Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| senp1 regulates ifn-γ-stat1 signaling through stat3-socs3 negative feedback loop. | interferon-γ (ifn-γ) triggers macrophage for inflammation response by activating the intracellular jak-stat1 signaling. suppressor of cytokine signaling 1 (socs1) and protein tyrosine phosphatases can negatively modulate ifn-γ signaling. here, we identify a novel negative feedback loop mediated by stat3-socs3, which is tightly controlled by senp1 via de-sumoylation of protein tyrosine phosphatase 1b (ptp1b), in ifn-γ signaling. senp1-deficient macrophages show defects in ifn-γ signaling and m1 m ... | 2016 | 27702761 |
| preparation and characterization of agar-based nanocomposite films reinforced with bimetallic (ag-cu) alloy nanoparticles. | agar-based active nanocomposite films were prepared by incorporating silver-copper (ag-cu) alloy nanoparticles (nps) (0.5-4wt%) into glycerol plasticized agar solution. thermo-mechanical, morphological, structural, and optical properties of the nanocomposite films were characterized by texture analyzer, differential scanning calorimetry (dsc), scanning electron microscope (sem), x-ray diffraction (xrd), fourier transforms infrared (ftir) spectroscopy, and surface color measurement. tensile stren ... | 2017 | 27702525 |
| the comparing of antimicrobial activity of csn1s2 protein of fresh milk and yoghurt goat breed ethawah inhibited the pathogenic bacteria. | goat milk is reported to have antimicrobial activity of several pathogen bacteria that contained on food materials. the research related with antimicrobial activity of alpha-s2 casein from goat milk is relatively less than other casein components. herein, we reported the antimicrobial activity of caprine alpha-s2 casein (csn1s2) protein from ethawah breed goat milk and yoghurt in gram positive (listeria monocytogenes, staphylococcus aureus and bacillus cereus) and negative pathogen bacteria (esc ... | 2016 | 27698594 |
| microfluidic cantilever detects bacteria and measures their susceptibility to antibiotics in small confined volumes. | in the fight against drug-resistant bacteria, accurate and high-throughput detection is essential. here, a bimaterial microcantilever with an embedded microfluidic channel with internal surfaces chemically or physically functionalized with receptors selectively captures the bacteria passing through the channel. bacterial adsorption inside the cantilever results in changes in the resonance frequency (mass) and cantilever deflection (adsorption stress). the excitation of trapped bacteria using inf ... | 2016 | 27698375 |
| influence of pdo ragusano cheese biofilm microbiota on flavour compounds formation. | the objectives of the present study were to characterize the biofilm microbiota of 11 different farms (from a to k), producing pdo ragusano cheese, and to investigate on its ability to generate volatile organic compounds (vocs) in milk samples inoculated with biofilm and incubated under ragusano cheese making conditions. the biofilms were subjected to plate counting and pcr/t/dgge analysis and the vocs generated in incubated milk samples were evaluated through smartnose, gc/o, and gc/ms. strepto ... | 2017 | 27697162 |
| molecular analysis of the role of osmolyte transporters opuca and betl in listeria monocytogenes after cold and freezing stress. | listeria monocytogenes is a food-borne pathogen of humans and other animals. the striking ability to survive several stresses usually used for food preservation makes l. monocytogenes one of the biggest concerns to the food industry. this ubiquity can be partly explained by the ability of the organism to grow and persist at very low temperatures, a consequence of its ability to accumulate cryoprotective compound called osmolytes. a quantitative rt-pcr assay was used to measure mrna transcript ac ... | 2017 | 27695911 |
| persistence and potential viable but non-culturable state of pathogenic bacteria during storage of digestates from agricultural biogas plants. | despite the development of on-farm anaerobic digestion as a process for making profitable use of animal by-products, factors leading to the inactivation of pathogenic bacteria during storage of digestates remain poorly described. here, a microcosm approach was used to evaluate the persistence of three pathogenic bacteria (salmonella enterica derby, campylobacter coli and listeria monocytogenes) in digestates from farms, stored for later land spreading. nine samples, including raw digestates, liq ... | 2016 | 27695451 |
| intracellular organisms as placental invaders. | in this article we present a novel model for how the human placenta might get infected via the hematogenous route. we present a list of diverse placental pathogens, like listeria monocytogenes or cytomegalovirus, which are familiar to most obstetricians, but others, like salmonella typhi, have only been reported in case studies or small case series. remarkably, all of these organisms on this list are either obligate or facultative intracellular organisms. these pathogens are able to enter and su ... | 2015 | 27695204 |
| distinct surveillance pathway for immunopathology during acute infection via autophagy and sr-bi. | the mechanisms protecting from immunopathology during acute bacterial infections are incompletely known. we found that in response to apoptotic immune cells and live or dead listeria monocytogenes scavenger receptor bi (sr-bi), an anti-atherogenic lipid exchange mediator, activated internalization mechanisms with characteristics of macropinocytosis and, assisted by golgi fragmentation, initiated autophagic responses. this was supported by scavenger receptor-induced local increases in membrane ch ... | 2016 | 27694929 |
| listeria monocytogenes in stone fruits linked to a multistate outbreak: enumeration of cells and whole-genome sequencing. | in 2014, the identification of stone fruits contaminated with listeria monocytogenes led to the subsequent identification of a multistate outbreak. simultaneous detection and enumeration of l. monocytogenes were performed on 105 fruits, each weighing 127 to 145 g, collected from 7 contaminated lots. the results showed that 53.3% of the fruits yielded l. monocytogenes (lower limit of detection, 5 cfu/fruit), and the levels ranged from 5 to 2,850 cfu/fruit, with a geometric mean of 11.3 cfu/fruit ... | 2016 | 27694232 |
| widespread shortening of 3' untranslated regions and increased exon inclusion are evolutionarily conserved features of innate immune responses to infection. | the contribution of pre-mrna processing mechanisms to the regulation of immune responses remains poorly studied despite emerging examples of their role as regulators of immune defenses. we sought to investigate the role of mrna processing in the cellular responses of human macrophages to live bacterial infections. here, we used mrna sequencing to quantify gene expression and isoform abundances in primary macrophages from 60 individuals, before and after infection with listeria monocytogenes and ... | 2016 | 27690314 |
| effect of lemongrass essential oil vapors on microbial dynamics and listeria monocytogenes survival on rocket and melon stored under different packaging conditions and temperatures. | the aim of the present study was to examine the effect of lemongrass essential oil vapors on the dynamics of surface microbiota and l. monocytogenes growth on rocket and melon under different packaging conditions and storage temperature. for that purpose, rocket and melon were placed on expanded polystyrene (eps) trays, sprayed with l. monocytogenes to a population of 4.5-5.0 log cfu·g(-1), packaged using microperforated oriented polypropylene (opp) film in either air or microperforated active m ... | 2015 | 27682104 |
