Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| change is good: variations in common biological mechanisms in the epsilonproteobacterial genera campylobacter and helicobacter. | microbial evolution and subsequent species diversification enable bacterial organisms to perform common biological processes by a variety of means. the epsilonproteobacteria are a diverse class of prokaryotes that thrive in diverse habitats. many of these environmental niches are labeled as extreme, whereas other niches include various sites within human, animal, and insect hosts. some epsilonproteobacteria, such as campylobacter jejuni and helicobacter pylori, are common pathogens of humans tha ... | 2011 | 21372321 |
| characterization of campylobacter jejuni and campylobacter coli strains isolated in the region of nis, serbia. | campylobacter jejuni and campylobacter coli represent one of the main causes of bacterial diarrhoea in humans. although the disease is usually mild and self-limiting, severe chronic sequelae may occur, such as reactive arthritis, guillain-barré and miller fisher syndromes. serotyping is used as an epidemiological marker, while post-infective polyneuropathies are associated with several o serotypes. | 2010 | 21361150 |
| comparison of cape town and skirrow's campylobacter isolation protocols in humans and broilers in morogoro, tanzania. | comparison of cape town and skirrow's protocols used in isolation of campylobacter in humans and broilers was carried out in a cross-sectional study in morogoro, tanzania. a total of 176 and 158 human stool and broiler intestinal samples were collected, respectively. while human stool samples were collected from selected health centers, broiler intestinal samples were obtained from selected farms and chicken markets. samples were inoculated and cultured in duplicate using two protocols and preva ... | 2011 | 21359592 |
| high prevalence of intestinal carriage of campylobacter coli in patients with primary antibody deficiencies: a silent infection that could shift to a life-threatening condition. | 2011 | 21358549 | |
| pulsed field gel electrophoresis typing of human and retail foodstuff campylobacters: an irish perspective. | campylobacter enteritis is a zoonosis, an infectious disease transmissible under normal conditions from vertebrate animals to man, presenting a major global public health burden. in this study, pulsed field gel electrophoresis (pfge) was employed to identify common genotypes in a collection of 600 campylobacter isolates in order to investigate if profiles obtained from retail samples of foodstuffs matched genotypes causing illness in the community in ireland. the campylobacters were isolated fro ... | 2010 | 21356447 |
| antimicrobial resistance in campylobacter coli isolated from pigs in two provinces of china. | the aim of this study was to determine the prevalence, antimicrobial resistance and molecular epidemiology of campylobacter coli isolated from swine in china. a total of 190 c. coli isolates obtained from two slaughter houses and ten conventional pig farms in shandong (sd, n=95) and ningxia (nx, n=95) provinces were tested for their susceptibility to 14 antimicrobials. a high prevalence (>95%) of ciprofloxacin and tetracycline-resistant strains was observed in both sd and nx. the erythromycin an ... | 2011 | 21349598 |
| detection and genotypic differentiation of campylobacter jejuni and campylobacter coli strains from laying hens by multiplex pcr and fla-typing. | in total, 26 campylobacter (c.) strains, isolated from liver, spleen, caecal or jejunal content of laying hens from different flocks were examined. in these flocks a drop in egg production, an increasing mortality and livers with whitish-grey lesions as post-mortem finding were observed. suspected campylobacter colonies were differentiated using a modified m-pcr in 13 campylobacter jejuni and 13 campylobacter coli strains. all isolates were characterised by typing of the flaa and flab gene each ... | 2011 | 21349563 |
| evolution of the oligopeptide transporter family. | the oligopeptide transporter (opt) family of peptide and iron-siderophore transporters includes members from both prokaryotes and eukaryotes but with restricted distribution in the latter domain. eukaryotic members were found only in fungi and plants with a single slime mold homologue clustering with the fungal proteins. all functionally characterized eukaryotic peptide transporters segregate from the known iron-siderophore transporters on a phylogenetic tree. prokaryotic members are widespread, ... | 2011 | 21347612 |
| a charcoal- and blood-free enrichment broth for isolation and pcr detection of campylobacter jejuni and campylobacter coli in chicken. | the microaerophilic nature of campylobacter and its requirement of ~5% o(2) for growth have complicated its recovery from foods. the addition to the enrichment media of oxygen quenchers such as charcoal or blood could interfere with pcr for its detection. in this study, a two-step simple aerobic method for campylobacter detection is proposed. a modification of the tran blood-free enrichment broth (bfeb), in which charcoal was excluded from the medium (m-bfeb), was compared with the original form ... | 2011 | 21333141 |
| prevalence of putative virulence markers in campylobacter jejuni and campylobacter coli isolated from hospitalized children, raw chicken, and raw beef in tehran, iran. | the incidence of the virulence-associated genes cdta, cdtb, cdtc, cadf, dnaj, racr, and plda has been investigated in campylobacter jejuni and campylobacter coli collected from raw chicken and beef from retailers in tehran, iran, and from hospitalized children (age, =14 years) suffering from diarrhea. campylobacter spp. were collectively identified by morphological and biochemical methods. campylobacter jejuni and c. coli were discriminated from other campylobacter spp. by amplification of a spe ... | 2011 | 21326356 |
| a novel immunity system for bacterial nucleic acid degrading toxins and its recruitment in various eukaryotic and dna viral systems. | the use of nucleases as toxins for defense, offense or addiction of selfish elements is widely encountered across all life forms. using sensitive sequence profile analysis methods, we characterize a novel superfamily (the sukh superfamily) that unites a diverse group of proteins including smi1/knr4, pgs2, fbxo3, skip16, syd, herpesviral us22, irs1 and trs1, and their bacterial homologs. using contextual analysis we present evidence that the bacterial members of this superfamily are potential imm ... | 2011 | 21306995 |
| role of the pjm1 plasmid-encoded transport proteins fatb, c and d in ferric anguibactin uptake in the fish pathogen vibrio anguillarum. | vibrio anguillarum serotype o1 is part of the natural flora in the aquatic habitat, but under certain circumstances it can cause terminal haemorrhagic septicemia in marine and fresh water fish due to the action of the anguibactin iron uptake system encoded by the virulence plasmid pjm1. this plasmid harbours the genes for the biosynthesis of the siderophore anguibactin and the ferric anguibactin transport proteins fatd, c, b and a encoded in the iron transport operon. the fata protein is the out ... | 2010 | 21304833 |
| epidemiological association of different campylobacter jejuni groups with metabolism-associated genetic markers. | in this study, multilocus sequence typing (mlst) was combined with the genetic detection of six genetic markers, ansb, dmsa, ggt, cj1585c, cjj81176-1367/71 (cj1365c), and the two-gene marker tlp7 (cj0951c plus cj0952c), to assess if their presence correlated with different c. jejuni clonal groups. using a collection of 266 c. jejuni isolates from (in decreasing order of sample size) humans, chickens, cattle, and turkeys, it was further investigated whether the resulting genotypes correlated with ... | 2011 | 21278270 |
