Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| selection of a suitable disc bioassay for the screening of anti-tumor molecules. | the crown gall induced in potato discs by agrobacterium tumefaciens is becoming largely utilised in screening anti-tumor agents. the present work is showing that beet discs are more adequate for the anti-tumor screening test. in fact, maximal tumor induction was observed on beet discs (87.5%), followed by carrot discs (75%) and potato discs (68.5%). beet discs present the most sensibility to crown gall disease with a fast expression of symptoms and more visible galls without any staining need. t ... | 2013 | 24711759 |
| genome sequence of ensifer adhaerens ov14 provides insights into its ability as a novel vector for the genetic transformation of plant genomes. | recently it has been shown that ensifer adhaerens can be used as a plant transformation technology, transferring genes into several plant genomes when equipped with a ti plasmid. for this study, we have sequenced the genome of ensifer adhaerens ov14 (ov14) and compared it with those of agrobacterium tumefaciens c58 (c58) and sinorhizobium meliloti 1021 (1021); the latter of which has also demonstrated a capacity to genetically transform crop genomes, albeit at significantly reduced frequencies. | 2014 | 24708309 |
| a simple and reliable multi-gene transformation method for switchgrass. | a simple and reliable agrobacterium -mediated transformation method was developed for switchgrass. using this method, many transgenic plants carrying multiple genes-of-interest could be produced without untransformed escape. switchgrass (panicum virgatum l.) is a promising biomass crop for bioenergy. to obtain transgenic switchgrass plants carrying a multi-gene trait in a simple manner, an agrobacterium-mediated transformation method was established by constructing a gateway-based binary vector, ... | 2014 | 24700247 |
| tawrky68 responses to biotic stresses are revealed by the orthologous genes from major cereals. | wrky transcription factors have been extensively characterized in the past 20 years, but in wheat, studies on wrky genes and their function are lagging behind many other species. to explore the function of wheat wrky genes, we identified a tawrky68 gene from a common wheat cultivar. it encodes a protein comprising 313 amino acids which harbors 19 conserved motifs or active sites. gene expression patterns were determined by analyzing microarray data of tawrky68 in wheat and of orthologous genes f ... | 2014 | 24688294 |
| pepper suppressor of the g2 allele of skp1 interacts with the receptor-like cytoplasmic kinase1 and type iii effector avrbst and promotes the hypersensitive cell death response in a phosphorylation-dependent manner. | xanthomonas campestris pv vesicatoria type iii effector protein, avrbst, triggers hypersensitive cell death in pepper (capsicum annuum). here, we have identified the pepper sgt1 (for suppressor of the g2 allele of skp1) as a host interactor of avrbst and also the pepper pik1 (for receptor-like cytoplasmic kinase1). pik1 specifically phosphorylates sgt1 and avrbst in vitro. avrbst specifically binds to the chord-containing protein and sgt1 domain of sgt1, resulting in the inhibition of pik1-media ... | 2014 | 24686111 |
| identification of the bona fide dhdps from a common plant pathogen. | agrobacterium tumefaciens is a gram-negative soil-borne bacterium that causes crown gall disease in many economically important crops. the absence of a suitable chemical treatment means there is a need to discover new anti-crown gall agents and also characterize bona fide drug targets. one such target is dihydrodipicolinate synthase (dhdps), a homo-tetrameric enzyme that catalyzes the committed step in the metabolic pathway yielding meso-diaminopimelate and lysine. interestingly, there are 10 pu ... | 2014 | 24677246 |
| genome sequence of the octopine-type agrobacterium tumefaciens strain ach5. | we have sequenced the complete genome of the plant pathogen agrobacterium tumefaciens strain lba4213, a derivative of the wild-type strain a. tumefaciens ach5 and the ancestor of a. tumefaciens strain lba4404 used in genetic engineering. the genome consists of a circular chromosome and a linear chromosome, as well as a megaplasmid and a tumor-inducing plasmid. | 2014 | 24675863 |
| involvement of the acr3 and dcta anti-porters in arsenite oxidation in agrobacterium tumefaciens 5a. | microbial arsenite (asiii) oxidation forms a critical piece of the arsenic cycle in nature, though our understanding of how and why microorganisms oxidize asiii remains rudimentary. our model organism agrobacterium tumefaciens 5a contains two distinct ars operons (ars1 and ars2) that are similar in their coding region content. the ars1 operon is located nearby the aio operon that is essential for asiii oxidation. the asiii/h(+) anti-porters encoded by acr3-1 and acr3-2 are required for maximal a ... | 2015 | 24674103 |
| fate of arsenate following arsenite oxidation in agrobacterium tumefaciens gw4. | the fate of arsenate (as(v) ) generated by microbial arsenite (as(iii) ) oxidation is poorly understood. agrobacterium tumefaciens wild-type strain (gw4) was studied to determine how the cell copes with as(v) generated in batch culture. gw4 grown heterotrophically with mannitol used as(iii) as a supplemental energy supply as reflected by enhanced growth and increased cellular levels of nadh and atp. under low phosphate (pi) conditions and presence of as(iii) oxidation, up to ∼ 50% of the resulti ... | 2015 | 24673976 |
| antimicrobial activities of novel mannosyl lipids isolated from the biocontrol fungus simplicillium lamellicola bcp against phytopathogenic bacteria. | the antagonistic fungus simplicillium lamellicola bcp has been developed as a microbial biopesticide that effectively controls the development of various plant diseases caused by both pathogenic bacteria and pathogenic fungi. antibacterial bioassay-directed fractionation was used to isolate mannosyl lipids from s. lamellicola bcp, and the structures of these compounds were elucidated using spectral analysis and chemical degradation. three novel mannosyl lipids were characterized and identified a ... | 2014 | 24660753 |
| analysis of hydroxycinnamic acid degradation in agrobacterium fabrum reveals a coenzyme a-dependent, beta-oxidative deacetylation pathway. | the soil- and rhizosphere-inhabiting bacterium agrobacterium fabrum (genomospecies g8 of the agrobacterium tumefaciens species complex) is known to have species-specific genes involved in ferulic acid degradation. here, we characterized, by genetic and analytical means, intermediates of degradation as feruloyl coenzyme a (feruloyl-coa), 4-hydroxy-3-methoxyphenyl-β-hydroxypropionyl-coa, 4-hydroxy-3-methoxyphenyl-β-ketopropionyl-coa, vanillic acid, and protocatechuic acid. the genes atu1416, atu14 ... | 2014 | 24657856 |
| expression of a fungal ferulic acid esterase in alfalfa modifies cell wall digestibility. | alfalfa (medicago sativa) is an important forage crop in north america owing to its high biomass production, perennial nature and ability to fix nitrogen. feruloyl esterase (ec 3.1.1.73) hydrolyzes ester linkages in plant cell walls and has the potential to further improve alfalfa as biomass for biofuel production. | 2014 | 24650274 |
| cloning, transformation and expression of proinsulin gene in tomato (lycopersicum esculentum mill.). | plants are among promising and suitable platform systems for production of recombinant biopharmaceutical proteins due to several features such as safety, no need for fermentation, inexpensive investment, and fast and easy scale-up. human insulin is one of the most widely used medicines in the world. up to now different expression systems including escherichia coli, yeast and cho have been exploited for producing recombinant human insulin and a variety of different recombinant insulin are extensi ... | 2014 | 24644433 |
