Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| specific detection of xylella fastidiosa pierce's disease strains. | pierce's disease (pd, xylella fastidiosa) of grapevine is the primary pathogen limiting vinifera grape production in florida and other regions of the southeastern united states. quick and accurate detection of pd strains is essential for pd studies and control. a unique random amplified polymorphic dna (pd1-1-2) was isolated from a pd strain from florida. fragment pd1-1-2 was cloned, sequenced, and found to be 1005 bp in length. pcr primers were designed to utilize these sequence data for pd str ... | 1999 | 10398832 |
| proceedings of the middle european meeting on plum pox. smolenice, slovakia, june 29-july 3, 1998. | 1998 | 10391808 | |
| host status of fresh prunes by potential quarantine pests in laboratory tests and evaluation of packinghouse culls. | the status of fresh prunes, prunus domestica l., as a host for codling moth, cydia pomonella (l.) (lepidoptera: tortricidae); peach twig borer, anarsia lineatella zeller (lepidoptera: gelechiidae); omnivorous leafroller, platynota stultana walshingham (lepidoptera: tortricidae); oriental fruit moth, grapholita molesta (busck) (lepidoptera: tortricidae); navel orangeworm, amyelois transitella (walker) (lepidoptera: pyralidae); and walnut husk fly, rhagoletis completa cresson (diptera: tephritidae ... | 1999 | 10333755 |
| rna helicase activity of semliki forest virus replicase protein nsp2. | semliki forest virus replicase protein nsp2 shares sequence homology with several putative ntpases and rna helicases. nsp2 has rna-dependent ntpase activity. here we expressed polyhistidine-tagged nsp2 in escherichia coli, purified it by metal-affinity chromatography, and used it in rna helicase assays. rna helicase ci of plum pox potyvirus was used as a positive control. unwinding of alpha-32p-labelled partially double-stranded rna required nsp2, mg2+ and ntps. nsp2 with a mutation, k192n, in t ... | 1999 | 10217401 |
| high pressure response of fruit jams contaminated with listeria monocytogenes. | high pressure is an alternative to thermal processing and is used to preserve food. listeria monocytogenes is a bacterium which grows at low temperature, is able to multiply under vacuum, and is responsible for food poisoning. pressures of 100, 200, 300 and 400 mpa were used for 5, 10 and 15 min at 20 degrees c on pure culture, and on apple and plum jam baby food artificially contaminated with listeria. pure culture was also to test pressures of 200, 300, 350 and 400 mpa at 5 degrees c for 30 mi ... | 1999 | 10212445 |
| marissa and alyssa--new names in transplantation frontiers. | as the national media focused attention on teenager anissa ayala who received a transplant of marrow cells "donated" by her baby sister, marissa, who was conceived for the purpose of providing the marrow, a less publicized unsuccessful transplant procedure performed on alyssa plum a month earlier probably poses a greater challenge to ethical analysis. the following articles describe both stories. | 1991 | 10111505 |
| nailing down construction costs. design-build approach may have some answers. | design-build firms, long accustomed to getting the smaller projects in the healthcare construction industry, have reason to be optimistic that the plum jobs will no longer be out of reach. at a time when the healthcare industry is under intense pressure to cut costs, but the need for renovation and new construction is increasing, design-build is a construction approach that promises to save time and money and guarantees price up front. as an alternative to traditional design-bid-build constructi ... | 1990 | 10104649 |
| detection of plum pox virus by enzyme-linked immunosorbent assay in some apricot and peach varieties and hybrids in romania. | plum pox virus (ppv) is a potyvirus widely spread in many species of the prunus genus such as plum, apricot, peach, sweet cherry and others. this potyvirus causes great damage to stone fruit trees in romania and other european countries as hungary, italy, czech republic, france, spain, greece, turkey, and slovak republic. the research station for fruit tree growing baneasa in bucharest has realized many studies on the epidemiology and spread of ppv and also on the disease symptomatology and dete ... | 1998 | 10073239 |
| agroclimatical analysis of plum pox virus spread in slovakia. | in this report, the plum pox virus (ppv) spread from the point of view of agroclimatical conditions on the territory of slovakia is analysed. the worst condition for the ppv spread was found in a warm macroregion. the air temperature sum in this macroregion during vegetative period ranged from 2400 to 3100 degrees c. the worst condition for the ppv spread was found in dry subregions of the macroregion. these were defined by differences between potential evapotranspiration and rainfall sums which ... | 1998 | 10073238 |
| characterization of phenotype resistance to plum pox of transgenic plums expressing plum pox virus capsid gene. | resistance to plum pox virus (ppv) infection can be obtained in transgenic plants that express the virus capsid gene. an agrobacterium-mediated transformation was used to introduce the ppv capsid gene into prunus domestica plants. over 11 regenerated plants (clones) were observed for the development of the disease symptoms and analysed for the presence of ppv by enzyme-linked immunosorbent assay (elisa), western blot analysis, and reverse transcription-polymerase chain reaction (rt-pcr) through ... | 1998 | 10073237 |
| restriction fragment length polymorphism differentiation of plum pox virus isolates. | reverse transcription-polymerase chain reaction (rt-pcr) technique and restriction fragment length polymorphism (rflp) analysis were used to analyse six isolates of plum pox virus (ppv). whole coat protein (cp) gene was amplified in four isolates using the unipoty-polyt primer pari. ppv-d was identified by rflp analysis using sfui and drai enzymes in two of the isolates. two isolates of ppv-m strain yielded rt-pcr products which could not be digested by the two enzymes. other isolates were subje ... | 1998 | 10073236 |
| relative concentration of plum pox virus in leaves and flowers of some prunus species and cultivars. | the relative concentration of plum pox virus (ppv) in leaves and flowers of plum, damson, myrobalan, blackthorn, apricot and peach trees was determined by enzyme-linked immunosorbent assay (elisa) and expressed as the lowest dilution with positive reaction. significant differences in relative ppv concentration were found in leaves among individual prunus species naturally or artificially infected with the virus. the highest relative ppv concentration was found in blackthorns (7.81 x 10(-4)), com ... | 1998 | 10073235 |
| detection of plum pox virus in apricot seeds. | twelve different apricot selection trees from a germplasm collection naturally infected with plum pox virus (ppv) were chosen to investigate the role of seeds in the epidemiology of this dangerous pathogen. all the considered plants showed typical symptoms on leaves and fruits and were positive in enzyme-linked immunosorbent assay (elisa). the virus was characterized by immunocapture reverse transcription-polymerase chain reaction (ic-rt-pcr) followed by restriction fragment length polymorphism ... | 1998 | 10073234 |