| campylobacter troglodytis sp. nov., isolated from feces of human-habituated wild chimpanzees (pan troglodytes schweinfurthii) in tanzania. | the transmission of simian immunodeficiency and ebola viruses to humans in recent years has heightened awareness of the public health significance of zoonotic diseases of primate origin, particularly from chimpanzees. in this study, we analyzed 71 fecal samples collected from 2 different wild chimpanzee (pan troglodytes) populations with different histories in relation to their proximity to humans. campylobacter spp. were detected by culture in 19/56 (34%) group 1 (human habituated for research ... | 2011 | 21278267 |
| neurologic symptoms associated with raising poultry and swine among participants in the agricultural health study. | guillain-barré syndrome (gbs) is the leading cause of acute peripheral neuropathy worldwide, often associated with recent foodborne infection with campylobacter jejuni. in this cross-sectional analysis of data from the agricultural health study, we tested whether swine and poultry exposure were associated with increased prevalence of gbs-like neurologic symptoms. | 2011 | 21270654 |
| microbial diagnostic microarray for food- and water-borne pathogens. | a microbial diagnostic microarray for the detection of the most relevant bacterial food- and water-borne pathogens and indicator organisms was developed and thoroughly validated. the microarray platform based on sequence-specific end labelling of oligonucleotides and the pyhylogenetically robust gyrb marker gene allowed a highly specific (resolution on genus/species level) and sensitive (0.1% relative and 10(4) cfu absolute detection sensitivity) detection of the target pathogens. validation was ... | 2010 | 21255342 |
| persistence of free-living protozoan communities across rearing cycles in commercial poultry houses. | the introduction and survival of zoonotic bacterial pathogens in poultry farming have been linked to bacterial association with free-living protozoa. to date, however, no information is available on the persistence of protozoan communities in these environments across consecutive rearing cycles and how it is affected by farm- and habitat-specific characteristics and management strategies. we therefore investigated the spatial and temporal dynamics of free-living protozoa in three habitats (pipel ... | 2011 | 21239551 |
| survival of escherichia coli, enterococci, and campylobacter spp. in sheep feces on pastures. | the survival of enteric bacteria in 10 freshly collected sheep fecal samples on pastures was measured in each of four seasons. ten freshly collected feces were placed on pasture, and concentrations of escherichia coli, enterococci, and campylobacter spp. were monitored until exhaustion of the fecal samples. in all four seasons, there was an increase in enterococcal concentrations by up to 3 orders of magnitude, with peak concentrations recorded between 11 and 28 days after deposition. e. coli co ... | 2011 | 21239546 |
| taxonomy, epidemiology, and clinical relevance of the genus arcobacter. | the genus arcobacter, defined almost 20 years ago from members of the genus campylobacter, has become increasingly important because its members are being considered emergent enteropathogens and/or potential zoonotic agents. over recent years information that is relevant for microbiologists, especially those working in the medical and veterinary fields and in the food safety sector, has accumulated. recently, the genus has been enlarged with several new species. the complete genomes of arcobacte ... | 2011 | 21233511 |
| do patients with recurrent episodes of campylobacteriosis differ from those with a single disease event? | although campylobacter is the leading cause of reported bacterial gastro-enteritis in industrialized countries, little is known on its recurrence. the objective of this study is to describe the risk and the patient characteristics of recurrent episodes of human campylobacteriosis reported in quebec. | 2011 | 21226938 |
| adherence, invasion, toxigenic, and chemotactic properties of mexican campylobacter strains. | to determine the virulence factors of mexican wild-type strains of campylobacter jejuni and campylobacter coli, 31 wild-type strains were isolated from food and from humans. the production of cytolethal distending toxin and the adherence and invasion capabilities of these strains were assayed in vero cells. hard agar plugs with repellents and attractants were used to examine chemotaxis. mueller-hinton agar with supplements was used for motility analysis and to measure hemolytic activity. nine st ... | 2010 | 21219724 |
| in vivo recognition of the feca3 target promoter by helicobacter pylori nikr. | in helicobacter pylori, the transcriptional regulator hpnikr represses transcription of the feca3 gene by binding to two adjacent operators spanning a region of almost 80 nucleotides along the feca3 promoter in a nickel-dependent manner. by employing hydroxyl radical footprinting, we mapped the protected nucleotides within each operator. three short sequences rich in a and t nucleotides were identified within each operator, comprising just 24 bases for both operators, with 4 or 5 protected bases ... | 2011 | 21216998 |
| arcobacter population dynamics in pigs on farrow-to-finish farms. | healthy pigs are an important reservoir for the emerging human pathogen arcobacter which can result in contamination of porcine carcasses and pork and the spread of arcobacters into the environment. up to now, the excretion of arcobacters by pigs has been studied, but information about the transmission routes in fattening pigs is lacking. the present study aimed to elucidate the arcobacter population dynamics in pigs during the fattening period on four farrow-to-finish farms. on each farm, 30 cl ... | 2011 | 21216902 |
| detection, isolation, and characterization of helicobacter species from the gastrointestinal tract of the brushtail possum. | the presence of helicobacter species in australian marsupials was examined systematically using microscopy, culture, and pcr in different regions of the gastrointestinal tract (git) and in the liver of brushtail possums (btps) (trichosurus vulpecula), a common australian marsupial that feeds on eucalyptus leaves. the spatial distribution of helicobacter species in the git sections also was examined microscopically in silver-stained sections and by fluorescent in situ hybridization (fish) using a ... | 2011 | 21216901 |
| introgression in the genus campylobacter: generation and spread of mosaic alleles. | horizontal genetic exchange strongly influences the evolution of many bacteria, substantially contributing to difficulties in defining their position in taxonomic groups. in particular, how clusters of related bacterial genotypes - currently classified as microbiological species - evolve and are maintained remains controversial. the nature and magnitude of gene exchange between two closely related (approx. 15 % nucleotide divergence) microbiologically defined species, campylobacter jejuni and ca ... | 2011 | 21212120 |
| influence of tetracycline resistance on the transport of manure-derived escherichia coli in saturated porous media. | in this research, tetracycline resistant (tet(r)) and tetracycline susceptible (tet(s)) escherichia coli isolates were retrieved from dairy manure and the influence of tetracycline resistance on the transport of e. coli in saturated porous media was investigated through laboratory column transport experiments. experimental results showed that tet(r)e. coli strains had higher mobility than the tet(s) strains in saturated porous media. measurements of cell surface properties suggested that tet(r)e ... | 2010 | 21211813 |
