Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| salmonella enterica serovar typhi: molecular analysis of strains with decreased susceptibility and resistant to ciprofloxacin in india from 2001-2003. | chromosomally-mediated reduced susceptibility to ciprofloxacin narrows the therapeutic options in enteric fever. we made a molecular comparison of clinical isolates of fluoroquinolone-resistant strains of salmonella enterica serotype typhi from january 2001 to may 2003; 178 isolates were subjected to antimicrobial susceptibility testing by the kirby-bauer method of disk diffusion, and agar dilution was used to determine the minimum inhibitory concentration (mic) to ciprofloxacin. nalidixic-acid ... | 2007 | 17873998 |
| flagella facilitate escape of salmonella from oncotic macrophages. | the intracellular parasite salmonella enterica serovar typhimurium causes a typhoid-like systemic disease in mice. whereas the survival of salmonella in phagocytes is well understood, little has been documented about the exit of intracellular salmonella from host cells. here we report that in a population of infected macrophages salmonella induces "oncosis," an irreversible progression to eukaryotic cell death characterized by swelling of the entire cell body. oncotic macrophages (onmphis) are t ... | 2007 | 17873035 |
| prolonged treatment of salmonella enterica serovar typhimurium with commercial disinfectants selects for multiple antibiotic resistance, increased efflux and reduced invasiveness. | to study how disinfectants affect antimicrobial susceptibility and phenotype of salmonella enterica serovar typhimurium sl1,344. | 2007 | 17855722 |
| structural characterization and serological specificities of lipopolysaccharides from salmonella enterica serovar gallinarum biovar pullorum standard, intermediate and variant antigenic type strains. | the structure and serological specificities of the lipopolysaccharides (lpss) from salmonella enterica serovar gallinarum biovar pullorum were studied to provide an improved basis for the distinction between antigenic types and the development of improved diagnostic tests. the structure of the lps o-polysaccharide (o-ps) from s. pullorum standard, intermediate and variant antigenic type strains was determined by mass spectrometry, nuclear magnetic resonance spectroscopy and chemical analysis. th ... | 2008 | 17855026 |
| clonal diversity of salmonella enterica serotype typhi isolated from patients with typhoid fever in tehran. | in this study, antimicrobial susceptibility test and genetic typing were used to characterize 15 salmonella enterica serotype typhi (s. typhi) isolates recovered from sporadic cases of typhoid fever in tehran, iran during 2004. antimicrobial susceptibility test showed that all isolates were susceptible to 20 antimicrobials examined in this study. analysis of insertion elements showed that 2 is200 types with 10 and 11 copies were present. 11 of the 15 isolates were found to possess 10 is200 eleme ... | 2008 | 17852914 |
| influence of dietary catechols on the growth of enteropathogenic bacteria. | the dietary constituents that may act, in the broadest sense, as co-factors to enable bacterial enteropathogens to replicate in gastrointestinal environments are still largely unknown. recent work has demonstrated that certain non-nutritional components of food, such as the catecholamines, can contribute to the ability of gram-negative pathogens to replicate in iron-restrictive media that may be reflective of gastrointestinal environments. the present report examines whether other, non-catechola ... | 2007 | 17850907 |
| salmonella sensing of anti-microbial mechanisms to promote survival within macrophages. | salmonella enterica is a facultative intracellular pathogen that replicates within macrophages. the interaction of this pathogen with mammalian cells is a complex process involving hundreds of bacterial products that are sensed by and alter mammalian hosts. numerous bacterial genes and their protein products have been identified that are required for salmonella to resist killing by host innate immunity and to modify host processes. many of these genes are regulated by a specific bacterial sensor ... | 2007 | 17850481 |
| microarray analysis of mu transposition in salmonella enterica, serovar typhimurium: transposon exclusion by high-density dna binding proteins. | all organisms contain transposons with the potential to disrupt and rearrange genes. despite the presence of these destabilizing sequences, some genomes show remarkable stability over evolutionary time. do bacteria defend the genome against disruption by transposons? phage mu replicates by transposition and virtually all genes are potential insertion targets. to test whether bacteria limit mu transposition to specific parts of the chromosome, dna arrays of salmonella enterica were used to quanti ... | 2007 | 17850262 |
| the role of cellulose and o-antigen capsule in the colonization of plants by salmonella enterica. | numerous salmonellosis outbreaks have been associated with vegetables, in particular sprouted seed. thin aggregative fimbriae (tafi), a component of the extracellular matrix responsible for multicellular behavior, are important for salmonella enterica attachment and colonization of plants. here, we demonstrate that the other surface polymers composing the extracellular matrix, cellulose, and o-antigen capsule also play a role in colonization of plants. mutations in bacterial cellulose synthesis ... | 2007 | 17849711 |
| phenotypic variations and molecular identification of salmonella enterica serovar typhimurium cells under starvation in seawater. | in seawater, enteric bacteria evolve toward a stressed state that is difficult to identify because of major alterations of their phenotype. in this study, we incubated four reference strains of s. enterica serovar typhimurium in seawater microcosms for 10 months and studied the modifications of their main phenotypic characters. all of the strains lost some key characters used for traditional identification of the salmonella genus. they became able to produce acetoin, and tryptophane deaminase ac ... | 2007 | 17828573 |
| lysine decarboxylase-negative salmonella enterica serovar enteritidis: antibiotic susceptibility, phage and pfge typing. | one hundred twenty salmonella enteritidis isolates collected from 1992 to 2005 in nagasaki prefecture (65 isolates from 40 outbreak cases, 44 from sporadic diarrhea patients, and 11 from chicken-related products) were investigated by their antibiotic susceptibility profiles, phage typing, and pulsed-field gel electrophoresis (pfge) typing. out of them, 18 were identified as lysine decarboxylase (ldc)-negative isolates, and 15 showed resistance toward streptomycin. based on the pfge typing, the i ... | 2007 | 17827887 |
| the cbib protein of salmonella enterica is an integral membrane protein involved in the last step of the de novo corrin ring biosynthetic pathway. | we report results of studies of the conversion of adenosylcobyric acid (adocby) to adenosylcobinamide-phosphate, the last step of the de novo corrin ring biosynthetic branch of the adenosylcobalamin (coenzyme b12) pathway of salmonella enterica serovar typhimurium lt2. previous reports have implicated the cbib protein in this step of the pathway. hydropathy analysis predicted that cbib would be an integral membrane protein. we used a computer-generated topology model of the primary sequence of c ... | 2007 | 17827296 |
