Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| highly efficient chemoenzymatic synthesis of beta1-4-linked galactosides with promiscuous bacterial beta1-4-galactosyltransferases. | two bacterial beta1-4-galactosyltransferases, nmlgtb and hp1-4galt, exhibit promiscuous and complementary acceptor substrate specificity. they have been used in an efficient one-pot multienzyme system to synthesize lacnac, lactose, and their derivatives including those containing negatively charged 6-o-sulfated glcnac and c2-substituted glcnac or glc, from monosaccharide derivatives and inexpensive glc-1-p. | 2010 | 20625591 |
| characterization of the ptfa gene of avian pasteurella multocida strains by allele-specific polymerase chain reaction. | pasteurella multocida is the causative agent of fowl cholera in domesticated and wild birds. the disease outcome is affected by various host- and pathogen-specific determinants. several putative virulence factors have been proposed to play a key role in this interaction, including the ptfa gene, the products of which assemble to form type 4 fimbriae on the bacterial surface. one way to understand more precisely how ptfa contributes to pathogenesis is to gather molecular features of this gene in ... | 2010 | 20622234 |
| bacterial pathogens of the bovine respiratory disease complex. | pneumonia caused by the bacterial pathogens discussed in this article is the most significant cause of morbidity and mortality of the brdc. most of these infectious bacteria are not capable of inducing significant disease without the presence of other predisposing environmental factors, physiologic stressors, or concurrent infections. mannheimia haemolytica is the most common and serious of these bacterial agents and is therefore also the most highly characterized. there are other important bact ... | 2010 | 20619191 |
| peritonitis associated with pasteurella multocida: molecular evidence of zoonotic etiology. | a patient on continuous cyclic peritoneal dialysis for chronic kidney disease due to type 2 diabetes mellitus developed peritoneal dialysis-associated peritonitis induced by pasteurella multocida that was isolated from a sample of dialysis effluent. the route of infection was unknown for this case; however, p. multocida was also isolated from a culture of a pharyngeal swab obtained from the patient's cat. there was no evidence that the cat had bitten and ruptured the peritoneal dialysis tubing o ... | 2010 | 20609195 |
| the occurrence of bordetella bronchiseptica in pigs with clinical respiratory disease. | between january 2003 and september 2008, 652 bordetella bronchiseptica isolates were cultured from 3506 lung samples collected from pigs with respiratory disease. over the 6-year period, the average isolation rate was 18.6%, making b. bronchiseptica the fourth most frequently isolated pathogenic bacterium from those lung samples. the isolation rates in different years and provinces ranged from 15.2% to 25.7% and 17.3% to 20.7%, respectively. there were significant influences of sampling month an ... | 2011 | 20598597 |
| natural selection in the chicken host identifies 3-deoxy-d-manno-octulosonic acid kinase residues essential for phosphorylation of pasteurella multocida lipopolysaccharide. | pasteurella multocida is the causative agent of a number of diseases in animals, including fowl cholera. p. multocida strains simultaneously express two lipopolysaccharide (lps) glycoforms (glycoforms a and b) that differ only in their inner core structure. glycoform a contains a single 3-deoxy-d-manno-octulosonic acid (kdo) residue that is phosphorylated by the kdo kinase, kdka, whereas glycoform b contains two unphosphorylated kdo residues. we have previously shown that p. multocida mutants la ... | 2010 | 20566690 |
| [usefulness and limit of gram staining smear examination]. | gram staining is one of the most simple and inexpensive methods for the rapid diagnosis of bacterial and fungal infections. it yields results much faster than culture, and provides important data for the patient's treatment and prognosis. however, a difference exists in the quality and quantity of information yielded by gram staining smears based on the experience and knowledge of those conducting the tests. therefore, a risk of misdiagnosis based on the information obtained from gram staining s ... | 2010 | 20560458 |
| pasteurella multocida thymidine kinase 1 efficiently activates pyrimidine nucleoside analogs. | in the pasteurella multocida genome only one putative deoxyribonucleoside kinase encoding gene, for thymidine kinase 1 (pmtk1), was identified. the pmtk1 gene was sub-cloned into escherichia coli ky895 and it sensitized the host towards 2',2'-difluoro-deoxycytidine (gemcitabine, dfdc), 3'-azido-thymidine (azt) and 5-fluoro-deoxyuridine (5f-du). pmtk1 was over-expressed and purified with two different tags. apparently, deoxyuridine (du), and not thymidine (dt), is the preferred substrate. we sugg ... | 2010 | 20544520 |
| characterization of the membrane-targeting c1 domain in pasteurella multocida toxin. | pasteurella multocida toxin (pmt) is a virulence factor responsible for the pathogenesis of some forms of pasteurellosis. the toxin activates g(q)- and g(12/13)-dependent pathways through the deamidation of a glutamine residue in the alpha-subunit of heterotrimeric gtpases. we recently reported the crystal structure of the c terminus (residues 575-1285) of pmt (c-pmt), which is composed of three domains (c1, c2, and c3), and that the c1 domain is involved in the localization of c-pmt to the plas ... | 2010 | 20534589 |
| [cross-protective effect of the cp39 adhesive proteins against avian pasteurella multicida in mice]. | we evaluated the cross-protective effect of a 39-kda adhesive protein (cp39), recombinant adhesive protein (rcp39), and capsular proteins from in vitro and in vivo grown avian p. multocida in mice. | 2010 | 20499641 |
| pasteurella multocida bacteremia in humans: a clinical report. | although pasteurella multocida is a very common pathogen in animals, it has rarely been recognized as an infection of man. the first human infection was reported in 1913; between then and 1960 only 40 cases were reported in the world literature. the present patient became toxically ill following a bite from a domestic cat, and pasteurella multocida was grown on blood culture.the reason for the study is that the infecting agent can cause lesions in almost any body tissue, but up till now no repor ... | 1974 | 20469060 |