| comparison of microbiological and probiotic characteristics of lactobacilli isolates from dairy food products and animal rumen contents. | lactobacilli are employed in probiotic food preparations and as feed additives in poultry and livestock, due to health benefits associated with their consumption. the objective of this study was to evaluate and compare the probiotic potential of ten lactobacilli strains isolated from commercial dairy food products and animal rumen contents in new zealand. genetic identification of the isolates revealed that all belonged to the genus lactobacillus, specifically the species l. reuteri, l. rhamnosu ... | 2015 | 27682086 |
| isolation and taxonomic identity of bacteriocin-producing lactic acid bacteria from retail foods and animal sources. | bacteriocin-producing (bac⁺) lactic acid bacteria (lab) were isolated from a variety of food products and animal sources. samples were enriched in de man, rogosa, and sharpe (mrs) lactocilli broth and plated onto mrs agar plates using a "sandwich overlay" technique. inhibitory activity was detected by the "deferred antagonism" indicator overlay method using listeria monocytogenes as the primary indicator organism. antimicrobial activity against l. monocytogenes was detected by 41 isolates obtain ... | 2015 | 27682080 |
| fumaric acid and slightly acidic electrolyzed water inactivate gram positive and gram negative foodborne pathogens. | sanitizing effectiveness of slightly acidic electrolyzed water (saew) and fumaric acid (fa) at different dipping temperatures (25-60 °c), times (1-5 min), and concentrations (5-30 ppm for saew and 0.125%-0.5% for fa) on pure cultures of two gram positive pathogens staphylococcus aureus (sa) and listeria monocytogenes (lm) and two gram negative pathogens escherichia coli o157:h7 (ec) and salmonella typhimurium (st) was evaluated. fa (0.25%) showed the strongest sanitizing effect, demonstrating co ... | 2015 | 27682077 |
| identification of multiple bacteriocins in enterococcus spp. using an enterococcus-specific bacteriocin pcr array. | twenty-two bacteriocin-producing enterococcus isolates obtained from food and animal sources, and demonstrating activity against listeria monocytogenes, were screened for bacteriocin-related genes using a bacteriocin pcr array based on known enterococcal bacteriocin gene sequences in the ncbi genbank database. the 22 bacteriocin-positive (bac+) enterococci included en. durans (1), en. faecalis (4), en. faecium (12), en. hirae (3), and en. thailandicus (2). enterocin a (enta), enterocins mr10a an ... | 2015 | 27682075 |
| a look inside the listeria monocytogenes biofilms extracellular matrix. | listeria monocytogenes is a foodborne pathogen able to persist in food industry and is responsible for a severe illness called listeriosis. the ability of l. monocytogenes to persist in environments is due to its capacity to form biofilms that are a sessile community of microorganisms embedded in a self-produced matrix of extracellular polymeric substances (eps's). in this review, we summarized recent efforts performed in order to better characterize the polymeric substances that compose the ext ... | 2016 | 27681916 |
| phage inactivation of listeria monocytogenes on san daniele dry-cured ham and elimination of biofilms from equipment and working environments. | the anti-listerial activity of generally recognized as safe (gras) bacteriophage listex p100 (phage p100) was demonstrated in broths and on the surface of slices of dry-cured ham against 5 strains or serotypes (i.e., scott a, 1/2a, 1/2b, and 4b) of listeria monocytogenes. in a broth model system, phage p100 at a concentration equal to or greater than 7 log pfu/ml completely inhibited 2 log cfu/cm² or 3 log cfu/cm² of l. monocytogenes growth at 30 °c. the temperature (4, 10, 20 °c) seemed to infl ... | 2016 | 27681898 |
| anti-listerial activity of four seaweed essential oils against listeria monocytogenes. | listeria monocytogenes is one of the most virulent types of bacteria and causes severe foodborne illness, such as listeriosis. because this pathogen has become resistant to sanitizers and other disinfectants that are used to clean utensils and surfaces during food processing, it poses a serious threat to the food industry. | 2016 | 27679700 |
| uptake and metabolism of antibiotics roseoflavin and 8-demethyl-8-aminoriboflavin in riboflavin-auxotrophic listeria monocytogenes. | the riboflavin analogs roseoflavin (rof) and 8-demethyl-8-aminoriboflavin (af) are produced by the bacteria streptomyces davawensis and streptomyces cinnabarinus riboflavin analogs have the potential to be used as broad-spectrum antibiotics, and we therefore studied the metabolism of riboflavin (vitamin b2), rof, and af in the human pathogen listeria monocytogenes, a bacterium which is a riboflavin auxotroph. we show that the l. monocytogenes protein lmo1945 is responsible for the uptake of ribo ... | 2016 | 27672192 |
| complete genome sequence of lactobacillus plantarum lz206, a potential probiotic strain with antimicrobial activity against food-borne pathogenic microorganisms. | lactobacilli strains have been considered as important candidates for manufacturing "natural food", due to their antimicrobial properties and generally regarded as safe (gras) status. lactobacillus plantarum lz206 is a potential probiotic strain isolated from raw cow milk, with antimicrobial activity against various pathogens, including gram-positive bacteria (staphylococcus aureus and listeria monocytogenes), gram-negtive bacteria (escherichia coli and salmonella enterica), and fungus candida a ... | 2016 | 27671696 |
| a multiplex pcr for detection of listeria monocytogenes and its lineages. | a novel multiplex pcr assay was developed to identify genus listeria, and discriminate listeria monocytogenes and its major lineages (li, lii, liii). this assay is a rapid and inexpensive subtyping method for screening and characterization of l. monocytogenes. | 2016 | 27671346 |
| mitogen-activated protein kinases are required for effective infection of human choroid plexus epithelial cells by listeria monocytogenes. | listeria monocytogenes, a gram-positive bacterium, can cause meningitis after invading the human central nervous system. the blood-cerebrospinal fluid barrier (bcsfb), located at the epithelium of the choroid plexus, is a possible entry site for l. monocytogenes into the brain, and in vitro l. monocytogenes invades human choroid plexus epithelial papilloma (hibcpp) cells. although host cell signal transduction subsequent to infection by l. monocytogenes has been investigated, the role of mitogen ... | 2017 | 27671041 |
| effect of vitamin a on listeria monocytogenes infection in a silkworm model. | insect infection models have been used increasingly to study various pathogenic agents in evaluations of pathogenicity and drug efficacy. in this study, we demonstrated that larvae of the silkworm bombyx mori are useful for studying listeria monocytogenes infections in insects. infection with the l. monocytogenes wild-type strain induced silkworm death. infection by a listeriolysin o (llo) deletion mutant also induced silkworm death, but the bacterial numbers in silkworms were lower than those o ... | 2017 | 27669511 |
| manufacturers have effect on listeriae. | changes in food manufacturing and/or health education measures may have had an impact on the epidemiology of infections with listeria monocytogenes, researchers from leicester say. | 1993 | 27665816 |
