Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| a new model for non-typeable haemophilus influenzae middle ear infection in the junbo mutant mouse. | acute otitis media, inflammation of the middle ear, is the most common bacterial infection in children and, as a consequence, is the most common reason for antimicrobial prescription to this age group. there is currently no effective vaccine for the principal pathogen involved, non-typeable haemophilus influenzae (nthi). the most frequently used and widely accepted experimental animal model of middle ear infection is in chinchillas, but mice and gerbils have also been used. we have established a ... | 2016 | 26611891 |
| bacterial profile and antimicrobial susceptibility patterns of otitis media in ayder teaching and referral hospital, mekelle university, northern ethiopia. | middle ear infection is a common problem for both children and adults particularly in resource limited countries. nevertheless, in ethiopia and particularly in the study area, there is scarcity of recent data that indicate the magnitude of the problem. thus this study aimed to identify bacterial isolates and determine their drug susceptibility patterns from patients who had ear infection. cross sectional study was carried out on patients with ear infection and who visited the ear, nose and throa ... | 2015 | 26609503 |
| draft genome sequences of eight nontypeable haemophilus influenzae strains previously characterized using an electrophoretic typing scheme. | nontypeable haemophilus influenzae is an important cause of human disease. strains were selected for genome sequencing to represent the breadth of nontypeable strains within the species, as previously defined by the electrophoretic mobility of 16 metabolic enzymes. | 2015 | 26607889 |
| pediatric bacterial meningitis in french guiana. | controlling vaccine-preventable infectious diseases is a public health priority in french guiana but there is currently no epidemiological data on pediatric bacterial meningitis in this overseas department. our aim was to describe data related to pediatric bacterial meningitis in french guiana and compare it with that of metropolitan france. | 2016 | 26607229 |
| a rare case of ruptured infrarenal aortic aneurysm infected with haemophilus influenzae type b. | 2017 | 26600811 | |
| the sputum microbiome in chronic obstructive pulmonary disease exacerbations. | acute exacerbations of chronic obstructive pulmonary disease (copd) are thought to be associated with--and perhaps to mediate--accelerated loss of lung function in copd. although the application of culture-independent methods for detection of bacteria have shown copd to be associated with marked differences in the burden, diversity, and composition of the bronchial bacterial microbiome, few studies have examined the changes associated with community-acquired exacerbations of the disease. in a lo ... | 2015 | 26595736 |
| acute respiratory infection and bacteraemia as causes of non-malarial febrile illness in african children: a narrative review. | the replacement of "presumptive treatment for malaria" by "test before treat" strategies for the management of febrile illness is raising awareness of the importance of knowing more about the causes of illness in children who are suspected to have malaria but return a negative parasitological test. the most common cause of non-malarial febrile illness (nmfi) in african children is respiratory tract infection. whilst the bacterial causes of nmfi are well known, the increasing use of sensitive tec ... | 2015 | 26594615 |
| maturation of molybdoenzymes and its influence on the pathogenesis of non-typeable haemophilus influenzae. | mononuclear molybdenum enzymes of the dimethylsulfoxide (dmso) reductase family occur exclusively in prokaryotes, and a loss of some these enzymes has been linked to a loss of bacterial virulence in several cases. the moba protein catalyzes the final step in the synthesis of the molybdenum guanine dinucleotide (mgd) cofactor that is exclusive to enzymes of the dmso reductase family. moba has been proposed as a potential target for control of virulence since its inhibition would affect the activi ... | 2015 | 26594204 |
| the burden of bacteremia and invasive diseases in children aged less than five years with fever in italy. | invasive diseases (id) caused by streptococcus pneumoniae (s. pneumoniae), haemophilus influenzae (h. influenzae), and neisseria meningitidis are a major public health problem worldwide. comprehensive data on the burden of bacteremia and id in italy, including data based on molecular techniques, are needed. | 2015 | 26589787 |
| changes in airway inflammation during pulmonary exacerbations in patients with cystic fibrosis and primary ciliary dyskinesia. | lung disease in patients with both primary ciliary dyskinesia (pcd) or cystic fibrosis (cf) is associated with impaired mucociliary clearance; however, clinical outcomes are typically worse in cf patients. we assessed whether cf and pcd patients differ in inflammatory response in the airways during pulmonary exacerbation.we first studied clinically stable pcd patients with a spectrum of bacterial pathogens to assess inflammatory response to different pathogens. subsequently, pcd and cf patients ... | 2016 | 26585432 |
| dual rna-seq of nontypeable haemophilus influenzae and host cell transcriptomes reveals novel insights into host-pathogen cross talk. | the ability to adhere and adapt to the human respiratory tract mucosa plays a pivotal role in the pathogenic lifestyle of nontypeable haemophilus influenzae (nthi). however, the temporal events associated with a successful colonization have not been fully characterized. in this study, by reconstituting the ciliated human bronchial epithelium in vitro, we monitored the global transcriptional changes in nthi and infected mucosal epithelium simultaneously for up to 72 h by dual rna sequencing. the ... | 2015 | 26578681 |
| a quasi-universal medium to break the aerobic/anaerobic bacterial culture dichotomy in clinical microbiology. | in the mid-19th century, the dichotomy between aerobic and anaerobic bacteria was introduced. nevertheless, the aerobic growth of strictly anaerobic bacterial species such as ruminococcus gnavus and fusobacterium necrophorum, in a culture medium containing antioxidants, was recently demonstrated. we tested aerobically the culture of 623 bacterial strains from 276 bacterial species including 82 strictly anaerobic, 154 facultative anaerobic, 31 aerobic and nine microaerophilic bacterial species as ... | 2016 | 26577141 |
