Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| presence of oral bacterial species in primary endodontic infections of primary teeth. | knowledge of the microbial composition of deciduous endodontic infections is limited. this study aimed to evaluate the presence of the 10 oral bacterial species in samples from primary tooth root canals by using microarray technology and to determine the association of these organisms with clinical conditions. | 2013 | 24683780 |
| oral cavity anaerobic pathogens in biofilm formation on voice prostheses. | a polymerase chain reaction (pcr)-based method has been used to identify oral anaerobic pathogens in biofilms on voice prostheses. the purpose of the present study was to determine the location of those pathogens inside the biofilms. | 2015 | 24677258 |
| bacteriological analysis of necrotic pulp and fistulae in primary teeth. | primary teeth work as guides for the eruption of permanent dentition, contribute for the development of the jaws, chewing process, preparing food for digestion, and nutrient assimilation. treatment of pulp necrosis in primary teeth is complex due to anatomical and physiological characteristics and high number of bacterial species present in endodontic infections. the bacterial presence alone or in association in necrotic pulp and fistula samples from primary teeth of boys and girls was evaluated ... | 2014 | 24676582 |
| discovery of a super-strong promoter enables efficient production of heterologous proteins in cyanobacteria. | cyanobacteria are oxygenic photosynthetic prokaryotes that play important roles in the global carbon cycle. recently, engineered cyanobacteria capable of producing various small molecules from co2 have been developed. however, cyanobacteria are seldom considered as factories for producing proteins, mainly because of the lack of efficient strong promoters. here, we report the discovery and verification of a super-strong promoter p(cpc560), which contains two predicted promoters and 14 predicted t ... | 2014 | 24675756 |
| massive parallel sequencing provides new perspectives on bacterial brain abscesses. | rapid development within the field of massive parallel sequencing (mps) is about to bring this technology within reach for diagnostic microbiology laboratories. we wanted to explore its potential for improving diagnosis and understanding of polymicrobial infections, using bacterial brain abscesses as an example. we conducted a prospective nationwide study on bacterial brain abscesses. fifty-two surgical samples were included over a 2-year period. the samples were categorized as either spontaneou ... | 2014 | 24671797 |
| phylogenetic and functional gene structure shifts of the oral microbiomes in periodontitis patients. | determining the composition and function of subgingival dental plaque is crucial to understanding human periodontal health and disease, but it is challenging because of the complexity of the interactions between human microbiomes and human body. here, we examined the phylogenetic and functional gene differences between periodontal and healthy individuals using miseq sequencing of 16s rrna gene amplicons and a specific functional gene array (a combination of geochip 4.0 for biogeochemical process ... | 2014 | 24671083 |
| biodegradation pattern and tissue integration of native and cross-linked porcine collagen soft tissue augmentation matrices - an experimental study in the rat. | within the last decades, collagen types i and iii have been established as a sufficient biomaterial for gbr and gtr procedures. they might also be an adequate matrix for soft tissue augmentations. however, collagen materials differ significantly regarding resorption time, biodegradation pattern and the invasion of inflammatory cells.the aim of the present study was to compare the biodegradation and tissue integration of native, differently processed and cross-linked collagen scaffolds in rats. | 2014 | 24670219 |
| microbiological assessment of the implant-abutment interface in different connections: cross-sectional study after 5 years of functional loading. | to evaluate the bacterial microflora present inside the implant connection and in the peri-implant sulcus fluid of healthy implants, and to analyze the relationships between these harboring sites for four different implant systems after at least 5 years of functional loading. | 2015 | 24665919 |
| atherosclerosis and alzheimer--diseases with a common cause? inflammation, oxysterols, vasculature. | aging is accompanied by increasing vulnerability to pathologies such as atherosclerosis (ath) and alzheimer disease (ad). are these different pathologies, or different presentations with a similar underlying pathoetiology? | 2014 | 24656052 |
| predictive modeling of gingivitis severity and susceptibility via oral microbiota. | predictive modeling of human disease based on the microbiota holds great potential yet remains challenging. here, 50 adults underwent controlled transitions from naturally occurring gingivitis, to healthy gingivae (baseline), and to experimental gingivitis (eg). in diseased plaque microbiota, 27 bacterial genera changed in relative abundance and functional genes including 33 flagellar biosynthesis-related groups were enriched. plaque microbiota structure exhibited a continuous gradient along the ... | 2014 | 24646694 |
| gingival tissue transcriptomes identify distinct periodontitis phenotypes. | the currently recognized principal forms of periodontitis-chronic and aggressive-lack an unequivocal, pathobiology-based foundation. we explored whether gingival tissue transcriptomes can serve as the basis for an alternative classification of periodontitis. we used cross-sectional whole-genome gene expression data from 241 gingival tissue biopsies obtained from sites with periodontal pathology in 120 systemically healthy nonsmokers with periodontitis, with available data on clinical periodontal ... | 2014 | 24646639 |
| the role of oral pathobionts in dysbiosis during periodontitis development. | an emerging concept is the tight relationship between dysbiosis (microbiota imbalance) and disease. the increase in knowledge about alterations in microbial communities that reside within the host has made a strong impact not only on dental science, but also on immunology and microbiology as well as on our understanding of several diseases. periodontitis is a well-characterized human disease associated with dysbiosis, characterized by the accumulation of multiple bacteria that play individual an ... | 2014 | 24646638 |
| characterization of aid1, a novel gene involved in fusobacterium nucleatum interspecies interactions. | the oral opportunistic pathogen fusobacterium nucleatum is known to interact with a large number of different bacterial species residing in the oral cavity. it adheres to a variety of gram-positive bacteria, including oral streptococci via the arginine-inhibitable adhesin radd. in this study, we describe a novel protein encoded by the predicted open reading frame fn1253 that appears to play a role in interspecies interactions of f. nucleatum, particularly with oral streptococci and related gram- ... | 2014 | 24643713 |