| testing of plum germplasm for sensitivity to plum pox. | a long-term orchard experiment with a broad assortment of plum cultivars aimed to screen their sensitivity to plum pox virus (ppv) was established in 1991. for this purpose, 207 cultivars to be artificially infected with ppv at a permanent site were chosen. the serotype m of ppv from a tree of cv. domestic prune, which had not been contaminated by other viruses, was used as a source of the infection. three buds infected with ppv were budded on 1-year-old trees. in the course of experiment the fo ... | 1998 | 10073233 |
| molecular variability of czech plum pox virus isolates. | seventy-two czech plum pox virus (ppv) isolates from different hosts were tested by double-antibody sandwich enzyme-linked immunosorbent assay (das-elisa). in addition, the coat protein mobility and the rsai restriction fragment length polymorphism (rflp) pattern of reverse transcription-polymerase chain reaction-(rt-pcr) amplified coat protein (cp) gene fragment of the isolates were analysed. both ppv-d and ppv-m serotypes were found in the czech republic. the results obtained by these differen ... | 1998 | 10073232 |
| detection and serotyping of mediterranean plum pox virus isolates by means of strain-specific monoclonal antibodies. | plum pox virus (ppv) is a major threat to the expanding mediterranean stone fruit industry. in order to control the plum pox disease it is of utmost importance to detect early ppv foci and to identify the ppv isolates involved. a survey was therefore carried out in albania, cyprus, egypt, greece, italy and turkey by a double-antibody sandwich enzyme-linked immunosorbent assay (das-elisa) with the following monoclonal antibodies (mabs): 5b (universal), 4dg5 (ppv-d-specific), al (ppv-m-specific), ... | 1998 | 10073231 |
| production of a monoclonal antibody specific to the el amar strain of plum pox virus. | plum pox virus (ppv) isolates are grouped into three clusters differentiated by biological, serological, molecular and epidemiological characteristics: marcus (m), dideron (d) and cherry (c). the el amar (ea) isolate that does not fit any of the above groups is also known. monoclonal antibodies (mabs) that specifically recognize m, d, and c strains of ppv are already available. to complete the set of ppv strain-specific serological reagents, mabs against the ea isolate were raised by immunizing ... | 1998 | 10073230 |
| new results concerning the plum pox virus epidemiology and resistance of plum cultivars, hybrids and rootstocks. | our recent results on the plum pox virus (ppv) epidemiology show that ppv spreads very rapidly in plum tree plantations in the contaminated areas. a clearing of the ppv-infected trees reduces significantly the spread of the virus but does not eliminate the disease. some plum tree cultivars, hybrids and rootstocks (scoldus, alina, cristi, bn 1/8fl, bn 2gr. etc) showing field resistance could not be infected with ppv by natural way. however, they could be infected with ppv by artificial inoculatio ... | 1998 | 10073229 |
| preliminary report on the apparent breaking of resistance of a transgenic plum by chip bud inoculation of plum pox virus ppv-s. | five transgenic clones of prunus domestica l. containing plum pox virus (ppv) coat protein (cp) gene and one non-transformed control clone were challenged with ppv-s in the field. symptoms developed on c2, c3, c4, c6 and b70146 but not c5 trees inoculated by chip budding (cbi) (2/2, 2/2, 1/1, 2/2 and 2/2, positive/inoculated) in the first summer after inoculation. however, in the second year, symptoms appeared on cbi c5 trees. the presence of the virus in the plants was confirmed by enzyme-linke ... | 1998 | 10073228 |
| mineral nutrition of plum trees as an important factor of protection against plum pox virus disease. | the experimental results obtained from a fruit garden at krajné pointed out a different content of macrobiogenic elements in the leaves of plum trees infected with plum pox virus (ppv) as compared to healthy ones. mineral nutrition of diseased trees was characteristic by lower relational values of topical levels of macrobiogenic elements especially those of n/p, n/k, (ca + mg)/k, and (n/p)/(n/k). | 1998 | 10073227 |
| high resistance and control of biological risks in transgenic plants expressing modified plum pox virus coat protein. | transgenic plums transformed with the plum pox virus coat protein (ppv cp) gene displayed a resistance to the sharka disease (ravelonandro et al., 1997). however, the expression of ppv cp in transgenic plants may lead to complementation of deficient characteristic of an incoming potyvirus. indeed, an aphid-intransmissible strain of zucchini yellow mosaic virus (zymv-nat) could be transmitted when encapsidated by the engineered ppv cp (lecoq et al., 1993). to control such a risk, new ppv cp const ... | 1998 | 10073226 |
| aphid species--vectors of plum pox virus. | plum pox virus (ppv) is widely spread by natural vectors present in plum orchards. the efficiency of transmission is dependent on the frequency of the occurrence of vectors and the cultivar susceptibility to this pathogen. having in view that ppv has a wide range of annual and multiannual host plants and vectors, there is great concern for obtaining ppv-resistant cultivars. this report deals with the following vectors: hyalopterus pruni, brachycaudus cardui, brachycaudus helichrysi. myzus persic ... | 1998 | 10073225 |
| breeding of plums and prunes resistant to plum pox virus. | at hohenheim we started a plum breeding programme to get new sharka-resistant cultivars with better fruit quality. in the last years we have recognized that the resistance of all cultivated plums is based on quantitative criteria and therefore relative. for this reason we changed our strategy for the resistance breeding. for the first breeding cycle we used special donors with quantitative resistance. today, the qualitative resistance is more integrated. we intend to combine both types of resist ... | 1998 | 10073224 |
| characterization of plum pox virus isolates from slovakia. | plum pox virus (ppv) isolates from stone-fruit trees (plums, myrobalans, apricots and peaches) from orchards and gardens were characterized. to characterize their biological properties, several ppv isolates were transmitted by chip budding to gf 305 seedlings and mechanically to selected herbaceous test plants. the isolates differed in severity of infection, host range and symptomatology. a subgroup differentiation of 43 isolates from 22 localities of western and middle slovakia was accomplished ... | 1998 | 10073223 |
| investigation on the plum pox virus resistance in different apricot genotypes. | in our three-year investigation, 164 apricot trees of different old german varieties cultivated in the mansfelder land region were tested for the plum pox virus (ppv) resistance by double grafting in greenhouse conditions using an isolate of ppv d strain from our region. we selected 25 genotypes with quantitative resistance and two with immunity. the first results of field trials are comparable with those from greenhouse. with cvs. virosia and brevira, two local quantitatively resistant varietie ... | 1998 | 10073222 |
