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[pyruvate oxidase gene from streptococcus sanguis: molecular cloning and sequence analysis of the gene].to clone and sequence the gene of pyruvate oxidase (sopox) from streptococcus sanguis.200112536558
correlations between bacterial levels in autologous subgingival plaque and saliva of adult sudanese.the aim of this study was to assess levels of oral bacteria and their correlations in paired samples of saliva and subgingival plaque in a population of adult sudanese. whole saliva and pooled subgingival plaque samples from six probing sites of one tooth in each jaw were obtained from 56 sudanese adults (mean age 35.2+/-8.9 years). levels of 24 oral bacteria in the autologous saliva and pooled plaque sample of each subject were assessed by dna probes and checkerboard dna-dna hybridization. ther ...200212483235
bactericidal effect of the er:yag laser on dental implant surfaces: an in vitro study.the aim of the in vitro study was to examine the bactericidal effect of an er:yag laser on common dental implant surfaces.200212479633
selective lethal photosensitization of methicillin-resistant staphylococcus aureus using an igg-tin (iv) chlorin e6 conjugate.the growing resistance of methicillin-resistant staphylococcus aureus (mrsa) to conventional antimicrobial agents necessitates the development of alternative approaches to preventing and treating infections. one such approach is photodynamic therapy, whereby target cells are treated with light-activated drugs (photosensitizers). this investigation aimed to determine whether the ability of mrsa to express the igg-binding protein, protein a, could be exploited to enable selective lethal photosensi ...200212461004
increased proliferative response of peripheral blood mononuclear cells and t cells to streptococcus mutans and glucosyltransferase d antigens in the exacerbation stage of recurrent aphthous ulcerations.cytotoxic t lymphocyte-induced lysis of virus-infected oral epithelial cells has been shown to be a cause of early ulcer formation in recurrent aphthous ulcerations (rau). to test whether bacteria and their associated antigens are involved in the disease process of rau, the proliferative response (pr) to different streptococcal species in peripheral blood mononuclear cells (pbmc) and t cells isolated from rau patients at the exacerbation stage was determined.200212440086
a retrospective investigation of advanced periodontal disease as a risk factor for septicemia in hematopoietic stem cell and bone marrow transplant recipients.septicemia is a cause of death in hematopoietic stem cell transplant (hsct) recipients. extraction of teeth with advanced periodontitis has been advocated before hsct to prevent septicemia in myeloablated hosts. the primary aim of the present study was to determine impact of chronic periodontitis, as measured by radiographic alveolar bone loss, on septicemia and transplant mortality.200212424452
the relativity between streptococcus sanguis group and coronary heart disease.to study the relativity between streptococcus sanguis group (ssg) and coronary heart disease (chd).200212419151
the effect of para-aminobenzoic acid on growth and metabolism of porphyromonas gingivalis.to study the effect of para-aminobenzoic acid (paba) on the growth and metabolism of porphyromonas gingivalis (p.g).200212411175
monocytes maintain tissue factor activity after cytolysis of bacteria-infected endothelial cells in an in vitro model of bacterial endocarditis.intravascular infection with staphylococcus aureus, staphylococcus epidermidis, or streptococcus sanguis can initiate fibrin formation on endocardial tissue, causing bacterial endocarditis. the ability of these bacteria to injure intact endothelial cells (ecs) and to aggravate tissue factor (tf)-dependent coagulation in the presence of blood leukocytes was investigated. cytolysis of ecs occurred after infection with s. aureus and, with membrane-bound monocytes or granulocytes present, also after ...200212355366
streptococcus sanguis meningitis: report of a case.a 35-year-old woman who presented with acute purulent meningitis and hearing loss was reported. no bacteria was seen with gram' s stain of cerebrospinal fluid (csf). csf grew streptococcus viridans, which was subsequently identified to be s. sanguis. the patient improved after treatment, including hearing.200212296418
in vitro study of transmission of bacteria from contaminated metal models to stone models via impressions.the purpose of this study was to determine the risk of microbiological transmission into the dental laboratory via impressions and dental stone models. metal master models were contaminated with bacillus subtilis and streptococcus sanguis. impressions in alginate, polyvinylsiloxane, and polyether were used, and models were cast in two brands of dental stone. samples were taken from the impression surfaces before and after casting, and sections from the stone models were taken by a technique deve ...200212271350
[microbiological validation of the choice of basic plastic for removable dentures].adhesion of obligate and facultative anaerobic bacteria, favoring the development of oral inflammatory diseases, including the cariesogenic and periodontogenic bacteria and candida albicans fungi, isolated from patients with periodontitis, to 13 basic materials used in removable denture making, was studied. the adhesion of all bacteria (streptococcus sanguis, prevotella melangogenica, fusobacterium nucleatum, corynebacterium xerosis) and fungi to hot polymerization basic materials was the maximu ...200212224327
enhanced neutrophil emigration and porphyromonas gingivalis reduction following pgg-glucan treatment of mice.periodontal disease is the consequence of a mixed gram-negative infection in the gingival sulcus and has been associated with deficits in the neutrophil response. a novel, and heretofore untested, alternative approach to therapy is the use of biological-response modulators that enhance the neutrophil response. poly-beta1-6-glucotriosyl-beta1-3-glucopyranose glucan (pgg-glucan) is an immunomodulator, derived from yeast, which specifically enhances neutrophil priming, phagocytosis and bacterial ki ...200212221019
[study on the biosynthesis of para-aminobenzoic acid by s. sanguis. ii. qualitative and quantitative analysis].in this study, the method established in paper 1 was used to analyze paba synthesized by s. sanguis s34. the results showed: 1. s. sanguis s34 did synthesize paba and the mean concentration of paba was 1.236 micrograms/ml. 2. the components had good linear relation and average recovery was 80%. the results of this paper will be helpful to studying influencing factors on paba synthesis of s. sanguis and the roles of paba in microbial homeostasis of dental plaque.199812214421