| application of metagenomics in understanding oral health and disease. | oral diseases including periodontal disease and caries are some of the most prevalent infectious diseases in humans. different microbial species cohabitate and form a polymicrobial biofilm called dental plaque in the oral cavity. metagenomics using next generation sequencing technologies has produced bacterial profiles and genomic profiles to study the relationships between microbial diversity, genetic variation, and oral diseases. several oral metagenomic studies have examined the oral microbio ... | 2014 | 24642489 |
| bis-enoxacin blocks rat alveolar bone resorption from experimental periodontitis. | periodontal diseases are multifactorial, caused by polymicrobial subgingival pathogens, including porphyromonas gingivalis, treponema denticola, and tannerella forsythia. chronic periodontal infection results in inflammation, destruction of connective tissues, periodontal ligament, and alveolar bone resorption, and ultimately tooth loss. enoxacin and a bisphosphonate derivative of enoxacin (bis-enoxacin) inhibit osteoclast formation and bone resorption and also contain antibiotic properties. our ... | 2014 | 24638087 |
| functional diversity of the microbial community in healthy subjects and periodontitis patients based on sole carbon source utilization. | chronic periodontitis is one of the most common forms of biofilm-induced diseases. most of the recent studies were focus on the dental plaque microbial diversity and microbiomes. however, analyzing bacterial diversity at the taxonomic level alone limits deeper comprehension of the ecological relevance of the community. in this study, we compared the metabolic functional diversity of the microbial community in healthy subjects and periodontitis patients in a creative way--to assess the sole carbo ... | 2014 | 24632674 |
| targeting cystalysin, a virulence factor of treponema denticola-supported periodontitis. | cystalysin from treponema denticola is a pyridoxal 5'-phosphate dependent lyase that catalyzes the formation of pyruvate, ammonia, and sulfide from cysteine. it is a virulence factor in adult periodontitis because its reaction contributes to hemolysis, which sustains the pathogen. therefore, it was proposed as a potential antimicrobial target. to identify specific inhibitors by structure-based in silico methods, we first validated the crystal structure of cystalysin as a reliable starting point ... | 2014 | 24616267 |
| peri-implant response and microflora in organ transplant patients 1 year after prosthetic loading: a prospective controlled study. | a recent study conducted in humans demonstrated for the first time that bone loss in the immediate period after implant insertion before loading did not significantly differ in organ transplant recipients with respect to normal subjects. | 2015 | 24612063 |
| potential role of bacterial infection in autoimmune diseases: a new aspect of molecular mimicry. | molecular mimicry is an attractive mechanism for triggering autoimmunity. in this review, we explore the potential role of evolutionary conserved bacterial proteins in the production of autoantibodies with focus on granulomatosis with polyangiitis (gpa) and rheumatoid arthritis (ra). seven autoantigens characterized in gpa and ra were blasted against a bacterial protein database. of the seven autoantigens, proteinase 3, type ii collagen, binding immunoglobulin protein, glucose-6-phosphate isomer ... | 2014 | 24605075 |
| porphyromonas gingivalis and treponema denticola exhibit metabolic symbioses. | porphyromonas gingivalis and treponema denticola are strongly associated with chronic periodontitis. these bacteria have been co-localized in subgingival plaque and demonstrated to exhibit symbiosis in growth in vitro and synergistic virulence upon co-infection in animal models of disease. here we show that during continuous co-culture a p. gingivalis:t. denticola cell ratio of 6∶1 was maintained with a respective increase of 54% and 30% in cell numbers when compared with mono-culture. co-cultur ... | 2014 | 24603978 |
| familial oral microbial imbalance and dental caries occurrence in their children. | develop a familial liability index for oral microbial status that reflects an imbalance of oral domains based on the presence of risk indicators in saliva, inter-proximal plaque, tongue, and throat. | 2013 | 24600078 |
| community-wide transcriptome of the oral microbiome in subjects with and without periodontitis. | despite increasing knowledge on phylogenetic composition of the human microbiome, our understanding of the in situ activities of the organisms in the community and their interactions with each other and with the environment remains limited. characterizing gene expression profiles of the human microbiome is essential for linking the role of different members of the bacterial communities in health and disease. the oral microbiome is one of the most complex microbial communities in the human body a ... | 2014 | 24599074 |
| xylitol, an anticaries agent, exhibits potent inhibition of inflammatory responses in human thp-1-derived macrophages infected with porphyromonas gingivalis. | xylitol is a well-known anticaries agent and has been used for the prevention and treatment of dental caries. in this study, the anti-inflammatory effects of xylitol are evaluated for possible use in the prevention and treatment of periodontal infections. | 2014 | 24592909 |
| both 25-hydroxyvitamin-d3 and 1,25-dihydroxyvitamin-d3 reduces inflammatory response in human periodontal ligament cells. | periodontitis is an inflammatory disease leading to the destruction of periodontal tissue. vitamin d3 is an important hormone involved in the preservation of serum calcium and phosphate levels, regulation of bone metabolism and inflammatory response. recent studies suggest that vitamin d3 metabolism might play a role in the progression of periodontitis. the aim of the present study was to examine the effects of 25(oh)d3, which is stable form of vitamin d3 in blood, and biologically active form 1 ... | 2014 | 24587317 |
| major membrane protein tde2508 regulates adhesive potency in treponema denticola. | the cultivation and genetic manipulation of treponema denticola, a gram-negative oral spirochaeta associated with periodontal diseases, is still challenging. in this study, we formulated a simple medium based on a commercially available one, and established a transformation method with high efficiency. we then analyzed proteins in a membrane fraction in t. denticola and identified 16 major membrane-associated proteins, and characterized one of them, tde2508, whose biological function was not yet ... | 2014 | 24586498 |