| detailed structure of integrons and transposons carried by large conjugative plasmids responsible for multidrug resistance in diverse genomic types of salmonella enterica serovar brandenburg. | to evaluate the incidence, molecular basis and distribution among genomic types of antimicrobial drug resistance in salmonella enterica (s.) serovar brandenburg isolates recorded in the principality of asturias, spain. | 2007 | 17827139 |
| heterologous protection in pigs induced by a plasmid-cured and crp gene-deleted salmonella choleraesuis live vaccine. | in this study, we exploited a crp (camp receptor protein) gene-deleted, virulence plasmid-cured salmonella choleraesuis mutant with decreased carbon source utilization, designated s.c.-deltacrp/vpl(-), as a live vaccine strain. normal weight gain with no clinical signs was observed in pigs immunized with high doses of s.c.-deltacrp/vpl(-) live vaccine. vaccination in pregnant sows induced high maternal antibodies, which could prevent piglets from salmonella infection. moreover, serial transmissi ... | 2007 | 17825957 |
| the genome of epsilon15, a serotype-converting, group e1 salmonella enterica-specific bacteriophage. | the genome sequence of the salmonella enterica serovar anatum-specific, serotype-converting bacteriophage epsilon15 has been completed. the nonredundant genome contains 39,671 bp and 51 putative genes. it most closely resembles the genome of phiv10, an escherichia coli o157:h7-specific temperate phage, with which it shares 36 related genes. more distant relatives include the burkholderia cepacia-specific phage, bcepc6b (8 similar genes), the bordetella bronchiseptica-specific phage, bpp-1 (8 sim ... | 2007 | 17825342 |
| reinterpreting a community outbreak of salmonella enterica serotype enteritidis in the light of molecular typing. | in november 2005, a large outbreak due to salmonella enterica serotype enteritidis (s. enteritidis) was observed within children who had eaten their meals at 53 school cafeterias in florence and the surrounding area. a total of 154 isolates of s. enteritidis were recovered from human cases between november 2005 and january 2006. all strains were assigned phage type 8 (pt8) and a common xbai pulsotype. this paper reports the findings of a molecular epidemiological investigation performed on 124 s ... | 2007 | 17825103 |
| feasibility of a molecular screening method for detection of salmonella enterica and campylobacter jejuni in a routine community-based clinical microbiology laboratory. | conventional diagnostic methods for the detection of salmonella enterica and campylobacter jejuni are laborious and time-consuming procedures, resulting in final results, for the majority of specimens, only after 3 to 4 days. molecular detection can improve the time to reporting of the final results from several days to the next day. however, molecular assays for the detection of gastrointestinal pathogens directly from stool specimens have not made it into the routine clinical microbiology labo ... | 2007 | 17804656 |
| detection and identification of salmonella typhimurium in bovine diarrhoeic fecal samples by immunomagnetic separation and multiplex pcr assay. | the aim of this study was to use the immunomagnetic separation (ims) test plus a multiplex polymerase chain reaction (m-pcr) assay to detect salmonella at genus level and also for the identification of salmonella enterica serovar typhimurium in bovine diarrhoeic fecal samples. in all, 400 bovine diarrhoeic fecal specimens were examined by conventional bacterial culture, ims, and m-pcr. for m-pcr assay, four set primers were selected: 139-141, specific for inv-a gene of salmonella spp and the rfb ... | 2007 | 17803511 |
| variation in salmonella resistance to poultry chemical decontaminants, based on serotype, phage type, and antibiotic resistance patterns. | chemical decontaminants are currently under review for final approval by the european union authorities with the aim of reducing the number and/or prevalence of pathogenic microorganisms on poultry. the purpose of the research being reported here was to determine the association, if any, of decontaminant resistance with the serotype, phage type, and antibiotic resistance of salmonella strains. sixty poultry isolates of salmonella enterica (serotypes enteritidis: phage types 1, 4, 4b, 6a, 14b, an ... | 2007 | 17803139 |
| competitive inhibition bacteria of bovine origin against salmonella serovars. | studies were conducted to isolate bacteria inhibitory to salmonella enterica serovar typhimurium definitive type (dt) 104 in vitro from cattle not carrying salmonella and to determine the inhibitory activity of the isolated bacteria through competitive growth in cattle feces artificially contaminated with salmonella typhimurium dt104 and s. enterica serovar newport. fecal samples (108) were obtained from dairy and beef cows. s. enterica serovars were isolated from 9.25% of the samples and includ ... | 2007 | 17803135 |
| oral sodium chlorate, topical disinfection, and younger weaning age reduce salmonella enterica shedding in pigs. | salmonella enterica subsp. enterica can cause swine illness or human foodborne disease. although nontoxic to mammalian cells, chlorate can be converted to cytotoxic chlorite by salmonellae. to test whether chlorate is effective at reducing salmonella shedding in weaned pigs exposed to shedding dams, a chlorate-nitrate-lactate (chlorate) oral dose was administered daily for 5 days following weaning, and this treatment was evaluated in combination with two weaning ages and a topical disinfectant. ... | 2007 | 17803134 |
| splenic abscesses caused by a reptile-associated salmonella infection. | salmonella infections are not very uncommon. the source generally has to be looked for in food. the syndrome concerns mostly gastro-enteritis. we present a 17-year-old girl with sepsis caused by splenic abscesses which was successfully treated with splenectomy and antibiotics. after analysis (blood and surgical specimen samples), she appeared to be infected with salmonella type telelkebir, a rare variant that is associated with exotic animal species, mainly reptiles. the same variant was cultiva ... | 2007 | 17785987 |
| vertical transmission of salmonella paratyphi a. | neonatal enteric fever is a rare but life-threatening illness. patients may present with varying severity, salmonella enterica serotype typhi causing more severe illness than salmonella enterica serotype paratyphi a. salmonella enterica serotype paratyphi a is considered to cause milder infection with fewer complications. we report a rare case of vertical transmission of salmonella enterica serotype paratyphi a with severe complications and high mortality. even though there are case reports of v ... | 2007 | 17785907 |
| expression of the fis protein is sustained in late-exponential- and stationary-phase cultures of salmonella enterica serovar typhimurium grown in the absence of aeration. | the classic expression pattern of the fis global regulatory protein during batch culture consists of a high peak in the early logarithmic phase of growth, followed by a sharp decrease through mid-exponential growth phase until fis is almost undetectable at the end of the exponential phase. we discovered that this pattern is contingent on the growth regime. in salmonella enterica serovar typhimurium cultures grown in non-aerated spi1-inducing conditions, fis can be detected readily in stationary ... | 2007 | 17784910 |
