Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| physiological and ultrastructural effects of cadmium on wheat (triticum aestivum l.) leaves. | the effects of a 7-day exposure of 3-day-old wheat plantsto increasing cd concentrations are described, with special attention beinggiven to chloroplast ultrastructural changes, chlorophyll fluorescenceresponses, chlorophyll and nutrient concentration changes as well as growthchanges of the whole plant. the plants treated with 1 mm cd showed symptomsof heavy metal toxicity. the root, shoot-leaf length and the root, shoot-leafbiomass progressively decreased with increasing cd in nutrient solution ... | 1997 | 9069190 |
| in vitro assessment of acetic-acid-soluble proteins (glutenin) toxicity in celiac disease. | acetic-acid-soluble storage proteins from gluten of the bread wheat cv. sprint 3 were fractionated by adsorption chromatography on 2000 a controlled-pore glass (cpg) beads, and glutenin polymers with molecular mass higher than 10(7) da and free from monomeric gliadins were recovered. the glutenin polymers were found to consist of high-molecular-weight (hmw) and low-molecular-weight (lmw) glutenin subunits. peptic-tryptic (pt) digests of glutenins were examined for their agglutination activity on ... | 1996 | 9062852 |
| genetic and biochemical characterization of novel low molecular weight glutenin subunits in wheat (triticum aestivum l.). | two novel low molecular weight subunits of glutenin with relative molecular mass (m(r) values) of 30 and 32 kda were isolated from the seeds of hexaploid wheat and characterized at genetic and biochemical levels. among 115 indian bread wheat cultivars analysed, 40 had a narrow doublet of the new protein bands, 69 had a wide doublet, 3 had only the faster moving band of the doublet, and the remaining 3 cultivars had only the slower moving band. these subunits could be seen in the alkylated gluten ... | 1997 | 9061913 |
| regulated expression of a wheat germin gene in tobacco: oxalate oxidase activity and apoplastic localization of the heterologous protein. | wheat (triticum aestivum) germin is a homopentameric glycoprotein whose synthesis is allied with seed germination. germin pentamers show an unusual resistance to dissociation and possess an oxalate oxidase (oxo) activity. in order to increase our knowledge of germin gene expression, the function(s) of germin during development and possible uses in plant genetic engineering, an in vivo expression system is required. to this end, a gene for germin, named gf-2.8, was studied by expressing either pr ... | 1997 | 9049263 |
| plasmon analysis of triticum (wheat) and aegilops. 1. production of alloplasmic common wheats and their fertilities. | plasmons (= cytoplasms) of eight triticum species (ten accessions) and 24 aegilops species (36 accessions) have been introduced by repeated backcrosses to 12 genotypes of hexaploid, common wheat. at transfer problems such as crossing barrier, preferential transmission of a gametocidal or parthenogenesis-inducing chromosome, and mistagging of the material occurred, all of which hindered the plasmon transfer program. of the 552 genotype-plasmon combinations produced, 532 (96.4%) had reached the b1 ... | 1996 | 9037776 |
| polymerase chain reaction-single strand conformational polymorphism analysis of intra- and interspecific variations in organellar dna regions of aegilops mutica and related species. | in order to study the phylogeny of aegilops mutica in the genera of triticum and aegilops, variations in chloroplast and mitochondrial dna regions were investigated by polymerase chain reaction-single strand conformational polymorphism (pcr-sscp) analysis. nine lines, each of ae. mutica and ae. speltoides, were studied together with nine other triticum and aegilops species, including t. aestivum. by analyzing 9.7-kb chloroplast and 13.1-kb mitochondrial dna regions, a total of 268 bands were det ... | 1996 | 9037775 |
| characterization of dynamics of the psbd light-induced transcription in mature wheat chloroplasts. | dynamical aspects of three chloroplast promoters responding to change in light condition were examined in mature chloroplasts of wheat (triticum aestivum) by in vitro transcription. the wheat psbd/c operon has four distinct promoters, two of which named as d/c-3 and d/c-4 promoters dominantly function in mature chloroplasts to produce the mrnas encoding d2/cp43 and cp43 alone, respectively. activity of the d/c-3 promoter in mature chloroplasts was reduced to less than 30% by 24 h dark adaptation ... | 1997 | 9037145 |
| comparison of the nucleotide sequence of the coat protein open reading frame of nine isolates of wheat streak mosaic rymovirus. | wheat streak mosaic rymovirus (wsmv) is an important pathogen of wheat (triticum aestivum l.). the coat protein region of nine isolates of wsmv were cloned by rt-pcr using primers that were inclusive of nucleotides 398-1825 (1) and sequence analysis indicated four regions of variability among the isolates. | 1996 | 9035362 |