| in vivo and in vitro effects of selected antioxidants on rabbit meat microbiota. | the purpose of this study was to investigate the effect of dietary vitamin e or economase™ supplementation on the growth of several background/pathogenic bacteria on rabbit carcasses and hamburgers during refrigerated storage. for 51days, 270 new zealand rabbits received either a basal diet, or experimental diets enriched with 100 or 200mg/kg of vitamin e or economase™. the bacteria studied were salmonella, listeria monocytogenes, pseudomonas, enterobacteriaceae, escherichia coli, coagulase-posi ... | 2017 | 27664938 |
| impact of starch-based emulsions on the antibacterial efficacies of nisin and thymol in cantaloupe juice. | the use of antimicrobial compounds to prevent foodborne pathogens from contaminating fresh-cut produce has received broad attentions; however, the applications of these compounds are hindered by their rapid depletion in foods. to prolong their efficacies, the use of delivery systems is essential. in this study, oil-in-water emulsions formed using starch octenyl succinate (starch-os) were used to stabilize nisin and thymol in cantaloupe juice-containing fluid. listeria monocytogenes v7 and salmon ... | 2017 | 27664621 |
| in vivo emergence of rifampicin resistance by rpob mutation in listeria monocytogenes during therapy of prosthetic joint infection. | 2016 | 27659120 | |
| normal development and function of t cells in proline rich 7 (prr7) deficient mice. | transmembrane adaptor proteins (traps) are important organisers for the transduction of immunoreceptor-mediated signals. prr7 is a trap that regulates t cell receptor (tcr) signalling and potently induces cell death when overexpressed in human jurkat t cells. whether endogenous prr7 has a similar functional role is currently unknown. to address this issue, we analysed the development and function of the immune system in prr7 knockout mice. we found that loss of prr7 partially impairs development ... | 2016 | 27657535 |
| insights into the mechanism of homeoviscous adaptation to low temperature in branched-chain fatty acid-containing bacteria through modeling fabh kinetics from the foodborne pathogen listeria monocytogenes. | the psychrotolerant foodborne pathogen listeria monocytogenes withstands the stress of low temperatures and can proliferate in refrigerated food. bacteria adapt to growth at low temperatures by increasing the production of fatty acids that increase membrane fluidity. the mechanism of homeoviscous increases in unsaturated fatty acid amounts in bacteria that predominantly contain straight-chain fatty acids is relatively well understood. by contrast the analogous mechanism in branched-chain fatty a ... | 2016 | 27656167 |
| a counterselection method for lactococcus lactis genome editing based on class iia bacteriocin sensitivity. | in this paper, we present a new counterselection method for deleting fragments from lactococcus lactis chromosome. the method uses a non-replicating plasmid vector, which integrates into the chromosome and makes the cell sensitive to bacteriocins. the integration vector carries puc ori functional in escherichia coli but not in l. lactis, an erythromycin resistance gene for selecting single crossover integrants, and two fragments from l. lactis chromosome for homologous recombinations. in additio ... | 2016 | 27654656 |
| human γδ t cells augment antigen presentation in listeria monocytogenes infection. | circulating γδ t cells in healthy individuals rapidly respond to bacterial and viral pathogens. many studies have demonstrated that γδ t cells are activated and expanded by listeria monocytogenes (l.monocytogenes), a foodborne bacterial pathogen with high fatality rates. however, the roles of γδ t cells during l.monocytogenes infection are not clear. in the present study, we characterized the morphological characteristics of phagocytosis in γδ t cells after l.monocytogenes infection using transm ... | 2016 | 27652377 |
| listeria rhombencephalitis complicating anti-tnf treatment during an acute flare of crohn's colitis. | patients with crohn's disease often require the use of immunosuppressant drugs to control disease activity. such medication includes steroids, azathioprine, and biologic therapy. these suppress the immune response, and the patient is more susceptible to infection. we present a case of a 69-year-old gentleman with a history of crohn's colitis who had ongoing symptoms of diarrhoea in spite of standard treatment. biologic therapy was considered to be the next step, and screening for infection was u ... | 2016 | 27651962 |
| rab32 restriction of intracellular bacterial pathogens. | our immune system is engaged in a continuous battle against invading pathogens, many of which have evolved to survive in intracellular niches of mammalian hosts. a variety of cellular processes are involved in preventing bacterial invasion or in killing bacteria that successfully invade host cells. recently, the rab gtpase rab32 emerged as critical regulator of a host defense pathway that can eliminate bacterial pathogens. salmonella enterica is an intracellular bacterium and a major cause of in ... | 2016 | 27645564 |
| tumor suppressor p53 protects mice against listeria monocytogenes infection. | tumor suppressor p53 is involved in regulating immune responses, which contribute to antitumor and antiviral activity. however, whether p53 has anti-bacterial functions remains unclear. listeria monocytogenes (lm) causes listeriosis in humans and animals, and it is a powerful model for studying innate and adaptive immunity. in the present study, we illustrate an important regulatory role of p53 during lm infection. p53 knockout (p53ko) mice were more susceptible to lm infection, which was manife ... | 2016 | 27644341 |
| listeria monocytogenes strains which are underrepresented during selective enrichment with the iso method might dominate during passage through simulated gastric fluid and in vitro infection of caco-2 cells. | various listeria monocytogenes strains may contaminate a single food potentially resulting in simultaneous exposure of consumers to multiple strains. however, l. monocytogenes strains isolated from foods by selective enrichment (se) might not always represent those that can better survive a patient immune system, due to bias in strain recovery. we investigated the effect of co-cultivation in tsb-y at 10°c for 8 days on i) the detection of l. monocytogenes strains during se with the iso 11290-1:1 ... | 2016 | 27637880 |
| genome sequence of the listeria monocytogenes food isolate hpb913, collected in canada in 1993. | listeria monocytogenes is a pathogenic bacterium of importance to public health and food safety agencies. we present the genome sequence of the serotype 1/2a l. monocytogenes food isolate hpb913, which was collected in canada in 1993 as part of an investigation into a sporadic case of foodborne illness. | 2016 | 27634991 |
| paper-based bipolar electrode electrochemiluminescence switch for label-free and sensitive genetic detection of pathogenic bacteria. | genetic analysis is of great importance for the detection of pathogenic bacteria. bacterial identification must become simpler, less expensive, and more rapid than the traditional methods. in this study, a low-cost, label-free, and wireless paper-based bipolar electrode electrochemiluminescence (pbpe-ecl) analysis system was constructed for the rapid and sensitive genetic detection of pathogenic bacteria. wax-screen printing was used to form hydrophilic channels on filter paper, and a carbon ink ... | 2016 | 27633711 |