| comparison of different methods of rna isolation for plum pox virus detection by reverse transcription-polymerase chain reaction. | the diagnosis of plum pox virus (ppv) is still considered one of the most important aspects of the "sharka" problem. in fact, different studies demonstrated an uneven distribution of the virus in infected trees due to a high variability in virus concentration. these aspects complicate the ppv diagnosis. to date, biological, serological and molecular assays have been successively developed in order to obtain sensitive and efficient ppv detection techniques. in particular, the polymerase chain rea ... | 1998 | 10073221 |
| relationship between concentration of plum pox virus and content of pigments in virus-infected symptomatic apricot leaves. | besides other factors, occurrence of plum pox virus (ppv)-caused spots and mosaic symptoms on leaves of stone fruits are known to influence important physiological functions including production of assimilates. apricot (prunus armeniaca l.) seedlings were used as a test material for typical manifestation of symptoms of the disease on the foliage. the relations of the abovementioned visual symptoms to the virus and pigments concentrations in leaves have so far not been known sufficiently. we dete ... | 1998 | 10073220 |
| some results of 50 years of research on the resistance to plum pox virus. | over 300 references on the resistance of stone fruit species to plum pox virus (ppv) have been utilized for the summarization of relevant information in this review. methods of testing ppv resistance, procedures of evaluation and characterization of ppv resistance as well as breeding of ppv-resistant cultivars are briefly discussed. altogether 370 cultivars, hybrids and clones of plum, peach, apricot, nectarine and wild prunus species are tabulated together with the authors who have reported on ... | 1998 | 10073219 |
| new device and method for capture, reverse transcription and nested pcr in a single closed-tube. | a device and improved method based on the use of a compartmentalized eppendorf tube that allows capture, reverse transcription and nested-pcr in a single closed-tube has been developed and patented. the main advantages of the system are the high sensitivity obtained, the simplicity, the low risk of contamination and the easy establishment of adequate conditions for nested-pcr. the method has been successfully applied to the detection and characterization of citrus tristeza closterovirus and plum ... | 1999 | 10037824 |
| pest status of american plum borer (lepidoptera: pyralidae) and fruit tree borer control with synthetic insecticides and entomopathogenic nematodes in new york state. | surveys were conducted in 1994 and 1995 to determine the pest status of the american plum borer, euzophera semifuneralis (walker), in new york state stone fruit crops. these surveys indicate that american plum borer is the most important of the wood-boring insects infesting tart cherries and also is an important pest in peaches suffering from canker diseases. it is not prevalent in plums or in healthy peaches. trials to control american plum borer were conducted in tart cherry and peach by using ... | 1999 | 10036985 |
| anoxic and hypoxic immature rat model for measurement of monoamine using in vivo microdialysis. | the immature brain is considered relatively resistant to anoxia and ischemia. although hypoxia without ischemia has not been considered to produce brain damage in immature rats as well as in adult rats (s. levine, anoxic-ischemic encephalopathy in rats, am. j. pathol., 36 (1960) 1-17 [8]; d.e. levy, j.b. brieley, d.g. silverman, f. plum, brief hypoxia-ischemia initially damages cerebral neurons, arch. neurol., 32 (1975) 450-456 [9]; j.e. rice, r.c. vannucci, j.b., brieriey, the influence of imma ... | 1999 | 9974139 |
| mitotic stability of infection-induced resistance to plum pox potyvirus associated with transgene silencing and dna methylation. | plum pox potyvirus (ppv) infection of transgenic nicotiana benthamiana plants that expressed the ppv nib rna replicase carrying a gly to val mutation at the gdd motif (nibv lines) induced a phenotype of virus resistance and transgene silencing, which was not transmissible to the progeny after self-fertilization (h. s. guo and j. a. garcía, mol. plant-microbe interact. 10:160-170, 1997). here, we demonstrate that the induced resistance of nibv plants is mitotically stable after plant propagation ... | 1999 | 9926412 |
| isolation of hiv-1 rna from plasma: evaluation of seven different methods for extraction (part two). | some new commercial methods for the extraction of viral rna have been introduced recently. in addition to the study published previously (verhofstede, c., reniers, s., van wanzeele. f., plum j., 1996. aids 8, 1421-1427), seven different methods (four newly developed and three reference methods) for extraction of hiv-1 rna from plasma have been evaluated. the rna preparation method that gave the best results (acceptable reproducibility, highest sensitivity, reasonable price, fast and easy to perf ... | 1998 | 9923750 |
| susceptibility to recombination rearrangements of a chimeric plum pox potyvirus genome after insertion of a foreign gene. | infectious rna transcripts were generated from a chimeric cdna clone of the plum pox potyvirus (ppv) genome containing the bacterial beta-glucuronidase (gus) gene inserted between the sequences coding for the p1 and hc proteins. an artificial cleavage site specific for the nia viral proteinase was engineered between the gus and hc sequences to produce free gus and hc proteins. the resulting virus ppvgus/ was stably maintained during the first round of infection, although plants remained symptoml ... | 1998 | 9870586 |
| nucleotide sequence of the putative replicase gene of the sour cherry strain of plum pox potyvirus. | the complete nucleotide sequence of the nib coding region of the sour cherry strain of plum pox potyvirus (ppv-soc) has been determined. it consists of 1554 nucleotides and encodes a putative replicase protein of 518 amino acids. sequence identity scores between nib of ppv-soc and other isolates of ppv are significantly low (c. 78%). many of the nucleotide substitutions, however, are silent. ppv-soc differs from isolates of ppv-d, ppv-m and ppv-e1 amar at multiple amino acid positions that are c ... | 1998 | 9856106 |
| ultrastructural localization of nonstructural and coat proteins of 19 potyviruses using antisera to bacterially expressed proteins of plum pox potyvirus. | antisera to the bacterially expressed nonstructural proteins (nsp) hc-pro, ci, nia, and nib and the coat protein (cp) of plum pox potyvirus (ppv) were used for analysing the composition of virus-induced cytoplasmic and nuclear inclusions by electron microscopy. the antisera reacted with nsp and cp of ppv on immunogold-labelled ultrathin sections. antiserum to cp reacted with virions of seven out of 18 other potyviruses. cp was distributed throughout the cytoplasm of infected cells. antisera to p ... | 1998 | 9856098 |