| distinctive binding of avibactam to penicillin-binding proteins of gram-negative and gram-positive bacteria. | avibactam is a novel non-β-lactam β-lactamase inhibitor that covalently acylates a variety of β-lactamases, causing inhibition. although avibactam presents limited antibacterial activity, its acylation ability toward bacterial penicillin-binding proteins (pbps) was investigated. staphylococcus aureus was of particular interest due to the reported β-lactamase activity of pbp4. the binding of avibactam to pbps was measured by adding increasing concentrations to membrane preparations of a variety o ... | 2016 | 26574008 |
| nontypeable haemophilus influenzae exploits the interaction between protein-e and vitronectin for the adherence and invasion to bronchial epithelial cells. | nontypeable haemophilus influenzae (nthi) is one of the most common gram-negative pathogens in otitis media and exacerbation of chronic obstructive pulmonary disease. nthi has been reported to invade bronchial epithelial cells. this penetration enables nthi to evade the host immune system and antibiotics, and it seems to be related to the intractable features of these diseases. however, the precise mechanism of the invasion has been unknown. we hypothesized that protein-e, an outer membrane prot ... | 2015 | 26572616 |
| the epidemiology of invasive haemophilus influenzae non-serotype b disease in ontario, canada from 2004 to 2013. | since the widespread use of haemophilus influenzae (hi) type b (hib) vaccines among children aged <5 years, an increase in invasive non-hib disease incidence has been reported internationally. we sought to describe the epidemiology of invasive non-hib disease in ontario, canada (population ~13.5 million). | 2015 | 26569613 |
| clinical and molecular epidemiology of childhood invasive nontypeable haemophilus influenzae disease in england and wales. | in countries with established haemophilus influenzae type b (hib) immunization programs, nontypeable h. influenzae (nthi) is now responsible for nearly all invasive h. influenzae cases across all age groups. | 2016 | 26569188 |
| viral and bacterial etiology of severe acute respiratory illness among children < 5 years of age without influenza in niger. | globally, pneumonia is the leading cause of morbidity and mortality in children, with the highest burden experienced in sub-saharan africa and asia. however, there is a dearth of information on the etiology of severe acute respiratory illness (sari) in africa, including niger. | 2015 | 26567015 |
| identification and characterization of novel small molecule inhibitors of the acetyltransferase activity of escherichia coli n-acetylglucosamine-1-phosphate-uridyltransferase/glucosamine-1-phosphate-acetyltransferase (glmu). | this study aims at identifying novel chemical scaffolds as inhibitors specific to the acetyltransferase domain of a bifunctional enzyme, escherichia coli glmu, involved in the cell wall biosynthesis of gram-negative organisms. a two-pronged approach was used to screen a 50,000 small-molecule library. using the first approach, the library was in silico screened by docking the library against acetyltransferase domain of e. coli glmu studies. in the second approach, complete library was screened ag ... | 2016 | 26563552 |
| [a 1-year-old girl in whom nontypeable haemophilus influenzae was isolated from her blood after completion of h. influenzae type b vaccine--importance of serotyping of invasive of h. influenzae strain]. | 2015 | 26554227 | |
| neisseria meningitidis group a igg1 and igg2 subclass immune response in african children aged 12-23 months following meningococcal vaccination. | a group a meningococcal conjugate vaccine, psa-tt, was licensed in 2010 and was previously studied in a phase 2 clinical trial to evaluate its safety and immunogenicity in african children 12-23 months of age. | 2015 | 26553689 |
| accumulation of regulatory t cells and chronic inflammation in the middle ear in a mouse model of chronic otitis media with effusion induced by combined eustachian tube blockage and nontypeable haemophilus influenzae infection. | nontypeable haemophilus influenzae (nthi) is associated with chronic otitis media (com). in this study, we generated a murine model of com by using eustachian tube (et) obstruction and nthi (10(7) cfu) inoculation into the tympanic bulla, and we investigated the relationship between regulatory t cells (treg) and chronic inflammation in the middle ear. middle ear effusions (mees) and middle ear mucosae (mem) were collected at days 3 and 14 and at 1 and 2 months after inoculation. untreated mice s ... | 2015 | 26553466 |
| [analysis of non-serotype b encapsulated haemophilus influenzae isolated from pediatric patients]. | we analyzed non-serotype b encapsulated haemophilus influenzae (non-b hi) isolated from pediatric patients at chiba children's hospital during 2000-2012. among 3,532 clinical isolates of h. influenzae, there were 57 (1.6%) strains of non-b hi, 152 (4.3%) of serotype b h. influenzae (hib), and 3,323 (94.1%) of non-typeable h. influenzae (nthi). fifty-seven strains of non-b hi were serotyped useing the slide agglutination test and pcr. twenty-nine strains were identified as type e (hie) and 28 as ... | 2015 | 26552120 |
| seasonal variation in the incidence of late-onset bleb-related infection after filtering surgery in japan: the japan glaucoma society survey of bleb-related infection report 3. | to investigate seasonal variations in the incidence of late-onset bleb-related infection after filtering surgery in japan and the relationship between season and other factors. | 2016 | 26550963 |
| twenty years of bacterial genome sequencing. | twenty years ago, the publication of the first bacterial genome sequence, from haemophilus influenzae, shook the world of bacteriology. in this timeline, we review the first two decades of bacterial genome sequencing, which have been marked by three revolutions: whole-genome shotgun sequencing, high-throughput sequencing and single-molecule long-read sequencing. we summarize the social history of sequencing and its impact on our understanding of the biology, diversity and evolution of bacteria, ... | 2015 | 26548914 |
| [nasopharyngeal hib carriage among healthy children attending daycare centers in yokohama after one year of a publicly funded vaccine program]. | yokohama city started a regular, free vaccine program for haemophilus influenzae type b (hib) from february of 2011. this study was completed to verify the effectiveness of the vaccine on the nasopharyngeal hib carriage among healthy children attending daycare centers in the isogo area. the research was conducted during the late spring (jun--jul) and fall (oct-nov) of 2012. there was a significant decrease in the hib carriage rate (spring 8.8%, fall 1.6%). during this period there was no increas ... | 2015 | 26548294 |