| virus-induced gene silencing and agrobacterium tumefaciens-mediated transient expression in nicotiana tabacum. | virus-induced gene silencing (vigs) is a rapid method for transient silencing of plant genes. in this chapter, we describe the methodology for tobacco rattle virus (trv)-based vigs in nicotiana tabacum. in combination with subsequent co-expression of the tomato immune receptor ve1 and the corresponding verticillium effector ave1 through agrobacterium tumefaciens-mediated transient transformation (agroinfiltration), we established a rapid system for assessing the requirement of candidate plant ge ... | 2014 | 24643561 |
| structural basis for high substrate-binding affinity and enantioselectivity of 3-quinuclidinone reductase atqr. | (r)-3-quinuclidinol, a useful compound for the synthesis of various pharmaceuticals, can be enantioselectively produced from 3-quinuclidinone by 3-quinuclidinone reductase. recently, a novel nadh-dependent 3-quinuclidionone reductase (atqr) was isolated from agrobacterium tumefaciens, and showed much higher substrate-binding affinity (>100 fold) than the reported 3-quinuclidionone reductase (rrqr) from rhodotorula rubra. here, we report the crystal structure of atqr at 1.72 å. three nadh-bound p ... | 2014 | 24642255 |
| agrobacterium tumefaciens-mediated transformation of lasiodiplodia theobromae, the causal agent of gummosis in cashew nut plants. | lasiodiplodia theobromae is a major pathogen of many different crop cultures, including cashew nut plants. this paper describes an efficient agrobacterium tumefaciens-mediated transformation (atmt) system for the successful delivery of t-dna, transferring the genes of green fluorescent protein (gfp) and hygromycin b phosphotransferase (hph) to l. theobromae. when the fungal pycnidiospores were co-cultured with a. tumefaciens harboring the binary vector with hph-gfp gene, hygromycin-resistant fun ... | 2014 | 24634294 |
| t-dna of a crown gall teratoma is covalently joined to host plant dna. | agrobacterium tumefaciens strains containing tumour-inducing (ti) plasmids incite cancerous growths called crown galls when inoculated into wounded dicotyledonous plants. tumour tissue can be cultured axenically in vitro, and exhibits a transformed phenotype in the absence of the inciting bacterium. transformed cells grow autonomously, are auxin and cytokinin autotrophic in vitro and synthesize opines, novel amino acid derivatives dictated by ti plasmid genetic information. a small segment of th ... | 1980 | 24627935 |
| fha interaction with phosphothreonine of tssl activates type vi secretion in agrobacterium tumefaciens. | the type vi secretion system (t6ss) is a widespread protein secretion system found in many gram-negative bacteria. t6sss are highly regulated by various regulatory systems at multiple levels, including post-translational regulation via threonine (thr) phosphorylation. the ser/thr protein kinase ppka is responsible for this thr phosphorylation regulation, and the forkhead-associated (fha) domain-containing fha-family protein is the sole t6ss phosphorylation substrate identified to date. here we d ... | 2014 | 24626341 |
| dimerization of vird2 binding protein is essential for agrobacterium induced tumor formation in plants. | the type iv secretion system (t4ss) is the only bacterial secretion system known to translocate both dna and protein substrates. the virb/d4 system from agrobacterium tumefaciens is a typical t4ss. it facilitates the bacteria to translocate the vird2-t-dna complex to the host cell cytoplasm. in addition to protein-dna complexes, the virb/d4 system is also involved in the translocation of several effector proteins, including vire2, vire3 and virf into the host cell cytoplasm. these effector prote ... | 2014 | 24626239 |
| cloning, transformation and expression of human interferon α2b gene in tobacco plant (nicotiana tabacum cv. xanthi). | molecular farming is the production of important recombinant proteins in transgenic organisms on an agricultural scale. interferons are proteins with antiviral and antitumor activities and can be used for viral infections and cancers treatments. | 2012 | 24624166 |
| [genetic transformation of the fungus gliocladium sp. mediated by agrobacterium tumefaciens]. | to construct a transformation system in gliocladium sp. 6.102, a cordyceps-colonizing fungus producing a variety of epipolythiodioxopiperazine (etp) compounds with drug potentials. | 2013 | 24617266 |
| attachment of agrobacterium tumefaciens to leaf tissue in response to infiltration conditions. | transient expression of recombinant proteins in plant tissues following agrobacterium-mediated gene transfer is a promising technique for rapid protein production. however, transformation rates and transient expression levels can be sub-optimal depending on process conditions. attachment of agrobacterium tumefaciens to plant cells is an early, critical step in the gene transfer pathway. bacterial attachment levels and patterns may influence transformation and, by extension, transient expression. ... | 2014 | 24616410 |
| an efficient agrobacterium-mediated transformation method for the edible mushroom hypsizygus marmoreus. | hypsizygus marmoreus is one of the major edible mushrooms in east asia. as no efficient transformation method, the molecular and genetics studies were hindered. the glyceraldehyde-3-phosphate dehydrogenase (gpd) gene of h. marmoreus was isolated and its promoter was used to drive the hygromycin b phosphotransferase (hph) and enhanced green fluorescent protein (egfp) in h. marmoreus. agrobacterium tumefaciens-mediated transformation (atmt) was successfully applied in h. marmoreus. the transformat ... | 2014 | 24612605 |
| in vivo self-assembly of stable green fluorescent protein fusion particles and their uses in enzyme immobilization. | bacterial inclusion bodies are aggregations of mostly inactive and misfolded proteins. however, previously the in vivo self-assembly of green fluorescent protein (gfp) fusions into fluorescent particles which displayed specific binding sites suitable for applications in bioseparation and diagnostics was demonstrated. here, the suitability of gfp particles for enzyme immobilization was assessed. the enzymes tested were a thermostable α-amylase from bacillus licheniformis, n-acetyl-d-neuraminic ac ... | 2014 | 24610847 |
| overexpression of s-adenosyl-l-methionine synthetase increased tomato tolerance to alkali stress through polyamine metabolism. | s-adenosyl-l-methionine (sam) synthetase is the key enzyme involved in the biosynthesis of sam, which serves as a common precursor for polyamines (pas) and ethylene. a sam synthetase cdna (slsams1) was introduced into the tomato genome using the agrobacterium tumefaciens transformation method. transgenic plants overexpressing slsams1 exhibited a significant increase in tolerance to alkali stress and maintained nutrient balance, higher photosynthetic capacity and lower oxidative stress compared w ... | 2014 | 24605920 |
| transgenic pearl millet male fertility restorer line (icmp451) and hybrid (icmh451) expressing brassica juncea nonexpressor of pathogenesis related genes 1 (bjnpr1) exhibit resistance to downy mildew disease. | brassica juncea nonexpressor of pathogenesis-related genes 1 (bjnpr1) has been introduced into pearl millet male fertility restorer line icmp451 by agrobacterium tumefaciens-mediated genetic transformation. transgenic pearl millet plants were regenerated from the phosphinothricin-resistant calli obtained after co-cultivation with a. tumefaciens strain lba4404 harbouring ti plasmid psb111-bar-bjnpr1. molecular analyses confirmed the stable integration and expression of bjnpr1 in transgenic pearl ... | 2014 | 24603762 |
