Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| deletion of zmp1 improves mycobacterium bovis bcg-mediated protection in a guinea pig model of tuberculosis. | having demonstrated previously that deletion of zinc metalloprotease zmp1 in mycobacterium bovis bcg increased immunogenicity of bcg vaccines, we here investigated the protective efficacy of bcg zmp1 deletion mutants in a guinea pig model of tuberculosis infection. zmp1 deletion mutants of bcg provided enhanced protection by reducing the bacterial load of tubercle bacilli in the lungs of infected guinea pigs. the increased efficacy of bcg due to zmp1 deletion was demonstrated in both bcg pasteur ... | 2015 | 25657094 |
| influence of polymerase brand on microarray-based spoligotyping in low concentrations of mycobacterial dna. | spoligotyping is a widely used typing method for the mycobacterium tuberculosis complex. protocols and platforms can be adapted for direct use on patient samples. serial dilutions of genomic dna from mycobacterium bovis bcg strain dsm45071 were spoligotyped by array hybridization using 32 different commercial pcr polymerase preparations. in samples with very low concentrations of mycobacterial dna, commercially available pcr polymerases differed in their performance, and some yielded no, or fals ... | 2015 | 25656919 |
| development of the new zealand strategy for local eradication of tuberculosis from wildlife and livestock. | we describe the progressive development of new zealand's national strategy for control of tuberculosis (tb) in its agricultural sector over the last four decades. the strategy is globally unique, reflecting the need for effective and co-ordinated management of tb in a wildlife maintenance host, the brushtail possum (trichosurus vulpecula), in addition to controlling infection in cattle and farmed deer herds. since the early 1990s, the strategy has been developed by the animal health board (ahb), ... | 2015 | 25651829 |
| antemortem diagnosis of mycobacterium bovis infection in free-ranging african lions (panthera leo) and implications for transmission. | diagnosis of tuberculosis in wildlife often relies on postmortem samples because of logistical challenges and lack of field-friendly techniques for live animal testing. confirmation of infection through detection of infectious organisms is essential for studying the pathogenesis and epidemiology of disease. we describe the application of a technique to obtain respiratory samples from free-ranging living lions to facilitate detection of viable mycobacterium bovis under field conditions. we identi ... | 2015 | 25647595 |
| the mycobacterium tuberculosis outer membrane channel protein cpnt confers susceptibility to toxic molecules. | mycobacterium tuberculosis, the causative agent of tuberculosis, is protected from toxic solutes by an effective outer membrane permeability barrier. recently, we showed that the outer membrane channel protein cpnt is required for efficient nutrient uptake by m. tuberculosis and mycobacterium bovis bcg. in this study, we found that the cpnt mutant of m. bovis bcg is more resistant than the wild type to a large number of drugs and antibiotics, including rifampin, ethambutol, clarithromycin, tetra ... | 2015 | 25645841 |
| low expression level of glna1 accounts for absence of cell wall associated poly-l-glutamate/glutamine in mycobacterium smegmatis. | cell wall associated poly-l-glutamine (plg) layer synthesis is directly linked to glutamine synthetase (gs) encoded by glna1 in tuberculosis causing mycobacteria. avirulent mycobacterium smegmatis (m. smegmatis) despite of having a glna1 homolog lacks cell wall associated plg layer. in the present study, we complemented a δglna1 mutant of mycobacterium bovis (lack plg in cell wall) with m. smegmatis glna1 cloned under m. bovis glna1 promoter. plg synthesis was restored in the cell wall of comple ... | 2015 | 25637529 |
| evaluation of mycobacterium bovis double knockout mce2-phop as candidate vaccine against bovine tuberculosis. | in this study, a mycobacterium bovis knockout strain in phop-phor and mce2 operons was tested as an antituberculosis experimental vaccine in animal models. the double mutant strain was significantly more attenuated than the wild type strain in inmunocompetent and inmunodeficient mice. vaccination with the double mutant protected mice against challenge with a virulent m. bovis strain. | 2015 | 25620706 |
| disease control in wildlife: evaluating a test and cull programme for bovine tuberculosis in african buffalo. | providing an evidence base for wildlife population management is difficult, due to limited opportunities for experimentation and study replication at the population level. we utilized an opportunity to assess the outcome of a test and cull programme aimed at limiting the spread of mycobacterium bovis in african buffalo. buffalo act as reservoirs of m. bovis, the causative agent of bovine tuberculosis (btb), which can have major economic, ecological and public health impacts through the risk of i ... | 2016 | 25620619 |
| histopathology of disseminated mycobacterium bovis infection complicating intravesical bcg immunotherapy for urothelial carcinoma. | intravesical instillation of bacillus calmette-guérin (bcg) is a mainstay of adjunctive therapy for superficial bladder cancer. disseminated bcg infection ("bcg-osis") after this therapy is rare and potentially life-threatening; only isolated case reports detail the histopathologic findings thereof, few of which had a diagnosis confirmed by molecular testing. we report 3 additional cases of bcg-osis complicating bcg therapy, all confirmed by cultures and molecular assays, including the first cas ... | 2015 | 25614462 |
| social and economic aspects of the transmission of pathogenic bacteria between wildlife and food animals: a thematic analysis of published research knowledge. | wildlife is a known reservoir of pathogenic bacteria, including mycobacterium bovis and brucella spp. transmission of these pathogens between wildlife and food animals can lead to damaging impacts on the agri-food industry and public health. several international case studies have highlighted the complex and cross-sectoral challenges involved in preventing and managing these potential transmission risks. the objective of our study was to develop a better understanding of the socio-economic aspec ... | 2015 | 25611914 |
| mazf ribonucleases promote mycobacterium tuberculosis drug tolerance and virulence in guinea pigs. | toxin-antitoxin (ta) systems are highly conserved in members of the mycobacterium tuberculosis (mtb) complex and have been proposed to play an important role in physiology and virulence. nine of these ta systems belong to the mazef family, encoding the intracellular mazf toxin and its antitoxin, maze. by overexpressing each of the nine putative mazf homologues in mycobacterium bovis bcg, here we show that rv1102c (mazf3), rv1991c (mazf6) and rv2801c (mazf9) induce bacteriostasis. the constructio ... | 2015 | 25608501 |
| neither primary nor memory immunity to mycobacterium tuberculosis infection is compromised in mice with chronic enteric helminth infection. | previously we had reported that nippostrongylus brasiliensis, a helminth with a lung migratory phase, affected host resistance against mycobacterium tuberculosis infection through the induction of alternatively activated (m2) macrophages. several helminth species do not have an obligatory lung migratory phase but establish chronic infections in the host that include potent immune downregulatory effects, in part mediated through induction of a foxp3(+) t regulatory cell (treg) response. treg cell ... | 2015 | 25605766 |