| persistence of non-typeable haemophilus influenzae in the pharynx of children with adenotonsillar hypertrophy after treatment with azithromycin. | this study was performed in children with adenotonsillar hypertrophy to evaluate the effect of azithromycin (azt) on the presence of nthi in monocyte/macrophages (cd14(+) cells) of adenoids/tonsils and the persistence of nthi after adenotonsillectomy. a total of 36 pediatric patients participated in the study: 20 children were treated with azt before adenotonsillectomy, and 16 children did not receive the antibiotic prior to surgery. nthi were identified by culture and pcr in swabs and tissue sa ... | 2016 | 26546328 |
| bronchiectasis diagnosed after renal transplantation: a retrospective multicenter study. | bronchiectasis is characterized by abnormal, permanent and irreversible dilatation of the bronchi, usually responsible for daily symptoms and frequent respiratory complications. many causes have been identified, but only limited data are available concerning the association between bronchiectasis and renal transplantation. | 2015 | 26545860 |
| bacterial infections following splenectomy for malignant and nonmalignant hematologic diseases. | splenectomy, while often necessary in otherwise healthy patients after major trauma, finds its primary indication for patients with underlying malignant or nonmalignant hematologic diseases. indications of splenectomy for hematologic diseases have been reducing in the last few years, due to improved diagnostic and therapeutic tools. in high-income countries, there is a clear decrease over calendar time in the incidence of all indication splenectomy except nonmalignant hematologic diseases. howev ... | 2015 | 26543526 |
| evaluation of the effectiveness of the 2008 postsplenectomy vaccination joint theater trauma system clinical practice guideline. | postsplenectomy vaccination (psv) in an austere environment to minimize overwhelming postsplenectomy infection is challenging. we evaluated the clinical impact of a march 2008 clinical practice guideline (cpg) dictating immediate psv at north american treaty organization role 3 medical treatment facilities and subsequent complications. | 2015 | 26540709 |
| haemophilus influenzae type f hijacks vitronectin using protein h to resist host innate immunity and adhere to pulmonary epithelial cells. | the incidence of invasive haemophilus influenzae type b (hib) disease has significantly decreased since the introduction of an efficient vaccine against hib. however, in contrast to hib, infections caused by h. influenzae serotype f (hif) are emerging. we recently did a whole genome sequencing of an invasive hif isolate, and reported that hif interacts with factor h by expressing protein h (ph). in this study, upon screening with various human complement regulators, we revealed that ph is also a ... | 2015 | 26538390 |
| haemophilus influenzae triggers autophagy in hep-2 cells. | the map-lc3 system regulates the intracellular formation of autophagy-associated vacuoles. these vacuoles contain the lc3 protein; thus it has been utilized as a marker to identify autophagosomes. the aim of our study was to investigate whether haemophilus influenzae strains and their supernatants could activate autophagy in human larynx carcinoma cell line (hep-2). we demonstrate that higher expression of the lc3b-ii protein was induced, particularly by nontypeable haemophilus influenzae (nthi) ... | 2016 | 26537814 |
| etiology of community acquired pneumonia among children in india: prospective, cohort study. | childhood community acquired pneumonia (cap) is a significant problem in developing countries, and confirmation of microbial etiology is important for individual, as well as public health. however, there is paucity of data from a large cohort, examining multiple biological specimens for diverse pathogens (bacteria and viruses). the community acquired pneumonia etiology study (capes) was designed to address this knowledge gap. | 2015 | 26528392 |
| [the clinical picture and specific microbiological features of acute otitis media]. | the objective of the present work was to study the spectrum of bacterial pathogenic agents responsible for the development of acute otitis media under present conditions and to elucidate the relationship between the pathogen species and the clinical course of the inflammatory process in the middle ear. a total of 60 patients of either sex at the age varying from 18 to 64 patients were available for the examination. all of them complained of ear pain, purulent discharge from the ears, hearing imp ... | 2015 | 26525323 |
| etiology and outcome of diffuse acute infectious bronchiolitis in adults. | the etiology and outcome of diffuse acute infectious bronchiolitis (daib) in adults is not well known. | 2015 | 26524622 |
| streptococcus pneumoniae and haemophilus influenzae in paediatric meningitis patients at goroka general hospital, papua new guinea: serotype distribution and antimicrobial susceptibility in the pre-vaccine era. | bacterial meningitis remains an important infection globally, with the greatest burden in children in low-income settings, including papua new guinea (png). we present serotype, antimicrobial susceptibility and outcome data from paediatric meningitis patients prior to introduction of haemophilus influenzae type b (hib) and pneumococcal conjugate vaccines (pcvs) in png, providing a baseline for evaluation of immunisation programs. | 2015 | 26521138 |
| haemophilus influenzae licb contributes to lung damage in an aged mice co-infection model. | phosphorylcholine (chop) decoration of lipopolysaccharides is an important virulence strategy adopted by haemophilus influenzae to establish a niche on the mucosal surface and to promote adherence to the host cells. the incorporation of chop on the lps surface involves the lic1 operon, which consists of the lica, licb, licc, and licd genes. among which, licb is a choline transporter gene required for acquisition of choline from environmental sources. in this study, we investigated the pathogenes ... | 2016 | 26521136 |
| evaluation of haemophilus influenzae type b carrier status among children 10 years after the introduction of hib vaccine in brazil. | the aim of the present study was to assess the prevalence of haemophilus influenzae type b (hib) nasopharyngeal (np) colonisation among healthy children where hib vaccination using a 3p+0 dosing schedule has been routinely administered for 10 years with sustained coverage (> 90%). np swabs were collected from 2,558 children who had received the hib vaccine, of whom 1,379 were 12-< 24 months (m) old and 1,179 were 48-< 60 m old. hi strains were identified by molecular methods. hi carriage prevale ... | 2015 | 26517654 |