| pets or pest: peritoneal dialysis-related peritonitis due to pasteurella multocida. | pasteurella multocida is a gram-negative bacteria found in the oropharynx of many domestic animals. p. multocida can cause a variety of human infections, but it remains a rare cause of peritoneal dialysis-related peritonitis. we describe a severe case of peritoneal dialysis-related peritonitis due to p. multocida infection caused by close contact with a cat. | 2010 | 20457433 |
| comparison of genotypic and phenotypic characterization methods for pasteurella multocida isolates from fatal cases of bovine respiratory disease. | bovine respiratory disease (brd) is the most costly disease of beef cattle in north america. because pasteurella multocida is a commensal of the upper respiratory tract, it is generally considered an opportunistic pathogen. however, studies in swine indicated that there may be a limited number of strains associated with disease, suggesting that some are more virulent than others. although this may also be true of isolates from cattle, appropriate typing methods must be established before this po ... | 2010 | 20453209 |
| chemo-enzymatic synthesis of the carbohydrate antigen n-glycolylneuraminic acid from glucose. | n-glycolylneuraminic acid (neu5gc) is a non-human sialic acid, which may play a significant role in human pathologies, such as cancer and vascular disease. further studies into the role of neu5gc in human disease are hindered by limited sources of this carbohydrate. using a chemo-enzymatic approach, neu5gc was accessed in six steps from glucose. the synthesis allows access to gram-scale quantities quickly and economically and produces neu5gc in superior quality to commercial sources. finally, we ... | 2010 | 20452577 |
| cytokine profiles, apoptosis and pathology of experimental pasteurella multocida serotype a1 infection in mice. | mice were experimentally infected with pasteurella multocida serotype a1 to study the cytokine profiles, host cell apoptosis and sequential pathology at different hours of post-infection. infected mice were dull, anorectic and depressed. a transient leukocytopenia followed by progressive leukocytosis was observed in the course of infection. serum cytokine profiles showed significantly (p<0.01) higher amount of pro-inflammatory cytokines (tnf-α, il-1β, il-6 and mouse kc) in the infected mice when ... | 2010 | 20447665 |
| influence of pasteurella multocida infection on the pharmacokinetic behavior of marbofloxacin after intravenous and intramuscular administrations in rabbits. | the pharmacokinetic behavior of marbofloxacin was studied in healthy (n = 12) and pasteurella multocida infected rabbits (n = 12) after single intravenous (i.v.) and intramuscular (i.m.) administrations. six rabbits in each group (control and diseased) were given a single dose of 2 mg/kg body weight (bw) of marbofloxacin intravenously. the other six rabbits in each group were given the same dose of the drug intramuscularly. the concentration of marbofloxacin in plasma was determined using high-p ... | 2010 | 20444027 |
| bacteremia, sepsis, and peritonitis with pasteurella multocida in a peritoneal dialysis patient. | 2010 | 20424206 | |
| pharmacodynamics of amoxicillin against mannheimia haemolytica and pasteurella multocida and pharmacokinetic/pharmacodynamic (pk/pd) correlation in sheep. | silicone-made tissue cages were implanted in sheep. blood serum (sbs) and tissue cage fluid (tcf) samples were collected after amoxicillin intravenous and intramuscular administrations, at the dose of 15 mg/kg. amoxicillin pharmacodynamics were studied in an artificial culture medium, sbs and tcf with use of a mannheimia haemolytica and a pasteurella multocida strain. a concentration-independent antimicrobial activity of amoxicillin was confirmed for levels higher than 0.79-1.75×mic. this result ... | 2010 | 20363488 |
| analysis of the biosynthesis genes and chemical components of the capsule of avibacterium paragallinarum. | the aim of this study was to investigate biosynthesis genes and chemical components of the capsule of avibacterium paragallinarum. the sequence of a 10-kb region containing the capsule biosynthetic locus of av. paragallinarum was determined. two reference strains, i.e., 221 (serovar a) and h18 (serovar c), together with four taiwanese field strains (all serovar c) were sequenced. the results showed that there are two genotypes (i and ii) of the capsule biosynthetic locus in av. paragallinarum, a ... | 2010 | 20362405 |
| protective potential of an attenuated pasteurella multocida, which expresses only the n-terminal truncated fragment of p. multocida toxin. | pasteurella multocida serogroup d causes progressive atrophic rhinitis in pigs and produces a potent, intracellular, mitogenic toxin known as p. multocida toxin (pmt), which is encoded by the toxa gene. highly toxic to cells, pmt is a poor antigen and becomes more immunogenic after its native structure has been destroyed. previously, we found that the n-terminal fragment of pmt (n-pmt) can induce a strong immune response that is protective against wild-type challenge. here, an attenuated p. mult ... | 2010 | 20357954 |
| [construction and characterization of recombinant salmonella enterica serovar choleraesuis vaccine strain expressing heterologous antigen of pasteurella multocida toxin]. | this study was aimed to construct and characterize salmonella vaccine strain c500 expressing the recombinant pasteurella multocida toxin (pmt) antigen by the asd+ balanced-lethal host-vector system. | 2010 | 20344946 |
| pasteurella multocida toxin is a potent activator of anti-apoptotic signalling pathways. | toxigenic pasteurella multocida strains produce a 146 kda protein toxin (pmt) that due to its high mitogenic activity is thought to possess carcinogenic properties. pmt affects several signal transduction pathways related to cancer by constitutively stimulating heterotrimeric g proteins. downstream of galpha(q), galpha(13) and galpha(i), the toxin activates the small gtpase rhoa, map kinases and signal transducer and activator of transcription (stat) proteins. pmt also stimulates gbetagamma sign ... | 2010 | 20331638 |
| unusual pathogenicity of pasteurella multocida isolated from the throats of common wild rats. | 1947 | 20268506 | |
| identification of a conserved membrane localization domain within numerous large bacterial protein toxins. | vibrio cholerae is the causative agent of the diarrheal disease cholera. many virulence factors contribute to intestinal colonization and disease including the multifunctional autoprocessing rtx toxin (martx(vc)). the rho-inactivation domain (rid) of martx(vc) is responsible for inactivating the rho-family of small gtpases, which leads to depolymerization of the actin cytoskeleton. based on a deletion analysis of rid to determine the minimal functional domain, we have identified a subdomain at t ... | 2010 | 20212166 |