| inactivation of mycobacterium bovis ssp. caprae in high-temperature, short-term pasteurized pilot-plant milk. | experiments to determine the efficacy of high temperature, short time (htst) pasteurization of milk in terms of inactivation of pathogenic microorganisms were mainly performed between 1930 and 1960. among the target organisms were mycobacterium bovis and mycobacterium tuberculosis. as a result, the codex alimentarius prescribes that htst treatment of milk should lead to a significant reduction of pathogenic microorganisms during milk pasteurization. due to the development of improved methods for ... | 2015 | 25597969 |
| wrist tenosynovitis due to mycobacterium bovis infection: case series and review of the literature. | tuberculosis infections are still one of the most important public health problems among developing countries. musculoskeletal involvement represents 10-15% of all extrapulmonary cases. tuberculosis tenosynovitis is usually misdiagnosed as nonspecific tenosynovitis. to avoid misdiagnosis and mistreatment, it is important to be alert for mycobacterial infections. this article presents 3 patients with wrist tenosynovitis, which was caused by mycobacterium bovis infection. the article also includes ... | 2014 | 25587496 |
| managing and eradicating wildlife tuberculosis in new zealand. | tuberculosis (tb) due to mycobacterium bovis infection was first identified in brushtail possums (trichosurus vulpecula) in new zealand in the late 1960s. since the early 1970s, possums in new zealand have been controlled as part of an ongoing strategy to manage the disease in livestock. the tb management authority (tbfree new zealand) currently implements three strategic choices for disease-related possum control: firstly tb eradication in areas selected for eradication of the disease from live ... | 2015 | 25582863 |
| spatiotemporal and ecological patterns of mycobacterium microti infection in wild boar (sus scrofa). | mycobacterium microti has recently been described as the causative agent of tuberculosis-like lesions in wild boar (sus scrofa), a reservoir specie of mycobacterium tuberculosis complex (mtbc) in some european mediterranean ecosystem. through a five-year survey on tuberculosis in free-living wild boars, the epidemiological trend of m. microti infections and the host and population risk factors linked with its occurrence were described. retropharyngeal and mandibular lymph nodes of 3041 hunted wi ... | 2016 | 25580561 |
| recombinant tb9.8 of mycobacterium bovis triggers the production of il-12 p40 and il-6 in raw264.7 macrophages via activation of the p38, erk, and nf-κb signaling pathways. | the tb9.8 of mycobacterium bovis can induce strong antigen-specific t-cell responses in proliferation assays and ifn-γ assays. however, whether and how tb9.8 activates innate immune cells remain unclear. therefore, recombinant protein tb9.8 (rtb9.8)-induced proinflammatory cytokine profile by raw264.7 cells was investigated and the related signaling pathway was studied. stimulation with rtb9.8 triggered raw264.7 cells to produce il-6 and il-12 p40. in addition, rtb9.8 activated the mitogen-activ ... | 2015 | 25577342 |
| effectiveness of routine bcg vaccination on buruli ulcer disease: a case-control study in the democratic republic of congo, ghana and togo. | the only available vaccine that could be potentially beneficial against mycobacterial diseases contains live attenuated bovine tuberculosis bacillus (mycobacterium bovis) also called bacillus calmette-guérin (bcg). even though the bcg vaccine is still widely used, results on its effectiveness in preventing mycobacterial diseases are partially contradictory, especially regarding buruli ulcer disease (bud). the aim of this case-control study is to evaluate the possible protective effect of bcg vac ... | 2015 | 25569674 |
| in-silico metabolome target analysis towards panc-based antimycobacterial agent discovery. | mycobacterium tuberculosis, the main cause of tuberculosis (tb), has still remained a global health crisis especially in developing countries. tuberculosis treatment is a laborious and lengthy process with high risk of noncompliance, cytotoxicity adverse events and drug resistance in patient. recently, there has been an alarming rise of drug resistant in tb. in this regard, it is an unmet need to develop novel antitubercular medicines that target new or more effective biochemical pathways to pre ... | 2015 | 25561926 |
| multiple drug-susceptibility screening in mycobacterium bovis: new nucleotide polymorphisms in the embb gene among ethambutol susceptible strains. | pyrazinamide-resistant mycobacterium bovis isolates of animal origin were assessed for drug susceptibility to five antituberculosis drugs by the agar based middlebrook 7h11 method as gold standard as well as by a simplified, dichotomous resazurin microtitre assay (d-rema). | 2015 | 25554388 |
| transcriptome changes upon in vitro challenge with mycobacterium bovis in monocyte-derived macrophages from bovine tuberculosis-infected and healthy cows. | as innate immune cells, macrophages are expected to respond to mycobacterial infection equally in both mycobacterium bovis-infected cows and healthy cows. we previously found that monocyte-derived macrophages (mdms) from m. bovis-infected cows respond differently than mdms from healthy cows when exposed to in vitro m. bovis challenge. we have now used the agilent™ bovine gene expression microarray to examine transcriptional differences between these mdms. at a high multiplicity of infection (10) ... | 2015 | 25550244 |
| pcr-based genomic deletion analysis of rd-regions in the identification of mycobacteria isolated from adverse events following bcg vaccination or tb suspected cases. | early identification of mycobacterial species is crucial for early diagnosis. pcr-multiplex method performed on randomly chosen 54 mycobacteria isolates originating from clinical samples was found to be an inexpensive, quick and reliable alternative for commercially available diagnostics tests. although the results of gene probes identification performed by ntldr were generally consistent with multiplex pcr, two mixed mycobacterium bovis bcg/mycobacterium tuberculosis infections and a single mis ... | 2014 | 25546948 |
| tuberculosis vaccine types and timings. | traditionally, the design of new vaccines directed against mycobacterium tuberculosis, the most successful bacterial pathogen on the planet, has focused on prophylactic candidates that would be given to individuals while they are still young. it is becoming more apparent, however, that there are several types of vaccine candidates now under development that could be used under various conditions. thus, in addition to prophylactic vaccines, such as recombinant mycobacterium bovis bcg or bcg-boost ... | 2015 | 25540272 |
| mycobacterial rna isolation optimized for non-coding rna: high fidelity isolation of 5s rrna from mycobacterium bovis bcg reveals novel post-transcriptional processing and a complete spectrum of modified ribonucleosides. | a major challenge in the study of mycobacterial rna biology is the lack of a comprehensive rna isolation method that overcomes the unusual cell wall to faithfully yield the full spectrum of non-coding rna (ncrna) species. here, we describe a simple and robust procedure optimized for the isolation of total ncrna, including 5s, 16s and 23s ribosomal rna (rrna) and trna, from mycobacteria, using mycobacterium bovis bcg to illustrate the method. based on a combination of mechanical disruption and li ... | 2015 | 25539917 |