| ceftaroline activity on certain respiratory tract and wound infection agents at the minimum inhibitory concentration level. | the aim of this study was to investigate the effectiveness of ceftaroline against agents frequently isolated from respiratory tract and wound infections. | 2015 | 26517483 |
| tem-1 and rob-1 presence and antimicrobial resistance in haemophilus influenzae strains, istanbul, turkey. | resistance of 235 haemophilus influenzae clinical isolates from istanbul medical faculty hospital, turkey were determined against 19 antibiotics by disc diffusion method, and minimum inhibitory concentrations (mics) of those found resistant to ampicillin, cefuroxim, chloramphenicol and meropenem were measured using e-test. ampicillin-resistant isolates producing beta-lactamase as demonstrated by a nitrocefin assay were analyzed for the presence of tem-1 and rob-1 genes by pcr. eleven percent of ... | 2015 | 26513928 |
| middle ear response of muc5ac and muc5b mucins to nontypeable haemophilus influenzae. | chronic otitis media with effusion is characterized by middle ear secretion of mucin glycoproteins, predominantly muc5b; muc5ac, the other secretory mucin studied frequently, has also been identified in the middle ear. emerging evidence suggests a dichotomous role for these mucins in innate immune responses. we hypothesized that muc5ac is an acute responder and muc5b is expressed at later time points, reflecting a chronic situation. | 2015 | 26512766 |
| naturally acquired hmw1- and hmw2-specific serum antibodies in adults and children mediate opsonophagocytic killing of nontypeable haemophilus influenzae. | the hmw1 and hmw2 proteins are highly immunogenic adhesins expressed by approximately 75% of nontypeable haemophilus influenzae (nthi) strains, and hmw1- and hmw2-specific antibodies can mediate opsonophagocytic killing of nthi. in this study, we assessed the ability of hmw1- and hmw2-specific antibodies in sera from healthy adults and convalescent-phase sera from children with nthi otitis media to mediate killing of homologous and heterologous nthi. the serum samples were examined pre- and post ... | 2016 | 26512048 |
| haemophilus influenzae: a potent perinatal pathogen disproportionately isolated from indigenous women and their neonates. | nontypeable haemophilus influenzae (nthi) bacteraemia in pregnant women is strongly associated with pregnancy loss and preterm delivery. however, the clinical significance of isolation of nthi from nonsterile sites is unknown. | 2016 | 26503452 |
| antibiotic resistance among ocular pathogens in the united states: five-year results from the antibiotic resistance monitoring in ocular microorganisms (armor) surveillance study. | the antibiotic resistance monitoring in ocular microorganisms (armor) study is the only ongoing nationwide antibiotic resistance surveillance program specific to ocular pathogens. | 2015 | 26502312 |
| an independent evaluation of a novel peptide mimetic, brilacidin (pmx30063), for ocular anti-infective. | brilacidin (bri), a novel defensin mimetic, was evaluated as an ocular anti-infective. | 2016 | 26501484 |
| a discrete role for fnr in the transcriptional response to moderate changes in oxygen by haemophilus influenzae rd kw20. | the survival by pathogenic bacteria within the specific conditions of an anatomical niche is critical for their persistence. these conditions include the combination of toxic chemicals, such as reactive oxygen (ros) and reactive nitrogen species (rns), with factors relevant to cell growth, such as oxygen. haemophilus influenzae senses oxygen levels largely through the redox state of the intracellular fumarate-nitrate global regulator (fnr). h. influenzae certainly encounters oxygen levels that f ... | 2016 | 26499095 |
| development of the nasopharyngeal microbiota in infants with cystic fibrosis. | cystic fibrosis (cf) is characterized by early structural lung disease caused by pulmonary infections. the nasopharynx of infants is a major ecological reservoir of potential respiratory pathogens. | 2016 | 26492486 |
| pharmacokinetics/pharmacodynamics of peptide deformylase inhibitor gsk1322322 against streptococcus pneumoniae, haemophilus influenzae, and staphylococcus aureus in rodent models of infection. | gsk1322322 is a novel inhibitor of peptide deformylase (pdf) with good in vitro activity against bacteria associated with community-acquired pneumonia and skin infections. we have characterized the in vivo pharmacodynamics (pd) of gsk1322322 in immunocompetent animal models of infection with streptococcus pneumoniae and haemophilus influenzae (mouse lung model) and with staphylococcus aureus (rat abscess model) and determined the pharmacokinetic (pk)/pd index that best correlates with efficacy a ... | 2015 | 26482300 |
| reduced middle ear infection with non-typeable haemophilus influenzae, but not streptococcus pneumoniae, after transition to 10-valent pneumococcal non-typeable h. influenzae protein d conjugate vaccine. | in october 2009, 7-valent pneumococcal conjugate vaccine (pcv7: prevenar(tm) pfizer) was replaced in the northern territory childhood vaccination schedule by 10-valent pneumococcal haemophilus influenzae protein d conjugate vaccine (phid-cv10; synflorix(™) glaxosmithkline vaccines). this analysis aims to determine whether the reduced prevalence of suppurative otitis media measured in the phid-cv10 era was associated with changes in nasopharyngeal (np) carriage and middle ear discharge (ed) micro ... | 2015 | 26482232 |
| distribution of major pathogens from sputum and bronchoalveolar lavage fluid in patients with noncystic fibrosis bronchiectasis: a systematic review. | noncystic fibrosis (non-cf) bronchiectasis remains as a common health problem in asia. pathogens' distribution in airways of patients with non-cf bronchiectasis is important for doctors to make right decision. | 2015 | 26481748 |
| the outcome of h. influenzae and s. pneumoniae inter-species interactions depends on ph, nutrient availability and growth phase. | haemophilus influenzae and streptococcus pneumoniae exist together as common commensals of the healthy human nasopharynx, but both are important aetiological agents of different diseases, including the paediatric disease otitis media. it was recently shown that the formation of a multispecies biofilm of h. influenzae and s. pneumoniae is the cause of chronic forms of otitis media. however, the interactions between the two species are not clearly defined. using a defined and kinetic analysis, our ... | 2015 | 26481153 |
