Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| key role of teichoic acids on aflatoxin b binding by probiotic bacteria. | to assess the ability of five probiotic bacteria to bind aflatoxin b(1) and to determine the key role of teichoic acids in the binding mechanism. | 2009 | 19486416 |
| oral colonization by lactobacillus reuteri atcc 55730 after exposure to probiotics. | the aim of this study was to investigate whether lactobacillus reuteri atcc 55730 can be detected in the oral cavity after discontinuation of administration of a product prepared with this bacterium. | 2009 | 19486373 |
| identification and characterization of antibiotic resistance genes in lactobacillus reuteri and lactobacillus plantarum. | the study aimed to identify the resistance genes mediating atypical minimum inhibitory concentrations (mics) for tetracycline, erythromycin, clindamycin and chloramphenicol within two sets of representative strains of the species lactobacillus reuteri and lactobacillus plantarum and to characterize identified genes by means of gene location and sequencing of flanking regions. | 2009 | 19457037 |
| identification and evaluation of the probiotic potential of lactobacilli isolated from canine milk. | canine milk protects puppies against infectious diseases through a variety of mechanisms. in this study, the presence of potentially probiotic lactic acid bacteria grown on mrs-cys agar plates from milk of nine bitches was investigated. the gram positive catalase negative bacilli identified in this study belonged to four lactobacillus species (lactobacillus reuteri, lactobacillus fermentum, lactobacillus murinus, lactobacillus animalis), as well as one isolate that was identified as weissella vi ... | 2010 | 19451012 |
| effect of amino acid availability on vitamin b12 production in lactobacillus reuteri. | recent functional genomics and genome-scale modeling approaches indicated that b(12) production in lactobacillus reuteri could be improved by optimization of the medium. here we show that a series of systematic single-amino-acid omissions could significantly modulate the production of b(12) from nearly undetectable levels (with omission of isoleucine) to levels 20-fold higher than the levels previously reported (with omission of cysteine). using cdna microarray experiments, we analyzed the trans ... | 2009 | 19376900 |
| volatile sulfur compounds produced by probiotic bacteria in the presence of cysteine or methionine. | to investigate the abilities of various probiotic bacteria to produce volatile sulfur compounds (vscs) relevant to food flavour and aroma. | 2009 | 19344359 |
| a t7 rna polymerase-dependent gene expression system for bacillus megaterium. | gene expression systems based on the rna polymerase of the bacteriophage t7 are often the ultimate choice for the high level production of recombinant proteins. during the last decade, the gram-positive bacterium bacillus megaterium was established as a useful host for the intra- and extracellular production of heterologous proteins. in this paper, we report on the development of a t7 rna polymerase-dependent expression system for b. megaterium. the system was evaluated for cytosolic and secreto ... | 2009 | 19308404 |
| improved cure of bacterial vaginosis with single dose of tinidazole (2 g), lactobacillus rhamnosus gr-1, and lactobacillus reuteri rc-14: a randomized, double-blind, placebo-controlled trial. | bacterial vaginosis (bv) is the most prevalent vaginal infection worldwide and is characterized by depletion of the indigenous lactobacilli. antimicrobial therapy is often ineffective. we hypothesized that probiotic lactobacillus rhamnosus gr-1 and lactobacillus reuteri rc-14 might provide an adjunct to antimicrobial treatment and improve cure rates. sixty-four brazilian women diagnosed with bv were randomly assigned to receive a single dose of tinidazole (2 g) supplemented with either 2 placebo ... | 2009 | 19295645 |
| residue phe112 of the human-type corrinoid adenosyltransferase (pduo) enzyme of lactobacillus reuteri is critical to the formation of the four-coordinate co(ii) corrinoid substrate and to the activity of the enzyme. | atp:corrinoid adenosyltransferases (acas) catalyze the transfer of the adenosyl moiety from atp to cob(i)alamin via a four-coordinate cob(ii)alamin intermediate. at present, it is unknown how acas promote the formation of the four-coordinate corrinoid species needed for activity. the published high-resolution crystal structure of the aca from lactobacillus reuteri (lrpduo) in complex with atp and cob(ii)alamin shows that the environment around the alpha face of the corrin ring consists of bulky ... | 2009 | 19236001 |
| probiotic lactobacillus reuteri biofilms produce antimicrobial and anti-inflammatory factors. | commensal-derived probiotic bacteria inhibit enteric pathogens and regulate host immune responses in the gastrointestinal tract, but studies examining specific functions of beneficial microbes in the context of biofilms have been limited in scope. | 2009 | 19210794 |
| lactobacillus reuteri enhances excitability of colonic ah neurons by inhibiting calcium-dependent potassium channel opening. | probiotics are live non-pathogenic commensal organisms that exert therapeutic effects in travellers' diarrhea, irritable bowel syndrome and inflammatory bowel disease. little is known about mechanisms of action of commensal bacteria on intestinal motility and motility-induced pain. it has been proposed that probiotics affect intestinal nerve function, but direct evidence for this has thus far been lacking. we hypothesized that probiotic effects might be mediated by actions on colonic intrinsic s ... | 2009 | 19210574 |
| lactobacillus reuteri crl 1098 prevents side effects produced by a nutritional vitamin b deficiency. | to evaluate the efficiency of the vitamin b(12-)producing lactobacillus reuteri crl1098 strain in preventing the symptoms caused by a nutritional cobalamin-deficient diet in pregnant female mice and their weaned offspring. | 2009 | 19200314 |
| improved treatment of vulvovaginal candidiasis with fluconazole plus probiotic lactobacillus rhamnosus gr-1 and lactobacillus reuteri rc-14. | to determine the ability of probiotic lactobacilli to improve the treatment of vulvovaginal candidiasis (vvc) using a randomized, double-blind and placebo-controlled trial. | 2009 | 19187507 |
| structural characterization of bioengineered alpha-d-glucans produced by mutant glucansucrase gtf180 enzymes of lactobacillus reuteri strain 180. | mutagenesis of specific amino acid residues of the glucansucrase (gtf180) enzyme from lactobacillus reuteri strain 180 yielded 12 mutant enzymes that produced modified exopolysaccharides (mepss) from sucrose. ethanol-precipitated and purified mepss were subjected to linkage analysis, smith degradation analysis, and 1d/2d (1)h nmr spectroscopy. comparison of the results with structural data of the previously described wild type eps180 and triple mutant meps-pnns revealed a broad variation of stru ... | 2009 | 19186951 |
| lactobacillus reuteri ingestion prevents hyperexcitability of colonic drg neurons induced by noxious stimuli. | lactobacillus species ingestion can decrease autonomic responses and spinal fiber discharge to nociceptive colorectal distension (crd), even in the absence of inflammation. the present study aimed to determine whether dorsal root ganglion (drg) somas could be a locus where the antinociceptive probiotic may have an effect. healthy rats were fed with lactobacillus reuteri or vehicle control for 9 days whereupon they were anesthetized, and intermittent distal colonic crd at 80 mmhg distension was e ... | 2009 | 19179624 |