| topical simvastatin as host-directed therapy against severity of cutaneous leishmaniasis in mice. | we recently demonstrated that statins mediate protection against intracellular pathogens, mycobacterium tuberculosis and listeria monocytogenes in mice. here, we investigated the immunomodulatory potential of simvastatin as a topical or systemic host-directed drug therapy in controlling inflammatory responses in an experimental mouse model of cutaneous leishmaniasis caused by leishmania major (lv39). in an ear infection model, topical application of simvastatin directly on established lesions si ... | 2016 | 27632901 |
| anti-listerial effect of selected essential oils and thymol. | the anti-listerial effect of marjoram, thyme essential oils (eos) and thymol on listeria monocytogenes inoculated chicken breast fillets was investigated. before inoculation the fillets were pretreated by washing or not under running tap water. inoculated samples were kept at 6 °c for 24 h to allow the growth of l. monocytogenes. after this, the fillets were put in marinating solutions containing salt (5%) and eos or thymol in mic/2 concentration established in vitro. total germ count (tgc) and ... | 2016 | 27630055 |
| intestinal organoids as a novel tool to study microbes-epithelium interactions. | the gut, particularly the colon, is the host of approximately 1000 bacterial species, the so-called gut microbiota. the relationship between the gut microbiota and the host is symbiotic and mutualistic, influencing many aspects of the biology of the host. this homeostatic balance can be disrupted by enteric pathogens, such as shigella flexneri or listeria monocytogenes, which are able to invade the epithelial layer and consequently subvert physiological functions. to study the host-microbe inter ... | 2016 | 27628134 |
| identification of lactic acid bacteria in fruit pulp processing byproducts and potential probiotic properties of selected lactobacillus strains. | this study aimed to identify lactic acid bacteria (lab) in byproducts of fruit (malpighia glabra l., mangifera indica l., annona muricata l., and fragaria vesca l.) pulp processing. fifty strains of lab were identified using matrix-assisted laser desorption/ionization-time of flight mass spectrometry (maldi-tof ms) and 16s rrna gene sequence (16s rrna) analysis. species belonging to lactobacillus genus were the predominant lab in all fruit pulp processing byproducts. the average congruency betwe ... | 2016 | 27625647 |
| antimicrobial resistance of listeria monocytogenes isolated from seafood and humans in iran. | fourteen listeria monocytogenes isolates previously collected from seafood (n = 7) and human patients (n = 7) were studied for their antimicrobial susceptibility against eight common antimicrobials (ampicillin, penicillin, gentamicin, streptomycin, tetracycline, trimethoprim-sulfamethoxazole, chloramphenicol, and cefotaxime). a high resistance level to ampicillin, cefotaxime (100%), and pencillin (57% in seafood isolates and 71.4% in clinical isolates) was observed in this study. however, all of ... | 2016 | 27622345 |
| the correlation between nacl adaptation and heat sensitivity of listeria monocytogenes, a foodborne pathogen through fresh and processed meat. | this study examined the relationship between nacl sensitivity and stress response of listeria monocytogenes. nine strains of l. monocytogenes (nccp10805, nccp10806, nccp10807, nccp10808, nccp10809, nccp10810, nccp10811, nccp10920 and nccp 10943) were exposed to 0%, 1%, 2% and 4% nacl, and then incubated at 60℃ for 60 min to select strains that were heat-sensitized (hs) and non-sensitized (ns) by nacl exposure. after heat challenge, l. monocytogenes strains were categorized as hs (nccp 10805, ncc ... | 2016 | 27621687 |
| effect of sodium alginate coating incorporated with nisin, cinnamomum zeylanicum, and rosemary essential oils on microbial quality of chicken meat and fate of listeria monocytogenes during refrigeration. | the present study was conducted to preserve the microbial quality of chicken meat fillets during storage time by using sodium alginate active coating solutions incorporated with different natural antimicrobials including nisin, cinnamomum zeylanicum (cinnamon), and rosemary essential oils (eos) which were added individually and in combination. the samples were stored in refrigeration condition for 15days and were analyzed for total viable count, enterobacteriaceae count, lactic acid bacteria cou ... | 2016 | 27620825 |
| investigating the effect of different treatments with lactic acid bacteria on the fate of listeria monocytogenes and staphylococcus aureus infection in galleria mellonella larvae. | the use of galleria mellonella as a model host to elucidate microbial pathogenesis and search for novel drugs and therapies has been well appreciated over the past years. however, the effect of microorganisms with functional appeal in the specific host remains scarce. the present study investigates the effect of treatment with selected lactic acid bacteria (lab) with probiotic potential, as potential protective agents by using live or heat-killed cells at 6 and 24 h prior to infection with liste ... | 2016 | 27618619 |
| comprehensive evaluation and implementation of improvement actions in butcher shops. | foodborne pathogens can cause acute and chronic diseases and produce a wide range of symptoms. since the consumption of ground beef is a risk factor for infections with some bacterial pathogens, we performed a comprehensive evaluation of butcher shops, implemented improvement actions for both butcher shops and consumers, and verified the impact of those actions implemented. a comprehensive evaluation was made and risk was quantified on a 1-100 scale as high-risk (1-40), moderate-risk (41-70) or ... | 2016 | 27618439 |
| selective photocatalytic disinfection by coupling strepminisog to the antibody catalyzed water oxidation pathway. | for several decades reactive oxygen species have been applied to water quality engineering and efficient disinfection strategies; however, these methods are limited by disinfection byproduct and catalyst-derived toxicity concerns which could be improved by selectively targeting contaminants of interest. here we present a targeted photocatalytic system based on the fusion protein strepminisog that uses light within the visible spectrum to produce reactive oxygen species at a greater efficiency th ... | 2016 | 27617441 |
| protective effect of carnobacterium spp. against listeria monocytogenes during host cell invasion using in vitro ht29 model. | the pathogenesis of listeriosis results mainly from the ability of listeria monocytogenes to attach, invade, replicate and survive within various cell types in mammalian tissues. in this work, the effect of two bacteriocin-producing carnobacterium (c. divergens v41 and c. maltaromaticum v1) and three non-bacteriocinogenic strains: (c. divergens v41c9, c. divergens 2763, and c. maltaromaticum 2762) was investigated on the reduction of l. monocytogenes scott a plaque-forming during human infection ... | 2016 | 27617232 |
| fermentation of african kale (brassica carinata) using l. plantarum bfe 5092 and l. fermentum bfe 6620 starter strains. | vegetables produced in africa are sources of much needed micronutrients and fermentation is one way to enhance the shelf life of these perishable products. to prevent post-harvest losses and preserve african leafy vegetables, lactobacillus plantarum bfe 5092 and lactobacillus fermentum bfe 6620 starter strains were investigated for their application in fermentation of african kale (brassica carinata) leaves. they were inoculated at 1×10(7)cfu/ml and grew to a maximum level of 10(8)cfu/ml during ... | 2016 | 27614122 |
