Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| fibrolytic enzyme and ammonia application effects on the nutritive value, intake, and digestion kinetics of bermudagrass hay in beef cattle. | the objectives were to compare the effect of exogenous fibrolytic enzyme (biocellulase a20) or anhydrous ammonia (4% dm) treatment on the nutritive value, voluntary intake, and digestion kinetics of bermudagrass (cynodon dactylon cultivar coastal) hay harvested after 2 maturities (5- and 13-wk regrowths). six individually housed, ruminally cannulated brangus steers (bw 325 ± 10 kg) were used in an experiment with a 6 × 6 latin square design with a 3 (additives) × 2 (maturities) factorial arrange ... | 2013 | 23893992 |
| two β-xylanases from aspergillus terreus: characterization and influence of phenolic compounds on xylanase activity. | sugarcane bagasse was used as an inexpensive alternative carbon source for production of β-xylanases from aspergillus terreus. the induction profile showed that the xylanase activity was detected from the 6th day of cultivation period. two low molecular weight enzymes, named xyl t1 and xyl t2 were purified to apparent homogeneity by ultrafiltration, gel filtration and ion exchange chromatographies and presented molecular masses of 24.3and 23.60 kda, as determined by sds-page, respectively. xyl t ... | 2013 | 23892064 |
| the influence of aspergillus niger transcription factors arar and xlnr in the gene expression during growth in d-xylose, l-arabinose and steam-exploded sugarcane bagasse. | the interest in the conversion of plant biomass to renewable fuels such as bioethanol has led to an increased investigation into the processes regulating biomass saccharification. the filamentous fungus aspergillus niger is an important microorganism capable of producing a wide variety of plant biomass degrading enzymes. in a. niger the transcriptional activator xlnr and its close homolog, arar, controls the main (hemi-)cellulolytic system responsible for plant polysaccharide degradation. sugarc ... | 2013 | 23892063 |
| pseudozyma vetiver sp. nov., a novel anamorphic ustilaginomycetous yeast species isolated from the phylloplane in thailand. | three strains representing one novel yeast species were isolated from the phylloplanes of the vetiver grasses (dmku-lv90 and dmku-lv99(t)) and sugarcane (dmku-sp260) collected in thailand by leaf washing followed by a plating technique. on the basis of morphological, biochemical, physiological and chemotaxonomic characteristics and the sequence analysis of the d1/d2 region of the large subunit (lsu) rrna gene and the internal transcribed spacer region (its), the three strains were found to repre ... | 2013 | 23877892 |
| differential regulation of defense-related gene expression in response to red rot pathogen colletotrichum falcatum infection in sugarcane. | red rot is a serious disease of sugarcane caused by the fungus colletotrichum falcatum imposing a considerable economic loss annually in all sugarcane-producing countries. in this study, we analyzed the early resistance response of sugarcane to red rot fungus by comparing the differences between control and inoculated stalk tissues. differential display reverse transcription polymerase chain reaction (dd-rt-pcr) was employed to identify altered expression of genes in disease-resistant cv co 9300 ... | 2013 | 23861092 |
| sugarcane bagasse hydrolysis using yeast cellulolytic enzymes. | ethanol fuel production from lignocellulosic biomass is emerging as one of the most important technologies for sustainable development. to use this biomass, it is necessary to circumvent the physical and chemical barriers presented by the cohesive combination of the main biomass components, which hinders the hydrolysis of cellulose and hemicellulose into fermentable sugars. this study evaluated the hydrolytic capacity of enzymes produced by yeasts, isolated from the soils of the brazilian cerrad ... | 2013 | 23851270 |
| an-overview on invertase in sugarcane. | saccharum officinarum is one of the most cultivated hybrid varieties among the sugarcane varieties. in sugarcane plant sucrose is the major carbohydrate which can be stored and transported. different physiological and biochemical studies on this crop report that invertase activity and sucrose concentration some how are key limiting step in the process of sucrose accumulation. significant efforts have been made in relation to the sucrose cycle by altering the sucrose phosphate synthetase, sucrose ... | 2013 | 23847400 |
| molecular cloning and characterization of two pathogenesis-related β-1,3-glucanase genes scglua1 and scglud1 from sugarcane infected by sporisorium scitamineum. | two β-1,3-glucanase genes from sugarcane were cloned and characterized. they were all located in apoplast and involves in different expression patterns in biotic and abiotic stress. smut caused by sporisorium scitamineum is a serious disease in the sugarcane industry. β-1,3-glucanase, a typical pathogenesis-related protein, has been shown to express during plant-pathogen interaction and involves in sugarcane defense response. in this study, β-1,3-glucanase enzyme activity in the resistant variet ... | 2013 | 23842883 |
| production of bioethanol from fermented sugars of sugarcane bagasse produced by lignocellulolytic enzymes of exiguobacterium sp. vsg-1. | exiguobacterium sp. vsg-1 was isolated from the soil sample and characterized for the production of lignocellulolytic enzymes. production of these enzymes by the strain vsg-1 was carried out using steam-exploded sugarcane bagasse (scb) and found to secrete cellulase, pectinase, mannanase, xylanase, and tannase. the growth and enzyme production were found to be optimum at ph 9.0 and 37 °c. upon steam explosion of scb, the cellulose increased by 42 %, whereas hemicelluloses and lignin decreased by ... | 2013 | 23832861 |
| effect of silicon soil amendment on performance of sugarcane borer, diatraea saccharalis (lepidoptera: crambidae) on rice. | the sugarcane borer, diatraea saccharalis (f.), is a pest of graminaceous crops in the southern usa, including sugarcane, maize, and rice. this study was conducted to investigate the effect of silicon (si) soil amendments on performance of sugarcane borer, d. saccharalis, on two rice cultivars, cocodrie and xl723. there was a significant increase in the si content of rice plants supplemented with calcium silicate as compared to non-treated plants. soil si amendment led to lower relative growth r ... | 2013 | 23830057 |
| repetitive succinic acid production from lignocellulose hydrolysates by enhancement of atp supply in metabolically engineered escherichia coli. | in this study, repetitive production of succinic acid from lignocellulose hydrolysates by enhancement of atp supply in metabolically engineered e. coli is reported. escherichia coli ba305, a pflb, ldha, ppc, and ptsg deletion strain overexpressing atp-forming phosphoenolpyruvate (pep) carboxykinase (pepck), produced a final succinic acid concentration of 83 g l(-1) with a high yield of 0.87 g g(-1) total sugar in 36 h of three repetitive fermentations of sugarcane bagasse hydrolysate. furthermor ... | 2013 | 23819977 |
| comparative feedstock analysis in setaria viridis l. as a model for c4 bioenergy grasses and panicoid crop species. | second generation feedstocks for bioethanol will likely include a sizable proportion of perennial c4 grasses, principally in the panicoideae clade. the panicoideae contain agronomically important annual grasses including zea mays l. (maize), sorghum bicolor (l.) moench (sorghum), and saccharum officinarum l. (sugar cane) as well as promising second generation perennial feedstocks including miscanthus×giganteus and panicum virgatum l. (switchgrass). the underlying complexity of these polyploid gr ... | 2013 | 23802002 |