| lactobacillus reuteri prevents colitis by reducing p-selectin-associated leukocyte- and platelet-endothelial cell interactions. | recent findings indicate that dextran sodium sulfate (dss)-induced colitis is associated with a prothrombogenic phenotype, with p-selectin playing a major role in platelet recruitment. it has been suggested that probiotics may ameliorate colonic inflammation. we therefore investigated how treatment with lactobacillus reuteri influenced p-selectin expression, leukocyte and platelet endothelial cell interactions, and colitis severity in dss-treated rats. rats were divided into the following four g ... | 2009 | 19147805 |
| cystathionine gamma-lyase is a component of cystine-mediated oxidative defense in lactobacillus reuteri br11. | lactobacillus reuteri br11 possesses a novel mechanism of oxidative defense involving an abundant cystine abc transporter encoded by the cyuabc gene cluster. large amounts of thiols, including h(2)s, are secreted upon cystine uptake by the cyuc transporter. a cystathionine gamma-lyase (cgl) gene is cotranscribed with the cyu genes in several l. reuteri strains and was hypothesized to participate in cystine-mediated oxidative defense by producing reducing equivalents. this hypothesis was tested w ... | 2009 | 19124577 |
| change of intestinal microbiota with elemental diet and its impact on therapeutic effects in a murine model of chronic colitis. | elemental diet (ed) has been used as an enteral nutritional therapy for crohn's disease. however, the precise mechanisms of ed remain unclear. in interleukin-10 (il-10)-deficient cell-transferred mice, we investigated the change of intestinal microbiota with ed using molecular terminal-restriction fragment length polymorphism (t-rflp) analysis and culture method, and evaluated its influence on therapeutic effects of ed. ed significantly suppressed intestinal inflammation. the total amount of bac ... | 2009 | 19058004 |
| toll-like receptor and innate cytokine responses induced by lactobacilli colonization and human rotavirus infection in gnotobiotic pigs. | toll-like receptors (tlr) play an important role in the recognition of microbes by host sentinel cells that leads to the subsequent innate and adaptive immune responses. in this study, we evaluated the patterns of tlr2-, tlr3- and tlr9-expressing antigen presenting cells (apcs) in spleen and blood of gnotobiotic (gn) pigs after colonization with a mixture of two strains of lactic acid bacteria (lab), lactobacillus acidophilus and lactobacillus reuteri or infection with the virulent human rotavir ... | 2009 | 19054578 |
| lactobacillus reuteri-induced regulatory t cells protect against an allergic airway response in mice. | we have previously demonstrated that oral treatment with live lactobacillus reuteri can attenuate major characteristics of the asthmatic response in a mouse model of allergic airway inflammation. however, the mechanisms underlying these effects remain to be determined. | 2009 | 19029003 |
| hybrid reuteransucrase enzymes reveal regions important for glucosidic linkage specificity and the transglucosylation/hydrolysis ratio. | the reuteransucrase enzymes of lactobacillus reuteri strain 121 (gtfa) and l. reuteri strain atcc 55730 (gtfo) convert sucrose into alpha-d-glucans (labelled reuterans) with mainly alpha-(1-->4) glucosidic linkages (50% and 70%, respectively), plus alpha-(1-->6) linkages. in the present study, we report a detailed analysis of various hybrid gtfa/o enzymes, resulting in the identification of specific regions in the n-termini of the catalytic domains of these proteins as the main determinants of g ... | 2008 | 19016850 |
| short-term effect of chewing gums containing probiotic lactobacillus reuteri on the levels of inflammatory mediators in gingival crevicular fluid. | to investigate the effect of a chewing gum containing probiotic bacteria on gingival inflammation and the levels of selected inflammatory mediators in gingival crevicular fluid (gcf). | 2009 | 18985460 |
| sugar fermentation in probiotic bacteria--an in vitro study. | food supplemented with probiotic bacteria is a rapidly growing sector of the market. the aim of the present study was to evaluate and compare the acid production of selected probiotic strains available in commercial products. | 2008 | 18954354 |
| [efficacy of lactobacillus rhamnosus gr-1 and of lactobacillus reuteri rc-14 in the treatment and prevention of vaginoses and bacterial vaginitis relapses]. | the aim of this study was to investigate the efficacy of the use of lactobacillus rhamnosus gr-1 and of lactobacillus reuteri rc-14 administrated orally in the treatment and prevention of vaginoses and bacterial vaginitis relapses. | 2008 | 18854803 |
| assessment of dietary fiber fermentation: effect of lactobacillus reuteri and reproducibility of short-chain fatty acid concentrations. | this investigation had two aims: (i) to determine the reproducibility of scfa production of two fibers: wheat dextrin and inulin, in two separate in vitro batch fermentation systems, and (ii) to determine if the addition lactobacillus reuteri, a probiotic bacterium, enhanced the fermentation of wheat dextrin, inulin, and psyllium using in vitro batch fermentation. samples were removed at 0, 4, 8, 12, and 24 h. scfas were measured by gc. l. reuteri improved inulin's fermentation profile by reduci ... | 2009 | 18837468 |
| isolation and characterization of potential probiotic lactobacilli from pig feces. | this study examined four lactobacilli isolated from pig feces. two hundred lactic acid bacteria strains were obtained from pig feces using selective culture media (with vancomycin and bromocresol green; termed lamvab agar). microscopy, the catalase test, gram-staining, and rapd-pcr analysis were used to group the bacteria into 20 related clusters. phenotypic analysis using the api 50 ch test and genotypic analysis of 16s rdna sequences identified these clusters as representing single strains of ... | 2009 | 18792047 |
| global transcriptional response of lactobacillus reuteri to the sourdough environment. | lactobacillus reuteri is a lactic acid bacterium that is highly adapted to the sourdough environment. it is a dominant member of industrial type ii sourdoughs, and is also able to colonize the intestinal tract of mammals, including humans, and birds. in this study, the transcriptional response of l. reuteri atcc 55730 was investigated during sourdough fermentation by using whole-genome microarrays. significant changes of mrna levels were found for 101 genes involved in diverse cellular processes ... | 2008 | 18762399 |
| adherence of clinically isolated lactobacilli to human cervical cells in competition with neisseria gonorrhoeae. | lactobacilli are normal inhabitants of our microbiota and are known to protect against pathogens. neisseria gonorrhoeae is a human specific pathogenic bacterium that colonises the urogenital tract where it causes gonorrhoea. in this study we analysed early interactions between lactobacilli and gonococci and investigated how they compete for adherence to human epithelial cervical cells. we show that lactobacilli adhere at various levels and that the number of adherent bacteria does not correlate ... | 2008 | 18761100 |
