Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| immunoelectrophoretic identification and purification of herpes simplex virus antigens released from infected cells in tissue culture. | proteins released from herpes simplex virus type 1 (hsv-1)-and type 2 (hsv-2)-infected hep-2 cells have been characterized by the crossed immunoelectrophoretic technique. both hsv type-common and type-specific antigens were found in the tissue culture medium 24 h after infection. antigen ag-6, an hsv-1-specific antigen, was found in high concentration in the medium as compared to other hsv antigens released from hsv-1-infected cells. the hsv-2-specific antigens, ag-4 and ag-1, were released in m ... | 1979 | 85610 |
| type-common cp-1 antigen of herpes simplex virus is associated with a 59,000-molecular-weight envelope glycoprotein. | the cp-1 antigen of herpes simplex virus type 1 (hsv-1) is a glycoprotein found in the soluble portion of infected cells, in detergent extracts of infected cell membranes, and in the envelope of purified virus. antisera were prepared against a further purified form of cp-1 prepared from hsv soluble antigen mix; a glycoprotein, gp52, isolated from detergent-treated infected cells; and detergent extracts of purified virus. each of the antisera reacted with cp-1 to give a single immunoprecipitin ba ... | 1978 | 80458 |
| antibodies to herpes simplex virus type 2 in husbands of patients with cervical carcinoma. | 1978 | 79964 | |
| identification of a common antigen of herpes simplex virus bovine herpes mammillitis virus, and b virus. | in immunoelectrophoretic analyses one common antigen was demonstrated in antigen preparations from herpes simplex virus types 1- and 2- (hsv-1 and hsv-2), bovine herpes mammillitis (bhm) virus-, and b virus-infected cells solubilized by triton x-100. the antigen was also demonstrated in solubilized purified hsv-1 and bhm virus. the common antigen was identified as antigen 11 of hsv-1 or hsv-2. differences were found in the polypeptide composition of the related antigens when isolated from the fo ... | 1978 | 78988 |
| elisa for herpes simplex virus type 2 antibodies. | 1977 | 72945 | |
| immune responses to vaginal or systemic infection of balb/c mice with herpes simplex virus type 2. | the temporal relationships among the humoral and cellular immune responses were defined in balb/c mice after vaginal or systemic infection with herpes simplex virus type 2 (hsv-2). after vaginal infection, mice showed evidence of clinical vaginitis on days 4 to 6 and hsv-2 replication was detected locally in the vaginal secretions, cervix, vagina, and uterus before the virus subsequently spread to the central nervous system. death from encephalitis occurred between 7 and 10 days after infection. ... | 1977 | 72115 |
| detection of herpes simplex virus-related antigens in the nuclei and cytoplasm of biochemically transformed cells with peroxidase/anti-peroxidase immunological staining and indirect immunofluorescence. | herpes simplex virus (hsv)-related antigens have been demonstrated in the nuclei and cytoplasm of human and mouse cells biochemically transformed by ultraviolet light-irradiated hsv. this was accomplished by using peroxidase/anti-peroxidase immunological staining and indirect immunofluorescence with rabbit antisera that had high neutralizing titers against the hsv-specific thymidine kinase activity and virus infectivity. hsv-1 antisera reacted with antigens in cells biochemically transformed by ... | 1977 | 71279 |
| occurrence of genital herpes simplex and cytomegalovirus infections in pregnancy. | a total of 244 pregnant women, 172 in the first and 72 in the third trimester, were screened for genital herpes simplex (hsv) and cytomegalovirus (cmv) infections using virus isolation, cytological examination and serological studies. in addition, immuno-fluorescence staining of exfoliated cervical cells was used for the detection of hsv infections. herpes simplex virus (hsv) could not be isolated and no characteristic hsv altered cells were found in cytological (papa) smears. cytomegalovirus wa ... | 1977 | 67744 |
| an immunofluorescence technique with counterstain on fixed cells for the detection of antibodies to human herpesviruses; antibody patterns in patients with hodgkin's disease and nasopharyngeal carcinoma. | an indirect immunofluorescence (if) test on fixed cells with evans' blue counterstain is described for all four human herpesviruses, i.e., herpes simplex virus type 1 and type 2 (hsv-1 and hsv-2), varicella-zoster virus (vzv), cytomegalovirus (cmv) and epstein-barr virus (ebv). comparison with immunodiffusion (id) for hsv-2 and with id and complement fixation (cf) for vzv and cmv demonstrated the specificity and high sensitivity of the if test. also introduced is a modification of the anti-compl ... | 1976 | 67099 |
| glycoproteins specified by herpes simplex virus type 1: their synthesis, processing and antigenic relatedness to hsv -2 glycoproteins. | 1975 | 65316 | |
| expression of an early, nonstructural antigen of herpes simplex virus in cell transformed in vitro by herpes simplex virus. | hyperimmune rabbit antiserum to an early, nonstructural herpes simplex virus type 2 (hsv-2)-induced polypeptide (vp143) reacted in immunofluorescence tests with a variety of cell lines transformed by hsv-2. cytoplasmic fluorescence was observed in 10 to 50% of hsv-2-transformed cells, whereas no fluorescence was observed in cells transformed by other oncogenic dna viruses or by a chemical carcinogen. vp143-specific reactivity could be absorbed from anti-vp143 serum with hsv-2-transformed cells b ... | 1977 | 64626 |
