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evaluating korean rice genotypes and landraces for octacosanol contents and antioxidant activity.increase in the average life span and interest in correlation between food and human health have led to researches to find and utilise the functional materials in rice. octacosanol in rice grain is well known for its potential benefit. this study was conducted to evaluate the korean rice (oryza sativa l.) genotypes and landraces for octacosanol contents and antioxidant activity. octacosanol contents in 223 cultivars of korean rice ranged from 0.78 to 6.06 mg 100 g-1. thirteen genotypes and landr ...201728278625
harnessing the hidden genetic diversity for improving multiple abiotic stress tolerance in rice (oryza sativa l.).to develop superior rice varieties with improved yield in most rainfed areas of asia/africa, we started an introgression-breeding program for simultaneously improving yield and tolerances of multiple abiotic stresses. using eight bc1 populations derived from a widely adaptable recipient and eight donors plus three rounds of phenotypic selection, we developed 496 introgression lines (ils) with significantly higher yield under drought, salt and/or non-stress conditions in 5 years. six new varietie ...201728278154
transposon-mediated epigenetic regulation contributes to phenotypic diversity and environmental adaptation in rice.transposable elements (tes) have long been regarded as 'selfish dna', and are generally silenced by epigenetic mechanisms. however, work in the past decade has identified positive roles for tes in generating genomic novelty and diversity in plants. in particular, recent studies suggested that te-induced epigenetic alterations and modification of gene expression contribute to phenotypic variation and adaptation to geography or stress. these findings have led many to regard tes, not as junk dna, b ...201728273484
transcript profiling reveals the presence of abiotic stress and developmental stage specific ascorbate oxidase genes in plants.abiotic stress and climate change is the major concern for plant growth and crop yield. abiotic stresses lead to enhanced accumulation of reactive oxygen species (ros) consequently resulting in cellular damage and major losses in crop yield. one of the major scavengers of ros is ascorbate (aa) which acts as first line of defense against external oxidants. an enzyme named ascorbate oxidase (aao) is known to oxidize aa and deleteriously affect the plant system in response to stress. genome-wide an ...201728261251
aluminum enhances growth and sugar concentration, alters macronutrient status and regulates the expression of nac transcription factors in rice.aluminum (al) is a beneficial element for some plant species, especially when used at low concentrations. though some transcription factors are induced by exposure to this element, no data indicate that al regulates the expression of nac genes in rice. in this study we tested the effect of applying 200 μm al on growth, chlorophyll, amino acids, sugars, macronutrient concentration and regulation of nac transcription factors gene expression in 24-day-old plants of four rice (oryza sativa ssp. indi ...201728261224
is early morning flowering an effective trait to minimize heat stress damage during flowering in rice?higher spikelet sterility due to heat stress exposure during flowering in rice is becoming a major threat for sustaining productivity in tropical and sub-tropical regions. therefore, exploiting and incorporating early morning flowering (emf) trait into ongoing breeding pipelines could be an effective strategy to minimize the damage. in this study, we have focused on quantifying the time of day of flowering traits such as first spikelet opening time (fsot) and peak spikelet opening time (psot) in ...201728260830
the important functionality of 14-3-3 isoforms in rice roots revealed by affinity chromatography.plant 14-3-3 proteins belong to a large family of proteins involved in numerous physiological processes, and function by binding to phosphorylated client proteins to regulate their function. however, little is known about their regulatory mechanisms in rice root growth. in this study, four 14-3-3 isoforms (gf14b, gf14c, gf14e, gf14f) exhibiting prominent expression profiles in rice roots, were selected for further investigation. through a pull-down assay using four 14-3-3 isoforms in rice roots, ...201728254371
characterization of a disease susceptibility locus for exploring an efficient way to improve rice resistance against bacterial blight.bacterial blight caused by xanthomonas oryzae pv. oryzae (xoo) is the most harmful bacterial disease of rice worldwide. previously, we characterized major disease resistance (mr) gene xa25, which confers race-specific resistance to xoo strain pxo339. the xa25 is a recessive allele of the sweet13 locus, but sweet13's interaction with pxo339 and how efficiently using this locus for rice breeding still need to be defined. here we show that the sweet13 allele from rice zhenshan 97 is a susceptibilit ...201728251460
physiological performance of two contrasting rice varieties under water stress.two rice varieties pr-115 and super-7 were imposed to water stress and different physiological traits were monitored to evaluate the performance of these varieties under drought. under water stress condition although the relative water content, osmotic potential, chlorophyll content, photosynthesis rate, carbon discrimination and biomass decreased in both the varieties however, the reduction was more pronounced in super-7 variety. oryzanol a trans-ester of ferulic acid functions as antioxidant a ...201728250586
mitochondrial abc transporter atm3 is essential for cytosolic iron-sulfur cluster assembly.the mitochondrial atp-binding cassette transporter atm3 has been studied in arabidopsis. its function, however, is poorly understood in other model plant species. this study reports that the atm3 is required for cytosolic iron-sulfur cluster assembly and is essential for meristem maintenance in rice (oryza sativa). the loss of function of osatm3 is lethal in rice at the four-leaf stage. in the osatm3 t-dna insertion mutant, the fourth leaf fails to develop and the lateral roots are short. cytoso ...201728250070
hybrid weakness in a rice interspecific hybrid is nitrogen-dependent, and accompanied by changes in gene expression at both total transcript level and parental allele partitioning.hybrid weakness, a phenomenon opposite to heterosis, refers to inferior growth and development in a hybrid relative to its pure-line parents. little attention has been paid to the phenomenological or mechanistic aspect of hybrid weakness, probably due to its rare occurrence.201728248994
tsv, a putative plastidic oxidoreductase, protects rice chloroplasts from cold stress during development by interacting with plastidic thioredoxin z.rice is vulnerable to cold stress. seedlings are very sensitive to cold stress and this harms global rice production. the effects of cold on chloroplast development are well known, but little is known about the underlying molecular mechanisms. here, we isolated a temperature-sensitive virescent (tsv) mutant that is extremely sensitive to cold stress. it displayed defective chloroplasts, decreased chlorophyll and zero survivorship under cold stress. we isolated and identified tsv by map-based clo ...201728248438
gene expression and plant hormone levels in two contrasting rice genotypes responding to brown planthopper infestation.the brown planthopper (bph; nilaparvata lugens stål) is a destructive piercing-sucking insect pest of rice. the plant hormones salicylic acid (sa) and jasmonic acid (ja) play important roles in plant-pest interactions. many isolated rice genes that modulate bph resistance are involved in the metabolism or signaling pathways of sa, ja and ethylene. 'rathu heenati' (rh) is a rice cultivar with a high-level, broad-spectrum resistance to all bph biotypes. here, rh was used as the research material, ...201728245796