| triple therapies plus different probiotics for helicobacter pylori eradication. | the helicobacter pylori (h. pylori) cure rate following standard triple therapies is decreasing worldwide. therefore, further approaches aimed to improve standard triple therapy efficacy should be attempted. this prospective, pilot study aimed to evaluate the therapeutic role of either lactobacillus reuteri (l. reuteri) or a high concentration of probiotics in addition to standard triple therapies for h. pylori eradication. the study enrolled 65 consecutive dyspeptic patients with h. pylori infe ... | 2008 | 18727457 |
| microencapsulated bile salt hydrolase producing lactobacillus reuteri for oral targeted delivery in the gastrointestinal tract. | this is the first study of its kind to screen probiotic lactic acid bacteria for the purpose of microencapsulating a highly bile salt hydrolase (bsh)-active strain. a lactobacillus reuteri strain and a bifidobacterium longum strain were isolated as the highest bsh producers among the candidates. microcapsules were prepared with a diameter of 619 +/- 31 mum and a cell load of 5 x 10(9) cfu/ml. post de man, rogosa, and sharpe broth-acid challenge, l. reuteri microcapsules metabolized glyco- and ta ... | 2008 | 18719901 |
| lactobacillus reuteri 100-23 transiently activates intestinal epithelial cells of mice that have a complex microbiota during early stages of colonization. | monoassociations of germ-free animals with colitogenic and probiotic bacterial strains trigger intestinal epithelial cell (iec) activation and host-derived feedback mechanisms. to characterize the impact of a single nonpathogenic bacterial strain on the intestinal epithelium in the presence of an established microbiota, we inoculated reconstituted lacotobacillus-free (rlf) mice at 8 wk of age with lactobacillus reuteri 100-23. primary iec from the small intestine of l. reuteri-inoculated and con ... | 2008 | 18716170 |
| estimation of 16s rrna gene copy number in several probiotic lactobacillus strains isolated from the gastrointestinal tract of chicken. | the copy numbers of 16s rrna genes in 12 probiotic lactobacillus strains of poultry origin were analyzed. genomic dna of the strains was digested with restriction endonucleases that do not cut within the 16s rrna gene of the strains. this was followed by southern hybridization with a biotinylated probe complementary to the 16s rrna gene. the copy number of the 16s rrna gene within a lactobacillus species was found to be conserved. from the hybridization results, lactobacillus salivarius i 24 was ... | 2008 | 18707622 |
| randomized, double-blind, placebo-controlled study of oral lactobacilli to improve the vaginal flora of postmenopausal women. | the purpose of this randomized, double-blind, placebo-controlled study was to evaluate the influence of the orally administered probiotic strains lactobacillus rhamnosus gr-1 and lactobacillus reuteri rc-14 on the quality of the vaginal flora in postmenopausal women. | 2008 | 18701205 |
| removal of antibiotic resistance gene-carrying plasmids from lactobacillus reuteri atcc 55730 and characterization of the resulting daughter strain, l. reuteri dsm 17938. | the spread of antibiotic resistance in pathogens is primarily a consequence of the indiscriminate use of antibiotics, but there is concern that food-borne lactic acid bacteria may act as reservoirs of antibiotic resistance genes when distributed in large doses to the gastrointestinal tract. lactobacillus reuteri atcc 55730 is a commercially available probiotic strain which has been found to harbor potentially transferable resistance genes. the aims of this study were to define the location and n ... | 2008 | 18689509 |
| probiotic lactobacilli for urogenital health in women. | the microbiota of the vagina form mostly from ascension of microbes from the rectal area. the numbers and types of microbes fluctuates with hormone levels, sexual contact, douching, and diet, yet the basic composition is relatively simple, with lactobacilli dominant in healthy females. the depletion of these organisms in women susceptible to urinary and vaginal infections, raised the question of whether artificial supplementation of lactobacilli could lower infection rates. to date, a 2 strain c ... | 2008 | 18685506 |
| proteomic analysis of the effect of bile salts on the intestinal and probiotic bacterium lactobacillus reuteri. | lactobacillus reuteri is a resident of the human and animal intestinal tracts. the ability of l. reuteri to survive passage through the intestinal tract is a key point in its function as a probiotic. in order to examine the nature of bile salt tolerance by l. reuteri, its protein synthesis was analyzed in liquid cultures containing two different bile salt conditions. significant cell growth inhibition was observed in the presence of 1.2g/l (higher concentration) bile salts. two-dimensional gel e ... | 2008 | 18680767 |
| kinetic and spectroscopic studies of the atp:corrinoid adenosyltransferase pduo from lactobacillus reuteri: substrate specificity and insights into the mechanism of co(ii)corrinoid reduction. | the pduo-type atp:corrinoid adenosyltransferase from lactobacillus reuteri ( lrpduo) catalyzes the formation of the essential co-c bond of adenosylcobalamin (coenzyme b 12) by transferring the adenosyl group from cosubstrate atp to a transient co (1+)corrinoid species generated in the enzyme active site. while pduo-type enzymes have previously been believed to be capable of adenosylating only co (1+)cobalamin (co (1+)cbl (-)), our kinetic data obtained in this study provide in vitro evidence tha ... | 2008 | 18672897 |
| structural analysis of bioengineered alpha-d-glucan produced by a triple mutant of the glucansucrase gtf180 enzyme from lactobacillus reuteri strain 180: generation of (alpha1-->4) linkages in a native (1-->3)(1-->6)-alpha-d-glucan. | site-directed mutagenesis of the glucansucrase gtf180 gene from lactobacillus reuteri strain 180 was used to transform the active site region. the alpha-d-glucan ( meps-pnns) produced by the triple mutant v1027p:s1137n:a1139s differed in structure from that of the wild-type alpha-d-glucan ( eps180). besides (alpha1-->3) and (alpha1-->6) linkages, as present in eps180, meps-pnns also contained (alpha1-->4) linkages. linkage analysis, periodate oxidation, and 1d/2d (1)h nmr spectroscopy of the int ... | 2008 | 18616317 |
| detection of bacterial contamination in cultures of eucaryotic cells by gas chromatography-mass spectrometry. | the use of gas chromatography-mass spectrometry for early detection of bacterial contaminations in cultures of baker's yeast, penicillium chrysogenum, and an animal cell line was evaluated; muramic acid and characteristic cellular fatty acids were used as analytes. by analyzing branched-chain and cyclopropane-substituted fatty acids as methyl esters, staphylococcus epidermidis, bacillus subtilis, lactobacillus reuteri, enterobacter cloacae, and pseudomonas fluorescens were detected in a 500-fold ... | 1993 | 18613045 |