| influence of tetracycline resistance on the transport of manure-derived escherichia coli in saturated porous media. | in this research, tetracycline resistant (tet(r)) and tetracycline susceptible (tet(s)) escherichia coli isolates were retrieved from dairy manure and the influence of tetracycline resistance on the transport of e. coli in saturated porous media was investigated through laboratory column transport experiments. experimental results showed that tet(r)e. coli strains had higher mobility than the tet(s) strains in saturated porous media. measurements of cell surface properties suggested that tet(r)e ... | 2010 | 21211813 |
| new methods for detection of campylobacters in stool samples in comparison to culture. | campylobacter species, especially campylobacter jejuni and campylobacter coli, are a major cause of human bacterial enteritis. current detection in stools is done essentially by culture on selective and nonselective media with filtration. these methods were compared to 2 molecular biology methods, an in-house real-time pcr and a multiplex pcr named seeplex diarrhea ace detection, and 3 immunoenzymatic methods, premier campy, ridascreen campylobacter, and immunocard stat!campy. out of 242 stool s ... | 2011 | 21209172 |
| successful treatment of cryptosporidiosis in 2 common marmosets (callithrix jacchus) by using paromomycin. | two pair-housed, 1-y-old common marmosets (callithrix jacchus) had intermittent loose feces and weight loss for approximately 2 mo. cryptosporidum parvum was identified by elisa in the feces of both animals. cbc and blood chemistry values, including liver enzymes, were within normal range. both marmosets were treated with the antibiotic paromomycin (15 mg/kg po) twice daily for 28 d. resolution of clinical signs coincided with treatment. three follow-up samples, taken 2 wk apart after treatment ... | 2010 | 21205457 |
| description and burden of travel-related cases caused by enteropathogens reported in a canadian community. | risk of infections by enteropathogens among individuals traveling outside their country of residence is considered important. such travel-related cases (trc) have been poorly estimated and described in canada. | 2010 | 21199137 |
| evolution of an agriculture-associated disease causing campylobacter coli clade: evidence from national surveillance data in scotland. | the common zoonotic pathogen campylobacter coli is an important cause of bacterial gastroenteritis worldwide but its evolution is incompletely understood. using multilocus sequence type (mlst) data of 7 housekeeping genes from a national survey of campylobacter in scotland (2005/6), and a combined population genetic-phylogenetics approach, we investigated the evolutionary history of c. coli. genealogical reconstruction of isolates from clinical infection, farm animals and the environment, reveal ... | 2010 | 21179537 |
| evaluation of salmonella-vectored campylobacter peptide epitopes for reduction of campylobacter jejuni in broiler chickens. | campylobacter is a leading cause of bacterial gastroenteritis in humans and is often linked to contaminated poultry products. live salmonella vectors expressing three linear peptide epitopes from campylobacter proteins cj0113 (omp18/cjad), cj0982c (cjaa), and cj0420 (ace393) were administered to chicks by oral gavage on the day of hatch, and the chicks were challenged with campylobacter jejuni on day 21. all three candidate vaccines produced consistent humoral immune responses with high levels o ... | 2010 | 21177910 |
| evaluation of salmonella-vectored campylobacter peptide epitopes for reduction of campylobacter jejuni in broiler chickens. | campylobacter is a leading cause of bacterial gastroenteritis in humans and is often linked to contaminated poultry products. live salmonella vectors expressing three linear peptide epitopes from campylobacter proteins cj0113 (omp18/cjad), cj0982c (cjaa), and cj0420 (ace393) were administered to chicks by oral gavage on the day of hatch, and the chicks were challenged with campylobacter jejuni on day 21. all three candidate vaccines produced consistent humoral immune responses with high levels o ... | 2010 | 21177910 |
| novel glycosylation sites localized in campylobacter jejuni flagellin flaa by liquid chromatography electron capture dissociation tandem mass spectrometry. | glycosylation of flagellin in campylobacter jejuni is essential for motility and virulence. it is well-known that flagellin from c. jejuni 81-176 is glycosylated by pseudaminic acid and its acetamidino derivative, and that campylobactor coli vc167 flagellin is glycosylated by legionaminic acid and its derivatives. recently, it was shown, by use of a metabolomics approach, that c. jejuni 11168 is glycosylated by dimethyl glyceric acid derivatives of pseudaminic acid, but the sites of glycosylatio ... | 2011 | 21158479 |
| novel glycosylation sites localized in campylobacter jejuni flagellin flaa by liquid chromatography electron capture dissociation tandem mass spectrometry. | glycosylation of flagellin in campylobacter jejuni is essential for motility and virulence. it is well-known that flagellin from c. jejuni 81-176 is glycosylated by pseudaminic acid and its acetamidino derivative, and that campylobactor coli vc167 flagellin is glycosylated by legionaminic acid and its derivatives. recently, it was shown, by use of a metabolomics approach, that c. jejuni 11168 is glycosylated by dimethyl glyceric acid derivatives of pseudaminic acid, but the sites of glycosylatio ... | 2011 | 21158479 |
| drinking water as the source of campylobacter coli infection in grandparent heavy breeders. | the aim of the present study was the molecular identification of a common source of infection of campylobacter coli in two grandparent breeder farms. campylobacter jejuni and c. coli were isolated from well water and cloacal swabs from grandparent chickens. colonies were genotyped using restriction fragment length polymorphism-flaa gene, pulsed field gel electrophoresis and multi-locus sequence typing. the same genotype of c. coli was found in both farms and in the well from which drinking water ... | 2010 | 21154058 |
| in vitro activity and single-step mutational analysis of rifamycin sv tested against enteropathogens associated with traveler's diarrhea and clostridium difficile. | rifamycin sv is a broad-spectrum, poorly absorbed antimicrobial agent that, when coupled with mmx technology, is being targeted for the oral treatment of traveler's diarrhea (td) and clostridium difficile-associated disease (cdad). rifamycin sv was tested for activity against 911 td-associated enteropathogens and 30 c. difficile isolates collected from several global surveillance studies. rifamycin sv demonstrated similar antimicrobial activity levels against the enterobacteriaceae, with mic₅₀ v ... | 2010 | 21149623 |
| in vitro activity and single-step mutational analysis of rifamycin sv tested against enteropathogens associated with traveler's diarrhea and clostridium difficile. | rifamycin sv is a broad-spectrum, poorly absorbed antimicrobial agent that, when coupled with mmx technology, is being targeted for the oral treatment of traveler's diarrhea (td) and clostridium difficile-associated disease (cdad). rifamycin sv was tested for activity against 911 td-associated enteropathogens and 30 c. difficile isolates collected from several global surveillance studies. rifamycin sv demonstrated similar antimicrobial activity levels against the enterobacteriaceae, with mic₅₀ v ... | 2010 | 21149623 |