| spatial dynamics of bovine tuberculosis in the autonomous community of madrid, spain (2010-2012). | progress in control of bovine tuberculosis (btb) is often not uniform, usually due to the effect of one or more sometimes unknown epidemiological factors impairing the success of eradication programs. use of spatial analysis can help to identify clusters of persistence of disease, leading to the identification of these factors thus allowing the implementation of targeted control measures, and may provide some insights of disease transmission, particularly when combined with molecular typing tech ... | 2014 | 25536514 |
| comparative study of the growth and survival of recombinant mycobacterium smegmatis expressing mce4a and mce4e from mycobacterium bovis. | cholesterol is important for the growth and persistence of mycobacterium tuberculosis in macrophages. the mce4 locus, which is conserved in both m. tuberculosis and mycobacterium bovis, is thought to be responsible for cholesterol transport into the bacteria. however, the exact roles of specific genes within the sophisticated mce4 system remain poorly understood. in this study, mce4a and mce4e of m. bovis, two proteins that are encoded by the mce4 locus, were expressed in mycobacterium smegmatis ... | 2015 | 25536284 |
| highly purified mycobacterial phosphatidylinositol mannosides drive cell-mediated responses and activate nkt cells in cattle. | mycobacterial lipids play an important role in the modulation of the immune response upon contact with the host. using novel methods, we have isolated highly purified phosphatidylinositol mannoside (pim) molecules (phosphatidylinositol dimannoside [pim2], acylphosphatidylinositol dimannoside [acpim2], diacyl-phosphatidylinositol dimannoside [ac2pim2], acylphosphatidylinositol hexamannoside [acpim6], and diacylphosphatidylinositol hexamannoside [ac2pim6]) from virulent mycobacterium tuberculosis ... | 2015 | 25499010 |
| tuberculosis in swine co-infected with mycobacterium avium subsp. hominissuis and mycobacterium bovis in a cluster from argentina. | summary in argentina little is known about the epidemiology of tuberculosis (tb) infection in swine. we characterized the epidemiological dynamics of mycobacterium avium complex (mac) infection in a swine population of argentina using molecular tools and spatial analysis techniques. isolates (n = 196) obtained from tb-like lesions (n = 200) were characterized by polymerase chain reaction. the isolates were positive to either m. bovis (is6110) (n = 160) or m. avium (is1245) (n = 16) while the rem ... | 2015 | 25496827 |
| prioritisation of wildlife pathogens to be targeted in european surveillance programmes: expert-based risk analysis focus on ruminants. | this study attempted to develop a list of priority pathogens. it is part of a european union (eu) project dedicated to the surveillance of emerging or re-emerging pathogens of wildlife. partners of the consortium established an initial list of 138 pathogens of concern, which was reduced to a smaller list of 65 pathogens likely to affect ruminants (i.e., the most costly animal group in the eu over the last 15 years). these 65 pathogens underwent a two-step, expert-based risk analysis: 92 experts ... | 2015 | 25496774 |
| identification of single nucleotide polymorphisms in toll-like receptor candidate genes associated with tuberculosis infection in water buffalo (bubalus bubalis). | toll-like receptors play a key role in innate immunity by recognizing pathogens and activating appropriate responses. pathogens express several signal molecules (pathogen-associated molecular patterns, pamps) essential for survival and pathogenicity. recognition of pamps triggers an array of anti-microbial immune responses through the induction of various inflammatory cytokines. the objective of this work was to perform a case-control study to characterize the distribution of polymorphisms in th ... | 2014 | 25496717 |
| feral ferrets (mustela furo) as hosts and sentinels of tuberculosis in new zealand. | the control and eventual eradication of bovine tuberculosis (tb) poses major challenges in new zealand, given the variety of wildlife species susceptible to tb, many of which are capable of onwards transmission of mycobacterium bovis infection. here we discuss the role of feral ferrets (mustela furo), focussing on potential transmission or risk pathways that have implications for management of tb. firstly inter-specific transmission to ferrets. ferrets scavenge potentially infected wildlife, inc ... | 2015 | 25495945 |
| dermal vγ4(+) γδ t cells possess a migratory potency to the draining lymph nodes and modulate cd8(+) t-cell activity through tnf-α production. | a large number of gamma delta t cells (γδ t cells) are located within epithelial tissues including the skin. in mice, epidermal and dermal γδ t cells consist of distinct subsets and have specific roles in cutaneous immune responses. a recent study demonstrated that γδ t cells and cutaneous dendritic cells migrate from the skin to the draining lymph nodes (lns). however, it remains unclear whether they regulate the antigen-specific immune response within the lns. herein, we investigated their pro ... | 2015 | 25493651 |
| new insights into mycobacterium bovis prevalence in wild mammals in portugal. | a survey to determine the prevalence of mycobacterium bovis in wild mammals in portugal was conducted by testing samples from hunted animals and those found dead between 2009 and 2013. in this study, we investigated 2116 wild mammals. post-mortem examinations were performed, and tissues were collected from wild mammals representing 8 families and 11 different species, with a total of 393 animals analysed. cultures were performed, and acid-fast isolates were identified by pcr. tissues were also s ... | 2016 | 25484245 |
| in vitro t-cell profile induced by bcg moreau in healthy brazilian volunteers. | tuberculosis (tb) remains the world's leading cause of morbidity and mortality. although mycobacterium bovis bacillus calmette-guérin (bcg) is the only vaccine currently in use, its efficacy is highly variable. it has been suggested that early antigenic presentation is a pivotal event leading to a better immune response in tb vaccine models. to investigate this further, we compared in vitro cell-mediated immune responses in the context of early sensitization with tb (i.e. healthy adults vaccinat ... | 2015 | 25483636 |
| multiplex-pcr for differentiation of mycobacterium bovis from mycobacterium tuberculosis complex. | we evaluated a multiplex-pcr to differentiate mycobacterium bovis from m. tuberculosis complex (mtc) by one step amplification based on simultaneous detection of pnca 169 c > g change in m. bovis and the is6110 present in mtc species. our findings showed the proposed multiplex-pcr is a very useful tool for complementation in differentiating m. bovis from other cultured mtc species. | 2014 | 25477916 |
| [granulomatous hepatitis revealing a mycobacterium bovis widespread infection following intravesical bcg therapy]. | intravesical therapy with bacillus calmette-guérin (bcg) has proved to be effective in the treatment of superficial bladder tumors. side-effects include local infections and rarely disseminated bcg infection with multiple end organ complications such as granulomatous hepatitis, pneumonitis, aortitis and bone marrow involvement. | 2015 | 25467298 |
