Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| inactivation of bovine herpesvirus 1 in semen using a hyperimmune egg yolk semen extender. | hyperimmune egg yolk semen extender was used for the inactivation of bovine herpesvirus (bhv-1) in experimentally infected bovine semen. as much as 5 x 10(4) tcid50/ml of virus was inactivated in semen as assayed by tissue culture. moreover the hyperimmune egg yolk semen extender did not produce any adverse effect on the quality of the semen after being frozen/thawed in comparison with normal egg yolk semen extender (p > 0.05). the hyperimmune egg yolk semen extender is considered an important t ... | 2000 | 10780175 |
| [bvd approach: vaccination and eradication]. | the vulnerability of cattle populations that do not have adequate levels of antibodies against the bovine virus diarrhoea (bvd) virus was demonstrated in february 1999, when a contaminated vaccine against infectious bovine rhinotracheitis (ibr) was accidentally used. only cattle with antibodies against bvd survived this unintentional challenge. a bvd infection can be detected easily with currently available laboratory techniques, but the virus is less easy to eliminate. the risk of financial los ... | 2000 | 10775986 |
| [the mechanisms for latency and reactivation of alpha herpesviridae]. | alpha herpesviridae (herpes simplex virus, hsv and varicella zoster virus, vzv) is a neurotropic phathogen of humans that establishes latent infection in the sensory ganglia, and periodically reactivates from latently infected neurons. most of basic research for analyzing the mechanism of latency and reactivation of herpes viruses has been carried out in hsv. according to the results from the molecular analysis in the neurons latently infected with hsv, during latency, the virus genome is quiesc ... | 2000 | 10774199 |
| induction of nitric oxide in bovine peripheral blood mononuclear cells by bovine herpesvirus 1. | bovine peripheral blood mononuclear cells (pbmcs) and monocytes were stimulated with bovine herpesvirus 1 (bhv-1), lps and concanavalin a (con a) to produce l-arginine-dependent nitric oxide (no) in vitro. no was detected as early as 12 hrs and up to 72 hrs post stimulation (p.s.). the no from lipopolysacharide (lps)-stimulated pbmcs and monocytes was found to exhibit antiviral effect against bhv-1. the anti-bhv-1 effect was inhibited with n(omega)-methyl l-arginine indicating involvement of ind ... | 1999 | 10757233 |
| the extracellular part of glycoprotein e of bovine herpesvirus 1 is sufficient for complex formation with glycoprotein i but not for cell-to-cell spread. | glycoproteins ge and gi of bovine herpesvirus 1 (bhv-1) are type i transmembrane proteins that can form a complex that is involved in cell-to-cell spread mechanisms. the extracellular domains of both proteins have cysteine-rich regions that are also found in the homologous proteins of other alpha-herpesviruses. the extracellular domain of ge has two conserved cysteine-rich regions: c1 and c2. the other conserved regions in ge are located between c2 and transmembrane region and in the cytoplasmic ... | 2000 | 10752556 |
| risk factors for neospora caninum-associated abortion storms in dairy herds in the netherlands (1995 to 1997). | a 2 to 1 matched case control study design was used to analyze herd level risk factors for neospora caninum-associated abortion storms in 47 dairy herds. data were obtained using a questionnaire regarding the state of affairs at the farms over the 2 years prior to the abortion storm. the questionnaire included 120 variables considered to be potential risk factors for either introduction of infection or recrudescence of chronic infection. the relationship between risk factors and case control pai ... | 1999 | 10734392 |
| polymerase chain reaction amplification of genomic fragments of bovine herpesvirus-1. | especial conditions were developed for the amplification of five dna segments from us region of bhv-1 by polymerase chain reaction. in order to eliminate most nonspecific products it was found that addition of three cosolvents dmso, glycerol and np 40 was a simple method for increasing the specificity of amplification. | 2000 | 10733743 |
| virulent strain of ibr virus causes problems for scottish cattle. | 2000 | 10731067 | |
| prevalence of antibodies to ibr and bvd viruses in dairy cows with reproductive disorders. | we determined the prevalence of antibodies to infectious bovine rhinotracheitis virus (ibrv) and bovine viral diarrhea virus (bvdv) in sera of dairy cows on 4 different farms in the republic of croatia. a high percentage (60.8%) of cows had various reproductive disorders. the results showed that seroprevalence of infectious bovine rhinotracheitis (ibr) was 85.8% and that of bovine viral diarrhea (bvd) was 79.2% in tested cows. antibodies to both viruses were found in 80.8% of cows with reproduct ... | 1999 | 10729010 |
| evaluating control strategies for outbreaks in bhv1-free areas using stochastic and spatial simulation. | several countries within the eu have successfully eradicated bovine herpesvirus type i (bhv1), while others are still making efforts to eradicate the virus. reintroduction of the virus into bhv1-free areas can lead to major outbreaks - thereby causing severe economic losses. to give decision-makers more insight into the risk and economic consequences of bhv1 reintroduction and into the effectiveness of various control strategies, we developed the simulation model interibr. interibr is a dynamic ... | 2000 | 10727742 |
| supernatants of virus-infected macrophages prime uninfected macrophages for lipopolysaccharide-induced apoptosis by both an interferon-dependent and an independent mechanism. | virus-infected macrophages (m phi) release factors priming uninfected m phi for lps-induced apoptosis. this was shown for bovine m phi infected with cytopathogenic bovine virus diarrhea (bvd) virus or with bovine herpes virus-1 (bhv-1) but not for m phi infected with noncytopathogenic bvd virus. the former two viruses also induced interferon type i in m phi, which also primes macrophages for lps-induced apoptosis. however, several lines of evidence suggested that virus-infected m phi produce ifn ... | 1999 | 10725806 |
| fusion of the green fluorescent protein to amino acids 1 to 71 of bovine respiratory syncytial virus glycoprotein g directs the hybrid polypeptide as a class ii membrane protein into the envelope of recombinant bovine herpesvirus-1. | it was recently shown that the class ii membrane glycoprotein g of bovine respiratory syncytial virus (brsv) is integrated into the envelope of recombinant bovine herpesvirus-1 (bhv-1) virions in the correct orientation. to verify the hypothesis that the membrane anchor of brsv g might be suitable to target heterologous polypeptides into the membrane of recombinant bhv-1 particles, an open reading frame encoding a fusion protein between amino acids 1 to 71 of the brsv g glycoprotein and the gree ... | 2000 | 10725432 |
| homologous and heterologous interference requires bovine herpesvirus-1 glycoprotein d at the cell surface during virus entry. | expression of glycoprotein d (gd) of alphaherpesviruses protects cells from superinfection by homologous and heterologous viruses by a mechanism termed interference. we recently showed that mdbk cells expressing bovine herpesvirus (bhv)-1gd (mdbk(gd)) resist bhv-1, pseudorabies virus (prv) and herpes simplex virus-1 (hsv-1) but not the more closely related bhv-5 infection as determined by the number of plaques produced. however, the plaque size is reduced in all four viral infections suggesting ... | 2000 | 10725431 |