| expression of surface protein lapb by a wide spectrum of listeria monocytogenes serotypes as demonstrated with anti-lapb monoclonal antibodies. | protein antigens expressed on the surface of all strains of listeria monocytogenes and absent from nonpathogenic listeria spp. are presumably useful targets for pathogen identification, detection, and isolation using specific antibodies (abs). to seek such surface proteins expressed in various strains of l. monocytogenes for diagnostic applications, we focused on a set of surface proteins known to be involved or putatively involved in l. monocytogenes virulence and identified listeria adhesion p ... | 2016 | 27613687 |
| bacterial meningitis in patients using immunosuppressive medication: a population-based prospective nationwide study. | we studied occurrence, presentation, disease course, effect of adjunctive dexamethasone, and prognosis of bacterial meningitis in patients using immunosuppressive medication. patients were selected from our nationwide, prospective cohort on community-acquired bacterial meningitis performed from march 1, 2006 through october 31, 2014. eighty-seven of 1447 episodes (6 %) of bacterial meningitis occurred in patients using immunosuppressive medication, and consisted of corticosteroids in 82 %. patie ... | 2016 | 27613024 |
| methodology for identification of pore forming antimicrobial peptides from soy protein subunits β-conglycinin and glycinin. | antimicrobial peptides (amps) inactivate microbial cells through pore formation in cell membrane. because of their different mode of action compared to antibiotics, amps can be effectively used to combat drug resistant bacteria in human health. amps can also be used to replace antibiotics in animal feed and immobilized on food packaging films. in this research, we developed a methodology based on mechanistic evaluation of peptide-lipid bilayer interaction to identify amps from soy protein. produ ... | 2016 | 27612614 |
| combination of cymbopogon citratus and allium cepa essential oils increased antibacterial activity in leafy vegetables. | cymbopogon citratus and allium cepa essential oils (eos) are rich in terpenes and sulfur compounds respectively, both with antibacterial activity and different cell targets, supporting the idea that their combination can increase their efficacy. | 2017 | 27611358 |
| draft genome sequences of 15 isolates of listeria monocytogenes serotype 1/2a, subgroup st204. | listeria monocytogenes sequence type 204 (st204) strains have been isolated from a range of food, environmental, and clinical sources in australia. this study describes the draft genome sequences of 15 isolates collected from meat and dairy associated sources. | 2016 | 27609916 |
| presence of escherichia coli o157, salmonella spp., and listeria monocytogenes in raw ovine milk destined for cheese production and evaluation of the equivalence between the analytical methods applied. | italy is one of the main producers and exporters of cheese made from unpasteurized sheep milk. since raw milk and its derived products are known sources of human infections, cheese produced from raw sheep milk could pose a microbiological threat to public health. hence, the objectives of the study were: to characterize the potential risk of the presence of pathogens escherichia coli o157, listeria monocytogenes, and salmonella in raw ovine milk destined for cheese production obtained from all th ... | 2016 | 27607835 |
| adap plays a pivotal role in cd4+ t cell activation but is only marginally involved in cd8+ t cell activation, differentiation, and immunity to pathogens. | the adhesion and degranulation promoting adaptor protein (adap) is a multifunctional scaffold involved in many different signaling pathways that are important for the function of t cells, including the inside-out and outside-in signaling of integrins, the activation of nf-κb, and the subsequent production of proinflammatory cytokines (e.g., ifn-γ and il-2). strikingly, despite its well-established role in t cells, previous studies did not distinguish between cd4(+) and cd8(+) t cells, and thus, ... | 2017 | 27605210 |
| development of a sandwich elisa for ehec o157:h7 intimin γ1. | enterohemorrhagic escherichia coli (ehec) o157:h7 is a zoonotic pathogen of worldwide importance that causes foodborne infections in humans. intimin gamma 1 (intimin γ1) is one of the most important outer membrane proteins required for ehec's intimate adhesion to epithelial cells. here, we generated a polyclonal antibody (pab) and a monoclonal antibody (mab) against intimin γ1 to develop a double antibody sandwich elisa (das-elisa) with increased sensitivity and specificity for measuring ehec o1 ... | 2016 | 27603508 |
| antibiotic susceptibility, antibacterial activity and characterisation of enterococcus faecium strains isolated from breast milk. | enterococci, which have useful biotechnological applications, produce bacteriocins, including those that exert anti-listerial activity. the present study aimed to determine the antibiotic susceptibility patterns and antimicrobial activity of enterococcus faecium strains isolated from human breast milk. the strains were identified using carbohydrate fermentation tests and ribotyping. subsequently, the antibacterial activity of the isolates was investigated, and the quantities of lactic acid and h ... | 2016 | 27602088 |
| characterization of egg white antibacterial properties during the first half of incubation: a comparative study between embryonated and unfertilized eggs. | egg white is an important contributor to the protection of eggs against bacterial contaminations during the first half of incubation (day zero to 12), prior to the egg white transfer into the amniotic fluid to be orally absorbed by the embryo. this protective system relies on an arsenal of antimicrobial proteins and on intrinsic physicochemical properties that are generally unfavorable for bacterial multiplication and dissemination. some changes in these parameters can be observed in egg white d ... | 2016 | 27601682 |
| [bacterial meningitis in adults in emergency and rescue services]. | the cardinal symptoms of bacterial meningitis are headache, fever, impaired consciousness and nuchal stiffness (meningism); however, the diagnosis of acute bacterial meningitis can only be confirmed or ruled out by investigation of cerebrospinal fluid. the recommended empirical antibiotic regimen for community-acquired acute bacterial meningitis in adults in germany is a combination of ceftriaxone and ampicillin plus adjuvant dexamethasone. an important influenceable factor for treatment success ... | 2016 | 27600937 |
| extracellular superoxide dismutase enhances recruitment of immature neutrophils to the liver. | listeria monocytogenes is a gram-positive intracellular pathogen that causes spontaneous abortion in pregnant women, as well as septicemia, meningitis, and gastroenteritis, primarily in immunocompromised individuals. although l. monocytogenes can usually be effectively treated with antibiotics, there is still around a 25% mortality rate with individuals who develop clinical listeriosis. neutrophils are innate immune cells required for the clearance of pathogenic organisms, including l. monocytog ... | 2016 | 27600509 |
| pyelonephritis with bacteremia caused by listeria monocytogenes: a case report. | listeria monocytogenes is a well-known cause of meningitis, colitis, and bacteremia; however, obstructive pyelonephritis caused by l. monocytogenes has never been reported. we herein report on a 90-year-old japanese woman with obstructive pyelonephritis and bacteremia due to uterus carcinoma invading the ureter. she was admitted to our hospital complaining of fever and chills, and her physical examination revealed left costovertebral angle tenderness. computed tomography showed hydronephrosis an ... | 2017 | 27600175 |
