Publications

nucleotide sequence of a nuclear trna(tyr) gene from triticum aestivum.		1992	1600158
characterization of two galactosidases extracted from wheat germ with a hydroalcoholic solvent.	alpha- and beta-d-galactosidases were characterized from a hydroalcoholic extract of wheat germ (triticum vulgare). kinetic constants (vmax and km) and the optimal phs for the hydrolysis of p-nitrophenyl galactopyranosides by both enzymes were determined. these enzymes presented a high stability in hydroalcoholic medium and were inhibited by iodoacetamide and sodium p-hydroxy-mercuribenzoate.	1992	1576209
surface structure and properties of plant seed oil bodies.	storage triacylglycerols (tag) in plant seeds are present in small discrete intracellular organelles called oil bodies. an oil body has a matrix of tag, which is surrounded by phospholipids (pl) and alkaline proteins, termed oleosins. oil bodies isolated from mature maize (zea mays) embryos maintained their discreteness, but coalesced after treatment with trypsin but not with phospholipase a2 or c. phospholipase a2 or c exerted its activity on oil bodies only after the exposed portion of oleosin ...	1992	1560029
5s ribosomal gene clusters in wheat: pulsed field gel electrophoresis reveals a high degree of polymorphism.	the long-range structure of 5s rrna gene clusters has been investigated in wheat (triticum aestivum l.) by means of pulsed field gel electrophoresis. using aneuploid stocks, 5s rrna gene clusters were assigned to sites on chromosomes 1b, 1d, 5b and 5d. cluster sizes were evaluated and the copy number of 5s dna repeats was estimated at 4700-5200 copies for the short repeating unit (410 bp) and about 3100 copies for the long repeat (500 bp) per haploid genome. a comparison of wheat cultivars revea ...	1992	1557028
zinc, a neurotoxin to cultured neurons, contaminates cycad flour prepared by traditional guamanian methods.	we have used cultured ventral mesencephalic and cerebellar granule cells to test the toxicity of extracts of cycad seeds (genus cycas) and cycad-derived flours traditionally prepared in guam. there was no significant difference in the toxicity of extracts prepared from the female gametophyte tissue of c. circinalis, c. revoluta, and c. media, common wheat flour, and 13 of 17 cycad flour samples. however, extracts prepared from 4 of 17 guamanian flour samples exhibited marked dose-dependent neuro ...	1992	1556606
different patterns of rdna organization at interphase in nuclei of wheat and rye.	the physical location of the rdna repeating units (25 s, 18 s and 5.8 s rrna genes and the intergenic spacer sequences) was investigated in rye (secale cereale l.) and wheat (triticum aestivum l.) root tip meristematic cells by in situ hybridization using light and electron microscopy. the rdna sequences are organized differently in the two related and intercrossable species. in rye (2n = 14, one pair of chromosomes with nucleolar organizing regions, nors), two condensed blocks of rdna-containin ...	0	1527177
nucleotide sequence of the internal transcribed spacer region of rdna in wheat, triticum aestivum l. (gramineae).		1992	1515605
two intron-containing pre-trnas(tyr) from triticum aestivum are efficiently processed and spliced in homologous cell-free extract.	two wheat pre-trnas(tyr) containing introns and flanks are accurately and efficiently spliced in homologous wheat germ s23 extract. the initiation and termination sites upon in vitro transcription in hela cell extract have been estimated for both pre-trnas.	1992	1485489
sequence and expression of a wheat gene that encodes a novel protein associated with pathogen defense.	wheat (triticum aestivum) exhibits local acquired resistance to the powdery mildew pathogen erysiphe graminis f. sp. tritici. the resistant state can be induced by a preinoculation with the nonhost pathogen e. g.f. sp. hordei, the barley powdery mildew, and is accompanied by the activation of putative defense genes. here, we report the sequence of a pathogen-induced gene, wir1a, and a corresponding cdna, wir1, that encode novel defense-related proteins of 88 and 85 amino acids, respectively. ana ...	1992	1477405
fructo-oligosaccharides from the stems of triticum aestivum.	fructo-oligosaccharides, extracted with hot water from wheat stems before flowering, were fractionated by gel-permeation chromatography on biogel p2. the tri-/tetra- and penta-saccharide fractions were purified by hplc and analysed by the reductive cleavage method. the trisaccharides 1-kestose and 6-kestose, the branched tetrasaccharide bifurcose, and the (2-->6)-linked pentasaccharide were identified. the fractions of higher molecular weight were also investigated and confirmed the branched-lev ...	1992	1473106