| dissecting the salmonella response to copper. | intracellular copper homeostasis in bacteria is maintained as the result of a complex ensemble of cellular processes that in escherichia coli involve the coordinated action of two systems, cue and cus. in contrast, the pathogenic bacterium salmonella harbours only the cue regulon, including copa, which is shown here to be transcriptionally controlled by cuer. mutant strains in the cuer-regulated genes were constructed to characterize the response of salmonella enterica serovar typhimurium to hig ... | 2007 | 17768242 |
| the role of gene fusions in the evolution of metabolic pathways: the histidine biosynthesis case. | histidine biosynthesis is one of the best characterized anabolic pathways. there is a large body of genetic and biochemical information available, including operon structure, gene expression, and increasingly larger sequence databases. for over forty years this pathway has been the subject of extensive studies, mainly in escherichia coli and salmonella enterica, in both of which details of histidine biosynthesis appear to be identical. in these two enterobacteria the pathway is unbranched, inclu ... | 2007 | 17767732 |
| serodiagnosis of salmonella enterica serovar typhi and s. enterica serovars paratyphi a, b and c human infections. | the aim of this study was to evaluate an immunoassay for the detection of human serum antibodies to the lps and flagellar antigens of salmonella typhi and salmonella paratyphi a, b and c, and to the vi capsular polysaccharide of s. typhi and s. paratyphi c. a total of 330 sera were used; these originated from 15 patients who were culture-positive for s. typhi and 15 healthy controls, together with 300 sera submitted to the laboratory of enteric pathogens for salmonella serodiagnosis. by sds-page ... | 2007 | 17761477 |
| salmonella enterica serovar typhimurium exploits inflammation to compete with the intestinal microbiota. | most mucosal surfaces of the mammalian body are colonized by microbial communities ("microbiota"). a high density of commensal microbiota inhabits the intestine and shields from infection ("colonization resistance"). the virulence strategies allowing enteropathogenic bacteria to successfully compete with the microbiota and overcome colonization resistance are poorly understood. here, we investigated manipulation of the intestinal microbiota by the enteropathogenic bacterium salmonella enterica s ... | 2007 | 17760501 |
| salmonella: clinical importance and evolution of nomenclature. | salmonella is an important pathogen for both humans and animals. although the organism has been intensively studied during the last century, much remains to be learned about this pathogen. the complicated nomenclature system of salmonella has long been a subject of discussion. in 2005, "salmonella enterica" finally gained official approval as the type species of the genus salmonella. the genus salmonella also contains the species "salmonella bongori" in addition to a new species, "salmonella sub ... | 2007 | 17760271 |
| clonal expansion and microevolution of quinolone-resistant salmonella enterica serotype typhi in vietnam from 1996 to 2004. | salmonella enterica serotype typhi clinical isolates (n = 91) resistant to nalidixic acid (nal(r)) were collected from sporadic cases and minor outbreaks throughout vietnam between 1996 and 2004. these isolates were typed and compared by four methods: vi phage typing, psti ribotyping, xbai and spei pulsed-field gel electrophoresis (pfge), and single-nucleotide polymorphism (snp) analysis. the results indicated that 65% of the isolates were not typeable by vi phage typing. in contrast, the riboty ... | 2007 | 17728470 |
| single-nucleotide-polymorphism typing and genetic relationships of salmonella enterica serovar typhi isolates. | salmonella enterica serovar typhi is a clone with a low level of variation. we developed a molecular typing method for serovar typhi using 38 genome-wide single-nucleotide polymorphisms (snps) as markers detected by pcr-restriction enzyme digestion. the 73 worldwide serovar typhi isolates studied were separated into 23 snp profiles and four distinct genetic groups. serovar typhi isolates expressing the unique flagellar antigen z66 were found to cluster together and branch off from the ancestral ... | 2007 | 17728466 |
| pulmonary colonization with salmonella enterica serovar kentucky in an intensive care unit. | 2007 | 17728015 | |
| a new concept to stimulate mucosal as well as systemic immunity by parenteral vaccination as applied to the development of a live attenuated salmonella enterica serovar dublin vaccine. | a new concept of slow "drip feeding" that enables activation of mucosal as well as systemic immunity following parenteral vaccination was demonstrated using salmonella dublin in a mouse model. the live vaccine candidate, n-rm25, generated from a wild s. dublin strain utilising metabolic-drift (spontaneous chromosomal) mutations had a unique sensitivity to bile and restricted growth in the presence of a very low concentration of bile salts no. 3 (0.075% (w/v)) but also had the ability to survive ... | 2007 | 17727804 |
| a role for natural killer cells in intestinal inflammation caused by infection with salmonella enterica serovar typhimurium. | acute gastroenteritis caused by salmonella infection is a significant public health problem. using a mouse model of this condition, the authors demonstrated previously that the cytokine gamma interferon (ifn-gamma) is required for a normal intestinal inflammatory response to the pathogen. in the present study, these experiments are extended to show that natural killer (nk) cells constitute an early source of intestinal ifn-gamma during salmonella infection, and that these cells have a significan ... | 2007 | 17727655 |
| the salmonella enterica serotype typhi regulator tvia reduces interleukin-8 production in intestinal epithelial cells by repressing flagellin secretion. | unlike non-typhoidal salmonella serotypes, s. enterica serotype typhi does not elicit neutrophilic infiltrates in the human intestinal mucosa. the vi capsule-encoding tviabcdevexabcde operon (viab locus) is a s. typhi-specific dna region preventing production of interleukin (il)-8 during infection of intestinal epithelial cells. we elucidated the mechanism by which the viab locus reduces il-8 production in human colonic epithelial (t84) cells. a s. typhi tviabcdevexabcde deletion mutant, but not ... | 2008 | 17725646 |
| evaluation of phop and rpos mutants of salmonella enterica serovar typhi as attenuated typhoid vaccine candidates: virulence and protective immune responses in intranasally immunized mice. | the attenuation and immunoenhancing effects of rpos and phop salmonella enterica serovar strain typhi (salmonella typhi) mutants have not been compared. here, three s. typhi deletion mutants (phop, rpos, and rpos-phop double mutant) are constructed and these mutants are characterized with respect to invasiveness, virulence, and protective immune response compared with wild-type ty2. it was found that phop and phop-rpos deletion mutants are less invasive to ht-29 cells than the wild-type ty2 and ... | 2007 | 17725620 |
| systemic translocation of salmonella enterica serovar dublin in cattle occurs predominantly via efferent lymphatics in a cell-free niche and requires type iii secretion system 1 (t3ss-1) but not t3ss-2. | salmonella enterica is an important diarrheal pathogen, and infections may involve severe systemic sequelae depending on serovar- and host-specific factors. the molecular mechanisms underlying translocation of host-restricted and -specific serovars of s. enterica from the intestines to distal organs are ill defined. by surgical cannulation of lymph and blood vessels draining the distal ileum in cattle, s. enterica serovar dublin was observed to translocate predominantly via mesenteric lymph node ... | 2007 | 17724072 |