| crispr-cas systems for editing, regulating and targeting genomes. | targeted genome editing using engineered nucleases has rapidly gone from being a niche technology to a mainstream method used by many biological researchers. this widespread adoption has been largely fueled by the emergence of the clustered, regularly interspaced, short palindromic repeat (crispr) technology, an important new approach for generating rna-guided nucleases, such as cas9, with customizable specificities. genome editing mediated by these nucleases has been used to rapidly, easily and ... | 2014 | 24584096 |
| computational prediction of the human-microbial oral interactome. | the oral cavity is a complex ecosystem where human chemical compounds coexist with a particular microbiota. however, shifts in the normal composition of this microbiota may result in the onset of oral ailments, such as periodontitis and dental caries. in addition, it is known that the microbial colonization of the oral cavity is mediated by protein-protein interactions (ppis) between the host and microorganisms. nevertheless, this kind of ppis is still largely undisclosed. to elucidate these int ... | 2014 | 24576332 |
| invasion of oral and aortic tissues by oral spirochete treponema denticola in apoe(-/-) mice causally links periodontal disease and atherosclerosis. | treponema denticola is a predominantly subgingival oral spirochete closely associated with periodontal disease and has been detected in atherosclerosis. this study was designed to evaluate causative links between periodontal disease induced by chronic oral t. denticola infection and atherosclerosis in hyperlipidemic apoe(-/-) mice. apoe(-/-) mice (n = 24) were orally infected with t. denticola atcc 35404 and were euthanized after 12 and 24 weeks. t. denticola genomic dna was detected in oral pla ... | 2014 | 24566627 |
| pathogens and host immunity in the ancient human oral cavity. | calcified dental plaque (dental calculus) preserves for millennia and entraps biomolecules from all domains of life and viruses. we report the first, to our knowledge, high-resolution taxonomic and protein functional characterization of the ancient oral microbiome and demonstrate that the oral cavity has long served as a reservoir for bacteria implicated in both local and systemic disease. we characterize (i) the ancient oral microbiome in a diseased state, (ii) 40 opportunistic pathogens, (iii) ... | 2014 | 24562188 |
| crispr-based technologies: prokaryotic defense weapons repurposed. | to combat potentially deadly viral infections, prokaryotic microbes enlist small rna-based adaptive immune systems (crispr-cas systems) that protect through sequence-specific recognition and targeted destruction of viral nucleic acids (either dna or rna depending on the system). here, we summarize rapid progress made in redirecting the nuclease activities of these microbial immune systems to bind and cleave dna or rna targets of choice, by reprogramming the small guide rnas of the various crispr ... | 2014 | 24555991 |
| bacteremia following scaling and root planing: a clinico-microbiological study. | bacteremia frequently occurs after treatment procedures such as extractions, scaling, root planing, periodontal surgery. there is currently significant interest in the possibility that bacteremia with oral bacteria may play role in pathogenesis of atherosclerosis. there are well-conducted studies that have determined the frequency of passage of periodontal microorganisms to the bloodstream after periodontal treatment. there is scarce information related to the incidence of periodontopathic micro ... | 2013 | 24554880 |
| prevelance of periodontopathogenic bacteria in subgingival biofilm and atherosclerotic plaques of patients undergoing coronary revascularization surgery. | the objective of the present study was to detect the presence of specific periodontopathogenic bacteria in the coronary plaque of patients with coronary artery disease and to find out the significant association between the periodontal status and the presence of pathogenic bacteria in the coronary plaque. | 2013 | 24554879 |
| periodontitis and buerger's disease: recent advances. | buerger's disease (bd) is a relatively rare thrombotic, occlusive and non-atherosclerotic clinical syndrome of unknown etiology. in recent years, numerous epidemiological studies confirmed the strong association between chronic anaerobic periodontal infection and development of cardiovascular diseases, including bd. therefore, the aim of this study is to clarify association between periodontal pathogens and buerger's disease. confirmation of presence and identification of periopathogens in patie ... | 2013 | 24554799 |
| application of a diode laser in the reduction of targeted periodontal pathogens. | periodontal disease belongs to a group of diseases with more than one cause, it is a disease of a multifactorial etiology. although bacteria are the main cause of the disease, immunoinflammatory reaction of the host is responsible for the majority of destructive changes in periodontal tissue. the main issue in the evaluation of the success of periodontal therapy is the pluralism of the bacteria and their dynamic changes during the duration, on the one hand, and the possible inaccuracy of classic ... | 2013 | 24554796 |
| subgingival plaque sampling after combined mechanical and antibiotic nonsurgical periodontal therapy. | this study aimed to make a comparison of two sampling strategies of subgingival plaque after combined mechanical-antibiotic periodontal therapy. | 2015 | 24554003 |
| single cell genomics of uncultured, health-associated tannerella bu063 (oral taxon 286) and comparison to the closely related pathogen tannerella forsythia. | the uncultivated bacterium tannerella bu063 (oral taxon 286) is the closest relative to the periodontal pathogen tannerella forsythia, but is not disease-associated itself. using a single cell genomics approach, we isolated 12 individual bu063 cells by flow cytometry, and we amplified and sequenced their genomes. comparative analyses of the assembled genomic scaffolds and their gene contents allowed us to study the diversity of this taxon within the oral community of a single human donor that pr ... | 2014 | 24551246 |
| subgingival periodontal pathogens associated with chronic periodontitis in yemenis. | subgingival microbial profile associated with periodontitis has been reported to significantly differ by geographical location. the purpose of this study was to assess the association between a panel of putative periodontal bacterial pathogens and chronic periodontitis among yemenis. | 2014 | 24548674 |