| prevalence and antimicrobial resistance profile of campylobacter spp. isolated from conventional and antimicrobial-free swine production systems from different u.s. regions. | we conducted a study to compare the prevalence and antimicrobial resistance profile of campylobacter isolated from 34 farm-slaughter pair cohorts of pigs raised in conventional and antimicrobial-free (abf) production systems. isolates originated from four different states of two geographic regions (region 1--ohio and michigan; region 2--wisconsin and iowa). a total of 838 fecal and 1173 carcass samples were examined. campylobacter isolates were speciated using multiplex polymerase chain reaction ... | 2010 | 21133777 |
| prevalence and risk factors associated with campylobacter infections in broiler flocks in shiraz, southern iran. | campylobacter species are among the most common bacterial causes of human gastroenteritis in many countries, and poultry meat is considered as a major source of human campylobacteriosis. the present study was conducted to determine the prevalence of infection by campylobacter jejuni and campylobacter coli in broiler flocks in shiraz and to investigate the possible risk factors for the campylobacter infections in this area. for detection of campylobacter, multiplex polymerase chain reaction (mpcr ... | 2010 | 21131089 |
| typing of campylobacter jejuni and campylobacter coli isolated from live broilers and retail broiler meat by flaa-rflp, mlst, pfge and rep-pcr. | we analyzed 100 campylobacter spp. isolates (c. jejuni and c. coli) from grenada, puerto rico and alabama, which were collected from live broilers or retail broiler meat. we analyzed these isolates with four molecular typing methods: restriction fragment length polymorphism of the flaa gene (flaa-rflp), multilocus sequence typing (mlst), pulsed-field gel electrophoresis (pfge), and automated repetitive extragenic palindromic polymerase chain reaction (rep-pcr) using the diversilab system. all me ... | 2010 | 21130125 |
| interbacterial macromolecular transfer by the campylobacter fetus subsp. venerealis type iv secretion system. | we report here the first demonstration of intra- and interspecies conjugative plasmid dna transfer for campylobacter fetus. gene regions carried by a campylobacter coli plasmid were identified that are sufficient for conjugative mobilization to escherichia coli and c. fetus recipients. a broader functional range is predicted. efficient dna transfer involves the virb9 and vird4 genes of the type iv bacterial secretion system encoded by a pathogenicity island of c. fetus subsp. venerealis. complem ... | 2010 | 21115658 |
| tir domain-containing adaptor sarm is a late addition to the ongoing microbe-host dialog. | toll/interleukin-1 receptor (tir) domain-containing proteins play important roles in defense against pathogens in both animals and plants, connecting the immunity signaling pathways via a chain of specific protein-protein interactions. among them is sarm, the only tir domain-containing adaptor that can negatively regulate tlr signaling. by extensive phylogenetic analysis, we show here that sarm is closely related to bacterial proteins with tir domains, suggesting that this family has a different ... | 2010 | 21110998 |
| tir domain-containing adaptor sarm is a late addition to the ongoing microbe-host dialog. | toll/interleukin-1 receptor (tir) domain-containing proteins play important roles in defense against pathogens in both animals and plants, connecting the immunity signaling pathways via a chain of specific protein-protein interactions. among them is sarm, the only tir domain-containing adaptor that can negatively regulate tlr signaling. by extensive phylogenetic analysis, we show here that sarm is closely related to bacterial proteins with tir domains, suggesting that this family has a different ... | 2010 | 21110998 |
| enteric campylobacteria and rna viruses associated with healthy and diarrheic humans in the chinook health region of southwestern alberta, canada. | the presence of campylobacter species and enteric rna viruses in stools from diarrheic (n = 442) and healthy (n = 58) humans living in southwestern alberta was examined (may to october 2005). a large number of diarrheic individuals who were culture negative for c. jejuni (n = 54) or c. coli (n = 19) were pcr positive for these taxa. overall detection rates for c. jejuni and c. coli in diarrheic stools were 29% and 5%, respectively. in contrast, 3% and 0% of stools from healthy humans were positi ... | 2010 | 21106791 |
| enteric campylobacteria and rna viruses associated with healthy and diarrheic humans in the chinook health region of southwestern alberta, canada. | the presence of campylobacter species and enteric rna viruses in stools from diarrheic (n = 442) and healthy (n = 58) humans living in southwestern alberta was examined (may to october 2005). a large number of diarrheic individuals who were culture negative for c. jejuni (n = 54) or c. coli (n = 19) were pcr positive for these taxa. overall detection rates for c. jejuni and c. coli in diarrheic stools were 29% and 5%, respectively. in contrast, 3% and 0% of stools from healthy humans were positi ... | 2010 | 21106791 |
| independent evolution of the core and accessory gene sets in the genus neisseria: insights gained from the genome of neisseria lactamica isolate 020-06. | the genus neisseria contains two important yet very different pathogens, n. meningitidis and n. gonorrhoeae, in addition to non-pathogenic species, of which n. lactamica is the best characterized. genomic comparisons of these three bacteria will provide insights into the mechanisms and evolution of pathogenesis in this group of organisms, which are applicable to understanding these processes more generally. | 2010 | 21092259 |
| phenotypic and genotypic heterogeneity of campylobacter coli within individual pigs at farm and slaughter in the us. | the aim of this study was to determine the phenotypic and genotypic diversity of multiple campylobacter isolates (n = 3 per sample) present within individual (heterogeneity) pig faecal and carcass samples at farm and slaughter, respectively. we isolated 1459 campylobacter coli (1110 on farm and 349 from slaughter) from 908 pigs and 757 carcasses and characterized them for their antimicrobial susceptibility profile to a panel of six antimicrobials using the agar dilution method. overall, we detec ... | 2010 | 21083823 |
| microbiological quality of blue mussels (mytilus edulis) in nunavik, quebec: a pilot study. | this pilot study was aimed at documenting the presence of fecal indicators and enteric pathogens in blue mussels (mytilus edulis) from 6 communities in nunavik, quebec. one to four 2 kg samples of mussels were collected at low tide in each community. samples were investigated by enumeration methods for the fecal indicators enterococci, escherichia coli, f-specific coliphages, clostridium perfringens, and by molecular identification for the pathogens norovirus, salmonella spp., campylobacter jeju ... | 2010 | 21076488 |
| prevalence, numbers and antimicrobial susceptibilities of salmonella serovars and campylobacter spp. in retail poultry in phnom penh, cambodia. | salmonella and campylobacter are common bacterial pathogens associated with human gastro-enteritis; and raw poultry is considered to be an important source of these bacteria. to evaluate whether the salmonella serovars and campylobacter spp. bacteria could be monitored for the purpose of microbial presence, enumeration and antimicrobial resistance in raw poultry, 152 poultry carcasses were randomly selected from 10 markets in retail outlets of phnom penh during march 2006 to february 2007. the m ... | 2010 | 21060246 |