| balb/c mice display more enhanced bcg vaccine induced th1 and th17 response than c57bl/6 mice but have equivalent protection. | it is generally assumed that the inbred mouse strains balb/c (h-2(d)) and c57bl/6 (h-2(b)) respond to mycobacterial infection with distinct polarisation of t helper responses, with c57bl/6 predisposed to th1 and balb/c to th2. we investigated this in a bcg-immunisation, mycobacterium bovis challenge model. following immunisation, lung and spleen cell cytokine responses to in vitro re-stimulation with a cocktail of seven secreted, immunogenic, recombinant mycobacterial proteins were determined. i ... | 2015 | 25467292 |
| towards harmonised procedures in wildlife epidemiological investigations: a serosurvey of infection with mycobacterium bovis and closely related agents in wild boar (sus scrofa) in switzerland. | bovine tuberculosis (btb) is a (re-)emerging disease in european countries, including switzerland. this study assesses the seroprevalence of infection with mycobacterium bovis and closely related agents in wild boar (sus scrofa) in switzerland, because wild boar are potential maintenance hosts of these pathogens. the study employs harmonised laboratory methods to facilitate comparison with the situation in other countries. eighteen out of 743 blood samples tested seropositive (2.4%, ci: 1.5-3.9% ... | 2015 | 25466577 |
| the evaluation of candidate biomarkers of cell-mediated immunity for the diagnosis of mycobacterium bovis infection in african buffaloes (syncerus caffer). | we evaluated commercially available bovine enzyme linked immunosorbent assays (elisa) and a human ip-10 elisa to measure ip-10, mig, mcp-1, mcp-2, mcp-3 and il1-ra in buffalo plasma in order to identify sensitive markers of the immune response to mycobacterium bovis-specific peptides. additionally, we found that all coding mrna sequences of these cytokines showed very high homology with their homologues in domestic cattle (97-99%) as did the derived amino acid sequences (97-99%). this high seque ... | 2014 | 25464825 |
| synthesis and evaluation of in vitro antimycobacterial activity of novel 1h-benzo[d]imidazole derivatives and analogues. | a series of novel 1h-benzo[d]imidazole derivatives and analogues (1-25) have been synthesized and evaluated for tuberculostatic activity. benzimidazoles substituted at the c-2 position with cyclohexylethyl, cyclohexylpropyl and phenylpropyl moiety or 4-phenylpyridine system were obtained. compounds 3, 4, 6 and 7 bearing halogen atoms or methyl groups at the benzimidazole system and cyclohexylethyl substituent at the c-2 position showed an excellent tuberculostatic activity against mycobacterium ... | 2015 | 25462221 |
| use of water troughs by badgers and cattle. | the frequency of visits by badgers and cattle to five water troughs was examined using motion-activated infra-red cameras in a farming landscape in northern ireland between may and july 2013. cattle visit rates varied significantly across troughs, were greatest during daylight periods, and more frequent during dry weather. badgers were recorded visiting only one of the five water troughs. these visits were recorded on 14 different nights between midnight and 0300 h and were mainly by individual ... | 2014 | 25458885 |
| target-based screen against a periplasmic serine protease that regulates intrabacterial ph homeostasis in mycobacterium tuberculosis. | mycobacterium tuberculosis (mtb) maintains its intrabacterial ph (phib) near neutrality in the acidic environment of phagosomes within activated macrophages. a previously reported genetic screen revealed that mtb loses this ability when the mycobacterial acid resistance protease (marp) gene is disrupted. in the present study, a high throughput screen (hts) of compounds against the protease domain of marp identified benzoxazinones as inhibitors of marp. a potent benzoxazinone, bo43 (6-chloro-2-(2 ... | 2015 | 25457457 |
| effect of cationic liposomes on bcg trafficking and vaccine-induced immune responses following a subcutaneous immunization in mice. | while formulating mycobacterium bovis bcg in lipid-based adjuvants has been shown to increase the vaccine's protective immunity, the biological mechanisms responsible for the enhanced potency of lipid encapsulated bcg are unknown. to assess whether mixing bcg in adjuvant increases its immunogenicity by altering post-vaccination organ distribution and persistence, mice were immunized subcutaneously with conventional bcg pasteur or bcg formulated in dda/tdb adjuvant and the bio-distribution of bcg ... | 2015 | 25448108 |
| bcg vaccination-induced long-lasting control of mycobacterium tuberculosis correlates with the accumulation of a novel population of cd4⁺il-17⁺tnf⁺il-2⁺ t cells. | mycobacterium bovis bacille calmette-guerin (bcg) is the only vaccine in use to prevent mycobacterium tuberculosis (mtb) infection. here we analyzed the protective efficacy of bcg against mtb challenges 21 or 120 days after vaccination. only after 120 days post-vaccination were mice able to efficiently induce early mtb growth arrest and maintain long-lasting control of mtb. this protection correlated with the accumulation of cd4(+) t cells expressing il-17(+)tnf(+)il-2(+). in contrast, mice chal ... | 2015 | 25448107 |
| combination radiofrequency ablation and local injection of the immunostimulant bacillus calmette-guérin induces antitumor immunity in the lung and at a distant vx2 tumor in a rabbit model. | to evaluate whether the combination of radiofrequency (rf) ablation and local injection of the immunostimulant mycobacterium bovis bacillus calmette-guérin (bcg) induces systemic antitumor immunity. | 2015 | 25443457 |
| multiple sampling and discriminatory fingerprinting reveals clonally complex and compartmentalized infections by m. bovis in cattle. | the combination of new genotyping tools and a more exhaustive sampling policy in the analysis of infection by mycobacterium tuberculosis has shown that infection by this pathogen is more complex than initially expected. mixed infections, coexistence of clonal variants from a parental strain, and compartmentalized infections are all different modalities of this clonal complexity. until recently, genotyping of mycobacterium bovis in animal populations was based on spoligotyping and analysis of a s ... | 2015 | 25439651 |
| why doesn't mycobacterium tuberculosis spread in animals? | 2015 | 25435136 | |
| tuberculosis vaccines and prevention of infection. | tuberculosis (tb) is a leading cause of death worldwide despite the availability of effective chemotherapy for over 60 years. although mycobacterium bovis bacillus calmette-guérin (bcg) vaccination protects against active tb disease in some populations, its efficacy is suboptimal. development of an effective tb vaccine is a top global priority that has been hampered by an incomplete understanding of protective immunity to tb. thus far, preventing tb disease, rather than infection, has been the p ... | 2014 | 25428938 |
| interferon-γ release assays for diagnosis of tuberculosis infection and disease in children. | tuberculosis (tb) remains an important problem among children in the united states and throughout the world. although diagnosis and treatment of infection with mycobacterium tuberculosis (also referred to as latent tuberculosis infection [ltbi] or tb infection) remain the lynchpins of tb prevention, there is no diagnostic reference standard for ltbi. the tuberculin skin test (tst) has many limitations, including difficulty in administration and interpretation, the need for a return visit by the ... | 2014 | 25422024 |