| haemophilus influenzae isolates survive for up to 20 years at -70 °c in skim milk tryptone glucose glycerol broth (stggb) if thawing is avoided during re-culture. | haemophilus influenzae remains a major cause of disease worldwide requiring continued study. recently, isolates of streptococcus pneumoniae and moraxella catarrhalis, but not h. influenzae, were reported to survive long-term ultra-freeze storage in stggb. we show that nontypeable h. influenzae isolates survive for up to 20 years when thawing is avoided. | 2015 | 26477641 |
| coaggregation occurs between microorganisms isolated from different environments. | coaggregation, the specific recognition and adherence of different microbial species, is thought to enhance biofilm formation. to date, no studies have focused on the ability of microorganisms isolated from a broad range of environments to coaggregate with each other and it is unclear whether coaggregation promotes the transmission of microorganisms between environmental niches. we aimed to evaluate the coaggregation ability of 29 bacteria and one fungus, isolated from a range of different envir ... | 2015 | 26475462 |
| association of low b cell count and igg levels with infection, and poor vaccine response with all-cause mortality in an immunosuppressed vasculitis population. | patients with systemic vasculitis (sv) have an increased risk of all-cause mortality, often due to infection, compared to the healthy population. we investigated whether humoral response to vaccination and biomarkers of immune dysfunction were associated with infection and death. | 2016 | 26474373 |
| identification of haemophilus haemolyticus in clinical samples and characterization of their mechanisms of antimicrobial resistance. | the objectives of this study were to establish the frequency of haemophilus haemolyticus in clinical samples, to determine the antimicrobial resistance rate and to identify the mechanisms of resistance to β-lactams and quinolones. | 2016 | 26472767 |
| [the national nutrient medium for diagnostic of purulent bacterial meningitis]. | the national growth mediums were developed for isolating and cultivating of main agents of purulent bacterial meningitis--haemophilus agar, chocolate agar, pbm-agar. the growing and selective characteristics of developed growth mediums are examined. the haemophilus agar ensures growth of haemophilus influenzae. the chocolate agar, pbm-agar ensure growth of neisseria meningitidis, streptococcus pneumoniae and haemophilus influenzae. by growing characteristics, the national growth mediums match fo ... | 2015 | 26470444 |
| molecular cloning, expression and purification of truncated hpd fragment of haemophilus influenzae in escherichia coli. | nontypeable haemophilus influenzae (nthi) is a significant pathogen in children, causing otitis media, sinusitis, conjunctivitis, pneumonia, and occasionally invasive infections. protein d (pd) belongs to the minor outer-membrane proteins of h. influenza. moreover, it has been shown that this protein is one of the most potent vaccine candidates against the nthi strain. | 2015 | 26464772 |
| antibiotic resistance of streptococcus pneumoniae in children with acute otitis media treatment failure. | the emergence of antibiotic-resistant bacteria is a major cause of treatment failure in children with acute otitis media (aom). this study aimed to analyze the types of bacterial strains in fluid isolated from the middle ear of children with aom who did not respond to oral antibiotic treatment. we also determined the antibiotic resistance of the most frequently isolated bacterial strain (streptococcus pneumoniae) found in these children. | 2015 | 26454530 |
| analysis of non-typeable haemophilus influenzae in invasive disease reveals lack of the capsule locus. | among invasive haemophilus influenzae, unencapsulated strains have largely surpassed the previously predominant serotype b (hib) because of hib vaccination. isolates without the genomic capsule (cap) locus are designated non-typeable h. influenzae (nthi). they are different from capsule-deficient variants that show deletion of the capsule transport gene bexa within the cap locus. the frequency of capsule-deficient variants in invasive disease is unknown. we analysed 783 unencapsulated invasive i ... | 2016 | 26454058 |
| modelling the impact of vaccination on curtailing haemophilus influenzae serotype 'a'. | haemophilus influenzae serotype a (hia) is a human-restricted bacterial pathogen transmitted via direct contacts with an infectious individual. currently, there is no vaccine available for prevention of hia, and the disease is treated with antibiotics upon diagnosis. with ongoing efforts for the development of an anti-hia protein-polysaccharide conjugated vaccine, we sought to investigate the effect of vaccination on curtailing hia infection. we present the first stochastic model of hia transmis ... | 2015 | 26453974 |
| budesonide inhibits intracellular infection with non-typeable haemophilus influenzae despite its anti-inflammatory effects in respiratory cells and human lung tissue: a role for p38 map kinase. | inhaled corticosteroids (ics) are widely used in the treatment of obstructive lung diseases. recent data suggest a higher pneumonia risk in chronic obstructive pulmonary disease (copd) patients treated with ics. | 2015 | 26452008 |
| invasive nontyphoidal salmonella infections among children in mali, 2002-2014: microbiological and epidemiologic features guide vaccine development. | in 2002, following establishment of a clinical microbiology laboratory in the government hospital that admits children with severe illnesses in bamako, mali, surveillance to identify pathogens causing invasive bacterial infections (septicemia, bacteremia, meningitis, etc) was initiated. | 2015 | 26449949 |
| autotransporter proteins. | this review focuses on the function of the escherichia coli and salmonella autotransporters for which a considerable amount of literature is available. members of the serine protease autotransporters of the enterobacteriaceae (spates) family are proteins from e. coli and shigella spp., which, like the neisseria and haemophilus influenzae iga1 proteases and hap, possess a consensus serine protease motif. the largest subfamily of autotransporters is defined by the aida conserved domain cog3468 and ... | 2005 | 26443517 |
| carriage of haemophilus influenzae in the oropharynx of young children and molecular epidemiology of the isolates after fifteen years of h. influenzae type b vaccination in italy. | haemophilus influenzae is an important pathogen able to cause a wide spectrum of diseases in children. colonization of the upper respiratory tract is a risk factor for developing disease. this study aimed to investigate the oropharyngeal carriage rate of h. influenzae in young children in two italian cities, 15 years after h. influenzae type b (hib) vaccination was introduced. antibiotic resistant traits and genotypes of the colonizing h. influenzae isolates were investigated. | 2015 | 26440924 |