| campylobacter genotypes from poultry transportation crates indicate a source of contamination and transmission. | crates used to transport live poultry can be contaminated with campylobacter, despite periodic sanitization, and are potential vectors for transmission between flocks. we investigated the microbial contamination of standard and silver ion containing crates in normal use and the genetic structure of associated campylobacter populations. | 2010 | 21059158 |
| campylobacter genotypes from poultry transportation crates indicate a source of contamination and transmission. | crates used to transport live poultry can be contaminated with campylobacter, despite periodic sanitization, and are potential vectors for transmission between flocks. we investigated the microbial contamination of standard and silver ion containing crates in normal use and the genetic structure of associated campylobacter populations. | 2010 | 21059158 |
| flagellin as an adjuvant: cellular mechanisms and potential. | flagellin is a potent activator of a broad range of cell types involved in innate and adaptive immunity. an increasing number of studies have demonstrated the effectiveness of flagellin as an adjuvant, as well as its ability to promote cytokine production by a range of innate cell types, trigger a generalized recruitment of t and b lymphocytes to secondary lymphoid sites, and activate tlr5(+)cd11c(+) cells and t lymphocytes in a manner that is distinct from cognate ag recognition. the plasticity ... | 2010 | 21048152 |
| detection of gyra mutation among clinical isolates of campylobacter jejuni isolated in egypt by mama-pcr. | campylobacter spp are the major cause of enteritis in humans and more than 90% of reported infections are caused by campylobacter jejuni. fluoroquinolones such as ciprofloxacin are the antibiotics of choice for treatment. an increase in the frequency of ciprofloxacin-resistant campylobacter has been reported globally due to a single base mutation (c-257 to t) in codon 86 of the quinolone resistance determining region (qrdr) of the gyra gene altering the amino acid sequence from threonine at posi ... | 2010 | 21045366 |
| molecular characterization and environmental mapping of campylobacter isolates in a subset of intensive poultry flocks in ireland. | irish breeder and intensive broiler flocks together with the corresponding poultry farm environment were sampled for the presence of campylobacter with the aim of identifying potential sources and transmission routes of poultry flock contamination. the genetic diversity of a subset of campylobacter isolates was examined by analysis of the flaa-short variable region (svr). additional discrimination for a further subset of these isolates was achieved using multilocus sequence typing (mlst) analysi ... | 2010 | 21039133 |
| temperature affects sole carbon utilization patterns of campylobacter coli 49941. | campylobacter spp. are small, asaccharolytic bacteria exhibiting unique nutritional and environmental requirements. campylobacter spp. exist as commensal organisms in some animal species, yet are estimated to be the most common causative agents of foodborne illness in humans. c. jejuni is most often associated with poultry, while c. coli are more frequently associated with swine. temperature has been suggested to trigger potential colonization or virulence factors in c. jejuni, and recent studie ... | 2010 | 20981547 |
| antimicrobial resistance of campylobacter isolates from retail meat in the united states between 2002 and 2007. | the emergence of antimicrobial resistance in campylobacter spp. has been a growing public health concern globally. the objectives of this study were to determine the prevalence, antimicrobial susceptibility, and genetic relatedness of campylobacter spp. recovered by the national antimicrobial resistance monitoring system (narms) retail meat program. retail meat samples (n = 24,566) from 10 u.s. states collected between 2002 and 2007, consisting of 6,138 chicken breast, 6,109 ground turkey, 6,171 ... | 2010 | 20971875 |
| prevalence and characterization of campylobacter spp. isolated from domestic and imported poultry meat in korea, 2004–2008. | campylobacteriosis in humans is primarily caused by handling or consuming contaminated poultry or their products. the aims of this study were to estimate the prevalence of campylobacter spp. in domestic and imported poultry meat in korea and to further characterize the obtained isolates. from 2004 to 2008, a total of 475 domestic and 867 imported raw poultry meat samples were examined for the presence of campylobacter spp. among 475 domestic poultry meat samples, campylobacter jejuni and campylo ... | 2010 | 20939742 |
| simultaneous screening of 24 target genes of foodborne pathogens in 35 foodborne outbreaks using multiplex real-time sybr green pcr analysis. | a set of 8 multiplex real-time sybr green pcr (sg-pcr) assays including 3 target primers and an internal amplification control (iac) primer was simultaneously evaluated in 3 h or less with regard to detection of 24 target genes of 23 foodborne pathogens in 7 stool specimens of foodborne outbreak using a 96-well reaction plate. this assay, combined with dna extraction (qiaamp dna stool mini kit), offered detection of greater than 10(3)-10(4) foodborne pathogens per g in stool specimens. the produ ... | 2010 | 20936159 |
| evolution and population structure of salmonella enterica serovar newport. | salmonellosis caused by salmonella enterica serovar newport is a major global public health concern, particularly because s. newport isolates that are resistant to multiple drugs (mdr), including third-generation cephalosporins (mdr-ampc phenotype), have been commonly isolated from food animals. we analyzed 384 s. newport isolates from various sources by a multilocus sequence typing (mlst) scheme to study the evolution and population structure of the serovar. these were compared to the populatio ... | 2010 | 20935094 |
| direction of neutrophil movements by campylobacter-infected intestinal epithelium. | campylobacter jejuni and campylobacter coli together represent the leading bacterial cause of human enteritis. however, the pathogenesis of this disease is poorly understood. infection results in the formation of crypt abscesses resulting from the migration of neutrophils across the intestinal epithelium and into the intestinal crypts. in this study, we model this process in vitro and show that campylobacter infection of epithelium results in a quantifiable increase in the directed movement of n ... | 2010 | 20934530 |
| solexaqa: at-a-glance quality assessment of illumina second-generation sequencing data. | illumina's second-generation sequencing platform is playing an increasingly prominent role in modern dna and rna sequencing efforts. however, rapid, simple, standardized and independent measures of run quality are currently lacking, as are tools to process sequences for use in downstream applications based on read-level quality data. | 2010 | 20875133 |
| ribosomal operon intergenic sequence region (isr) heterogeneity in campylobacter coli and campylobacter jejuni. | the intergenic sequence regions (isr) between the 16s and 23s genes of campylobacter jejuni and campylobacter coli are markedly different for each species. however, in the genomic sequence for camp. coli rm2228, two rrna operons have an isr that is characteristic of camp. coli, and the third operon is characteristic of camp. jejuni. the aim of this study was to determine the prevalence of isr heterogeneity in these organisms. | 2010 | 20849393 |