| outer membrane proteins of pasteurella multocida. | pasteurella multocida is a ubiquitous pathogen which causes a range of diseases in diverse animal species. components of the bacterial outer membrane, such as trans membrane proteins and lipoproteins, play key roles in the interaction of the pathogen with the host environment and in the host immune response to infection. in this review, we evaluate the current knowledge of p. multocida outer membrane proteins and their role in pathogenesis and immunity. | 2010 | 20197220 |
| an investigation of the pathology and pathogens associated with porcine respiratory disease complex in denmark. | respiratory infections are among the most important diseases of growing pigs. in order to elucidate the multifactorial aetiology of porcine respiratory disease complex (prdc) in denmark, lungs from 148 finishing pigs with cranioventral bronchopneumonia (case group) and 60 pigs without lung lesions (control group) were collected from abattoirs. the pathogens involved in prdc and their interactions were identified and linked to the histopathological diagnosis. the lung samples were cultured for ba ... | 2010 | 20181357 |
| occurrence of mycoplasma hyopneumoniae infections in suckling and nursery pigs in a region of high pig density. | the occurrence of mycoplasma hyopneumoniae infections in young pigs was surveyed in a retrospective study of 1122 datasets obtained from routine diagnostics where either suckling or nursery pigs were examined for m hyopneumoniae in lung tissue. findings were correlated with the presence of lung lesions, detection of other respiratory pathogens, vaccination history and parameters describing the herd of origin. the prevalence of m hyopneumoniae in lung tissue from 201 suckling pigs was 2.0 per cen ... | 2010 | 20154309 |
| fis is essential for capsule production in pasteurella multocida and regulates expression of other important virulence factors. | p. multocida is the causative agent of a wide range of diseases of animals, including fowl cholera in poultry and wild birds. fowl cholera isolates of p. multocida generally express a capsular polysaccharide composed of hyaluronic acid. there have been reports of spontaneous capsule loss in p. multocida, but the mechanism by which this occurs has not been determined. in this study, we identified three independent strains that had spontaneously lost the ability to produce capsular polysaccharide. ... | 2010 | 20140235 |
| molecular classification and its impact on diagnostics and understanding the phylogeny and epidemiology of selected members of pasteurellaceae of veterinary importance. | in this paper we briefly report recent taxonomic changes that have taken place within the bacterial family pasteurellaceae as a result of molecular investigations. we address the complexity and diagnostic possibilities and outline the current knowledge on the population structure these investigations have generated. in addition, we discuss future possibilities to improve basic understanding of genetic diversity at population level, possible host adaptation and stability of clones associated with ... | 2010 | 20135907 |
| development of a multi-locus sequence typing scheme for avian isolates of pasteurella multocida. | a total of 63 isolates of pasteurella multocida from australian poultry, all associated with fowl cholera outbreaks, and three international reference strains, representing the three subspecies within p. multocida were used to develop a multi-locus sequence typing scheme. primers were designed for conserved regions of seven house-keeping enzymes -adk, est, gdh, mdh, pgi, pmi and zwf - and internal fragments of 570-784 bp were sequenced for all isolates and strains. the number of alleles at the d ... | 2010 | 20129742 |
| kikuchi disease in association with pasteurella multocida infection. | kikuchi disease is a rare condition characterized by fever and persistent lymphadenopathy. its aetiology is unknown, but it has been associated with viral, bacterial, and protozoal agents. we report the case of a 15-year old boy who grew pasteurella multocida from blood cultures with kikuchi disease confirmed by histopathologic lymph node examination. | 2010 | 20123771 |
| pasteurella multocida infection in the neonates. | 2010 | 20118754 | |
| bovine pasteurellosis and other bacterial infections of the respiratory tract. | despite technological, biologic, and pharmacologic advances the bacterial component of the bovine respiratory disease (brd) complex continues to have a major adverse effect on the health and wellbeing of stocker and feeder cattle. overlooked in this disappointing assessment is evaluation of the effects that working with younger, lighter-weight cattle have on managing the bacterial component of the brd complex. most problems associated with brd come from cattle taken from and comingled with cattl ... | 2010 | 20117542 |
| acute infection of a total knee arthroplasty caused by pasteurella multocida: a case report and a comprehensive review of the literature in the last 10 years. | total knee arthroplasty (tka) infection are most commonly due staphylococcus aureus followed by coagulase-negative staphylococci, and streptococci, while gram-negative rods are seldom isolated.(1,3,4) in the last 20 years, cases of pasteurella multocida tka and total hip arthroplasty (tha) infection resulting from cat and dog bites, scratches, or licks have been published reporting varying presentations and treatment options. most commonly, p. multocida infected arthroplasties result in local te ... | 2010 | 20116315 |
| haemophilus influenzae clinical isolates with plasmid pb1000 bearing blarob-1: fitness cost and interspecies dissemination. | plasmid pb1000 is a mobilizable replicon bearing the bla(rob-1) beta-lactamase gene that we have recently described in haemophilus parasuis and pasteurella multocida animal isolates. here we report the presence of pb1000 and a derivative plasmid, pb1000', in four haemophilus influenzae clinical isolates of human origin. pulsed-field gel electrophoresis showed unrelated patterns in all strains, indicating that the existence of pb1000 in h. influenzae isolates is not the consequence of clonal diss ... | 2010 | 20086141 |
| [cellulitis after a cat bite]. | animal bite wounds are common. domestic companion animals inflict the majority of these wounds. the most important percentage of contagions are due to cat bites, and often by pasteurella species. we present two cases of pasteurella multocida infection after a cat bite.thus, in this article we review the most relevant clinical features related with this aetiological agent and some aspects about antimicrobial susceptibility. | 2009 | 20082044 |