| bovine viral diarrhoea virus and bovine herpesvirus-1 prime uninfected macrophages for lipopolysaccharide-triggered apoptosis by interferon-dependent and -independent pathways. | the flavivirus bovine viral diarrhoea (bvd) virus exists in two biotypes, cytopathic (cp) and non-cytopathic (ncp), defined by their effect on cultured cells. cp bvd virus-infected cells undergo apoptosis and may promote apoptosis in uninfected cells by an indirect mechanism. macrophages (mφ) infected with cp, but not ncp, bvd virus release a factor(s) in the supernatant capable of priming uninfected mφ for activation-induced apoptosis in response to lipopolysaccharide. a possible role o ... | 2000 | 10725412 |
| comparison of three polymerase chain reaction methods for routine detection of bovine herpesvirus 1 dna in fresh bull semen. | five bulls were inoculated intrapreputially with bovineherpes virus 1 (bhv 1), in order to compare the relative sensitivity of three polymerase chain reaction (pcr) assays for routine diagnosis of fresh bovine semen for the presence of bhv 1 semen was collected twice a week up to 107 days post-infection (dpi). to reactivate latent virus, the bulls were treated with dexamethasone from 44 until 48 dpi. all samples were examined before and after cryopreservation treatment using a standard virus iso ... | 2000 | 10716339 |
| a ge-negative bhv1 vaccine virus strain cannot perpetuate in cattle populations. | three identical transmission experiments were successively performed to quantitatively evaluate the possible transmission of a ge-negative bovine herpesvirus 1 (bhv1) vaccine strain among cattle. after intranasal inoculation, the vaccine virus was excreted in high titers in nasal fluids. however, the vaccine virus was transmitted to only one sentinel in one experiment, and not to any of the 10 sentinel cattle in the other two experiments. based on these observations, it can be concluded that the ... | 2000 | 10715526 |
| distinctions between bovine herpesvirus 1 and herpes simplex virus type 1 vp22 tegument protein subcellular associations. | the alphaherpesvirus tegument protein vp22 has been characterized with multiple traits including microtubule reorganization, nuclear localization, and nonclassical intercellular trafficking. however, all these data were derived from studies using herpes simplex virus type 1 (hsv-1) and may not apply to vp22 homologs of other alphaherpesviruses. we compared subcellular attributes of hsv-1 vp22 (hvp22) with bovine herpesvirus 1 (bhv-1) vp22 (bvp22) using green fluorescent protein (gfp)-fused vp22 ... | 2000 | 10708447 |
| a ge-negative bovine herpesvirus 1 vaccine strain is not re-excreted nor transmitted in an experimental cattle population after corticosteroid treatments. | to study possible reactivation and to quantify subsequent transmission of a live ge-negative bovine herpesvirus 1 (bhv1) vaccine strain in cattle populations, four experiments were performed. two groups of cattle were each tested twice for the possibility of reactivation. inoculation with a ge-negative bhv1 vaccine was done either intramuscularly or intranasally and treatment with corticosteroids in an attempt to reactivate vaccine virus, was done after 6 or 11 weeks, and again after 6 months. t ... | 2000 | 10706958 |
| pneumonia induced byendobronchial inoculation of calves with bovine herpesvirus 1. | each of six calves inoculated endobronchially with bovine herpesvirus 1 (bhv-1) by means of a bronchoscope developed viral pneumonia. gross and histopathological lesions were mainly localized to the right diaphragmatic lobe (middle to caudal region) of the lung and were closely associated with the site of the deposition of the inoculum. the lesions were characterized by intranuclear inclusion bodies associated with focal necrosis of the epithelium in the lower respiratory tract. bhv-1 antigen an ... | 2000 | 10684687 |
| bovine herpesvirus 1 u(l)3.5 interacts with bovine herpesvirus 1 alpha-transinducing factor. | the bovine herpesvirus 1 (bhv-1) u(l)3.5 gene encodes a 126-amino-acid tegument protein. homologs of u(l)3.5 are present in some alphaherpesviruses and have 20 to 30% overall amino acid homology that is concentrated in the n-terminal 50 amino acids. mutant pseudorabies virus lacking u(l)3.5 is deficient in viral egress but can be complemented by bhv-1 u(l)3.5 (w. fuchs, h. granzow, and t. c. mettenleiter, j. virol. 71:8886-8892, 1997). the function of bhv-1 u(l)3.5 in bhv-1 replication is not kn ... | 2000 | 10684304 |
| a role for bovine herpesvirus 1 (bhv-1) glycoprotein e (ge) tyrosine phosphorylation in replication of bhv-1 wild-type virus but not bhv-1 ge deletion mutant virus. | bovine herpesvirus 1 (bhv-1), an alphaherpesvirus, is a major pathogen that causes respiratory and reproductive infections. we observed tyrosine phosphorylation of a 95-kda viral protein and dephosphorylation of 55- and 103-kda cellular proteins during the course of bhv-1 infection. we demonstrated bhv-1 glycoprotein e (ge) to be the tyrosine phosphorylated viral protein by immunoprecipitation. inhibition of phosphorylation of bhv-1 ge by tyrosine kinase inhibitors genistein and tyrphostin ag147 ... | 2000 | 10683338 |
| cellular expression of alphaherpesvirus gd interferes with entry of homologous and heterologous alphaherpesviruses by blocking access to a shared gd receptor. | several human and animal alphaherpesviruses can enter cells via human herpesvirus entry mediator c (hvec), a receptor for viral glycoprotein d (gd). in previous studies with cells expressing unknown entry mediators, cellular expression of alphaherpesvirus gd was shown to inhibit entry of the homologous virus and sometimes also of heterologous alphaherpesviruses. to investigate the mechanism of gd-mediated interference and the basis for cross-interference among alphaherpesviruses, hvec was expres ... | 2000 | 10683337 |
| decreased apolipoprotein c-iii concentration in the high-density lipoprotein fraction from calves inoculated with pasteurella haemolytica and bovine herpes virus-1. | lipoprotein lipid and apoprotein concentrations are known to be altered during the acute-phase response. we have previously shown that the serum activity of lecithin:cholesterol acyltransferase (lcat) and concentration of cholesteryl esters, both constituents of the high-density lipoprotein (hdl) fraction, are reduced in calves inoculated with pasteurella haemolytica and bovine herpes virus-1, the two major pathogens for calf pneumonia. the concentration of apolipoprotein c-iii (apoc-iii), a low ... | 2000 | 10676889 |
| detection of annexins i and iv in bronchoalveolar lavage fluids from calves inoculated with bovine herpes virus-1. | annexins are phospholipid-binding proteins and are abundant in the lung. annexins i and iv, but not ii and vi, have been detected in bronchoalveolar lavage (bal) fluids from calves inoculated with pasteurella haemolytica, the pathogen for calf pneumonia. in this study, bal fluids from calves with experimental pneumonia induced by inoculation to right lung lobes of bovine herpes virus-1 (bhv-1), the major viral pathogen for pneumonia, were examined for detection of annexins i and iv. of 6 calves ... | 2000 | 10676887 |
| bovine herpesvirus 1 (bhv1) seroprevalence in the breeding cattle population of the veneto region: prospects for the implementation of a control programme. | the results of a serological survey for bovine herpes virus (bhv1) antibodies in the breeding cattle population of the veneto region are presented. the data do not support the hypothesis of an high prevalence of bhv1; on farms where vaccination was not carried out most animals were seronegative, and seropositive animals were generally older. therefore, when drawing up the guidelines for a control programme, systematic immunization (with glycoprotein e-deleted vaccines) should be restricted only ... | 1999 | 10676153 |