| differences in methylation at gatc sites in genomic dna of campylobacter coli from turkeys and swine. | a significant fraction (46/108, 43%) of swine isolates of campylobacter coli but none of 81 isolates of c. coli from turkeys had genomic dna that was resistant to digestion by mboi, suggesting methylation of adenines at gatc sites. no consistent association was noted between antimicrobial resistance and mboi resistance. seven swine-associated multilocus sequence typing-based sequence types (sts) were detected among multiple isolates with mboi-resistant dna. the data suggest host-associated dna m ... | 2010 | 20833787 |
| survival of campylobacter jejuni and campylobacter coli on retail broiler meat stored at -20, 4, or 12 degrees c and development of weibull models for survival. | survival of campylobacter jejuni and campylobacter coli isolated from broiler meat was investigated and modeled on retail breast meat. meat portions were inoculated with c. jejuni or c. coli at 6.4 to 6.8 log cfu/g followed by storage at -20 degrees c for 84 days or at 4 or 12 degrees c for 14 days. kinetic data within a species and temperature were fitted to the weibull model. when >or=70% of the residuals were in an acceptable prediction zone from -1 (fail-safe) to 0.5 (fail-dangerous) log uni ... | 2010 | 20819353 |
| prevalence, antibiograms, and transferable tet(o) plasmid of campylobacter jejuni and campylobacter coli isolated from raw chicken, pork, and human clinical cases in korea. | the antibiotic resistance patterns and prevalence of the transferable tet(o) plasmid were investigated in campylobacter jejuni and campylobacter coli isolates from raw chicken, pork, and humans with clinical campylobacteriosis. a total of 180 c. jejuni and c. coli isolates were identified, and the prevalence rates of c. jejuni and c. coli in raw chicken samples were 83% (83 of 100) and 73% (73 of 100), respectively. twelve percent (6 of 50) and 10% (5 of 50) of pork samples were contaminated wit ... | 2010 | 20819352 |
| prevalence of campylobacter jejuni, campylobacter coli and enteric helicobacter in domestic and free living birds in north-western italy. | in order to investigate the prevalence of some thermophilic campylobacter (c. jejuni and c. coli) and enteric helicobacter (h. pullorum and h. canadensis) in domestic and wild birds, a total of 278 bird caecal samples were analyzed over a 2 year period in north-western italy. samples were collected from poultry raised in intensive farming at the slaughterhouse (n=102, group a) and in small scale rural farms (n=60, group b) as well as from wild birds (n=116, group c). pcr amplifications were carr ... | 2010 | 20814861 |
| fragmentation of the large subunit ribosomal rna gene in oyster mitochondrial genomes. | discontinuous genes have been observed in bacteria, archaea, and eukaryotic nuclei, mitochondria and chloroplasts. gene discontinuity occurs in multiple forms: the two most frequent forms result from introns that are spliced out of the rna and the resulting exons are spliced together to form a single transcript, and fragmented gene transcripts that are not covalently attached post-transcriptionally. within the past few years, fragmented ribosomal rna (rrna) genes have been discovered in bilatera ... | 2010 | 20813041 |
| comparison of premier campy enzyme immunoassay (eia), prospect campylobacter eia, and immunocard stat! campy tests with culture for laboratory diagnosis of campylobacter enteric infections. | campylobacter enteritis is a food-borne or waterborne illness caused almost exclusively by campylobacter jejuni and, to a lesser extent, by campylobacter coli. these organisms produce indistinguishable clinical diseases and together represent the second most common cause of bacterial diarrhea in the united states and the leading cause of enteric infection throughout the world. the conventional approach to the laboratory diagnosis of campylobacter enteritis is based on the recovery of the organis ... | 2010 | 20810765 |
| the in vivo efficacy of two administration routes of a phage cocktail to reduce numbers of campylobacter coli and campylobacter jejuni in chickens. | poultry meat is one of the most important sources of human campylobacteriosis, an acute bacterial enteritis which is a major problem worldwide. campylobacter coli and campylobacter jejuni are the most common campylobacter species associated with this disease. these pathogens live in the intestinal tract of most avian species and under commercial conditions they spread rapidly to infect a high proportion of the flock, which makes their treatment and prevention very difficult. bacteriophages (phag ... | 2010 | 20809975 |
| abc transporters involved in export of cell surface glycoconjugates. | complex glycoconjugates play critical roles in the biology of microorganisms. despite the remarkable diversity in glycan structures and the bacteria that produce them, conserved themes are evident in the biosynthesis-export pathways. one of the primary pathways involves representatives of the atp-binding cassette (abc) transporter superfamily. these proteins are responsible for the export of a wide variety of cell surface oligo- and polysaccharides in both gram-positive and gram-negative bacteri ... | 2010 | 20805402 |
| the glycerophospho metabolome and its influence on amino acid homeostasis revealed by brain metabolomics of gde1(-/-) mice. | gde1 is a mammalian glycerophosphodiesterase (gde) implicated by in vitro studies in the regulation of glycerophophoinositol (gropins) and possibly other glycerophospho (grop) metabolites. here, we show using untargeted metabolomics that gropins is profoundly (>20-fold) elevated in brain tissue from gde1(-/-) mice. furthermore, two additional grop metabolites not previously identified in eukaryotic cells, glycerophosphoserine (gropser) and glycerophosphoglycerate (gropgate), were also highly ele ... | 2010 | 20797612 |
| obstacles of multiplex real-time pcr for bacterial 16s rdna: primer specifity and dna decontamination of taq polymerase. | background: the detection of a broad range of bacteria by pcr is applied for the screening of blood and blood products with special attention to platelet concentrates. for practical use it is desirable that detection systems include gram-positive, gram-negative and non-gram-stainable bacteria. it is quite challenging to achieve high sensitivity along with a clear negative control with pcr reagents, because especially taq polymerase is contaminated with traces of bacterial dna. methods: bacterial ... | 2010 | 20737013 |
| characterization of erythromycin-resistant porcine isolates of campylobacter coli. | erythromycin-resistant campylobacter organisms were isolated from swine, and their resistance to the antibiotic was characterized. one hundred fourteen campylobacter organisms were isolated from 572 swine intestinal samples. all isolates were identified as campylobacter coli by sequence analysis of 16s rrna gene and polymerase chain reactions with primers specific to hippuricase gene in campylobacter jejuni and aspartokinase gene in c. coli. minimal inhibitory concentrations (mics) of erythromyc ... | 2010 | 20735175 |
| occurrences of thermophilic campylobacter in pigs slaughtered at morogoro slaughter slabs, tanzania. | occurrences of thermophlic campylobacter in pigs and pig carcasses was investigated in a cross-sectional study that was carried out in three selected slaughter slabs in morogoro municipality, tanzania. before sampling, the slab hygiene, slaughter, carcass dressing, and meat handling was assessed. fecal samples were collected from 66 slaughter pigs at the kill floor. after slaughter, a 100-cm(2) area on medial surface of the thigh muscles of dressed carcasses was sampled using sterile cotton swab ... | 2011 | 20730490 |