histochemistry of glandular metaplasia at the trigone of the urinary bladder in cows.	the epithelium at the trigone of the urinary bladder showed intestinal metaplasia of a colonic type in three holstein-friesian cows affected with chronic polypoid cystitis. except for ricinus communis (rca-i), almost all goblet cells in the whole crypt were positive to periodic acid-schiff (pas), alcian blue (ab) ph 2.5, ab ph 1.0, periodate borohydride-potassium hydroxide-pas (pb-koh-pas), ulex europaeus (uea-i), triticum vulgare (wga) and arachis hypogaea (pna) after neuraminidase digestion. t ...	1992	1452812
glycaemic index of conventional carbohydrate meals.	the glycaemic index (gi) and the triacylglycerol response were measured in thirty non-insulin-dependent diabetes mellitus patients given 50 g portions of five different conventional indian meals containing semolina (triticum aestivum) cooked by two different methods, or combinations of semolina and pulse (black gram dhal (phaseolus mungo), green gram dhal (phaseolus aureus) or bengal gram dhal (cicer arietum)). there were no significant differences among meals in mean gi except for meals based o ...	1992	1445824
rapid separation of tyrosine-specific trna from white lupin.	during isolation of total ribonucleic acids from white lupin (lupinus albus) and their subsequent separation by 10% polyacrylamide gel electrophoresis, a fast migrating rna band is very well separated. the nucleotide sequence analysis of 76 nucleotide long sequence with many modified nucleosides was found to be identical with that of tyrosine specific trna of yellow lupin seeds (lupinus luteus) and wheat germ (triticum aestivum). also this trna(tyr) is identical with plant amber suppressor trna. ...	1992	1441849
sensory evaluation of and acceptability trials on biscuits prepared from raw and malted wheat (triticum aestivum)-bengal gram (cicer arietinum) mixes with or without a green leafy vegetable.	biscuits were prepared by varying the amounts of the basic ingredients to arrive at the most acceptable recipe. the final recipe contained 40 g of mix, 40 g of jaggery and 20 g of ghee. sensory evaluation of biscuits containing 5 or 10% colocasia leaf powder by composite scoring test and hedonic scale showed that the former type of biscuit was preferred over the latter. the acceptability trial conducted on 42, 3 to 6 year old children showed that biscuits from either malted mix with or without 7 ...	1992	1438072
enhanced stability of histone octamers from plant nucleosomes: role of h2a and h2b histones.	gel filtration and sedimentation studies have previously established that the vertebrate animal core histone octamer is in equilibrium with an (h3-h4)2 tetramer and an h2a-h2b dimer [eickbush, t. h., & moudrianakis, e. n. (1978) biochemistry 17, 4955-4964; godfrey, j. e., eickbush, t. h., & moudrianakis, e. n. (1980) biochemistry 19, 1339-1346]. we have investigated the core histone octamer of wheat (triticum aestivum l.) and have found it to be much more stable than its vertebrate animal counte ...	1992	1420197
effect of alpha-gliadin-derived peptides from bread and durum wheat on k562(s) cells.	previous studies suggested that the proteins and peptides that are responsible for coeliac small intestinal lesions, are also able to agglutinate k562(s) cells. on the contrary, peptides from whole gliadins from durum (tetraploid) wheat do not agglutinate these cells. bread wheat alpha-gliadins have been identified as a major toxic fraction in coeliac disease. in the present research, alpha-gliadins were purified from durum and bread wheat cultivars. alpha-gliadin peptides from bread wheat were ...	1992	1392022
variations in chloroplast proteins and nucleotide sequences of three chloroplast genes in triticum and aegilops.	two alloplasmic wheat lines having the same common wheat nucleus but the cytoplasms of aegilops crassa and ae. columnaris together with the corresponding normal line (control) were used in the two-dimensional gel electrophoresis of soluble and thylakoid membrane proteins of the chloroplast. three chloroplast polypeptides: the rubisco large subunit, the beta subunit of atp synthase, and an unidentified 31 kda protein, differed in the common wheat and two aegilops cytoplasms. three chloroplast gen ...	1992	1388032
block-surface staining for differentiation of starch and cell walls in wheat endosperm.	a staining technique for differentiating starch granules and cell walls was developed for computer-assisted studies of starch granule distribution in cells of wheat (triticum aestivum l.) caryopses. blocks of embedded caryopses were sectioned, exposing the endosperm tissue, and stained with iodine potassium iodide (iki) and calcofluor white. excessive tissue hydration during staining was avoided by using stains prepared in 80% ethanol and using short staining times. the iki quenched background f ...	1992	1376154