| transgenic tomato line expressing modified bacillus thuringiensis cry1ab gene showing complete resistance to two lepidopteran pests. | the modified truncated bt-cry1ab gene of bacillus thuringiensis has been used for the development and selection of over expressing transgenic events in a commercially important variety of tomato (solanum lycopersicum l.) by agrobacterium-mediated leaf-disc transformation procedure. the integration and inheritance of cry1ab gene in t0 transgenic plants and their progenies were determined by pcr, rt-pcr and southern blot hybridization analysis. the toxin expression was monitored by double antibody ... | 2014 | 24600542 |
| roles of agrobacterium tumefaciens membrane-bound ferritin (mbfa) in iron transport and resistance to iron under acidic conditions. | agrobacterium tumefaciens membrane-bound ferritin (mbfa) is a member of the erythrin (er)-vacuolar iron transport family. the mbfa protein has an er or ferritin-like domain at its n terminus and has been predicted to have five transmembrane segments in its c-terminal region. analysis of protein localization using phoa and lacz reporter proteins supported the view that the n-terminal di-iron site is located in the cytoplasm whilst the c-terminal end faces the periplasm. an a. tumefaciens mbfa mut ... | 2014 | 24600024 |
| a new high-frequency agrobacterium-mediated transformation technique for sesamum indicum l. using de-embryonated cotyledon as explant. | in spite of the economic importance of sesame (sesamum indicum l.) and the recent availability of its genome sequence, a high-frequency transformation protocol is still not available. the only two existing agrobacterium-mediated transformation protocols that are available have poor transformation efficiencies of less than 2%. in the present study, we report a high-frequency, simple, and reproducible transformation protocol for sesame. transformation was done using de-embryonated cotyledons via s ... | 2014 | 24590594 |
| a procedure for the transient expression of genes by agroinfiltration above the permissive threshold to study temperature-sensitive processes in plant-pathogen interactions. | localized expression of genes in plants from t-dnas delivered into plant cells by agrobacterium tumefaciens is an important tool in plant research. the technique, known as agroinfiltration, provides fast, efficient ways to transiently express or silence a desired gene without resorting to the time-consuming, challenging stable transformation of the host, the use of less efficient means of delivery, such as bombardment, or the use of viral vectors, which multiply and spread within the host causin ... | 2014 | 24581046 |
| enhancers of agrobacterium-mediated transformation of tibouchina semidecandra selected on the basis of gfp expression. | genetic engineering is a powerful tool for the improvement of plant traits. despite reported successes in the plant kingdom, this technology has barely scratched the surface of the melastomataceae family. limited studies have led to some optimisation of parameters known to affect the transformation efficiency of these plants. the major finding of this study was to optimise the presence of selected enhancers [e.g., monosaccharides (d-glucose, d-galactose and d-fructose), tyrosine, aluminium chlor ... | 2010 | 24575204 |
| rnai mediated curcin precursor gene silencing in jatropha (jatropha curcas l.). | curcin, a type i ribosomal inhibiting protein-rip, encoded by curcin precursor gene, is a phytotoxin present in jatropha (jatropha curcas l.). here, we report designing of rnai construct for the curcin precursor gene and further its genetic transformation of jatropha to reduce its transcript expression. curcin precursor gene was first cloned from jatropha strain darl-2 and part of the gene sequence was cloned in sense and antisense orientation separated by an intron sequence in plant expression ... | 2014 | 24574003 |
| [isolation and characterization of petroleum catabolic broad-host-range plasmids from shen-fu wastewater irrigation zone]. | based on triparental mating, we isolated a total of eight broad host range (bhr) petroleum hydrocarbon catabolic plasmids from the soils, sediments, and wastewater samples in the shen-fu irrigation zone. the antibiotic resistance of the plasmids was tested, and then, the plasmids were transferred to escherichia coli ec100. the plasmids carrying no antibiotic resistance were tagged by minitn5 transposon consisting of antibiotic resistant genes. the pcr-based incompatibility test revealed that the ... | 2013 | 24564162 |
| improved dominant selection markers and co-culturing conditions for efficient agrobacterium tumefaciens-mediated transformation of ustilago scitaminea. | ustilago scitaminea is the causal agent of sugar-cane smut disease. there is, however, no genetic transformation method for it. here we report the development of an efficient mutagenesis method based on agrobacterium tumefaciens-mediated transformation. to improve transformation efficiency, a range of conditions, including the codon-usage preference of the selection marker gene, promoters and the culture conditions for transformation were optimized. a strong promoter to drive marker gene express ... | 2014 | 24563317 |
| a bifunctional glycosyltransferase from agrobacterium tumefaciens synthesizes monoglucosyl and glucuronosyl diacylglycerol under phosphate deprivation. | glycolipids are mainly found in phototrophic organisms (like plants and cyanobacteria), in gram-positive bacteria, and a few other bacterial phyla. besides the function as bulk membrane lipids, they often play a role under phosphate deprivation as surrogates for phospholipids. the gram-negative agrobacterium tumefaciens accumulates four different glycolipids under phosphate deficiency, including digalactosyl diacylglycerol and glucosylgalactosyl diacylglycerol synthesized by a processive glycosy ... | 2014 | 24558041 |
| agrobacterium tumefaciens-mediated transformation of the causative agent of valsa canker of apple tree valsa mali var. mali. | valsa mali var. mali (vmm), which is the causative agent of valsa canker of apple tree, causes heavy damage to apple production in eastern asia. in this article, we report agrobacterium tumefaciens-mediated transformation (atmt) of vmm and expression of gfp (green fluorescent protein) in this fungus. the transformation system was optimized to a transformation efficiency of approximately 150 transformants/10(6) conidia, and a library containing over 4,000 transformants was generated. the tested t ... | 2014 | 24554343 |
| the influence of heat stress on auxin distribution in transgenic b. napus microspores and microspore-derived embryos. | plant embryogenesis is regulated by differential distribution of the plant hormone auxin. however, the cells establishing these gradients during microspore embryogenesis remain to be identified. for the first time, we describe, using the dr5 or dr5rev reporter gene systems, the gfp- and gus-based auxin biosensors to monitor auxin during brassica napus androgenesis at cellular resolution in the initial stages. our study provides evidence that the distribution of auxin changes during embryo develo ... | 2014 | 24553810 |
| functions and regulation of quorum-sensing in agrobacterium tumefaciens. | in agrobacterium tumefaciens, horizontal transfer and vegetative replication of oncogenic ti plasmids involve a cell-to-cell communication process called quorum-sensing (qs). the determinants of the qs-system belong to the luxr/luxi class. the luxi-like protein trai synthesizes n-acyl-homoserine lactone molecules which act as diffusible qs-signals. beyond a threshold concentration, these molecules bind and activate the luxr-like transcriptional regulator trar, thereby initiating the qs-regulator ... | 2014 | 24550924 |