[study on the biosynthesis of para-aminobenzoic acid by s. sanguis. i. establishment of a reversed-phase high-performance liquid chromatography].in order to study the synthesis of para-aminobenzoic acid (paba) by s. mutans and the role of paba in the interaction between s. mutans and s. sanguis, a reversed-phase high-performance liquid chromatography (rp-hplc) for analysis of paba was developed. the results showed that the optimal chromatographic parameters were flow rate of 1.5 ml/min, t = 55 +/- 2 degrees c, methanol/phosphate buffer of 10/90(by vol) (ph 2.2 0.1 mol/l), m-hydroxybenzoic acid as an internal standard. the method is simpl ...199812214420
[research on inhibition of sodium fluoride on five subgingival bacteria in vitro].this in vitro study was aimed at observing inhibition action of fluoride on 5 common periodontal bacteria in subgingival microflora. the liquid media two-fold dilution method was used to determine the minimal inhibition concentration (mic) of sodium fluoride (naf) to 9 strains of bacteria representing the main periodontopathic species(p. gingivalis, a. actinomycetemcomitans, p. intermedius) and the periodontal beneficial species (s. sanguis) and 1 strain of s. sorbrinus. the results showed that ...199812214405
groesl sequence determination, phylogenetic analysis, and species differentiation for viridans group streptococci.the full-length sequences of the groesl genes (also known as cpn10/60) of streptococcus anginosus, streptococcus constellatus, streptococcus gordonii, and streptococcus sanguis and the near full-length sequence of the groesl genes of streptococcus intermedius, streptococcus bovis, streptococcus mitis, streptococcus mutans, streptococcus oralis, and streptococcus salivarius were determined. the lengths of the groes genes from the 10 species listed above ranged from 282 to 288 bp, and the full-len ...200212202549
periodontitis as a risk factor for cardiovascular disease. 200012167894
streptococcus sanguis modulates type ii collagen-induced arthritis in dba/1j mice.native type ii collagen is tolerogenic when given orally or i.p. to dba/1j mice and induces autoimmune arthritis when given s.c. in cfa. the tolerogenic epitope is contained in cyanogen bromide fragment 11 (cb11) and is structurally mimicked by pgeqgpk within the platelet aggregation-associated protein (paap) on streptococcus sanguis. to learn whether s. sanguis modulates transmucosally the ag-specific development of autoimmune arthritis, dba/1j pups were given live s. sanguis, cb11, or type ii ...200212165549
the relationship between odontogenic bacteraemia and orthodontic treatment procedures.the purpose of this research was to estimate the prevalence and intensity of bacteraemia associated with orthodontic treatment procedures. the four procedures investigated were: an upper alginate impression, separator placement, band placement, and adjustment of an archwire on a fixed appliance. eighty-one children undergoing general anaesthesia (ga) for dento-alveolar surgery related to their orthodontic treatment were randomly allocated to the impression or separator group. a further 61 childr ...200212143093
intracellular and extracellular phs of streptococcus mutans after addition of acids: loading and efflux of a fluorescent ph indicator in streptococcal cells.a ph-sensitive fluorescent dye, 2', 7'-bis-(2-carboxyethyl)-5 and 6-carboxyfluorescein (bcecf), was used to determine intracellular ph (ph(in)). the efflux of bcecf loaded into oral streptococcal cells was determined after incubation of the cells at 35 degrees c for 20 min in the presence and absence of glucose. in the absence of glucose, the fluorescence of intracellular bcecf in streptococcus mutans, streptococcus sanguis, streptococcus salivarius and streptococcus sobrinus decreased only very ...200212121474
influence of secondary colonizers and human plasma on the adherence of porphyromonas gingivalis in vitro.the influence of secondary colonizers (fusobacterium nucleatum and actinomyces naeslundii) and the effect of human plasma on the adherence of porphyromonas gingivalis were investigated. hydroxyapatite (hap) discs coated with streptococcus sanguis were immersed in a 3h-labeled bacterial cell suspension of f. nucleatum or a. naeslundii and then in a 14c-labeled p. gingivalis cell suspension. bacterial cells on the discs were pyrolysed to quantify the radioisotopes released. the cell numbers of sec ...200212120708
septic arthritis due to streptococcus sanguis.we report an unusual case of septic arthritis due to streptococcus sanguis, a member of the viridans group of streptococci that are part of the normal flora of the mouth and upper respiratory tract. our patient had severe underlying periodontal disease, which likely contributed to his joint sepsis through hematogenous spread. although viridans streptococci are rare causes of septic arthritis in native joints, they should be considered in the setting of severe periodontal disease.200212108609
roles of salivary proteins in the adherence of oral streptococci to various orthodontic brackets.knowledge of salivary pellicles on orthodontic brackets provides a better understanding of microbial adherence. the aim of this study was to analyze the effects of bracket pellicles on the adherence of streptococcus gordonii and streptococcus mutans. bracket pellicles were formed by the incubation of 4 kinds of orthodontic brackets with unstimulated whole saliva for 2 hrs, and analyzed by electrophoresis, immunodetection, and amino acid analysis. binding assays were then performed by the incubat ...200212097434
a role for glycoprotein ib in streptococcus sanguis-induced platelet aggregation.numerous studies have implicated bacteria in cardiovascular disease, but there is a paucity of information on the mechanism involved. in this study we show how the common oral bacterium streptococcus sanguis can directly interact with platelets, resulting in activation and aggregate formation. platelet aggregation was dependent on glycoprotein iib/iiia (gpiib/iiia) and thromboxane. platelets could also directly bind to s sanguis, but this interaction was not inhibited by gpiib/iiia antagonists. ...200212091342
henoch-schonlein purpura secondary to subacute bacterial endocarditis.henoch-schönlein purpura (hsp), a systemic, small-vessel vasculitic syndrome, is characterized by a nonthrombocytopenic purpuric rash, arthralgia, abdominal pain, and nephritis. these signs and symptoms may occur in any order, and not all are necessary for the diagnosis. although most common in 4- to 7-year-olds, hsp is well documented in adults and is often preceded by a history of mucosal-based infections, especially of the upper respiratory tract. we report a case of hsp that occurred coincid ...200212080945