| [heck cattle in the oostvaardersplassen: risk for infectious diseases or not?]. | this article discusses risk analysis of infectious diseases in a cattle population. heck cattle living in nature reserve 'de oostvaardersplassen' were studied as an example of the risk analysis approach. twenty-five adult cattle were tested for every infectious disease agent that the risk analysis indicated might be prevalent. all sampled cattle tested positive for bovine herpesvirus 1, whereas the prevalence of antibodies against other infectious disease agents was below the level that was assu ... | 2000 | 10666788 |
| structural aspects of the zona pellucida of in vitro-produced bovine embryos: a scanning electron and confocal laser scanning microscopic study. | structural aspects of the bovine zona pellucida (zp) of in vitro-matured (ivm) oocytes and in vitro-produced (ivp) embryos were studied in two experiments to find a tentative explanation for the zona's barrier function against viral infection. in experiment 1, the ultrastructure of the outer zp surface was studied. the diameter (nm) and the number of the outer pores within an area of 5000 microm(2) of 10 ivm oocytes, 10 zygotes, 10 8-cell-stage embryos, and 10 morulae were evaluated by scanning ... | 2000 | 10642588 |
| pseudorabies virus expressing bovine herpesvirus 1 glycoprotein b exhibits altered neurotropism and increased neurovirulence. | herpesvirus glycoproteins play dominant roles in the initiation of infection of target cells in culture and thus may also influence viral tropism in vivo. whereas the relative contribution of several nonessential glycoproteins to neurovirulence and neurotropism of pseudorabies virus (prv), an alphaherpesvirus which causes aujeszky's disease in pigs, has recently been uncovered in studies using viral deletion mutants, the importance of essential glycoproteins is more difficult to assess. we isola ... | 2000 | 10623744 |
| serological and molecular diagnosis of bovine viral diarrhoea virus and evidence of other viral infections in dairy calves with respiratory disease in venezuela. | an investigation based on 2 studies was carried out to assess the involvement of bovine virus diarrhoea virus (bvdv), bovine herpesvirus type 1 (bhv-1), and bovine respiratory syncytial virus (brsv) in calf respiratory disease in dairy farms in venezuela. in the first study, 8 farms were selected and paired serum samples from 42 calves with respiratory disease were tested by elisa for antibodies to the 3 viruses. seroconversion to bvdv, bhv-1, and brsv was found to 5, 2, and 6 farms out of the 8 ... | 1999 | 10605142 |
| particle-mediated dna immunization of cattle confers long-lasting immunity against bovine herpesvirus-1. | particle-mediated delivery was used as a method to vaccinate ruminants with a dna vaccine. the optimal conditions for gene gun-based delivery of gold particles into the epidermal layer of the skin were determined. after delivery of the gold particles, an inflammatory response was observed. this response occurred regardless of the presence of plasmid and therefore was a result of the physical disturbance of the skin by the gold particles. to identify transfected cells, a plasmid expressing a gree ... | 1999 | 10603316 |
| characterization of monoclonal antibodies against bovine herpesvirus 1 gd fusion protein expressed in e. coli. | a total of 20 hybridoma cell lines secreting monoclonal antibodies (mabs) against e. coli expressed bovine herpesvirus-1 (bhv-1) gd fusion protein were produced following the fusion of sp2/0 myeloma cells with splenocytes from balb/c mice immunized previously with immunoaffinity purified bhv-1 gd fusion protein. an indirect fluorescent antibody test (ifat) using bhv-1 infected mdbk cells was used for the selection of positive hybridomas secreting specific antibody. the monoclonal antibody isotyp ... | 1999 | 10598086 |
| improved detection of five closely related ruminant alphaherpesviruses by specific amplification of viral genomic sequences. | the detection and discrimination of five closely related ruminant alphaherpesviruses, bovine herpesvirus 1 (bhv-1), bovine herpesvirus 5 (bhv-5), caprine herpesvirus 1 (caphv-1), cervine herpesvirus 1 (cerhv-1), and rangiferine herpesvirus 1 (ranhv-1), were achieved by the development of specific pcr systems. the highly variable n-terminal of the glycoprotein c was chosen to select the diagnostic primers, except for the cerhv-1 primers, which targeted the glycoprotein d region. all the assays pr ... | 1999 | 10598083 |
| expression of bovine viral diarrhoea virus glycoprotein e2 by bovine herpesvirus-1 from a synthetic orf and incorporation of e2 into recombinant virions. | expression cassettes containing the codons for the pestivirus e (rns) signal peptide (sig) followed by a chemically synthesized orf that encoded the bovine viral diarrhoea virus (bvdv) strain c86 glycoprotein e2, a class i membrane glycoprotein, were constructed with and without a chimeric intron sequence immediately upstream of the translation start codon, and incorporated into the genome of bovine herpesvirus-1 (bhv-1). the resulting recombinants, bhv- 1/sige2(syn) and bhv-1/sige2(syn)-intron, ... | 1999 | 10580045 |
| induction of immune responses to bovine herpesvirus type 1 gd in passively immune mice after immunization with a dna-based vaccine. | the potential for plasmids encoding a secreted form of bovine herpesvirus type 1 (bhv-1) glycoprotein d (gd) to elicit immune responses in passively immune mice following intramuscular immunization was investigated. in these experiments, 6- to 8-week-old female c3h/hen or c57bl/6 mice were passively immunized with hyperimmune antisera raised against bhv-1 recombinant, truncated (secreted) gd immediately prior to immunization with plasmids. a single immunization of passively immune mice with plas ... | 1999 | 10580044 |
| significance of neospora caninum in british dairy cattle determined by estimation of seroprevalence in normally calving cattle and aborting cattle. | a case control study was conducted to evaluate the significance of neospora caninum infections in cattle in england and wales. the prevalence of n. caninum in normally calving cattle (the control group; n = 418) and aborting cattle (n = 633) was estimated using a commercial antibody-detection elisa. prevalence estimates for bovine virus diarrhoea virus, infectious bovine rhinotracheitis virus and leptospira hardjo were also obtained by serology. the prevalence of n. caninum was significantly hig ... | 1999 | 10576570 |
| serological survey for antibodies against selected infectious agents among fallow deer (dama dama) in central italy. | sera were collected from 224 fallow deer (dama dama) in reserves in central italy. samples were tested for antibodies against chlamydia spp., brucella spp. and coxiella spp. with the complement fixation (cf) test. indirect immunofluorescence was used to test for antibodies against borrelia spp. and agglutination tests were conducted for leptospira interrogans antibodies. enzyme-linked immunosorbent assay (elisa) tests were used to detect antibodies against bovine herpesvirus-1 (bhv-1) and bovine ... | 1999 | 10574071 |
| mapping t and b lymphocyte epitopes of bovine herpesvirus-1 glycoprotein b. | glycoprotein b (gb) is a major envelope protein of bovine herpesvirus-1 (bhv-1). as a subunit vaccine, the extracellular domain of recombinant gb induces neutralizing antibody and t cell responses that engender protection against virus challenge. here, lymphocytes from animals of different parentage were analysed for t cell proliferation to the gb extracellular domain for immune recognition. four truncated overlapping gb gene segments encoding 742 amino acids were expressed from a baculovirus ve ... | 1999 | 10573163 |
| reduced serum lecithin:cholesterol acyltransferase activity and cholesteryl ester concentration in calves experimentally inoculated with pasteurella haemolytica and bovine herpes virus-1. | lecithin:cholesterol acyltransferase (lcat), the enzyme responsible for esterification of cholesterol in plasma, is reported to be implicated in the regulation of inflammation in laboratory animals. the purpose of the present study was to elucidate the possible relevance of lcat in the pathogenesis of calf pneumonia induced by inoculations of pasteurella haemolytica and bovine herpes virus-1 into the calf lung. serum lcat activity was significantly (p < 0.01) reduced in calves inoculated with pa ... | 1999 | 10563286 |