| development of an agrobacterium-mediated stable transformation method for the sensitive plant mimosa pudica. | the sensitive plant mimosa pudica has long attracted the interest of researchers due to its spectacular leaf movements in response to touch or other external stimuli. although various aspects of this seismonastic movement have been elucidated by histological, physiological, biochemical, and behavioral approaches, the lack of reverse genetic tools has hampered the investigation of molecular mechanisms involved in these processes. to overcome this obstacle, we developed an efficient genetic transf ... | 2014 | 24533121 |
| [submerse bacterial plant tumor on roots]. | spontaneous infections seem to indicate that not only the crown of herbeous plants is disposed to generate tumors. under substrate conditions such as those normally found at the crown, infections caused by agrobacterium tumefaciens will yield tumors in the entire area of the roots.roots of helianthus annuus and solanum lycopersicum grown in nutritive medium are disposed to tumor when given appropriate conditions of culture and inoculation. tissue culture, the most widely applied method for the a ... | 1968 | 24522818 |
| the brucella suis ibpa heat-shock chaperone is not required for virulence or for expression of the virb type iv secretion system virb8 protein. | brucella suis, facultative intracellular bacterial pathogen of mammals, and agrobacterium tumefaciens, a plant pathogen, both use a virb type iv secretion system (t4ss) to translocate effector molecules into host cells. hspl, an α-crystalline-type small heat-shock protein, acts as a chaperone for the agrobacterium virb8 protein, an essential component of the virb system. an agrobacterium mutant lacking hspl is attenuated due to a misfunctional t4ss. we have investigated whether ibpa (bra0051), t ... | 2014 | 24517122 |
| transformation of morinda citrifolia via simple mature seed imbibition method. | morinda citrifolia, is a valuable medicinal plant with a wide range of therapeutic properties and extensive transformation study on this plant has yet been known. present study was conducted to establish a simple and reliable transformation protocol for m. citrifolia utilising agrobacterium tumefaciens via direct seed exposure. in this study, the seeds were processed by tips clipping and dried and subsequently incubated in inoculation medium. four different parameters during the incubation such ... | 2013 | 24517006 |
| improvement of a gene targeting system for genetic manipulation in penicillium digitatum. | penicillium digitatum is the most important pathogen of postharvest citrus. gene targeting can be done in p. digitatum using homologous recombination via agrobacterium tumefaciens mediated transformation (atmt), but the frequencies are often very low. in the present study, we replaced the ku80 homolog (a gene of the non-homologous end-joining (nhej) pathway) with the hygromycin resistance cassette (hph) by atmt. no significant change in vegetative growth, conidiation, or pathogenicity was observ ... | 2014 | 24510704 |
| non-additive costs and interactions alter the competitive dynamics of co-occurring ecologically distinct plasmids. | plasmids play an important role in shaping bacterial evolution and adaptation to heterogeneous environments. as modular genetic elements that are often conjugative, the selective pressures that act on plasmid-borne genes are distinct from those that act on the chromosome. many bacteria are co-infected by multiple plasmids that impart niche-specific phenotypes. thus, in addition to host-plasmid dynamics, interactions between co-infecting plasmids are likely to be important drivers of plasmid popu ... | 2014 | 24500159 |
| [the chlorophyll content of tumors from pale and green leaf tissues of kalanchoe fedtschenkoi]. | pale and green leaves of kalanchoe fedtschenkoi var. variegata were inoculated with agrobacterium tumefaciens. six weeks later the chlorophyll contents of the leaves and their tumors were investigated. the tumor tissues possess very similar concentrations of chlorophylls and a low number of chloroplasts per cell. thereby they differ from each of the initial tissues. the qualitative composition of pigments (chlorophylls and carotenoids) of the 4 tissues is the same. | 1970 | 24500130 |
| isolation and molecular characterization of biofouling bacteria and profiling of quorum sensing signal molecules from membrane bioreactor activated sludge. | the formation of biofilm in a membrane bioreactor depends on the production of various signaling molecules like n-acyl homoserine lactones (ahls). in the present study, a total of 200 bacterial strains were isolated from membrane bioreactor activated sludge and screened for ahls production using two biosensor systems, chromobacterium violaceum cv026 and agrobacterium tumefaciens a136. a correlation between ahls production and biofilm formation has been made among screened ahls producing strains. ... | 2014 | 24499972 |
| indica rice cultivar irga 424, transformed with cry genes of b. thuringiensis, provided high resistance against spodoptera frugiperda (lepidoptera: noctuidae). | plant expression of the entomopathogenic bacteria bacillus thuringiensis cry gene has reduced the damage created by insect pests in several economically important cultures. for this study, we have conducted genetic transformation of the indica rice "irga 424", via agrobacterium tumefaciens, using the b. thuringiensis cry1aa and cry1b genes, with the objective of obtaining rice plants resistant to the insect pests from this culture. the gene constructions harbor the promoters maize proteinase inh ... | 2013 | 24498761 |
| recombinant plants provide a new approach to the production of bacterial polysaccharide for vaccines. | bacterial polysaccharides have numerous clinical or industrial uses. recombinant plants could offer the possibility of producing bacterial polysaccharides on a large scale and free of contaminating bacterial toxins and antigens. we investigated the feasibility of this proposal by cloning and expressing the gene for the type 3 synthase (cps3s) of streptococcus pneumoniae in nicotinia tabacum, using the pcambia2301 vector and agrobacterium tumefaciens-mediated gene transfer. in planta the recombin ... | 2014 | 24498433 |
| agrobacterium-mediated genetic transformation and plant regeneration of the hardwood tree species fraxinus profunda. | this transformation and regeneration protocol provides an integral framework for the genetic improvement of fraxinus profunda (pumpkin ash) for future development of plants resistant to the emerald ash borer. using mature hypocotyls as the initial explants, an agrobacterium tumefaciens-mediated genetic transformation system was successfully developed for pumpkin ash (fraxinus profunda). this transformation protocol is an invaluable tool to combat the highly aggressive, non-native emerald ash bor ... | 2014 | 24493252 |
| overexpression of rice nac gene snac1 improves drought and salt tolerance by enhancing root development and reducing transpiration rate in transgenic cotton. | the snac1 gene belongs to the stress-related nac superfamily of transcription factors. it was identified from rice and overexpressed in cotton cultivar yz1 by agrobacterium tumefaciens-mediated transformation. snac1-overexpressing cotton plants showed more vigorous growth, especially in terms of root development, than the wild-type plants in the presence of 250 mm nacl under hydroponic growth conditions. the content of proline was enhanced but the mda content was decreased in the transgenic cott ... | 2014 | 24489802 |