construction of a chromosome-enriched hpaii library from flow-sorted wheat chromosomes.	we report here the first successful generation of a chromosome-enriched library from flow sorted plant chromosomes. chromosomes with a characteristic dna content (a peak) were sorted from a synchronized cell culture (takb1, derived from triticum aestivum). a hpaii library was constructed from the sorted chromosomes and half of the cloned dna sequences analysed are unique or low copy. approximately half of these sequences when used as probes detect sequences on wheat chromosome 4a. the generation ...	1992	1374560
metabolic response to treatment with cold, paraquat, or 3-amino-1,2,4-triazole in leaves of winter wheat.	we treated leaves of winter wheat (triticum aestivum l.) with cold, paraquat, or 3-amino-1,2,4-triazole and compared the responses. we assayed the activities of glucose-6-phosphate dehydrogenase, catalase, dehydroascorbate reductase and ascorbate free radical reductase and levels of hydrogen peroxide, glucose-6-phosphate, fructose-6-phosphate, ascorbate, dehydroascorbate, reduced and oxidized glutathione. with any of the three treatments, contents of cellular peroxides and hexose phosphates were ...	2005	1369090
toward a cytogenetically based physical map of the wheat genome.	bread wheat (triticum aestivum l. em thell) is well suited for cytogenetic analysis because the genome, buffered by polyploidy, can tolerate structurally and numerically engineered chromosomes for analysis over infinite generations. this feature of polyploidy can be used in developing a high-resolution, cytogenetically based physical map of the wheat genome. we show that numerous deletions, observed in the progeny of a monosomic addition of a chromosome from triticum cylindricum in wheat, result ...	2006	1360666
absence of guar efficacy in complex spaghetti meals on postprandial glucose and c-peptide levels in healthy control and non-insulin-dependent diabetes mellitus subjects.	the effects of guar incorporated into a complex spaghetti meal on the glycaemic response in 11 healthy and 6 non-insulin dependent diabetic (niddm) subjects was studied. to this end, subjects consumed spaghetti made of either triticum aestivum or triticum durum wheat with or without 20 g% guar, as part of a complex meal containing 27% fat, 19% protein and 51% carbohydrate. in both the healthy as well as the niddm subjects the incremental integrated postprandial glucose and c-peptide responses af ...	1992	1337059
diacylglycerol kinase in plasma membranes from wheat.	diacylglycerol kinase activity was demonstrated in highly purified plasma membranes isolated from shoots and roots of dark-grown wheat (triticum aestivum l.) by aqueous polymer two-phase partitioning. the active site of the diacylglycerol kinase was localized to the inner cytoplasmic surface of the plasma membrane using isolated inside-out and right-side-out plasma membrane vesicles from roots. the enzyme activity in plasma membrane vesicles from shoots showed a broad ph optimum around ph 7. the ...	1992	1310876
amino acid composition of the endosperm free amino acids and proteins from a maturing common wheat and its extracted tetraploid.		2008	1270689
[synthesis of analogues of acetohydroxam lacetic acid with phytotoxic activity].	syntheses, spectroscopic data and phytotoxic activities of some n-acyl-o-alkylhydroxylamines, similar in structure to the natural compoud, methyl acetohydroxamyl acetate, are reported. many of synthesized compounds show strong growth-inhibiting activity on both roots and coleoptiles of triticum vulgare seedlings.	1976	1253963
analysis of the genome of plants. ii. characterization of repetitive dna in barley (hordeum vulgare) and wheat (triticum aestivum).	barley and wheat dnas have been characterized by studying their kinetics of reassociation, melting properties and sedimentation behaviour in neutral cscl gradients as well as in cs2so4 gradients containing ag+ or hg2+. in both species, reassociation kinetics have revealed the presence of approx. 76% redundant nucleotide sequences which have been grouped into very rapidly reassociating (cot 0-0.01), rapidly reassociating (cot 0.01-1.0) and slowly reassociating (cot 1-100) fractions. the barley co ...	1976	1247616
[investigation on wheat varieties of better protein quantity and quality (author's transl)].	a research has been conducted on several hundreds of cultivars and selection lines of bread wheat to find out new varieties of better nutritive characteristics. for a coarse evaluation of protein quantity and quality, a screening method (dyebinding capacity, dbc) was utilized. on the same samples, nitrogen was determined also by the kjeldahl method; in some cases aminoacids analysis was carried out. a very high correlation was found between protein content and dbc and, even higher in this case, ...	2013	1243931