| association between hypertension and periodontitis: possible mechanisms. | this review is to examine the current literatures on the relationship between periodontitis and hypertension as well as to explore the possible biological pathways underlying the linkage between these health conditions. hypertension is one of the major risk factors for cardiovascular diseases. oxidative stress and endothelial dysfunction are among the critical components in the development of hypertension. inflammation has received much attention recently and may contribute to a pivotal role in ... | 2014 | 24526921 |
| dental calculus is associated with death from heart infarction. | we studied whether the amount of dental calculus is associated with death from heart infarction in the dental infection-atherosclerosis paradigm. | 2014 | 24511535 |
| effect of oral administration involving a probiotic strain of lactobacillus reuteri on pro-inflammatory cytokine response in patients with chronic periodontitis. | this study aimed at evaluation of pro-inflammatory cytokine response (tnf-α, il-1β and il-17) in patients with chronic periodontitis administered per os with a probiotic strain of lactobacillus reuteri. in the 38 adult patients with moderate chronic periodontitis, professional cleaning of teeth was performed. two weeks after performing the oral hygienization procedures, clinical examination permitted to distinguish a group of 24 patients (group 1) in whom treatment with probiotic tablets contain ... | 2014 | 24509697 |
| looking in the porphyromonas gingivalis cabinet of curiosities: the microbium, the host and cancer association. | the past decades of biomedical research have yielded massive evidence for the contribution of the microbiome in the development of a variety of chronic human diseases. there is emerging evidence that porphyromonas gingivalis, a well-adapted opportunistic pathogen of the oral mucosa and prominent constituent of oral biofilms, best known for its involvement in periodontitis, may be an important mediator in the development of a number of multifactorial and seemingly unrelated chronic diseases, such ... | 2014 | 24506890 |
| transcriptional responses of treponema denticola to other oral bacterial species. | the classic organization by socransky and coworkers categorized the oral bacteria of the subgingival plaque into different complexes. treponema denticola, porphyromonas gingivalis and tannerella forsythia are grouped into the red complex that is highly correlated with periodontal disease. socransky's work closely associates red with orange complex species such as fusobacterium nucleatum and prevotella intermedia but not with members of the other complexes. while the relationship between species ... | 2014 | 24505483 |
| chair-side quantitative oral-microflora screening for assessing familial correlation of periodontal status and caries prevalence. | our goal was to investigate the relationship between clinical status and the presence of carious or periodontal pathogens among parent-child familial pairs. clinical practices of risk assessment with consideration of familial pathogen interaction might reduce the need for therapy, improve patient outcomes, and ultimately reduce oral disease burden. | 2014 | 24498022 |
| the personal human oral microbiome obscures the effects of treatment on periodontal disease. | periodontitis is a progressive disease of the periodontium with a complex, polymicrobial etiology. recent next-generation sequencing (ngs) studies of the microbial diversity associated with periodontitis have revealed strong, community-level differences in bacterial assemblages associated with healthy or diseased periodontal sites. in this study, we used ngs approaches to characterize changes in periodontal pocket bacterial diversity after standard periodontal treatment. despite consistent chang ... | 2014 | 24489772 |
| periodontal disease bacteria specific to tonsil in iga nephropathy patients predicts the remission by the treatment. | immunoglobulin (ig)a nephropathy (igan) is the most common form of primary glomerulonephritis in the world. some bacteria were reported to be the candidate of the antigen or the pathogenesis of igan, but systematic analysis of bacterial flora in tonsil with igan has not been reported. moreover, these bacteria specific to igan might be candidate for the indicator which can predict the remission of igan treated by the combination of tonsillectomy and steroid pulse. | 2014 | 24489644 |
| 16s rdna-based metagenomic analysis of dental plaque and lung bacteria in patients with severe acute exacerbations of chronic obstructive pulmonary disease. | acute exacerbations of chronic obstructive pulmonary disease (ae-copd) are leading causes of mortality in hospital intensive care units. we sought to determine whether dental plaque biofilms might harbor pathogenic bacteria that can eventually cause lung infections in patients with severe ae-copd. | 2014 | 24484531 |
| topical treatment with probiotic lactobacillus brevis cd2 inhibits experimental periodontal inflammation and bone loss. | an increasing body of evidence suggests that the use of probiotic bacteria is a promising intervention approach for the treatment of inflammatory diseases with a polymicrobial etiology. the objective of this study was to determine whether lactobacillus brevis cd2 could inhibit periodontal inflammation and bone loss in experimental periodontitis. | 2014 | 24483135 |
| porphyromonas gingivalis lipid a phosphatase activity is critical for colonization and increasing the commensal load in the rabbit ligature model. | periodontitis is a disease of polymicrobial etiology characterized by inflammation, degradation of host tissue, and bone that irreversibly destroys the supporting apparatus of teeth. porphyromonas gingivalis contains lipid a with structural heterogeneity that has been postulated to contribute to the initiation of dysbiosis in oral communities by modulating the host response, thereby creating a permissive environment for its growth. we examined two p. gingivalis lipid a phosphatase mutants which ... | 2014 | 24478080 |
| in vitro-activity of oily calcium hydroxide suspension on microorganisms as well as on human alveolar osteoblasts and periodontal ligament fibroblasts. | findings from animal and human studies have indicated that an oily calcium hydroxide suspension (ochs) may improve early wound healing in the treatment of periodontitis. calcium hydroxide as the main component is well known for its antimicrobial activity, however at present the effect of ochs on the influence of periodontal wound healing/regeneration is still very limited. the purpose of this in vitro study was to investigate the effect of ochs on periodontopathogenic bacteria as well as on the ... | 2014 | 24475753 |
| gingipains from porphyromonas gingivalis - complex domain structures confer diverse functions. | gingipains, a group of arginine or lysine specific cysteine proteinases (also known as rgpa, rgpb and kgp), have been recognized as major virulence factors in porphyromonas gingivalis. this bacterium is one of a handful of pathogens that cause chronic periodontitis. gingipains are involved in adherence to and colonization of epithelial cells, haemagglutination and haemolysis of erythrocytes, disruption and manipulation of the inflammatory response, and the degradation of host proteins and tissue ... | 2011 | 24466435 |