| altering the cellular location of an antigen expressed by a dna-based vaccine modulates the immune response. | the potential for dna vaccines encoding mutated versions of the same antigen to modulate immune responses in c3h/hen mice was investigated. we created expression plasmids that encoded several versions of glycoprotein d (gd) from bovine herpesvirus 1, including authentic membrane-anchored glycoprotein (pslrsv.agd), a secreted glycoprotein (pslrsv.sgd), and an intracellular protein (pslrsv.cgd). immunization of an inbred strain of mice with these plasmids resulted in highly efficacious and long-la ... | 1999 | 10559338 |
| the latency-related gene of bovine herpesvirus 1 inhibits programmed cell death. | although viral gene expression occurs in the peripheral nervous system during acute infection, bovine herpesvirus 1 (bhv-1) gene expression is extinguished, many neurons survive, and latency ensues. the only abundant viral transcript expressed during latency is the latency-related (lr) rna, which is alternatively spliced in trigeminal ganglia during acute infection (l. devireddy and c. jones, j. virol. 72:7294-7301, 1998). a subset of neurons express a protein encoded by the lr gene and the lr p ... | 1999 | 10559283 |
| seroprevalence to bovine virus diarrhoea virus and other viruses of the bovine respiratory complex in venezuela (apure state). | six hundred and fifteen serum samples obtained from cows in five districts of apure state, venezuela, were tested by elisa for antibodies to bovine virus diarrhoea virus (bvdv). the same samples were also elisa-tested for antibodies to bovine herpesvirus type 1 (bhv-1) and bovine respiratory syncytial virus (brsv). additionally, the haemagglutination-inhibition (hi) test was used for detecting antibodies to parainfluenza virus type 3 (piv-3). overall, seroprevalence to bvdv was 36+/-7% (se); ser ... | 1999 | 10530426 |
| restriction endonuclease analysis of bhv-1 and bhv-5 strains isolated in argentina. | the genomes of 10 bovine herpesvirus 1 and 5 strains isolated in argentina from 1989 to 1994, recovered from animals showing different clinical signs, and two reference strains (los angeles and a663) were compared by restriction endonuclease analysis. four restriction enzymes, hindiii, bamhi, ecori and psti, were used and analysis of the restriction patterns used to assign the isolate to either the bhv-1.1, bhv-1.2 or bhv-5 genotype. there was a correlationship between restriction pattern and cl ... | 1999 | 10528541 |
| replication-defective bovine adenovirus type 3 as an expression vector. | although recombinant human adenovirus (hav)-based vectors offer several advantages for somatic gene therapy and vaccination over other viral vectors, it would be desirable to develop alternative vectors with prolonged expression and decreased toxicity. toward this objective, a replication-defective bovine adenovirus type 3 (bav-3) was developed as an expression vector. bovine cell lines designated vido r2 (hav-5 e1a/b-transformed fetal bovine retina cell [fbrc] line) and 6.93.9 (madin-darby bovi ... | 1999 | 10516020 |
| prevalence of bovine herpesvirus 1 (bhv-1) infection in hungarian cattle herds. | hungarian cattle herds were surveyed for bovine herpesvirus 1 (bhv-1) infection by elisa of milk and serum samples. in 1993, 75% of the large cattle herds (consisting of more than 50 cattle) and all small herds (small-scale producers' stocks), while in 1997 90% of the small herds were included in the survey. in the case of large herds, 79.3% of the herds and 64.1% of the samples tested were found to be positive. of the small herds, 13.5% and 15.7% tested positive in 1993 and 1997, respectively. ... | 1999 | 10497823 |
| effect of bovine herpesvirus-1 or bovine viral diarrhea virus on development of in vitro-produced bovine embryos. | in previous experiments, zona pellucida (zp)-intact in vitro-produced (ivp) embryos incubated for 1 hr with 10(6.3) tcid(50)/ml bovine herpes virus-1 (bhv-1), 10(5.3) tcid(50)/ml cytopathic (cp) bovine viral diarrhea virus (bvdv) or 10(5.3) tcid(50)/ml noncytopathic (ncp) bvdv showed no signs of virus replication or embryonic degeneration. the aims of the present study were to investigate whether a prolonged presence (24 hr or 8 days) of 10(6.3) tcid(50)/ml bhv-1 or 10(5.3) tcid(50)/ml bvdv in a ... | 1999 | 10497347 |
| diva vaccines that reduce virus transmission. | this brief review deals with the effect of diva (differentiating infected from vaccinated individuals) vaccines (also termed marker vaccines) on transmission of herpesviruses and pestiviruses in swine and cattle. pseudorabies and bovine herpesvirus 1 diva vaccines have been demonstrated to reduce transmission of wild-type virus in populations of pigs and cattle in the laboratory as well as in the field. a subunit diva vaccine based on the immunodominant e2 protein of classical swine fever virus ... | 1999 | 10486928 |
| spontaneous bhv1 recombinants in which the gi/ge/us9 region is replaced by a duplication/inversion of the us1.5/us2 region. | in a bovine herpesvirus 1 (bhv1) vaccine strain, a spontaneous bhv1 mutant (za) was found that arose from a recombination between two isomeric forms of the bhv1 genome. in this za mutant one end of the u(s) region, containing part of the us1.5 gene, was found duplicated in an inverted orientation at the other end of the u(s) region. concurrently, a 2.7 kb deletion was found in za that encompasses both the us8 (ge) and us9 gene. analysis of the in vitro growth properties of a genetically modified ... | 1999 | 10486108 |
| bovine herpesvirus 1 can infect cd4(+) t lymphocytes and induce programmed cell death during acute infection of cattle. | acute infection of cattle with bovine herpesvirus 1 (bhv-1) represses cell-mediated immunity, which can lead to secondary bacterial infections. since bhv-1 can induce apoptosis of cultured lymphocytes, we hypothesized that these virus-host interactions occur in cattle. to test this hypothesis, we analyzed lymph nodes and peripheral blood mononuclear cells (pbmc) after calves were infected with bhv-1. in situ terminal deoxynucleotidyltransferase-mediated dutp nick end-labeling (tunel) staining of ... | 1999 | 10482619 |
| the first immunoglobulin-like domain of hvec is sufficient to bind herpes simplex virus gd with full affinity, while the third domain is involved in oligomerization of hvec. | the human herpesvirus entry mediator c (hvec/prr1) is a member of the immunoglobulin family used as a cellular receptor by the alphaherpesviruses herpes simplex virus (hsv), pseudorabies virus, and bovine herpesvirus type 1. we previously demonstrated direct binding of the purified hvec ectodomain to purified hsv type 1 (hsv-1) and hsv-2 glycoprotein d (gd). here, using a baculovirus expression system, we constructed and purified truncated forms of the receptor containing one [hvec(143t)], two [ ... | 1999 | 10482562 |
| ileal and jejunal peyer's patches play distinct roles in mucosal immunity of sheep. | the majority of pathogens enter the body through mucosal surfaces and it is now evident that mucosal immunity can provide effective disease protection. however, the induction of mucosal immunity will require efficient targeting of mucosal vaccines to appropriate mucosa-associated lymphoid tissue. an animal model, based upon the surgical preparation of sterile intestinal 'loops' (blind-ended segments of intestine), was developed to evaluate mucosal and systemic immune responses to enteric vaccine ... | 1999 | 10447767 |