| in vitro screening of probiotic characteristics of some italian products. | six commercial probiotic products produced and marketed italy (containing lactobacillus gg, lactobacillus casei dg, lactobacillus reuteri, bacillus clausii spores, bifidobacterium longum and saccharomyces boulardii) were assayed for their stability during storage, acid, base and bile tolerance and adherence to human intestinal cells. results indicate that storage, even at conditions established by manufacturers, affects the microbial content of products based on b. longum and partially of that c ... | 2008 | 18606590 |
| evaluation of reuterin production in urogenital probiotic lactobacillus reuteri rc-14. | classified as a distinct species in 1980, lactobacillus reuteri strains have been used in probiotic formulations for intestinal and urogenital applications. in the former, the primary mechanism of action of l. reuteri sd2112 (atcc 55730) has been purported to be its ability to produce the antibiotic 3-hydroxypropionaldehyde (3-hpa), also known as reuterin. in the vagina, it has been postulated that probiotic lactobacillus reuteri rc-14 does not require reuterin production but mediates a restorat ... | 2008 | 18539802 |
| probiotics for children with diarrhea: an update. | this review focuses on the efficacy of probiotics for diarrhea in children in different settings: day-care centers, diarrhea acquired in the hospital, antibiotic-associated diarrhea, and treatment of acute infectious diarrhea. for prevention of diarrhea acquired in day-care centers, 5 randomized and placebo-controlled trials have been published. probiotics tested were lactobacillus gg, bifidobacterium lactis (alone or in combination with streptococcus thermophilus), and lactobacillus reuteri. th ... | 2008 | 18520336 |
| high-level expression of recombinant beta-galactosidases in lactobacillus plantarum and lactobacillus sakei using a sakacin p-based expression system. | this work presents the cloning and expression of the genes encoding heterodimeric beta-galactosidases from lactobacillus reuteri l103, lactobacillus acidophilus r22, lactobacillus plantarum wcfs1, and lactobacillus sakei lb790. these enzymes consist of two subunits of approximately 73 and 35 kda, which are encoded by two overlapping genes, lacl and lacm, respectively. we have cloned these genes into the lactobacillal expression vectors psip403 and psip409, which are based on the sakacin p operon ... | 2008 | 18512940 |
| rapid identification of the three major species of dairy obligate heterofermenters lactobacillus brevis, lactobacillus fermentum and lactobacillus parabuchneri by species-specific duplex pcr. | in this study, the biodiversity of 154 strains of lactic acid bacteria, including 112 dairy product isolates presumptively identified as obligately heterofermentative lactobacilli (ohl) by classical microbiological tests, as well as 23 ohl-type strains, was investigated by pcr-based methods and gene sequencing. using these techniques, 51% of the cheese isolates were actually identified as ohl. the non-ohl isolates were identified to the leuconostoc, lactobacillus, weisella, pediococcus or strept ... | 2008 | 18510560 |
| the effects of probiotics on feeding tolerance, bowel habits, and gastrointestinal motility in preterm newborns. | to investigate the effect of dietary supplementation with a probiotic on feeding tolerance and gastrointestinal motility in healthy formula-fed preterm infants. | 2008 | 18492520 |
| comparative genome analysis of lactobacillus reuteri and lactobacillus fermentum reveal a genomic island for reuterin and cobalamin production. | lactobacillus reuteri is a heterofermentative lactic acid bacterium that naturally inhabits the gut of humans and other animals. the probiotic effects of l. reuteri have been proposed to be largely associated with the production of the broad-spectrum antimicrobial compound reuterin during anaerobic metabolism of glycerol. we determined the complete genome sequences of the reuterin-producing l. reuteri jcm 1112(t) and its closely related species lactobacillus fermentum ifo 3956. both are in the s ... | 2008 | 18487258 |
| lactobacillus reuteri dsm 20016 produces cobalamin-dependent diol dehydratase in metabolosomes and metabolizes 1,2-propanediol by disproportionation. | a lactobacillus reuteri strain isolated from sourdough is known to produce the vitamin cobalamin. the organism requires this for glycerol cofermentation by a cobalamin-dependent enzyme, usually termed glycerol dehydratase, in the synthesis of the antimicrobial substance reuterin. we show that the cobalamin-synthesizing capacity of another l. reuteri strain (20016, the type strain, isolated from the human gut and recently sequenced as f275) is genetically and phenotypically linked, as in the ente ... | 2008 | 18469107 |
| structural characterization of a human-type corrinoid adenosyltransferase confirms that coenzyme b12 is synthesized through a four-coordinate intermediate. | atp:cob(i)alamin adenosyltransferases (acas) catalyze the transfer of the 5'-deoxyadenosyl moiety from atp to the upper axial ligand position of cobalamin in the synthesis of coenzyme b 12. for the aca-catalyzed reaction to proceed, cob(ii)alamin must be reduced to cob(i)alamin in the enzyme active site. this reduction is facilitated through the generation of a four-coordinate cob(ii)alamin intermediate on the enzyme. we have determined the high-resolution crystal structure of a human-type aca f ... | 2008 | 18452306 |
| probiotic lactobacillus reuteri suppress proinflammatory cytokines via c-jun. | differential immunoregulatory capabilities of probiotic lactobacillus were explored in the context of pediatric crohn's disease. experimental strategies addressed molecular mechanisms of tumor necrosis factor (tnf) suppression in activated macrophages by transcriptional regulation. | 2008 | 18425802 |
| detection of t lymphocytes in intestine of broiler chicks treated with lactobacillus spp. and challenged with salmonella enterica serovar enteritidis. | the expression of immune response in the form of leukocytic infiltrate by cd3+, cd4+, and cd8+ cells in the epithelium and in the intestinal lamina propria of chicks was studied in the present work by means of immunohistochemical reaction. the chicks were treated with lactobacillus spp. or cecal microflora (cm) and experimentally challenged or not with salmonella enterica serovar enteritidis. the 320 birds utilized were divided into 4 groups containing 80 chicks each and submitted to treatments ... | 2008 | 18420983 |
| lactobacillus equigenerosi sp. nov., a coccoid species isolated from faeces of thoroughbred racehorses. | two strains of lactic acid bacteria were isolated from faeces of two actively racing thoroughbred horses. the isolates formed a subcluster in the lactobacillus reuteri phylogenetic group, closely related to lactobacillus fermentum, l. gastricus, l. ingluviei and l. mucosae, by phylogenetic analysis based on 16s rrna gene sequences. levels of dna-dna relatedness revealed that the isolates belonged to the same taxon and were genetically separated from their phylogenetic relatives. biochemical and ... | 2008 | 18398194 |
| human-derived probiotic lactobacillus reuteri demonstrate antimicrobial activities targeting diverse enteric bacterial pathogens. | lactobacillus reuteri is a commensal-derived anaerobic probiotic that resides in the human gastrointestinal tract. l. reuteri converts glycerol into a potent broad-spectrum antimicrobial compound, reuterin, which inhibits the growth of gram-positive and gram-negative bacteria. in this study, we compared four human-derived l. reuteri isolates (atcc 55730, atcc pta 6475, atcc pta 4659 and atcc pta 5289) in their ability to produce reuterin and to inhibit the growth of different enteric pathogens i ... | 2008 | 18396068 |