| vitek ms matrix-assisted laser desorption ionization-time of flight mass spectrometry for identifying respiratory bacterial pathogens: a fast and efficient method. | mass spectrometry has become a reference resource for identifying microorganisms in clinical microbiology services. one hundred and fifty one clinical isolates were selected from respiratory specimens routinely identified as streptococcus pneumoniae (43), haemophilus influenzae (64) and moraxella catarrhalis (44). these identifications were compared with other phenotypical methods and mass spectrometry (maldi-tof-ms vitek). result discrepancies were assessed by 16s rrna sequencing. thirty-eight ... | 2015 | 26437754 |
| effectiveness of polyvalent bacterial lysate and autovaccines against upper respiratory tract bacterial colonization by potential pathogens: a randomized study. | polyvalent bacterial lysate (pbl) is an oral immunostimulating vaccine consisting of bacterial standardized lysates obtained by lysis of different strains of bacteria. autovaccines are individually prepared based on the results of smears obtained from the patient. both types of vaccine can be used to treat an ongoing chronic infection. this study sought to determine which method is more effective against nasal colonization by potential respiratory tract pathogens. | 2015 | 26434686 |
| sweet talk: protein glycosylation in bacterial interaction with the host. | pathogenic bacteria encode virulent glycosyltransferases that conjugate various glycans onto substrate proteins via the n- or o-linkage. the hmw system in nontypeable haemophilus influenzae and the pgl system in campylobacter jejuni glycosylate bacterial surface or periplasmic proteins at the eukaryotic-like asn-x-ser/thr motif. the nleb effector from enterobacteria mediates arginine glcnacylation of host death-domain proteins to block inflammation, representing an atypical n-glycosylation. the ... | 2015 | 26433695 |
| qsebc, a two-component bacterial adrenergic receptor and global regulator of virulence in enterobacteriaceae and pasteurellaceae. | the qsebc two-component system (tcs) is associated with quorum sensing and functions as a global regulator of virulence. based on sequence similarity within the sensor domain and conservation of an acidic motif essential for signal recognition, qsebc is primarily distributed in the enterobacteriaceae and pasteurellaceae. in escherichia coli, qsec responds to autoinducer-3 and/or epinephrine/norepinephrine. binding of epinephrine/norepinephrine is inhibited by adrenergic antagonists; hence qsec f ... | 2015 | 26426681 |
| a 15-year retrospective analysis of prognostic factors in childhood bacterial meningitis. | this retrospective chart review aimed to identify factors in childhood bacterial meningitis that predicted disease severity and long-term outcome. | 2016 | 26426265 |
| bacterial exploitation of phosphorylcholine mimicry suppresses inflammation to promote airway infection. | regulation of neutrophil activity is critical for immune evasion among extracellular pathogens, yet the mechanisms by which many bacteria disrupt phagocyte function remain unclear. here, we have shown that the respiratory pathogen streptococcus pneumoniae disables neutrophils by exploiting molecular mimicry to degrade platelet-activating factor (paf), a host-derived inflammatory phospholipid. using mass spectrometry and murine upper airway infection models, we demonstrated that phosphorylcholine ... | 2015 | 26426079 |
| granulomatous-lymphocytic interstitial lung disease and recurrent sinopulmonary infections in a patient with good's syndrome. | good's syndrome is a rare primary immunodeficiency associated with adult thymoma. complications are mainly autoimmune manifestations and recurrent infections with encapsulated bacteria. only one possible case of combined granulomatous-lymphocytic interstitial lung disease (gl-ild) and good's syndrome have been described earlier, but the patient died at the time of diagnosis. this is the first case of gl-ild in good's syndrome with a successful outcome. we present a case of a 43-year-old man with ... | 2015 | 26424818 |
| the clinical presentation of acute bacterial meningitis varies with age, sex and duration of illness. | this swedish study reviewed differences in clinical presentation and laboratory findings of acute bacterial meningitis in children aged one month to 17 years in västerbotten county, sweden. | 2015 | 26421681 |
| significance of tagi and mfd genes in the virulence of non-typeable haemophilus influenzae. | non-typeable haemophilus influenzae (nthi) is an opportunist pathogen well adapted to the human upper respiratory tract and responsible for many respiratory diseases. in the human airway, nthi is exposed to pollutants, such as alkylating agents, that damage its dna. in this study, we examined the significance of genes involved in the repair of dna alkylation damage in nthi virulence. two knockout mutants, tagi and mfd, encoding n³-methyladenine-dna glycosylase i and the key protein involved in t ... | 2014 | 26419455 |
| genome expression profiling-based identification and administration efficacy of host-directed antimicrobial drugs against respiratory infection by nontypeable haemophilus influenzae. | therapies that are safe, effective, and not vulnerable to developing resistance are highly desirable to counteract bacterial infections. host-directed therapeutics is an antimicrobial approach alternative to conventional antibiotics based on perturbing host pathways subverted by pathogens during their life cycle by using host-directed drugs. in this study, we identified and evaluated the efficacy of a panel of host-directed drugs against respiratory infection by nontypeable haemophilus influenza ... | 2015 | 26416856 |
| [quality of immunization data in children aged 0 to 11 months in côte d’ivoire]. | in order to guide the decisions of programme managers, an immunization data quality self-assessment was performed in côte d’ivoire in 2012. this study was designed to assess the accuracy of immunization data and the quality of the immunization tracking system with this tool. | 2015 | 26414040 |
| the costs and effectiveness of large phase iii pre-licensure vaccine clinical trials. | prior to the 1980s, most vaccines were licensed based upon safety and effectiveness studies in several hundred individuals. beginning with the evaluation of haemophilus influenzae type b conjugate vaccines, much larger pre-licensure trials became common. the pre-licensure trial for haemophilus influenzae oligosaccharide conjugate vaccine had more than 60,000 children and that of the seven-valent pneumococcal conjugate vaccine included almost 38,000 children. although trial sizes for both of thes ... | 2015 | 26414015 |