[the study of precedent subgingival flora colonization on four kinds of crown and bridge restorative materials].the purpose of this in vitro study was to investigate the effects of microbial colonization on different crown or fixed bridge-used restorative materials and discuss the standards for evaluating those materials microscopically. four kinds of restorative materials such as sda-ii type medium alloy, ceramic-fused-to-metal material, heat cure composite resin for crown restoration, plat castable ceramics and four species of precedent subgingival flora (a. viscosus, f. nucleterm, c. ochracea, s. sangu ...199812078179
[clinical and laboratory studies of bacterial adhesion to validate the choice of material for making provisional dentures for patients with periodontal diseases].adhesion of bacteria favoring the development of oral inflammations, including cariesogenic and periodontopathogenic (actinobacillus actinomycetemcommitans, streptococcus sanguis, fusobacterium nucleatum, staphylococcus warneri) and yeast fungi (candida albicans), to 13 materials used for making provisional dentures was studied. adhesion of all the studied bacteria and fungi to russian material esterfil foto was the minimum. clinical use of this material in patients with chronic generalized peri ...200212056141
effects of glucose and fluoride on competition and metabolism within in vitro dental bacterial communities and biofilms.antimicrobial effects of fluoride in vivo remain contentious. previous studies suggested that 1 mm naf reduced acid production from glucose, and prevented the enrichment of bacteria associated with caries in a chemostat model. the present study examines the effects of a lower fluoride concentration (0.53 mm, 10 ppm naf) in both biofilm and planktonic microbial communities. nine oral species were grown at ph 7.0 and pulsed on 10 successive days with glucose; bacterial metabolism was allowed to re ...200212037363
the antimicrobial properties of a urea-based handwash lotion with triclosan.the purpose of this study was to determine and compare the antimicrobial action of a urea-based handwash lotion with triclosan with a lipid-depleting detergent-based handwash product with 4% chlorhexidine gluconate. the zones of inhibition produced by the two handwash lotions and the one produced by the positive control were similar against all strains of bacteria tested, indicating that a urea-based handwash lotion with triclosan is as effective as a lipid-depleting handwash lotion in inhibitin ...200112024756
simultaneous measurement of the viability, aggregation, and live and dead adherence of streptococcus crista, streptococcus mutans and actinobacillus actinomycetemcomitans in human saliva in relation to indices of caries, dental plaque and periodontal disease.salivary proteins have multiple functions and many share similar functions, which may be why it has been difficult to relate variations in their concentrations to oral health and ecology. an alternative is to focus on variations in the major functions of saliva. an hydroxyapatite-coated microplate model has been developed that simultaneously measures saliva-promoted bacterial viability, bacterial aggregation, and live and dead bacterial adherence, while simulating oral temperature and shearing f ...200212015215
particulate bioglass reduces the viability of bacterial biofilms formed on its surface in an in vitro model.45s5 bioglass is a bioactive implant material which, in its particulate form, is used in the repair of periodontal defects. the surface reactions undergone by this material in an aqueous environment may exert an antibacterial effect that would be beneficial to periodontal surgical treatment. biofilms of streptococcus sanguis, an early plaque former, and mixed species biofilms from a salivary inoculum grown under conditions similar to those associated with periodontal implants, were grown on part ...200212005145
asymmetric primary ringless repair of mitral valve endocarditis: a case report.the case is presented of a patient with a large mitral valve vegetation. a technique of mitral valve repair involving ringless annuloplasty repair following bileaflet resection of the infected regions of the valve is described. no pledgets or prosthetic material were used in the repair. the patient recovered uneventfully, with trivial mitral regurgitation documented on post-repair echocardiography. this technique may suggest a method for early intervention in mitral endocarditis.200212000175
[therapeutic impact of streptococcal and enterococcal bacteremia in hematology patients].from january 1999 to may 2000 (17 months), 21 strains of streptococci and four strains of enterococci have been isolated from 74 blood cultures in 25 infectious episodes in hematologic patients. they concerned 21 patients, of 21 to 77 years old. these patients suffered from acute leukaemia (14 cases), chronic lymphoid leukaemia (two cases), non-hodgkin's lymphoma (two cases) or myeloma (three cases). seventeen patients displayed a single streptococcal or enterococcal episode, two had two episode ...200211980330
fluoride and organic weak acids as respiration inhibitors for oral streptococci in acidified environments.oxygen metabolism (respiration) of streptococcus mutans gs-5 involving nadh oxidases, mainly of the h(2)o-producing type, was found to be acid sensitive, as was nadh oxidase activity of cell extracts. respiration of intact cells in acidified media was also highly sensitive to fluoride, with a 50% inhibitory concentration of about 0.02 mm at ph 4. in contrast, nadh oxidases in cell extracts were fluoride insensitive. fluoride inhibition of respiration of intact cells was related to weak-acid effe ...200211929560
subgingival microbiota of indigenous indians of central america.to define the subgingival microbial profiles of adult subjects from a previously identified rural community of indigenous indians in guatemala, central america.200211895544
streptococcus sinensis sp. nov., a novel species isolated from a patient with infective endocarditis.a bacterium was isolated from the blood culture of a patient with infective endocarditis. the cells were facultative anaerobic, nonsporulating, gram-positive cocci arranged in chains. the bacterium grows on sheep blood agar as alpha-hemolytic, gray colonies of 0.5 to 1 mm in diameter after 24 h of incubation at 37 degrees c in ambient air. growth also occurs in 10 or 40% bile and on bile esculin agar but not in 6% nacl. no enhancement of growth is observed in 5% co(2). it is nongroupable with la ...200211880397