simultaneous profiling of lysoglycerophospholipids in rice (oryza sativa l.) using direct infusion-tandem mass spectrometry with multiple reaction monitoring.white rice is the final product after the hull and bran layers have been removed during the milling process. although lysoglycerophospholipids (lysogpls) only occupy a small proportion in white rice, they are essential for evaluating rice authenticity and quality. in this study, we developed a high-throughput and targeted lipidomics approach that involved direct infusion-tandem mass spectrometry with multiple reaction monitoring to simultaneously profile lysogpls in white rice. the method is cap ...201728245645
molecular structure and physicochemical properties of starches from rice with different amylose contents resulting from modification of osgbssi activity.osgbssi, encoded by the waxy (wx) gene, is the key enzyme in the synthesis of amylose chains. transgenic rice lines with various gbssi activities were previously developed via site-directed mutagenesis of the wx gene in the glutinous cultivar guanglingxiangnuo (glxn). in this study, grain morphology, molecular structure, and physicochemical properties were investigated in four transgenic lines with modified osgbssi activity and differences in amylose content. a milky opaque appearance was observ ...201728241110
estimation of loci involved in non-shattering of seeds in early rice domestication.rice (oryza sativa l.) is widely cultivated around the world and is known to be domesticated from its wild form, o. rufipogon. a loss of seed shattering is one of the most obvious phenotypic changes selected for during rice domestication. previously, three seed-shattering loci, qsh1, sh4, and qsh3 were reported to be involved in non-shattering of seeds of japonica-type cultivated rice, o. sativa cv. nipponbare. in this study, we focused on non-shattering characteristics of o. sativa indica cv. i ...201728238052
spatial imaging and speciation of cu in rice (oryza sativa l.) roots using synchrotron-based x-ray microfluorescence and x-ray absorption spectroscopy.knowledge of elemental localization and speciation in rice (oryza sativa l.) roots is crucial for elucidating the mechanisms of cu accumulation so as to facilitate the development of strategies to inhibit cu accumulation in rice grain grown in contaminated soils. using synchrotron-based x-ray microfluorescence and x-ray absorption spectroscopy, we investigated the distribution patterns and speciation of cu in rice roots treated with 50 μm cu for 7 days. a clear preferential localization of cu in ...201728235745
altered expression profiles of microrna families during de-etiolation of maize and rice leaves.micrornas (mirnas) are highly conserved small non-coding rnas that play important regulatory roles in plants. although many mirna families are sequentially and functionally conserved across plant kingdoms (dezulian et al. in genome biol 13, 2005), they still differ in many aspects such as family size, average length, genomic loci etc. (unver et al. in int j plant genomics, 2009).201728235420
pyricularia graminis-tritici, a new pyricularia species causing wheat blast.pyricularia oryzae is a species complex that causes blast disease on more than 50 species of poaceous plants. pyricularia oryzae has a worldwide distribution as a rice pathogen and in the last 30 years emerged as an important wheat pathogen in southern brazil. we conducted phylogenetic analyses using 10 housekeeping loci for 128 isolates of p. oryzae sampled from sympatric populations of wheat, rice, and grasses growing in or near wheat fields. phylogenetic analyses grouped the isolates into thr ...201628232765
mechanisms of fe biofortification and mitigation of cd accumulation in rice (oryza sativa l.) grown hydroponically with fe chelate fertilization.cadmium contaminated rice from china has become a global food safety issue. some research has suggested that chelate addition to substrates can affect metal speciation and plant metal content. we investigated the mitigation of cd accumulation in hydroponically-grown rice supplied with edtana2fe(ii) or eddhafe(iii). a japonica rice variety (nipponbare) was grown in modified kimura b solution containing three concentrations (0, 10, 100 μμ) of the iron chelates edtana2fe(ii) or eddhafe(iii) and 1 μ ...201728232138
accurate measurement of transgene copy number in crop plants using droplet digital pcr.genetic transformation is a powerful means for the improvement of crop plants, but requires labor- and resource-intensive methods. an efficient method for identifying single-copy transgene insertion events from a population of independent transgenic lines is desirable. currently, transgene copy number is estimated by either southern blot hybridization analyses or quantitative polymerase chain reaction (qpcr) experiments. southern hybridization is a convincing and reliable method, but it also is ...201728231382
volatile composition of smoked and non-smoked iranian rice.in this work, the volatile profiles of smoked and non-smoked iranian rice were identified, and their relative abundance was calculated and compared. headspace solid-phase microextraction together with gas chromatography-mass spectrometry (spme-gc-ms) were used to extract and identify the volatile compounds. the main groups of volatiles in iranian rice were aldehydes, ketones, phenol derivatives, furans, linear hydrocarbons, esters and terpenes. the chemical family aldehydes was the most abundant ...201628231176
osnla1, a ring-type ubiquitin ligase, maintains phosphate homeostasis in oryza sativa via degradation of phosphate transporters.inorganic phosphate (pi) transporters (pts) play vital roles in pi uptake and translocation in plants. under pi sufficient conditions, pts are degraded to prevent excess pi accumulation. the mechanisms targeting pts for degradation are not fully elucidated. in this study, we found that the oryza sativa (rice) ortholog of arabidopsis thaliana nitrogen limitation adaptation (nla), osnla1 protein, a ring-type e3 ubiquitin-ligase, was predominantly localized in the plasma membrane, and could interac ...201728229491
genome-wide single-nucleotide polymorphisms in cms and restorer lines discovered by genotyping using sequencing and association with marker-combining ability for 12 yield-related traits in oryza sativa l. subsp. japonica.heterosis or hybrid vigor is closely related with general combing ability (gca) of parents and special combining ability (sca) of combinations. the evaluation of gca and sca facilitate selection of parents and combinations in heterosis breeding. in order to improve combining ability (ca) by molecular marker assist selection, it is necessary to identify marker loci associated with the ca. to identify the single nucleotide polymorphisms (snp) loci associated with ca in the parental genomes of japo ...201728228768
posnar2.1:osnar2.1 expression enhances nitrogen uptake efficiency and grain yield in transgenic rice plants.the nitrate (no3-) transporter has been selected as an important gene maker in the process of environmental adoption in rice cultivars. in this work, we transferred another native osnar2.1 promoter with driving osnar2.1 gene into rice plants. the transgenic lines with exogenous posnar2.1:osnar2.1 constructs showed enhanced osnar2.1 expression level, compared with wild type (wt), and 15 n influx in roots increased 21%-32% in response to 0.2 mm and 2.5 mm 15no3- and 1.25 mm 15 nh415 no3 . under th ...201728226420