| enhanced transgene expression in sugarcane by co-expression of virus-encoded rna silencing suppressors. | post-transcriptional gene silencing is commonly observed in polyploid species and often poses a major limitation to plant improvement via biotechnology. five plant viral suppressors of rna silencing were evaluated for their ability to counteract gene silencing and enhance the expression of the enhanced yellow fluorescent protein (eyfp) or the β-glucuronidase (gus) reporter gene in sugarcane, a major sugar and biomass producing polyploid. functionality of these suppressors was first verified in n ... | 2013 | 23799071 |
| carbon partitioning in sugarcane (saccharum species). | focus has centered on c-partitioning in stems of sugarcane (saccharum sp.) due to their high-sucrose accumulation features, relevance to other grasses, and rising economic value. here we review how sugarcane balances between sucrose storage, respiration, and cell wall biosynthesis. the specific topics involve (1) accumulation of exceptionally high sucrose levels (up to over 500 mm), (2) a potential, turgor-sensitive system for partitioning sucrose between storage inside (cytosol and vacuole) and ... | 2013 | 23785381 |
| scmt2-1-3, a metallothionein gene of sugarcane, plays an important role in the regulation of heavy metal tolerance/accumulation. | plant metallothioneins (mts), which are cysteine-rich, low-molecular-weight, and metal-binding proteins, play important roles in detoxification, metal ion homeostasis, and metal transport adjustment. in this study, a novel metallothionein gene, designated as scmt2-1-3 (genbank accession number jq627644), was identified from sugarcane. scmt2-1-3 was 700 bp long, including a 240 bp open reading frame (orf) encoding 79 amino acid residues. a his-tagged scmt2-1-3 protein was successfully expressed i ... | 2013 | 23781509 |
| metaproteomic analysis of ratoon sugarcane rhizospheric soil. | the current study was undertaken to elucidate the mechanism of yield decline in ratoon sugarcane using soil metaproteomics combined with community level physiological profiles (clpp) analysis. | 2013 | 23773576 |
| comparative proteomic analysis of the plant-virus interaction in resistant and susceptible ecotypes of maize infected with sugarcane mosaic virus. | sugarcane mosaic virus (scmv) is an important viral pathogen and has caused serious losses in grain and forage yield. to identify candidate scmv resistance proteins and to explore the molecular mechanisms involved in the plant-scmv interaction, we conducted proteomic analyses of leaf samples from resistant and susceptible ecotypes of maize infected with scmv. proteins were analyzed by quantitative two-dimensional differential gel electrophoresis (2d-dige), and 93 protein spots showed statistical ... | 2013 | 23770298 |
| development and characterization of microsatellite loci for genetic studies of the sugarcane borer, diatraea saccharalis (lepidoptera: crambidae). | we present polymorphic microsatellite markers isolated for genetic studies of the sugarcane borer, diatraea saccharalis (fabricius). we isolated 16 microsatellite loci through an enriched genomic library protocol. after characterization, 12 markers showed polymorphic information expressed in the observed number of alleles (ranging from 2 to 7; 5 on average) and in the polymorphism information content (ranging from 0.292 to 0.771; 0.535 on average). these markers can be used in further studies to ... | 2013 | 23765969 |
| experimental investigation of microbiologically influenced corrosion of selected steels in sugarcane juice environment. | in the current study, ferritic stainless grades aisi 439 and aisi 444 were investigated as possible construction materials for machinery and equipment in the cane-sugar industry. their performance in corrosive cane-sugar juice environment was compared with the presently used low carbon steel aisi 1010 and austenitic stainless steel aisi 304. the tafel plot electrochemical technique was used to evaluate general corrosion performance. microbiologically influenced corrosion (mic) behaviour in sugar ... | 2013 | 23764955 |
| bacilliform dna-containing plant viruses in the tropics: commonalities within a genetically diverse group. | plant viruses, possessing a bacilliform shape and containing double-stranded dna, are emerging as important pathogens in a number of agricultural and horticultural crops in the tropics. they have been reported from a large number of countries in african and asian continents, as well as from islands from the pacific region. the viruses, belonging to two genera, badnavirus and tungrovirus, within the family caulimoviridae, have genomes displaying a common plan, yet are highly variable, sometimes e ... | 2013 | 23763585 |
| predominant nifh transcript phylotypes related to rhizobium rosettiformans in field-grown sugarcane plants and in norway spruce. | although some sugarcane cultivars may benefit substantially from biological nitrogen fixation (bnf), the responsible bacteria have been not identified yet. here, we examined the active diazotrophic bacterial community in sugarcane roots from africa and america by reverse transcription (rt)-pcr using broad-range nifh-specific primers. denaturing gradient gel electrophoresis (dgge) profiles obtained from sugarcane showed a low diversity at all sample locations with one phylotype amounting up to 10 ... | 2011 | 23761284 |
| effect of forced aeration on citric acid production by aspergillus sp. mutants in ssf. | citric acid (ca) is one of the most important products of fermentation in the world. a great variety of agro-industrial residues can be used in solid state fermentation. aspergillus niger parental strain (cct 7716) and two strains obtained by mutagenesis (cct 7717 and cct 7718) were evaluated in erlenmeyer flasks and glass columns using citric pulp (cp) as substrate/support, sugarcane molasses and methanol. best results using glass columns (forced aeration) were found in the fourth day of fermen ... | 2013 | 23760557 |
| xanthomonas albilineans ompa1 appears to be functionally modular and both the omc and c-like domains are necessary for leaf scald disease of sugarcane. | several ez-tn5 insertions in gene locus xalc_0557 (ompa1) of the sugarcane leaf scald pathogen xanthomonas albilineans xafl07-1 were previously found to strongly affect pathogenicity and endophytic stalk colonization. xalc_0557 has a predicted ompa n-terminal outer membrane channel (omc) domain and an ompa c-like domain. further analysis of mutant m468, with an ez-tn5 insertion in the upstream omc domain coding region, revealed impaired epiphytic and endophytic leaf survival, impaired resistance ... | 2013 | 23758144 |
| rim15p-mediated regulation of sucrose utilization during molasses fermentation using saccharomyces cerevisiae strain pe-2. | inherited loss-of-function mutations in the rim15p-mediated stress-response pathway contribute to the high fermentation rate of sake yeast strains. in the present study, we found that disruption of the rim15 gene in ethanol-producing saccharomyces cerevisiae strain pe-2 accelerated molasses fermentation through enhanced sucrose utilization following glucose starvation. | 2013 | 23757382 |
| agrobacterium tumefaciens-mediated in planta seed transformation strategy in sugarcane. | an efficient, reproducible and genotype-independent in planta transformation has been standardized for sugarcane using seed as explant. transgenic sugarcane production through agrobacterium infection followed by in vitro regeneration is a time-consuming process and highly genotype dependent. to obtain more number of transformed sugarcane plants in a relatively short duration, sugarcane seeds were infected with agrobacterium tumefaciens eha 105 harboring pcambia 1304-bar and transformed plants we ... | 2013 | 23749098 |