the effect of tissue molecules on bacterial invasion of dentine.bacterial invasion of dentinal tubules is a critical step in the pathogenesis of dental caries and pulp and periapical disease. the purpose of this study was to determine the effect some molecules commonly found in saliva and dentinal tubule fluid may have on the bacterial invasion of dentine. the results showed that invasion of streptococcus gordonii or enterococcus faecalis cells was inhibited when the bacterial cells were in solution with mucin, immunoglobulin g (igg) and serum, and this was ...200211860553
initial characterization of the streptococcus gordonii htpx gene.examination of the streptococcus gordonii chromosomal region, which lies immediately upstream of the glucosyltransferase positive regulatory determinant rgg, revealed two open reading frames. based on nucleotide sequences, these genes were similar to the listeria monocytogenes lema gene, which is involved in antigen presentation, and the escherichia coli htpx heat shock gene, which has an unknown function. northern hybridization analysis indicated that s. gordonii lema and htpx genes were associ ...200211860552
in vitro activity of quinupristin/dalfopristin against selected bacterial pathogens isolated in italy.objective: to evaluate the activity of quinupristin/dalfopristin, a new injectable streptogramin, against 732 clinical strains recently isolated in italy. methods: susceptibility tests were performed according to nccls-guided mic methodology. pathogens included in the evaluation included 108 staphylococcus aureus isolates, 124 coagulase-negative staphylococcal isolates, 158 streptococcus pyogenes isolates, 30 streptococcus agalactiae isolates, 30 b-hemolytic streptococcal isolates, 18 streptococ ...199911856292
current status of taxonomic groups of oral streptococci in endocarditis. can virulence factors discriminate between endocarditis and non-endocarditis strains?objective: infective endocarditis is frequently caused by oral streptococci, especially streptococcus sanguis. in this group, many strains have recently been reclassified on the basis of new taxonomic schemes. the purpose of this study was to classify oral streptococci from patients with infective endocarditis and, further, to assess the importance of specific virulence factors for the development of streptococcal endocarditis. methods: twenty-eight previously identified and 10 new streptococcal ...199911856221
streptococcus sanguis secretes cd14-binding proteins that stimulate cytokine synthesis: a clue to the pathogenesis of infective (bacterial) endocarditis?streptococcus sanguis is the major causative organism of infective (bacterial) endocarditis but, surprisingly, almost nothing is known about how it induces endocardial inflammation. in earlier studies we have shown that many bacteria secrete potent cytokine-inducing or -inhibiting proteins. we have therefore isolated the material secreted by s. sanguis grown on blood agar or in broth culture and have tested its ability to induce human peripheral blood monocytes to synthesize pro-inflammatory cyt ...200211855941
a protocol for polymerase chain reaction detection of enterococcus faecalis and enterococcus faecium from the root canal.the present study was set up to develop a protocol for detection of enterococcus faecalis and enterococcus faecium from the root canal.200211853234
vitality status of microorganisms in infected human root dentine.this experimental study was initiated to establish a method for characterizing the vitality status of bacteria in infected human root dentine by differentiating between viable and dead microorganisms.200211843972
adhesion in vitro of oral streptococci to porcelain, composite resin cement and human enamel.we investigated the initial adhesion in vitro of oral streptococci to porcelain inlays. four strains of streptococci, streptococcus mitis nctc12261, streptococcus oralis atcc9811, streptococcus sanguis atcc10556 and streptococcus sobrinus omz176, were used in this study. the disc specimens were made of porcelain, composite resin cement and human enamel. these specimens, with or without a saliva-coat, were immersed in a suspension of each streptococci strain at 37 degrees c for 1 hr, and the numb ...200011816366
antimicrobial activity of four root canal sealers against endodontic pathogens.the antibacterial effects of various types of widely used endodontic sealers have not been compared systematically on facultative or obligate anaerobic endodontic pathogens. the aim of this study was to evaluate the antimicrobial properties of four commonly used endodontic sealers: two epoxy-resin-based sealers (ah26, ah plus), one zinc-oxide eugenol-based sealer (n2), and one calcium hydroxide-based sealer (sealapex). the testing microbes were four facultative anaerobic species (streptococcus m ...200111800436
er:yag and alexandrite laser radiation propagation in root canal and its effect on bacteria.the objective of this study was to compare the bactericidal effect of the er:yag (wavelength 2.94 microm) and the alexandrite (wavelength 0.75 microm) laser radiation. the spreading laser energy in the surrounding hard dental tissues round the root canal was evaluated and the bactericidal effect of both these different laser wavelengths was analyzed.199911800099
the predominant microflora of nursing caries lesions.the predominant microflora recovered from infected dentine of 52 carious teeth from 14 children with nursing caries was determined using both selective and non-selective media for the isolation of specific genera and acidified media (ph 5.2) to isolate the predominant aciduric microorganisms, and compared with the microflora of sound enamel surfaces in caries-free children. streptococcus mutans formed a significantly greater proportion of the lesion flora while streptococcus oralis, streptococcu ...200111799279
[clubbed fingers]. 200111794900
[regulation of adherence to serum-coated hydroxyapatite by streptococcus sanguis].to investigate the effects of ph and calcium iron on adhesion of streptococcus sanguis to serum-coated hydroxyapatite.199911776933
[implication and mode of action of infectious agents in the formation of atheromatous plaques. infection and atherosclerosis].different kinds of infectious agents seem to be implied in the atherosclerotic process. indeed, some bacteria and viruses have been identified in atherosclerotic lesions: chlamydia pneumoniae, helicobacter pylori, streptococcus sanguis, porphyromonas gingivalis, herpes simplex viruses type 1 and 2, coxsakievirus b and hepatitis virus a. by their ability to induce antigenic and functional changes in the cells of the vascular wall (endothelial cells, smooth muscle cells, monocytes/macrophages, and ...200111776694