| two concomitant but unrelated cases of pasteurella multocida infection, including meningitis secondary to pituitary adenoma microsurgery. | 2010 | 20079991 | |
| experimental iron-inactivated pasteurella multocida a: 1 vaccine adjuvanted with bacterial dna is safe and protects chickens from fowl cholera. | fowl cholera is a serious problem in large and small scale poultry production. the present study describes the development and testing of an inactivated whole-cell, low-cost, safe, and effective vaccine for fowl cholera based on a previous work (vaccine 23:5590-5598). pasteurella multocida a: 1 grown in the presence of low fecl(3) concentrations, inactivated with higher concentrations of fecl(3), and adjuvanted with bacterial dna from p. multocida b: 2 containing immunostimulatory cpg motifs pro ... | 2010 | 20074684 |
| immunomodulatory activity of methanolic extract of morus alba linn. (mulberry) leaves. | the leaves of morus alba linn. (family: moraceae) commonly known as mulberry are mainly used as food for the silkworms and they are sometimes eaten as vegetable or used as cattle fodder in different parts of the world. the effect of morus alba on the immune system was evaluated by using different experimental models such as carbon clearance test, cyclophosphamide induced neutropenia, neutrophil adhesion test, effect on serum immunoglobulins, mice lethality test and indirect haemagglutination tes ... | 2010 | 20067869 |
| bibersteinia trehalosi inhibits the growth of mannheimia haemolytica by a proximity-dependent mechanism. | mannheimia (pasteurella) haemolytica is the only pathogen that consistently causes severe bronchopneumonia and rapid death of bighorn sheep (bhs; ovis canadensis) under experimental conditions. paradoxically, bibersteinia (pasteurella) trehalosi and pasteurella multocida have been isolated from bhs pneumonic lungs much more frequently than m. haemolytica. these observations suggest that there may be an interaction between these bacteria, and we hypothesized that b. trehalosi overgrows or otherwi ... | 2010 | 20038698 |
| occurrence of a pig respiratory disease associated with swine influenza a (h1n2) virus in tochigi prefecture, japan. | in february 2008, a feeder pig herd of the affected farm in tochigi prefecture, japan, showed increasing respiratory symptoms; by april, the situation worsened with 12-16 pigs dying daily. diagnostic tests revealed the presence of h1n2 subtype of swine influenza virus (siv) and pasteurella multocida from nasal swab and lung emulsion. serological tests by hemagglutination inhibition method and enzyme-linked immunosorbent assay method (elisa; imported from u.s.a.) indicated the spread of siv into ... | 2010 | 20035121 |
| pasteurella multocida tracheobronchitis in a patient with cll on rituximab. | 2010 | 20033893 | |
| update on bacterial pathogenesis in brd. | mannheimia haemolytica, pasteurella multocida, histophilus somni, mycoplasma bovis and arcanobacterium pyogenes are all frequently implicated in bovine respiratory disease (brd). m. haemolytica is considered the most important of the group. these bacteria are commensals in the nasopharynx and establish infection in the lungs of cattle that are subjected to a variety of stresses. factors that permit adherence to and proliferation in the lungs and factors that cause tissue destruction and inflamma ... | 2009 | 20003651 |
| bovine respiratory disease research (1983-2009). | bovine respiratory disease (brd) research has provided significant understanding of the disease over the past 26 years. modern research tools that have been used include monoclonal antibodies, genomics, polymerase chain reaction, immunohistochemistry (ihc), dna vaccines and viral vectors coding for immunogens. emerging/reemerging viruses and new antigenic strains of viruses and bacteria have been identified. methods of detection and the role for cattle persistently infected bovine viral diarrhea ... | 2009 | 20003649 |
| pasteurella multocida meningitis caused by kissing animals: a case report and review of the literature. | 2010 | 19997925 | |
| expression and immunoprotective property of a 39-kda plpb protein of pasteurella multocida. | pasteurella multocida is a gram-negative bacterium known to infect a wide range of domestic and wild animals. the increasing incidence of p. multocida isolated from cases of fowl cholera and hemorrhagic septicemia has led to a renewed interest in this pathogen as well as in the development of vaccines. in this study, pcr primers were designed to amplify the fragment of plpb gene from p. multocida fc-pakchong (a:1). the purified pcr product of plpb gene consisting of 831 base pairs was inserted i ... | 2009 | 19959899 |
| characterization and time course of pulmonary lesions in calves after intratracheal infection with pasteurella multocida a:3. | pasteurella multocida a:3 is a common cause of suppurative bronchopneumonia in calves and results in significant production losses and mortality. here we describe the lesions in three calves at each of four time points (1 day and 4, 7 and 10 days) after experimental intratracheal infection with approximately 1x10(9) colony-forming units of p. multocida a:3 moredun research institute (mri isolate 671/90). equivalent age- and time-matched sham-dosed negative control animals were also studied. infe ... | 2010 | 19951821 |
| mhc haplotype and susceptibility to experimental infections (salmonella enteritidis, pasteurella multocida or ascaridia galli) in a commercial and an indigenous chicken breed. | in three independent experimental infection studies, the susceptibility and course of infection of three pathogens considered of importance in most poultry production systems, ascaridia galli, salmonella enteritidis and pasteurella multocida were compared in two chicken breeds, the indigenous vietnamese ri and the commercial luong phuong. furthermore, the association of the major histocompatibility complex (mhc) with disease-related parameters was evaluated, using alleles of the lei0258 microsat ... | 2010 | 19945754 |
| characterisation of ceftiofur resistance in swine bacterial pathogens. | a pcr based method was developed for the identification of ceftiofur resistance genes (bla(cmy-2), bla(tem-1), and ampc) in swine bacterial pathogens. using this method, the ceftiofur resistant (n=76) and susceptible (n=45) strains of bordetella bronchiseptica, salmonella spp., escherichia coli, and pasteurella multocida were screened for the presence of these three genes. the resistant genes were detected in 70% (bla(tem-1)), 68% (bla(cmy-2)) and 45% (ampc) of the resistant isolates and in 18% ... | 2011 | 19914850 |