| utilization of pentoses from sugarcane biomass: techno-economics of biogas vs. butanol production. | this paper presents the techno-economics of greenfield projects of an integrated first and second-generation sugarcane biorefinery in which pentose sugars obtained from sugarcane biomass are used either for biogas (consumed internally in the power boiler) or n-butanol production via the abe batch fermentation process. the complete sugarcane biorefinery was simulated using aspen plus®. although the pentoses stream available in the sugarcane biorefinery gives room for a relatively small biobutanol ... | 2013 | 23748087 |
| the influence of total solids content and initial ph on batch biohydrogen production by solid substrate fermentation of agroindustrial wastes. | hydrogen is a valuable clean energy source, and its production by biological processes is attractive and environmentally sound and friendly. in méxico 5 million tons/yr of agroindustrial wastes are generated; these residues are rich in fermentable organic matter that can be used for hydrogen production. on the other hand, batch, intermittently vented, solid substrate fermentation of organic waste has attracted interest in the last 10 years. thus the objective of our work was to determine the eff ... | 2013 | 23732191 |
| purification and kinetics study of thermostable cellulase free xylanase from bacillus subtilis. | present study dealt with the production, purification and characterization of xylanase from bacillus subtilis- bs05 grown in submerged fermentation at 37(o)c for 48h using sugarcane bagasse as a substrate. xylanase enzyme was purified to homogeneity by ammonium sulphate fractionation followed by sephadex g-100 chromatography. the molecular mass of xylanase enzyme was found to be 23kda by sds-page. the optimum ph and temperature of xylanase enzyme was 5 and 50°c with stability range of 5-6 and 30 ... | 2013 | 23713898 |
| development of loop-mediated isothermal amplification for detection of leifsonia xyli subsp. xyli in sugarcane. | ratoon stunt, caused by the xylem-limited coryneform bacterium leifsonia xyli subsp. xyli (lxx), is a deep bacteriosis and prevalent in most of sugarcane-producing countries. based on loop-mediated isothermal amplification (lamp), we developed a method for detecting lxx. the major advantages of the lamp method are visual judgment by color and time saving with only 60 min for identification of lxx and without the need for costly pcr apparatus and gel scanner. in the present study, positive and ne ... | 2013 | 23710444 |
| design and optimization of ethanol production from bagasse pith hydrolysate by a thermotolerant yeast kluyveromyces sp. iipe453 using response surface methodology. | ethanol production from sugarcane bagasse pith hydrolysate by thermotolerant yeast kluyveromyces sp. iipe453 was analyzed using response surface methodology. variables such as substrate concentration, ph, fermentation time and na2hpo4 concentration were found to influence ethanol production significantly. in a batch fermentation, optimization of key process variables resulted in maximum ethanol concentration of 17.44 g/l which was 88% of the theoretical with specific productivity of 0.36 g/l/h. | 2013 | 23710425 |
| wickerhamiella slavikovae sp. nov. and wickerhamiella goesii sp. nov., two yeast species isolated from natural substrates. | two novel yeast species were isolated during three independent studies of yeasts associated with natural substrates in brazil and taiwan. analysis of the sequences of the d1/d2 domains of the large subunit rrna gene showed that these novel species belong to the wickerhamiella clade. the first was isolated from freshwater and a leaf of sugar cane (saccharum officinarum) in brazil and from leaves of wedelia biflora in taiwan. described here as wickerhamiella slavikovae sp. nov., it differs by 56 n ... | 2013 | 23710055 |
| rhamnolipid biosurfactant against fusarium sacchari--the causal organism of pokkah boeng disease of sugarcane. | pokkah boeng disease on sugarcane caused by the fungus fusarium sacchari results considerable damage to the crop leading to top rot, the most serious and advanced stage of pokkah boeng, where the growing point is killed and the entire top of the plant dies. in the present study, the effect of rhamnolipid biosurfactant as an antifungal agent against f. sacchari to control pokkah boeng disease was investigated. on the basis of surface tension reduction, 12 bacterial isolates were selected as poten ... | 2014 | 23687052 |
| ultrasonic pretreatment and acid hydrolysis of sugarcane bagasse for succinic acid production using actinobacillus succinogenes. | immense interest has been devoted to the production of bulk chemicals from lignocellulose biomass. diluted sulfuric acid treatment is currently one of the main pretreatment methods. however, the low total sugar concentration obtained via such pretreatment limits industrial fermentation systems that use lignocellulosic hydrolysate. sugarcane bagasse hemicellulose hydrolysate is used as the carbon and nitrogen sources to achieve a green and economical production of succinic acid in this study. sug ... | 2013 | 23649828 |
| engineered enterobacter aerogenes for efficient utilization of sugarcane molasses in 2,3-butanediol production. | sugarcane molasses is considered to be a good carbon source for biorefinery due to its high sugar content and low price. sucrose occupies more than half of the sugar in the molasses. enterobacter aerogenes is a good host strain for 2,3-butanediol production, but its utilization of sucrose is not very efficient. to improve sucrose utilization in e. aerogenes, a sucrose regulator (scrr) was disrupted from the genomic dna. the deletion mutation increased the sucrose consumption rate significantly w ... | 2013 | 23644066 |
| effects of food processing and fibre content on the digestibility, energy intake and biochemical parameters of blue-and-gold macaws (ara ararauna l. - aves, psittacidae). | considering the increased incidence of obesity and metabolic diseases in caged psittacines, the effect of fibre and food processing was evaluated in the blue-and-gold macaw. four food formulations (0%, 7%, 14% and 21% of sugarcane fibre) processed by pelleting or extrusion were studied, resulting in eight diets. to study digestibility, 48 macaws housed in pairs in cages was used in a block design. subsequently, diets containing 0% or 21% sugarcane fibre, pelleted or extrude was fed for 4 months ... | 2014 | 23627662 |
| identification and characterization of an iron abc transporter operon in gluconacetobacter diazotrophicus pal 5. | gluconacetobacter diazotrophicus is a nitrogen-fixing bacterium and endophyte of sugarcane. we have cloned and sequenced the genes coding for the components of the iron abc-type acquisition system of g. diazotrophicus. sequence analysis revealed three orfs, (feua, feub, and feuc) organized as an operon and encoding polypeptides of 346 (38 kda), 342 (34.2 kda), and 240 (26 kda) amino acids, respectively. the deduced translation products of the feu operon showed similarity with a periplasmic solut ... | 2013 | 23624722 |
| conformational and functional studies of a cytosolic 90 kda heat shock protein hsp90 from sugarcane. | hsp90s are involved in several cellular processes, such as signaling, proteostasis, epigenetics, differentiation and stress defense. although hsp90s from different organisms are highly similar, they usually have small variations in conformation and function. thus, the characterization of different hsp90s is important to gain insight into the structure-function relationship that makes these chaperones key regulators in protein homeostasis. this work describes the characterization of a cytosolic h ... | 2013 | 23619240 |