| analysis of the distribution and evolution of the atp-dependent dna ligases of bacteria delineates a distinct phylogenetic group 'lig e'. | prior to the discovery of a minimal atp-dependent dna ligase in haemophilus influenzae, bacteria were thought to only possess a nad-dependent ligase, which was involved in sealing of okazaki fragments. we now know that a diverse range of bacterial species possess up to six of these accessory bacterial atp-dependent dna ligases (b-adls), which vary in size and enzymatic domain associations. here we compare the domain structure of different types of b-adls and investigate their distribution among ... | 2016 | 26412580 |
| toddler with fever and grunting. | 2015 | 26407254 | |
| invasive disease caused by nontypeable haemophilus influenzae. | 2015 | 26407156 | |
| draft genome sequences of six nontypeable haemophilus influenzae strains that establish bacteremia in the infant rat model of invasive disease. | haemophilus influenzae is an important cause of invasive disease. the infant rat is the accepted model of invasive h. influenzae disease. here, we report the genome sequences of six nontypeable h. influenzae strains that establish bacteremia in the infant rat. | 2015 | 26404588 |
| respiratory bacterial culture from two sequential bronchoalveolar lavages of the same lobe in children with chronic cough. | identification of bacteria causing lower-airway infections is important to determine appropriate antimicrobial therapy. flexible bronchoscopy with bronchoalveolar lavage (bal) is used to obtain lower-airway specimens in young children. the first lavage (lavage-1) is typically used for bacterial culture. however, no studies in children have compared the detection of cultivable bacteria from sequential lavages of the same lobe. bal fluid was collected from two sequential lavages of the same lobe i ... | 2015 | 26399701 |
| safety and immunogenicity of a quadrivalent meningococcal conjugate vaccine and commonly administered vaccines after coadministration. | given the broad age range across which the quadrivalent meningococcal conjugate vaccine menacwy-crm is used, coadministration with routine vaccines should be evaluated across age groups for possible immunologic interference and impact on vaccine reactogenicity and safety. | 2016 | 26398743 |
| phylogenetic relationship of non-typeable haemophilus influenzae isolated in malaysia. | the epidemiology of non-typeable haemophilus influenzae (nthi) remains poorly understood. we therefore sought to determine the genetic relationship of 25 nthi isolated from various states in malaysia using multilocus sequence typing (mlst). the majority of isolates were obtained from sputum. there were 24 novel sequence types (sts). eight isolates were single-locus variants, the remainder being singletons. clustering was not based on clinical site of isolation or geographical origin. despite the ... | 2015 | 26394107 |
| frequency of pathogenic paediatric bacterial meningitis in mozambique: the critical role of multiplex real-time polymerase chain reaction to estimate the burden of disease. | in sub-saharan africa, including mozambique, acute bacterial meningitis (abm) represents a main cause of childhood mortality. the burden of abm is seriously underestimated because of the poor performance of culture sampling, the primary method of abm surveillance in the region. low quality cerebrospinal fluid (csf) samples and frequent consumption of antibiotics prior to sample collection lead to a high rate of false-negative results. to our knowledge, this study is the first to determine the fr ... | 2015 | 26393933 |
| antisera against certain conserved surface-exposed peptides of nontypeable haemophilus influenzae are protective. | nontypeable haemophilus influenzae (nthi) cause significant disease, including otitis media in children, exacerbations of chronic obstructive pulmonary disease, and invasive disease in susceptible populations. no vaccine is currently available to prevent nthi disease. the interactions of nthi and the human host are primarily mediated by lipooligosaccharide and a complex array of surface-exposed proteins (seps) that act as receptors, sensors and secretion systems. we hypothesized that certain sep ... | 2015 | 26390432 |
| tem-1-encoding small plasmids impose dissimilar fitness costs on haemophilus influenzae and haemophilus parainfluenzae. | only two beta-lactamases, tem-1 and rob-1, have been observed in haemophilus influenzae, while four different tem but no rob enzymes have been found in haemophilus parainfluenzae. in order to investigate the mechanisms behind the dissemination of small beta-lactamase-encoding plasmids in h. influenzae and h. parainfluenzae, we assessed the fitness cost of three tem-1- (ppn223, pa1209, pa1606), one tem-15- (psf3) and one rob-1-bearing (pb1000) plasmid when expressed in either bacterial species. a ... | 2015 | 26385154 |
| progressive ventilation inhomogeneity in infants with cystic fibrosis after pulmonary infection. | measures of ventilation distribution are promising for monitoring early lung disease in cystic fibrosis (cf). this study describes the cross-sectional and longitudinal impacts of pulmonary inflammation and infection on ventilation homogeneity in infants with cf.infants diagnosed with cf underwent multiple breath washout (mbw) testing and bronchoalveolar lavage at three time points during the first 2 years of life.measures were obtained for 108 infants on 156 occasions. infants with a significant ... | 2015 | 26381521 |
| evidence for infection and inflammation in infant deaths in a country with historically low incidences of sudden infant death syndrome. | total infant mortality in hungary has been higher than other european countries; however, the reported incidence of sudden infant death syndrome (sids) has been lower. the low incidence of sids in hungary has been supported by evidence obtained from the high rate of scene of death investigation and medico-legal autopsy mandatory since the 1950s. in this study, we compared the incidence of explained and unexplained infant deaths in hungary for three periods: 1979-1989 when the incidence of sids w ... | 2015 | 26379661 |