| properties of a virus causing mosaic and leaf curl disease of celosia argentea l. in nigeria. | a sap transmissible virus, causing mosaic and leaf curl disease of celosia argentea, was isolated at vegetable farms in amuwo odofin, tejuoso, and abule ado, lagos, nigeria. the virus had a restricted host range confined to a few species of the amaranthaceae, chenopodiaceae and solanaceae families. it failed to infect several other species of the aizoaceae, brassicaceae, cucurbitaceae, fabaceae, lamiaceae, malvaceae, poaceae and tiliaceae families. the virus was transmitted in a non-persistent m ... | 1998 | 9842442 |
| plum stones: an unusual cause of ileostomy obstruction demonstrated by sonography. | bowel obstruction is a common cause of stomal complications of ileostomy. we report a case in which sonography revealed 2 well-defined, rounded, hyperechoic structures, measuring 1.7 cm in diameter and causing dense acoustic shadowing, in the lumen of the bowel of a 3-year-old boy with a permanent end ileostomy. endoscopy showed the structures to be freely mobile plum stones in the fluid-filled terminal ileum. this case demonstrates the usefulness of sonography in the diagnostic workup of patien ... | 1998 | 9783251 |
| strain variability of plum pox virus isolates from western slovakia. | leaf tissues of stone fruit trees (plum, apricot, peach and myrobalan) carrying symptoms of plum pox virus (ppv) infection and of peach gf 305 seedlings and nicotiana benthamiana infected experimentally with ppv were assayed for ppv by polymerase chain reaction (pcr). the expected 243 bp pcr products were subjected to restriction fragment length polymorphism (rflp) analysis with restriction endonucleases alui and rsai. all of the pcr products contained the alui site. the rsai restriction profile ... | 1998 | 9770072 |
| multigeneration exposure test of drosophila melanogaster to elf magnetic fields. | mutations, other than dominant lethals, were accumulated on wild type second chromosomes (+) of drosophila melanogaster during exposure to 50 hz sinusoidal alternating magnetic fields of 0.5 or 5 mt (rms) for 40 generations by the curly/plum(cy/pm) accumulation method. we maintained, for 40 generations under continuous exposure, each (+) chromosome as a heterozygote with (cy) chromosome. viability of the (+) chromosome was tested by sib-mating of (cy/+) male and (cy/+) female in a culture every ... | 1998 | 9738524 |
| mercury in water and sediments of the southern baltic sea. | total mercury concentrations [hgt] were measured in samples of water and sediments collected from the southern baltic sea from the period 1993-1995. picomolar concentrations of hgt were found in offshore surface water. higher levels were measured in samples from the plum of the vistula. an exceptionally high concentration of mercury (25 pm hgt, 5 pm methylmercury) was measured in the near bottom, quasi-anoxic water of the gdañsk deep and explained by the release of mercury from fine grained sedi ... | 1998 | 9652127 |
| frontiers of neurology: a symposium in honor of fred plum. new york city, new york, usa. october 5, 1996. | 1997 | 9634449 | |
| use of modified plum pox virus coat protein genes developed to limit heteroencapsidation-associated risks in transgenic plants. | aphid transmission of a non-aphid-transmissible strain of zucchini yellow mosaic virus (zymv-nat) occurs in transgenic plants expressing the plum pox potyvirus (ppv) coat protein (cp) gene. heteroencapsidation has been shown to be responsible for this modification in the epidemiological characteristics of the infecting virus. in order to prevent this biological risk, several modified ppv cp constructs were produced that were designed to interfere with heteroencapsidation itself or to block aphid ... | 1998 | 9634095 |
| nicotiana benthamiana plants transformed with the plum pox virus helicase gene are resistant to virus infection. | nicotiana benthamiana domin. plants were transformed with the cytoplasmic inclusion protein (ci) gene of plum pox potyvirus (ppv) to investigate, whether this non-structural protein would be able to confer resistance. the ci protein is an rna helicase, which contains a conserved nucleotide binding motif (ntbm) and plays an important role in viral replication. two gene constructions were developed for plant transformation. the first contains the original coding sequence of the ci gene under the c ... | 1998 | 9617773 |
| development of an antigen presentation system based on plum pox potyvirus. | the development of an antigen presentation system based on the plum pox potyvirus (ppv) is here described. the amino-terminal part of ppv capsid protein was chosen as the site for expression of foreign antigenic peptides. modifications in this site were engineered to avoid the capability of natural transmission by aphids of this ppv vector. as a first practical attempt, different forms of an antigenic peptide (single and tandem repetition) from the vp2 capsid protein of canine parvovirus (cpv) w ... | 1998 | 9607317 |
| susceptibility of peach gf 305 seedlings and selected herbaceous plants to plum pox virus isolates from western slovakia. | the susceptibility of peach gf 305 seedlings and herbaceous plants to five plum pox virus (ppv) isolates from orchards of western slovakia was investigated. ppv was isolated from diseased plum, apricot and peach trees, and transmitted by chip-budding to peach gf 305. the herbaceous plants were infected by mechanical inoculation. the transmission was analysed by symptomatology and double sandwich enzyme-linked immunosorbent assay (das-elisa). infected peaches developed leaf distortion, tissue cle ... | 1997 | 9607094 |
| effect of gamma irradiation on the production of cell wall degrading enzymes by aspergillus niger. | fungi occurring in egyptian fruits in the city of qena were studied. results from the examination of 25 replicated samples of plums, pears and apples are reported. examinations were carried out by direct plating after surface disinfection in a 0.5% (w/v) calcium hypochlorite solution on czapek's-dox agar. the dominant fungus found in the three types of fruit was aspergillus niger, which was present in 88% of plum samples, 80% of pear samples and all of the apple samples. the lowest dose of gamma ... | 1998 | 9600619 |
| specific oligonucleotide primers for the direct detection of plum pox potyvirus-cherry subgroup. | a specific polymerase chain reaction assay was developed for direct identification of the distinct subgroup of plum pox potyvirus (ppv) isolates from cherry trees (ppv-cherry, ppv-c) and its differentiation from other known subgroups of ppv. the specificity of the assay is based on using a pair of primers whose nucleotide sequences are located on the coat protein gene of ppv-sour cherry (soc) at regions of high nucleotide divergence between ppv-soc and other isolates of ppv. the technique will b ... | 1998 | 9562418 |