| microbial degradation of fipronil by bacillus thuringiensis. | fipronil, a phenyl pyrazole insecticide has been found to be effective for the control of various insect pests. due to its higher persistence in soil bioremediation is a promising approach to degrade the pesticide from soil. isolation and identification of soil microbes was conducted for bioremediation of fipronil contaminated soils. soil samples collected from different sugarcane growing fields in gurdaspur district with extensive use of pesticide history served as a source of pesticide degradi ... | 2013 | 23618775 |
| agriculture related corneal injuries. | agricultural workers are predisposed to corneal injuries, which, if neglected, can lead to corneal blindness. | 2015 | 23584646 |
| impact of cefotaxime on somatic embryogenesis and shoot regeneration in sugarcane. | a cephalosporin antibiotic, cefotaxime (omnatax™) promoted somatic embryogenesis and subsequent shoot regeneration in vitro from spindle in sugarcane irrespective of the genotypes as (coj 83, coj 88 and coj 64) culturered on ms medium with 2,4-d (2.5 mgl(-1)) and kinetin (0.5 mgl(-1)). seven different concentrations of cefotaxime (100, 200, 300, 400, 500, 600 and 700 mgl(-1)) were tested to find the optimal concentration of cefotaxime for somatic embryogenesis from callus cultures. among the thr ... | 2009 | 23572935 |
| production of tea vinegar by batch and semicontinuous fermentation. | the fermented tea vinegar combines the beneficial properties of tea and vinegar. the complete fermentation takes 4 to 5 weeks in a batch culture and thus can be shortened by semi continuous/ continuous fermentation using immobilized bacterial cells. in the present study, alcoholic fermentation of 1.0 and 1.5% tea infusions using saccharomyces cerevisae g was carried out that resulted in 84.3 and 84.8% fermentation efficiency (fe) respectively. the batch vinegar fermentation of these wines with a ... | 2010 | 23572817 |
| detoxification of lignocellulosic hydrolysates using sodium borohydride. | addition of sodium borohydride to a lignocellulose hydrolysate of norway spruce affected the fermentability when cellulosic ethanol was produced using saccharomyces cerevisiae. treatment of the hydrolysate with borohydride improved the ethanol yield on consumed sugar from 0.09 to 0.31 g/g, the balanced ethanol yield from 0.02 to 0.30 g/g, and the ethanol productivity from 0.05 to 0.57 g/(l×h). treatment of a sugarcane bagasse hydrolysate gave similar results, and the experiments indicate that so ... | 2013 | 23567704 |
| global analysis of the sugarcane microtranscriptome reveals a unique composition of small rnas associated with axillary bud outgrowth. | axillary bud outgrowth determines shoot architecture and is under the control of endogenous hormones and a fine-tuned gene-expression network, which probably includes small rnas (srnas). although it is well known that srnas act broadly in plant development, our understanding about their roles in vegetative bud outgrowth remains limited. moreover, the expression profiles of micrornas (mirnas) and their targets within axillary buds are largely unknown. here, we employed srna next-generation sequen ... | 2013 | 23564956 |
| mapping the lignin distribution in pretreated sugarcane bagasse by confocal and fluorescence lifetime imaging microscopy. | delignification pretreatments of biomass and methods to assess their efficacy are crucial for biomass-to-biofuels research and technology. here, we applied confocal and fluorescence lifetime imaging microscopy (flim) using one- and two-photon excitation to map the lignin distribution within bagasse fibers pretreated with acid and alkali. the evaluated spectra and decay times are correlated with previously calculated lignin fractions. we have also investigated the influence of the pretreatment on ... | 2013 | 23548159 |
| high-throughput sequencing of small rna transcriptome reveals salt stress regulated micrornas in sugarcane. | salt stress is a primary cause of crop losses worldwide, and it has been the subject of intense investigation to unravel the complex mechanisms responsible for salinity tolerance. microrna is implicated in many developmental processes and in responses to various abiotic stresses, playing pivotal roles in plant adaptation. deep sequencing technology was chosen to determine the small rna transcriptome of saccharum sp cultivars grown on saline conditions. we constructed four small rnas libraries pr ... | 2013 | 23544066 |
| rnai-mediated abrogation of trehalase expression does not affect trehalase activity in sugarcane. | to engineer trehalose metabolism in sugarcane (saccharum spp. hybrids) two transgenes were introduced to the genome: trehalose-6-phosphate synthase- phosphatase (tpsp), to increase trehalose biosynthesis and an rnai transgene specific for trehalase, to abrogate trehalose catabolism. in rnai-expressing lines trehalase expression was abrogated in many plants however no decrease in trehalase activity was observed. in tpsp lines trehalase activity was significantly higher. no events of co-integratio ... | 2012 | 23539210 |
| in situ microscopy: a perspective for industrial bioethanol production monitoring. | this work reviews the state-of-the-art in image-based in situ methods with regard to their potential use for fermentation of saccharomyces cerevisiae in sugarcane wine. the integration of real time information from fermentation tanks in the control strategies has high potential to promote better fermentative performance. while several image-based techniques for the measurement of cell concentration have been established, a reliable and consistent viability measurement still remains a challenging ... | 2013 | 23524154 |
| short-term effects of sugarcane waste products from ethanol production plant as soil amendments on sugarcane growth and metal stabilization. | numerous waste products have been widely studied and used as soil amendments and metal immobilizing agents. waste utilization from ethanol production processes as soil amendments is one of the most promising and sustainable options to help utilize materials effectively, reduce waste disposal, and add value to byproducts. as a consequence, this present work carried out a four-month pot experiment of sugarcane (saccharum officinarum l.) cultivation in cd and zn contaminated soil to determine the e ... | 2013 | 23511210 |
| 5' regulatory region of ubiquitin 2 gene from porteresia coarctata makes efficient promoters for transgene expression in monocots and dicots. | porteresia ubiquitin 5' regulatory region drives transgene expression in monocots and dicots. ubiquitin promoters are promising candidates for constitutive transgene expression in plants. in this study, we isolated and characterized a novel 5' regulatory sequence of a ubiquitin gene from porteresia coarctata, a stress-tolerant wild grass species. through functional analysis in heterologous plant systems, we have demonstrated that full length (port ubi2.3) or truncated sequence (pd2) of the isola ... | 2013 | 23508257 |
| [assessment of health-related quality of life of sugarcane cutters in the pre-harvest and harvest periods]. | to assess health-related quality of life in sugarcane cutters. | 2012 | 23503542 |
| biochemical biomarkers in scinax fuscovarius tadpoles exposed to a commercial formulation of the pesticide fipronil. | one of the main pesticides used in the cultivation of sugarcane in são paulo state, brazil, is regent(®)800wg, the main active compound of which is fipronil. fipronil is a potent insecticide that eliminates pests, including insects resistant to pyrethroids, organophosphates (op) and carbamates (ca). there is little known on the toxic effects of fipronil on non-target organisms, such as tadpoles of frogs. it is possible that this compound carries a high toxicity for these organisms, since the pes ... | 2013 | 23489839 |