| chemistry and molecular dynamics simulations of heme b-hemq and coproheme-hemq. | recently, a novel pathway for heme b biosynthesis in gram-positive bacteria has been proposed. the final poorly understood step is catalyzed by an enzyme called hemq and includes two decarboxylation reactions leading from coproheme to heme b. coproheme has been suggested to act as both substrate and redox active cofactor in this reaction. in the study presented here, we focus on hemqs from listeria monocytogenes (lmhemq) and staphylococcus aureus (sahemq) recombinantly produced as apoproteins in ... | 2016 | 27599156 |
| molecular characterization and phylogenetic analysis of listeria monocytogenes isolated from milk and milk products in kaduna, nigeria. | in this study, listeria (l.) monocytogenes isolated from milk and milk products in kaduna, nigeria, were subjected to a multiplex pcr assay to identify virulence-associated genes (such as prf a, inl a, hly a, act a, and iap). of the 36 isolates, 9 (25%) were positive for one or two virulence-associated genes. based on the sample type, 6 (16.9%) of the isolates that possessed virulence-associated genes were obtained from raw milk, 2 (3.2%) from "manshanu," and 1 (2.8%) from "kindrimo." sequence a ... | 2016 | 27597873 |
| the diverse family of arp2/3 complexes. | the arp2/3 complex has so far been considered to be a single seven-subunit protein complex required for actin nucleation and actin filament polymerization in diverse critical cellular functions including phagocytosis, vesicular trafficking and lamellipodia extension. the arp2/3 complex is also exploited by bacterial pathogens and viruses during cellular infectious processes. recent studies suggest that some subunits of the complex are dispensable in specific cellular contexts, pointing to the ex ... | 2017 | 27595492 |
| characterization of a plasmid carrying cat, ermb and tets genes in a foodborne listeria monocytogenes strain and uptake of the plasmid by cariogenic streptococcus mutans. | a multi-drug resistant (mdr) listeria monocytogenes isolate (serotype 1/2c) was recovered from a quick-frozen rice flour product collected from langfang city in northern china. pcr screening identified the presence of cat, ermb and tets genes. the plasmid profile of the strain showed the presence of an approximately 22.4-kb plasmid. curing of this plasmid resulted in the loss of cat, ermb and tets genes and increased susceptibility to several antibiotics, suggesting the involvement of the plasmi ... | 2016 | 27592072 |
| inhibition effect of tea tree oil on listeria monocytogenes growth and exotoxin proteins listeriolysin o and p60 secretion. | listeria monocytogenes (l. monocytogenes) is a gram-positive bacterium that causes infections in humans. in this study, the effects of tea tree oil (tto) at subinhibitory concentrations on l. monocytogenes growth and two important exotoxin proteins secreted by l. monocytogenes were researched. treatment with half of minimal inhibitory concentration of tto demonstrated very little or no reduction in numbers of viable atcc 19115 cells. listeriolysin o (llo) and p60, were investigated. a listerioly ... | 2016 | 27591558 |
| survival of foodborne pathogens (escherichia coli o157:h7, salmonella typhimurium, staphylococcus aureus, listeria monocytogenes, and vibrio parahaemolyticus) in raw ready-to-eat crab marinated in soy sauce. | knowing the survival characteristics of foodborne pathogens in raw ready-to-eat (rte) seafood is the key to predicting whether they pose a microbiological hazard. the present study examined the survival of escherichia coli o157:h7, salmonella typhimurium, vibrio parahaemoliticus, listeria monocytogenes, and staphylococcus aureus in raw rte crab marinated in soy sauce. inoculated crabs (initial bacterial population=4.1-4.4logcfu/g) were immersed in soy sauce and then stored at refrigeration (5°c) ... | 2016 | 27591386 |
| in vitro activity of plant extracts against biofilm-producing food-related bacteria. | the identification of effective antimicrobial agents also active on biofilms is a topic of crucial importance in food and industrial environment. for that purpose methanol extracts of turkish plants, ficus carica l., juglans regia l., olea europaea l., punica granatum l. and rhus coriaria l., were investigated. among the extracts, p. granatum l. and r. coriaria l. showed the best antibacterial activity with minimum inhibitory concentrations (mic) of 78-625μg/ml for listeria monocytogenes and sta ... | 2016 | 27591384 |
| neonatal listeriosis: uncommon or misdiagnosed? | the incidence of perinatal and neonatal listeriosis is underestimated due undiagnosed stillbirths, misdiagnosis of nl and underreporting of single case reports. recent outbreaks reinforce the need for better surveillance and targeted health education in certain population groups especially during pregnancy. | 2016 | 27589546 |
| development of synthetic media mimicking food soils to study the behaviour of listeria monocytogenes on stainless steel surfaces. | listeria monocytogenes is one of the main targets of hygiene procedures in the ready-to-eat food industry due to its ability to persist for months or even years in processing plants, where it can contaminate food during processing. the factors associated with persistence are often those that foster growth, which itself depends on food contamination of surfaces. it is therefore essential to experiment by using food soils or media modelling these soils to understand the behaviour of l. monocytogen ... | 2016 | 27589019 |
| epidermal fatty acid binding protein (e-fabp) is not required for the generation or maintenance of effector and memory t cells following infection with listeria monocytogenes. | following activation of naïve t cells there are dynamic changes in the metabolic pathways used by t cells to support both the energetic needs of the cell and the macromolecules required for growth and proliferation. among other changes, lipid metabolism undergoes dynamic transitions between fatty acid oxidation and fatty acid synthesis as cells progress from naïve to effector and effector to memory t cells. the hydrophobic nature of lipids requires that they be bound to protein chaperones within ... | 2016 | 27588422 |
| outbreak of listeria monocytogenes infections linked to a pasteurized ice cream product served to hospitalized patients. | two cases of hospital-acquired listeriosis were linked to a commercially produced, pasteurized ice cream mix. manufacturers should implement safety measures from the food safety modernization act to minimize the risk of listeria contamination. dietary guidelines for persons at high risk of listeriosis may need revision to recognize the potential risk from pasteurized products. | 2016 | 27586030 |
| decay rates of zoonotic pathogens and viral surrogates in soils amended with biosolids and manures and comparison of qpcr and culture derived rates. | the purpose of this study was to establish inactivation decay constants of foodborne pathogens and coliphage in clay and sandy soils for future "downstream" analyses such as quantitative microbial risk analysis and to compare cultivation-dependent and -independent (e.g. qpcr) methods. | 2016 | 27585434 |
| factors contributes to spontaneous abortion caused by listeria monocytogenes, in tehran, iran, 2015. | spontaneous abortion is the loss of a fetus before the 20th week of pregnancy, when occurring naturally without any surgical or pharmaceutical intervention. on the other hand, listeria monocytogenes, as one of the foodborne pathogens, is a causative agent of listeriosis. the transfer of l. monocytogenes in pregnant women occurs as self-limited flu-like symptoms which may result in abortion, stillbirth or premature birth of infected infants. the purpose of this study was the identification of lis ... | 2016 | 27585255 |