| transcription map and expression of bovine herpesvirus-1 glycoprotein d in early region 4 of bovine adenovirus-3. | early region 4 (e4) of bovine adenovirus type 3 (bav-3) was analyzed by northern blotting, rt-pcr analysis, cdna sequencing, and s1 nuclease protection assays. the transcriptional map of the e4 region of bav-3 has marked dissimilarities from those of mouse adenovirus-1, ovine adenovirus-287, and human adenovirus-2, for which the transcriptional maps have been constructed. the e4 region of bav-3, located between 98.6 and 89.8 mu transcribes seven distinct classes of bovine adenovirus type 3 mrna. ... | 1999 | 10441562 |
| growth behavior of bovine herpesvirus-1 in permissive and semi-permissive cells. | bovine herpesvirus-1 (bhv-1) can replicate well in bovine-derived cell lines such as madin darby bovine kidney (mdbk) but grows poorly in hamster lung (hmlu-1). virus replication, dna synthesis, and immediate-early gene expression are severely restricted in hmlu-1. we compared adsorption and penetration of bhv-1 in permissive mdbk and semi-permissive hmlu-1 cells. at a low multiplicity of infection, bhv-1 attached to permissive mdbk cells twice as much as to hmlu-1. the presence of heparin inhib ... | 1999 | 10426207 |
| evaluation of an immunofluorescent antibody test to detect bovine herpesvirus 1-specific igm. | an indirect immunofluorescent antibody test (iifat) was developed to detect bovine herpesvirus 1 (bhv-1)-specific igm. all sera were treated with protein-g agarose prior to testing to eliminate the possibility of false-positive results due to igm-isotype rheumatoid factor (igm-rf). specific igm was first detected 8 days after experimental infection of 3 calves free of maternally derived antibody, with peak responses occurring 2-7 days later. seroconversion was detected in all 3 calves using a si ... | 1999 | 10424647 |
| bhv-1 infections in styria (austria) caused by intra-community trade. | the present paper describes the prevalence of bhv-1 (bovine herpes virus 1) infections in the austrian province of styria from 1990 to 1997 and the corresponding eradication programme. based on the description of bhv-1 infections on seven holdings caused by intra-community trade, the additional guarantees granted under directive 64/432/eec (cec, 1964) are discussed and measures for improved protection of bhv-1-free regions suggested. | 1999 | 10422369 |
| health status of bulls used for natural breeding on farms in south west scotland. | one hundred and nine breeding bulls were examined during the period november 1992 to june 1993 on farms in south west scotland for evidence of infectious diseases associated with breeding. preputial washings were collected to screen for campylobacter fetus venerialis, together with serial blood samples to assess their seroprevalence to bovine virus diarrhoea virus (bvdv), bovine herpes virus-1 (bhv-1), leptospira hardjo and bovine herpes virus-4 (bhv-4). the possible impact of natural mating on ... | 1999 | 10416365 |
| bovine lymphocyte antigen-a11--specific peptide motif as a means to identify cytotoxic t-lymphocyte epitopes of bovine herpesvirus 1. | major histocompatibility complex (mhc) class i molecules present 8- to 10-mer viral peptides to antiviral cytotoxic t lymphocytes (ctls). identification of the allele-specific peptide motifs (aspms) of class i molecules enables the prediction of potential ctl epitopes of a virus from its protein sequences. based on the bovine herpesvirus 1 (bhv-1) protein sequences that conform to the bola-a11 aspm that we identified previously, potential ctl epitopes of bhv-1 were synthesized for use in cytotox ... | 1999 | 10413361 |
| antigen-specific cytokine and antibody isotype profiles induced by mucosal and systemic immunization with recombinant adenoviruses. | we investigated antigen-specific antibody and t-cell responses in mice immunized with human adenovirus type 5 (had5) vectors expressing either the authentic or truncated form of glycoprotein d (gd and tgd, respectively) of bovine herpesvirus type 1 (bhv-1). we also tested whether different routes of immunization influenced the level and type of immunity. immunization intranasally (i.n.) stimulated higher levels of gd-specific iga in the lung and nasal washes and induced a higher frequency of gd- ... | 1999 | 10413357 |
| detection of bovine herpesvirus type 1 in blood from naturally infected cattle by using a sensitive pcr that discriminates between wild-type virus and virus lacking glycoprotein e. | in the present study, we report for the first time on the detection of bovine herpesvirus type 1 (bhv-1) in whole-blood samples derived from naturally infected cattle. sensitive pcr assays specific for glycoprotein b (gb), gc, and ge of bhv-1 allow the detection of one bhv-1 dna copy in 10(5) to 10(7) peripheral blood leukocytes (pbls). the incidence of bhv-1-positive pbls in naturally infected cattle appears to be quite high (92.2% positive pbls among all samples tested), although in most cases ... | 1999 | 10405392 |
| immunogenicity and protective efficacy of a ge, gg and us2 gene-deleted bovine herpesvirus-1 (bhv-1) vaccine. | the efficacy and safety of a gene-deleted bovine herpesvirus-1 (bhv-1) vaccine was determined in a bovine herpesvirus challenge trial in calves. three different doses of the vaccine were administered intramuscularly at 10(5), 10(6) and 10(7) pfu/ml and compared to a commercial vaccine and non vaccinated control calves. challenge was performed by intranasal aerosolization with the cooper strain of bhv-1 (3 x 10(4) pfu/ml). the non-vaccinated calves shed significantly (p < 0.05) more virus than al ... | 1999 | 10403598 |
| bhv-1: new molecular approaches to control a common and widespread infection. | herpesviruses are widespread viruses, causing severe infections in both humans and animals. eradication of herpesviruses is extremely difficult because of their ability to establish latent and life-long infections. however, latency is only one tool that has evolved in herpesviruses to successfully infect their hosts; such viruses display a wide (and still incompletely known) panoply of genes and proteins that are able to counteract immune responses of their hosts. envelope glycoproteins and cyto ... | 1999 | 10390543 |
| induction of mucosal immune responses by administration of liposome-antigen formulations and interleukin-12. | we examined the effect of interleukin-12 (il-12) on the induction of mucosal immune responses following intranasal immunization with liposome-antigen formulations. we assessed the immune response to two recombinant glycoproteins (gd and gb) from bovine herpesvirus type 1 (bhv-1). positively charged liposomes induced significantly higher gd-specific iga titers than did immunization with antigen alone. this liposome formulation was selected to further assess the ability of il-12 to influence mucos ... | 1999 | 10386857 |
| improved detection of bovine herpesvirus 1 in artificially infected bovine semen by protein amplification. | infection with bovine herpesvirus 1 (bhv 1) occurs worldwide and causes serious economic losses due to loss of animals, abortions, decreased milk production, and loss of body weight. there is a real need for sensitive diagnostic procedures for detection of the presence of virus in order to achieve effective control of bhv 1-induced diseases. bhv 1 is frequently found in bovine semen and can be widely transmitted through artificial insemination. thus the detection of bhv 1 in artificial inseminat ... | 1999 | 10381088 |
| characterization of promoters integrated in the genome of bovine herpesvirus-1 (bhv-1). | bovine herpesvirus-1 (bhv-1) has been used as a vector of live recombinant vaccines for cattle which express the genes of other pathogens. because of the importance of the choice of the promoter which allows the efficient expression of the foreign genes in the bhv-1 vector, we compared the relative efficacy of various promoters integrated in the bhv-1 genome. the promoter sequences of the bhv-1 thymidine kinase (tk), gb, gc, sv40 early, and pseudorabies virus (prv) immediate early (ie) genes wer ... | 1999 | 10379933 |