| identification of a surface protein from lactobacillus reuteri jcm1081 that adheres to porcine gastric mucin and human enterocyte-like ht-29 cells. | adhesion of lactobacilli to the host gastrointestinal (gi) tract is considered an important factor in health-promoting effects. however, studies addressing the molecular mechanisms of the adhesion of lactobacilli to the host gi tract have not yet been performed. the aim of this work was to identify lactobacillus reuteri surface molecules mediating adhesion to intestinal epithelial cells and mucins. nine strains of lactobacilli were tested for their ability to adhere to human enterocyte-like ht-2 ... | 2008 | 18379843 |
| repression of the locus of the enterocyte effacement-encoded regulator of gene transcription of escherichia coli o157:h7 by lactobacillus reuteri culture supernatants is luxs and strain dependent. | culture supernatants of lactobacillus reuteri atcc 55730 repressed ler expression in escherichia coli o157:h7 cells, but neither the strain's isogenic luxs mutant nor the l. reuteri 100-23c wild-type strain and its luxs mutant elicited a comparable effect. furthermore, the epinephrine-mediated induction of ler expression was repressed by secreted substance(s) of l. reuteri atcc 55730. | 2008 | 18378666 |
| production of lactose-free galacto-oligosaccharide mixtures: comparison of two cellobiose dehydrogenases for the selective oxidation of lactose to lactobionic acid. | galacto-oligosaccharides, complex mixtures of various sugars, are produced by transgalactosylation from lactose using beta-galactosidase and are of great interest for food and feed applications because of their prebiotic properties. most galacto-oligosaccharide preparations currently available in the market contain a significant amount of monosaccharides and lactose. the mixture of galacto-oligosaccharides (galos) in this study produced from lactose using recombinant beta-galactosidase from lact ... | 2008 | 18353295 |
| the effect of low ph on protein expression by the probiotic bacterium lactobacillus reuteri. | the ability of a lactic acid bacterium to survive passage through the gastrointestinal tract is a key point in its function as a probiotic. in this study, protein synthesis by the probiotic bacterium, lactobacillus reuteri, was analyzed under transiently decreased ph conditions. l. reuteri cells grown to the midexponential growth phase at 37 degrees c were exposed to transient (1 h) low-ph stresses from ph 6.8 to ph 5.0, 4.5, or 4.0. 2-de allowed us to identify 40 common proteins that were consi ... | 2008 | 18351691 |
| high-level folate production in fermented foods by the b12 producer lactobacillus reuteri jcm1112. | we observed that lactobacillus reuteri jcm1112 produces b(12) and folate. however, the folate/b(12) mass ratio found was far below that desired for human consumption ( approximately 170:1). we used metabolic engineering applying genetic and physiological approaches to improve this ratio and developed a generic and natural process that significantly increases folate production. | 2008 | 18344331 |
| probiotic lactobacillus reuteri promotes tnf-induced apoptosis in human myeloid leukemia-derived cells by modulation of nf-kappab and mapk signalling. | the molecular mechanisms of pro-apoptotic effects of human-derived lactobacillus reuteri atcc pta 6475 were investigated in this study. l. reuteri secretes factors that potentiate apoptosis in myeloid leukemia-derived cells induced by tumour necrosis factor (tnf), as indicated by intracellular esterase activity, terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end-labelling assays and poly (adp-ribose) polymerase cleavage. l. reuteri downregulated nuclear factor-kapp ... | 2008 | 18331465 |
| inhibition of helicobacter pylori infection in humans by lactobacillus reuteri atcc 55730 and effect on eradication therapy: a pilot study. | several studies report an inhibitory effect of probiotics on helicobacter pylori. aim: to test whether lactobacillus reuteri atcc 55730 reduces h. pylori intragastric load in vivo, decreases dyspeptic symptoms, and affects eradication rates after conventional treatment. | 2008 | 18321302 |
| structural analysis of the alpha-d-glucan (eps35-5) produced by the lactobacillus reuteri strain 35-5 glucansucrase gtfa enzyme. | the neutral exopolysaccharide eps35-5 (reuteran) produced from sucrose by the glucansucrase gtfa enzyme from lactobacillus reuteri 35-5 was found to be a (1-->4,1-->6)-alpha-d-glucan, with no repeating units present. based on linkage analysis and 1d/2d 1h and 13c nmr spectroscopy of intact eps35-5, as well as ms and nmr analysis of oligosaccharides obtained by partial acid hydrolysis and enzymatic hydrolysis, using pullulanase m1 (klebsiella planticola), of eps35-5, a composite model, that inclu ... | 2008 | 18313039 |
| structural analysis of the alpha-d-glucan (eps180) produced by the lactobacillus reuteri strain 180 glucansucrase gtf180 enzyme. | the neutral exopolysaccharide eps180 produced from sucrose by the glucansucrase gtf180 enzyme from lactobacillus reuteri 180 was found to be a (1-->3,1-->6)-alpha-d-glucan, with no repeating units present. based on linkage analysis, periodate oxidation, and 1d/2d 1h and 13c nmr spectroscopy of the intact eps180, as well as ms and nmr analysis of oligosaccharides obtained by partial acid hydrolysis of eps180, a composite model, that includes all identified structural features, was formulated as f ... | 2008 | 18313038 |
| genomic and genetic characterization of the bile stress response of probiotic lactobacillus reuteri atcc 55730. | probiotic bacteria encounter various stresses after ingestion by the host, including exposure to the low ph in the stomach and bile in the small intestine. the probiotic microorganism lactobacillus reuteri atcc 55730 has previously been shown to survive in the human small intestine. to address how l. reuteri can resist bile stress, we performed microarray experiments to determine gene expression changes that occur when the organism is exposed to physiological concentrations of bile. a wide varie ... | 2008 | 18245259 |
| low breast milk tgf-beta2 is induced by lactobacillus reuteri supplementation and associates with reduced risk of sensitization during infancy. | the immunological composition of breast milk differs between mothers. the reasons for these differences and the consequences for the breast-fed infants are poorly understood. the aim of this study was to evaluate the effect of probiotic lactobacillus reuteri supplementation on the immunological composition of breast milk in relation to sensitization and eczema in the babies. total iga, secretory iga (siga), tgf-beta1, tgf-beta2, il-10, tnf, soluble cd14 (scd14), and na/k ratios were analyzed in ... | 2008 | 18221472 |
| identification of lactic acid bacteria from poultry feces: evidence on anti-campylobacter and anti-listeria activities. | forty-five strains of lactic acid bacteria were isolated from poultry feces sampled from an industrial farm located in the nantes area (france), and most of them belong to the streptococcus or lactobacillus genus according to the results obtained by galerie api and molecular methods. the most representative lactic acid bacteria strains were screened for antibacterial compound production against 2 indicator organisms (listeria innocua f and campylobacter jejuni 11168) by means of the agar diffusi ... | 2008 | 18212377 |