| quorum vs. diffusion sensing: a quantitative analysis of the relevance of absorbing or reflecting boundaries. | the consequences of the boundary conditions (signal reflecting vs. signal adsorbing) on bacterial intercellular communication were addressed by a combined physics and microbiology approach. a predictive biophysical model was devised that considered system size, diffusion from given points, signal molecule decay and boundary properties. the theoretical predictions were tested with two experimental agarose-gel-based set-ups for reflecting or absorbing boundaries. n-acyl homoserine lactone (ahl) co ... | 2014 | 24484313 |
| transient down-regulation of the rna silencing machinery increases efficiency of agrobacterium-mediated transformation of arabidopsis. | agrobacterium tumefaciens is a plant pathogen that is widely used in plant transformation. as the process of transgenesis includes the delivery of single-stranded t-dna molecule, we hypothesized that transformation rate may negatively correlate with the efficiency of the rna-silencing machinery. using mutants compromised in either the transcriptional or post-transcriptional gene-silencing pathways, two inhibitors of stable transformation were revealed-ago2 and nrpd1a. furthermore, an immunopreci ... | 2014 | 24472037 |
| physiological and photosynthetic characteristics of indica hang2 expressing the sugarcane pepc gene. | phosphoenolpyruvate carboxylase (pepc) is known to play a key role in the initial fixation of co2 in c4 photosynthesis. the pepc gene from sugarcane (a c4 plant) was introduced into indica rice (hang2), a process mediated by agrobacterium tumefaciens. integration patterns and copy numbers of the gene was confirmed by dna blot analysis. rt-pcr and western blotting results showed that the pepc gene was expressed at both the mrna and protein levels in the transgenic lines. real-time pcr results ind ... | 2014 | 24469712 |
| enhanced resistance to blast fungus in rice (oryza sativa l.) by expressing the ribosome-inactivating protein α-momorcharin. | rice blast caused by magnaporthe grisea is one of the three major diseases that seriously affect the rice production. alpha-momorcharin (α-mc), a ribosome-inactivating protein (rip) isolated from momordica charantia seeds, has antifungal effects in vitro. in this study, the α-mc gene was constitutively expressed under the control of the 2×35s promoter in transgenic rice (oryza sativa l.) using an agrobacterium tumefaciens-mediated method. the nine transgenic plants were obtained and confirmed by ... | 2014 | 24467890 |
| overexpression of ptsos2 enhances salt tolerance in transgenic poplars. | protein kinases are major signal transduction factors that have a central role in mediating acclimation to environmental changes in eukaryotic organisms. in this study, we cloned and identified three salt overly sensitive 2 (sos2) genes in the woody plant populus trichocarpa, designated as ptsos2.1, ptsos2.2, and ptsos2.3, which were transformed into hybrid poplar clone t89 (populus tremula× populus tremuloides michx clone t89) mediated by agrobacterium tumefaciens. southern and northern blot an ... | 2014 | 24465084 |
| identification and characterization of a second quorum-sensing system in agrobacterium tumefaciens a6. | quorum sensing (qs) is a widespread mechanism of bacterial communication in which individual cells produce and respond to small chemical signals. in agrobacterium tumefaciens, an acylhomoserine lactone-dependent qs mechanism is known to regulate the replication and conjugation of the tumor-inducing (ti) plasmid. most of the qs regulatory proteins are encoded within the ti plasmid. among them, trai is the luxi-type enzyme synthesizing the qs signal n-3-oxooctanoyl-l-homoserine lactone (3oc8hsl), ... | 2014 | 24464459 |
| an efficient tool for random insertional mutagenesis: agrobacterium tumefaciens-mediated transformation of the filamentous fungus aspergillus terreus. | agrobacterium tumefaciens-mediated transformation (atmt) has been widely used in various organisms. in the current study, we developed a simple and efficient system for genetic transformation of the filamentous fungus aspergillus terreus using atmt. the transformation protocol was optimized for certain parameters to rapidly generate a library of transferred dna (t-dna) insertion mutants of a. terreus. the presence of mitotically stable hygromycin resistance gene (hph) integration in the genome w ... | 2014 | 24462974 |
| involvement of rad52 in t-dna circle formation during agrobacterium tumefaciens-mediated transformation of saccharomyces cerevisiae. | agrobacterium tumefaciens cells carrying a tumour inducing plasmid (ti-plasmid) can transfer a defined region of transfer dna (t-dna) to plant cells as well as to yeast. this process of agrobacterium-mediated transformation (amt) eventually results in the incorporation of the t-dna in the genomic dna of the recipient cells. all available evidence indicates that t-strand transfer closely resembles conjugal dna transfer as found between gram-negative bacteria. however, where conjugal plasmid dna t ... | 2014 | 24460832 |
| galactaro δ-lactone isomerase: lactone isomerization by a member of the amidohydrolase superfamily. | agrobacterium tumefaciens strain c58 can utilize d-galacturonate as a sole source of carbon via a pathway in which the first step is oxidation of d-galacturonate to d-galactaro-1,5-lactone. we have identified a novel enzyme, d-galactarolactone isomerase (gli), that catalyzes the isomerizaton of d-galactaro-1,5-lactone to d-galactaro-1,4-lactone. gli, a member of the functionally diverse amidohydrolase superfamily, is a homologue of ligi that catalyzes the hydrolysis of 2-pyrone-4,6-dicarboxylate ... | 2014 | 24450804 |
| use of opda, an organophosphorus (op) hydrolase, prevents lethality in an african green monkey model of acute op poisoning. | organophosphorus (op) pesticides are a diverse class of acetylcholinesterase (ache) inhibitors that are responsible for tremendous morbidity and mortality worldwide, killing approximately 300,000 people annually. enzymatic hydrolysis of ops is a potential therapy for acute poisoning. opda, an op hydrolase isolated from agrobacterium radiobacter, has been shown to decrease lethality in rodent models of op poisoning. this study investigated the effects of opda on ache activity, plasma concentratio ... | 2014 | 24447378 |
| detection of an o-methyltransferase synthesising acetosyringone in methyl jasmonate-treated tobacco cell-suspensions cultures. | acetosyringone (3',5'-dimethoxy-4'-hydroxyacetophenone) is a well-known and very effective inducer of the virulence genes of agrobacterium tumefaciens but the precise pathway of its biosynthesis in plants is still unknown. we have used two tobacco cell lines, cultured in suspension and exhibiting different patterns of accumulation of acetosyringone in their culture medium upon treatment with methyl jasmonate, to study different steps of acetosyringone biosynthesis. in the two cell lines studied, ... | 2014 | 24445177 |
| a signaling pathway involving the diguanylate cyclase celr and the response regulator divk controls cellulose synthesis in agrobacterium tumefaciens. | the production of cellulose fibrils is involved in the attachment of agrobacterium tumefaciens to its plant host. consistent with previous studies, we reported recently that a putative diguanylate cyclase, celr, is required for synthesis of this polymer in a. tumefaciens. in this study, the effects of celr and other components of the regulatory pathway of cellulose production were explored. mutational analysis of celr demonstrated that the cyclase requires the catalytic ggeef motif, as well as t ... | 2014 | 24443526 |