| the link between periodontal disease and rheumatoid arthritis: an updated review. | porphyromonas gingivalis is a leading pathogen in chronic periodontitis, a disease process involving progressive destruction of the tissues that support the teeth. recently, the organism has been reported to produce a unique bacterial enzyme, p. gingivalis peptidyl-arginine deiminase (ppad), which has the ability to convert arginine residues in proteins to citrulline. protein citrullination alters protein structure and function; hence, ppad may be involved in deregulation of the host's signallin ... | 2014 | 24458478 |
| periodontal disease associated with red complex bacteria in dogs. | red complex bacteria (treponema denticola, tannerella forsythia and porphyromonas gingivalis) play a major role in the aetiology of periodontal disease in humans. this study was designed to evaluate the association of such bacteria with periodontal disease in dogs. | 2014 | 24450418 |
| emerging roles of immunostimulatory oral bacteria in periodontitis development. | periodontitis is a common dental disease which results in irreversible alveolar bone loss around teeth, and subsequent tooth loss. previous studies have focused on bacteria that damage the host and the roles of commensals to facilitate their colonization. although some immune responses targeting oral bacteria protect the host from alveolar bone loss, recent studies show that particular host defense responses to oral bacteria can induce alveolar bone loss. host-damaging and immunostimulatory oral ... | 2014 | 24433922 |
| teth137, a conserved factor of unknown function from thermoanaerobacter ethanolicus jw200, represses the transcription of the adhe gene in vitro. | teth137, a 13.7 kd protein of unknown function from thermoanaerobacter ethanolicus jw200, is encoded by 360 nucleotides and has been obtained by dna-coupled column previously. however, no function study of teth137 has been published. homologous modeling of teth137 shows the protein is comprised of a helix-turn-helix motif which is a typical dna-binding domain. therefore, it is speculated teth137 is a dna-binding protein and involved in transcription of the adhe gene (encodes alcohol dehydrogenas ... | 2012 | 24426101 |
| teth137, a conserved factor of unknown function from thermoanaerobacter ethanolicus jw200, represses the transcription of the adhe gene in vitro. | teth137, a 13.7 kd protein of unknown function from thermoanaerobacter ethanolicus jw200, is encoded by 360 nucleotides and has been obtained by dna-coupled column previously. however, no function study of teth137 has been published. homologous modeling of teth137 shows the protein is comprised of a helix-turn-helix motif which is a typical dna-binding domain. therefore, it is speculated teth137 is a dna-binding protein and involved in transcription of the adhe gene (encodes alcohol dehydrogenas ... | 2012 | 24426101 |
| effects of tongue cleaning on bacterial flora in tongue coating and dental plaque: a crossover study. | the effects of tongue cleaning on reconstruction of bacterial flora in dental plaque and tongue coating itself are obscure. we assessed changes in the amounts of total bacteria as well as fusobacterium nucleatum in tongue coating and dental plaque specimens obtained with and without tongue cleaning. | 2014 | 24423407 |
| the effect of locally delivered doxycycline in the treatment of chronic periodontitis. a clinical and microbiological cohort study. | to evaluate the efficacy of locally delivered doxycycline as an adjunct to non-surgical treatment with the use of an ultrasonic device compared to scaling and root planing using hand instruments, by means of clinical and microbiological criteria. | 2010 | 24421978 |
| the effect of locally delivered doxycycline in the treatment of chronic periodontitis. a clinical and microbiological cohort study. | to evaluate the efficacy of locally delivered doxycycline as an adjunct to non-surgical treatment with the use of an ultrasonic device compared to scaling and root planing using hand instruments, by means of clinical and microbiological criteria. | 2010 | 24421978 |
| molecular identification and functional characterization of the human colonic thiamine pyrophosphate transporter. | colonic microbiota synthesize a considerable amount of thiamine in the form of thiamine pyrophosphate (tpp). recent functional studies from our laboratory have shown the existence of a specific, high-affinity, and regulated carrier-mediated uptake system for tpp in human colonocytes. nothing, however, is known about the molecular identity of this system. here we report on the molecular identification of the colonic tpp uptake system as the product of the slc44a4 gene. we cloned the cdna of slc44 ... | 2014 | 24379411 |
| molecular identification and functional characterization of the human colonic thiamine pyrophosphate transporter. | colonic microbiota synthesize a considerable amount of thiamine in the form of thiamine pyrophosphate (tpp). recent functional studies from our laboratory have shown the existence of a specific, high-affinity, and regulated carrier-mediated uptake system for tpp in human colonocytes. nothing, however, is known about the molecular identity of this system. here we report on the molecular identification of the colonic tpp uptake system as the product of the slc44a4 gene. we cloned the cdna of slc44 ... | 2014 | 24379411 |
| filifactor alocis infection and inflammatory responses in the mouse subcutaneous chamber model. | recent microbiome studies have implicated a role for filifactor alocis in periodontal disease. in this study, we investigated the colonization and survival properties of f. alocis in a mouse subcutaneous chamber model of infection and characterized host innate immune responses. an infection of 10(9) f. alocis successfully colonized all chambers; however, the infection was cleared after 72 h. f. alocis elicited a local inflammatory response with neutrophils recruited into the chambers at 2 h post ... | 2014 | 24379289 |
| haplotype analysis of interleukin-8 gene polymorphisms in chronic and aggressive periodontitis. | periodontitis is an inflammatory disease characterized by connective tissue loss and alveolar bone destruction. interleukin-8 (il8) is important in the regulation of the immune response. the aim of this study was to analyze four polymorphisms in the il8 gene in relation to chronic (cp) and aggressive (agp) periodontitis. | 2013 | 24368875 |