| a sex-linked ace gene, not linked to insensitive acetylcholinesterase-mediated insecticide resistance in culex pipiens. | an acetylcholinesterase (ache) gene, ace.x, showing 93% identity of deduced amino acid sequence to anopheles stephensi ace has been cloned from a culex pipiens strain homozygous for insensitive ache (iache) mediated insecticide resistance. dna sequence of genomic dna clones identified exons 2-5. rflp of six clones indicated four possible alleles. linkage analysis located ace.x to chromosome i, less than 0.8 centimorgans from the sex locus, whereas the locus conferring resistance was 2.0 centimor ... | 1998 | 9535157 |
| production of strain specific antibodies against a synthetic polypeptide corresponding to the n-terminal region of the plum pox potyvirus coat protein. | comparison of the predicted coat protein amino acid sequence of the 'sweet cherry' strain of plum pox potyvirus (ppv-swc) with the corresponding regions of several other ppv strains indicated that the main differences are in the n-terminal region. polyclonal antibodies were produced against a synthetic peptide corresponding to the 1-14 sequence of the n-terminal region of ppv-swc coat protein. they specifically detected ppv-swc in different immunochemical tests. | 1997 | 9504763 |
| plum pox potyvirus resistance associated to transgene silencing that can be stabilized after different number of plant generations. | nicotiana benthamiana plants were transformed with a fragment of the plum pox potyvirus (ppv) genome that encodes the nuclear inclusion a (nia) and b (nib) proteins and the n-terminus of the capsid protein (nia-nib-cp). lines transformed with this ppv genomic fragment harboring mutations in the gdd replicase-motif were also obtained. plants of niadeltav lines that carry a gdd to vdd mutation in the ppv transgene, were immune to ppv infection. the resistance was highly specific, since it was only ... | 1998 | 9469941 |
| hop stunt viroid (hsvd) sequence variants from prunus species: evidence for recombination between hsvd isolates. | hop stunt viroid (hsvd) is able to infect a number of herbaceous and woody hosts, such as grapevine, citrus or prunus plants. previous phylogenetic analyses have suggested the existence of three major groups of hsvd isolates (plum-type, hop-type and citrus-type). the fact that these groups often contain isolates from only a limited number of isolation hosts prompted the suggestion that group-discriminating sequence variations could, in fact, represent host-specific sequence determinants which ma ... | 1997 | 9400968 |
| simultaneous detection and typing of plum pox potyvirus (ppv) isolates by heminested-pcr and pcr-elisa. | two techniques for simultaneous detection and typing of plum pox potyvirus (ppv) isolates belonging to the d or m serotypes, heminested pcr (h-pcr) and pcr-elisa, have been developed. ten ppv isolates typed using ppv-d and ppv-m specific monoclonal antibodies by elisa-dasi were used to validate these two methods. the results obtained show a complete coincidence of the nucleic acid-based techniques with the serological data. when serial dilutions of infected plant extracts were assayed, h-pcr and ... | 1997 | 9389402 |
| the motif v of plum pox potyvirus ci rna helicase is involved in ntp hydrolysis and is essential for virus rna replication. | the plum pox potyvirus (ppv) protein ci is an rna helicase whose function in the viral life cycle is still unknown. the ci protein contains seven conserved sequence motifs typical of rna helicases of the superfamily sf2. we have introduced several individual point mutations into the region coding for motif v of the ppv ci protein and expressed these proteins in escherichia coli as maltose binding protein fusions. mutations that abolished rna helicase activity also disturbed ntp hydrolysis. no mu ... | 1997 | 9358154 |
| cloning, sequencing, and expression of the mig gene of mycobacterium avium, which codes for a secreted macrophage-induced protein. | mycobacterium avium is an intracellular pathogen that has evolved to be a frequent cause of disseminated infection in immunocompromised patients. although these bacilli are readily phagocytized, they are able to survive and even multiply within human macrophages. the process whereby mycobacteria circumvent the lytic functions of the macrophages is currently not well understood, but this is a key aspect in the pathogenicity of all pathogenic mycobacteria. previously, we identified a gene in m. av ... | 1997 | 9353032 |
| cap-independent leaky scanning as the mechanism of translation initiation of a plant viral genomic rna. | the genome of plum pox virus contains a single open reading frame that is translated into a large polyprotein. although the open reading frame starts at nucleotide 36 (36aug), it is translated from the second, 147aug, which is in a more favourable context for translation initiation. we have carried out in vitro translation and transient expression analysis in protoplasts of a nested set of substitution and deletion mutants, and the results show that no internal structure in the 5' noncoding regi ... | 1997 | 9349492 |
| specific detection of d- and m-isolates of plum pox virus by immunoenzymatic determination of pcr products. | molecular techniques based on the polymerase chain reaction (pcr) can provide rapid and sensitive diagnosis of plum pox virus (ppv), the causal agent of the devastating 'sharka' disease of stone fruit trees. the present study compared routine polymerase chain reaction (pcr) procedures against a new system, pcr-elisa (boehringer mannheim), which enables immunoenzymatic detection of pcr products. the results show that this hybridisation system ensures fast and more sensitive detection of ppv assoc ... | 1997 | 9300377 |
| design of a solubilization pathway for recombinant polypeptides in vivo through processing of a bi-protein with a viral protease. | an artificial maturation pathway for increasing the solubility in vivo of recombinant proteins overproduced in escherichia coli is reported, which is based on the proteolytic processing of viral polyproteins. the gene product of interest is expressed as a fusion to a heterologous moiety (i.e. the maltose binding protein, male) in order to increase the overall solubility of the hybrid. the hinge region between the two fusion partners contains a cleavage site for the nia protein, a very specific p ... | 1997 | 9278287 |
| a case-control study of risk factors of nasopharyngeal carcinoma. | risk factors and nasopharyngeal carcinoma (npc) in residents of guangzhou city were investigated in a case-control study: 104 cases were compared with an equal number of age, sex, and neighborhood-matched controls. the results of multiple conditional regression of logistic model showed that plum vegetable (adjusted or = 1.81, 95% ci 1.01-3.33), preserved prune (adjusted or = 2.95, 95% ci 1.04-8.41), no separate kitchen (if >35 years, adjusted or = 1.98, 95% ci 1.24-3.75), kitchen range without c ... | 1997 | 9233826 |