endophytic phomopsis sp. colonization in oryza sativa was found to result in plant growth promotion and piperine production.endophytic fungi have been reported to have the acquired ability to synthesize host plant specific medicinal natural products. many fungi with such properties have been characterized and optimized for the conditions which favor maximal production of desired products. however, the inherent plant colonization property of promising endophytic fungi is least studied. exploiting the transgenome functioning of these fungi have immense applications to add beneficial features to non-host plants. in the ...201728224643
the dnaj protein osdja6 negatively regulates rice innate immunity to the blast fungus magnaporthe oryzae.rice blast, caused by magnaporthe oryzae (synonym: pyricularia oryzae), severely reduces rice production and grain quality. the molecular mechanism of rice resistance to m. oryzae is not fully understood. in this study, we identified a chaperone dnaj protein, osdja6, which is involved in basal resistance to m. oryzae in rice. the osdja6 protein is distributed in the entire rice cell. the expression of osdja6 is significantly induced in rice after infection with a compatible isolate. silencing of ...201728220688
de novo transcriptome assembly of phomopsis liquidambari provides insights into genes associated with different lifestyles in rice (oryza sativa l.).the mechanisms that trigger the switch from endophytic fungi to saprophytic fungi are largely unexplored. broad host range phomopsis liquidambari is established in endophytic and saprophytic systems with rice (oryza sativa l.). endophytic p. liquidambari promotes rice growth, increasing rice yield and improving the efficiency of nitrogen fertilizer. this species's saprophytic counterpart can decompose rice litterfall, promoting litter organic matter cycling and the release of nutrients and impro ...201728220138
interference of allelopathic rice with paddy weeds at the root level.despite increasing knowledge of the involvement of allelopathy in negative interactions among plants, relatively little is known about its action at the root level. this study aims to enhance understanding of interactions of roots between a crop and associated weeds via allelopathy. based on a series of experiments with window rhizoboxes and root segregation methods, we examined root placement patterns and root interactions between allelopathic rice and major paddy weeds cyperus difformis, echin ...201728218979
tps46, a rice terpene synthase conferring natural resistance to bird cherry-oat aphid, rhopalosiphum padi (linnaeus).plant terpene synthases (tpss) are key enzymes responsible for terpene biosynthesis, and can play important roles in defense against herbivore attack. in rice, the protein sequence of tps46 was most closely related to maize tps10. however, unlike maize tps10, tps46 was also constitutively expressed in rice even in the absence of herbivore attack. potential roles or constitutive emissions of specific volatiles may due to the constitutive expressions of tps46 in rice. therefore, in the present stu ...201728217135
effect of chemical amendments on remediation of potentially toxic trace elements (ptes) and soil quality improvement in paddy fields.remediation of potentially toxic trace elements (ptes) in paddy fields is fundamental for crop safety. in situ application of chemical amendments has been widely adapted because of its cost-effectiveness and environmental safety. the main purpose of this research was to (1) evaluate the reduction in dissolved concentrations of cadmium (cd) and arsenic (as) with the application of chemical amendments and (2) monitor microbial activity in the soil to determine the remediation efficiency. three dif ...201728213721
bionano genome map resource for oryza sativa ssp. japonica and indica and its application in rice genome sequence correction and gap filling. 201728212831
genetic factors underlying boron toxicity tolerance in rice: genome-wide association study and transcriptomic analysis.boron (b) toxicity is a nutritional disorder affecting crop production in many parts of the world. this study explored genetic factors associated with b tolerance in rice (oryza sativa l.) through an integrated genome mapping and transcriptomic approach. variation in b tolerance was first evaluated by screening a panel of 137 indica genotypes in b toxic conditions (+2 mm b), followed by genome-wide association study (gwas). leaf bronzing and greenness were significantly correlated with shoot and ...201728204664
enzymatic properties of a gh19 chitinase isolated from rice lacking a major loop structure involved in chitin binding.the catalytic domains of family gh19 chitinases have been found to consist of a conserved, α-helical core-region and different numbers (1-6) of loop structures, located at both ends of the substrate-binding groove and which extend over the glycon- and aglycon-binding sites. we expressed, purified and enzymatically characterized a gh19 chitinase from rice, oryza sativa l. cv. nipponbare (oschia2a), lacking a major loop structure (loop iii) connected to the functionally important β-stranded region ...201728204489
cyclic electron flow may provide some protection against psii photoinhibition in rice (oryza sativa l.) leaves under heat stress.previously we have shown that a quick down-regulation in psi activity compares to that of psii following short-term heat stress for two rice groups including c4023 and q4149, studied herein. these accessions were identified to have different natural capacities in driving cyclic electron flow (cef) around psi; i.e., low cef (lcef) and high cef (hcef) for c4023 and q4149, respectively. the aim of this study was to investigate whether these two lines have different mechanisms of protecting photosys ...201728199904
spl33, encoding an eef1a-like protein, negatively regulates cell death and defense responses in rice.lesion-mimic mutants are useful to dissect programmed cell death and defense-related pathways in plants. here we identified a new rice lesion-mimic mutant, spotted leaf 33 (spl33) and cloned the causal gene by a map-based cloning strategy. spl33 encodes a eukaryotic translation elongation factor 1 alpha (eef1a)-like protein consisting of a non-functional zinc finger domain and three functional ef-tu domains. spl33 exhibited programmed cell death-mediated cell death and early leaf senescence, as ...201728199670
toxicity of pesticide tank mixtures from rice crops against telenomus podisi ashmead (hymenoptera: platygastridae).the use of insecticides, herbicides, and fungicides commonly occurs in mixtures in tanks in order to control phytosanitary problems in crops. however, there is no information regarding the effects of these mixtures on non-target organisms associated to the rice agroecosystem. the aim of this study was to know the toxicity of pesticide tank mixtures from rice crops against telenomus podisi ashmead (hymenoptera: platygastridae). based on the methods adapted from the international organisation for ...201728197851
isolation, characterization and transcriptome analysis of a cytokinin receptor mutant osckt1 in rice.cytokinins play important roles in regulating plant development, including shoot and root meristems, leaf longevity, and grain yield. however, the in planta functions of rice cytokinin receptors have not been genetically characterized yet. here we isolated a rice mutant, osckt1, with enhanced tolerance to cytokinin treatment. further analysis showed that osckt1 was insensitive to aromatic cytokinins but responded normally to isoprenoid and phenylurea-type cytokinins. map-based cloning revealed t ...201728197164
fine mapping of carbon assimilation rate 8, a quantitative trait locus for flag leaf nitrogen content, stomatal conductance and photosynthesis in rice.increasing the rate of leaf photosynthesis is one important approach for increasing grain yield in rice (oryza sativa). exploiting the natural variation in co2 assimilation rate (a) between rice cultivars using quantitative genetics is one promising means to identify genes contributing to higher photosynthesis. in this study, we determined precise location of carbon assimilation rate 8 (car8) by crossing a high-yielding indica cultivar with a japanese commercial cultivar. fine mapping suggested ...201728197156