| a study on the severity and relevance of porcine circovirus type 2 infections in dutch fattening pigs with respiratory diseases. | this study was set up to get more insights in the severity and relevance of porcine circovirus type 2 (pcv2) infections in dutch fattening farms in an endemic pcv2-situation with no clinical signs of post-weaning multisystemic wasting syndrome (pmws). in part a of the study, in total 29 commercial fattening farms with varying percentages of pneumonia and pleurisy at slaughter were examined. blood samples were collected at random by cross-sectional sampling; 10 in the age of 10-12 weeks, 10 at th ... | 2010 | 19913367 |
| an outbreak of avian cholera in wild waterfowl in ordos wetland, inner mongolia, china. | eleven species of wild waterfowl (anseriformes and charadriiformes) were found dead in the hongjian nur lake in the ordos wetland, inner mongolia of northern china, in 2007. pasteurella multocida was isolated from tissue samples of dead and sick birds and identified as p. multocida subsp. multocida, serotype a1, using serologic and molecular techniques. eight bird species in this outbreak had never been previously reported with p. m. multocida infection. this was also the first report of fowl ch ... | 2009 | 19901396 |
| phenotypic and genetic characterization of pasteurella multocida and related isolates from rabbits in switzerland. | several bacteria belonging to the family pasteurellaceae are potential pathogens in rabbits. in particular, pasteurella multocida is considered to be important, and outbreaks caused by this species result in considerable economic losses in rabbitries. however, pasteurellaceae spp. isolated from rabbits are poorly characterized, and thus, proper identification of p. multocida isolates from these animals is problematic and often unsatisfactory, thereby hampering epidemiological investigations. the ... | 2009 | 19901279 |
| acute phase protein changes in calves during an outbreak of respiratory disease caused by bovine respiratory syncytial virus. | bovine acute phase proteins (apps), lipopolysaccharide binding protein (lbp), serum amyloid a (saa), haptoglobin (hp) and alpha(1)-acid glycoprotein (agp) were evaluated as inflammatory markers during an outbreak of bovine respiratory disease (brd) caused by bovine respiratory syncytial virus (brsv). calves (n = 10) presented mild to moderate signs of respiratory disease. secondary bacterial infections, pasteurella multocida and mycoplasma dispar as major species, were detected in tracheobronchi ... | 2011 | 19897247 |
| single-chain urokinase in empyema induced by pasturella multocida. | intrapleural fibrin deposition and subsequent fibrosis characterize evolving empyema and contribute to the morbidity associated with this condition. single-chain urokinase (scupa) is proenzyme form of the urokinase plasminogen activator, which has recently been shown to effectively clear intrapleural loculation in tetracycline-induced pleurodesis in rabbits. the authors therefore hypothesized that scupa could likewise improve intrapleural injury associated with empyema. the authors used a rabbit ... | 2009 | 19895321 |
| trans-sialidase activity of photobacterium damsela alpha2,6-sialyltransferase and its application in the synthesis of sialosides. | trans-sialidases catalyze the transfer of a sialic acid from one sialoside to an acceptor to form a new sialoside. alpha2,3-trans-sialidase activity was initially discovered in the parasitic protozoan trypanosoma cruzi, and more recently was found in a multifunctional pasteurella multocida sialyltransferase pmst1. alpha2,8-trans-sialidase activity was also described for a multifunctional campylobacter jejuni sialyltransferase cstii. we report here the discovery of the alpha2,6-trans-sialidase ac ... | 2010 | 19880425 |
| [a case of cat-scratch-induced pasteurella multocida infection presenting with disseminated intravascular coagulation and acute renal failure]. | domestic animals are the main reservoirs of pasteurella species for human zoonosis due to bites and scratches. pasterurella multocida may cause serious soft-tissue infection and, less commonly, sepsis or septic shock, particularly in insufficient initial therapy and an immunocompromised host. we report a case of cat-scratch-induced p. multocida infection, presenting with disseminated intravascular coagulation and acute renal failure. a febrile 83-year-old woman with consciousness disturbance and ... | 2009 | 19860260 |
| identification and characterization of a novel antibacterial peptide, avian beta-defensin 2 from ducks. | in this study, a novel avian beta-defensin (avbd) was isolated from duck pancreas. the complete nucleotide sequence of the gene contained an 195 bp open reading frame encoding 64 amino acids. homology, characterization and comparison of the gene with avbd from other avian species confirmed that it was duck avbd2. the mrna expression of the gene was analyzed in 17 tissues from 21-day-old ducks. avbd2 was highly expressed in the trachea, crop, heart, bone marrow, and pancreas; moderately expressed ... | 2009 | 19851734 |
| pleuritis in slaughter pigs: relations between lung lesions and bacteriology in 10 herds with high pleuritis. | pleuritis in slaughter pigs has increased in recent years in the netherlands. the aim of the present study was to determine what respiratory pathogens were involved in pleuritis. in total, lungs of 968 slaughter pigs from 10 herds with high prevalence of pleuritis were morphologically examined for size, location, and type of lesions. moreover, histology and bacteriology were performed. examination of gross lung lesions showed 45% pleuritis, 14% pleuropneumonia and 38% catarrhal pneumonia. peribr ... | 2010 | 19836811 |
| expression of 4 truncated fragments of pasteurella multocida toxin and their immunogenicity. | pasteurella multocida toxin (pmt) is a poor antigen that becomes more immunogenic after its native structure has been destroyed. in contrast, partially truncated pmt proteins, which are predicted to be good antigens when used as a vaccine, might be used to improve the control of atrophic rhinitis in pigs. in this study, 4 truncated pmt fragments were expressed in escherichia coli, and those 4 fragments were inoculated into mice to produce the polyclonal antibodies. the results of an enzyme-linke ... | 2009 | 19794890 |