| periodontal bacteria in the genital tract: are they related to adverse pregnancy outcome? | one of the most important factors implicated in preterm birth (ptb) is acute genitourinary tract infection. the bacteria causing chronic periodontal inflammation include gram-negative rods and anaerobes similar to those found in women with bacterial vaginosis. the aim of this prospective study is to investigate the relationship between oral and vaginal microflora and preterm low birth weight. real-time polymerase chain reaction was used to detect both the presence and level of six periodontitis- ... | 2017 | 24355228 |
| clinical trial subjects: "panning gold". | 2013 | 24350222 | |
| the effect of adjunctive low-dose doxycycline and licorice therapy on gingival crevicular fluid matrix metalloproteinase-8 levels in chronic periodontitis. | this study compared the effect of adjunctive low dose doxycycline and licorice on gingival crevicular fluid (gcf) matrix metalloproteinase-8 (mmp-8) levels in patients with chronic periodontitis. | 2013 | 24348620 |
| dental abscess: a microbiological review. | dental abscess is a frequently occurring infectious process known to the health practice. the fate of the infection depends on the virulence of the bacteria, host resistance factors, and regional anatomy. serious consequences arising from the spread of a dental abscess lead to significant morbidity and mortality. acute dental abscess is polymicrobial, comprising of strict anaerobes, such as anaerobic cocci, prevotella, fusobacterium species, and facultative anaerobes, such as viridans group stre ... | 2013 | 24348613 |
| impact of early colonizers on in vitro subgingival biofilm formation. | the aim of this study was to investigate the impact of early colonizing species on the structure and the composition of the bacterial community developing in a subgingival 10-species biofilm model system. the model included streptococcus oralis, streptococcus anginosus, actinomycesoris, fusobacterium nucleatum subsp. nucleatum, veillonella dispar, campylobacter rectus, prevotella intermedia, porphyromonas gingivalis, tannerella forsythia, and treponema denticola. based on literature, we consider ... | 2013 | 24340084 |
| interleukin-8 responses of multi-layer gingival epithelia to subgingival biofilms: role of the "red complex" species. | periodontitis is an infectious inflammatory disease that results in the destruction of the tooth-supporting (periodontal) tissues. the gram-negative anaerobic species porphyromonas gingivalis, tannerella forsythia and treponema denticola, (also known as the "red complex" species) are highly associated with subgingival biofilms at periodontitis-affected sites. a major chemokine produced by the gingival epithelium in response to biofilm challenge, is interleukin (il)-8. the aim of this in vitro st ... | 2013 | 24339946 |
| inflammatory and immune pathways in the pathogenesis of periodontal disease. | the pathogenesis of periodontitis involves a complex immune/inflammatory cascade that is initiated by the bacteria of the oral biofilm that forms naturally on the teeth. the susceptibility to periodontitis appears to be determined by the host response; specifically, the magnitude of the inflammatory response and the differential activation of immune pathways. the purpose of this review was to delineate our current knowledge of the host response in periodontitis. the role of innate immunity, the ... | 2014 | 24320956 |
| biology and pathogenesis of cytomegalovirus in periodontal disease. | human periodontitis is associated with a wide range of bacteria and viruses and with complex innate and adaptive immune responses. porphyromonas gingivalis, tannerella forsythia, aggregatibacter actinomycetemcomitans, treponema denticola, cytomegalovirus and other herpesviruses are major suspected pathogens of periodontitis, and a combined herpesvirus-bacterial periodontal infection can potentially explain major clinical features of the disease. cytomegalovirus infects periodontal macrophages an ... | 2014 | 24320955 |
| composition and development of oral bacterial communities. | the oral bacterial microbiome encompasses approximately 700 commonly occurring phylotypes, approximately half of which can be present at any time in any individual. these bacteria are largely indigenous to the oral cavity; this limited habitat range suggests that interactions between the various phylotypes, and between the phylotypes and their environment, are crucial for their existence. molecular cataloging has confirmed many basic observations on the composition of the oral microbiome that we ... | 2014 | 24320954 |
| sequence, biophysical, and structural analyses of the psts lipoprotein (bb0215) from borrelia burgdorferi reveal a likely binding component of an abc-type phosphate transporter. | the lyme disease agent borrelia burgdorferi, which is transmitted via a tick vector, is dependent on its tick and mammalian hosts for a number of essential nutrients. like other bacterial diderms, it must transport these biochemicals from the extracellular milieu across two membranes, ultimately to the b. burgdorferi cytoplasm. in the current study, we established that a gene cluster comprising genes bb0215 through bb0218 is cotranscribed and is therefore an operon. sequence analysis of these pr ... | 2013 | 24318969 |
| sequence, biophysical, and structural analyses of the psts lipoprotein (bb0215) from borrelia burgdorferi reveal a likely binding component of an abc-type phosphate transporter. | the lyme disease agent borrelia burgdorferi, which is transmitted via a tick vector, is dependent on its tick and mammalian hosts for a number of essential nutrients. like other bacterial diderms, it must transport these biochemicals from the extracellular milieu across two membranes, ultimately to the b. burgdorferi cytoplasm. in the current study, we established that a gene cluster comprising genes bb0215 through bb0218 is cotranscribed and is therefore an operon. sequence analysis of these pr ... | 2013 | 24318969 |
| biochemical and molecular characterization of treponema phagedenis-like spirochetes isolated from a bovine digital dermatitis lesion. | bovine papillomatous digital dermatitis (dd) is the leading cause of lameness in dairy cattle and represents a serious welfare and economic burden. found primarily in high production dairy cattle worldwide, dd is characterized by the development of an often painful red, raw ulcerative or papillomatous lesion frequently located near the interdigital cleft and above the bulbs of the heel. while the exact etiology is unknown, several spirochete species have been isolated from lesion material. four ... | 2013 | 24304812 |
| crevicular fluid biomarkers and periodontal disease progression. | assess the ability of a panel of gingival crevicular fluid (gcf) biomarkers as predictors of periodontal disease progression (pdp). | 2013 | 24303954 |
| crevicular fluid biomarkers and periodontal disease progression. | assess the ability of a panel of gingival crevicular fluid (gcf) biomarkers as predictors of periodontal disease progression (pdp). | 2013 | 24303954 |