| description of a unique, complex feeding socket caused by the putative primitive root-knot nematode, meloidogyne kikuyensis. | meloidogyne kikuyensis produces unique galls that form on one side of the root resembling nitrogen-fixing nodules that are produced on legumes in response to infection by rhizobium and related bacteria. the gall caused by this root-knot nematode is made up of a complex feeding socket composed of several giant cells that are ramified with xylem vessels extending perpendicular from the vascular cylinder. the anterior portion of the second-stage juvenile, which develops into an adult, plugs into th ... | 2012 | 23482409 |
| short-term effects of silage volatile compounds on feed intake and digestion in beef cattle. | two experiments were conducted to evaluate whether fermentation end products in silage affect intake and digestion in beef cattle. six rumen-cannulated nellore steers were randomly assigned to a replicated 3 × 3 latin square design with 14-d periods. each period consisted of 9 d for adaptation and 5 d for sample collection. steers were housed in a tie-stall barn and individually fed once daily at 0800 h. the dietary treatments in exp. 1 were as follows: 60% corn silage plus 40% concentrate (con) ... | 2013 | 23478815 |
| study of kinetic parameters in a mechanistic model for enzymatic hydrolysis of sugarcane bagasse subjected to different pretreatments. | the goal of this work is to evaluate the influence of different pretreatments in the kinetics of enzymatic hydrolysis of sugarcane bagasse and to propose a reliable methodology to easily perform sensitivity analysis and updating kinetic parameters whenever necessary. a kinetic model was modified to represent the experimental data of the batch enzymatic hydrolysis of sugarcane bagasse pretreated with alkaline hydrogen peroxide. the simultaneous estimation of kinetic parameters of the mathematical ... | 2013 | 23474967 |
| tailor-made fructan synthesis in plants: a review. | fructan, a fructose polymer, is produced by many bacteria and plants. fructan is used as carbohydrate reserve, and in bacteria also as protective outside layer. chicory is a commercial fructan producing crop. the disadvantage of this crop is its fructan breakdown before harvest. studies using genetically modification showed that fructan biosynthesis is difficult to steer in chicory. alternatives for production of tailor-made fructan, fructan with a desired polymer length and linkage type, are or ... | 2012 | 23465900 |
| occurrence and larval movement of diatraea saccharalis (lepidoptera: crambidae) in seed mixes of non-bt and bt pyramid corn. | larval movement of target pest populations among bt and non-bt plants is a major concern in the use of a seed mixture refuge strategy for bt resistance management. in this study, occurrence and larval movement of the sugarcane borer, diatraea saccharalis (f.), were evaluated in four planting patterns of non-bt and bt plants containing genuity® smartstax(tm) traits in 2009-2011. the four planting patterns were: (1) a pure stand of 27 bt plants; (2) one non-bt plant in the center, surrounded by 26 ... | 2013 | 23456950 |
| the influence of nitrate on the physiology of the yeast dekkera bruxellensis grown under oxygen limitation. | a previous study showed that the use of nitrate by dekkera bruxellensis might be an advantageous trait when ammonium is limited in sugarcane substrate for ethanol fermentation. the aim of the present work was to evaluate the influence of nitrate on the yeast physiology during cell growth in different carbon sources under oxygen limitation. if nitrate was the sole source of nitrogen, d. bruxellensis cells presented slower growth, diminished sugar consumption and growth-associated ethanol producti ... | 2013 | 23440690 |
| factors associated with the suppressiveness of sugarcane soils to plant-parasitic nematodes. | observations in three australian sugarcane fields suggested that the soil just under the trash blanket (the covering of crop residue that remains on the soil surface after crops are harvested) was suppressive to plant-parasitic nematodes. roots were concentrated in this upper layer of soil but plant-parasitic nematode populations were relatively low and roots showed few signs of nematode damage. root biomass was much lower 15 cm further down the soil profile, where root health was poor and popul ... | 2011 | 23431051 |
| genetic diversity of sugarcane bacilliform virus isolates infecting saccharum spp. in india. | sugarcane bacilliform virus (scbv), which causes leaf freckle in sugarcane, is a member of the genus badnavirus. studies were conducted to characterize scbv in saccharum officinarum germplasm and cultivated varieties in india by sequencing the complete genomes of five isolates. genome lengths ranged from 7,553 to 7,884 nucleotides. duplications in orf3 and insertions in the rnase h-domain in some of the isolates were found to contribute to the large size of their genomes. the indian scbv isolate ... | 2013 | 23430710 |
| enhanced cellulase production by trichoderma viride in a rotating fibrous bed bioreactor. | filamentous fungi are widely used to produce cellulase, but how the fermentation conditions affect their production is not well known. in this study, cellulase production by trichoderma viride in submerged fermentations with free cells in a stirred-tank reactor (str) and immobilized cells in a rotating fibrous-bed bioreactor (rfbb) were investigated. compared to free-cell fermentation, immobilized-cell fermentation gave 35.5% higher fpase activity and 69.7% higher saccharification yield of sugar ... | 2013 | 23428816 |
| silencing of molt-regulating transcription factor gene, cihr3, affects growth and development of sugarcane stem borer, chilo infuscatellus. | rna interference (rnai) is a technology for conducting functional genomic studies and a potential tool for crop protection against insect pests. development of reliable methods for production and delivery of double-stranded rna (dsrna) is the major challenge for efficient pest control. in this study, chilo infuscatellus snellen (crambidae: lepidoptera) was fed with cihr3 dsrna expressed in bacteria or synthesized in vitro. the dsrna ingested by c. infuscatellus successfully triggered silencing o ... | 0 | 23427912 |
| stimulation of extracellular invertase production from spent yeast when sugarcane pressmud used as substrate through solid state fermentation. | efforts were made to utilize the waste/by-product of two agro-process industries namely pressmud from sugar processing industries and spent yeast from distilleries manufacturing ethanol from cane molasses, for the production of microbial invertase. our experimentation indicated that these two sources could be ideally utilized for the production of invertase through solid substrate fermentation (ssf). ssf with spent yeast had given highest specific activity of 430 u/mg in 72 h of fermentation. in ... | 2012 | 23420549 |
| expression of soflac, a new laccase in sugarcane, restores lignin content but not s:g ratio of arabidopsis lac17 mutant. | lignin is a complex phenolic heteropolymer deposited in the secondarily thickened walls of specialized plant cells to provide strength for plants to stand upright and hydrophobicity to conducting cells for long-distance water transport. although essential for plant growth and development, lignin is the major plant cell-wall component responsible for biomass recalcitrance to industrial processing. peroxidases and laccases are generally thought to be responsible for lignin polymerization, but, giv ... | 2013 | 23418623 |