| serological characterization of hungarian plum pox virus isolates. | three hungarian (no. 2, 4 and 9), and a moldavian (k) plum pox virus isolates were compared with a characterized spanish isolate (5.15) by rt-pcr, elisa, dot-blot and western blot analysis. monoclonal antibodies prepared against the external, intermediate and internal sequences of the coat protein of the spanish isolate were able to differentiate the four isolates. hungarian isolate no. 2 proved to be serologically identical to the spanish isolate, while no. 4 showed appreciable differences and ... | 1997 | 9232895 |
| fungi associated with post-harvest rot of black plum (vitex doniana) in nigeria. | the fungi associated with rot of vitex doniana fruits (blackplum) were isolated and identified. aspergillus niger, botryodiplodia theobromae, candida spp. penicillium chrysogenum, rhizopus stolonifer, fusarium pallidoroseum f. oxysporum and mucor mucedo were the primary rot causing fungi in contrast to cladosporium herbarum and mucor circinelloides which were just present as secondary colonizers. the rot fungi penetrated mainly through wounds and bruises on the surface of fruits. mature green fr ... | 1996 | 9208478 |
| long sequences in the 5' noncoding region of plum pox virus are not necessary for viral infectivity but contribute to viral competitiveness and pathogenesis. | the 5'-terminal 31 nucleotides of the 146-nucleotides-long 5' noncoding region of plum pox potyvirus (ppv) are highly conserved in all the members of the potyvirus genus. to map the sequences of the 5' noncoding region that are necessary in vivo for infectivity, we have constructed a nested set of substitution and deletion mutants. while we were not able to infect nicotiana clevelandii plants with full-length ppv transcripts bearing mutations in the 5'-terminal 35 nucleotides of the viral genome ... | 1997 | 9201225 |
| analysis of the sequence diversity of the p1, hc, p3, nib and cp genomic regions of several yam mosaic potyvirus isolates: implications for the intraspecies molecular diversity of potyviruses. | partial sequences from serologically characterized yam mosaic potyvirus (ymv) isolates were determined in conserved (helper-component proteinase, hc; nuclear inclusion b, nib) and variable (first protein, p1; third protein, p3; and coat protein, cp) regions of the potyviral genome in order to investigate the intraspecies molecular diversity of ymv. multiple sequence alignments and pairwise comparisons were used to quantify the sequence polymorphism in these regions. two levels of diversity were ... | 1997 | 9191916 |
| processing of the plum pox virus polyprotein at the p3-6k1 junction is not required for virus viability. | proteolytic processing of the potyvirus polyprotein is mainly performed by the virus-encoded nia protease, whose cleavage sites are characterized by conserved heptapeptide sequences. partial processing at the cleavage site present between the p3 and 6k1 cistrons by the plum pox potyvirus (ppv) nia protease has been previously shown to occur in vitro. we have now studied the role of polyprotein processing at the p3-6k1 junction in vivo, using a full-length ppv cdna clone. ppv mutant transcripts c ... | 1995 | 9049341 |
| agenesis of the corpus callosum associated with paroxysmal hypothermia: shapiro's syndrome. | spontaneous recurrent hypothermia and hyperhidrosis associated with agenesis of the corpus callosum was first described by shapiro and plum in 1967. since then, several cases with similar symptoms (now known as shapiro's syndrome or spontaneous periodic hypothermia) have been described. we report another case of this syndrome in a 21-year-old-man, and discuss possible pathogenetic mechanisms and therapeutic approaches. | 1997 | 9038041 |
| physico-chemical and sensory properties of formulated syrup from black plum (vitex doniana) fruit. | the fresh ripe fruit pulp and syrup, produced using the open pan evaporator, of vitex doniana were analysed physico-chemically. results show that the edible pulp of the ripe fruit is fairly rich in vitamin c (18.1 mg/100 ml) but acidic (ph 4.38) and poor in protein (0.82%) and oil (0.56%). the syrup yielded a de value of 52, total dissolved solids content of 67 degrees brix and a refractive index of 1.4762 at 20 degrees c. the syrup was moderately viscous (2.1 poiseuille), acid (ph 4.45) but rel ... | 1996 | 8983056 |
| a nurse with anaphylaxis to stone fruits and latex sensitivity: potential diagnostic difficulties to consider. | there have been several recent reports describing a cross-reactivity between latex and antigens derived from a number of fruits, including those of the stone fruit family. the relationships between the allergic reactions to the antigens from these plant products are still being defined. | 1996 | 8970444 |
| epidemiologic association between antibody titres against bovine virus diarrhoea virus, rinderpest disease virus and infectious bovine rhinotracheitis virus in a buffalo herd. | the association between antibody titres against bovine virus diarrhoea virus (bvdv), infectious bovine rhinotracheitis virus (ibrv) and rinderpest disease virus (rpdv) in buffaloes was investigated in a cross-sectional study. thirty-six lactating buffaloes from a herd in landhi cattle colony, karachi were bled and serum samples subjected to a micro-neutralisation test at the plum island animal disease center, usa, to categorise each buffalo either as positive or negative against each of 3 viral ... | 1996 | 8888525 |
| first findings of plum pox virus in walnut trees (juglans regia l.) | plum pox virus (ppv) was transmitted from infected buds and leaves of walnut tree (juglans regia l.) on the following herbaceous indicators: chenopodium foetidum schrad., nicotiana bigelovii var. quadrivalvis fuchs., n. clevelandii x n. glutinosa. a positive elisa reaction with antisera against ppv was obtained from infected buds and leaves of juglans regia l. and from attacked leaves of indicator plants. | 1996 | 8886101 |
| unusual foreign bodies of upper gastrointestinal tract. | we report management of unusual foreign bodies of upper gastrointestinal tract, namely beer bottle cap, raisins and pistachu, mango peel, betelnut and plum seed at a university hospital in northern india. | 1995 | 8854958 |
| frequent occurrence of recombinant potyvirus isolates. | we have performed a systematic search for recombination in the region encoding coat protein and the 3' non-translated region in natural isolates of potyviruses, the largest group of plant rna viruses. the presence of recombination, and the localization of the cross-over points, were confirmed statistically, by three different methods. recombination was detected or suspected in 18 out of 109 potyvirus isolates tested, belonging to four out of eight virus species, and was most prevalent in potato ... | 1996 | 8760448 |
| cold calcium signaling in arabidopsis involves two cellular pools and a change in calcium signature after acclimation. | cold shock elicits an immediate rise in cytosolic free calcium concentration ([ca2+]cyt) in both chilling-resistant arabidopsis and chilling-sensitive tobacco (nicotiana plumbaginifolia). in arabidopsis, lanthanum or egta caused a partial inhibition of both cold shock [ca2+]cyt elevation and cold-dependent kin1 gene expression. this suggested that calcium influx plays a major role in the cold shock [ca2+]cyt response and that an intracellular calcium source also might be involved. to investigate ... | 1996 | 8721751 |