| attrition of t-cell functions and simultaneous upregulation of inhibitory markers correspond with the waning of bcg-induced protection against tuberculosis in mice. | mycobacterium bovis bacille calmette-guérin (bcg) is the most widely used live attenuated vaccine. however, the correlates of protection and waning of its immunity against tuberculosis is poorly understood. in this study, we correlated the longitudinal changes in the magnitude and functional quality of cd4(+) and cd8(+) t-cell response over a period of two years after mucosal or parenteral bcg vaccination with the strength of protection against mycobacterium tuberculosis in mice. the bcg vaccina ... | 2014 | 25419982 |
| comparative functional genomics and the bovine macrophage response to strains of the mycobacterium genus. | mycobacterial infections are major causes of morbidity and mortality in cattle and are also potential zoonotic agents with implications for human health. despite the implementation of comprehensive animal surveillance programs, many mycobacterial diseases have remained recalcitrant to eradication in several industrialized countries. two major mycobacterial pathogens of cattle are mycobacterium bovis and mycobacterium avium subspecies paratuberculosis (map), the causative agents of bovine tubercu ... | 2014 | 25414700 |
| biochemical and structural characterization of mycobacterial aspartyl-trna synthetase asps, a promising tb drug target. | the human pathogen mycobacterium tuberculosis is the causative agent of pulmonary tuberculosis (tb), a disease with high worldwide mortality rates. current treatment programs are under significant threat from multi-drug and extensively-drug resistant strains of m. tuberculosis, and it is essential to identify new inhibitors and their targets. we generated spontaneous resistant mutants in mycobacterium bovis bcg in the presence of 10× the minimum inhibitory concentration (mic) of compound 1, a pr ... | 2014 | 25409504 |
| molecular confirmation of bacillus calmette guerin vaccine related adverse events among saudi arabian children. | bacillus calmette guerin (bcg) is the only available vaccine for tuberculosis (tb). low grade complications in healthy recipients and disseminated vaccine associated complications among immuno-suppressed individuals were noticed globally after administration. recently a series of clinically suspected bcg associated suppurative and non-suppurative lymphadenitis cases were reported from different regions of saudi arabia. however a molecular confirmative analysis was lacking to prove these claims. | 2014 | 25409184 |
| prevalence of bovine tuberculosis in egyptian cattle and the standardization of the interferon-gamma assay as an ancillary test. | bovine tuberculosis (btb) caused primarily by mycobacterium bovis continues to cause significant losses in the cattle industry and is a major public health problem. despite its worldwide application, the ifn-γ assay has not been applied in egypt. the aim of this study was to determine the appropriate cut-off value of ifn-γ assay to complement the skin test screening in egypt. the relative sensitivity (ser ) of ppd and antigen cocktail-based ifn-γ assays (ifn-γ-ba and ifn-γ-ec) was analysed retro ... | 2016 | 25408173 |
| variance components for susceptibility to mycobacterium bovis infection in dairy and beef cattle. | infection of livestock with bovine tuberculosis (btb; mycobacterium bovis) is of major economical concern in many countries; approximately 15 000 to 20 000 cattle are infected per year in ireland. the objective of this study was to quantify the genetic variation for btb susceptibility in irish dairy and beef cattle. | 2014 | 25407624 |
| improved detection of mycobacterium bovis in bovine tissues using immunomagnetic separation approaches. | immunomagnetic separation (ims) represents a simple but effective method of selectively capturing and concentrating mycobacterium bovis, the causative agent of bovine tuberculosis (btb), from tissue samples. it is a physical cell separation technique that does not impact cell viability, unlike traditional chemical decontamination prior to culture. ims is performed with paramagnetic beads coated with m. bovis-specific antibody and peptide binders. once captured by ims, m. bovis cells can be detec ... | 2015 | 25399094 |
| a new recombinant bcg vaccine induces specific th17 and th1 effector cells with higher protective efficacy against tuberculosis. | tuberculosis (tb) is an infectious disease caused by mycobacterium tuberculosis (mtb) that is a major public health problem. the vaccine used for tb prevention is mycobacterium bovis bacillus calmette-guérin (bcg), which provides variable efficacy in protecting against pulmonary tb among adults. consequently, several groups have pursued the development of a new vaccine with a superior protective capacity to that of bcg. here we constructed a new recombinant bcg (rbcg) vaccine expressing a fusion ... | 2014 | 25398087 |
| differential mycobacterium bovis bcg vaccine-derived efficacy in c3heb/fej and c3h/heouj mice exposed to a clinical strain of mycobacterium tuberculosis. | the global epidemic caused by the bacterial pathogen mycobacterium tuberculosis continues unabated. moreover, the only available vaccine against tuberculosis, mycobacterium bovis bacillus calmette-guérin (bcg), demonstrates variable efficacy. to respond to this global threat, new animal models that mimic the pathological disease process in humans are required for vaccine testing. one new model, susceptible c3heb/fej mice, is similar to human tuberculosis in that these animals are capable of form ... | 2015 | 25392011 |
| impact of protein domains on pe_pgrs30 polar localization in mycobacteria. | pe_pgrs proteins are unique to the mycobacterium tuberculosis complex and a number of other pathogenic mycobacteria. pe_pgrs30, which is required for the full virulence of m. tuberculosis (mtb), has three main domains, i.e. an n-terminal pe domain, repetitive pgrs domain and the unique c-terminal domain. to investigate the role of these domains, we expressed a gfp-tagged pe_pgrs30 protein and a series of its functional deletion mutants in different mycobacterial species (mtb, mycobacterium bovis ... | 2014 | 25390359 |
| severity of bovine tuberculosis is associated with co-infection with common pathogens in wild boar. | co-infections with parasites or viruses drive tuberculosis dynamics in humans, but little is known about their effects in other non-human hosts. this work aims to investigate the relationship between mycobacterium bovis infection and other pathogens in wild boar (sus scrofa), a recognized reservoir of bovine tuberculosis (btb) in mediterranean ecosystems. for this purpose, it has been assessed whether contacts with common concomitant pathogens are associated with the development of severe btb le ... | 2014 | 25350002 |
| mycobacterium caprae infection in humans. | mycobacterium caprae, a member of the mycobacterium tuberculosis complex, causes tuberculosis (tb) in man and animals. some features distinguish m. caprae from its epidemiological twin, mycobacterium bovis: m. caprae is evolutionarily older, accounts for a smaller burden of zoonotic tb and is not globally distributed, but primarily restricted to european countries. m. caprae occurs only in a low proportion of human tb cases and this proportion may even decrease, if progress toward eradication of ... | 2014 | 25345680 |
| development of a new tuberculosis vaccine: is there value in the mucosal approach? | tb is a global health problem, killing 1.5 million people every year. the only currently available vaccine, mycobacterium bovis bcg, is effective against severe childhood forms, but it demonstrates a variable efficacy against the pulmonary form of tb in adults. many of these adult tb cases result from the reactivation of an initially controlled, latent mycobacterium tuberculosis infection. effective prophylactic vaccination remains the key long-term strategy for combating tb. continued belief in ... | 2014 | 25341121 |