structural studies on wheat (triticum aestivum) proteins lacking phenylalanine and histidine residues.	1. three very similar proteins, each of approx. 120 amino acid residues but lacking phenylalanine and histidine, were isolated from wheat (triticum aestivum) flour in sufficient quantities for further structural studies. 2. each protein, after reduction and carboxymethylation, was cleaved at the three methionine residues with cnbr to give four major peptides, which were isolated. these peptides are suitable for future sequencing studies, as the sums of their amino acid compositions are in good a ...	2003	1201000
wheat protein.	results from aid-supported nebraska research to improve the nutritional quality of wheat indicate that substantial genetic variation for grain protein content exists in wheat. experimental lines with 5% higher protein in their grain than ordinary varieties have been selected from a high-protein x high-protein cross. genetic variablity for lysine in wheat grain is limited. the genetic component of total lysine variability among 12,6000 wheats in the usda world collection was only 0.5%. genetic in ...	2013	1032110
quantitative genetically nonequivalent reciprocal crosses in cultivated plants.	quantitative expressions of character difference between reciprocal crosses have been studied by different researchers in a number of plant species, such as epilobium, zea mays, oryza sativa, hordeum sativum, triticum aestivum, trifolium hybridum, linum usitatissimum, nicotiana rustica, and others. in all cases it was found that the nonequivalence of reciprocal crosses manifested itself beginning with the f1 generation, with the exception of some flax crosses in which reciprocals differed beginn ...	2015	1032105
diallel analysis of four agronomic traits in common wheat.	the common wheat cultivars aköz, florence, mentana, jaral, and siete cerros were crossed in 1972 in all possible combinations excluding reciprocals. in 1972--1973 the f1 hybrids and the five parents were field grown at bornova in randomized complete blocks. the characters studied were plant height, spike length, 1000-kernel weight, and plant yield. the analysis of data showed that (a) with respect to plant height and 1000-kernel weight all f1 combinations deviated from the corresponding midparen ...	2008	1032104
heritability estimates from four generations of a diallel cross of common wheat.	the heritabilities of 10 quantitative characters were studied on f1, f2, backcross, and selfed backcross generations of a five-parent diallel cross of common wheat grown at two locations. from this study it was found that (a) most of the characters in question were highly heritable, (b) the heritabilities estimated by regression and variance component methods were more or less the same (r=0.84 to 0.95), and (c) the heritability of some characters was affected by location or by generation; in ...	2008	1032103
evaluation of branched ear derivatives of triticum aestivum l.	the branched spikes of triticum turgidum l. have a potential of producing a high number of kernels. some of the selected lines of t. turgidum yielded up to 150 kernels per spike as compared to 60--70 kernels per spike in the common wheat cultivar chenab-70. the best of these lines were crossed with the common wheats chenab-70, mexipak, and c-271. among the lines obtained by selection from the advanced generations of the t. turgidum x chenab-70 cross, some are similar to chenab-70 with respect to ...	2002	1032097
formation of root hairs by the wheat triticum vulgare host. trichoblasts.	the process of root hair formation has been studied by light-, transmission-and scanning electron microscopy. in the course of root hair development a break of the outer cell wall is observed by electron microscopy. it apparently occurs after the break of the fibrillar layer. the break of the outer layer of the cell wall is assumed to represent the break of the outer mucilage, the cuticle and the adjoining amorphous matrix with irregularly oriented cellulose microfibrils. the scheme of successiv ...	1976	1025984
identification of wheat (triticum aestivum l.) chromosomes with genes controlling the level of nitrate reductase, nitrite reductase, and acid proteinase using the chinese spring-hope substitution lines.	the levels of nitrate reductase, nitrite reductase, and acid proteinase were compared in the primary leaves of 8-day-old wheat seedlings of chinese spring, hope, and the 21 disomic substitution lines of hope in chinese spring. two chromosomes, 7b and 7d, were considered to contain genes controlling the level of nitrate reductase. substitution of hope chromosome 7b caused a highly significant increase in the in vitro stability of nitrate reductase. nitrite reductase appeared to be controlled by t ...	1976	1016225