| [polyostotic hyperostosis in a plum-headed parakeet (psittacula cyanocephala l., 1766]. | with emphasis on a case report, the clinical and pathological findings commonly associated with polyostotic hyperostosis as well as its etiology are discussed, and the available literature is reviewed. the diagnostic specifics of the disease are detailed, and a survey of possible differential diagnoses is given. | 1996 | 8720953 |
| the rna helicase ci from plum pox potyvirus has two regions involved in binding to rna. | the plum pox virus (ppv) protein ci is an rna helicase, whose function in the virus replication is still unknown. recently, an rna binding domain was mapped to a region of the ci protein that includes the arginine-rich motif vi typical of rna helicases of the superfamily sf2. in the present study, a second region involved in rna binding activity of the ci protein has been identified. northwestern assays with a series of maltose-binding protein fusions that contain different ci fragments showed t ... | 1996 | 8690088 |
| a modified device for long term sampling of parotid saliva in various experimental conditions. | a parotid cup has been widely used as an intraoral device for the collection of parotid saliva. however, in long duration sampling, the cup tends to disconnect under circumstances such as mastication, sleep or physical exercise. we have designed a modified device of parotid cup for saliva collection to overcome these unfavorable conditions. the parotid cup was modified by fixing it to the bite-plate appliance which was adjusted individually to the occlusal plane in order to avoid disconnection o ... | 1995 | 8689745 |
| print-capture pcr: a simple and highly sensitive method for the detection of plum pox virus (ppv) in plant tissues. | 1996 | 8668554 | |
| effects of land use on the degradability of dissolved organic matter in three watersheds of the plum island sound estuary. | 1995 | 8541418 | |
| localization of fruit tree viruses by immuno-tissue printing in infected shoots of malus sp. and prunus sp. | immuno-tissue printing protocols for the localization of apple chlorotic leaf spot virus (aclsv), stem grooving virus (sgv) and plum pox virus (ppv) in shoots of prunus and malus in vitro have been established for routine diagnosis in a virus elimination program. since these viruses belong to different virus genera, the protocols were adapted according to the properties of the virus under investigation. accumulation of aclsv was highest in the base of the stem and decreased towards the apex of t ... | 1995 | 8537455 |
| berenil binding to higher ordered nucleic acid structures: complexation with a dna and rna triple helix. | berenil is an antitrypanosomal agent that binds to nucleic acid duplexes. recently, we reported that this drug can bind to both dna and rna duplexes, while exhibiting properties characteristic of both intercalation and groove binding [pilch, d. s., kirolos, m. a., liu, x., plum, g. e., & breslauer, k. j. (1995) biochemistry 34, 9962-9976]. in this work, we use spectroscopic, calorimetric, and hydrodynamic techniques to demonstrate that berenil also can bind to dna and rna triplexes. our results ... | 1995 | 8519768 |
| immunodetection of the plum pox virus helper component in infected plants and expression of its gene in transgenic plants. | tobacco plants (nicotiana tabacum cv. xanthi) have been transformed via agrobacterium tumefaciens vectors, with cdnas corresponding to the plum pox virus (ppv) cistron 2 encoding helper component (hc-pro) and with the first two and half cistrons of the ppv genome. presence of the hc-pro in ppv-infected plants and transgenic plants transformed with the gene coding for this protein was investigated using specific polyclonal antibodies produced against the ppv hc-pro. the results suggest that two p ... | 1993 | 8517789 |
| 3'-terminal sequence of the plum pox virus ps and ŏ6 isolates: evidence for rna recombination within the potyvirus group. | the sequence of the 3'-terminal 1768 nucleotides of the ps and ŏ6 isolates of plum pox virus (ppv) has been determined and compared with that of the equivalent regions of other ppv isolates sequenced previously. the sequenced region is part of the ppv open reading frame encoding the last 186 amino acids of the nib protein and the coat protein (cp, 330 amino acids), followed by a non-coding region of 220 nucleotides and a poly(a) tail. ppv-ps and ppv(-)ŏ6, just like ppv-el amar, show rather high ... | 1993 | 8445362 |
| complete nucleotide sequence of the genome of an apple isolate of apple chlorotic leaf spot virus. | the complete nucleotide sequence of the genome of an apple isolate of apple chlorotic leaf spot virus (aclsva) was determined. the genome is 7552 nucleotides excluding the poly(a) tail and contains three open reading frames (orfs 1, 2 and 3), encoding proteins with m(r) values of 216,503 (216.5k), 50,453 (50.4k) and 21,394 (21.4k), respectively. nucleotide sequence comparisons between aclsv-a and the previously sequenced aclsv from plum (aclsv-p) showed that the sequence identity at the nucleoti ... | 1993 | 8376968 |
| inhibitory effects of human cystatin c on plum pox potyvirus proteases. | the effect of different protease inhibitors on the proteolytic processing of the plum pox potyvirus (ppv) polyprotein has been analyzed. human cystatin c, an inhibitor of cysteine proteases, interfered with the autoprocessing of the viral papain-like cysteine protease hcpro. unexpectedly, it also had an inhibitory effect on the autocatalytic cleavage of the nla protease which, although it has a cys residue in its active center, has been described as structurally related to serine proteases. othe ... | 1993 | 8343605 |
| systemic phaeohyphomycosis caused by exophiala dermatitidis. | we report a case of systemic phaeohyphomycosis due to exophiala dermatitidis in a 24-year-old man. at the age of 17, the patient had noticed cervical swellings. on palpation at his initial examination, more than a dozen firm lymph nodes between 1 and 5 cm in diameter were found on both sides of the neck and in both axillae. examination of biopsy specimens of lymph nodes revealed pale brown hyphae in granulomatous lesions and exophiala dermatitidis was isolated from mycological cultures of the lo ... | 1993 | 8316255 |
| monoclonal antibodies suitable for plum pox virus determination. | monoclonal antibodies (mabs) to plum pox virus (ppv) were obtained after immunization of balb/c mice with purified ppv-w isolate. spleen cells from a mouse showing a high serum titer were used for fusion with sp2/0-ag14 myeloma cells. culture supernatants were screened for specific antibody production against ppv-w isolate using indirect elisa. a total of six stable hybridoma lines producing mabs of igg class were obtained. all four ppv isolates tested (w, a, d and m) can be distinguished by the ... | 1993 | 8314598 |
| care allegedly provided without proper supervision. case in point: raicevich v. plum creek medical p.c. 918 f. supp. 2d 929--co (1993). | 1993 | 8265797 | |