| genotype and antibiotic resistance analyses of campylobacter isolates from ceca and carcasses of slaughtered broiler flocks. | to obtain genetic information about campylobacter jejuni and campylobacter coli from broilers and carcasses at slaughterhouses, we analyzed and compared 340 isolates that were collected in 2008 from the cecum right after slaughter or from the neck skin after processing. we performed rpob sequence-based identification, multilocus sequence typing (mlst), and flab sequence-based typing; we additionally analyzed mutations within the 23s rrna and gyra genes that confer resistance to macrolide and qui ... | 2010 | 20709846 |
| evolutionary dynamics of complete campylobacter pan-genomes and the bacterial species concept. | defining bacterial species and understanding the relative cohesiveness of different components of their genomes remains a fundamental problem in microbiology. bacterial species tend to be comprised of both a set of core and dispensable genes, with the sum of these two components forming the species pan-genome. the role of the core and dispensable genes in defining bacterial species and the question of whether pan-genomes are finite or infinite remain unclear. here we demonstrate, through the ana ... | 2010 | 20688752 |
| antimicrobial resistance in campylobacter coli selected by tylosin treatment at a pig farm. | limited knowledge is available regarding the dynamics of macrolide resistance under farm conditions with natural campylobacter populations. we examined the dynamics of antimicrobial resistance in campylobacter coli at a large pig farm. faeces were sampled from untreated sows and piglets (n=57), weaned pigs treated with tylosin (n=68) and pigs of the same group 3-5 weeks after withdrawal of tylosin (n=15). additionally, 48 weaned pigs were sampled after tylosin had not been administered for 7 mon ... | 2010 | 20684867 |
| accuracy of the api campy system, the vitek 2 neisseria-haemophilus (nh) card and the matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (maldi-tof ms) for the identification of campylobacter and related organisms. | abstract biochemical identification of campylobacter and related organisms is not always specific and may lead to diagnostic errors. the api campy, the vitek 2 system and matrix-assisted desorption/ionisation time-of-flight mass spectrometry (maldi-tof ms) are commercially available methods that are routinely used for the identification of these microorganisms. in the present study, we used 224 clinical isolates and 10 reference strains previously identified by multiple pcr assays, whole-cell pr ... | 2010 | 20673261 |
| implications of faecal indicator bacteria for the microbiological assessment of roof-harvested rainwater quality in southeast queensland, australia. | the study aimed to evaluate the suitability of escherichia coli, enterococci, and clostridium perfringens for assessing the microbiological quality of roof-harvested rainwater and assessing whether the concentrations of these faecal indicators can be used to predict the presence or absence of specific zoonotic bacterial or protozoan pathogens. from a total of 100 samples tested, 58%, 83%, and 46% of samples were found to be positive for, respectively, e. coli, enterococci, and clostridium perfri ... | 2010 | 20657617 |
| study of the molecular mechanisms involved in high-level macrolide resistance of spanish campylobacter jejuni and campylobacter coli strains. | to investigate the molecular mechanisms involved in the high-level erythromycin resistance of clinical spanish campylobacter jejuni and campylobacter coli strains. | 2010 | 20647243 |
| survival of campylobacter jejuni under conditions of atmospheric oxygen tension with the support of pseudomonas spp. | campylobacter jejuni is a major food-borne pathogen. despite causing enteritis in humans, it is a well-adapted intestinal microorganism in animals, hardly ever generating disease symptoms. nevertheless, as a true microaerophilic microorganism it is still puzzling how campylobacter cells can survive on chicken meat, the main source of human infection. in this study, we demonstrate that c. jejuni is able to withstand conditions of atmospheric oxygen tension when cocultured with pseudomonas species ... | 2010 | 20639377 |
| isolation and characterization of campylobacter spp. from antarctic fur seals (arctocephalus gazella) at deception island, antarctica. | the presence of campylobacter spp. was investigated in 41 antarctic fur seals (arctocephalus gazella) and 9 weddell seals (leptonychotes weddellii) at deception island, antarctica. infections were encountered in six antarctic fur seals. the isolates, the first reported from marine mammals in the antarctic region, were identified as campylobacter insulaenigrae and campylobacter lari. | 2010 | 20639356 |
| simple and rapid detection of campylobacter spp. in naturally contaminated chicken-meat samples by combination of a two-step enrichment method with an immunochromatographic assay. | a simple and rapid method to detect campylobacter spp. in chicken-meat samples was established. this method consisted of a combination of a two-step enrichment method with a commercially available immunochromatographic assay, named nh immunochromato campylobacter (nh ic campy, nippon meat packers, ibaraki, japan), which is able to detect campylobacter antigen in an enrichment culture within 15 min. the enrichment method did not require much blood or a particular system of generating a microaerob ... | 2010 | 20621373 |
| carry-over of thermophilic campylobacter spp. between sequential and adjacent poultry flocks. | nineteen flocks of four poultry species were monitored at a veterinary field station to investigate the distribution and spread of campylobacter genotypes between sequential and adjacent flocks. caecal and liver samples were obtained at frequent intervals from birds of all flocks and examined for campylobacter. amplified fragment length polymorphism (aflp) analysis was performed to genotype campylobacter isolates. of the 1643 caecal and liver samples investigated, 452 (27.5%) caecal samples and ... | 2011 | 20619560 |
| prevalence and antimicrobial resistance in campylobacter spp. isolated from retail chicken in two health units in ontario. | campylobacter is an important enteric pathogen of humans and can cause diarrhea, fever, and abdominal pain. campylobacter infections have frequently been associated with the handling and consumption of raw and undercooked poultry. antimicrobial resistance among campylobacter strains is of concern in the treatment of campylobacteriosis in vulnerable populations. a 2-year multidisciplinary study was conducted in the perth and wellington-dufferin-guelph public health units in ontario, canada, to in ... | 2010 | 20615345 |
| campylobacter spp among children with acute diarrhea attending mulago hospital in kampala--uganda. | campylobacter infections occur worldwide. a recent study in kampala, uganda, found that 87% of broiler chickens had campylobacter jejuni; these are potential source of human infection. isolation rate in developing countries is between 5-35%. this study aimed at finding prevalence of children with campylobacter infection among children with acute diarrhea attending mulago hospital. | 2009 | 20589152 |