translocation and metabolic fate of monosodium methanearsonic acid in wheat (triticum aestivum l).		2002	1002900
developmental specificity and evolution of the acid phosphatase isozymes of triticum aestivum and its progenitor species.	the tissue and developmental specificities of the acid phosphatase (acph) isozymes of triticum aestivum and its progenitor species t. turgidum and t. tauschii have been determined and compared using the zymogram technique. tissue and/or developmental variation in relative staining intensity, suggestive of variation in the quantity of active enzyme present, was observed for each of the seven major isozymes expressed. isozymes homologous to each of the major isozymes of the hexaploid were detected ...	1976	985382
[pollen development in the anthers of several cereal strains and hybrids during cultivation in vitro].	in order to induce androgenesis in vitro anthers of some cereals were cultivated. the highest number of proembryos was obtained in the hybrid triticale in f3 generation on blayder's medium supplemented with 3 and 12% sucrose. proembryos represented multi-nuclear and multicellular formations which stagnated at the globular stage of development. origin of roots from calluses was not accompanied by formation of buds. there was no formation of embryos from pollen grains in case of lines triticum aes ...	0	982576
wheat germ agglutinin. evidence for a genetic basis of multiple forms.	germ from hexaploid wheat (triticum aestivum l.) contained three forms of agglutinin separable by ion-exchange column chromatography at ph 3.8, while germ from tetraploid wheat (triticum turgidum l. (durum group)) contained only two such forms. the different number of forms, not due to protein modification occurring during the purification process, was demonstrable in commercial germ and in bran fractions containing germ from single wheat varieties. this evidence for a genetic basis of lectin mu ...	1976	953023
location of chromosomal control of ga-induced enzyme release by distal half-grains of wheat.	the three enzymes, alpha-amylase, peroxidase, and acid phosphatase, are among those released by the aleurone of bread wheat, triticum aestivum, as a direct response to gibberellins (ga) from the embryo during germination. aneuploid genotypes are used to investigate the chromosomal location, nature, and extent of genetic control of the release of these enzymes in endosperms after induction by exogenous ga. ditelosomics demonstrate the effect of removal of known chromosome arms, and tetrasomics sh ...	1977	849252
conserved message of o-diphenolase in wheat embryos (triticum aestivum).		1975	812720
phosphorylation of ribosomal proteins from eukaryotes in homologous and heterogous cell-free systems.	the phosphorylation of ribosomal proteins from eukaryotes in homologous and heterologous cell-free systems has been studied. the ribosomes and protein kinases from yeast (saccharomyces cerevisiae, strain bu), wheat (triticum vulgare) and rabbit (orystolagus cuniculus) have been used. it has been found that five ribosomal proteins incorporate gamma-32p from atp during the incubation of wheat ribosomes with wheat protein kinase. when the phosphorylation of isolated wheat ribosomal proteins was exa ...	1976	764872
genetic aspects of wheat gliadin proteins.	inheritance of gliadin components unique to three different varieties of common wheat (triticum aestivum l.) was studied in f1 and f2 seeds of intervarietal crosses using protein patterns obtained by polyacrylamide gel electrophoresis in aluminum lactate buffer (ph 3.2). the patterns of f1 seeds of the crosses cheyenne x justin and inia 66r x justin evidenced all the bands present in the patterns of the parents; band intensities reflected gene dosage levels dependent on whether the contributing ...	1978	728070
alterations in cell surface membrane components of adapting rat small intestinal epithelium. studies with lectins after massive proximal jejunoileal resection and jejunoileal transposition.	after proximal resection or transposition of intestinal segments, the small intestinal mucosa of rats was examined with fluorescein-conjugated lectins derived from ricinus communis and triticum vulgare (wheat germ). these agglutinins are thought to be specific for the nonreducing end-terminal carbohydrate residues, beta-d-galactose and beta-n-acetyl-d-glucosamine, respectively. after 70% proximal jejunoilealal resection as well as transposition of ileal segments to jejunum, ileal villus cell sur ...	1978	710859