| treatment of facial spasm by plum-blossom needle tapping and cupping therapy. | 1993 | 8246597 | |
| survey of mycoflora and mycotoxins of some dried fruits in egypt. | fifty-five species and two varieties appertaing to 23 genera were collected and identified from 4 samples of dried fig and 3 dried samples of each of apricot, plum and raisin. forty-nine species and two varieties belonging to 20 genera were isolated on 1% glucose-czapek's while 31 species and one variety belonging to 16 genera were isolated on 40% sucrose-czapek's agar medium at 28 +/- 2 degrees c. penicillium, aspergillus and cladosporium were the common genera on the two types of media used. a ... | 1993 | 8229671 |
| comparative sequence analysis of four complete primary structures of plum pox virus strains. | the complete nucleotide sequence of plum pox virus (ppv) strain sk 68 was determined from a series of overlapping cdna clones. the exact 5' terminus was determined by direct rna sequencing. the rna sequence was 9786 nucleotides in length, excluding a 3' terminal poly(a) sequence. the large open reading frame starts at nucleotide position 147 and is terminated at position 9568. comparison of cistrons from other plum pox virus strains with those predicted for the sk 68 strain indicated the same ge ... | 1993 | 8122394 |
| effect on lipopolysaccharide structure of aeration during growth of a plum isolate of pseudomonas syringae pv. morsprunorum. | the composition of lipopolysaccharide (lps) extracted with aqueous phenol from a virulent english plum isolate of pseudomonas syringae pv. morsprunorum varied according to the partial pressure of oxygen (po2) in the culture medium at the time of harvest. when po2 was low, the organism grew slowly and produced smooth lps bearing rhamnan sidechains. as po2 was raised, the rate of growth increased and smooth lps was replaced by a rough species deficient in rhamnose, which co-extracted with a d-gluc ... | 1994 | 8075800 |
| parkinson and the plum tree. | 1994 | 7986170 | |
| fibre constituents of some foods. | some plant foods viz. bottlegourd, carrot, cauliflower, cabbage, green bengalgram, pea, apple, plum, guava, karonda, blackgram husk and lentil husk were analysed for their dietary fibre components. the total dietary fibre contents of these foods varied from 14.68 to 78.21 percent on dry matter basis. as compared to fruits and vegetables, the husks had higher amount of total dietary fibre. cellulose represented as the major fibre constituent in most of the foods whereas, husks were observed to be ... | 1994 | 7971775 |
| nectar feeding by the early-spring mosquito aedes provocans. | nectar feeding by males and females of the mosquito aedes provocans was studied at a site near belleville, ontario, canada. canada plum, prunus nigra, and especially pin cherry, p. pensylvanica, bloomed contemporaneously with the emergence of ae. provocans and were important nectar sources for adult mosquitoes during their first week of life. blossoms of p. pensylvanica shielded for 24 h from foragers produced an average of 0.14 mg of sugar (approximately 2.3j). this nectar was avidly sought by ... | 1994 | 7949310 |
| allergenic cross-reactivity among peach, apricot, plum, and cherry in patients with oral allergy syndrome: an in vivo and in vitro study. | oral allergy syndrome in response to fruits and vegetables frequently occurs as clusters of hypersensitivity to members of the same botanical family, for which the immunologic basis lies in a number of common allergens, most of them still unidentified. | 1994 | 7930303 |
| nucleotide sequence of the coat protein gene of the skierniewice isolate of plum pox virus (ppv). | the coat protein (cp) gene of the skierniewice isolate of plum pox virus (ppv-s) has been amplified using the reverse transcription--polymerase chain reaction (rt-pcr), cloned and sequenced. the nucleotide sequence of the gene and the deduced amino-acid sequence of ppv-s cp were compared with those of other ppv strains. the nucleotide sequence showed very high homology to most of the published sequences. the motif: asp-ala-gly (dag), important for the aphid transmissibility, was present in the a ... | 1994 | 7913278 |
| simple and rapid preparation of infected plant tissue extracts for pcr amplification of virus, viroid, and mlo nucleic acids. | a rapid, simple method for preparing plant tissues infected with viruses, viroids, or mlos using a commercial product known as gene releaser is described. the gene releaser polymeric matrix method produced plant extracts suitable for pcr amplification without the use of organic solvents, ethanol precipitation, or additional nucleic acid purification techniques. modification of maceration methods and/or extraction buffers resulted in the pcr amplification of potato spindle tuber, apple scar skin, ... | 1994 | 7868647 |
| development and preliminary testing of the neurological assessment instrument. | the purpose of this study was to test a neurological assessment instrument (nai), used to assess neurological function in adults with acute brain lesions. instrument items were designed to reflect different aspects of neurological function and assess consciousness along the arousal/awareness continuum as described by plum and posner. content of the nai was validated by 10 neuroscience expert physicians, nurses and speech pathologists. reliability was tested with one pair of raters on 39 neurolog ... | 1994 | 7829918 |
| [occurrence of aluminum in some foodstuffs]. | results of aluminium determinations in breads and biscuits as well as in fish preserves, vegetables, strawberries and teas including fruit-herb teas are presented. also the percent of extraction of aluminium of various kind of tea leaves into the infusions was determined. the commercially available sample of foods was used for investigation. aluminium was determined by using two methods: a spectrophotometric extraction method with-hydroxyquinoline and less sensitive aas method with nitrous oxide ... | 1994 | 7777774 |
| sensitive non-radioactive nucleic acid hybridization assay for plum pox virus detection. | a new non-radioactive sandwich hybridization assay was designed to simplify the analysis of a large number of plant samples. plant material was homogenized in 0.5% sds and added directly to the hybridization reaction, in which a pair of identifying probes were used. one of the probes was biotinylated capture rna specific for plum pox virus (ppv) strain sk-68; the other rna probe was synthesized from a plasmid bearing the adjacent sequence of this strain and was labelled with digoxigenin (dig). b ... | 1994 | 7709075 |
| [long-term survival following excision of a neck carotid body tumor]. | farmer with a jugular tumor on the right side and suffering from head and shoulder aches, and epileptic seizures during sleep was admitted to a district hospital and underwent surgery. partial anesthesia was applied and neck vascular area exposed. intraoperatively a tumor of plum size was seen which constricted carotid artery bifurcation with its branches. the pulse in the internal carotid artery was impalpable. carotid artery was clamped, and conversation with the patient begun to find out whet ... | 1994 | 7708559 |