| complete deletion of the fucose operon in haemophilus influenzae is associated with a cluster in multilocus sequence analysis-based phylogenetic group ii related to haemophilus haemolyticus: implications for identification and typing. | nonhemolytic variants of haemophilus haemolyticus are difficult to differentiate from haemophilus influenzae despite a wide difference in pathogenic potential. a previous investigation characterized a challenging set of 60 clinical strains using multiple pcrs for marker genes and described strains that could not be unequivocally identified as either species. we have analyzed the same set of strains by multilocus sequence analysis (mlsa) and near-full-length 16s rrna gene sequencing. mlsa unambig ... | 2015 | 26378279 |
| septic arthritis in infants younger than 3 months: a retrospective review. | septic arthritis in infants is rare and can be difficult to diagnose. this study reviewed a series of patients younger than 3 months to identify factors that may assist in early diagnosis and treatment. a query of records at a large midwestern pediatric hospital (1994-2010) was performed to identify all patients younger than 3 months at the time of diagnosis. analysis included birth history, joint involvement, physical examination findings, laboratory results, imaging results, method of treatmen ... | 2015 | 26375536 |
| antibody responses after primary immunization in infants born to women receiving a pertussis-containing vaccine during pregnancy: single arm observational study with a historical comparator. | in england, antenatal pertussis immunization using a tetanus/low-dose diphtheria/5-component acellular-pertussis/inactivated-polio (tdap5/ipv) vaccine was introduced in october 2012. we assessed infant responses to antigens in the maternal vaccine and to those conjugated to tetanus (tt) or the diphtheria toxin variant, crm. | 2015 | 26374816 |
| five-year antibody persistence and safety after a single dose of combined haemophilus influenzae type b neisseria meningitidis serogroup c-tetanus toxoid conjugate vaccine in haemophilus influenzae type b-primed toddlers. | antibody persistence is evaluated in healthy australian children 4 and 5 years postvaccination with a single dose of combined haemophilus influenzae type b-neisseria meningitidis serogroup c tetanus toxoid conjugate vaccine (hib-menc-tt) compared with separately administered hib-tt and menc-crm197 vaccines (hib + mcc). | 2015 | 26372451 |
| update on routine childhood and adolescent immunizations. | recommendations for routine vaccinations in children and adolescents have changed multiple times in recent years, based on findings in clinical trials, licensure of new vaccines, and evidence of waning immunity. despite the overwhelming success of vaccinations, vaccine delay and refusal are leading to pockets of vaccine-preventable diseases. schedules for diphtheria and tetanus toxoids, and acellular pertussis (dtap); hepatitis a and b; haemophilus influenzae type b (hib); inactivated poliovirus ... | 2015 | 26371731 |
| determinants of bacteriological outcomes in exacerbations of chronic obstructive pulmonary disease. | changes in sputum microbiology following antibiotic treatment of acute exacerbations of chronic obstructive pulmonary disease (aecopd), including patterns of bacteriological relapse and superinfection are not well understood. sputum microbiology at exacerbation is not routinely performed, but pathogen presence and species are determinants of outcomes. therefore, we determined whether baseline clinical factors could predict the presence of bacterial pathogens at exacerbation. bacterial eradicatio ... | 2015 | 26370552 |
| improvement of antibiotic therapy and icu survival in severe non-pneumococcal community-acquired pneumonia: a matched case-control study. | we aimed to compare intensive care unit mortality due to non-pneumococcal severe community-acquired pneumonia between the periods 2000-2002 and 2008-2014, and the impact of the improvement in antibiotic strategies on outcomes. | 2015 | 26369551 |
| acquired macrolide resistance genes in haemophilus influenzae?-authors' response. | 2015 | 26369355 | |
| epidemiology of invasive pneumococcal disease in saudi arabian children younger than 5years of age. | this study evaluated the incidence, serotype distribution, and antimicrobial susceptibility of invasive pneumococcal disease (ipd) in saudi arabian children. this multicenter, prospective, clinical surveillance study included children under 5years of age, residents of one of the seven study health areas, who were brought to a study hospital with suspicion of ipd. bacterial isolates from sterile site samples, collected less than 24h after hospital visit/admission, were identified, serotyped, and ... | 2016 | 26368823 |
| population density profiles of nasopharyngeal carriage of 5 bacterial species in pre-school children measured using quantitative pcr offer potential insights into the dynamics of transmission. | bacterial vaccines can reduce carriage rates. colonization is usually a binary endpoint. real time quantitative pcr (qpcr) can quantify bacterial dna in mucosal samples over a wide range. using culture and single-gene species-specific qpcrs for streptococcus pneumoniae (lyta), streptococcus pyogenes (ntpc), moraxella catarrhalis (ompj), haemophilus influenzae (hdp) and staphylococcus aureus (nuc) and standard curves against log-phase reference strain broth cultures we described frequency and pea ... | 2015 | 26367344 |
| vaccination uptake by vaccine-hesitant parents attending a specialist immunization clinic in australia. | vaccine hesitancy (vh) is an issue of global concern. the quality of communication between healthcare providers and parents can influence parental immunization acceptance. we aimed to describe immunization uptake following specialist immunization clinic (sic) consultation for australian children of vh parents as a cohort, and according to pre-clinic parental position on immunization. at a single tertiary pediatric sic (rch, melbourne) a retrospective descriptive study classified vh families acco ... | 2015 | 26366978 |
| impact of bacteria in nasal aspirates on disease severity of bronchiolitis. | the effect of potentially pathogenic bacteria (ppb) on disease severity in patients with bronchiolitis is understudied. | 2016 | 26365560 |
| nasopharyngeal carriage and macrolide resistance in indigenous children with bronchiectasis randomized to long-term azithromycin or placebo. | although long-term azithromycin decreases exacerbation frequency in bronchiectasis, increased macrolide resistance is concerning. we investigated macrolide resistance determinants in a secondary analysis of a multicenter randomized controlled trial. indigenous australian children living in remote regions and urban new zealand māori and pacific islander children with bronchiectasis were randomized to weekly azithromycin (30 mg/kg) or placebo for up to 24 months and followed post-intervention for ... | 2015 | 26363637 |