| immune responses to haemorrhagic septicaemia (hs) vaccination in trypanosoma evansi infected buffalo-calves. | to assess the immunosuppressive effect of trypanosoma evansi infection in buffalo-calves on immune responses to heterologous antigen, the study was planned to examine the responses of haemorrhagic septicaemia vaccination in simultaneously and previously (80 days before vaccination) t. evansi-infected buffalo-calves. eight buffalo-calves were divided into three groups. buffalo-calves of group a (n = 3) were previously (80 days before primary vaccination with haemorrhagic septicaemia [hs] vaccine) ... | 2010 | 19784863 |
| structural and genetic basis for the serological differentiation of pasteurella multocida heddleston serotypes 2 and 5. | pasteurella multocida is classified into 16 serotypes according to the heddleston typing scheme. as part of a comprehensive study to define the structural and genetic basis of this scheme, we have determined the structure of the lipopolysaccharide (lps) produced by p. multocida strains m1404 (b:2) and p1702 (e:5), the type strains for serotypes 2 and 5, respectively. the only difference between the lps structures made by these two strains was the absence of a phosphoethanolamine (petn) moiety at ... | 2009 | 19767423 |
| in vitro synthesis of heparosan using recombinant pasteurella multocida heparosan synthase pmhs2. | in vertebrates and bacteria, heparosan the precursor of heparin is synthesized by glycosyltransferases via the stepwise addition of udp-n-acetylglucosamine and udp-glucuronic acid. as heparin-like molecules represent a great interest in the pharmaceutical area, the cryptic pasteurella multocida heparosan synthase pmhs2 found to catalyze heparosan synthesis using substrate analogs has been studied. in this paper, we report an efficient way to purify pmhs2 and to maintain its activity stable durin ... | 2010 | 19756580 |
| what is your diagnosis? | 2009 | 19751160 | |
| characterization of pasteurellaceae-like bacteria isolated from clinically affected psittacine birds. | the aim of the present investigation was to identify and characterize pasteurella-like isolates obtained from clinically affected psittacine birds. | 2010 | 19732214 |
| pasteurella multocida meningitis in newborns after incidental animal exposure. | we present briefly 2 newborns with pasteurella multocida meningitis following incidental household exposure to domestic pets and review 36 additional cases from the literature. of 38 reported cases of p. multocida meningitis during infancy, 18 were less than or equal to 1 month of age. all but one of the infants less than 1 month of age had documented exposure to household dogs and/or cats, most only incidentally. | 2009 | 19730153 |
| abdominal granuloma in a young healthy man after infection with pasteurella multocida. | 2009 | 19727097 | |
| pasteurella multocida infection, a rare cause of mycotic abdominal aortic aneurysm. | a 64-year-old man was admitted with abdominal pain 6 weeks after treatment with intravenous flucloxacillin for cellulitis of his right leg. urgent operation was necessary for a mycotic aneurysm of the abdominal aorta due to infection with pasteurella multocida, a microorganism residing in the oral cavity of domestic animals that very rarely causes infection of native arteries or grafts. the aorta was repaired with a rifampin-coated tube graft. despite postoperative duodenal perforation, abdomina ... | 2009 | 19703750 |
| management of human and animal bite wound infection: an overview. | animal and human bite wounds can lead to serious infections. the organisms recovered generally originate from the biter's oral cavity and the victim's skin flora. anaerobes were isolated from more than two thirds of human and animal bite infections. streptococcus pyogenes is often recovered in human bites, pasteurella multocida in animal bites, eikenella corrodens in animal and human, capnocytophaga spp, neisseria weaveri, weeksella zoohelcum, neisseria canis, staphylococcus intermedius, nonoxid ... | 2009 | 19698283 |
| new plasmid tools for genetic analysis of actinobacillus pleuropneumoniae and other pasteurellaceae. | we have generated a set of plasmids, based on the mobilizable shuttle vector pmidg100, which can be used as tools for genetic manipulation of actinobacillus pleuropneumoniae and other members of the pasteurellaceae. a tandem reporter plasmid, pmc-tandem, carrying promoterless xyle and gfpmut3 genes downstream of a multiple-cloning site (mcs), can be used for identification of transcriptional regulators and conditions which favor gene expression from different cloned promoters. the ability to det ... | 2009 | 19666733 |
| a fatal case of pasteurella multocida epiglottitis. | pasteurella multocida, a gram-negative bacillus, is rarely associated with acute respiratory infections. | 2009 | 19662492 |
| [pasteurella multocida septic oligoarthritis]. | 2010 | 19640663 | |
| antimicrobial susceptibility of pasteurella multocida isolated from swine and poultry. | pasteurella multocida causes infectious diseases in a wide range of animal species. antimicrobial therapy is still an effective tool for treatment. generally, p. multocida isolates are susceptible to most of the widely used commercial antimicrobial agents but their excessive and unjustified use accelerates the emergence of resistant strains. we defined the antimicrobial sensitivity pattern of 56 p. multocida strains isolated from poultry (20) and swine [16 p. multocida toxin (pmt) positive and 2 ... | 2009 | 19635708 |
| [pasteurella multocida septic shock]. | 2009 | 19635259 | |
| protection against fowl cholera conferred by vaccination with recombinant pasteurella multocida filamentous hemagglutinin peptides. | three gene fragments, derived from pasteurella multocida strain p-1059 (serotype a:3), encoding approximately the 5' one-third of fhab2 were overexpressed individually in escherichia coli. the recombinant peptides were purified, pooled, and administered to turkey poults to evaluate immunity. the results showed that turkeys immunized twice with the recombinant peptides were significantly protected against intranasal challenge with p. multocida strain p-1059. vaccination elicited antibody response ... | 2009 | 19630220 |
| protease activated receptor signaling is required for african trypanosome traversal of human brain microvascular endothelial cells. | using human brain microvascular endothelial cells (hbmecs) as an in vitro model for how african trypanosomes cross the human blood-brain barrier (bbb) we recently reported that the parasites cross the bbb by generating calcium activation signals in hbmecs through the activity of parasite cysteine proteases, particularly cathepsin l (brucipain). in the current study, we examined the possible role of a class of protease stimulated hbmec g protein coupled receptors (gpcrs) known as protease activat ... | 2009 | 19621073 |