genome-wide characterization of jasmonate-zim domain transcription repressors in wheat (triticum aestivum l.).the jasmonate-zim domain (jaz) repressor family proteins are jasmonate co-receptors and transcriptional repressor in jasmonic acid (ja) signaling pathway, and they play important roles in regulating the growth and development of plants. recently, more and more researches on jaz gene family are reported in many plants. although the genome sequencing of common wheat (triticum aestivum l.) and its relatives is complete, our knowledge about this gene family remains vacant.201728193162
analysis of plant udp-arabinopyranose mutase (uam): role of divalent metals and structure prediction.udp-arabinopyranose mutase (uam) is a plant enzyme which interconverts udp-arabinopyranose (udp-arap; a six-membered sugar) to udp-arabinofuranose (udp-araf; a five-membered sugar). plant mutases belong to a small gene family called reversibly glycosylated proteins (rgps). so far, uam has been identified in oryza sativa (rice), arabidopsis thaliana and hordeum vulgare (barley). the enzyme requires divalent metal ions for catalytic activity. here, the divalent metal ion dependency of uams from o. ...201728192204
genome inside genome: ngs based identification and assembly of endophytic sphingopyxis granuli and pseudomonas aeruginosa genomes from rice genomic reads.the interactions between crop plants and the endophytic bacteria colonizing them are poorly understood and experimental methods were found to be inadequate to meet the complexities associated with the interaction. moreover, research on endophytic bacteria was focused at host plant species level and not at cultivar level which is essential for understanding the role played by them on the productivity of specific crop genotype. high throughput genomics offers valuable tools for identification, cha ...201728192179
genetic variation and host-parasite specificity of striga resistance and tolerance in rice: the need for predictive breeding.the parasitic weeds striga asiatica and striga hermonthica cause devastating yield losses to upland rice in africa. little is known about genetic variation in host resistance and tolerance across rice genotypes, in relation to virulence differences across striga species and ecotypes. diverse rice genotypes were phenotyped for the above traits in s. asiatica- (tanzania) and s. hermonthica-infested fields (kenya and uganda) and under controlled conditions. new rice genotypes with either ecotype-sp ...201728191641
changes in root hydraulic conductivity facilitate the overall hydraulic response of rice (oryza sativa l.) cultivars to salt and osmotic stress.the aim of the present work was to assess the significance of changes in root aqp gene expression and hydraulic conductivity (lp) in the regulation of water balance in two hydroponically-grown rice cultivars (azucena, bala) which differ in root morphology, stomatal regulation and aquaporin (aqp) isoform expression. plants were exposed to nacl (25 mm, 50 mm) and osmotic stress (5%, 10% peg6000). root lp was determined for exuding root systems (osmotic forces driving water uptake; 'exudation lp') ...201728189051
lack of cytosolic glutamine synthetase1;2 activity reduces nitrogen-dependent biosynthesis of cytokinin required for axillary bud outgrowth in rice seedlings.a mutation abolishing cytosolic glutamine synthetase1;2 (gs1;2) activity impairs assimilation of ammonium into glutamine in both roots and basal portions of shoots, and severely decreases axillary bud outgrowth (tillering) in mutant rice seedlings. although the gs1;2 mutant phenotype is independent of strigolactone, which inhibits tillering, it also demonstrates glutamine- or related metabolite-responsive biosynthesis of cytokinin (ck), which promotes tillering. here, we examined the connection ...201728186255
identification of symmetrical rna editing events in the mitochondria of salvia miltiorrhiza by strand-specific rna sequencing.salvia miltiorrhiza is one of the most widely-used medicinal plants. here, we systematically analyzed the rna editing events in its mitochondria. we developed a pipeline using reditools to predict rna editing events from stand-specific rna-seq data. the predictions were validated using reverse transcription, rt-pcr amplification and sanger sequencing experiments. putative sequences motifs were characterized. comparative analyses were carried out between s. miltiorrhiza, arabidopsis thaliana and ...201728186130
marker aided incorporation of saltol, a major qtl associated with seedling stage salt tolerance, into oryza sativa 'pusa basmati 1121'.pusa basmati 1121 (pb1121), an elite basmati rice cultivar is vulnerable to salinity at seedling stage. a study was undertaken to impart seedling-stage salt tolerance into pb1121 by transferring a quantitative trait locus (qtl), saltol, using fl478 as donor, through marker assisted backcrossing. sequence tagged microsatellite site (stms) marker rm 3412, tightly linked to saltol was used for foreground selection. background recovery was estimated using 90 genome-wide stms markers. systematic phen ...201728184228
characterization and ectopic expression of cowri1, an ap2/erebp domain-containing transcription factor from coconut (cocos nucifera l.) endosperm, changes the seeds oil content in transgenic arabidopsis thaliana and rice (oryza sativa l.).coconut (cocos nucifera l.) is a key tropical crop and a member of the monocotyledonous family arecaceae (palmaceae). few genes and related metabolic processes involved in coconut endosperm development have been investigated. in this study, a new member of the wri1 gene family was isolated from coconut endosperm and was named cowri1. its transcriptional activities and interactions with the acetyl-coa carboxylase (bccp2) promoter of cowri1 were confirmed by the yeast two-hybrid and yeast one-hybr ...201728179911
genome-wide identification and analysis of biotic and abiotic stress regulation of small heat shock protein (hsp20) family genes in bread wheat.small heat shock proteins (shsps)/hsp20 are molecular chaperones that protect plants by preventing protein aggregation during abiotic stress conditions, especially heat stress. due to global climate change, high temperature is emerging as a major threat to wheat productivity. thus, the identification of hsp20 and analysis of hsp transcriptional regulation under different abiotic stresses in wheat would help in understanding the role of these proteins in abiotic stress tolerance. we used sequence ...201728178571
rapid identification of a new gene influencing low amylose content in rice landraces (oryza sativa l.) using genome-wide association study with specific-locus amplified fragment sequencing.starch is the major component of milled rice, and amylose content (ac) affects eating quality. in this study, a genome-wide association study (gwas) with specific-locus amplified fragment sequencing (slaf-seq) data was performed for ac on a core collection of 419 rice landraces. using the compressed mixed linear method based on the q+k model, we identified a new gene, lac6 (chr6: 5.65-6.04 mb), associated with ac in the low amylose content panel. the lac6 candidate gene was detected by qrt-pcr i ...201728177831
evolutionary analysis of the sub1 locus across the oryza genomes.tolerance to complete submergence is recognized in a limited number of asian rice (oryza sativa l.) varieties, most of which contain submergence-inducible sub1a on the polygenic submergence-1 (sub1) locus. it has been shown that the sub1 locus encodes two ethylene-responsive factor (erf) genes, sub1b and sub1c, in all o. sativa varieties. these genes were also found in o rufipogon and o nivara, wild relatives of o. sativa. however, detailed analysis of the polygenic locus in other oryza species ...201728176282