| cobalt targets multiple metabolic processes in salmonella enterica. | cobalt is essential for growth of salmonella enterica and other organisms, yet this metal can be toxic when present in excess. wild-type salmonella exhibits several metabolic defects when grown in the presence of cobalt, some of which generate visible growth consequences. work herein identifies sulfur assimilation, iron homeostasis, and fe-s cluster metabolism as targets for cobalt toxicity. in each case it is proposed that cobalt exerts its effect by one of two mechanisms: direct competition wi ... | 2007 | 17720790 |
| spontaneous zygogenesis, a wide-ranging mating process in bacteria. | spontaneous zygogenesis (or z-mating) in escherichia coli, unlike f-mediated conjugation, promotes formation of complete zygotes. most z-mating products have proven to be phenotypically unstable, losing part of the phenotype for which they were selected. persistent z-mating products appear as stable genetic recombinants or yield subclones of two types: either both parental types or one of them plus a recombinant type, and the ability to promote spontaneous zygogenesis can appear in all emerging ... | 2007 | 17720456 |
| monocyte and dendritic cell recruitment and activation during oral salmonella infection. | immunity to bacterial infection involves the joint effort of the innate and adaptive immune systems. the innate immune response is triggered when the body senses bacterial components, such as lipopolysaccharide, that alarm the body of the invader. an array of cell types function in the innate response. these cells are rapidly recruited to the infection site and activated to optimally perform their functions. the adaptive immune response follows the innate response, and one cell type in particula ... | 2007 | 17720254 |
| protective action of lactobacillus kefir carrying s-layer protein against salmonella enterica serovar enteritidis. | eight lactobacillus kefir strains isolated from different kefir grains were tested for their ability to antagonize salmonella enterica serovar enteritidis (salmonella enteritidis) interaction with epithelial cells. l. kefir surface properties such as autoaggregation and coaggregation with salmonella and adhesion to caco-2/tc-7 cells were evaluated. l. kefir strains showed significantly different adhesion capacities, six strains were able to autoaggregate and four strains coaggregated with salmon ... | 2007 | 17719671 |
| bringing order to a complex molecular machine: the assembly of the bacterial flagella. | the bacterial flagellum is an example of elegance in molecular engineering. flagella dependent motility is a widespread and evolutionarily ancient trait. diverse bacterial species have evolved unique structural adaptations enabling them to migrate in their environmental niche. variability exists in the number, location and configuration of flagella, and reflects unique adaptations of the microorganism. the most detailed analysis of flagellar morphogenesis and structure has focused on escherichia ... | 2008 | 17719558 |
| the pattern of pleiomorphism in stressed salmonella virchow populations is nutrient and growth phase dependent. | to describe the interactions of imposed osmotic and nutritional stress on the morphology of stationary and exponential phase s. virchow cells. | 2007 | 17718839 |
| temporal fragmentation of speciation in bacteria. | because bacterial recombination involves the occasional transfer of small dna fragments between strains, different sets of niche-specific genes may be maintained in populations that freely recombine at other loci. therefore, genetic isolation may be established at different times for different chromosomal regions during speciation as recombination at niche-specific genes is curtailed. to test this model, we separated sequence divergence into rate and time components, revealing that different reg ... | 2007 | 17717188 |
| one step engineering of t7-expression strains for protein production: increasing the host-range of the t7-expression system. | the t7-expression system has been very useful for protein expression in escherichia coli. however, it is often desirable to over-express proteins in species other than e. coli. here, we constructed an inducible broad-host-range t7-expression transposon, which allows simple one-step construction of t7-expression strains in various species, providing the option to over-express proteins of interest in a broader host-range. this transposon contains the t7 rna polymerase driven by the lacuv5 promoter ... | 2007 | 17716915 |
| salmonella pathogenicity islands in host specificity, host pathogen-interactions and antibiotics resistance of salmonella enterica. | salmonella enterica is a pathogen highly successful in causing a variety of gastrointestinal and systemic diseases in animals and humans. while some serovars of s. enterica are able to infect a broad range of host organisms, other serovars are highly restricted to specific host species. the colonization of hosts by s. enterica depends on the function of a large number of virulence determinants. the molecular analyses of virulence genes demonstrated that most of these loci are clustered within sa ... | 2007 | 17715824 |
| it's not easy being green. | 2007 | 17714671 | |
| pyroptosis and host cell death responses during salmonella infection. | salmonella enterica are facultatively intracellular pathogens causing diseases with markedly visible signs of inflammation. during infection, salmonella interacts with various host cell types, often resulting in death of those cells. salmonella induces intestinal epithelial cell death via apoptosis, a cell death programme with a notably non-inflammatory outcome. in contrast, macrophage infection triggers caspase-1-dependent proinflammatory programmed cell death, a recently recognized process ter ... | 2007 | 17714514 |
| identification and characterization of starter lactic acid bacteria and probiotics from columbian dairy products. | considering the significant rise in the probiotic market in columbia, and given the lack of reports concerning the microbial population and strain performance in products from different producers, this study aims at determining the number of viable starter bacteria and probiotics in bio-yoghurts available at the columbian market, identifying the species and analysing the performance of the isolated strains in bile acid resistance, antagonistic activity against pathogens, and adherence capacity t ... | 2007 | 17714400 |
| the lactobacillus plantarum strain aca-dc287 isolated from a greek cheese demonstrates antagonistic activity in vitro and in vivo against salmonella enterica serovar typhimurium. | the purpose of this study was to investigate the antibacterial activity of the xynotyri cheese isolate lactobacillus plantarum aca-dc287 using a set of in vitro and in vivo assays. | 2007 | 17714399 |
| growth inhibitory factors in bovine faeces impairs detection of salmonella dublin by conventional culture procedure. | to analyse the relative importance of different biological and technical factors on the analytical sensitivity of conventional culture methods for detection of salmonella dublin in cattle faeces. | 2007 | 17714398 |
