation into bactec™ mycosis ic/f and plus aero ... | 2014 | 24752453 |