[cloning and analyzing of the conserved protein gene in streptococcus sanguinis atcc10556].to clone and analyze the conserved protein gene in streptococcus sanguinis(s.s) atcc10556.200111774210
[effects of streptococcus sanguis on blood composition, blood pressure and cardiac dysfunction in rabbis].to investigate effect of streptococcus sanguis on blood cell and cardiopulmonary changes.200111769649
bactericidal effect of delmopinol on attached and planktonic streptococcus sanguinis cells.the aim of this investigation was to determine the antibacterial effect of varying concentrations of delmopinol-hcl on attached as well as on planktonic streptooccus sanguinis cells in vitro. in addition, a possible antiadhesive effect on attached micro-organisms was to be investigated. s. sanguinis cells were allowed to attach to glass surfaces. these as well as planktonic cells were exposed to delmopinol-hci in concentrations ranging from 0.2% to 0.00005% for 2 min. the percentage of vital bac ...200111767281
anti-fibrin antibody binding in valvular vegetations and kidney lesions during experimental endocarditis.in streptococcus sanguinis (sanguis) induced experimental endocarditis, we sought evidence that the development of aortic valvular vegetation depends on the availability of fibrin. endocarditis was induced in new zealand white rabbits by catheter placement into the left ventricle and inoculation of the bacteria. fibrin was localized in the developing vegetation with 99mtechnetium (tc)-labeled anti-fibrin antibody one or three days later. when rabbit anti-fibrin antibody was given intravenously o ...200111762752
association of early onset periodontitis microbiota with aspartate aminotransferase activity in gingival crevicular fluid.the objective of this study was to determine the relationship between the activity of the enzyme aspartate aminotransferase (ast) in gingival crevicular fluid (gcf) using the colorimetric periogard (ptm) test and the subgingival microflora in early onset periodontitis lesions.200111737506
dysregulation of monocyte oxidative burst in streptococcal endocarditis.streptococcal subacute endocarditis is characterized by low-grade systemic inflammation. although structural cardiac defects are pivotal, phagocytic cells, i.e. monocytes and neutrophils, are involved in the induction and the course of bacterial endocarditis. decreased production of reactive oxygen metabolites was described in long-lasting infections. we hypothesized that the oxidative burst of phagocytes induced by the infecting organism is defective in patients with streptococcal endocarditis.200111737229
plaque formation on surface modified dental implants. an in vitro study.bacterial adhesion on titanium implant surfaces has a strong influence on healing and long-term outcome of dental implants. parameters like surface roughness and chemical composition of the implant surface were found to have a significant impact on plaque formation. the purpose of this study was to evaluate the influence of two physical hard coatings on bacterial adhesion in comparison with control surfaces of equivalent roughness. two members of the oral microflora, streptococcus mutans and str ...200111737097
co-adhesion and removal of adhering bacteria from salivary pellicles by three different modes of brushing.this study compares removal of pairs of co-adhering and non-co-adhering oral actinomyces and streptococci by hand, electric and sonic brushing from salivary pellicles. in addition, re-deposition of a co-adhering and non-co-adhering streptococcal strain to brushed pellicles was studied. first, actinomyces were allowed to adhere to a pellicle in a flow chamber, after which streptococci suspended in saliva were perfused through the chamber at 33 c. pellicles with adhering bacterial pairs were brush ...200111695753
proteolytic degradation of oral biofilms in vitro and in vivo: potential of proteases originating from euphausia superba for plaque control.this paper deals with enzymatic removal of dental plaque, in vitro as well as in vivo, using proteases from the antarctic krill shrimp (euphausia superba), referred to as krillase. krillase exhibits both endo- and exopeptidase activity but has no microbicidal effect. in model systems with pure cultures of oral microorganisms. krillase demonstrated inhibition of microbial adhesion to saliva-coated hydroxyapatite. furthermore, a protocol for the growth of reproducible in vitro plaque films has bee ...200111695752
plaque removal characteristics of electric toothbrushes using an in vitro plaque model.the purpose of the study was to investigate and quantify the efficacy of plaque removal by commercially available electric toothbrushes using an in vitro system based on artificial plaque grown on glass slides.200111686826
cariogenic potential of lactosylfructoside as determined by acidogenicity of oral streptococci in vitro and human dental plaque in situ.the cariogenic potential of lactosylfructoside [o-beta-d-galactopyranosyl-(1-->4)-o-alpha-d-glucopyranosyl-(1<-->2)-beta-d-fructofuranoside] was estimated by experiments on oral streptococci in vitro and human dental plaque in situ. lactosylfructoside was unable to support growth of the strains of streptococcus mutans and s. sobrinus used in this study. however, it was able to support growth of strains of s. sanguis, s. mitis and s. oralis. acid was produced rapidly by cell suspensions of s. ora ...200111641569
susceptibilities of oral bacteria and yeast to mammalian cathelicidins.the effects of cathelicidins against oral bacteria and clinically important oral yeasts are not known. we tested the susceptibilities of actinobacillus actinomycetemcomitans, fusobacterium nucleatum, porphyromonas gingivalis, streptococcus sanguis, candida krusei, candida tropicalis and candida albicans to the following cathelicidins: fall39, smap29, and cap18. smap29 and cap18 were antimicrobial, whereas fall39 did not exhibit antimicrobial activity. future studies are needed to determine the p ...200111600383
in vitro antimicrobial activities of bakuchiol against oral microorganisms.bakuchiol was isolated from the seeds of psoralea corylifolia, a tree native to china with various uses in traditional medicine, followed by extraction with ether and column chromatography combined with silica gel and octyldecyl silane. in this study, the antimicrobial activities of bakuchiol against some oral microorganisms were evaluated in vitro. the cell growth of streptococcus mutans was inhibited in a bakuchiol concentration-dependent manner, and growth of s. mutans was completely prevente ...200111600349