| multiple-locus variable-number tandem repeat analysis of salmonella enterica subsp. enterica serovar typhimurium: comparison of isolates from pigs, poultry and cases of human gastroenteritis. | pulsed-field gel electrophoresis (pfge) and variable number tandem repeat (vntr) profiles of 195 epidemiologically unrelated salmonella typhimurium strains isolated in 1997-2004 from pigs were analysed and the results compared to establish the discriminatory ability of each method. in order to investigate the epidemiology of s. typhimurium from different populations, the vntr profiles from pigs were compared with those obtained from 190 s. typhimurium strains isolated from poultry and 186 strain ... | 2007 | 17714389 |
| predictive models for reduction of salmonella hadar on chicken skin during single and double sequential spraying treatments with acetic acid. | the response surface methodology was used to evaluate the effect of acetic acid concentration, spraying time and temperature on the reduction of salmonella hadar on poultry skin in a laboratory spraying process, and to identify the best conditions required to develop this operation. | 2007 | 17714385 |
| late recurrent salmonella sacroiliac osteomyelitis with psoas abscess in a non-sickle cell adult: case report. | 2007 | 17712436 | |
| contribution of spi-4 genes to the virulence of salmonella enterica. | salmonella pathogenicity island-4 (spi-4) is a 27-kb region that carries six genes designated siiabcdef. siic, siid, and siif form a type i secretion apparatus for the secretion of siie, a huge (approximately 600 kda) protein contributing to the colonization of the bovine intestines. here it is shown that loss of spi-4 attenuates the oral virulence of salmonella enterica serovars typhimurium and enteritidis in mice. fifty percent lethal doses were elevated in both serovars upon the loss of spi-4 ... | 2007 | 17711458 |
| omptin proteins: an expanding family of outer membrane proteases in gram-negative enterobacteriaceae. | the escherichia coli k-12 outer membrane protein ompt is a prototype of a unique family of bacterial endopeptidases known as the omptins. this family includes ompt and ompp of e. coli, sopa of shigella flexneri, pgte of salmonella enterica, and pla of yersinia pestis. despite their sequence similarities, the omptins vary in their reported functions. the ompt protease is characterized by narrow cleavage specificity defined by the extracellular loops of the beta-barrel protruding above the lipid b ... | 2007 | 17710644 |
| contribution of the stg fimbrial operon of salmonella enterica serovar typhi during interaction with human cells. | salmonella serovars contain a wide variety of putative fimbrial systems that may contribute to colonization of specific niches. salmonella enterica serovar typhi is the etiologic agent of typhoid fever and is a pathogen specific to humans. in a previous study, we identified a gene, sty3920 (stgc), encoding the predicted usher of the stg fimbrial operon, that was expressed by serovar typhi during infection of human macrophages. the stg genes are located in the glms-psts intergenic region in serov ... | 2007 | 17709421 |
| chicken cecum immune response to salmonella enterica serovars of different levels of invasiveness. | day-old chicks are very susceptible to infections with salmonella enterica subspecies. the gut mucosa is the initial site of host invasion and provides the first line of defense against the bacteria. to study the potential of different s. enterica serovars to invade the gut mucosa and trigger an immune response, day-old chicks were infected orally with salmonella enterica serovar enteritidis, s. enterica serovar typhimurium, s. enterica serovar hadar, or s. enterica serovar infantis, respectivel ... | 2007 | 17709416 |
| trends in salmonella enterica serotypes isolated from human, food, animal, and environment in tunisia, 1994-2004. | the present study was conducted to investigate, for the first time in tunisia, the current trends in salmonella enterica serotypes in tunisia (human, food, animal, and environment) which would help to improve the control and prevention of salmonella infections. | 2007 | 17706289 |
| ability of bacteriophages isolated from different sources to reduce salmonella enterica serovar enteritidis in vitro and in vivo. | salmonella enterica serovar enteritidis-lysing bacteriophages isolated from poultry or human sewage sources were used to reduce salmonella enteritidis in vitro and in experimentally infected chicks. cocktails of 4 different bacteriophages obtained from commercial broiler houses (cb4ø) and 45 bacteriophages from a municipal wastewater treatment plant (wt45ø) were evaluated. in experiment 1, an in vitro crop assay was conducted with selected bacteriophage concentrations (10(5) to 10(9) pfu/ml) to ... | 2007 | 17704377 |
| influence of lactic acid bacteria on longevity of caenorhabditis elegans and host defense against salmonella enterica serovar enteritidis. | this study aimed to develop a convenient model to investigate the senescence of host defenses and the influence of food and nutrition. a small soil nematode, caenorhabditis elegans, was grown for 3 days from hatching on a lawn of escherichia coli op50 as the normal food source, and subsequently some of the nematodes were fed lactic acid bacteria (lab). the life spans of worms fed lab were significantly longer than the life spans of those fed op50. to investigate the effect of age on host defense ... | 2007 | 17704266 |
| induction of rpos degradation by the two-component system regulator rsta in salmonella enterica. | bacterial survival in diverse and changing environments relies on the accurate interplay between different regulatory pathways, which determine the design of an adequate adaptive response. the proper outcome depends on a precise gene expression profile generated from the finely tuned and concerted action of transcriptional factors of distinct regulatory hierarchies. salmonella enterica serovar typhimurium harbors multiple regulatory systems that are crucial for the bacterium to cope with harsh e ... | 2007 | 17704217 |
| improving live attenuated bacterial carriers for vaccination and therapy. | live attenuated bacteria are well established as vaccines. thus, their use as carriers for prophylactic and therapeutic macromolecules is a logical consequence. here we describe several experimental applications of bacteria to carry heterologous macromolecules into the murine host. first, listeria monocytogenes are described that are able to transfer eukaryotic expression plasmids into host cells for gene therapy. high multiplicities of infection are still required for efficient gene transfer an ... | 2008 | 17702649 |
| comparative analysis of inchi2 plasmids carrying blactx-m-2 or blactx-m-9 from escherichia coli and salmonella enterica strains isolated from poultry and humans. | salmonella enterica bla(ctx-m-2) and bla(ctx-m-9) plasmid backbones from isolates from belgium and france were analyzed. the bla(ctx-m-2-)plasmids from both human and poultry isolates were related to the inchi2 papec-o1-r plasmid, previously identified in the united states in avian escherichia coli strains; the bla(ctx-m-9) plasmids were closely related to the inchi2 r478 plasmid. | 2007 | 17698627 |
| constitutive acid sphingomyelinase enhances early and late macrophage killing of salmonella enterica serovar typhimurium. | we have identified acid sphingomyelinase (asm) as an important player in the early and late anti-salmonella activity of macrophages. a functional asm participated in the killing activity of macrophages against wild-type salmonella enterica serovar typhimurium. the role of asm in early macrophage killing of salmonella appears to be linked to an active nadph phagocyte oxidase enzymatic complex, since the flavoprotein inhibitor diphenyleneiodonium not only blocked a productive respiratory burst but ... | 2007 | 17698574 |