some characteristics of protein disulfide isomerase (e.c.5.3.4.1) from wheat (triticum vulgare) embryo.		1978	698293
analysis of plant genomes. iii. denaturation and reassociation properties of cryptic satellite dnas in barley (hordeum vulgare) and wheat (triticum aestivum).	a cryptic satellite fraction was isolated from barley and wheat by preparatory ultracentrifugation of total dna in ag+-cs2so4 density gradients and was characterized by studying its denaturation-reassociation properties. wheat satellite dna underwent thermal denaturation as a single component with a tm of 81 degrees c while barley satellite dna consisted of one major (tm = 82.5 degrees c) and one minor (tm = 91 degrees c) component. when the barley and wheat satellites were reassociated and then ...	1978	698222
low-intensity two-step absorption of chlorophyll a in vivo.	the intensity-dependent transmission of primary leaves of triticum aestivum seedlings at lambda = 694 nm was measured with single pulses of a q-switch ruby laser. at photon flux densities above 2 x 10(17) cm-2s-1 a decrease of transmission was observed. the result is interpreted as a two-step absorption of cooperative units of 10(5)-10(6) chlorophyll molecules.	1978	623865
[effects of pipobroman and other piperazine derivatives on mitotic activity and nucleic acids of root meristems of wheat (triticum aestivum l.) ii. biochemical studies].		1977	602799
[effects of pipobroman and other piperazine derivatives on mitotic activity and nucleic acids of root meristems of wheat (triticum aestivum l.) i. cytological studies].		1977	602798
polyploidy and domestication: the origin and survival of polyploids in cytotype mixtures.	the origin and survival of a polyploid in a mixture of this polyploid and its parent(s) is reviewed. with several examples a picture is drawn of the interference of cytotypes in a mixture of cytotypes. some natural polyploids, both wild and domesticated, are very successful. they, like bread wheat and banana, largely replaced their parents. the same is true for some artificial polyploids like autotriploid hybride sugar beet in europe and autotetraploid perennial ryegrass. but when grown together ...	1979	550834
[lectins as reagents for the differentiation of serum enzymes. lectins as reagents, i. (author's transl)].	lectins from canavalia ensiformis, phaseolus vulgaris, and triticum vulgare react with arylamidase, alkaline phosphatase, gamma-glutamyltransferase, and cholinesterase of human sera by formation of enzymatically active, mostly insoluble complexes. arylamidase, alkaline phosphatase, and cholinesterase react more intensely in sera of healthy people than in sera of patients with liver and neoplastic diseases. arylesterase is bound to a distinct degree only by concanavalin a. the enzymes mentioned a ...	1979	547035
wheat-germ aspartate transcarbamoylase. steady-state kinetics and stereochemistry of the binding site for l-aspartate.	1. the steady-state kinetics of the bisubstrate reaction catalysed by aspartate transcarbamoylase purified from wheat (triticum vulgare)-germ have been studied at 25 degrees c, ph 8.5 and i 0.10-0.12. initial-velocity and product-inhibition results are consistent with an ordered sequential mechanism in which carbamoyl phosphate is the first substrate to bind, followed by l-aspartate, and carbamoyl aspartate is the first product to leave, followed by pi. the order of substrate addition is support ...	1979	534495
[electron microscopic study of the mitochondria in the apical meristem of a wheet shoot in ontogeny].	the mitochondria of apical meristem cells in the wheat (triticum aestivum l.) shoot were studied during ontogenesis using electron microscope and morphometrical methods. changes in their structure were followed from the juvenile mitochondria of the seed embryonic ear cells. the parameters of the "average" mitochondrion, such as profile area, outer membrane length, were shown to differ relatively weakly during the periods with different meristem activity. changes in the internal structure of the ...	1979	492656
the amino acid sequence of ferredoxin from triticum aestivum (wheat).	the amino acid sequence of the ferredoxin of triticum aestivum (wheat) was determined by using a beckman 890c sequencer in combination with the dansyl--phenylisothiocyanate method to characterize peptides obtained by tryptic, chymotryptic and thermolytic digestion of cnbr-cleavage fragments. the molecule consists of a single polypeptide chain of 97 residues and has an unblocked n-terminus. it shows considerable similarity to other plant-type ferredoxins.	1979	486088