origin and diversification of leucine-rich repeat receptor-like protein kinase (lrr-rlk) genes in plants.leucine-rich repeat receptor-like protein kinases (lrr-rlks) are the largest group of receptor-like kinases in plants and play crucial roles in development and stress responses. the evolutionary relationships among lrr-rlk genes have been investigated in flowering plants; however, no comprehensive studies have been performed for these genes in more ancestral groups. the subfamily classification of lrr-rlk genes in plants, the evolutionary history and driving force for the evolution of each lrr-r ...201728173747
interactions between nitrogen and silicon in rice and their effects on resistance toward the brown planthopper nilaparvata lugens.nitrogen (n) and silicon (si) are two important nutritional elements required for plant growth, and both impact host plant resistance toward insect herbivores. the interaction between the two elements may therefore play a significant role in determining host plant resistance. we investigated this interaction in rice (oryza sativa l.) and its effect on resistance to the herbivore brown planthopper nilaparvata lugens (bph). our results indicate that high-level (5.76 mm) n fertilization reduced si ...201728167952
newly identified csp41b gene localized in chloroplasts affects leaf color in rice.a rice mutant with light-green leaves was discovered from a transgenic line of oryza sativa. the mutant has reduced chlorophyll content and abnormal chloroplast morphology throughout its life cycle. genetic analysis revealed that a single nuclear-encoded recessive gene is responsible for the mutation, here designated as lgl1. to isolate the lgl1 gene, a high-resolution physical map of the chromosomal region around the lgl1 gene was made using a mapping population consisting of 1984 mutant indivi ...201728167036
development of an inducible male-sterility system in rice through pollen-specific expression of l-ornithinase (arge) gene of e. coli.in the present investigation, an inducible male-sterility system has been developed in the rice. in order to introduce inducible male-sterility, the coding region of l-ornithinase (arge) gene of e. coli was fused to the oryza sativa indica pollen allergen (osipa) promoter sequence which is known to function specifically in the pollen grains. transgenic plants were obtained with arge gene and the transgenic status of plants was confirmed by pcr and southern blot analyses. rt-pcr analysis confirme ...201728167027
homologous u-box e3 ubiquitin ligases ospub2 and ospub3 are involved in the positive regulation of low temperature stress response in rice (oryza sativa l.).rice u-box e3 ub ligases (ospubs) are implicated in biotic stress responses. however, their cellular roles in response to abiotic stress are poorly understood. in this study, we performed functional analyses of two homologous ospub2 and ospub3 in response to cold stress (4°c). ospub2 was up-regulated by high salinity, drought, and cold, whereas ospub3 was constitutively expressed. a subcellular localization assay revealed that ospub2 and ospub3 were localized to the exocyst positive organelle (e ...201728163713
identification of a low tiller gene from a new plant type cultivar in rice (oryza sativa l.).we characterized a rice introgression line, yth34, harboring a chromosome segment from a new plant type (npt) cultivar, ir65600-87-2-2-3, in the genetic background of an indica group elite rice cultivar, ir 64, under upland and irrigated lowland conditions in japan. the number of panicles (as an indicator of tiller number) and number of spikelets per panicle of yth34 were lower than those of ir 64 under irrigated lowland conditions, but both of those as well as culm length, panicle length, seed ...201628163595
distribution of hwc2-1, a causal gene of a hybrid weakness, in the world rice core collection and the japanese rice mini core collection: its implications for varietal differentiation and artificial selection.a pair of complementary genes, hwc1-1 at hwc1 locus and hwc2-1 at hwc2 locus, cause a weakness phenomenon in rice. for this study, we performed haplotype analysis around the hwc2 locus in two core collections comprising 119 accessions. we also examined reactions to phenol and xanthomonas oryzae pv. oryzae (xoo) japanese race i. to elucidate the genetic relations among all accessions, we analyzed their banding patterns of 40 indel markers covering the rice genome. the classification by indel mark ...201628163594
development of a platform for breeding by design of cms restorer lines based on an sssl library in rice (oryza sativa l.).exploitation of the heterosis of hybrid rice has shown great success in the improvement of rice yields. however, few genotypes exhibit strong restoration ability as effective restorers of cytoplasmic male sterility (cms) in the development of hybrid rice. in this study, we developed a platform for the breeding by design of cms restorer lines based on a library of chromosomal single segment substitution lines (sssls) in the huajingxian74 (hjx74) genetic background. the target genes for breeding b ...201628163593
genetic variation of rice (oryza sativa l.) germplasm in myanmar based on genomic compositions of dna markers.the genetic diversity of 175 rice accessions from myanmar, including landraces and improved types from upland and lowland ecosystems in five different areas-western (hilly), northern (mountainous), north and south-eastern (plateau), and southern (plain)-was evaluated on the basis of polymorphism data for 65 dna markers and phenol reactions. on the basis of the dna polymorphism data, high genetic diversity was confirmed to conserve in the accessions from each ecosystem and area. and the accession ...201628163592
phylogenomic analysis of gene co-expression networks reveals the evolution of functional modules.molecular evolutionary studies correlate genomic and phylogenetic information with the emergence of new traits of organisms. these traits are, however, the consequence of dynamic gene networks composed of functional modules, which might not be captured by genomic analyses. here, we established a method that combines large-scale genomic and phylogenetic data with gene co-expression networks to extensively study the evolutionary make-up of modules in the moss physcomitrella patens, and in the angi ...201728161902
a small decrease in rubisco content by individual suppression of rbcs genes leads to improvement of photosynthesis and greater biomass production in rice under conditions of elevated co2.rubisco limits photosynthesis at low co2 concentrations ([co2]), but does not limit it at elevated [co2]. this means that the amount of rubisco is excessive for photosynthesis at elevated [co2]. therefore, we examined whether a small decrease in rubisco content by individual suppression of the rbcs multigene family leads to increases in photosynthesis and biomass production at elevated [co2] in rice (oryza sativa l.). our previous studies indicated that the individual suppression of rbcs decreas ...201728158810
plantexpress: a database integrating oryzaexpress and arthaexpress for single-species and cross-species gene expression network analyses with microarray-based transcriptome data.publicly available microarray-based transcriptome data on plants are remarkably valuable in terms of abundance and variation of samples, particularly for oryza sativa (rice) and arabidopsis thaliana (arabidopsis). here, we introduce the web database plantexpress (http://plantomics.mind.meiji.ac.jp/plantexpress/) as a platform for gene expression network (gen) analysis with the public microarray data of rice and arabidopsis. plantexpress has two functional modes. the single-species mode is specia ...201728158643