| epitopes on glycoprotein c of bovine herpesvirus-1 (bhv-1) that allow differentiation between bhv-1.1 and bhv-1.2 strains. | in cattle, bovine herpesvirus-1 (bhv-1) can cause a mild genital disease known as infectious pustular vulvovaginitis (ipv) and a more severe respiratory disease known as infectious bovine rhinotracheitis (ibr). on the basis of epidemiological data, it has been proposed that these diseases are caused by strains with different genotypes (ibr by bhv-1.1 and ipv by bhv-1.2 strains). by using a panel of 237 bhv-1 isolates, a monoclonal antibody (mab 71) was found that failed to react with all 54 puta ... | 1999 | 10374966 |
| detection of bhv-1 in a naturally infected bovine fetus by a nested pcr assay. | bovine herpesvirus 1 (bhv-1) is frequently associated with abortion in naturally and experimentally infected cattle. most of the virus isolation and immunofluorescent antibody protocols described in the literature for detecting bhv-1 in bovine foetuses are rather laborious, costly and time-consuming. the detection is described of bhv-1 in the tissues of a naturally aborted bovine foetus by a nested pcr assay with no further hybridization procedures. optimal results were achieved by filtering the ... | 1999 | 10359157 |
| bovine herpesvirus type 1 glycoprotein c expression in mdbk cells and its reactivity in enzyme-linked immunosorbent assay. | glycoprotein c (gc) of bovine herpesvirus type 1 (bhv-1) is a major viral glycoprotein expressed at high level on the surface of infected cells and on the virion envelope. this glycoprotein is also a major target of immune response at both humoral and cellular levels. the plasmid prsv-gc having complete coding gene for bhv-1 gc was transfected into mdbk cells and the expression of gc in these cells was detected by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate (sds- ... | 1998 | 10358746 |
| mucosal immunization of calves with recombinant bovine adenovirus-3: induction of protective immunity to bovine herpesvirus-1. | to determine the potential of replication-competent (e3-deleted) bovine adenovirus-3 (bav-3) as a delivery system for vaccine antigens in calves, we evaluated the ability of recombinant bav-3 expressing different forms of of bovine herpesvirus-1 (bhv-1) glycoprotein gd to protect against bhv-1 infection in calves that had pre-existing bav-3 specific antibodies. three- to four-month-old calves, vaccinated intranasally with recombinant bav-3 expressing full-length gd (bav3.e3gd) or a truncated ver ... | 1999 | 10355773 |
| bovine herpes virus expressing envelope protein (e2) of bovine viral diarrhea virus as a vaccine candidate. | the gene encoding the envelope protein (e2) of bovine viral diarrhea virus (bvdv) was expressed under the thymidine kinase (tk) promoter of korean bovine herpesvirus 1 (bhv-1) isolate. thymidine kinase negative (tk-) bhv-1 recombinants expressing e2 of bvdv were constructed and the expression of e2 was identified by immunofluorescence and western blotting. compared to wild type bhv-1, the recombinant bhv-1 had a delayed cytopathogenic effect in cells. the immunogenicity of the recombinant bhv-1 ... | 1999 | 10342291 |
| modeling the effect of an outbreak of bovine herpesvirus type 1 on herd-level milk production of dutch dairy farms. | one of the impacts of disease is its effect on milk production. in the present study the effect of an outbreak of bovine herpesvirus type 1 on milk production at the herd level of certified bovine herpesvirus type 1-free dairy farms was modeled. the objective was to study several linear models to quantify the effects of a bovine herpesvirus type 1 outbreak on milk production accounting for the repeated measurements and incorporating our assumptions about the most likely duration of effects of th ... | 1999 | 10342232 |
| serological survey of viral antibodies in llamas (lama glama) in argentina. | this study analysed sera from 390 llamas (lama glama) from nine farms located in three different argentine provinces: buenos aires, cordoba and jujuy. the samples were tested for antibodies against 8 virus known to infect cattle: bovine herpesvirus type 1 (bhv-1), bovine viral diarrhea virus (bvdv), bovine adenovirus (badv iii), bovine enterovirus (bev), bovine rotavirus (brv), bluetongue virus (btv), bovine leukaemia virus (blv), and foot-and-mouth virus (fmdv) by conventional methods such as s ... | 1999 | 10337237 |
| immunization of neonates with dna encoding a bovine herpesvirus glycoprotein is effective in the presence of maternal antibodies. | neonates generally display low immune responsiveness to conventional vaccines, which may be due to the immaturity of their immune system and interference by maternal antibodies. because of the unique capacity of plasmid dna for the production of low doses of antigen over extended periods of time, we used dna immunization as an approach to induce immunity in neonates. previously, we demonstrated that a plasmid encoding a truncated secreted version of bovine herpesvirus-1 gd (tgd) induces protecti ... | 1999 | 10333244 |
| antigen-specific in vitro activation of t-lymphocyte subsets of cattle immunized with a modified live bovine herpesvirus 1 vaccine. | bovine peripheral blood mononuclear cells from cattle immunized with a modified live virus bovine herpesvirus 1 (bhv1) vaccine and from bhv1-negative cattle were incubated in vitro with inactivated bhv1 for 6 days. activation of t-lymphocyte subsets was measured by two-color flow cytometric analysis of t-cell phenotype and surface expression of cd25, the alpha-subunit of the high-affinity interleukin-2 receptor. vaccinated animals, but not unvaccinated animals, had cd3+, cd4+, and gamma-delta t ... | 1999 | 10333238 |
| susceptibility of bovine antigen-presenting cells to infection by bovine herpesvirus 1 and in vitro presentation to t cells: two independent events. | the aim of the present study was to develop an in vitro system for presentation of bovine herpesvirus 1 (bhv-1) antigens to bovine t lymphocytes and to characterize the antigen-presenting cells (apc) which efficiently activate cd4(+) t cells. two approaches were used to monitor the infection of apc by bhv-1 as follows: (i) detection of viral glycoproteins at the cell surface by immunofluorescence staining and (ii) detection of ul26 transcripts by reverse transcription-pcr. the monocytes were inf ... | 1999 | 10233945 |
| [apoptosis induced by bovine herpesvirus 1]. | the mechanism by which bovine herpesvirus 1 (bhv-1) induces apoptic cell death is not fully understood. attachment but not penetration of bhv-1 is necessary to induce apoptosis in target cells suggesting that one or more bhv-1 envelope glycoprotein(s) could be involved in the activation of the apoptotic process. in this context, we show that bhv-1 virions devoid of glycoprotein d (gd) are no longer able to induce apoptosis. in contrast, virions which contain gd in the viral envelope but do not g ... | 1998 | 10230108 |
| airborne transmission of bhv1, brsv, and bvdv among cattle is possible under experimental conditions. | to control the diseases caused by bovine herpesvirus 1 (bhv1), bovine respiratory syncytial virus (brsv), and bovine virus diarrhoea virus (bvdv), it is crucial to know their modes of transmission. the purpose of this study was to determine whether these viruses can be transmitted by air to a substantial extent. calves were housed in two separate isolation stables in which a unidirectional airflow was maintained through a tube in the wall. in one stable, three of the five calves were experimenta ... | 1999 | 10227122 |