plant histone 2 from wheat germ, a family of histone h2a variants. partial amino acid sequences.	1. the 0.5 m perchloric acid extract prepared from chromatin of wheat germ, triticum aestivum, contains a group of histones formerly called plant histones. these can be resolved by gel filtration on bio-gel p-60 with subsequent cm-cellulose ion-exchange chromatography into five histone fractions containing families of histones h2a and h2b. 2. the partial amino acid sequences of histone h2a variants h2a(1)triticum, h2a(2)triticum and h2a(3)triticum are presented. extensive sequence homology exist ...	1979	454665
[additional limitations of the shrikhande method and their verification].	shrikhande's method was checked on linear-variety common wheat lutescens 62. two causes for a distortion of the ratio of genotypic and paratypic components of the variance were established: parameter b of smith's law is not constant for a given field and depends on the method of parcelling: smith's law is not confirmed at all. a two-stage test of shrikhande's method correctness is suggested for checking its limitations in practice. the analysis of variance for any given parcelling is done at the ...	2008	356360
modified chromogenic alpha-amylase assay for sprouted wheat.	sensitivity of a test in which a commercially available substrate was used for the assay of alpha-amylase in sprouted wheat has been increased by the use of optimally buffered (ph 6.20) cibacron-blue dyed amylose at 60 degrees c. with the modified test, measurable alpha-amylase levels were in the range of 3--30 millidextrinizing units (mdu) as compared to the 10--90 mdu range in a test previously reported for bread wheats. the modification extends the use of the test to evaluation of sprout dama ...	1979	311358
influence of homoeologous chromosomes on gene-dosage effects in allohexaploid wheat (triticum aestivum l.).	gene-dosage responses for a group of six wheat endosperm proteins have been investigated by using compensated nulli-tetrasomic lines of cv. chinese spring. practically linear dosage responses have been observed for all the proteins. however, for two of the proteins (and probably for a third one), the net output of protein, at each dosage of its structural gene, was 30-80% higher when the chromosome carrying an active homoeogene was absent. the possible significance of this effect in connection w ...	1977	274731
properties of common wheat ferredoxin, and a comparison with ferredoxins from related species of triticum and aegilops.	wheat ferredoxin was purified from the leaves of common wheat (triticum aestivum). the absorption spectrum showed maxima at 465, 425, 332, and 278 nm. the absorbance ratio, a425 nm/a278 nm was 0.49, and the millimolar extinction coefficient at 425 nm was 10.8 mm-1. cm-1. the amino acid composition was determined to be lys5, his2, arg1, asp11, thr5, ser7, glu18, pro5, gly6, ala7, cys5, val7, met1, ile4, leu7, tyr4, phe1, and trp1. the total number of amino acid residues was 97. the molecular weig ...	1979	110795
investigations on the biological activity of tylopilus felleus (bull. ex fr.) p. karst. by means of phytobiological tests.	the effect of the undialysed (nd) aqueous fraction prepared from the fruitbodies of tylopilus felleus and of actinomycin c on the biosynthesis of dna in the seedling roots of triticum vulgare and on the increase of their mass was investigated. the content of dna (expressed in mug) in the determined amount of the seedling roots of triticum vulgare after treating of material with the nd fraction, was 9-1--23-3% higher than in the control. the investigated fraction inhibited the increase of the mas ...	2013	54908
wheat-germ aspartate transcarbamoylase. purification and cold-lability.	1. aspartate transcarbamoylase was purified approx. 3000-fold from wheat (triticum vulgare) germ in 15-20% yield. the product has a specific activity of 14 mumol/min per mg of protein and is approx. 90% pure. the purification scheme includes the use of biospecific "imphilyte" chromatography as described by yon [biochem.j.(1977) 161, 233-237]. the enzyme was passed successively through columns of cpad [n-(3-carboxypropionyl)aminodecyl]-sepharose in the absence and presence respectively of the lig ...	1979	43131
effect of benzimidazole on nicotinamide adenine dinucleotide phosphate phosphomonoesterase activity in wheat leaves.	nicotinamide adenine dinucleotide phosphate phosphomonoesterase was isolated and partially purified from wheat (triticum aestivum l. var. selkirk) leaves. the enzyme had knadp value of 1.4 x 10(-4) m and a ph optimum of 5.9. in vitro activity of this enzyme was unaffected by precursors of nad (nicotinamide and nicotinic acid) or cytokinins (kinetin and benzimidazole). however, when detached wheat leaves were treated with solutions of these compounds, the precursors lowered the specific activity ...	1976	9313