| lipopolysaccharide from salmonella enterica activates nf-kappab through both classical and alternative pathways in primary b lymphocytes. | lipopolysaccharides (lps) are potent polyclonal b-lymphocyte activators. recently, we have shown that lps inhibits both spontaneous and drug-induced apoptosis in mature b lymphocytes, through cytosolic retention of bax, a proapoptotic protein of the bcl-2 family, by preventing its translocation to mitochondria. research within the last few years has revealed that members of the nf-kappab transcription factor regulate cell viability by activating genes involved in mitochondrion-dependent apoptosi ... | 2007 | 17698569 |
| regional ifngamma expression is insufficient for efficacious control of food-borne bacterial pathogens at the gut epithelial barrier. | ifngamma is critical for host defence against various food-borne pathogens including salmonella enterica and listeria monocytogenes, the causative agents of salmonellosis and listeriosis, respectively. we investigated the impact of regional ifngamma expression at the intestinal epithelial barrier on host invasion by salmonellae and listeriae following oral challenge. transgenic mice (ifngamma-gut), generated on an ifngamma knock-out (ko) background, selectively expressed ifngamma in the gut driv ... | 2007 | 17698562 |
| identification of the salmonella enterica serotype typhimurium sipa domain responsible for inducing neutrophil recruitment across the intestinal epithelium. | in human intestinal disease induced by salmonella enterica serotype typhimurium (s. typhimurium) transepithelial migration of polymorphonuclear leukocytes (pmns) rapidly follows attachment of the bacteria to the epithelial apical membrane. previously, we have shown that the s. typhimurium effector protein, sipa, plays a pivotal role in signalling epithelial cell responses that lead to the transepithelial migration of pmns. thus, the objective of this study was to determine the functional domain ... | 2007 | 17697195 |
| molecular characterisation of multidrug-resistant salmonella enterica serovar typhimurium isolates from gomel region, belarus. | this study describes the characterisation by pulsed-field gel electrophoresis (pfge), multilocus variable number tandem repeat analysis (mlva) typing and antimicrobial resistance profiles of 35 salmonella enterica serovar typhimurium isolates, mostly from infections in children who acquired an infection outside hospitals in the gomel region of belarus. thirty-one isolates were highly similar according to pfge and mlva typing, were multidrug-resistant, including resistance to ceftiofur, and harbo ... | 2007 | 17697002 |
| sopd acts cooperatively with sopb during salmonella enterica serovar typhimurium invasion. | the intracellular bacterial pathogen, salmonella enterica serovar typhimurium (s. typhimurium), causes disease in a variety of hosts. to invade and replicate in host cells, these bacteria subvert host molecular machinery using bacterial proteins, called effectors, which they translocate into host cells using specialized protein delivery systems. one of these effectors, sopd, contributes to gastroenteritis, systemic virulence and persistence of s. typhimurium in animal models of infection. recent ... | 2007 | 17696999 |
| molecular characterization of the thi3 gene involved in thiamine biosynthesis in zea mays: cdna sequence and enzymatic and structural properties of the recombinant bifunctional protein with 4-amino-5-hydroxymethyl-2-methylpyrimidine (phosphate) kinase and thiamine monophosphate synthase activities. | a thiamine biosynthesis gene, thi3, from maize zea mays has been identified through cloning and sequencing of cdna and heterologous overexpression of the encoded protein, thi3, in escherichia coli. the recombinant thi3 protein was purified to homogeneity and shown to possess two essentially different enzymatic activities of hmp(-p) [4-amino-5-hydroxymethyl-2-methylpyrimidine (phosphate)] kinase and tmp (thiamine monophosphate) synthase. both activities were characterized in terms of basic kineti ... | 2007 | 17696876 |
| recombining population structure of plesiomonas shigelloides (enterobacteriaceae) revealed by multilocus sequence typing. | plesiomonas shigelloides is an emerging pathogen that is widespread in the aquatic environment and is responsible for intestinal diseases and extraintestinal infections in humans and other animals. virtually nothing is known about its genetic diversity, population structure, and evolution, which severely limits epidemiological control. we addressed these questions by developing a multilocus sequence typing (mlst) system based on five genes (fusa, leus, pyrg, recg, and rpob) and analyzing 77 epid ... | 2007 | 17693512 |
| phopq-mediated regulation produces a more robust permeability barrier in the outer membrane of salmonella enterica serovar typhimurium. | the phopq two-component system of salmonella enterica serovar typhimurium produces a remodeling of the lipid a domain of the lipopolysaccharide, including the pagp-catalyzed addition of palmitoyl residue, the pmrab-regulated addition of the cationic sugar 4-aminoarabinose and phosphoethanolamine, and the lpxo-catalyzed addition of a 2-oh group onto one of the fatty acids. by using the diffusion rates of the dyes ethidium, nile red, and eosin y across the outer membrane, as well as the susceptibi ... | 2007 | 17693506 |
| the thiamine kinase (ycfn) enzyme plays a minor but significant role in cobinamide salvaging in salmonella enterica. | cobinamide (cbi) salvaging is impaired, but not abolished, in a salmonella enterica strain lacking a functional cobu gene. cobu is a bifunctional enzyme (ntp:adenosylcobinamide [ntp:adocbi] kinase, gtp:adenosylcobinamide-phosphate [gtp:adocbi-p] guanylyltransferase) whose adocbi kinase activity is necessary for cbi salvaging in this bacterium. inactivation of the ycfn gene in a deltacobu strain abrogated cbi salvaging. introduction of a plasmid carrying the ycfn(+) allele into a deltacobu deltay ... | 2007 | 17693493 |
| concurrent infections in acute febrile illness patients in egypt. | we report the occurrence of concurrent infections with multiple acute febrile illness (afi) pathogens during an ongoing prospective laboratory-based surveillance in four infectious disease hospitals in urban and rural areas of egypt from june 2005 to august 2006. patients were screened for leptospira, rickettsia typhi, brucella, or salmonella enterica serogroup typhi by various methods including serology, culture, and pcr. one hundred eighty-seven of 1,510 patients (12.4%) evaluated had supporti ... | 2007 | 17690420 |
| salmonella enterica serovar typhi ty21a expressing human papillomavirus type 16 l1 as a potential live vaccine against cervical cancer and typhoid fever. | human papillomavirus (hpv) vaccines based on l1 virus-like particles (vlps) can prevent hpv-induced genital neoplasias, the precursors of cervical cancer. however, most cervical cancers occur in developing countries, where the implementation of expensive vaccines requiring multiple injections will be difficult. a live salmonella-based vaccine could be a lower-cost alternative. we previously demonstrated that high hpv type 16 (hpv16)-neutralizing titers are induced after a single oral immunizatio ... | 2007 | 17687110 |