| dna replicons for plant genome engineering. | sequence-specific nucleases enable facile editing of higher eukaryotic genomic dna; however, targeted modification of plant genomes remains challenging due to ineffective methods for delivering reagents for genome engineering to plant cells. here, we use geminivirus-based replicons for transient expression of sequence-specific nucleases (zinc-finger nucleases, transcription activator-like effector nucleases, and the clustered, regularly interspaced, short palindromic repeat/cas system) and deliv ... | 2014 | 24443519 |
| retracted article: agrobacterium tumefaciens-mediated transformation and efficient regeneration of a timber yielding plant dalbergia sissoo roxb. | 2013 | 24431531 | |
| agroinfiltration and pvx agroinfection in potato and nicotiana benthamiana. | agroinfiltration and pvx agroinfection are two efficient transient expression assays for functional analysis of candidate genes in plants. the most commonly used agent for agroinfiltration is agrobacterium tumefaciens, a pathogen of many dicot plant species. this implies that agroinfiltration can be applied to many plant species. here, we present our protocols and expected results when applying these methods to the potato (solanum tuberosum), its related wild tuber-bearing solanum species (solan ... | 2014 | 24430891 |
| bcpmi2, isolated from non-heading chinese cabbage encoding phosphomannose isomerase, improves stress tolerance in transgenic tobacco. | phosphomannose isomerase (pmi) is an enzyme that catalyses the first step of the l-galactose pathway for ascorbic acid (asa) biosynthesis in plants. to clarify the physiological roles of pmi in asa biosynthesis, the cdna sequence of pmi was cloned from non-heading chinese cabbage (brassica campestris ssp. chinensis makino) and overexpressed in tobacco transformed with agrobacterium tumefaciens. the asa and soluble sugar contents were lower in 35s::bcpmi2 tobacco than in wild-type tobacco. howeve ... | 2014 | 24430300 |
| in vitro antibacterial activity of cysteine protease inhibitor from kiwifruit (actinidia deliciosa). | the need for replacing traditional pesticides with alternative agents for the management of agricultural pathogens is rising worldwide. in this study, a cysteine proteinase inhibitor (cpi), 11 kda in size, was purified from green kiwifruit to homogeneity. we examined the growth inhibition of three plant pathogenic gram-negative bacterial strains by kiwi cpi and attempted to elucidate the potential mechanism of the growth inhibition. cpi influenced the growth of phytopathogenic bacteria agrobacte ... | 2012 | 24426085 |
| the involvement of the 3':5'-cyclic-amp phosphodiesterase in transformation and growth of crown-gall tumors in bryophyllum daigremontianum. | in crown-gall tumor tissue obtained from leaves of bryophyllum daigremontianum an adenosine 3':5'-cyclic phosphate (3':5'-cyclic-amp) degrading activity increases up to 2.5 fold until the fifth day after inoculation with agrobacterium tumefaciens, declining to the value of the control in the solid tumor. theophylline up to 1 mmol l(-1) given to wounded leaves of bryophyllum daigremontianum has no effect on the number of tumors. the effect of higher concentrations given over extended periods can ... | 1977 | 24420634 |
| purification, crystallization and preliminary x-ray diffraction analysis of a novel keto-deoxy-d-galactarate (kdg) dehydratase from agrobacterium tumefaciens. | d-galacturonic acid is the main component of pectin. it could be used to produce affordable renewable fuels, chemicals and materials through biotechnical conversion. keto-deoxy-d-galactarate (kdg) dehydratase is an enzyme in the oxidative pathway of d-galacturonic acid in agrobacterium tumefaciens (at). it converts 3-deoxy-2-keto-l-threo-hexarate to α-ketoglutaric semialdehyde. at kdg dehydratase was crystallized by the hanging-drop vapour-diffusion method. the crystals belonged to the monoclini ... | 2013 | 24419616 |
| technical communication: a simple and economically viable medium for the growth of agrobacterium radiobacter for the production of d-amino acid. | a simple and cost effective medium for the growth and d-amino acid production by agrobacterium radiobacter from hydantoin is reported. the effectiveness of this medium is compared with other synthetic media. | 1996 | 24415435 |
| unlocking the potential of tropical root crop biotechnology in east africa by establishing a genetic transformation platform for local farmer-preferred cassava cultivars. | cassava genetic transformation capacity is still mostly restricted to advanced laboratories in the usa, europe and china; and its implementation and maintenance in african laboratories has remained scarce. the impact of transgenic technologies for genetic improvement of cassava will depend largely on the transfer of such capabilities to researchers in africa, where cassava has an important socioeconomic niche. a major constraint to the development of genetic transformation technologies for cassa ... | 2013 | 24400011 |
| characterization of rhizobium grahamii extrachromosomal replicons and their transfer among rhizobia. | rhizobium grahamii belongs to a new phylogenetic group of rhizobia together with rhizobium mesoamericanum and other species. r. grahamii has a broad-host-range that includes leucaena leucocephala and phaseolus vulgaris, although it is a poor competitor for p. vulgaris nodulation in the presence of rhizobium etli or rhizobium phaseoli strains. this work analyzed the genome sequence and transfer properties of r. grahamii plasmids. | 2014 | 24397311 |
| agrobacterium infection and plant defense-transformation success hangs by a thread. | the value of agrobacterium tumefaciens for plant molecular biologists cannot be appreciated enough. this soil-borne pathogen has the unique capability to transfer dna (t-dna) into plant systems. gene transfer involves both bacterial and host factors, and it is the orchestration of these factors that determines the success of transformation. some plant species readily accept integration of foreign dna, while others are recalcitrant. the timing and intensity of the microbially activated host defen ... | 2013 | 24391655 |
| mechanism and structure of the bacterial type iv secretion systems. | the bacterial type iv secretion systems (t4sss) translocate dna and protein substrates to bacterial or eukaryotic target cells generally by a mechanism dependent on direct cell-to-cell contact. the t4sss encompass two large subfamilies, the conjugation systems and the effector translocators. the conjugation systems mediate interbacterial dna transfer and are responsible for the rapid dissemination of antibiotic resistance genes and virulence determinants in clinical settings. the effector transl ... | 2014 | 24389247 |
| molecular strategies to replace the structural metal site in the prokaryotic zinc finger domain. | the specific arrangement of secondary elements in a local motif often totally relies on the formation of coordination bonds between metal ions and protein ligands. this is typified by the ~30 amino acid eukaryotic zinc finger motif in which a β-sheet and an α-helix are clustered around a zinc ion by various combinations of four ligands. the prokaryotic zinc finger domain (found in the ros protein from agrobacterium tumefaciens) is different from the eukaryotic counterpart as it consists of 58 am ... | 2014 | 24389235 |
| agrobacterium tumefaciens-mediated transformation of the lichen fungus, umbilicaria muehlenbergii. | transformation-mediated mutagenesis in both targeted and random manners has been widely applied to decipher gene function in diverse fungi. however, a transformation system has not yet been established for lichen fungi, severely limiting our ability to study their biology and mechanism underpinning symbiosis via gene manipulation. here, we report the first successful transformation of the lichen fungus, umbilicaria muehlenbergii, via the use of agrobacterium tumefaciens. we generated a total of ... | 2013 | 24386304 |