| repeated batch cell-immobilized system for the biotechnological production of xylitol as a renewable green sweetener. | the present paper studies the biotechnological production of xylitol using sugarcane bagasse hydrolysate in a repeated batch fermentation system with immobilized cells of candida guilliermondii fti20037. immobilized cell system is considered as an attractive alternative to reuse the well-grown and adapted yeast cells in a new fresh fermentation media, without the need of the inoculum stage. in this work, seven repeated batches were performed in a fluidized bed bioreactor using immobilized cells ... | 2013 | 23397324 |
| understanding the cellulolytic system of trichoderma harzianum p49p11 and enhancing saccharification of pretreated sugarcane bagasse by supplementation with pectinase and α-l-arabinofuranosidase. | supplementation of cellulase cocktails with accessory enzymes can contribute to a higher hydrolytic capacity in releasing fermentable sugars from plant biomass. this study investigated which enzymes were complementary to the enzyme set of trichoderma harzianum in the degradation of sugarcane bagasse. specific activities of t. harzianum extract on different substrates were compared with the extracts of penicillium echinulatum and trichoderma reesei, and two commercial cellulase preparations. comp ... | 2013 | 23391738 |
| comparative metagenomic analysis of microcosm structures and lignocellulolytic enzyme systems of symbiotic biomass-degrading consortia. | decomposition of lignocelluloses by cooperative microbial actions is an essential process of carbon cycling in nature and provides a basis for biomass conversion to fuels and chemicals in biorefineries. in this study, structurally stable symbiotic aero-tolerant lignocellulose-degrading microbial consortia were obtained from biodiversified microflora present in industrial sugarcane bagasse pile (bgc-1), cow rumen fluid (crc-1), and pulp mill activated sludge (asc-1) by successive subcultivation o ... | 2013 | 23381385 |
| exploiting est databases for the development and characterisation of 3425 gene-tagged cisp markers in biofuel crop sugarcane and their transferability in cereals and orphan tropical grasses. | sugarcane is an important cash crop, providing 70% of the global raw sugar as well as raw material for biofuel production. genetic analysis is hindered in sugarcane because of its large and complex polyploid genome and lack of sufficiently informative gene-tagged markers. modern genomics has produced large amount of ests, which can be exploited to develop molecular markers based on comparative analysis with est datasets of related crops and whole rice genome sequence, and accentuate their cross- ... | 0 | 23379891 |
| a 24.7-kda copper-containing oxidase, secreted by thermobifida fusca, significantly increasing the xylanase/cellulase-catalyzed hydrolysis of sugarcane bagasse. | thermobifida fusca is a moderately thermophilic soil bacterium belonging to actinobacteria. it has been known for its capability to degrade plant cell wall polymers except lignin and pectin. to know whether it can produce enzymes to facilitate lignin degradation, the extracellular proteins bound to sugarcane bagasse were harvested and identified by liquid chromatography tandem mass spectrometry. among the identified proteins, a putative copper-containing polyphenol oxidase of 241 amino acids, en ... | 2013 | 23377789 |
| new claims in folk veterinary medicines from uttar pradesh, india. | in remote areas, folk medicines play a significant role in livestock health care management. the aims of this study were to document folk veterinary medicines of the uttar pradesh region and identify promising species for pharmacological studies. | 2013 | 23376281 |
| optimization of β-glucosidase, β-xylosidase and xylanase production by colletotrichum graminicola under solid-state fermentation and application in raw sugarcane trash saccharification. | efficient, low-cost enzymatic hydrolysis of lignocellulosic residues is essential for cost-effective production of bioethanol. the production of β-glucosidase, β-xylosidase and xylanase by colletotrichum graminicola was optimized using response surface methodology (rsm). maximal production occurred in wheat bran. sugarcane trash, peanut hulls and corncob enhanced β-glucosidase, β-xylosidase and xylanase production, respectively. maximal levels after optimization reached 159.3 ± 12.7 u g-1, 128.1 ... | 2013 | 23364611 |
| transcriptional profiling of brazilian saccharomyces cerevisiae strains selected for semi-continuous fermentation of sugarcane must. | brazil played a pioneering role in the global establishment of the sugarcane bioethanol industry. the bioethanol fermentation process currently used in brazil is unique due to the acid wash and recycling of yeast cells. two, industrially adopted, wild yeast strains, cat-1 and pe-2, have become the most widely used in brazil. how these strains respond to the unique fermentation process is poorly understood. the improved performance of cat-1 and pe-2 is hypothesised to be related to enhanced stres ... | 2013 | 23360418 |
| in vivo interactions of entomopathogenic fungi, beauveria spp. and metarhizium anisopliae with selected opportunistic soil fungi of sugarcane ecosystem. | in the present study, the interactions of entomopathogenic fungi viz., beauveria bassiana, beauveria brongniartii and metarhizium anisopliae among themselves and three other opportunistic soil fungi from the sugarcane ecosystem namely, fusarium saachari, aspergillus sp. and penecillium sp. were assayed in vivo against galleria mellonella larvae. the tested fungi were co-applied on iv instar g. mellonella @ 1 x 10(7) ml(-1), in combinations of two, at the interval of 24 hrs either preceding or su ... | 2012 | 23359998 |
| enhancing polyhydroxybutyrate production from high cell density fed-batch fermentation of bacillus megaterium ba-019. | this study demonstrated the improved polyhydroxybutyrate (phb) production via high cell density cultivation of bacillus megaterium ba-019 with balanced initial total sugar concentration and carbon to nitrogen (c/n) weight ratio. in the 10 l stirred fermentor operated at 30 °c, ph 7.0, 600 rpm, and 1.0 vvm air, with the initial total sugar concentration of 60 g/l and urea at the c/n weight ratio of 10:1, 32.48 g/l cell biomass with the corresponding phb weight content of 26.94 % and volumetric pr ... | 2013 | 23340799 |
| lime pretreatment and fermentation of enzymatically hydrolyzed sugarcane bagasse. | sugarcane bagasse was subjected to lime (calcium hydroxide) pretreatment and enzymatic hydrolysis for second-generation ethanol production. a central composite factorial design was performed to determine the best combination of pretreatment time, temperature, and lime loading, as well as to evaluate the influence of enzymatic loadings on hydrolysis conversion. the influence of increasing solids loading in the pretreatment and enzymatic hydrolysis stages was also determined. the hydrolysate was f ... | 2013 | 23334836 |
| recovery of bovine lysozyme from transgenic sugarcane stalks: extraction, membrane filtration, and purification. | increased industrial use of sugarcane (saccharum spp. hybrid) for food and bioenergy has led to considerable improvements in its genetic transformation, which allowed the development of not only pest- and herbicide-resistant lines but also lines expressing high-value bioproducts and biopolymers. however, the economic benefits of using inexpensive transgenic plant systems for the production of industrial proteins could be offset by high downstream processing costs. in this work, transgenic sugarc ... | 2013 | 23329238 |