| the transcriptional response of pasteurella multocida to three classes of antibiotics. | pasteurella multocida is a gram-negative bacterial pathogen that has a broad host range. one of the diseases it causes is fowl cholera in poultry. the availability of the genome sequence of avian p. multocida isolate pm70 enables the application of functional genomics for observing global gene expression in response to a given stimulus. we studied the effects of three classes of antibiotics on the p. multocida transcriptome using custom oligonucleotide microarrays from nimblegen systems. hybridi ... | 2009 | 19607655 |
| atypical non-progressive pneumonia in goats. | an outbreak of severe respiratory disease in a goat herd was associated with mycoplasma ovipneumoniae, mycoplasma arginini, mannheimia haemolytica and pasteurella multocida with mortality rates exceeding 20% in kids. post mortem features in affected kids included severe pleuropneumonia, lung consolidation, large quantities of pleural fluid and pericarditis. this is the first report of atypical proliferative pneumonia in goats in portugal. | 2010 | 19596209 |
| lung pathology and infectious agents in fatal feedlot pneumonias and relationship with mortality, disease onset, and treatments. | this study charted 237 fatal cases of bovine respiratory disease (brd) observed from may 2002 to may 2003 in a single oklahoma feed yard. postmortem lung samples were used for agent identification and histopathology. late in the study, 94 skin samples (ear notches) were tested for bovine viral diarrhea virus (bvdv) by immunohistochemistry (ihc). bovine respiratory disease morbidity was 14.7%, and the mortality rate of all causes was 1.3%, with more than half (53.8%) attributed to brd (0.7% total ... | 2009 | 19564494 |
| bite-related and septic syndromes caused by cats and dogs. | bite infections can contain a mix of anaerobes and aerobes from the patient's skin and the animal's oral cavity, including species of pasteurella, streptococcus, fusobacterium, and capnocytophaga. domestic cat and dog bite wounds can produce substantial morbidity and often require specialised care techniques and specific antibiotic therapy. bite wounds can be complicated by sepsis. disseminated infections, particularly those caused by capnocytophaga canimorsus and pasteurella multocida, can lead ... | 2009 | 19555903 |
| multiresistance in pasteurella multocida is mediated by coexistence of small plasmids. | in most gram-negative bacteria, acquired multiresistance is conferred by large plasmids compiling numerous antimicrobial resistance genes. here, we show an evolutionary alternative strategy used by pasteurella multocida to become resistant to multiple clinically relevant antibiotics. thirteen beta-lactam-resistant clinical isolates, concomitantly resistant to tetracyclines and/or streptomycin as well as to sulfonamides, were studied. pulsed-field gel electrophoresis analysis revealed different p ... | 2009 | 19528282 |
| [overwhelming postsplenectomy infection 22 years after splenectomy]. | a 25-year-old man undergoing splenectomy at 3 years of age to treat idiopathic thrombocytopenic purpura but no history of streptococcus pneumonia vaccination, and reporting high fever, nausea, and headache developed purpura, confusion, and hypotension the next day and was admitted. detailed examination showed disseminated intravascular coagulation and multiple-organ dysfunction. chest x-ray and computed tomography (ct) showed pneumonia and pleural effusion. blood culture was positive for s. pneu ... | 2009 | 19522311 |
| [penicillin v a first choice in infected cat bites]. | 2009 | 19496253 | |
| genetic diversity of porcine pasteurella multocida strains from the respiratory tract of healthy and diseased swine. | a total of 382 porcine pasteurella multocida strains, isolated from cases of pneumonia and progressive atrophic rhinitis (par) as well as from clinically healthy pigs of more than 150 german husbandries were characterized by detection of virulence-associated genes (vags) and ribotyping to understand the relationships between "commensal" and "pathogenic" strains, enabling a rational choice of vaccine strains. the diversity of the strains according to vags was low and mainly limited to capsular ty ... | 2009 | 19487087 |
| development and application of a reverse transcriptase polymerase chain reaction to detect chinese isolates of duck hepatitis virus type 1. | we developed a reverse transcriptase polymerase chain reaction (rt-pcr) method for the detection of duck hepatitis virus type 1 (dhv-1) in the tissues of infected and clinically affected ducks and in chick and duck embryos. we found the assay to be effective in detecting the virus in china, where it is being used in studies on the epidemiology of the disease. we applied this simple and rapid diagnostic method to the detection of dhv isolates grown in chick and duck embryos and in tissues obtaine ... | 2009 | 19475729 |
| development and evaluation of an antigen-capture elisa for detection of the ul24 antigen of the duck enteritis virus, based on a polyclonal antibody against the ul24 expression protein. | an antigen-capture enzyme-linked immunosorbent assay (ac-elisa) method was developed for the efficient detection of the ul24 antigen of the duck enteritis virus (dev) using polyclonal antibodies. ducks and rabbits were immunized, respectively, with expressed ul24 recombinant protein. the igg antibodies against ul24 from ducks and rabbits were purified and used as the capture antibodies. the specificity of the optimized ac-elisa was evaluated by use of dev, duck hepatitis virus (dhv), duck hepati ... | 2009 | 19467266 |
| [septic arthritis and osteomyelitis caused by pasteurella multocida: a new case]. | 2009 | 19457335 | |
| molecular diversity of porcine and human isolates of pasteurella multocida. | to examine the variability among pasteurella multocida strains isolated from pigs (nasal, tonsil and lung specimens) and humans in france. | 2009 | 19457034 |
| a retrospective six-year national survey of p. multocida infections in israel. | pasteurella multocida is the commonest organism infecting pet bites. anecdotal reports tend to overemphasize dramatic outcomes. we aimed to study a large database of p. multocida infections. this retrospective survey of p. multocida infections in israeli hospitals refers to the y 2000-2005. clinical microbiologists were contacted by email and asked to perform a back-search of their hospital's records for isolates of p. multocida. the charts of patients growing p. multocida were abstracted into a ... | 2009 | 19452350 |