| glutamine phosphoribosylpyrophosphate amidotransferase-independent phosphoribosyl amine synthesis from ribose 5-phosphate and glutamine or asparagine. | phosphoribosylamine (pra) is the first intermediate in the common pathway to purines and thiamine and is generated in bacteria by glutamine phosphoribosylpyrophosphate (prpp) amidotransferase (ec 2.4.2.14) from prpp and glutamine. genetic data have indicated that multiple, non-prpp amidotransferase mechanisms exist to generate pra sufficient for thiamine but not purine synthesis. here we describe the purification and identification of an activity (present in both escherichia coli and salmonella ... | 2007 | 17686772 |
| part i. analysis of data gaps pertaining to salmonella enterica serotype typhi infections in low and medium human development index countries, 1984-2005. | there are only 10 contemporary, population-based studies of typhoid fever that evaluate disease incidence using blood culture for confirmation of cases. reported incidence ranged from 13 to 976/100 000 persons per year. these studies are likely to have been done preferentially in high- incidence sites which makes generalization of data difficult. only five of these studies reported mortality. of these the median (range) mortality was 0% (0-1.8%). since study conditions usually involved enhanced ... | 2008 | 17686194 |
| protective role of hemidesmus indicus r. br. root extract against salmonella typhimurium-induced cytotoxicity in int 407 cell line. | the present investigation deals with the effect of the chloroform fraction composed of sterols and fatty acids isolated from hemidesmus indicus root extract (chi) on salmonella enterica serovar typhimurium (s. typhimurium)-induced cytotoxicity in a human intestinal epithelial cell line (int 407). the optimum dose was fixed as 100 microg/ml for chi against s. typhimurium, which was quite safe for int 407 cells as the cd(50) concentration (50% cell death) of chi was determined to be 500 microg/ml ... | 2007 | 17685386 |
| outbreaks where food workers have been implicated in the spread of foodborne disease. part 1. description of the problem, methods, and agents involved. | food workers in many settings have been responsible for foodborne disease outbreaks for decades, and there is no indication that this is diminishing. the committee on control of foodborne illnesses of the international association for food protection was tasked with collecting and evaluating any data on worker-associated outbreaks. a total of 816 reports with 80,682 cases were collected from events that occurred from 1927 until the first quarter of 2006. most of the outbreaks reviewed were from ... | 2007 | 17685355 |
| assessment of contamination potential of lettuce by salmonella enterica serovar newport added to the plant growing medium. | the capacity of salmonella enterica serovar newport to contaminate romaine lettuce (lactuca sativa l. cv. nogal) via the root system was evaluated in 17-, 20-, and 33-day-old plants. apparent internalization of salmonella via the root to the above-ground parts was identified in 33- but not 17- or 20-day-old plants and was stimulated by root decapitation. leaves of lettuce plants with intact and damaged roots harbored salmonella at 500 +/- 120 and 5,130 +/- 440 cfu/g of leaf, respectively, at 2 d ... | 2007 | 17685348 |
| n-lysine propionylation controls the activity of propionyl-coa synthetase. | reversible protein acetylation is a ubiquitous means for the rapid control of diverse cellular processes. acetyltransferase enzymes transfer the acetyl group from acetyl-coa to lysine residues, while deacetylase enzymes catalyze removal of the acetyl group by hydrolysis or by an nad(+)-dependent reaction. propionyl-coenzyme a (coa), like acetyl-coa, is a high energy product of fatty acid metabolism and is produced through a similar chemical reaction. because acetyl-coa is the donor molecule for ... | 2007 | 17684016 |
| cytokine gene expression in chicken cecal tonsils following treatment with probiotics and salmonella infection. | probiotics are currently employed for control of pathogens and enhancement of immune response in chickens. in this study, we investigated the underlying immunological mechanisms of the action of probiotics against colonization of the chicken intestine by salmonella enterica subsp. enterica serovar typhimurium (salmonella serovar typhimurium). birds received probiotics by oral gavage on day 1 of age and, subsequently, received salmonella serovar typhimurium on day 2 of age. cecal tonsils were rem ... | 2008 | 17681719 |
| immune complex-induced enhancement of bacterial antigen presentation requires fcgamma receptor iii expression on dendritic cells. | dendritic cells (dcs) are capable of initiating adaptive immune responses against infectious agents by presenting pathogen-derived antigens on mhc molecules to naïve t cells. because of their key role in priming adaptive immunity, it is expected that interfering with dc function would be advantageous to the pathogen. we have previously shown that salmonella enterica serovar typhimurium (st), is able to survive inside dcs and interfere with their function by avoiding activation of bacteria-specif ... | 2007 | 17679697 |
| susceptibility of germ-free pigs to challenge with protease mutants of salmonella enterica serovar typhimurium. | salmonella protease mutants, clpp and especially htra, are candidate live oral vaccines in humans. a functional and mature immune system is, however, required to cope with them in mice. here, we test the cytokine response of highly susceptible germ-free pigs to infection with salmonella typhimurium clpp and htra mutants. cytokine levels (il-4, il-10, il-18 and ifn-gamma) were measured by elisa in plasma and washes from the terminal small bowel 24h after oral challenge. unlike the infection with ... | 2007 | 17678715 |
| global gene expression profile after salmonella enterica serovar enteritidis challenge in two f8 advanced intercross chicken lines. | a chicken 13k cdna microarray was used to profile global gene expression after salmonella enteritidis (se) challenge of young chickens. two f8 advanced intercross lines (ail), broiler by leghorn, and broiler by fayoumi, were studied. day-old chicks were orally inoculated with se, and spleens were harvested at day 7 or 8 post-inoculation. the se bacteria burden in the spleen was quantified. the 20% high and 20% low se burden birds within each ail and harvest time were studied by microarray. the l ... | 2007 | 17675853 |
| icsbp1/irf-8 is required for innate and adaptive immune responses against intracellular pathogens. | the chronic myeloid leukemia syndrome of the bxh-2 mouse strain (mus musculus) is caused by a recessive mutation (r294c) in the transcriptional regulator icsbp1/irf-8. in trans activation assays using an il-12p40 gene reporter construct introduced in raw 264.7 mouse macrophages, we show that the icsbp1(c294) isoform behaves as a partial loss-of-function. the icsbp1(c294) hypomorph allele appears to have a threshold effect on il-12 production, with pleiotropic consequences on resistance to differ ... | 2007 | 17675508 |
| cloning and transfer of the salmonella pathogenicity island 2 type iii secretion system for studies of a range of gram-negative genera. | the engineering of bacterial strains with specific phenotypes frequently requires the use of blocks or "cassettes" of genes that act together to perform a desired function. the potential benefits of utilizing type iii secretion systems in this regard are becoming increasingly realized since these systems can be used to direct interactions with host cells for beneficial purposes such as vaccine development, anticancer therapies, and targeted protein delivery. however, convenient methods to clone ... | 2007 | 17675443 |