a phosphoinositide-specific phospholipase c pathway elicits stress-induced ca2+ signals and confers salt tolerance to rice.in animal cells, phospholipase c (plc) isoforms predominantly hydrolyze phosphatidylinositol-4,5-biphosphates [ptdins(4,5)p2 ] into the second messengers diacylglycerol (dag) and inositol 1,4,5-trisphosphate [ins(1,4,5)p3 ] to regulate diverse biological processes. by contrast, the molecular mechanisms and physiological significance of plc signaling in plants still awaits full elucidation. here, we identified a rice (oryza sativa cv) pi-plc, osplc1, which preferred to hydrolyze phosphatidylinosi ...201728157263
in situ evaluation of crop productivity and bioaccumulation of heavy metals in paddy soils after remediation of metal-contaminated soils.soils contaminated with heavy metals have been reused for agricultural, building, and industrial uses following remediation. this study assesses plant growth and bioaccumulation of heavy metals following remediation of industrially contaminated soil. the soil was collected from a field site near a nonferrous smelter and was subjected to laboratory- and field-scale studies. soil from the contaminated site was remediated by washing with acid or mixed with soil taken from a distant uncontaminated s ...201728150951
fine mapping of rymv3: a new resistance gene to rice yellow mottle virus from oryza glaberrima.a new resistance gene against rice yellow mottle virus was identified and mapped in a 15-kb interval. the best candidate is a cc-nbs-lrr gene. rice yellow mottle virus (rymv) disease is a serious constraint to the cultivation of rice in africa and selection for resistance is considered to be the most effective management strategy. the aim of this study was to characterize the resistance of tog5307, a highly resistant accession belonging to the african cultivated rice species (oryza glaberrima), ...201728144699
absence of osβca1 causes a co2 deficit and affects leaf photosynthesis and the stomatal response to co2 in rice.plants always adjust the opening of stomatal pores to adapt to the environment, for example co2 concentration ([co2 ]), humidity and temperature. low [co2 ] will trigger the opening of stomatal pores to absorb extra co2 . however, little is known about how co2 supply affects the carbon fixation and opening of stomatal pores in rice. here, a chloroplast-located gene coding for β-carbonic anhydrase (βca) was found to be involved in carbon assimilation and the co2 -mediated stomatal pore response i ...201728142196
ascorbic acid deficiency leads to increased grain chalkiness in transgenic rice for suppressed of l-galldh.the grain chalkiness of rice (oryza sativa l.), which determines the rice quality and price, is a major concern in rice breeding. reactive oxygen species (ros) plays a critical role in regulating rice endosperm chalkiness. ascorbic acid (asc) is a major plant antioxidant, which strictly regulates the levels of ros. l-galactono-1, 4-lactone dehydrogenase (l-galldh, ec 1.3.2.3) is an enzyme that catalyzes the last step of asc biosynthesis in higher plants. here we show that the l-galldh-suppressed ...201728142093
the local impact of a coal-fired power plant on inorganic mercury and methyl-mercury distribution in rice (oryza sativa l.).emission from coal-fired power plants is one of the major anthropogenic sources of mercury (hg) in the environment, because emitted hg can be quickly deposited nearby the source, attention is paid to the effects of coal-burning facilities on levels of toxic methyl-mercury (mehg) in biota near such sources. since rice is an agricultural crop that can bio-accumulate mehg, the potential effects of a large hg-emitting coal-fired power plant in hunan province, china on both inorganic hg (hg(ii)) and ...201728139322
synergistic effects between [si-hemicellulose matrix] ligands and zn ions in inhibiting cd ion uptake in rice (oryza sativa) cells.our study demonstrated that zn alleviated cd toxicity in the presence of si in the cell walls by zn 2+ binding to ligands through the formation of the [si-hemicellulose matrix]zn complexes that restrict the uptake of cd. the plant cell wall exhibits preferential sites for the accumulation of metals at toxic concentrations. through modification of wall polysaccharide components, elements, such as silicon (si) and zinc (zn), may play active roles in alleviating the toxicity of heavy metals, includ ...201728138761
biochemical and chemical biology study of rice ostar1 revealed that tryptophan aminotransferase is involved in auxin biosynthesis: identification of a potent ostar1 inhibitor, pyruvamine2031.iaa, a major form of auxin, is biosynthesized from l-tryptophan via the indole-3-pyruvic acid (ipya) pathway in arabidopsis. tryptophan aminotransferases (taa1/tars) catalyze the first step from l-tryptophan to ipya. in rice, the importance of taa/tars or yuc homologs in auxin biosynthesis has been suggested, but the enzymatic activities and involvement of the intermediate ipya in auxin biosynthesis remain elusive. in this study, we obtained biochemical evidence that the rice tryptophan aminotra ...201728138057
identification and characterization of a plastidic adenine nucleotide uniporter (osbt1-3) required for chloroplast development in the early leaf stage of rice.chloroplast development is an important subject in botany. in this study, a rice (oryza sativa) mutant exhibiting impairment in early chloroplast development (seedling leaf albino (sla)) was isolated from a filial generation via hybridization breeding. the sla mutant seedlings have an aberrant form of chloroplasts, which resulted in albinism at the first and second leaves; however, the leaf sheath was green. the mutant gradually turned green after the two-leaf stage, and the third leaf was a nor ...201728134341
genetic analysis and identification of ssr markers associated with rice blast disease in a bc2f1 backcross population.rice (oryza sativa l.) blast disease is one of the most destructive rice diseases in the world. the fungal pathogen, magnaporthe oryzae, is the causal agent of rice blast disease. development of resistant cultivars is the most preferred method to achieve sustainable rice production. however, the effectiveness of resistant cultivars is hindered by the genetic plasticity of the pathogen genome. therefore, information on genetic resistance and virulence stability are vital to increase our understan ...201728128411
a salivary endo-β-1,4-glucanase acts as an effector that enables the brown planthopper to feed on rice.the brown planthopper (bph) nilaparvata lugens is one of the most destructive insect pests on rice (oryza sativa) in asia. after landing on plants, bph rapidly accesses plant phloem and sucks the phloem sap through unknown mechanisms. we discovered a salivary endo-β-1,4-glucanase (nleg1) that has endoglucanase activity with a maximal activity at ph 6 at 37°c and is secreted into rice plants by bph nleg1 is highly expressed in the salivary glands and midgut. silencing nleg1 decreases the capacity ...201728126846
physiological analysis of brassinosteroid responses and sensitivity in rice.identification of related mutants contributed a lot to our understanding of the molecular mechanisms underlying brassinosteroid (br) signaling. establishment of methods to test for the hormone sensitivity is essential for the characterization of the mutants associated with br responses. here, we describe detailed procedures for the physiological analysis of br responses that are regularly applied to test the br sensitivity of mutants by evaluating the growth of different tissues of rice (oryza s ...201728124243