| genetic mapping in papillon-lefèvre syndrome: a report of two cases. | papillon-lefevre syndrome (pls) is a rare autosomal recessive heterogeneous trait which is characterized by erythematous palmoplantar hyperkeratosis, early-onset periodontitis, and associated calcification of dura mater. the etiology of pls is multifactorial with genetic, immunological, and microbial factors playing a role in etiopathogenesis. recently identified genetic defect in pls has been mapped to chromosome 11q14-q21, which involves mutations of cathepsin c. this paper presents a report o ... | 2013 | 24303219 |
| emerging roles of the microbiome in cancer. | gene-environment interactions underlie cancer susceptibility and progression. yet, we still have limited knowledge of which environmental factors are important and how they function during tumorigenesis. in this respect, the microbial communities that inhabit our gastrointestinal tract and other body sites have been unappreciated until recently. however, our microbiota are environmental factors that we are exposed to continuously, and human microbiome studies have revealed significant difference ... | 2013 | 24302613 |
| emerging roles of the microbiome in cancer. | gene-environment interactions underlie cancer susceptibility and progression. yet, we still have limited knowledge of which environmental factors are important and how they function during tumorigenesis. in this respect, the microbial communities that inhabit our gastrointestinal tract and other body sites have been unappreciated until recently. however, our microbiota are environmental factors that we are exposed to continuously, and human microbiome studies have revealed significant difference ... | 2013 | 24302613 |
| [relationships of five periodontal pathogens causing subgingival plaque in patients with chronic periodontitis under different periodontal conditions]. | this study aimed to assess the relationship between the distributions of haemophilus aggregatibacter (h. aggregatibacter ), porphyromonas gingivalis (p. gingivalis ), prevotella intermedia (p. intermedia), tannerella forsythensis (t. forsythensis), and treponema denticola (t. denticola) in subgingival plaque and different periodontal conditions of chronic periodontitis. | 2013 | 24298807 |
| mechanisms of il-8 suppression by treponema denticola in gingival epithelial cells. | the purpose of this study was to investigate the mechanism(s) of interleukin (il)-8 suppression by treponema denticola, one of the major periodontal pathogens, in gingival epithelial cells. immortalized human gingival epithelial hok-16b cells were infected with wild-type (wt), dentilisin-deficient (k1) or flagellin-deficient (flge) t. denticola in the presence or absence of 2% human serum for 24 h. the levels of il-8 expression were measured with real-time reverse transcription pcr and elisa. in ... | 2014 | 24296811 |
| smoking-related cotinine levels and host responses in chronic periodontitis. | smoking has been reported to increase the risk of periodontal disease by disrupting the balance of immune responses and tissue repair processes; however, this risk varies among smokers. cotinine levels in saliva are routinely used to measure the level of smoking, and reflect the quantity of nicotine, and other smoking-related xenobiotics that challenge host systems. this study delineated characteristics of inflammatory mediators in saliva and serum antibody responses to both periodontal pathogen ... | 2013 | 24283398 |
| smoking-related cotinine levels and host responses in chronic periodontitis. | smoking has been reported to increase the risk of periodontal disease by disrupting the balance of immune responses and tissue repair processes; however, this risk varies among smokers. cotinine levels in saliva are routinely used to measure the level of smoking, and reflect the quantity of nicotine, and other smoking-related xenobiotics that challenge host systems. this study delineated characteristics of inflammatory mediators in saliva and serum antibody responses to both periodontal pathogen ... | 2013 | 24283398 |
| variations in inflammatory genes are associated with periodontitis. | periodontitis is a multi-factorial disease and several risk-factors such as infections, inflammatory responses, oral hygiene, smoke, aging and individual predisposition are involved in the disease. pathogens trigger chronic inflammation with cytokines release which in turn leads to the destruction of the connective and the teeth supporting bone. the identification of genetic factors controlling oral inflammation may increase our understanding of genetic predisposition to periodontitis.single nuc ... | 2013 | 24274085 |
| functional screening and in vitro analysis reveal thioesterases with enhanced substrate specificity profiles that improve short-chain fatty acid production in escherichia coli. | short-chain fatty acid (scfa) biosynthesis is pertinent to production of biofuels, industrial compounds, and pharmaceuticals from renewable resources. to expand on escherichia coli scfa products, we previously implemented a coenzyme a (coa)-dependent pathway that condenses acetyl-coa to a diverse group of short-chain fatty acyl-coas. to increase product titers and reduce premature pathway termination products, we conducted in vivo and in vitro analyses to understand and improve the specificity o ... | 2014 | 24271180 |
| immunomicrobial pathogenesis of periodontitis: keystones, pathobionts, and host response. | recent studies have uncovered novel mechanisms underlying the breakdown of periodontal host-microbe homeostasis, which can precipitate dysbiosis and periodontitis in susceptible hosts. dysbiotic microbial communities of keystone pathogens and pathobionts are thought to exhibit synergistic virulence whereby not only can they endure the host response but can also thrive by exploiting tissue-destructive inflammation, which fuels a self-feeding cycle of escalating dysbiosis and inflammatory bone los ... | 2013 | 24269668 |
| immunomicrobial pathogenesis of periodontitis: keystones, pathobionts, and host response. | recent studies have uncovered novel mechanisms underlying the breakdown of periodontal host-microbe homeostasis, which can precipitate dysbiosis and periodontitis in susceptible hosts. dysbiotic microbial communities of keystone pathogens and pathobionts are thought to exhibit synergistic virulence whereby not only can they endure the host response but can also thrive by exploiting tissue-destructive inflammation, which fuels a self-feeding cycle of escalating dysbiosis and inflammatory bone los ... | 2013 | 24269668 |