| mucosal-associated invariant t-cells: new players in anti-bacterial immunity. | mucosal-associated invariant t (mait) cells are an innate-like t-cell population involved in anti-bacterial immunity. in human beings, mait cells are abundant, comprising ~10% of the cd8(+) t-cell compartment in blood. they are enriched at mucosal sites and are particularly prevalent within the liver. mait cells are defined by the expression of a semi-invariant t-cell receptor (vα7.2-jα33/12/20) and are restricted by the non-polymorphic, highly evolutionarily conserved mhc class ib molecule, mhc ... | 2014 | 25339949 |
| tuberculous pleuritis secondary to mycobacterium bovis in a veterinarian. | mycobacterium bovis is a rare cause of tuberculosis in humans, but should be considered in individuals at risk secondary to medical comorbidities (notably immunocompromise) or occupational exposure. most cases are secondary to reactivation of latent infection in elderly individuals although cases of primary infection still occur, usually involving animal-to-human transmission. pleural fluid culture in the context of suspected tuberculous pleuritis is frequently negative and pleural biopsy signif ... | 2016 | 25335782 |
| macrophage-inducible c-type lectin mincle-expressing dendritic cells contribute to control of splenic mycobacterium bovis bcg infection in mice. | the macrophage-inducible c-type lectin mincle has recently been identified to be a pattern recognition receptor sensing mycobacterial infection via recognition of the mycobacterial cell wall component trehalose-6',6-dimycolate (tdm). however, its role in systemic mycobacterial infections has not been examined so far. mincle-knockout (ko) mice were infected intravenously with mycobacterium bovis bcg to mimic the systemic spread of mycobacteria under defined experimental conditions. after intraven ... | 2015 | 25332121 |
| key hub and bottleneck genes differentiate the macrophage response to virulent and attenuated mycobacterium bovis. | mycobacterium bovis is an intracellular pathogen that causes tuberculosis in cattle. following infection, the pathogen resides and persists inside host macrophages by subverting host immune responses via a diverse range of mechanisms. here, a high-density bovine microarray platform was used to examine the bovine monocyte-derived macrophage transcriptome response to m. bovis infection relative to infection with the attenuated vaccine strain, m. bovis bacille calmette-guérin. differentially expres ... | 2014 | 25324841 |
| mrp8/14 induces autophagy to eliminate intracellular mycobacterium bovis bcg. | to explore the role of myeloid-related protein 8/14 in mycobacterial infection. | 2015 | 25312864 |
| [screening and identification of c7c peptide ligands of mycobacterium tuberculosis]. | to obtain the c7c peptide ligands of mycobacterium tuberculosis by affinity screening based on the phage-displayed random c7c peptide library, and preliminarily identify the binding capacity of the peptide to mycobacterium. | 2014 | 25312572 |
| orchestration of pulmonary t cell immunity during mycobacterium tuberculosis infection: immunity interruptus. | despite the introduction almost a century ago of mycobacterium bovis bcg (bcg), an attenuated form of m. bovis that is used as a vaccine against mycobacterium tuberculosis, tuberculosis remains a global health threat and kills more than 1.5 million people each year. this is mostly because bcg fails to prevent pulmonary disease--the contagious form of tuberculosis. although there have been significant advances in understanding how the immune system responds to infection, the qualities that define ... | 2014 | 25311810 |
| virulence of two strains of mycobacterium bovis in cattle following aerosol infection. | over the past two decades, highly virulent strains of mycobacterium tuberculosis have emerged and spread rapidly in man, suggesting a selective advantage based on virulence. a similar scenario has not been described for mycobacterium bovis infection in cattle (i.e. bovine tuberculosis). an epidemiological investigation of a recent outbreak of bovine tuberculosis in a usa dairy indicated that the causative strain of m. bovis (strain 10-7428) was particularly virulent, with rapid spread within the ... | 2014 | 25306158 |
| genetic diversity and dynamic distribution of mycobacterium tuberculosis isolates causing pulmonary and extrapulmonary tuberculosis in thailand. | this study examined the genetic diversity and dynamicity of circulating mycobacterium tuberculosis strains in thailand using nearly neutral molecular markers. the single nucleotide polymorphism (snp)-based genotypes of 1,414 culture-positive m. tuberculosis isolates from 1,282 pulmonary tuberculosis (ptb) and 132 extrapulmonary tb (eptb) patients collected from 1995 to 2011 were characterized. among the eight snp cluster groups (scg), scg2 (44.1%), which included the beijing (bj) genotype, and s ... | 2014 | 25297330 |
| disseminated bacillus calmette-guerin infections after intravesical therapy. | intravesical instillation of bacillus calmette-guerin (bcg) is the treatment of choice for superficial bladder carcinoma. disseminated bcg infection presenting as granulomatous hepatitis or pneumonitis is a very rare complication of this treatment. here we report a case series of seven patients previously treated with bcg presenting with pneumonitis. in two of the cases, identification of mycobacterium bovis was achieved with molecular methods. | 2014 | 25297033 |
| the epidemiology of mycobacterium bovis in wild deer and feral pigs and their roles in the establishment and spread of bovine tuberculosis in new zealand wildlife. | in new zealand, wild deer and feral pigs are assumed to be spillover hosts for mycobacterium bovis, and so are not targeted in efforts aimed at locally eradicating bovine tuberculosis (tb) from possums (trichosurus vulpecula), the main wildlife host. here we review the epidemiology of tb in deer and pigs, and assess whether new zealand's tb management programme could be undermined if these species sometimes achieve maintenance host status. in new zealand, tb prevalences of up to 47% have been re ... | 2015 | 25295713 |
| epidemiology and control of mycobacterium bovis infection in brushtail possums (trichosurus vulpecula), the primary wildlife host of bovine tuberculosis in new zealand. | the introduced australian brushtail possum (trichosurus vulpecula) is a maintenance host for bovine tuberculosis (tb) in new zealand and plays a central role in the tb problem in this country. the tb-possum problem emerged in the late 1960s, and intensive lethal control of possums is now used to reduce densities to low levels over 8 million ha of the country. this review summarises what is currently known about the pathogenesis and epidemiology of tb in possums, and how the disease responds to p ... | 2015 | 25290902 |
| gene deletions in mycobacterium bovis bcg stimulate increased cd8+ t cell responses. | mycobacteria, the etiological agents of tuberculosis and leprosy, have coevolved with mammals for millions of years and have numerous ways of suppressing their host's immune response. it has been suggested that mycobacteria may contain genes that reduce the host's ability to elicit cd8(+) t cell responses. we screened 3,290 mutant mycobacterium bovis bacillus calmette guerin (bcg) strains to identify genes that decrease major histocompatibility complex (mhc) class i presentation of mycobacterium ... | 2014 | 25287928 |