| agrobacterium tumefaciens-mediated transformation of valsa mali: an efficient tool for random insertion mutagenesis. | valsa mali is a causal agent of apple and pear trees canker disease, which is a destructive disease that causes serious economic losses in eastern asia, especially in china. the lack of an efficient transformation system for valsa mali retards its investigation, which poses difficulties to control the disease. in this research, a transformation system for this pathogen was established for the first time using a. tumefaciens-mediated transformation (atmt), with the optimal transformation conditio ... | 2013 | 24381526 |
| at a supra-physiological concentration, human sexual hormones act as quorum-sensing inhibitors. | n-acylhomoserine lactone (ahl)-mediated quorum-sensing (qs) regulates virulence functions in plant and animal pathogens such as agrobacterium tumefaciens and pseudomonas aeruginosa. a chemolibrary of more than 3500 compounds was screened using two bacterial ahl-biosensors to identify qs-inhibitors (qsis). the purity and structure of 15 qsis selected through this screening were verified using hplc ms/ms tools and their activity tested on the a. tumefaciens and p. aeruginosa bacterial models. the ... | 2013 | 24376718 |
| visualization of vire2 protein translocation by the agrobacterium type iv secretion system into host cells. | type iv secretion systems (t4ss) can mediate the translocation of bacterial virulence proteins into host cells. the plant pathogen agrobacterium tumefaciens uses a t4ss to deliver a vird2-single stranded dna complex as well as the virulence proteins vird5, vire2, vire3, and virf into host cells so that these become genetically transformed. besides plant cells, yeast and fungi can efficiently be transformed by agrobacterium. translocation of virulence proteins by the t4ss has so far only been sho ... | 2013 | 24376037 |
| two fatty acid desaturases, stearoyl-acyl carrier protein δ9-desaturase6 and fatty acid desaturase3, are involved in drought and hypoxia stress signaling in arabidopsis crown galls. | agrobacterium tumefaciens-derived crown galls of arabidopsis (arabidopsis thaliana) contain elevated levels of unsaturated fatty acids and strongly express two fatty acid desaturase genes, ω3 fatty acid desaturase3 (fad3) and stearoyl-acyl carrier protein δ9-desaturase6 (sad6). the fad3-2 mutant with impaired α-linolenic acid synthesis developed significantly smaller crown galls under normal, but not under high, relative humidity. this strongly suggests that fad3 plays a role in increasing droug ... | 2014 | 24368335 |
| quorum-dependent mannopine-inducible conjugative transfer of an agrobacterium opine-catabolic plasmid. | the ti plasmid in agrobacterium tumefaciens strain 15955 carries two alleles of trar that regulate conjugative transfer. the first is a functional allele, called trar, that is transcriptionally induced by the opine octopine. the second, trlr, is a nonfunctional, dominant-negative mutant located in an operon that is inducible by the opine mannopine (mop). based on these findings, we predicted that there exist wild-type agrobacterial strains harboring plasmids in which mop induces a functional tra ... | 2014 | 24363349 |
| identification of functionally important conserved trans-membrane residues of bacterial pib -type atpases. | powered by atp hydrolysis, p(ib) -atpases drive the energetically uphill transport of transition metals. these high affinity pumps are essential for heavy metal detoxification and delivery of metal cofactors to specific cellular compartments. amino acid sequence alignment of the trans-membrane (tm) helices of p(ib)-atpases reveals a high degree of conservation, with ∼ 60-70 fully conserved positions. of these conserved positions, 6-7 were previously identified to be important for transport. howe ... | 2014 | 24350798 |
| cloning and characterization of a novel dehydrin gene, sidhn2, from saussurea involucrata kar. et kir. | saussurea involucrata kar. et kir. is a hardy dicotyledonous plant capable of tolerating severe abiotic stress conditions. in a previous study, we created a cdna library to determine what factors are associated with the cold acclimation response in s. involucrata. from this, a full-length cdna of a dehydrin-like gene (sidhn2) was obtained by rt-pcr. the sidhn2 gene was characterized in this study. the full-length sidhn2 cdna comprised 693 bp containing an open reading frame of 345 bp specifying ... | 2014 | 24337866 |
| maize (zea mays l.) transformation by agrobacterium tumefaciens infection of pollinated ovules. | a novel transformation system was established for maize using agrobacterium infection of in vitro cultured ovules. the maize ovules were isolated 24h after pollination and infected with agrobacterium. the embryos were isolated from the pollinated ovules 2-3 weeks after agrobacterium infection, regenerated to plantlets and investigated for transgene expression and inheritance. experimental evaluations were focused on the four main aspects. firstly, through the introduction of gus gene for monitor ... | 2014 | 24333124 |
| field performance of transgenic sugarcane produced using agrobacterium and biolistics methods. | future genetic improvement of sugarcane depends, in part, on the ability to produce high-yielding transgenic cultivars with improved traits such as herbicide and insect resistance. here, transgenic sugarcane plants generated by different transformation methods were assessed for field performance over 3 years. agrobacterium-mediated (agro) transgenic events (35) were produced using four different agrobacterium tumefaciens strains, while biolistic (biol) transgenic events (48) were produced using ... | 2014 | 24330327 |
| studies on the structure of cointegrates between octopine and nopaline ti-plasmids and their tumour-inducing properties. | stable cointegrates between incrh-1 octopine (ach5) and nopaline (c58) ti-plasmids, present in ten independently isolated agrobacterium tumefaciens strains, showed identical restriction endonuclease patterns. each cointegration event had taken place in the common sequence between the t-regions of both ti-plasmids. this illustrates a high preference for this region when used in the formation of cointegrates. four crown gall tissues, obtained after transformation of nicotiana tabacum cells by one ... | 1982 | 24318033 |
| double infection of tobacco plants by two complementing octopine t-region mutants of agrobacterium tumefaciens. | the molecular basis of complementation by a mixture of two different types of octopine t-region mutants (lba4060 and lba4210) was studied. six randomly chosen cellular clones derived from a tumor obtained after mixed infection were analyzed for their t-dna content via southern blot hybridization. the clones appeared to contain t-dna that originated from each of both mutants, indicating that they developed from doubly infected single cells. genetic complementation, therefore, might explain at lea ... | 1982 | 24317964 |
| the lysopinedehydrogenase gene used as a marker for the selection of octopine crown gall cells. | plant cells transformed into octopine-synthesizing tumour cells by the bacterium agrobacterium tumefaciens survive when cultured in the presence of homo-arginine (ha), whereas both normal plant cells and nopaline producing plant tumour cells do not. survival of octopine crown gall cells is due to the activity of the enzyme lysopinedehydrogenase (lpdh) in these cells, which converts toxic homo-arginine into non-toxic homo-octopine. the selective toxicity of homo-arginine for normal cells can be a ... | 1982 | 24317894 |