non-photochemical quenching plays a key role in light acclimation of rice plants differing in leaf color.non-photochemical quenching (npq) is an important photoprotective mechanism in rice; however, little is known regarding its role in the photosynthetic response of rice plants with differing in leaf color to different irradiances. in this study, two rice genotypes containing different chlorophyll contents, namely zhefu802 (high chlorophyll) and chl-8 (low chlorophyll), were subjected to moderate or high levels of light intensity at the 6-leaf stage. chl-8 possessed a lower chlorophyll content and ...201628119700
overexpression of rice serotonin n-acetyltransferase 1 in transgenic rice plants confers resistance to cadmium and senescence and increases grain yield.while ectopic overexpression of serotonin n-acetyltransferase (snat) in plants has been accomplished using animal snat genes, ectopic overexpression of plant snat genes in plants has not been investigated. because the plant snat protein differs from that of animals in its subcellular localization and enzyme kinetics, its ectopic overexpression in plants would be expected to give outcomes distinct from those observed from overexpression of animal snat genes in transgenic plants. consistent with o ...201728118490
oscesa9 conserved-site mutation leads to largely enhanced plant lodging resistance and biomass enzymatic saccharification by reducing cellulose dp and crystallinity in rice.genetic modification of plant cell walls has been posed to reduce lignocellulose recalcitrance for enhancing biomass saccharification. since cellulose synthase (cesa) gene was first identified, several dozen cesa mutants have been reported, but almost all mutants exhibit the defective phenotypes in plant growth and development. in this study, the rice (oryza sativa) osfc16 mutant with substitutions (w481c, p482s) at p-cr conserved site in cesa9 shows a slightly affected plant growth and higher b ...201728117552
the relationship between vacuolation and initiation of pcd in rice (oryza sativa) aleurone cells.vacuole fusion is a necessary process for the establishment of a large central vacuole, which is the central location of various hydrolytic enzymes and other factors involved in death at the beginning of plant programmed cell death (pcd). in our report, the fusion of vacuoles has been presented in two ways: i) small vacuoles coalesce to form larger vacuoles through membrane fusion, and ii) larger vacuoles combine with small vacuoles when small vacuoles embed into larger vacuoles. regardless of h ...201728117452
the effect of iron plaque on uptake and translocation of norfloxacin in rice seedlings grown in paddy soil.although the role of iron plaque on rice root surface has been investigated in recent years, its effect on antibiotic uptake remains uncertain. in the study, pot experiment was conducted to investigate the effect of iron plaque on uptake and translocation of norfloxacin (adding 10 and 50 mg·kg-1 treatments) in rice seedlings grown in paddy soil. iron plaque was induced by adding different amounts of fe(ii) in soil. the results showed that the presence of norfloxacin can decrease the amount of ir ...201728116626
cultivars effect on the physical characteristics of rice (rough and milled) (oryza sativa l.) of temperate region of kashmir (india).the aim of present research was to evaluate physical and engineering properties of traditional paddy and rice cultivars native to temperate region of india. length, width, thickness, equivalent diameter, surface area, aspect ratio, volume, bulk density, true density, porosity, thousand kernels weight, angle of repose and coefficient of friction were evaluated, which are required in designing of various post harvest operations and storage structures. the low bulk density of cultivars, mushki budg ...201628115766
oscerk1-mediated chitin perception and immune signaling requires receptor-like cytoplasmic kinase 185 to activate an mapk cascade in rice.conserved pathogen-associated molecular patterns (pamps), such as chitin, are perceived by pattern recognition receptors (prrs) located at the host cell surface and trigger rapid activation of mitogen-activated protein kinase (mapk) cascades, which are required for plant resistance to pathogens. however, the direct links from pamp perception to mapk activation in plants remain largely unknown. in this study, we found that the prr-associated receptor-like cytoplasmic kinase oryza sativa rlck185 t ...201728111288
ability of rf5 and rf6 to restore fertility of chinsurah boro ii-type cytoplasmic male sterile oryza sativa (ssp. japonica) lines.three-line oryza sativa (ssp. japonica) hybrids have been developed mainly using chinsurah boro ii (bt)-type cytoplasmic male sterility (cms). the rf1 gene restores the fertility of bt-type cms lines, and is the only fertility restorer gene (rf) that has been used to produce three-line japonica hybrids. using more rf genes to breed bt-type restorer lines may broaden the genetic diversity of the restorer lines, and represents a viable approach to improve the heterosis level of bt-type japonica hy ...201728110460
genome-wide association analyses reveal the genetic basis of stigma exsertion in rice.stigma exsertion, a key determinant of the rice mating system, greatly contributes to the application of heterosis in rice. although a few quantitative trait loci associated with stigma exsertion have been fine mapped or cloned, the underlying genetic architecture remains unclear. we performed a genome-wide association study on stigma exsertion and related floral traits using 6.5 million snps characterized in 533 diverse accessions of oryza sativa. we identified 23 genomic loci that are signific ...201728110091
quantitative classification of rice (oryza sativa l.) root length and diameter using image analysis.quantitative study of root morphological characteristics of plants is helpful for understanding the relationships between their morphology and function. however, few studies and little detailed and accurate information of root characteristics were reported in fine-rooted plants like rice (oryza sativa l.). the aims of this study were to quantitatively classify fine lateral roots (flrs), thick lateral roots (tlrs), and nodal roots (nrs) and analyze their dynamics of mean diameter (md), lengths an ...201728103264
foliar uptake of radiocaesium from irrigation water by paddy rice (oryza sativa): an overlooked pathway in contaminated environments.flooded (paddy) rice (oryza sativa) can take up ions from the irrigation water by foliar uptake via the exposed stem base. we hypothesised that the stem base uptake of radiocaesium (rcs) is a pathway for rice grown in rcs-contaminated environments. we developed a bi-compartmental device which discriminates the stem base from root rcs uptake from solutions, thereby using rcs isotopes (137 cs and 134 cs) with < 2% solution leak between the compartments. radiocaesium uptake was linear over time (0- ...201728102551
variation in accumulation and translocation of di-n-butyl phthalate (dbp) among rice (oryza sativa l.) genotypes and selection of cultivars for low dbp exposure.di-n-butyl phthalate (dbp) is a typical endocrine-disrupting chemical with higher detection frequency and concentration in agricultural soil (particularly in paddy-field soil of guangdong province) of china. in this study, a greenhouse experiment was conducted to investigate variation in uptake and accumulation of dbp by 20 rice cultivars and to screen low dbp-accumulating cultivars. dbp concentrations in plants varied greatly with rice cultivars, growth stages, and tissues. the highest dbp conc ...201728102498
the rla1/smos1 transcription factor functions with osbzr1 to regulate brassinosteroid signaling and rice architecture.brassinosteroids (brs) are plant-specific steroid hormones that control plant growth and development. recent studies have identified key components of the br signaling pathway in arabidopsis thaliana and in rice (oryza sativa); however, the mechanism of br signaling in rice, especially downstream of gsk3/shaggy-like kinase (gsk2), remains unclear. here, we identified a br-insensitive rice mutant, reduced leaf angle1 (rla1), and cloned the corresponding gene. rla1 was identical to the previously ...201728100707