| mycobacterium bovis in burkina faso: epidemiologic and genetic links between human and cattle isolates. | in sub-saharan africa, bovine tuberculosis (btb) is a potential hazard for animals and humans health. the goal of this study was to improve our understanding of btb epidemiology in burkina faso and especially mycobacterium bovis transmission within and between the bovine and human populations. | 2014 | 25275305 |
| toward eradication: the effect of mycobacterium bovis infection in wildlife on the evolution and future direction of bovine tuberculosis management in new zealand. | new zealand's bovine tuberculosis (tb) control programme has greatly reduced the burden of tuberculosis on the farming industry, from 11% of mature cattle found with tb at slaughter in 1905 to <0.003% in 2012/13. new zealand implemented tb control measures in cattle from the mid-twentieth century, and later in farmed deer. control was based on established methods of tuberculin testing of herds, slaughter of suspect cases, and livestock movement control. unexplained regional control failures and ... | 2015 | 25273888 |
| performance of the sd bioline tb ag mpt64 rapid test for quick confirmation of mycobacterium bovis isolates from animals. | mycobacterium (m.) bovis, a bacterium in the m. tuberculosis complex, is a causative agent of bovine tuberculosis, a contagious disease of animals. mycobacterial culture is the gold standard for diagnosing bovine tuberculosis, but this technique is laborious and time-consuming. in the present study, performance of the sd bioline tb ag mpt4 rapid test, an immunochromatographic assay, was evaluated using reference bacterial strains and m. bovis field isolates collected from animals. the sd mpt64 r ... | 2015 | 25269718 |
| mycobacterium tuberculosis requires the ecf sigma factor sige to arrest phagosome maturation. | sige represents one of the best characterized alternative sigma factors of mycobacterium tuberculosis, playing a major role in the response to several environmental stresses and essential for growth in macrophages and virulence. in previous work we demonstrated that a mutant of m. tuberculosis in which the sige gene was disrupted by a cassette conferring hygromycin resistance is a promising vaccine candidate conferring better protection than mycobacterium bovis bcg in a mouse model of infection. ... | 2014 | 25268826 |
| sources of bovine tuberculosis in the united states. | despite control and eradication efforts, bovine tuberculosis continues to be identified at low levels among cattle in the united states. we evaluated possible external sources of infection by characterizing the genetic relatedness of bovine tuberculosis from a national database of reported infections, comparing strains circulating among us cattle with those of imported cattle, and farmed and wild cervids. farmed cervids maintained a genetically distinct mycobacterium bovis strain, and cattle occ ... | 2014 | 25264189 |
| development of integrated surveillance systems for the management of tuberculosis in new zealand wildlife. | disease surveillance for the management of bovine tuberculosis (tb) in new zealand has focussed, to a large extent, on the development of tools specific for monitoring mycobacterium bovis infection in wildlife. diagnostic techniques have been modified progressively over 30 years of surveillance of tb in wildlife, from initial characterisation of gross tb lesions in a variety of wildlife, through development of sensitive culture techniques to identify viable mycobacteria, to molecular identificat ... | 2015 | 25263814 |
| long-term monitoring of 10 selected pathogens in wild boar (sus scrofa) in sierra nevada national park, southern spain. | wild boar (sus scrofa) populations are increasing in the iberian peninsula, and population management must include disease management and control. in this study, the epidemiology of 10 selected pathogens (aujeszky's disease virus - adv, porcine reproductive and respiratory syndrome virus - prrsv, porcine influenza virus, porcine circovirus, porcine parvovirus, erysipelotrix rhusiopathiae, leptospira pomona, chlamydia/chlamydiaceae sp., salmonella sp. and mycobacterium bovis) in the wild boar pop ... | 2014 | 25261921 |
| improving mycobacterium bovis bacillus calmette-guèrin as a vaccine delivery vector for viral antigens by incorporation of glycolipid activators of nkt cells. | recombinant mycobacterium bovis bacillus calmette-guèrin (rbcg) has been explored as a vector for vaccines against hiv because of its ability to induce long lasting humoral and cell mediated immune responses. to maximize the potential for rbcg vaccines to induce effective immunity against hiv, various strategies are being employed to improve its ability to prime cd8+ t cells, which play an important role in the control of hiv infections. in this study we adopted a previously described approach o ... | 2014 | 25255287 |
| occurrence of mycobacterium bovis and non-tuberculous mycobacteria (ntm) in raw and pasteurized milk in the northwestern region of paraná, brazil. | milk is widely consumed in brazil and can be the vehicle of agent transmission. in this study, was evaluated the occurrence of mycobacterium bovis and non-tuberculous mycobacteria (ntm) in raw and pasteurized milk consumed in the northwestern region of paraná, brazil. fifty-two milk samples (20 pasteurized and 32 raw) from dairy farms near the municipality of maringa, parana state, brazil were collected. milk samples were decontaminated using 5% oxalic acid method and cultured on lowenstein-jens ... | 2014 | 25242962 |
| molecular typing of mycobacterium bovis isolated in the south of brazil. | bovine tuberculosis is a major infectious disease of the cattle. in this study, 85 m. bovis isolates from 162 lymph nodes, obtained from a herd of cattle on a farm in southern brazil, were evaluated using spoligotyping and vntr. the strains were grouped into five clusters and five orphans, showing a heterogenic genetic profile, what could represent diverse geographic origins of the introduced cows and/or the frequent movement of cattle between different properties. | 2014 | 25242955 |
| molecular typing of mycobacterium bovis isolates: a review. | mycobacterium bovis is the main causative agent of animal tuberculosis (tb) and it may cause tb in humans. molecular typing of m. bovis isolates provides precise epidemiological data on issues of inter- or intra-herd transmission and wildlife reservoirs. techniques used for typing m. bovis have evolved over the last 2 decades, and pcr-based methods such as spoligotyping and mycobacterial interspersed repetitive unit-variable number tandem repeat (miru-vntr) have been extensively used. these tech ... | 2014 | 25242917 |
| antimycobacterial evaluation of novel [4,5-dihydro-1h-pyrazole-1-carbonyl]pyridine derivatives synthesized by microwave-mediated michael addition. | the focus of this study is the synthesis and biological activity evaluation of a series of dibenzalaceton derivatives (3a-3n) and novel [4,5-dihydro-1h-pyrazole-1-carbonyl]pyridine derivatives (5a-5g) against mycobacterium bovis, bacillus calmette-guerin (bcg). dibenzalacetone derivatives were synthesized by benzaldehyde derivatives. the [4,5-dihydro-1h-pyrazole-1-carbonyl]pyridine derivatives were synthesized by michael addition reaction and using green chemistry microwave-mediated method. all ... | 2015 | 25219796 |