phosphorylcholine-dependent cross-reactivity between dental plaque bacteria and oxidized low-density lipoproteins.antibodies reactive with phosphorylcholine (pc) are ubiquitous in human sera, but the antigens stimulating their production and their function are not clear. previous studies have shown that a significant proportion of dental plaque bacteria contain pc as determined by reactivity with pc-specific mouse myeloma proteins and monoclonal antibodies. additionally, serum antibody concentrations of immunoglobulin (igg) g anti-pc are higher in sera of individuals who have experienced periodontal attachm ...200111598029
actinomyces naeslundii fimbrial protein orf977 shows similarity to a streptococcal adhesin.the nucleotide sequence of the chromosomal dna, upstream of actinomyces naeslundii t14v fimbrial gene fima, was determined. one open reading frame (orf977) encoding 977 amino acids was found, preceded by a gene homologous to elongation factor tu. database searches revealed that orf977 was homologous to csha, a streptococcus gordonii protein involved in cell adhesion. previous studies had already determined two genes in the type 2 fimbrial gene cluster of a. naeslundii t14v: the structural subuni ...200111555311
alteration of the coadherence of candida albicans with oral bacteria by dietary sugars.interactions between bacterial oral flora and candida albicans are important in denture plaque formation. this study therefore first aimed to quantify the coadherence of c. albicans and bacteria by the use of a bioluminescent adenosine triphosphate (atp) assay based on the firefly luciferase-luciferin system. the second aim was to examine the effect of i) dietary sugars (used for preculture) and ii) enzymatic digestion of fungi on the coadherence. when yeast was preincubated in yeast nitrogen ba ...200111555304
the ability of direct fluorescence-based, two-colour assays to detect different physiological states of oral streptococci.to investigate the ability of six fluorescent-based, two-colour viability assays to detect different physiological growth stages of two oral streptococci species.200111555201
adherence of streptococcus sanguis.the presence of adhesins on the cell surface of s. sanguis enables the organism to grow and survive in the oral cavity. many workers are now actively involved in attempting to characterise these adhesins and the molecular basis for adherence of various streptococci. the complexity of the adhesion processes is underlined by the many varied opinions as to the nature of the molecular components and biochemical interactions that are involved. understanding the molecular mechanisms involved in bacter ...198611547805
antibacterial activity of hydrastis canadensis extract and its major isolated alkaloids.the antibacterial activity of extract and isolated major alkaloids (berberine, beta-hydrastine, canadine and canadaline) of hydrastis canadensis l. (ranunculaceae) was evaluated against 6 strains of microorganism: staphylococcus aureus (atcc 25 993 and atcc 6538p), streptococcus sanguis (atcc 10 556), escherichia coli (atcc 25 922), pseudomonas aeruginosa (atcc 27 853). bactericidal activity was evaluated by contact test by measuring the "killing time" on a low density bacterial inoculum, and ba ...200111509983
bacterial detachment from salivary conditioning films by dentifrice supernates.this study compared the detachment by supernates of nine different dentifrices of four oral bacterial strains adhering to a salivary pellicle in a parallel plate flow chamber. ultra-thin bovine enamel slabs were coated for 1.5 h with human whole saliva. following buffer rinsing, a bacterial suspension of streptococcus oralis, streptococcus sanguis, streptococcus mutans or actinomyces naeslundii was perfused through the flow chamber at a shear rate of 30 s-1 for four hours, and the number of adhe ...200211507932
enterococcus faecalis--a mechanism for its role in endodontic failure.the aim of this study was to identify a possible mechanism that would explain how e. faecalis could survive and grow within dentinal tubules and reinfect an obturated root canal.200111482724
do viridans streptococci cause pneumonia in children? 200111465856
endocarditis caused by penicillin-resistant viridans streptococci: 2 cases and controversies in therapy.although penicillin-resistant viridans streptococci have been isolated from samples from the mouth, blood, and wounds in increasing numbers, viridans streptococci isolated from patients with endocarditis have remained sensitive to penicillin for the past 5 decades. we report the cases of 2 patients with penicillin-resistant viridans streptococcal endocarditis, review 6 other cases from the literature, and summarize 2 studies that used an animal model of penicillin-resistant viridans streptococca ...200111462199
phenotypic and genotypic diversity of streptococcus sanguis in infants.streptococcus sanguis comprises a heterogeneous group of oral streptococci indigenous to the oral cavity of humans. a total of 289 isolates from an infant population (n=37) were tentatively identified as s. sanguis on the basis of the distinctive colony morphology as shown on mm10-sucrose non-selective medium. these isolates were divided into four biovars of s. sanguis as determined by an extended panel of biochemical attributes. chromosomal dna was extracted from each isolate, and an ap-pcr fin ...200111442849
inhibitory effects of moabs against a surface protein antigen in real-time adherence in vitro and recolonization in vivo of streptococcus mutans.a surface protein antigen (pac) of streptococcus mutans, particularly the a-region of the molecule, has been reported to interact with salivary components on the tooth surface. it might be a candidate antigen inducing the production of antibodies against the adherence of s. mutans to the tooth surface. we investigated the effects of monoclonal antibodies (moabs) obtained by immunization of synthetic pac peptides that completely correspond to the amino acid sequence of part of the a-region. these ...200111439156
investigation of bacteremia after orthodontic banding and debanding following chlorhexidine mouth wash application.this study investigates the prevalence of bacteremia after orthodontic banding and debanding, following the application of a 0.2% chlorhexidine gluconate mouthwash. the banding and debanding groups were each composed of 40 young adult patients. in the banding group, patients were asked to rinse their mouth with chlorhexidine gluconate for 60 seconds just prior to fitting of the bands. in the debanding group, they were asked to use the mouthwash immediately before removal of bands and brackets. i ...200111407771