| variability of listeria monocytogenes strains in biofilm formation on stainless steel and polystyrene materials and resistance to peracetic acid and quaternary ammonium compounds. | listeria monocytogenes is a foodborne pathogen able to tolerate adverse conditions by forming biofilms or by deploying stress resistant mechanisms, and thus manages to survive for long periods in food processing plants. this study sought to investigate the correlation between biofilm forming ability, tolerance to disinfectants and cell surface characteristics of twelve l. monocytogenes strains. the following attributes were evaluated: (i) biofilm formation by crystal violet staining method on po ... | 2016 | 27585076 |
| type i interferon promotes cell-to-cell spread of listeria monocytogenes. | type i interferons (ifns) play a critical role in antiviral immune responses, but can be deleterious to the host during some bacterial infections. listeria monocytogenes (lm) induces a type i ifn response by activating cytosolic antiviral surveillance pathways. this is beneficial to the bacteria as mice lacking the type i ifn receptor (ifnar1(-/-) ) are resistant to systemic infection by lm. the mechanisms by which type i ifns promote lm infection are unclear. here, we show that ifnar1 is requir ... | 2017 | 27582004 |
| purification of a novel bacteriocin-like inhibitory substance produced by enterococcus faecium icis 8 and characterization of its mode of action. | the aim of this work was to purify and characterize a bacteriocin-like antimicrobial substance produced by an antagonistic active strain of enterococcus faecium. | 2016 | 27575914 |
| spontaneous symmetry breaking for geometrical trajectories of actin-based motility in three dimensions. | actin-based motility is important for many cellular processes. in this article we extend our previous studies of an actin-propelled circular disk in two dimensions to an actin-propelled spherical bead in three dimensions. we find that for an achiral load the couplings between the motion of the load and the actin network induce a series of bifurcations, starting with a transition from rest to moving state, followed by a transition from straight to planar curves, and finally a further transition f ... | 2016 | 27575158 |
| core-shell nanorod columnar array combined with gold nanoplate-nanosphere assemblies enable powerful in situ sers detection of bacteria. | development of a label-free ultrasensitive nanosensor for detection of bacteria is presented. sensitive assay for gram-positive bacteria was achieved via electrostatic attraction-guided plasmonic bifacial superstructure/bacteria/columnar array assembled in one step. dynamic optical hotspots were formed in the hybridized nanoassembly under wet-dry critical state amplifying efficiently the weak vibrational modes of three representative food-borne gram-positive bacteria, that is, staphylococcus xyl ... | 2016 | 27574829 |
| oral exposure to listeria monocytogenes in aged il-17rko mice: a possible murine model to study listeriosis in susceptible populations. | foodborne listeria monocytogenes (lm) is a cause of serious illness and death in the us. the case-fatality rate of invasive lm infection in the elderly population is >50%. the goal of this study is to establish a murine model of oral lm infection that can be used as a surrogate for human foodborne listeriosis in the geriatric population. adult c57bl/6 (wild-type, wt) and adult or old il17r-ko (knock-out) mice were gavaged with a murinized lm strain (lmo-inla(m)) and monitored for body-weight los ... | 2016 | 27574777 |
| c-met inhibitors are potential novel therapeutic agents against listeria monocytogenes infection through blocking the bacteria entry into nonphagocytic cells. | high lethality of infections caused by listeria monocytogenes still remains a major clinical problem in spite of their susceptibility to a wide spectrum of antibiotics. the refractoriness towards treatment is primarily due to its amazing capacity to invade non-phagocytic cells and replicate there in, imparting the dual protection from immune response and antimicrobials. therefore, generating new anti-infective drugs against intracellular infections has emerged as an urgent issue in the therapeut ... | 2017 | 27572078 |
| anti-listeria effects of chitosan-coated nisin-silica liposome on cheddar cheese. | listeria monocytogenes poses an increasing challenge to cheese production. to minimize the risk of bacterial contamination, a chitosan-coated nisin-silica liposome was engineered for the present study. we investigated the characteristics of nisin-silica liposomes and the anti-listeria effects of a chitosan-coated nisin-silica liposome on cheddar cheese. the encapsulation efficiency of nisin in a liposome was sharply increased after it was adsorbed on a silica particle surface. chitosan-coated ni ... | 2016 | 27568051 |
| dielectric barrier discharge atmospheric cold plasma inhibits escherichia coli o157:h7, salmonella, listeria monocytogenes, and tulane virus in romaine lettuce. | the present study investigated the effects of dielectric barrier discharge atmospheric cold plasma (dacp) treatment on the inactivation of escherichia coli o157:h7, salmonella, listeria monocytogenes, and tulane virus (tv) on romaine lettuce, assessing the influences of moisture vaporization, modified atmospheric packaging (map), and post-treatment storage on the inactivation of these pathogens. romaine lettuce was inoculated with e. coli o157:h7, salmonella, l. monocytogenes (~6logcfu/g lettuce ... | 2016 | 27562348 |
| notes from the field: outbreak of listeriosis associated with consumption of packaged salad - united states and canada, 2015-2016. | in september 2015, pulsenet, the national molecular subtyping network for foodborne disease surveillance, identified a cluster of listeria monocytogenes (listeria) clinical isolates indistinguishable by two-enzyme pulsed-field gel electrophoresis (pfge) pattern combination and highly related by whole-genome multilocus sequence typing (wgmlst). a case was defined as isolation of listeria with the outbreak pfge pattern and highly related by wgmlst with an isolation date on or after july 5, 2015, t ... | 2016 | 27559935 |
| serial magnetic resonance imaging findings of intracerebral spread of listeria utilising subcortical u-fibres and the extreme capsule. | we present a case of listeria monocytogenes cerebral abscess with axonal spread via the subcortical u-fibres and extreme capsule on magnetic resonance imaging, with follow-up studies demonstrating serial reduction in oedema and enhancement pattern of the white-matter fibre tracts following antimicrobial treatment. we discuss the microbiological mechanism of bacterial mobility to account for these unique imaging features. recognition of this distinct pattern of spread of l. monocytogenes cerebral ... | 2016 | 27558992 |
| a one health overview, facilitating advances in comparative medicine and translational research. | a1 one health advances and successes in comparative medicine and translational researchcheryl strouda2 dendritic cell-targeted gorilla adenoviral vector for cancer vaccination for canine melanomaigor dmitriev, elena kashentseva, jeffrey n. bryan, david t. curiela3 viroimmunotherapy for malignant melanoma in the companion dog modeljeffrey n. bryan, david curiel, igor dmitriev, elena kashentseva, hans rindt, carol reinero, carolyn j. henrya4 of mice and men (and dogs!): development of a commercial ... | 0 | 27558513 |