| identification and characterization of a new ferric enterobactin receptor, cfrb, in campylobacter. | the ferric enterobactin (feent) receptor cfra is present in the majority of campylobacter jejuni isolates and is responsible for high-affinity iron acquisition. our recent work and that of others strongly suggested the existence of another feent uptake system in campylobacter. here we have identified and characterized a new feent receptor (designated cfrb) using both in vitro and in vivo systems. cfrb, a homolog of c. jejuni nctc 11168 cj0444, shares approximately 34% of amino acid identity with ... | 2010 | 20585060 |
| characterization of n-linked protein glycosylation in helicobacter pullorum. | the first bacterial n-linked glycosylation system was discovered in campylobacter jejuni, and the key enzyme involved in the coupling of glycan to asparagine residues within the acceptor sequon of the glycoprotein is the oligosaccharyltransferase pglb. emerging genome sequence data have revealed that pglb orthologues are present in a subset of species from the deltaproteobacteria and epsilonproteobacteria, including three helicobacter species: h. pullorum, h. canadensis, and h. winghamensis. in ... | 2010 | 20581208 |
| screening for several potential pathogens in feral pigeons (columba livia) in madrid. | pathogens with the zoonotic potential to infect humans, such as campylobacter jejuni, campylobacter coli and chlamydophila psittaci, can be found in feral pigeons (columba livia). given the high density of these birds in the public parks and gardens of most cities, they may pose a direct threat to public health. | 2010 | 20569487 |
| performance of the oxoid m.i.c.evaluator strips compared with the etest assay and bsac agar dilution. | the oxoid m.i.c.evaluator (m.i.c.e; thermo fisher scientific) comprises an antibiotic gradient on a plastic support. we compared its performance with etest--a similar product--using bsac agar dilution as a reference. | 2010 | 20562429 |
| campylobacter jejuni and campylobacter coli in children from communities in northeastern brazil: molecular detection and relation to nutritional status. | this study determined the prevalence of campylobacter jejuni/coli and its relation with nutritional status in children from northeastern brazil. this was a case-control study design. stool samples were evaluated for hipo (c. jejuni), ask (c. coli), and cdtabc (c. jejuni's cytolethal distending toxin) genes. the nutritional status from these children was assessed by anthropometric measures and z-scores. c. jejuni and c. coli were detected in 9.6% (8/83) and 6.0% (5/83) in the diarrhea group and i ... | 2010 | 20542202 |
| fluoroquinolone resistance in campylobacter. | campylobacter is a commensal in poultry, and therefore, poultry and poultry products are major sources of campylobacter infections in humans. fluoroquinolones inhibit the growth of campylobacter and other microorganisms by binding to bacterial dna gyrase and dna topoisomerase iv. these enzymes are associated with bacterial transcription, replication, and chromosome condensation and segregation. selection pressure in the presence of fluoroquinolones rapidly leads to resistance in campylobacter, d ... | 2010 | 20537275 |
| comparison of real-time pcr assays for detection, quantification, and differentiation of campylobacter jejuni and campylobacter coli in broiler neck skin samples. | we tested the use of multiplex real-time pcr for detection and quantification of campylobacter jejuni and campylobacter coli on broiler carcass neck skin samples collected during 2008 from slaughterhouses in switzerland. results from an established taqman assay based on two different targets (hipo and ceue for c. jejuni and c. coli, respectively) were corroborated with data from a newly developed assay based on a single-nucleotide polymorphism in the fusa gene, which allows differentiation betwe ... | 2010 | 20537260 |
| molecular typing of campylobacter jejuni and c. coli from chickens and patients with gastritis or guillain-barré syndrome based on multilocus sequence types and pulsed-field gel electrophoresis patterns. | campylobacter jejuni has recently been noted as the most common cause of bacterial foodborne diseases in japan. in the present study, we determined st types of c. jejuni and campylobacter coli isolated from chickens and patients with enteritis or gbs in japan and thailand. c. jejuni from chickens, enteritis, and gbs exhibited divergent st types and included several novel types in addition to worldwide common types. c. coli from enteritis was also divergent. novel st types may represent unidentif ... | 2010 | 20536735 |
| host association of campylobacter genotypes transcends geographic variation. | genetic attribution of bacterial genotypes has become a major tool in the investigation of the epidemiology of campylobacteriosis and has implicated retail chicken meat as the major source of human infection in several countries. to investigate the robustness of this approach to the provenance of the reference data sets used, a collection of 742 campylobacter jejuni and 261 campylobacter coli isolates obtained from united kingdom-sourced chicken meat was established and typed by multilocus seque ... | 2010 | 20525862 |
| three-hour molecular detection of campylobacter, salmonella, yersinia, and shigella species in feces with accuracy as high as that of culture. | campylobacter jejuni and salmonella, shigella, and yersinia species (along with shiga toxin-producing escherichia coli) are the most common causes of acute bacterial diarrheal disease in the united states. current detection techniques are time-consuming, limiting usefulness for patient care. we developed and validated a panel of rapid pcr assays for the detection and identification of c. jejuni, c. coli, salmonella, and yersinia species and shigella and enteroinvasive e. coli in stool samples. a ... | 2010 | 20519461 |
| identification of campylobacter jejuni genes involved in its interaction with epithelial cells. | campylobacter jejuni is the leading cause of infectious gastroenteritis in industrialized nations. its ability to enter and survive within nonphagocytic cells is thought to be very important for pathogenesis. however, little is known about the c. jejuni determinants that mediate these processes. through an extensive transposon mutagenesis screen, we have identified several loci that are required for c. jejuni efficient entry and survival within epithelial cells. among these loci, insertional mut ... | 2010 | 20515930 |
| investigation of water washes suitable for very small meat plants to reduce pathogens on beef surfaces. | water washing with a handheld hose was performed on beef surfaces to ascertain the most effective combination of methods needed to remove potentially harmful microorganisms. for these experiments, beef brisket surfaces were experimentally inoculated with a fecal slurry containing escherichia coli o157:h7, salmonella typhimurium, campylobacter coli, and campylobacter jejuni. in a pilot study, surfaces were washed with cold water (15 degrees c) at various water pressures, spray distances, applicat ... | 2010 | 20501042 |
| prevalence and antimicrobial resistance of campylobacter jejuni and campylobacter coli isolated from cattle between 2002 and 2006 in france. | feces from 2,255 cattle (calves, young beef cattle, and culled cows) were collected at slaughter from nine departments across france. campylobacter was recovered from 16.5% of the 2,255 samples (c. jejuni from 12.8% and c. coli from 3.7%), predominantly from calves. antimicrobial resistance to six antibiotics of medical and/or veterinary interest was tested with the e-test. resistance to tetracycline was found in most isolates (52.8% of c. jejuni isolates and 88.1% of c. coli isolates) in contra ... | 2010 | 20501032 |