| serum antibodies to herpesvirus early antigens in patients with cervical carcinoma determined by anticomplement immunofluorescence technique. | sera from patients with carcinoma of the uterine cervix were used with the anticomplement immunofluorescence (acif) technique to develop a simple procedure for detection of antibodies to herpes simplex virus type 2 (hsv-2) early antigens. test cells used in the acif assay were hep-2 cells infected with hsv-2 sequentially treated with inhibitors of protein and of dna synthesis. the cells were first treated for 7 h with cycloheximide (100 mug/ml) and then for 3 h with hydroxyurea (150 mug/ml). in ... | 1976 | 62718 |
| herpes simplex virus type 2 and cancer of the prostate. | a seroepidemiologic study was performed to determine if there was an association between antibodies to herpes simplex virus type 2 and cancer of the prostate, similar to that reported for herpes simplex virus type 2 and cervical carcinoma. no significant difference in herpes simplex virus type 2 antibodies was seen between patients with cancer of the prostate and those with benign prostatic hypertrophy. further studies are needed to define the possible role of herpes simplex virus type 2 in the ... | 1976 | 62063 |
| in vivo and in vitro "markers" of human cervical intraepithelial neoplasia. | the human uterine cervix offers a unique opportunity to study the early lesions of squamous cell carcinoma, i.e., carcinoma in situ and dysplasia [combined as cervical intraepithelial neoplasia (cin)]. in vivo, the patients with cin have the epidemiological common denominators or "markers" of early onset of coitus, multiple sexual partners, 1st delivery before age 20, and antibodies to herpes simplex virus type 2 more frequently than do controls. the lesions themselves have specific epithelial a ... | 1976 | 58720 |
| serological and epidemiological considerations of the role of herpes simplex virus type 2 in cervical cancer. | to assess the possible biological significance of the observations that women with cervical cancer tend to be younger at first intercourse than control women, data from 1823 women were analyzed for the relationship between age at 1st intercourse and number of sex partners. women who were younger at first intercourse had more sex partners than did women who were older at first intercourse. the interdependence of age at first intercourse and number of sex partners does not exlude the possibility t ... | 1976 | 56227 |
| differential feulgen-deoxyribonucleic acid hydrolysis patterns of herpes simplex virus type 1 and type 2 infected cells. | infection of human embryonic lung cells with herpes simplex virus type 1 (hsv-1) and herpes simplex type 1 (hsv-2) resulted in: (a) qualitative (nuclear cytopathologic) alterations and quantitative (nuclear area) differences in infected compared to control nuclei; (b) increased feulgen-deoxyribonucleic acid (f-dna) amounts in infected cells, probably due to viral dna; (c) higher f-dna levels in hsv-2 infected cells; and (d) increased rates of f-dna hydrolysis in viral-infected as compared to uni ... | 1975 | 47871 |
| biological effects of 5-carboxy-2'-deoxyuridine: hydrolysis product of 5-trifluoromethyl-2'-deoxyuridine. | 5-carboxy-2'-deoxyuridine (5-cooh-2'-durd) is a product of the base-catalyzed hydrolysis of 5-trifluoromethyl-2'-deoxyuridine. hydrolysis of 5-trifluoromethyl-2'-deoxyuridine to 5-cooh-2'-durd in phosphate-buffered saline was kinetically first order and was ph dependent. at 37 degrees c and ph 7.0, 7.5, and 8.0, hydrolysis occurred with rate constants of 4.19 x 10(-5), 9.30 x 10(-5), and 1.61 x 10(-4) s(-1), respectively, with corresponding half-lives of 45.7, 20.6, and 11.9 h. 5-cooh-2'-durd in ... | 1978 | 28691 |
| increased susceptibility to infection with herpes simplex virus types 1 and 2 of cold-adapted l cells. | l cells (l-as subline) have been adapted to a temperature of 4 degrees c. in the cold-adapted cells, designated lc3, greater amounts of infectious herpes simplex virus types 1 (hsv-1) and 2 (hsv-2) were synthesized than in the original l-as cells or in another control l-cell line. two strains of hsv-1 reached higher infectious titres in lc3 cells grown at 36 degrees c than in those grown at 32 degrees c. the hsv-2 strain tested replicated in lc-3 cells grown at 32 degrees c better than at higher ... | 1978 | 26196 |
| measurement of antibodies to herpesvirus types 1 and 2 in human sera by microradioimmunoassay. | the binding of 125i-labelled anti-human antibodies against the fc igg fragment to unlabelled antiviral immunoglobulins in the surface of infected cells was used to quantitate antibodies against herpes simplex virus type (hsv-1) and type 2 (hsv-2) in sera from patients with cervix carcinoma. the microradioimmunoassay technique (micro-ria) proved to be 5-10 times more sensitive than the microneutralization test. antibody titres determined by micro-ria correlated with neutralizing antibody titres t ... | 1977 | 22235 |
| ribonucleotide reductase from herpes simplex virus (types 1 and 2) infected and uninfected kb cells: properties of the partially purified enzymes. | mammalian ribonucleotide reductase is a complex enzyme modified in its activity by a complex regulatory system involving adenosine triphosphate (atp) and deoxyribonucleoside triphosphates. infection of kb cells with herpes simplex virus (hsv) type 1 or 2 induces the formation of an altered ribonucleotide reductase. the properties of partially purified reductase from uninfected kb cells have been compared with the enzymes obtained from hsv-1 and hsv-2 infected kb cells. we found that the virus-in ... | 1977 | 18550 |