| lactobacillus reuteri crl 1100 as starter culture for wheat dough fermentation. | the effect of sucrose on the fermentation balance of lactobacillus reuteri crl 1100 and the invertase activity of this strain in wheat dough and culture medium (mrss) was evaluated. the enzyme activity was dependent on the environmental ph releasing glucose and fructose from sucrose hydrolysis. glucose was used as carbon source, while fructose was mainly used as electron acceptor to produce mannitol up to 10h of fermentation. thereafter, fructose seemed to be metabolized by the heterofermentativ ... | 2008 | 18206767 |
| isolation and characterization of human intestinal bacteria capable of transforming the dietary carcinogen 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine. | 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (phip) is a carcinogenic heterocyclic aromatic amine formed in meat products during cooking. although the formation of hazardous phip metabolites by mammalian enzymes has been extensively reported, research on the putative involvement of the human intestinal microbiota in phip metabolism remains scarce. in this study, the in vitro conversion of phip into its microbial derivate, 7-hydroxy-5-methyl-3-phenyl-6,7,8,9-tetrahydropyrido[3',2':4,5]imidazo[ ... | 2008 | 18192423 |
| the complete coenzyme b12 biosynthesis gene cluster of lactobacillus reuteri crl1098. | the coenzyme b(12) production pathway in lactobacillus reuteri has been deduced using a combination of genetic, biochemical and bioinformatics approaches. the coenzyme b(12) gene cluster of lb. reuteri crl1098 has the unique feature of clustering together the cbi, cob and hem genes. it consists of 29 orfs encoding the complete enzymic machinery necessary for de novo biosynthesis. transcriptional analysis showed it to be expressed as two tandem transcripts of approximately 22 and 4 kb, carrying c ... | 2008 | 18174128 |
| glucosyltransferase a (gtfa) and inulosucrase (inu) of lactobacillus reuteri tmw1.106 contribute to cell aggregation, in vitro biofilm formation, and colonization of the mouse gastrointestinal tract. | members of the genus lactobacillus are common inhabitants of the proximal gastrointestinal tract of animals such as mice, rats, chickens and pigs, where they form epithelial biofilms. little is known about the traits that facilitate biofilm formation and gut colonization. this study investigated the ecological role of a glucosyltransferase (gtfa) and inulosucrase (inu) of lactobacillus reuteri tmw1.106 and a fructosyltransferase (ftfa) of l. reuteri lth5448. in vitro experiments using isogenic m ... | 2008 | 18174127 |
| a probiotic lozenge administered medical device and its effect on salivary mutans streptococci and lactobacilli. | previous studies have suggested that lactobacilli-derived probiotics in dairy products may affect oral ecology, but the effects of different delivery methods have received little attention. | 2008 | 18086024 |
| characterization of dominant cultivable lactobacilli and their antibiotic resistance profiles from faecal samples of weaning piglets. | to examine the lactic acid bacteria flora of weaning piglets, to define the distribution of different lactobacilli species in piglet faecal samples, and to determine the susceptibility phenotype to 11 antibiotic of different families. | 2007 | 18045434 |
| characterization of the structural gene encoding nisin f, a new lantibiotic produced by a lactococcus lactis subsp. lactis isolate from freshwater catfish (clarias gariepinus). | lactococcus lactis f10, isolated from freshwater catfish, produces a bacteriocin (bacf) active against staphylococcus aureus, staphylococcus carnosus, lactobacillus curvatus, lactobacillus plantarum, and lactobacillus reuteri. the operon encoding bacf is located on a plasmid. sequencing of the structural gene revealed no homology to other nisin genes. nisin f is described. | 2008 | 18039827 |
| genetic features of circular bacteriocins produced by gram-positive bacteria. | this review highlights the main genetic features of circular bacteriocins, which require the co-ordinated expression of several genetic determinants. in general terms, it has been demonstrated that the expression of such structural genes must be combined with the activity of proteins involved in maturation (cleavage/circularization) and secretion outside the cell via different transporter systems, as well as multifaceted immunity mechanisms essential to ensuring the bacteria's self-protection ag ... | 2008 | 18034824 |
| glycerol induces reuterin production and decreases escherichia coli population in an in vitro model of colonic fermentation with immobilized human feces. | lactobacillus reuteri atcc 55730 is a probiotic strain that produces, in the presence of glycerol, reuterin, a broad-spectrum antimicrobial substance. this strain has been shown to prevent intestinal infections in vivo; however, its mechanisms of action, and more specifically whether reuterin production occurs within the intestinal tract, are not known. in this study, the effects of l. reuteri atcc 55730 on intestinal microbiota and its capacity to secrete reuterin from glycerol in a novel in vi ... | 2008 | 18028400 |
| inhibitory activity spectrum of reuterin produced by lactobacillus reuteri against intestinal bacteria. | reuterin produced from glycerol by lactobacillus reuteri, a normal inhabitant of the human intestine, is a broad-spectrum antimicrobial agent. it has been postulated that reuterin could play a role in the probiotic effects of lb. reuteri. reuterin is active toward enteropathogens, yeasts, fungi, protozoa and viruses, but its effect on commensal intestinal bacteria is unknown. moreover reuterin's mode of action has not yet been elucidated. glutathione, a powerful antioxidant, which also plays a k ... | 2007 | 17997816 |
| isolation and characterization of lactobacillus species having potential for use as probiotic cultures for dogs. | the need to control pathogenic microorganisms in the intestinal tract of dogs is a growing concern. there is interest in using probiotics such as species of lactobacillus to help control canine intestinal infections. for successful use as a probiotic, the bacterial species should be of canine intestinal origin since these species exhibit host specificity. serial dilutions of freshly voided dog feces were plated on lactobacillus selection (lbs) agar to isolate the cultures. isolates were identifi ... | 2007 | 17995807 |
| influence of sodium glycocholate on production of conjugated linoleic acid by cells of lactobacillus reuteri atcc 55739. | the possible influence of a bile salt on production of conjugated linoleic acid (cla) by lactobacillus reuteri atcc 55739 was evaluated. cells of the lactobacilli grown in mrs broth with and without linoleic acid (la, 0.2%) were harvested and washed. the washed cells were added to buffer containing 0.2% la and incubated 18 h at 37 degrees c. the cells, which had been grown without la, transformed la into cla (mainly c9t11-c18:2) better than did those cells grown with it. when sodium glycocholate ... | 2007 | 17995782 |