| antimicrobial susceptibility and resistance of chicken escherichia coli, salmonella spp., and pasteurella multocida isolates. | escherichia coli, salmonella species, and pasteurella multocida are the major bacterial pathogens isolated from poultry. difference in susceptibility to antibiotics by microorganisms has become a major factor in drug choice and success of treatment. great concerns have been raised regarding emerging antimicrobial resistance among bacteria that may result in unpredictable antimicrobial susceptibility and failure of therapy. the primary objective of the present study was to determine the levels of ... | 2009 | 19432009 |
| efficacy of a novel pasteurella multocida vaccine against progressive atrophic rhinitis of swine. | the efficacy of a novel vaccine composed of three short recombinant subunit pasteurella multocida toxin (pmt) proteins in combination with a bi-valent p. multocida whole-cell bacterin (rspmt-pm) was evaluated in field studies for prevention and control of progressive atrophic rhinitis (par) of swine at 15 conventional farrow-to-finish farms. experimental piglets that were immunized twice with the rspmt-pm vaccine developed detectable titers of neutralizing antibodies (greater than 1:8) that prev ... | 2009 | 19428902 |
| rapid virulence typing of pasteurella multocida by multiplex pcr. | the gram-negative bacterium pasteurella multocida constitutes a heterogeneous species associated with wide range of disease in many animals. isolates are classified into five groups based on capsular antigen (capa, b, d, e and f). recently, a new valuable pcr-based method was introduced to determine the epidemiological correlation between p. multocida infection and existence of virulence genes including tbpa, pfha, toxa and hgbb. however, this method is tedious and laborious. thus, in the curren ... | 2009 | 19414187 |
| rapidly progressive and lethal septicemia due to infection with pasteurella multocida in an infant. | we report a case of rapidly progressive pneumonia and meningoencephalitis in a 2-month-old breastfed infant. culture of cerebrospinal fluid was positive for pasteurella multocida. no bite or scratch injury as site of entrance could be detected clinically or at autopsy, therefore aerogenic transmission in droplets from a household pet must be considered. infections with p. multocida have been frequently reported in infants and elderly people; however, rapidly progressive and lethal infections in ... | 2009 | 19412752 |
| [pasteurella multocida meningitis: report of 2 cases]. | 2009 | 19403203 | |
| pasteurella multocida toxin activation of heterotrimeric g proteins by deamidation. | pasteurella multocida toxin is a major virulence factor of pasteurella multocida, which causes pasteurellosis in men and animals and atrophic rhinitis in rabbits and pigs. the approximately 145 kda protein toxin stimulates various signal transduction pathways by activating heterotrimeric g proteins of the galpha(q), galpha(i), and galpha(12/13) families by using an as yet unknown mechanism. here, we show that pasteurella multocida toxin deamidates glutamine-205 of galpha(i2) to glutamic acid. th ... | 2009 | 19369209 |
| pasteurella multocida infection in a cirrhotic patient: case report, microbiological aspects and a review of literature. | pasteurellosis is a zoonosis often caused by cat or dog bites or scratches, or by direct exposure to their secretions. pasteurella multocida is the main pathogen involved in infections through domestic animal bites; generally a local infection characterized by its particular virulence with consequent rapid onset. serious infection has also been reported in persons affected by comorbidity without domestic animal bite injuries. here we report the case of a woman with lower limb exudating vesicular ... | 2009 | 19366651 |
| sialic acid uptake is necessary for virulence of pasteurella multocida in turkeys. | many pathogenic bacteria employ systems to incorporate sialic acid into their membranes as a means of protection against host defense mechanisms. in pasteurella multocida, an opportunistic pathogen which causes diseases of economic importance in a wide range of animal species, sialic acid uptake plays a role in a mouse model of systemic pasteurellosis. to further investigate the importance of sialic acid uptake in pathogenesis, sialic acid uptake mutants of an avian strain of p. multocida p-1059 ... | 2009 | 19366625 |
| [bordetella dermonecrotic toxin and pasteurella multocida toxin causing turbinate atrophy in pigs]. | 2009 | 19348254 | |
| alternative method for genetic transformation of pasteurella multocida x73 using a hyaluronidase-producing staphylococcus aureus strain. | the hyaluronic acid-rich capsules make affected bacteria relatively retractile to transformation. in this work, native hyaluronidase produced in situ by staphylococcus aureus, was investigated to replace the pure enzyme in bacterial capsule depolymerization and its subsequent genetic transformation. the encapsulated pasteurella multocida x73 was cultivated as a mixed culture with a hyaluronidase-producing strain of s. aureus. then different concentrations (50 ng and 200 ng) of the pasteurella-es ... | 2009 | 19345706 |
| costs of reproduction in a long-lived bird: large clutch size is associated with low survival in the presence of a highly virulent disease. | fitness costs of reproduction are expected to be more pronounced when the environmental conditions deteriorate. we took advantage of a natural experiment to investigate the costs of reproduction among common eiders (somateria mollissima) nesting at a site in the arctic, where an avian cholera epizootic appeared at different magnitudes. we tested the predictions that larger reproductive effort (clutch size) is associated with lower survival or breeding probability the following year, and that thi ... | 2009 | 19324661 |
| a recombinant e. coli bioprocess for hyaluronan synthesis. | an escherichia coli strain, jm109, was successfully engineered into an efficient hyaluronic acid (ha) producer by co-expressing the only known class-ii ha synthase from a gram-negative bacterium (pasteurella multocida) and uridine diphosphate-glucose dehydrogenase from e. coli k5 strain. the engineered strain produced about 0.5 g/l ha in shake flask culture and about 2.0-3.8 g/l in a fed-batch fermentation process in a 1-l bioreactor. the sharp increase in viscosity associated with ha accumulati ... | 2009 | 19308402 |