| the fusarium oxysporum effector six6 contributes to virulence and suppresses i-2-mediated cell death. | plant pathogens secrete effectors to manipulate their host and facilitate colonization. fusarium oxysporum f. sp. lycopersici is the causal agent of fusarium wilt disease in tomato. upon infection, f. oxysporum f. sp. lycopersici secretes numerous small proteins into the xylem sap (six proteins). most six proteins are unique to f. oxysporum, but six6 is an exception; a homolog is also present in two colletotrichum spp. six6 expression was found to require living host cells and a knockout of six6 ... | 2014 | 24313955 |
| structural insight into l-ribulose 3-epimerase from mesorhizobium loti. | l-ribulose 3-epimerase (l-re) from mesorhizobium loti has been identified as the first ketose 3-epimerase that shows the highest observed activity towards ketopentoses. in the present study, the crystal structure of the enzyme was determined to 2.7 å resolution. the asymmetric unit contained two homotetramers with the monomer folded into an (α/β)8-barrel carrying four additional short α-helices. the overall structure of m. loti l-re showed significant similarity to the structures of ketose 3-epi ... | 2013 | 24311575 |
| generation of selectable marker-free transgenic eggplant resistant to alternaria solani using the r/rs site-specific recombination system. | marker-free transgenic eggplants, exhibiting enhanced resistance to alternaria solani , can be generated on plant growth regulators (pgrs)- and antibiotic-free ms medium employing the multi-auto-transformation (mat) vector, pmat21 - wasabi defensin , wherein isopentenyl transferase ( ipt ) gene is used as a positive selection marker. | 2014 | 24311155 |
| tumor formation on plants by mixtures of attenuated agrobacterium tumefaciens t-dna mutants. | the inoculation of plants with mixtures consisting of an a. tumefaciens mutant with a mutation in the auxin-locus of the t-dna and a mutant with a mutation in the cytokinin-locus of the t-dna invariably led to full tumor induction, in spite of the low oncogenicity of each of the mutants when inoculated separately. a similar complementation was observed when mixtures of certain auxin-locus mutants were used for infection. in these experiments the auxin-locus mutants fell into two classes. one cla ... | 1984 | 24310566 |
| silent t-dna genes in plant lines transformed by agrobacterium tumefaciens are activated by grafting and by 5-azacytidine treatment. | a shooty tumor induced by a shooter mutant of an octopine strain of agrobacterium tumefaciens was cloned. one clone obtained (ts038) behaved aberrantly in that it grew as a shooty tumor tissue on phytohormone free medium, but did not contain octopine synthase activity. in line ts038 the genes for octopine synthase and for the enzymes involved in agropine and mannopine synthesis were present, but were not transcribed. however, the above genes became active in ts038 tumor shoots after grafting as ... | 1984 | 24310565 |
| stable transformation and reverse genetic analysis of penium margaritaceum: a platform for studies of charophyte green algae, the immediate ancestors of land plants. | the charophyte green algae (cga, streptophyta, viridiplantae) occupy a key phylogenetic position as the immediate ancestors of land plants but, paradoxically, are less well-studied than the other major plant lineages. this is particularly true in the context of functional genomic studies, where the lack of an efficient protocol for their stable genetic transformation has been a major obstacle. observations of extant cga species suggest the existence of some of the evolutionary adaptations that h ... | 2014 | 24308430 |
| a disarmed binary vector from agrobacterium tumefaciens functions in agrobacterium rhizogenes : frequent co-transformation of two distinct t-dnas. | binary ti plasmid vector systems consist of two plasmids in agrobacterium, where one plasmid contains the dna that can be transferred to plant cells and the other contains the virulence (vir) genes which are necessary for the dna transfer but are not themselves stably transferred. we have constructed two nononcogenic vectors (parc4 and parc8) based on the binary ti plasmid system of agrobacterium tumefaciens for plant transformation. each vector contains the left and right termini sequences from ... | 1986 | 24307418 |
| potato spindle tuber viroid infections mediated by the ti plasmid of agrobacterium tumefaciens. | full length copies of potato spindle tuber viroid (pstv) were introduced into plant cells using an agrobacterium tumefaciens vector. crown galls containing the pstv dna were induced on tomato plants, and the plants analysed for systemic replication of the viroid. two separately derived multimeric pstv insertions in the t-dna were infectious on every plant inoculated. however, monomeric pstv gave rise to significant levels of infection only when an adjacent plant promoter could direct transcripti ... | 1986 | 24307321 |
| changes in translatable poly(a) rna from differentiated potato tissues transformed with shoot-inducing ti tl-dna of agrobacterium tumefaciens. | two dimensional gel electrophoresis was used to examine differences in steady state total poly(a) rna from untransformed potato (solanum tuberosum cv. maris bard) and potato transformed with shoot-inducing tl-dna from a. tumefaciens. rna was compared from phenotypically very distinct in vitro cultured shoots, more similar grafted plants and tubers. in each case between 200-400 translation products were identified representing the more abundant poly(a) mrna's. in general, poly(a) rna from the tra ... | 1986 | 24307320 |
| site-specific mutagenesis of potato spindle tuber viroid cdna: : alterations within premelting region 2 that abolish infectivity. | the infectivity of cloned viroid cdnas permits investigation of structure/function relationships in these unusual pathogenic rnas by systematic site-specific mutagenesis of the cdnas and subsequent bioassay. we have used three different strategies to create nucleotide substitutions within premelting region 2, a region of potato spindle tuber viroid (pstv) believed to be important in viroid replication: sodium bisulfitecatalyzed deamination of deoxycytosine residues, oligonucleotide-directed muta ... | 1986 | 24307277 |
| independent integration and seed-transmission of the tr-dna of the octopine ti plasmid pti ach5 in nicotiana plumbaginifolia. | after co-cultivation of diploid nicotiana plumbaginifolia protoplasts with an octopine-type agrobacterium tumefaciens strain (lba 4013) putative transformants were selected for hormone-independent growth, and were tested for t-dna markers. the number of transformants expressing only tl-dna markers, i.e. phytohormone autotrophy and octopine synthase, was an order of magnitude higher than that of the cell lines which were simultaneously positive for both tl- and tr-dna markers (the latter being ma ... | 1986 | 24307226 |
| structure and expression of dna transferred to tobacco via transformation of protoplasts with ti-plasmid dna: co-transfer of t-dna and non t-dna sequences. | the t-dna structure and organization in tissues obtained via transformation of tobacco protoplasts with ti-plasmid dna was found to be completely different from the t-dna introduced via agrobacterium tumefaciens. it is often fragmented. overlapping copies of t-dna, having various sizes, as well as separated fragments of t-dna were detected. the border sequences of 23 basepairs (bp), flanking the t-region in the ti-plasmid as direct repeats are not used as preferred sequences for integration. sim ... | 1985 | 24306763 |