| prolactin modulates cytokine production induced by culture filtrate proteins of m. bovis through different signaling mechanisms in thp1 cells. | the immunomodulatory functions of prolactin (prl) are well recognized. augmented prl plasma levels were observed in patients with advanced tuberculosis (tb). recently, we have reported that lps and mycobacterium bovis (m. bovis) induced differential expression of prl receptor (prlr) isoforms in thp-1 cells and bovine macrophages, respectively. the aim of this work was to determine whether prl should be considered as a potential modulator of the signaling pathways and cytokine synthesis, induced ... | 2015 | 25218920 |
| neutrophils sense microbe size and selectively release neutrophil extracellular traps in response to large pathogens. | neutrophils are critical for antifungal defense, but the mechanisms that clear hyphae and other pathogens that are too large to be phagocytosed remain unknown. we found that neutrophils sensed microbe size and selectively released neutrophil extracellular traps (nets) in response to large pathogens, such as candida albicans hyphae and extracellular aggregates of mycobacterium bovis, but not in response to small yeast or single bacteria. nets were fundamental in countering large pathogens in vivo ... | 2014 | 25217981 |
| immunodeficiency associated with a nonsense mutation of ikbkb. | we report an infant of consanguineous parents of turkish decent with a novel immunodeficiency associated with homozygosity for a nonsense mutation of the gene encoding inhibitor of nuclear factor kappa-b (nf-κb) kinase subunit beta (ikkβ). at five months, she presented with respiratory insufficiency and pneumocystis jirovecii pneumonia which was successfully treated. at nine months, iatrogenic systemic infection with mycobacterium bovis was found and eventually led to her death at age 14 months. ... | 2014 | 25216719 |
| impact of in vitro evolution on antigenic diversity of mycobacterium bovis bacillus calmette-guerin (bcg). | mycobacterium bovis bacillus calmette-guerin (bcg), the only vaccine currently used against tuberculosis, is an attenuated derivative of m. bovis that has been propagated in vitro for more than 40 years. we have previously reported that the experimentally-verified human t cell epitopes of the m. tuberculosis complex (mtbc) are the most conserved elements of the genome; whether immune recognition is the force driving the conservation of epitopes in the mtbc is unknown. therefore, we sequenced the ... | 2014 | 25211768 |
| mycobacterium bovis bcg promotes tumor cell survival from tumor necrosis factor-α-induced apoptosis. | increased incidence of lung cancer among pulmonary tuberculosis patients suggests mycobacteria-induced tumorigenic response in the host. the alveolar epithelial cells, candidate cells that form lung adenocarcinoma, constitute a niche for mycobacterial replication and infection. we thus explored the possible mechanism of m. bovis bacillus calmette-guérin (bcg)-assisted tumorigenicity in type ii epithelial cells, human lung adenocarcinoma a549 and other cancer cells. | 2014 | 25208737 |
| rna-seq transcriptional profiling of peripheral blood leukocytes from cattle infected with mycobacterium bovis. | bovine tuberculosis, caused by infection with mycobacterium bovis, is a major endemic disease affecting cattle populations worldwide, despite the implementation of stringent surveillance and control programs in many countries. the development of high-throughput functional genomics technologies, including gene expression microarrays and rna-sequencing (rna-seq), has enabled detailed analysis of the host transcriptome to m. bovis infection, particularly at the macrophage and peripheral blood level ... | 2014 | 25206354 |
| bovine tuberculosis surveillance in cattle and free-ranging wildlife in eu member states in 2013: a survey-based review. | bovine tuberculosis (tb) is a common disease in cattle and wildlife, with animal health, zoonotic and economic impacts. most of the tb data for the european union (eu) concern the epidemiological situation, but comprehensive descriptions of the way in which surveillance is conducted in each country are rare, despite being essential for cross-europe comparisons. a european survey was conducted in the 28 member states and in three other neighboring countries (norway, macedonia and switzerland), to ... | 2014 | 25205200 |
| [pleural and peritoneal tuberculosis due to mycobacterium bovis]. | 2015 | 25200214 | |
| overexpression of the salmonella kdpf membrane peptide modulates expression of kdp genes and intramacrophage growth. | membrane peptides appear as an emerging class of regulatory molecules in bacteria, which can interact with membrane proteins, including transporters and sensor kinases. the kdpf peptide, which is cotranscribed with kdpabc genes and regulated by the kdpde two-component system, is supposed to stabilize the kdpabc potassium transporter complex but may also exhibit unsuspected regulatory function(s). the mycobacterial kdpf can interact with the kdpd histidine kinase, and kdpf overexpression has been ... | 2014 | 25197761 |
| humoral and lung immune responses to mycobacterium tuberculosis infection in a primate model of protection (.) | recently we reported (mehra et al., 2013), that lung granulomas from mycobacterium bovis bacille calmette-guérin (bcg)-vaccinated cynomolgus macaques exhibit upon challenge with m. tuberculosis a more balanced expression of α- and β-chemokines, relative to comparable samples from sham-vaccinated animals by comparative transcriptomics. here, we studied the recruitment of immune cells to blood and lungs in m. tuberculosis-infected macaques as a function of prior bcg-vaccination. vaccination initia ... | 2014 | 25197327 |
| rapid identification of veterinary-relevant mycobacterium tuberculosis complex species using 16s rdna, is6110 and regions of difference-targeted dual-labelled hydrolysis probes. | members of the mycobacterium tuberculosis complex (mtc) are causative agents of tuberculosis (tb) in both humans and animals. mtc species are genetically very similar but may differ in their epidemiology, namely geographic distribution and host preferences, virulence traits and antimicrobial susceptibility patterns. however, the conventional laboratory diagnosis does not routinely differentiate between the species of the mtc. in this work we describe a rapid and robust two-step five-target probe ... | 2014 | 25192844 |
| effects of mycobacteria major secretion protein, ag85b, on allergic inflammation in the lung. | many epidemiological studies have suggested that the recent increase in prevalence and severity of allergic diseases such as asthma is inversely correlated with mycobacterium bovis bacillus calmette guerin (bcg) vaccination. however, the underlying mechanisms by which mycobacterial components suppress allergic diseases are not yet fully understood. here we showed the inhibitory mechanisms for development of allergic airway inflammation by using highly purified recombinant ag85b (rag85b), which i ... | 2014 | 25192550 |
| disseminated bacillus calmette guerin disease in a twin infant with severe combined immunodeficiency disease. | fatal-disseminated bacillus calmette guerin (bcg) disease is well known in infants with severe combined immunodeficiency after bcg vaccination. we report a 7 month male infant delivered as a product of in vitro fertilization and twin gestation that presented with fever, cough and multiple nodular skin lesions. a biopsy of skin lesions revealed the presence of acid fast bacilli. mycobacterium bovis infection was confirmed by polymerase chain reaction (pcr) and molecular studies. immunological pro ... | 2014 | 25191057 |