oscul3a negatively regulates cell death and immunity by degrading osnpr1 in rice.cullin3-based ring e3 ubiquitin ligases (crl3), composed of cullin3 (cul3), rbx1, and btb proteins, are involved in plant immunity, but the function of cul3 in the process is largely unknown. here, we show that rice (oryza sativa) oscul3a is important for the regulation of cell death and immunity. the rice lesion mimic mutant oscul3a displays a significant increase in the accumulation of flg22- and chitin-induced reactive oxygen species, and in pathogenesis-related gene expression as well as res ...201728100706
pathogenicity genes in ustilaginoidea virens revealed by a predicted protein-protein interaction network.rice false smut, caused by ustilaginoidea virens, produces significant losses in rice yield and grain quality and has recently emerged as one of the most important rice diseases worldwide. despite its importance in rice production, relatively few studies have been conducted to illustrate the complex interactome and the pathogenicity gene interactions. here a protein-protein interaction network of u. virens was built through two well-recognized approaches, interolog- and domain-domain interaction ...201728099032
reactive oxygen species generated by nadph oxidases promote radicle protrusion and root elongation during rice seed germination.seed germination is a complicated biological process that requires regulation through various enzymatic and non-enzymatic mechanisms. although it has been recognized that reactive oxygen species (ros) regulate radicle emergence and root elongation in a non-enzymatic manner during dicot seed germination, the role of ros in monocot seed germination remains unknown. nadph oxidases (noxs) are the major ros producers in plants; however, whether and how noxs regulate rice seed germination through ros ...028098759
different physiobiochemical and transcriptomic reactions of rice (oryza sativa l.) cultivars differing in terms of salt sensitivity under salinity stress.salinity stress is the most important and common environmental stresses throughout the world, including iran. the aim of this study was to investigate the expression of several important genes involved in the salinity tolerance of the rice cultivars differing in salt sensitivity. in this research, the expression of four mitochondrial genes, h2o2, malondialdehyde (mda), proline, sodium, potassium and superoxide dismutase (sod), was measured in iranian rice cultivars and two well-known internation ...201728097484
the calcium-dependent protein kinase (cdpk) and cdpk-related kinase gene families in hevea brasiliensis-comparison with five other plant species in structure, evolution, and expression.calcium-dependent protein kinases (cdpks or cpks) play important roles in various physiological processes of plants, including growth and development, stress responses and hormone signaling. although the cdpk gene family has been characterized in several model plants, little is known about this gene family in hevea brasiliensis (the para rubber tree). here, we characterize the entire h. brasiliensis cdpk and cdpk-related kinase (crk) gene families comprising 30 cdpk genes (hbcpk1 to 30) and nine ...201728097084
flowering time regulation by the constans-like gene oscol10.plant flowering at the appropriate time is critical for reproductive success and influenced by a series of environmental factors such as photoperiod and temperature. a number of genes involved in photoperiodic flowering of have been cloned and their roles in modulating expression of the flowering genes have been characterized to a certain extent. however, much less is known about the pathway in transmitting the day length response signal(s) to induce transition to reproductive growth. recently, ...201728095114
proteomic and carbonylation profile analysis at the critical node of seed ageing in oryza sativa.the critical node (cn), which is the transition from the plateau phase to the rapid decreasing phase of seed ageing, is extremely important for seed conservation. although numerous studies have investigated the oxidative stress during seed ageing, information on the changes in protein abundance at the cn is limited. in this study, we aimed to investigate the abundance and carbonylation patterns of proteins at the cn of seed ageing in rice. the results showed that the germination rate of seeds de ...201728094349
accumulation and transformation of inorganic and organic arsenic in rice and role of thiol-complexation to restrict their translocation to shoot.environmental contamination of arsenic (as) and its accumulation in rice (oryza sativa l.) is of serious human health concern. in planta speciation of as is an important tool to understand as metabolism in plants. in the present study, we investigated root to shoot as translocation and speciation in rice exposed to inorganic and methylated as. arsenate (asv) and methylarsonate (mav) were efficiently reduced to arsenite (asiii) and maiii, respectively in rice root and shoot but no trivalent form ...201728094280
evaluation of hyperspectral lidar for monitoring rice leaf nitrogen by comparison with multispectral lidar and passive spectrometer.fast and nondestructive assessment of leaf nitrogen concentration (lnc) is critical for crop growth diagnosis and nitrogen management guidance. in the last decade, multispectral lidar (msl) systems have promoted developments in the earth and ecological sciences with the additional spectral information. with more wavelengths than msl, the hyperspectral lidar (hsl) system provides greater possibilities for remote sensing crop physiological conditions. this study compared the performance of asd fie ...201728091610
dark septate endophyte decreases stress on rice plants.abiotic stress is one of the major limiting factors for plant development and productivity, which makes it important to identify microorganisms capable of increasing plant tolerance to stress. dark septate endophytes can be symbionts of plants. in the present study, we evaluated the ability of dark septate endophytes isolates to reduce the effects of water stress in the rice varieties nipponbare and piauí. the experiments were performed under gnotobiotic conditions, and the water stress was indu ...201728089614
recognition of the magnaporthe oryzae effector avr-pia by the decoy domain of the rice nlr immune receptor rga5.nucleotide binding domain and leucine-rich repeat proteins (nlrs) are important receptors in plant immunity that allow recognition of pathogen effectors. the rice (oryza sativa) nlr rga5 recognizes the magnaporthe oryzae effector avr-pia through direct interaction. here, we gained detailed insights into the molecular and structural bases of avr-pia-rga5 interaction and the role of the ratx1 decoy domain of rga5. nmr titration combined with in vitro and in vivo protein-protein interaction analyse ...201728087830
tracheophytes contain conserved orthologs of a basic helix-loop-helix transcription factor that modulate root hair specific genes.root hair specific (rhs) genes, which contain the root hair-specific cis-element (rhe) in their regulatory regions, function in root hair morphogenesis. here, we demonstrate that an arabidopsis thaliana basic helix-loop-helix transcription factor, root hair defectve six-like4 (rsl4), directly binds to the rhe in vitro and in vivo, upregulates rhs genes, and stimulates root hair formation in arabidopsis. orthologs of rsl4 from a eudicot (poplar [populus trichocarpa]), a monocot (rice [oryza sativ ...201728087829
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