the effect of oral bacteria on candida albicans germ-tube formation.a total of eight bacterial isolates belonging to six species, and a select group of 12 oral candida albicans isolates, were used to study the effect of bacteria on germ-tube formation. briefly, each bacterial suspension (10(5-6) cells/ml) was mixed with a c. albicans suspension (10(7) cells/ml) and incubated at 37 degrees c for 90 min with bovine serum, and the percentage germ-tube-positive candida cells was quantified using a haemocytometer, under light microscopy. in general, out of eight bact ...200111398996
predominant cultivable subgingival microbiota of healthy and hiv-infected ethnic chinese.although clinically distinctive types of periodontal disease are known to be associated with hiv infection, the pathogenesis remains unclear. in the present study, the subgingival microflora of 21 hiv-infected and 11 hiv-free ethnic chinese were studied using the direct microscopic and anaerobic culture methods. motile curved rods were found to be three times higher in the hiv-infected group under direct microscopy. otherwise, there were no significant differences between the diseased and health ...200111398993
antibacterial activity of particulate bioglass against supra- and subgingival bacteria.particulate bioglass is a bioactive material used in the repair of periodontal defects. this material undergoes a series of surface reactions in an aqueous environment which lead to osseointegration. the aim of this study was to determine whether these reactions exerted an antibacterial effect on a range of oral bacteria. streptococcus sanguis, streptococcus mutans and actinomyces viscosus were suspended in nutrient broth (nb), artificial saliva (as) or dulbecco's modified eagle medium plus 10% ...200111374470
amine and tin fluoride inhibition of streptococcus sanguis adhesion under continuous flow.this study evaluated the ability of topically applied amine fluoride (amf) and amf-tin fluoride to inhibit the adhesion of streptococcus sanguis within a parallel plate flow cell system. one of three amf compounds and two tin fluoride preparations significantly reduced the net bacterial adhesion to conditioned glass over a 1-h period. tin(iv) fluoride inhibited s. sanguis adhesion to the greatest extent, and this was shown to be dependent on the formation of the conditioning film prior to agent ...200111358541
are dental infections a cause of brain abscess? case report and review of the literature.dental pathology and/or treatment have been linked to a small number of brain abscesses as possible sources of infection. a further case is presented, in which a dental site is implicated. a review of the evidence was undertaken. a wide range of dental procedures had been implicated. in some cases the brain isolate was not of dental origin. in many, the diagnosis was one of exclusion. in order to confirm the role of odontogenic infection in the pathogenesis of brain abscess, modern sampling tech ...200111354924
a case-control study of respiratory disease in thoroughbred racehorses in sydney, australia.in order to investigate the role of infectious agents in the aetiology of lower respiratory tract disease in thoroughbred racehorses, a matched case-control study was conducted. cases were identified by the presence of coughing, and were compared to a control population matched on time of sample collection and location within the same training establishment. tracheal wash samples were collected from 100 cases and 148 controls. case horses were more likely than controls to have endoscopic and cyt ...200111352347
quinolone-resistance mechanisms and in vitro susceptibility patterns among european isolates of streptococcus mitis, streptococcus sanguis, and streptococcus pneumoniae. 200111347679
recommended conservation of the names streptococcus sanguis, streptococcus rattus, streptococcus cricetus, and seven other names included in the approved lists of bacterial names. request for an opinion.with reference to the first principle of the international code of nomenclature of bacteria, which emphasizes stability of names, it is proposed that the original names streptococcus sanguis, streptococcus rattus, streptococcus cricetus, erwinia ananas, eubacterium tarantellus, lactobacillus sake, nitrosococcus oceanus, pseudomonas betle, rickettsia canada and streptomyces rangoon, all included in the approved lists of bacterial names, be conserved. request for an opinion.200111321121
effects of three different infant dentifrices on biofilms and oral microorganisms.the purpose of this work was to evaluate the effects of infant dentifrices: a--with lactoperoxidase, glucose oxidase and lactoferrin; b--with 1100 ppm of naf and sodium lauryl sulfate; c--with extract of calendula. the dentifrices were test on biofilms formed in vitro from saliva and dental plaque of infants, using reference strains a. viscosus (atcc 43146); c. albicans (atcc 51501); l. casei (atcc 4646); s. mitis (atcc 49456); s. mutans (atcc 25175); s. oralis (atcc 35037); s. sanguis (atcc 105 ...200011314149
investigation on interaction between streptococcus sanguis and porphyromonas gingivalis in specific pathogen-free rats.to examine whether endogenous streptococcus can prevent or reduce the colonization of the virulent porphyromonas gingivalis strain.200011314089
interleukin-10 regulates the tissue factor activity of monocytes in an in vitro model of bacterial endocarditis.monocytes are important effector cells in the pathogenesis of bacterial endocarditis since they provide the tissue factor that activates the coagulation system and maintains established vegetations. monocytes secrete cytokines that can modulate monocyte tissue factor activity (tfa), thereby affecting the formation and maintenance of vegetations. in this study, we show that monocytes cultured for 4 h on a streptococcus sanguis-infected fibrin matrix mimicking the in vivo vegetational surface expr ...200111292741
monocytes augment bacterial species- and strain-dependent induction of tissue factor activity in bacterium-infected human vascular endothelial cells.in bacterial endocarditis (be), intravascular infection with staphylococcus aureus, streptococcus sanguis, or staphylococcus epidermidis can lead to formation of a fibrin clot on the inner surface of the heart and cause heart dysfunction. the events that start the coagulation in the early stage of the disease are largely unknown. we have recently shown that human endothelial cells (ec) upon binding and internalization of s. aureus, but not s. sanguis or s. epidermidis, express tissue factor (tf) ...200111292691