| isolation of salmonella enterica serovar enteritidis from houseflies (musca domestica) found in rooms containing salmonella serovar enteritidis-challenged hens. | houseflies (musca domestica) released into rooms containing hens challenged with salmonella enterica serovar enteritidis (salmonella serovar enteritidis) rapidly became contaminated with salmonella serovar enteritidis. forty to 50% of the flies were contaminated at 48 h, and the percentage increased to 50 to 70% at 4 and 7 days postexposure and then decreased to 30% at day 15. initial attempts at recovering surface organisms for culture using an aqueous rinse were largely unsuccessful, while cul ... | 2007 | 17675422 |
| role of nucleoid-associated proteins hha and h-ns in expression of salmonella enterica activators hild, hilc, and rtsa required for cell invasion. | the coordinate expression of salmonella enterica invasion genes on salmonella pathogenicity island 1 is under the control of the complex circuits of regulation that involve the arac/xyls family transcriptional activators hild, hilc, and rtsa and nucleoid-associated proteins. single-copy transcription fusions were used to assess the effects of nucleoid-associated proteins hha and h-ns on hild, hilc, and rtsa expression. the data show that all three genes, hild, hilc, and rtsa, were repressed by h ... | 2007 | 17675384 |
| a proteomic approach to study salmonella enterica serovar typhimurium putative transporter yjeh associated with ceftriaxone resistance. | mutant 6b7 of salmonella enterica serovar typhimurium has a transposon inserted in the putative transporter gene yjeh and shows a more-than-fourfold reduction in resistance to ceftriaxone. in this report we have used proteomic analysis to compare outer membrane protein profiles between this mutant and its parental strain r200. five identified proteins were found to be altered. of these proteins, the level of expression of the porin ompd was increased and those of the putative outer membrane prot ... | 2007 | 17669360 |
| i/st mice hypersusceptible to mycobacterium tuberculosis are resistant to m. avium. | we previously demonstrated that mice of the i/st strain are extremely susceptible to mycobacterium tuberculosis, as well as to the taxonomically distant intracellular bacteria chlamydia pneumoniae and salmonella enterica. to broaden our knowledge about the control of susceptibility to intracellular pathogens, we studied the infection caused by mycobacterium avium virulent strain 724 in a panel of inbred mouse strains and found that i/st mice are resistant to m. avium. by comparing i/st mice with ... | 2007 | 17664269 |
| shigella flexneri phagosomal escape is independent of invasion. | infections with salmonella enterica serovar typhimurium and shigella flexneri result in mucosal inflammation in response to epithelial cell invasion and macrophage cytotoxicity. these processes are mediated by type iii secretion systems encoded in homologous virulence loci in the two species, namely, salmonella pathogenicity island 1 (spi-1), carried in the genome, and the shigella entry region (ser), carried in a large virulence plasmid. here we show that spi-1 can functionally complement a del ... | 2007 | 17664266 |
| the escherichia coli arac-family regulators gadx and gadw activate gade, the central activator of glutamate-dependent acid resistance. | escherichia coli can survive ph 2 acid stress by using several acid resistance systems. the most efficient of these employs glutamate decarboxylase (gada/gadb) to consume protons, and an antiporter (gadc) to exchange the intracellular decarboxylation product for external glutamic acid. expression of the essential transcriptional activator of this system, gade, is controlled by several regulators in a hierarchical fashion. in this study, two additional activators have been identified. the arac-fa ... | 2007 | 17660422 |
| mutations in yhit enable utilization of exogenous pyrimidine intermediates in salmonella enterica serovar typhimurium. | mutants capable of utilizing the pyrimidine biosynthetic intermediates carbamoylaspartate and dihydroorotate for growth were derived from pyrimidine auxotrophs of salmonella enterica serovar typhimurium lt2. the gain-of-function phenotypes both resulted from mutations in a single gene, yhit, the third gene of a putative four-gene operon, yhivuts, for which there is no homologous region in escherichia coli. notably, when a mutant yhit allele was transferred to a pyrimidine-requiring e. coli strai ... | 2007 | 17660412 |
| alterations in the two globular domains or in the connecting alpha-helix of bacterial ribosomal protein l9 induces +1 frameshifts. | the ribosomal 50s subunit protein l9, encoded by the gene rpli, is an elongated protein with an alpha-helix connecting the n- and c-terminal globular domains. we isolated rpli mutants that suppress the +1 frameshift mutation hisc3072 in salmonella enterica serovar typhimurium. these mutants have amino acid substitutions in the n-terminal domain (g24d) or in the c-terminal domain (i94s, a102d, g126v, and f132s) of l9. in addition, different one-base deletions in rpli altered either the final port ... | 2007 | 17660285 |
| characterization of the osmoprotectant transporter opuc from pseudomonas syringae and demonstration that cystathionine-beta-synthase domains are required for its osmoregulatory function. | the plant pathogen pseudomonas syringae may cope with osmotic stress on plants, in part, by importing osmoprotective compounds. in this study, we found that p. syringae pv. tomato strain dc3000 was distinct from most bacterial species in deriving greater osmoprotection from exogenous choline than from glycine betaine. this superior osmoprotection was correlated with a higher capacity for uptake of choline than for uptake of glycine betaine. of four putative osmoregulatory abc transporters in dc3 ... | 2007 | 17660277 |
| geobacillus stearothermophilus lv cada gene mediates resistance to cadmium, lead and zinc in znta mutants of salmonella entérica serovar typhimurium. | salmonella entérica serovar typhimurium cells expressing the cada gene of geobacillus stearothermophilus lv exhibit a hypersensitive phenotype to cadmium chloride. deletion of the orf stm3576 from the salmonella genome resulted in cadmium, lead and zinc sensitivity, confirming that this orf is a homologue of the znta gene. the observed sensitivity was reverted upon expression of the g. stearothermophilus lv cada gene. these results indicate that the cada gene product is involved in cd, pb and zn ... | 2007 | 17657347 |
| complete nucleotide sequence of a small qnrs1-carrying plasmid from salmonella enterica subsp. enterica typhimurium dt193. | 2007 | 17652106 | |
| prevalence, antimicrobial resistance, and extended-spectrum beta-lactamases characterization of salmonella isolates in apulia, southern italy (2001-2005). | the prevalence, antimicrobial susceptibility, and production of extended-spectrum beta-lactamases (esbls) of salmonella collected from several hospitals in apulia (southern italy) were evaluated. the most common salmonella isolates were salmonella enterica serovar typhimurium (44.6%), s. enterica serovar enteritidis (33.4 %), s. enterica serovar infantis (3.2 %), s. enterica serovar typhi (1.5%), and s. enterica serovar bovismorbificans (1.5%). the other serovars accounted for less than 1% each. ... | 2007 | 17650965 |