| viscous dynamics of lyme disease and syphilis spirochetes reveal flagellar torque and drag. | the spirochetes that cause lyme disease (borrelia burgdorferi) and syphilis (treponema pallidum) swim through viscous fluids, such as blood and interstitial fluid, by undulating their bodies as traveling, planar waves. these undulations are driven by rotation of the flagella within the periplasmic space, the narrow (∼20-40 nm in width) compartment between the inner and outer membranes. we show here that the swimming speeds of b. burgdorferi and t. pallidum decrease with increases in viscosity of ... | 2013 | 24268139 |
| high incidence of aggregatibacter actinomycetemcomitans infection in patients with cerebral infarction and diabetic renal failure: a cross-sectional study. | recent epidemiological studies suggest that periodontitis is a major risk factor for renal failure and cerebral infarction. the aim of this study was to evaluate the association among periodontitis, renal failure, and cerebral infarction, focusing on microbiological and immunological features. | 2013 | 24267704 |
| the lysine gingipain adhesin domains from porphyromonas gingivalis interact with erythrocytes and albumin: structures correlate to function. | the crystal structure of the k1 domain, an adhesin module of the lysine gingipain (kgp) expressed on the cell surface by the periodontopathic anaerobic bacterium, porphyromonas gingivalis w83, is compared to the previously determined structures of homologues k2 and k3, all three being representative members of the cleaved adhesin domain family. in the structure of k1, the conformation of the most extensive surface loop is unexpectedly perturbed, perhaps by crystal packing, and is displaced from ... | 2013 | 24265933 |
| oral hygiene reinforcement in the simplified periodontal treatment of 1 hour. | to compare the clinical and microbiological outcome of the 1-h ultrasonic debridement of chronic periodontitis patients (cpp) with and without frequent sessions of oral hygiene reinforcement. | 2014 | 24255993 |
| combination of inflammatory and amlodipine induced gingival overgrowth in a patient with cardiovascular disease. | gingival overgrowth (go) is among one of the most important clinical features of gingival pathology frequently seen in periodontal clinic. amlodipine is a comparatively new calcium channel blocker and is being used with increasing frequency in the management of hypertension and angina. a 48-year-old indian woman who was on amlodipine for 3 years for hypertension reported to the department of periodontics with the complaint of swollen, un esthetic gums. the patient developed go 6 months before he ... | 2013 | 24251234 |
| microbiome and mucosal inflammation as extra-articular triggers for rheumatoid arthritis and autoimmunity. | despite the progress toward understanding the molecular pathogenesis of rheumatoid arthritis (ra), its cause remains elusive. genes are important but rather insufficient to explain the majority of ra cases. this review describes the novel data supporting the microbiome and its interactions with the human host as potential en('in')vironmental factors in ra pathogenesis. | 2014 | 24247114 |
| oral bacterial communities in individuals with type 2 diabetes who live in southern thailand. | type 2 diabetes mellitus is increasingly common in thailand and elsewhere. in the present investigation, the bacteriological composition of saliva and supragingival plaque in thai diabetics with and without active dental caries and in nondiabetics was determined by differential culture and eubacterial dna profiling. potential associations between fasting blood sugar and glycosylated hemoglobin (biomarkers of current and historical glucose control, respectively) with decayed, missing, and filled ... | 2014 | 24242241 |
| microbial changes in subgingival plaque and polymicrobial intracellular flora in buccal cells after fixed orthodontic appliance therapy: a preliminary study. | the oral ecosystem is strictly related to a balance maintained by specific niches recognized as sites, where oral bacteria can metabolize avoiding the immune system response. the oral bacteria species that colonize the ecological niches vary during fixed orthodontic treatment, with a prevalence of periodontal bacterial species. qualitative analysis of five periodontal pathogens was used to investigate the microbial colonization rate in the crevice and buccal epithelial cells. the presence of ina ... | 2013 | 24223591 |
| use of nonelectrolytes reveals the channel size and oligomeric constitution of the borrelia burgdorferi p66 porin. | in the lyme disease spirochete borrelia burgdorferi, the outer membrane protein p66 is capable of pore formation with an atypical high single-channel conductance of 11 ns in 1 m kcl, which suggested that it could have a larger diameter than 'normal' gram-negative bacterial porins. we studied the diameter of the p66 channel by analyzing its single-channel conductance in black lipid bilayers in the presence of different nonelectrolytes with known hydrodynamic radii. we calculated the filling of th ... | 2013 | 24223145 |
| metabolic proximity in the order of colonization of a microbial community. | microbial biofilms are often composed of multiple bacterial species that accumulate by adhering to a surface and to each other. biofilms can be resistant to antibiotics and physical stresses, posing unresolved challenges in the fight against infectious diseases. it has been suggested that early colonizers of certain biofilms could cause local environmental changes, favoring the aggregation of subsequent organisms. here we ask whether the enzyme content of different microbes in a well-characteriz ... | 2013 | 24204896 |
| effect of controlled-release periochip™ on clinical and microbiological parameters in patients of chronic periodontitis. | the aim of the present study was to evaluate and compare the clinical and microbiological effectiveness of periochip™ as an adjunct to scaling and root planing (srp) with srp alone in patients with chronic periodontitis. | 2013 | 24174754 |
| microbial dysbiosis in periodontitis. | periodontitis is a biofilm-associated inflammatory disease of the periodontium. this disease appears to have multiple etiologies with microbial factor contributing to initiation of the disease and immunological factor of the host propagating the disease. this review is on the concept of "microbial dysbiosis" and molecular nature of periodontitis, and the scope of traditional and emerging technologies for treating this disease. | 2013 | 24174742 |
| salivary ph: a diagnostic biomarker. | saliva contains a variety of host defense factors. it influences calculus formation and periodontal disease. different studies have been done to find exact correlation of salivary biomarkers with periodontal disease. with a multitude of biomarkers and complexities in their determination, the salivary ph may be tried to be used as a quick chairside test. the aim of this study was to analyze the ph of saliva and determine its relevance to the severity of periodontal disease. | 2013 | 24174725 |