| a specific pcr to differentiate between ge negative vaccine and wildtype bovine herpesvirus type 1 strains. | in the context of infectious bovine rhinotracheitis (ibr) control programmes using glycoprotein e (ge) deleted marker vaccines, a pcr assay was developed to allow the genotypic differentiation between wildtype bovine herpesvirus type 1 (bohv-1) and ge negative strains. this assay is based on the pcr amplification of a 281 bp dna fragment within the ge gene. the specificity of the amplification was confirmed by restriction endonuclease analysis and nucleotide sequencing of the pcr product. its ab ... | 1999 | 10227121 |
| bovine herpesvirus 1-induced apoptotic cell death: role of glycoprotein d. | bovine herpesvirus 1 (bhv-1) induces apoptotic cell death in peripheral blood mononuclear cells and in bovine b lymphoma (bl-3) cells. attachment but not penetration of bhv-1 is necessary to induce apoptosis in target cells, suggesting that one or more bhv-1 envelope glycoproteins could be involved in the activation of the apoptotic process. in the present study, we demonstrate that, although bhv-1 virions devoid of glycoprotein d (bhv-1 gd-/-) still bind to bl-3 cells, they are no longer able t ... | 1999 | 10208932 |
| cloning and expression of bovine herpesvirus-1 glycoprotein c. | glycoprotein c (gc) of bovine herpesvirus-1 (bhv-1) is expressed at high levels on surface of infected cells and on virus envelope. it is relatively immunodominant in antibody response to bhv-1 infection and protective in immunized bovines against bhv-1 challenge. in an attempt to express gc in mammalian cells, the 2.4 kb bamhi-ecori fragment, containing complete coding sequence of the gc gene was excised from a recombinant plasmid and cloned under the control of rsv-ltr. the resultant plasmid p ... | 1999 | 10205673 |
| studies of genetic relationships between bovine, caprine, cervine, and rangiferine alphaherpesviruses and improved molecular methods for virus detection and identification. | the glycoprotein b (gb) and d (gd) genes from five ruminant alphaherpesviruses, bovine herpesvirus 1 (bhv-1), bovine herpesvirus 5 (bhv-5), caprine herpesvirus 1 (caphv-1), cervine herpesvirus 1, and rangiferine herpesvirus 1, were partially sequenced. the nucleotide sequence alignments revealed a highly conserved gb gene, with homologies ranging between 87.2 and 99.6%, and a more variable gd gene, with homologies ranging between 71.3 and 98.9%. the phylogenetic analysis of the gb and gd nucleot ... | 1999 | 10203465 |
| cellular elongation factor 1delta is modified in cells infected with representative alpha-, beta-, or gammaherpesviruses. | earlier reports (y. kawaguchi, r. bruni, and b. roizman, j. virol. 71:1019-1024, 1997; y. kawaguchi, c. van sant, and b. roizman, j. virol. 72:1731-1736, 1998) showed that herpes simplex virus 1 (hsv-1) infection causes the hyperphosphorylation of translation elongation factor 1delta (ef-1delta) and that the modification of ef-1delta is the consequence of direct phosphorylation by a viral protein kinase encoded by the ul13 gene of hsv-1. the ul13 gene is conserved in members of all herpesvirus s ... | 1999 | 10196346 |
| activation of caspases and p53 by bovine herpesvirus 1 infection results in programmed cell death and efficient virus release. | programmed cell death (pcd), or apoptosis, is initiated in response to various stimuli, including virus infection. bovine herpesvirus 1 (bhv-1) induces pcd in peripheral blood mononuclear cells at the g0/g1 phase of the cell cycle (e. hanon, s. hoornaert, f. dequiedt, a. vanderplasschen, j. lyaku, l. willems, and p.-p. pastoret, virology 232:351-358, 1997). however, penetration of virus particles is not required for pcd (e. hanon, g. meyer, a. vanderplasschen, c. dessy-doize, e. thiry, and p. p. ... | 1999 | 10196272 |
| encephalitis induced by bovine herpesvirus 5 and protection by prior vaccination or infection with bovine herpesvirus 1. | calves were intranasally challenged with bovine herpesvirus 5 (bhv5) and followed for the development of viral infection, clinical encephalitis, histologic lesions in the brain, and viral sequences in the trigeminal ganglia. calves that were previously vaccinated with bovine herepesvirus 1 (bhv1, n = 4) or previously infected with bhv1 (n = 5) or that had not been exposed to either virus (n = 4) were compared. no calf developed signs of encephalitis, although all calves developed an infection as ... | 1999 | 10098684 |
| antibody response to glycoprotein e after bovine herpesvirus type 1 infection in passively immunised, glycoprotein e-negative calves. | this study was conducted to determine whether young calves with maternal antibodies against bovine herpesvirus type 1 (bhv-1) but without antibodies against glycoprotein e (ge) can produce an active antibody response to ge after a bhv-1 infection. five calves received at birth colostrum from ge-seronegative cows which had been vaccinated two or three times with an inactivated bhv-1, ge-deleted marker vaccine. after inoculation with a wild-type virulent strain of bhv-1, all the passively immunise ... | 1999 | 10097325 |
| evaluation of the effects of different trypsin treatments on semen quality after bhv-1 inactivation, and a comparison of the results before and after freezing, assessed by a computer image analyzer. | semen infected experimentally with infectious bovine rhinotracheitis virus (bhv-1) was treated with trypsin at concentrations of 0.30%, 0.25% and 0.15%, with or without (w or w/o) trypsin inhibitor in order to render the semen virus free. the trypsin treatments (at 0.30% and 0.25% by concentration) inactivating the virus up to 10(4) tcid50/ml, and its effects on semen quality were assessed weekly from the 1st to 20th week after being frozen. the following parameters were determined using a compu ... | 1999 | 10090565 |
| strains of bovine herpesvirus 1 that do not express an epitope on glycoprotein e in cell culture still induce antibodies that can be detected in a ge-blocking elisa. | two bovine herpesvirus 1 (bhv1) field strains that do not express an epitope on glycoprotein e (ge) in cell culture were inoculated into calves to examine whether their sera became positive in a ge-blocking elisa that detects antibodies against ge. this ge-blocking elisa uses one monoclonal antibody that is directed against the above mentioned epitope. all calves, except one, infected with these ge-epitope negative bhv1 strains, became positive in this ge-blocking elisa, about two weeks later th ... | 1999 | 10078594 |
| glycoprotein gl-independent infectivity of pseudorabies virus is mediated by a gd-gh fusion protein. | envelope glycoproteins gh and gl, which form a complex, are conserved throughout the family herpesviridae. the gh-gl complex is essential for the fusion between the virion envelope and the cellular cytoplasmic membrane during penetration and is also required for direct viral cell-to-cell spread from infected to adjacent noninfected cells. it has been proposed for several herpesviruses that gl is required for proper folding, intracellular transport, and virion localization of gh. in pseudorabies ... | 1999 | 10074151 |
| the effect of pre-existing adenovirus-specific immunity on immune responses induced by recombinant adenovirus expressing glycoprotein d of bovine herpesvirus type 1. | we investigated whether pre-existing adenovirus-specific immunity influenced the development of immunity to a foreign antigen expressed by recombinant adenovirus. active adenovirus-specific immunity was induced in cotton rats by i.n. administration of wild type human adenovirus type 5 (had5) two weeks before immunisation with a had5 vector expressing the glycoprotein d (gd) of bovine herpesvirus type 1 (gd-de3 recombinant adenovirus). active adenovirus-specific immunity inhibited gd-specific imm ... | 1999 | 10067700 |