| evaluation of the performance of quantitative detection of the listeria monocytogenes prfa locus with droplet digital pcr. | fast and reliable pathogen detection is an important issue for human health. since conventional microbiological methods are rather slow, there is growing interest in detection and quantification using molecular methods. the droplet digital polymerase chain reaction (ddpcr) is a relatively new pcr method for absolute and accurate quantification without external standards. using the listeria monocytogenes specific prfa assay, we focused on the questions of whether the assay was directly transferab ... | 2016 | 27558101 |
| modeling the behavior of listeria monocytogenes during enrichment in half fraser broth; impact of pooling and the duration of enrichment on the detection of l. monocytogenes in food. | a stochastic model describing the growth of listeria monocytogenes during enrichment in half fraser was developed for the purpose of estimating the effects of modifications to the first enrichment step of the en iso 11290-1 detection method. information pertaining to the variability of growth rates, physiological state of the cell, and the behavior of individual cells contaminating the food were obtained from previously published studies. we used this model to investigate the impact of pooling e ... | 2016 | 27554154 |
| influence of water activity on inactivation of escherichia coli o157:h7, salmonella typhimurium and listeria monocytogenes in peanut butter by microwave heating. | this study evaluated the efficacy of a 915 mhz microwave with 3 different electric power levels to inactivate three pathogens in peanut butter with different aw. peanut butter inoculated with escherichia coli o157:h7, salmonella enterica serovar typhimurium and listeria monocytogenes (0.3, 0.4, and 0.5 aw) were treated with a 915 mhz microwave with 2, 4, and 6 kw for up to 5 min. six kw 915 mhz microwave treatment for 5 min reduced these three pathogens by 1.97 to >5.17 log cfu/g. four kw 915 mh ... | 2016 | 27554151 |
| do bacterial cell numbers follow a theoretical poisson distribution? comparison of experimentally obtained numbers of single cells with random number generation via computer simulation. | we investigated a bacterial sample preparation procedure for single-cell studies. in the present study, we examined whether single bacterial cells obtained via 10-fold dilution followed a theoretical poisson distribution. four serotypes of salmonella enterica, three serotypes of enterohaemorrhagic escherichia coli and one serotype of listeria monocytogenes were used as sample bacteria. an inoculum of each serotype was prepared via a 10-fold dilution series to obtain bacterial cell counts with me ... | 2016 | 27554145 |
| modeling optimal process conditions for uv-heat inactivation of foodborne pathogens in liquid foods. | the combination of ultraviolet radiation and heat (uv-h treatment) has been demonstrated as a promising strategy to overcome the limited uv germicidal effect in fruit juices. nonetheless, there are so far no data regarding the efficacy of the combined process for the inactivation of bacterial foodborne pathogens in other liquid foods with different ph and composition. in this investigation, the optimum uv-h processing conditions for the inactivation of escherichia coli, salmonella typhimurium, l ... | 2016 | 27554141 |
| transport and catabolism of pentitols by listeria monocytogenes. | transposon insertion into listeria monocytogenes lmo2665, which encodes an eiic of the phosphoenolpyruvate:carbohydrate phosphotransferase system (pts), was found to prevent d-arabitol utilization. we confirm this result with a deletion mutant and show that lmo2665 is also required for d-xylitol utilization. we therefore called this protein eiicaxl. both pentitols are probably catabolized via the pentose phosphate pathway (ppp) because lmo2665 belongs to an operon, which encodes the three ptsaxl ... | 2016 | 27553222 |
| pregnane x receptor regulates pathogen-induced inflammation and host defense against an intracellular bacterial infection through toll-like receptor 4. | the nuclear pregnane x receptor (pxr) plays a central role in regulating xenobiotic metabolism. we now report a novel role for pxr as a critical negative regulator of innate immunity after infection. pxr(-/-) mice exhibited remarkably elevated pro-inflammatory cytokine and chemokine production following infection with listeria monocytogenes (lm). despite the more robust innate immune response, pxr(-/-) mice were highly susceptible to lm infection. surprisingly, disruption of the toll-like recept ... | 2016 | 27550658 |
| survival and growth of listeria monocytogenes on fresh-cut "athena" and "rocky ford" cantaloupes during storage at 4°c and 10°c. | cantaloupes, marketed as "rocky ford," were implicated in the u.s. multistate outbreak of listeriosis in 2011, which caused multiple fatalities. listeria monocytogenes can survive on whole cantaloupes and can be transferred to the flesh of melons. the growth of l. monocytogenes on fresh-cut "athena" and "rocky ford" cantaloupe cultivars during refrigerated storage was evaluated. fresh-cut cubes (16.4 cm(3)) from field-grown cantaloupes were each inoculated with 5 log10 cfu/ml of a multi-strain m ... | 2016 | 27548768 |
| rapid colorimetric sensing platform for the detection of listeria monocytogenes foodborne pathogen. | listeria monocytogenes is a serious cause of human foodborne infections worldwide, which needs spending billions of dollars for inspection of bacterial contamination in food every year. therefore, there is an urgent need for rapid, in-field and cost effective detection techniques. in this study, rapid, low-cost and simple colorimetric assay was developed using magnetic nanoparticles for the detection of listeria bacteria. the protease from the listeria bacteria was detected using d-amino acid su ... | 2016 | 27543841 |
| morin inhibits biofilm production and reduces the virulence of listeria monocytogenes - an in vitro and in vivo approach. | the current study explores the in vitro and in vivo antibiofilm efficacy of morin against a leading foodborne pathogen-listeria monocytogenes (lm). minimum inhibitory concentration (mic) of morin against lm strains was found to be 100μg/ml. the non-antibacterial effect of morin at its sub-mics (6.25, 12.5 and 25μg/ml) was determined through growth curve and xtt assay. morin at its sub-mics demonstrated a significant dose dependent inhibitory efficacy against lm biofilm formation which was also e ... | 2016 | 27543817 |
| the four-component aureocin a70 as a promising agent for food biopreservation. | aureocin a70 is the only four-component bacteriocin described to date. as it inhibits the growth of a wide range of gram-positive bacteria, including listeria monocytogenes strains isolated from food, its potential for improving food safety was investigated in this study. aureocin a70 (10,240au/ml) proved to be bactericidal, but not extensively lytic, against listerial strains. the antibacterial activity of aureocin a70 (16au/ml) was then tested in uht-treated skimmed milk inoculated with the fo ... | 2016 | 27543814 |
| the essential role of neutrophils during infection with the intracellular bacterial pathogen listeria monocytogenes. | neutrophils have historically been characterized as first responder cells vital to host survival because of their ability to contain and eliminate bacterial and fungal pathogens. however, recent studies have shown that neutrophils participate in both protective and detrimental responses to a diverse array of inflammatory and infectious diseases. although the contribution of neutrophils to extracellular infections has been investigated for decades, their specific role during intracellular bacteri ... | 2016 | 27543669 |