| structural and functional analyses of the human-type corrinoid adenosyltransferase (pduo) from lactobacillus reuteri. | atp:co(i)rrinoid adenosyltransferase (aca) catalyzes the conversion of cobalamin to coenzyme b12, an essential cofactor in animal metabolism. several mutations of conserved residues in the active site of human aca have been identified in humans with methylmalonic aciduria. however, the catalytic role of these residues remains unclear. to better understand the function of these residues and to determine how the enzyme promotes catalysis, several variants of a human-type aca from the lactic acid b ... | 2007 | 17988155 |
| phosphoketolase pathway dominates in lactobacillus reuteri atcc 55730 containing dual pathways for glycolysis. | metabolic flux analysis indicated that the heterofermentative lactobacillus reuteri strain atcc 55730 uses both the embden-meyerhof pathway (emp) and phosphoketolase pathway (pkp) when glucose or sucrose is converted into the three-carbon intermediate stage of glycolysis. in all cases studied, the main flux is through the pkp, while the emp is used as a shunt. in the exponential growth phase, 70%, 73%, and 84% of the flux goes through the pkp in cells metabolizing (i) glucose plus fructose, (ii) ... | 2008 | 17965151 |
| pseudovitamin b(12) is the corrinoid produced by lactobacillus reuteri crl1098 under anaerobic conditions. | we have reported previously on the ability of lactobacillus reuteri to produce a compound with vitamin b(12) activity. here we report on the chemical characterisation of this corrinoid-like molecule. high performance liquid chromatography coupled to an ultraviolet diode array detector, mass spectrometry and nuclear magnetic resonance spectroscopy has enabled us to identify the compound as coalpha-[alpha-(7-adenyl)]-cobeta-cyanocobamide or pseudovitamin b(12). this molecule differs from cobalamin ... | 2007 | 17888910 |
| modifying effects of fermented brown rice on fecal microbiota in rats. | brown rice fermented by aspergillus oryzae (fbra) is a fiber-rich food. effects of dietary administration of fbra on rat fecal microbiota composition were examined. male wistar rats were fed a basal diet or a 5% fbra- or 10% fbra-containing diet, and fecal microbiota was analyzed by culture and terminal-restriction fragment length polymorphism (t-rflp) analysis. the viable cell number of lactobacilli significantly increased after feeding 10% fbra diet for 3 weeks compared with that in the basal ... | 2007 | 17826198 |
| dietary supplementation with sorbitol results in selective enrichment of lactobacilli in rat intestine. | a potential prebiotic action has been ascribed to sorbitol, but in vivo evidence of this remains scarce. in the present work, the effect of sorbitol was compared to that of fructo-oligosaccharides (fos) in a rat model. microbiota changes, particularly in lactobacilli, were analyzed on fecal, colonic and cecal samples. denaturing gradient gel electrophoresis (dgge) analysis of 16s rrna gene amplicons using universal primers showed that fos and sorbitol diets exerted a strong influence upon gut mi ... | 2007 | 17825531 |
| evaluation of an intestinal lactobacillus reuteri strain expressing rumen fungal xylanase as a probiotic for broiler chickens fed on a wheat-based diet. | 1. this study was conducted to evaluate the feasibility of using a transformed lactobacillus reuteri pg4 strain harbouring a rumen fungal xylanase gene as a probiotic supplement in a wheat-based poultry diet. 2. a total of 400 broiler chicks was allocated to two treatment groups with or without supplementation with 10(6) colony forming units (cfu)/g of transformed l. reuteri pg4 in a wheat-based regimen to investigate the performance, intestinal microflora populations, digesta viscosity and excr ... | 2007 | 17701504 |
| lactobacillus reuteri tablets suppress helicobacter pylori infection--a double-blind randomised placebo-controlled cross-over clinical study. | we studied the effect of lactobacillus reuteri strain sd2112 tablets reuterina (erina co., inc.), in suppressing h. pylori urease activity and to use the urea breath test (ubt) as a marker for the burden of infection. method 1: assessment of ubt and h. pylori density. subjects were 33 h. pylori-positive patients from whom were obtained gastric biopsy specimens by upper gastrointestinal endoscopy. the correlation between ubt and h. pylori density was investigated. individual ubt was established f ... | 2007 | 17695792 |
| coexpression of rumen microbial beta-glucanase and xylanase genes in lactobacillus reuteri. | the aim of this study was to clone and coexpress two rumen fibrolytic enzyme genes in lactobacillus reuteri. the ability of the genetically modified strain to degrade beta-glucan and xylan was evaluated. the fibrobacter succinogenes beta-glucanase (1,3-1,4-beta-d: -glucan 4-glucanohydrolase [ec 3.2.1.73]) gene and the neocallimastix patriciarum xylanase gene, xyncdbfv, were constructed to coexpress and secrete under control of the lactococcus lactis laca promoter and its secretion signal and the ... | 2007 | 17694302 |
| influence of oligosaccharides on the viability and membrane properties of lactobacillus reuteri tmw1.106 during freeze-drying. | freeze-drying is a process commonly used in starter culture preparation. to improve the survival rate of bacteria during the process, cryoprotectives are usually added before freezing. this study investigated the influence of the addition of sucrose, fructo-oligosaccharides (fos), inulin and skim milk on the viability and membrane integrity of lactobacillus reuteri tmw1.106 during freezing, freeze-drying and storage. the effect of drying adjuncts on survival was correlated to their interaction w ... | 2007 | 17651717 |
| mice protected by oral immunization with lactobacillus reuteri secreting fusion protein of escherichia coli enterotoxin subunit protein. | a green fluorescent protein (gfp) gene was ligated to the lactobacillus reuteri-specific nisin-inducible expression-secretion vector pnies, generating a pnies-gfp vector capable of secreting the cloned gene as a gfp-fusion protein with fluorescent activity. to develop this system as a live vehicle carrying the heat-stable enterotoxin (st) and heat-labile enterotoxin b (lt(b)) of the enterotoxigenic escherichia coli (etec), a recombinant 5'-st-lt(b)-3' dna fragment was cloned into pnies-gfp. the ... | 2007 | 17651125 |
| comparison between discontinuous and continuous lactose conversion processes for the production of prebiotic galacto-oligosaccharides using beta-galactosidase from lactobacillus reuteri. | galacto-oligosaccharide (gos) formation from lactose in discontinuous and continuous modes of conversion was investigated using beta-galactosidase (beta-gal) from lactobacillus reuteri. a continuous stirred tank reactor (cstr) with an external crossflow membrane was set up, and continuous gos production was analyzed and compared to the batchwise formed gos product. marked differences were detected for the two reactor setups. above 65% lactose conversion, the gos yield was lower for the cstr due ... | 2007 | 17630761 |
| freezing resistance improvement of lactobacillus reuteri by using cell immobilization. | lactobacillus reuteri shows certain beneficial effects to human health and is recognized as a probiotic. however, its application in frozen foods is still not popular because of its low survival during freezing and frozen storage. cell immobilization technique could effectively exert protection effects to microbial cells in order to enhance their endurance to unfavorable environmental conditions as well as to improve their viability and cell concentration. ca-alginate and kappa-carrageenan were ... | 2007 | 17617480 |