oral colonization and cariogenicity of streptococcus gordonii in specific pathogen-free tan:spfom(om)br rats consuming starch or sucrose diets.the significance of streptococcus gordonii in dental caries is undefined, as is that of other alpha-amylase-binding bacteria (abb) commonly found in the mouth. to clarify the ecological and cariological roles of s. gordonii our specific pathogen-free osborne-mendel rats, tan:spfom(om)br, were fed either diet 2000 (containing 56% confectioner's sugar, most of which is sucrose) or diet 2000cs (containing 56% cornstarch, in lieu of confectioner's sugar) and inoculated with s. gordonii strains. unin ...200111269866
ph-regulated secretion of a glyceraldehyde-3-phosphate dehydrogenase from streptococcus gordonii fss2: purification, characterization, and cloning of the gene encoding this enzyme.streptococcus gordonii and other viridans streptococci (vs) are primary etiologic agents of infective endocarditis, despite being part of the normal oral microflora. recently, a surface-bound glyceraldehyde-3-phosphate dehydrogenase (gapdh) has been found on the cells of all tested streptococcal species, where it has been implicated as a virulence factor. in contrast, we observed that a soluble extracellular gapdh was the major secreted protein from s. gordonii fss2, an endocarditis strain. the ...200111269731
binding of oral streptococci to human fibrinogen.the interaction of oral streptococci with human fibrinogen was investigated. streptococcus gordonii was chosen as a representative species to study the binding to fibrinogen. s. gordonii dl1 adhered to immobilized fibrinogen and bovine serum albumin. binding to immobilized fibrinogen was saturable, concentration and temperature dependent. the binding of s. gordonii dl1 to fibrinogen was inhibited by anti-fibrinogen antibody. heating of the bacteria for 1 h at 95 degrees c resulted in 90% inhibit ...200111240861
mannosidase production by viridans group streptococci.the production of mannosidase activity by all currently recognized species of human viridans group streptococci was determined using an assay in which bacterial growth was dependent on the degradation of the high-mannose-type glycans of rnase b and subsequent utilization of released mannose. rnase b is an excellent substrate for the demonstration of mannosidase activity since it is a glycoprotein with a single glycosylation site which is occupied by high-mannose-type glycoforms containing five t ...200111230417
addition of antibacterial agents to mma-tbb dentin bonding systems--influence on tensile bond strength and antibacterial effect.to produce a bonding system which has both high bond strength and antibacterial properties, an antibacterial agent (vancomycin: vcm or metronidazol: mn) was added to the pmma powder of 4-meta/mma-tbb resin (cb). the influence of the addition of an antibacterial agent on tensile bond strength to dentin and the antibacterial effect were investigated in this study. forty-seven freshly extracted bovine first or second incisors were used to measure the tensile bond strength to dentin. the bond streng ...200011219091
comparative evaluation of antibacterial effects of nd:yag laser irradiation in root canals and dentinal tubules.the antibacterial effects of the nd:yag laser on contaminated root canals and dentinal tubules were observed as the aim of this study. the samples were inoculated with streptococcus sanguis (nctc 7853) and prevotella intermedia (nctc 93336), and the effects of nd:yag laser were tested on these teeth. the specimens were lased with 1.8 w and 2.4 w nd:yag laser for 30 s, and the presence of bacteria in tubules was observed under light microscopy. the 1.8 w laser sterilized the tubules in 86.3% of s ...200011199732
in vitro study of the penetration of streptococcus sanguis and prevotella intermedia strains into human dentinal tubules.the persistent presence of bacteria in the root canal system often leads to the failure of treatment. the purpose of this study was to determine in vitro penetration of streptococcus sanguis and prevotella intermedia into dentinal tubules. samples obtained from human teeth were inoculated with a strain of s. sanguis (nctc 7853) and p. intermedia (nctc 93336) for 20 days. bacterial penetration into tubules was investigated at scanning electron microscopy and light microscopic level. the results s ...200011199726
[effects of sodium doecyl sulfate on the artificial dental plaque in chemostat].sodium doecyl sulfate (sds) is widely used as a detergent in dentifrices. it has been shown to interfere with the protein adsorption to hydroxyapatite (ha), and inhibit acquired pellicle formation. the aim of the present study was to examine the effects of sds on the artificial dental plaque in chemostat. the amount of the 3h-labelled bacteria adhered on the enamel fragment surface was determined with scintillometer. the artificial dental plaque was observed under the scanning electron microscop ...199711189307
[the effect of chlorhexidine varnish system on streptococcus mutans in fissure plaques].a chlorhexidine varnish system and a controlled releasing varnish were prepared by the authors. the release rate of chlorhexidine in vitro and the effects on s. mutans and s. sanguis in vivo were determined. the results showed that the system could selectively inhibit the growth of s. mutans in fissure plaque and had no effect on s. sanguis. the period of interaction between the layer of varnish and plaque was prolonged by the control-releasing reagent. the study suggests that the chlorhexidine ...199711189283
utilization and acid production of beta-galactosyllactose by oral streptococci and human dental plaque.beta-galactosyllactose is a trisaccharide containing the beta-galactosidic linkage at the nonreducing end. the purpose of this study was to determine whether certain oral streptococci could utilize four kinds of beta-galactosyllactoses. three of four beta-galactosyllactoses were unable to support growth of the oral streptococci and to be a substrate for producing acid from the cell suspensions and dental plaque. 4'-beta-galactosyllactose supported growth of streptococcus sanguis atcc 35105, atcc ...200111169141
post-ph effect in oral streptococci.to study the post-ph effect (pphe) in 11 strains of oral streptococci belonging to streptococcus sanguis, s. mitis, s. gordonii, s. mutans and s. sobrinus by using the bact/alert microbial detection system.200011168090
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