| ultra-structural mapping of sugarcane bagasse after oxalic acid fiber expansion (oafex) and ethanol production by candida shehatae and saccharomyces cerevisiae. | diminishing supplies of fossil fuels and oil spills are rousing to explore the alternative sources of energy that can be produced from non-food/feed-based substrates. due to its abundance, sugarcane bagasse (sb) could be a model substrate for the second-generation biofuel cellulosic ethanol. however, the efficient bioconversion of sb remains a challenge for the commercial production of cellulosic ethanol. we hypothesized that oxalic-acid-mediated thermochemical pretreatment (oafex) would overcom ... | 2013 | 23324164 |
| chrysoporthe cubensis: a new source of cellulases and hemicellulases to application in biomass saccharification processes. | the plant pathogenic fungus chrysoporthe cubensis was cultivated under solid state employing different substrates and the highest endoglucanase (33.84ug(-1)), fpase (2.52ug(-1)), β-glucosidase (21.55ug(-1)) and xylanase (362.38ug(-1)) activities were obtained using wheat bran as carbon source. cellulases and xylanase produced by c. cubensis showed maximal hydrolysis rate at ph 4.0 and in a temperature range of 50-60°c. all enzymatic activities were highly stable at 40 and 50°c through 48h of pre ... | 2012 | 23313674 |
| isolation and characterisation of a ferrirhodin synthetase gene from the sugarcane pathogen fusarium sacchari. | fsn1, a gene isolated from the sugar-cane pathogen fusarium sacchari, encodes a 4707-residue nonribosomal peptide synthetase consisting of three complete adenylation, thiolation and condensation modules followed by two additional thiolation and condensation domain repeats. this structure is similar to that of ferricrocin synthetase, which makes a siderophore that is involved in intracellular iron storage in other filamentous fungi. heterologous expression of fsn1 in aspergillus oryzae resulted i ... | 2013 | 23307607 |
| production and partial characterization of cellulases and xylanases from trichoderma atroviride 676 using lignocellulosic residual biomass. | trichoderma atroviride 676 was studied to evaluate its efficiency in the production of some lignocellulolytic enzymes, using lignocellulosic residual biomass. best results were obtained when 3.0 % (w/v) untreated sugarcane bagasse was used (61.3 u ml(-1) for xylanase, 1.9 u ml(-1) for endoglucanase, 0.25 u ml(-1) for fpase, and 0.17 u ml(-1) for β-glucosidase) after 3-4 days fermentation. the maximal enzymatic activity for endoglucanase, fpase, and xylanase were observed at 50-60 °c and ph 4.0-5 ... | 2013 | 23306885 |
| enterobacter sacchari sp. nov., a nitrogen-fixing bacterium associated with sugar cane (saccharum officinarum l.). | five nitrogen-fixing bacterial strains (sp1(t), nn143, nn144, nn208 and hx148) were isolated from stem, root or rhizosphere soil of sugar cane (saccharum officinarum l.) plants. cells were gram-negative, motile, rods with peritrichous flagella. dna g+c content was 55.0 ± 0.5 mol%. sequence determinations and phylogenetic analysis of 16s rrna gene and rpob indicated that the strains were affiliated with the genus enterobacter and most closely related to e. radicincitans dsm 16656(t) and e. oryzae ... | 2013 | 23291881 |
| using exploratory factor analysis of ffq data to identify dietary patterns among yup'ik people. | an ffq developed by the center for alaska native health research for studies in yup'ik people includes market foods and subsistence foods such as moose, seal, waterfowl and salmon that may be related to disease risk. because the ffq contains >100 food items, we sought to characterize dietary patterns more simply for use in ongoing pharmacogenomics studies. | 2014 | 23290469 |
| solvent extraction of antioxidants from steam exploded sugarcane bagasse and enzymatic convertibility of the solid fraction. | solvent extraction of steam exploded lignocellulosic biomass may be a potential way to obtain antioxidative extracts and to enhance the enzymatic convertibility of the solid residue. boiling solvent extraction (bse) showed higher solid and phenolic yields than room temperature extraction. solubilities of phenolics and sugars were higher in anhydrous ethanol (ae) and deionized water (dw) than in ethyl acetate under each individual extraction condition. the antioxidant activities of the ae and dw ... | 2012 | 23280180 |
| what do we know about the yeast strains from the brazilian fuel ethanol industry? | the production of fuel ethanol from sugarcane-based raw materials in brazil is a successful example of a large-scale bioprocess that delivers an advanced biofuel at competitive prices and low environmental impact. two to three fed-batch fermentations per day, with acid treatment of the yeast cream between consecutive cycles, during 6-8 months of uninterrupted production in a nonaseptic environment are some of the features that make the brazilian process quite peculiar. along the past decades, so ... | 2013 | 23271669 |
| susceptibility of cry1ab maize-resistant and -susceptible strains of sugarcane borer (lepidoptera: crambidae) to four individual cry proteins. | sugarcane borer, diatraea saccharalis (f.), is a major target of bt maize in south america and many areas of the us mid-south region. six laboratory strains of d. saccharalis were established from six single-pair f(2) families possessing major resistance alleles to cry1ab maize hybrids. susceptibility of the six strains was evaluated on diet treated with each of four purified trypsin-activated bt proteins, cry1ab, cry1aa, cry1ac and cry1f. bt susceptibility of the six strains was compared with t ... | 2013 | 23270874 |
| use of different extracts of coffee pulp for the production of bioethanol. | coffee is one of the most important agricultural products in brazil. more than 50 % of the coffee fruit is not used for the production of commercial green coffee and is therefore discarded, usually ending up in the environment. the goal of this work was to select an efficient process for obtaining coffee pulp extract and to evaluate the use of this extract in bioethanol production. the effects of heat treatment and trituration on the yield and composition of the extract were investigated by meas ... | 2013 | 23269634 |
| production of methanol from biomass waste via pyrolysis. | the production of methanol from agricultural, forestry, livestock, poultry, and fishery waste via pyrolysis was investigated. pyrolysis was conducted in a tube furnace at 450-500 °c. sugarcane bagasse showed the methanol production (5.93 wt.%), followed by roots and sawdust with 4.36 and 4.22 wt.%, respectively. animal waste offered the lowest content of methanol, as only 0.46, 0.80, and 0.61 wt.% were obtained from fishery, goat, and cow waste, respectively. it was also observed that the percen ... | 2013 | 23266847 |
| experimental mixture design as a tool to enhance glycosyl hydrolases production by a new trichoderma harzianum p49p11 strain cultivated under controlled bioreactor submerged fermentation. | this work investigates the glycosyl hydrolase (gh) profile of a new trichoderma harzianum strain cultivated under controlled bioreactor submerged fermentation. the influence of different medium components (delignified steam-exploded sugarcane bagasse, sucrose, and soybean flour) on gh biosynthesis was assessed using experimental mixture design (emd). additionally, the effect of increased component concentrations in culture media selected from the emd was studied. it was found that that a mixed c ... | 2013 | 23265822 |
| sugarcane bagasse treated with hydrous ferric oxide as a potential adsorbent for the removal of as(v) from aqueous solutions. | the mechanism of as(v) removal from aqueous solutions by means of hydrated ferric oxide (hfo)-treated sugarcane bagasse (scb-hfo) (saccharum officinarum l.) was investigated. effects of different parameters, such as ph value, initial arsenic concentration, adsorbent dosage, contact time and ionic strength, on the as(v) adsorption were studied. the adsorption capacity of scb-hfo for as(v) was found to be 22.1 mg/g under optimum conditions of ph 4, contact time 3h and temperature 22 °c. initial as ... | 2013 | 23265467 |