| identification of murine cytotoxic t-lymphocyte epitopes of bovine herpesvirus 1. | major histocompatibility complex (mhc) class i molecules present endogenously derived viral peptides to cd8+ cytotoxic t-lymphocytes (ctls). the objective of this study was to identify the h-2dd- and h-2kd-restricted ctl epitopes of bovine herpesvirus 1 (bhv-1), based on the allele-specific peptide motifs (aspms) of the above class i molecules. nine sequences conforming to the h-2dd and h-2kd aspms were identified on bhv-1 proteins, and the respective peptides were synthesized. five of these pep ... | 1999 | 10067674 |
| construction and characterization of a glycoprotein e gene-deleted bovine herpesvirus type 1 recombinant. | to construct and characterize a recombinant glycoprotein (g)e gene-deleted bovine herpesvirus (bhv) type 1 (bhv-1). | 1999 | 10048557 |
| a chimeric protein comprised of bovine herpesvirus type 1 glycoprotein d and bovine interleukin-6 is secreted by yeast and possesses biological activities of both molecules. | bovine herpesvirus type 1 (bhv-1) glycoprotein d (gd) engenders mucosal and systemic immunity and protects cattle from viral infection. chimerization of cytokines with gd is being explored to confer intrinsic adjuvanticity on gd. addition of the appropriate cytokine may convert gd into an antigen that specifically engenders protective mucosal immunity. here dna coding for the mature bovine interleukin-6 (il-6) protein was fused through a synthetic glycine linker to the 3' end of dna coding for t ... | 1999 | 9987163 |
| bovine herpesvirus 1 requires glycoprotein h for infectivity and direct spreading and glycoproteins gh(w450) and gb for glycoprotein d-independent cell-to-cell spread. | by analogy with glycoprotein h (gh) of herpes simplex virus type 1 (hsv-1) and pseudorabies virus (prv), gh may also be essential for penetration and cell-to-cell spread of bovine herpes-virus 1 (bhv-1). this was verified with a gh-negative bhv-1 mutant (gh-bhv-1), which replicated normally on gh-expressing cells but was unable to form plaques and infectious progeny on non-complementing cells. the block in entry could be overcome by polyethylene glycol-induced membrane fusion, demonstrating that ... | 1999 | 9934684 |
| cellular expression of bovine herpesvirus 1 gd inhibits cell-to-cell spread of two closely related viruses without blocking their primary infection. | alphaherpesviral glycoprotein d (gd) is a critical component of the cell membrane penetration system. cells that express gd of herpes simplex virus type 1 (hsv1), pseudorabies virus (prv), or bovine herpesvirus type 1.1 (bhv1.1) resist infection by the homologous virus due to interference with viral entry at the level of penetration. bhv1.1 gd interferes with the distantly related viruses hsv1 and prv despite only a 30-40% sequence similarity and the complete absence of antigenic cross-reactivit ... | 1999 | 9927571 |
| reintroduction of bovine herpes virus type 1 into danish cattle herds during the period 1991-1995: a review of the investigations in the infected herds. | in denmark a programme for the systematic eradication of bovine herpes virus 1 (bhv-1) was completed during the years 1984 to 1991, but outbreaks due to new introductions of bhv-1 were seen. between january 1991 and may 1994, 22 herds became infected with bhv-1, all located closely to the german border. in 1995, 61 herds were detected bhv-1 antibody positive, but they were situated in many different parts of denmark. in order to find the source of infection owners of infected herds were intervie ... | 1998 | 9926454 |
| seroepidemiology of undifferentiated fever in feedlot calves in western canada. | the relationships between 4 bacterial and 3 viral antibody titers and morbidity (undifferentiated fever (uf)) and mortality were investigated in recently weaned beef calves. blood samples from 100 animals that required treatment for uf (cases) and 100 healthy control animals (controls) were obtained: upon arrival at the feedlot (arrival), at the time of selection as a case or control (selection), and at approximately 33 d of the feeding period (convalescent). seroconversion to pasteurella haemol ... | 1999 | 9919366 |
| compatibility of plasmids expressing different antigens in a single dna vaccine formulation. | one anticipated advantage of dna immunization is the potential to create multivalent vaccines. we have examined the effects of mixing plasmids into single formulations using plasmids expressing four different membrane bound glycoproteins from bovine herpesvirus-1 (bhv-1), bovine parainfluenzavirus-3 (bpi3) and human influenza virus (hinf). plasmids were delivered by intradermal injection into the tails of mice and the types of responses generated were clearly affected by the expressed antigen. p ... | 1998 | 9880010 |
| adaptive conjoint analysis to determine perceived risk factors of farmers, veterinarians and ai technicians for introduction of bhv1 to dairy farms. | a study was carried out to determine the possibility of a more-closed farming system for (dutch) dairy farms. the objective of the study was to provide effective and economically profitable management advice for improving the animal-health status of farms. management measures will only be successfully applied if supported by farmers and their advisors (such as veterinarians). therefore, the perception of farmers and advisors of the importance of various risk factors for the introduction of disea ... | 1998 | 9879584 |
| production and characterisation of monoclonal antibodies specific for bovine interleukin-4. | genetic immunisation is a simple method for producing polyclonal antibodies in mice. by this method, we produced antibodies against bovine interleukin-4 (boil-4). after a final injection with a recombinant boil-4 protein, nine stable hybridoma cell lines were established which secreted monoclonal antibodies (mabs) against this cytokine. specific binding of each of the mabs to recombinant boil-4 produced by escherichia coli, baculovirus, and trypanosoma brucei was demonstrated in an indirect elis ... | 1998 | 9860184 |
| expression and cellular distribution of baculovirus-expressed bovine herpesvirus 1 (bhv-1) glycoprotein d (gd) sequences. | glycoprotein d (gd) of bovine herpesvirus 1 (bhv-1), a homolog of herpes simplex virus gd, represents a major component of the viral envelope and is a dominant immunogen. to study the antigenic properties of the different regions of gd, we have expressed the full-length gd encoding gene and overlapping fragments spanning various regions of the gd open reading frame in a baculovirus (autographa californica nuclear polyhedrosis virus)--insect cell (spodoptera frugiperda, sf-9) system. maximum leve ... | 1998 | 9856100 |
| [immunobiology of bovine respiratory syncytial virus infections]. | this paper describes recent findings on the immunobiology of bovine respiratory syncytial virus (brsv) infections. the pathobiology of alveolar macrophages and brsv, and the immunological reaction of cattle to the virus after natural or experimental infection, or vaccination, were studied. because in severe cases brsv infection leads to lower respiratory tract disease, replication of brsv in alveolar macrophages was studied. alveolar macrophages, which are important aspecific defense cells in th ... | 1998 | 9836385 |
| the prevalence of antibody to the viruses of bovine virus diarrhoea, bovine herpes virus 1, rift valley fever, ephemeral fever and bluetongue and to leptospira sp in free-ranging wildlife in zimbabwe. | the prevalence of antibody to the viruses of bovine virus diarrhoea (bvd), bovine herpes virus typel (bhv1), rift valley fever (rvf), bovine ephemeral fever (bef) and bluetongue (bt) and to leptospira sp. was determined in wildlife populations in zimbabwe. evidence of infection with bvd virus was found in 14 of the 16 species examined but was greatest in eland taurotragus oryx, nyala tragelaphus angasi and bushbuck tragelaphus scriptus. persistent infection with bvd virus was found in 1 of 303 a ... | 1998 | 9825798 |