| probiotics in infectious diarrhoea in children: are they indicated? | infectious gastroenteritis continues to be a leading cause of mortality and morbidity worldwide. the cornerstone of treatment remains replacement of water and electrolyte losses with oral rehydration solution. until a few years ago, probiotics were discussed primarily in the context of alternative medicine, but they are now entering mainstream medical practice since a decrease of the severity and duration of infectious gastroenteritis in approximately 24 hours has been shown for some strains. th ... | 2007 | 17611775 |
| d-alanyl ester depletion of teichoic acids in lactobacillus reuteri 100-23 results in impaired colonization of the mouse gastrointestinal tract. | the dlt operon of gram-positive bacteria encodes proteins required for the incorporation of d-alanine esters into cell wall-associated teichoic acids (ta). d-alanylation of ta has been shown to be important for acid tolerance, resistance to antimicrobial peptides, adhesion, biofilm formation, and virulence of a variety of pathogenic organisms. the aim of this study was to determine the importance of d-alanylation for colonization of the gastrointestinal tract by lactobacillus reuteri 100-23. ins ... | 2007 | 17564608 |
| differential inflammatory mediator response in vitro from murine macrophages to lactobacilli and pathogenic intestinal bacteria. | chronic active colitis (including inflammatory bowel disease - ibd) is maintained by a variety of pro-inflammatory mediators. certain intestinal bacterial strains may induce colitis, whereas some strains (e.g. lactobacillus spp.) show a protective effect in colitis owing to their anti-inflammatory activity. in this study, we have examined the production of selected inflammatory cytokines, reactive oxygen species (ros), nitric oxide (no) and the expression of haeme oxygenase-1 (ho-1) by murine pe ... | 2007 | 17504445 |
| glutathione reductase from lactobacillus sanfranciscensis dsm20451t: contribution to oxygen tolerance and thiol exchange reactions in wheat sourdoughs. | the effect of the glutathione reductase (gshr) activity of lactobacillus sanfranciscensis dsm20451(t) on the thiol levels in fermented sourdoughs was determined, and the oxygen tolerance of the strain was also determined. the gshr gene coding for a putative gshr was sequenced and inactivated by single-crossover integration to yield strain l. sanfranciscensis dsm20451(t)deltagshr. the gene disruption was verified by sequencing the truncated gshr and surrounding regions on the chromosome. the gshr ... | 2007 | 17496130 |
| sucrose utilization and impact of sucrose on glycosyltransferase expression in lactobacillus reuteri. | glycosyltransferases of lactic acid bacteria are associated with biofilm formation, bacterial stress response and sucrose metabolism. the aim of this study was to determine the contribution of glycosyltransferases to sucrose metabolism in lactobacillus reuteri tmw1.106 expressing the glucosyltransferase gtfa and the inulosucrase inu, and l. reuteri lth 5448 expressing the fructosyltransferase ftfa. transcriptional analysis using quantitative real time pcr revealed that expression of ftfa of l. r ... | 2007 | 17490840 |
| specific bifidobacterium strains isolated from elderly subjects inhibit growth of staphylococcus aureus. | cell-free, ph-controlled supernatants of thirty-eight bifidobacterium strains isolated from healthy elderly subjects were subjected to antimicrobial activity assay. bioluminescent indicator strains staphylococcus aureus rn4220, escherichia coli k-12, and salmonella enterica serovar typhimurium atcc 14028 were used as targets of antimicrobial activity. the effect of nutrient depletion on the inhibition was eliminated with spent-culture controls. three out of thirty-eight bifidobacterium strains w ... | 2007 | 17462772 |
| the early response to acid shock in lactobacillus reuteri involves the clpl chaperone and a putative cell wall-altering esterase. | to be able to function as a probiotic, bacteria have to survive the passage through the gastrointestinal tract. we have examined survival and gene expression of lactobacillus reuteri atcc 55730 after a sudden shift in environmental acidity to a ph close to the conditions in the human stomach. more than 80% of the l. reuteri cells survived at ph 2.7 for 1 h. a genomewide expression analysis experiment using microarrays displayed 72 differentially expressed genes at this ph. the early response to ... | 2007 | 17449683 |
| dgge and 16s rdna sequencing analysis of bacterial communities in colon content and feces of pigs fed whole crop rice. | the effect of feeding whole crop rice (wcr) to growing-finishing pigs at three levels 0 (control), 10% and 20% on bacterial communities in colon content and feces was analyzed using 16s rdna-based techniques. amplicons of the v6-v8 variable regions of bacterial 16s rdna were analyzed by denaturing gradient gel electrophoresis (dgge), cloning and sequencing. the total number of dgge bands and shannon index of diversity for feces samples were higher in the pigs fed wcr-containing diets compared wi ... | 2007 | 17446093 |
| lactobacillus secaliphilus sp. nov., isolated from type ii sourdough fermentation. | two strains of gram-positive, catalase-negative, lactic acid bacteria, strains tmw 1.1309(t) and tmw 1.1313, were isolated at an interval of several years from an industrial type ii sourdough. they occurred at cell numbers of 8x10(8) c.f.u. g(-1) and therefore were considered to be one of the dominant members of the microbiota in this type of fermentation. cells of both strains grow exclusively on modified mrs containing trypsin-digested rye-bran extract. both strains possessed identical 16s rrn ... | 2007 | 17392199 |
| survival and persistence of lactobacillus plantarum 4.1 and lactobacillus reuteri 3s7 in the gastrointestinal tract of pigs. | lactobacillus sp. are important inhabitants of the intestines of animals. they are also largely used as probiotics for both humans and animals. to exert beneficial effects, lactobacilli have to survive through the gastrointestinal transit. based on bile-salt resistance, ph tolerance, antimicrobial activity and heat resistance, lactobacillus plantarum 4.1 and lactobacillus reuteri 3s7 were previously selected and used as probiotic additives in pelleted feeding trials. both strains were fed to pig ... | 2007 | 17391871 |
| species diversity and relative abundance of vaginal lactic acid bacteria from women in uganda and korea. | lactobacilli play an important role in maintaining vaginal health of women. the aim of this study was to compare the species richness and relative abundance of lactobacillus and other lactic acid bacteria in women of two geographically distant countries, uganda and korea. | 2007 | 17381754 |
| identification of lactobacilli isolated from the cloaca and vagina of laying hens and characterization for potential use as probiotics to control salmonella enteritidis. | to select lactobacillus strains from laying hens for potential use as probiotic to control salmonella enteritidis infection. | 2007 | 17381753 |