| coupled production of single cell oil as biodiesel feedstock, xylitol and xylanase from sugarcane bagasse in a biorefinery concept using fungi from the tropical mangrove wetlands. | this work evaluates sugarcane bagasse (scb) conversion, in a biorefinery approach, to coproduce biodiesel and high value products using two novel mangrove fungi. on acid pre-treatment, sugarcane bagasse hydrolysate (scbh) resulted in a xylitol yield of 0.51 g/g xylose consumed in 72 h by williopsis saturnus. after scb pretreatment, sugarcane bagasse residue (scbr) was utilized using aspergillus terreus for production of xylanase (12.74 u/ml) and cell biomass (9.8 g/l) which was extracted for sin ... | 2013 | 23260270 |
| bioconversion of sugarcane biomass into ethanol: an overview about composition, pretreatment methods, detoxification of hydrolysates, enzymatic saccharification, and ethanol fermentation. | depleted supplies of fossil fuel, regular price hikes of gasoline, and environmental damage have necessitated the search for economic and eco-benign alternative of gasoline. ethanol is produced from food/feed-based substrates (grains, sugars, and molasses), and its application as an energy source does not seem fit for long term due to the increasing fuel, food, feed, and other needs. these concerns have enforced to explore the alternative means of cost competitive and sustainable supply of biofu ... | 2012 | 23251086 |
| x-ray crystallography and nmr studies of domain-swapped canecystatin-1. | the three-dimensional structure of canecystatin-1, a potent inhibitor of cysteine proteases from sugarcane (saccharum officinarum), has been solved in two different crystal forms. in both cases, it is seen to exist as a domain-swapped dimer, the first such observation for a cystatin of plant origin. size exclusion chromatography and multidimensional nmr spectroscopy show the dimer to be the dominant species in solution, despite the presence of a measurable quantity of monomer undergoing slow exc ... | 2013 | 23241243 |
| biomass-to-bio-products application of feruloyl esterase from aspergillus clavatus. | the structural polysaccharides contained in plant cell walls have been pointed to as a promising renewable alternative to petroleum and natural gas. ferulic acid is a ubiquitous component of plant polysaccharides, which is found in either monomeric or dimeric forms and is covalently linked to arabinosyl residues. ferulic acid has several commercial applications in food and pharmaceutical industries. the study herein introduces a novel feruloyl esterase from aspergillus clavatus (acfae). along wi ... | 2013 | 23229566 |
| correlation between agar plate screening and solid-state fermentation for the prediction of cellulase production by trichoderma strains. | the viability of converting biomass into biofuels and chemicals still requires further development towards the reduction of the enzyme production costs. thus, there is a growing demand for the development of efficient procedures for selection of cellulase-producing microorganisms. this work correlates qualitative screening using agar plate assays with quantitative measurements of cellulase production during cultivation under solid-state fermentation (ssf). the initial screening step consisted of ... | 2012 | 23227312 |
| genome sequence of enterobacter sp. strain sp1, an endophytic nitrogen-fixing bacterium isolated from sugarcane. | enterobacter sp. strain sp1 is an endophytic nitrogen-fixing bacterium isolated from a sugarcane stem and can promote plant growth. the draft genome sequence of strain sp1 presented here will promote comparative genomic studies to determine the genetic background of interactions between endophytic enterobacteria and plants. | 2012 | 23209221 |
| yamadazyma siamensis sp. nov., yamadazyma phyllophila sp. nov. and yamadazyma paraphyllophila sp. nov., three novel yeast species isolated from phylloplane in thailand and taiwan. | four strains representing three novel anamorphic yeast species were isolated from the external surface of sugarcane leaves (dmku-rk254(t)), corn leaves (dmku-rk548(t)), bean leaves (k129) in thailand and hengchun pencilwood leaves (trb1-1(t)) in taiwan. on the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, the sequence analysis of the d1/d2 region of the large subunit (lsu) rrna gene, the internal transcribed spacer (its) region, the actin gene (act1) and ... | 2013 | 23208204 |
| increase in ethanol production from sugarcane bagasse based on combined pretreatments and fed-batch enzymatic hydrolysis. | enzymatic hydrolysis of pretreated sugarcane bagasse was performed to investigate the production of ethanol. the sugarcane bagasse was pretreated in a process combining steam explosion and alkaline delignification. the lignin content decreased to 83%. fed-batch enzymatic hydrolyses was initiated with 8% (w/v) solids loading, and 10 fpu/g cellulose. then, 1% solids were fed at 12, 24 or 48 h intervals. after 120 h, the hydrolysates were fermented with saccharomyces cerevisiae ufpeda 1238, and a f ... | 2013 | 23201527 |
| gene silencing in root lesion nematodes (pratylenchus spp.) significantly reduces reproduction in a plant host. | root lesion nematodes (rlns, pratylenchus species) are a group of economically important migratory endoparasitic plant pathogens that attack host roots of major crops such as wheat and sugarcane, and can reduce crop yields by 7-15%. pratylenchus thornei and pratylenchus zeae were treated with double stranded rna (dsrna) to study gene silencing, (rna interference, rnai), as a potential strategy for their control. mixed stages of nematodes of both species ingested dsrna when incubated in a basic s ... | 2013 | 23201220 |
| enzymatic saccharification of sugarcane bagasse by n-methylmorpholine-n-oxide-tolerant cellulase from a newly isolated galactomyces sp. cczu11-1. | based on the enrichment culture strategy, a novel n-methylmorpholine-n-oxide (nmmo)-tolerant cellulase-producing strain galactomyces sp. cczu11-1 was isolated from soil samples. after the optimization of culture condition, the highest fpa (13.4 u/ml) and cmcase (24.5 u/ml) were obtained. in both culture and reaction media containing nmmo 25% (w/v), the cellulase from galactomyces sp. cczu11-1 still had good activity. furthermore, high saccharification rate was obtained in aqueous-nmmo media. mor ... | 2013 | 23186661 |
| cellulase production from a new strain acremonium strictum isolated from the brazilian biome using different substrates. | the objective of the present study was to evaluate the production of cellulolytic enzymes by acremonium strictum isolated from brazilian biome using different substrates. fermentations were initially carried out using commercial substrates, including microcrystalline cellulose (avicel® and servacel®) and carboxymethylcellulose (cmc). this was followed by fermentations performed using lignocellulosic biomass: sugarcane bagasse pretreated at different intensities. the fermentations were carried ou ... | 2013 | 23186655 |
| genomic insights into strategies used by xanthomonas albilineans with its reduced artillery to spread within sugarcane xylem vessels. | xanthomonas albilineans causes leaf scald, a lethal disease of sugarcane. x. albilineans exhibits distinctive pathogenic mechanisms, ecology and taxonomy compared to other species of xanthomonas. for example, this species produces a potent dna gyrase inhibitor called albicidin that is largely responsible for inducing disease symptoms; its habitat is limited to xylem; and the species exhibits large variability. a first manuscript on the complete genome sequence of the highly pathogenic x. albilin ... | 2012 | 23171051 |