| localization of benzoxazinones that occur constitutively in wheat seedlings. | occurrence and localization of novel antimicrobial and antifeeding compounds in wheat, 2,4-dihydroxy-1,4-benzoxazin-3-one (diboa) and 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one (dimboa), and their glucosides, were examined by staining wheat plants (triticum aestivum l.) in the juvenile stage of growth by ferric chloride. the methanol extracts of the stained plant tissues gave a characteristic blue color, which was shown by spectroscopic and chromatographic analyses to be exclusively due to ben ... | 2017 | 9031524 |
| characterization of the translocated chromosome using fluorescence in situ hybridization and random amplified polymorphic dna on two triticum aestivum-thinopyrum intermedium translocation lines resistant to wheat streak mosaic or barley yellow dwarf virus. | fluorescence in situ hybridization (fish) was used to determine the breakpoint of the translocation chromosome in two bread wheat (triticum aestivum) germplasm lines with thinopyrum intermedium chromatin carrying resistance to either wheat streak mosaic virus (wsmv) or barley yellow dwarf virus (bydv). in addition, genome-specific random amplified polymorphic dna (rapd) markers were used to ascertain the genomic sources of the th. intermedium chromosome carrying the wsmv or bydv resistance. ci17 ... | 1996 | 9024975 |
| differential expression and properties of starch branching enzyme isoforms in developing wheat endosperm. | three forms of starch branching enzyme (be) from developing hexaploid wheat (triticum aestivum) endosperm have been partially purified and characterized. immunological cross-reactivities indicate that two forms (wbe-iad, 88 kd, and wbe-ib, 87 kd) are related to the maize be i class and that wbe-ii (88 kd) is related to maize be ii. comparison of the n-terminal sequences from wbe-iad and wbe-ii with maize and rice bes confirms these relationships. evidence is presented from the analysis of nullis ... | 1997 | 9008395 |
| [electrophoretic analysis of buckwheat flour compared with regular wheat flour]. | the composition of the alcohol soluble proteins (prolamins) obtained from buckwheat meal and common wheat flour by two procedures were analysed by electrophoresis at ph 3.1 and, after dissociation, in the presence of sodium dodecyl sulphate at ph 8. the profiles obtained from the prolamin fraction of buckwheat were very different, qualitatively and quantitatively, from those of the prolamin of common wheat. it is probable therefore that the adverse effects associated with the presence of wheat g ... | 1994 | 8984969 |
| hygromycin-resistant calli generated by activation and excision of maize ac/ds transposable elements in diploid and hexaploid wheat cultured cell lines. | to investigate the activation and transposition of maize transposable elements in wheat cultured cells, plasmid dnas containing the maize ac/ds elements located between the camv 35s promoter and a hygromycin b resistance gene (hph) were introduced into two wheat (triticum aestivum and triticum monococcum) cultured cell lines by microprojectile bombardment. in the first experiment, hph was activated by excision of the ac element, which encodes transposase, in the two wheat cell lines. in the seco ... | 1996 | 8983186 |
| cloning and characterization of a highly repeated dna sequence in hordeum vulgare l. | a novel repetitive dna sequence, r10hvcop, has been identified in the barley (hordeum vulgare l.) genome. this 830 base pair (bp) dna sequence has a 606-bp open reading frame and is present as approximately 1.96 x 10(5) copies per haploid barley genome. southern blot analysis revealed that repetitive dna elements containing r10hvcop and related sequences were dispersed within the barley chromosomes. sequences similar to r10hvcop were also found in wheat (triticum aestivum l.), rye (secale cereal ... | 1996 | 8983185 |
| changes in abundance of an abscisic acid-responsive, early cysteine-labeled metallothionein transcript during pollen embryogenesis in bread wheat (triticum aestivum). | a clone for an embryoid-abundant, early cysteine-labeled metallothionein (ecmt) gene has been isolated from a wheat pollen embryoid cdna library. the transcript of this gene was only expressed in embryogenic microspores, pollen embryoids, and developing zygotic embryos of wheat. accumulation of the ecmt mrna showed a direct and positive correlation with an increase of the plant hormone, abscisic acid (aba) in developing pollen embryoids. treating cultures with an inhibitor of aba biosynthesis, f ... | 1996 | 8980534 |
| chromium uptake and toxicity effects on growth and metabolic activities in wheat, triticum aestivum l. cv. up 2003. | chromium (cr) at graded levels when added in sand culture of wheat (t. aestivum l. cv. up2003) under glasshouse conditions resulted in reduction in biomass, chlorophyll and activities of catalase and peroxidase while enhanced acid phosphatase and ribonuclease activities. elevated levels of cr supply significantly reduced the concentration of inorganic phosphorus. with an increase in cr supply the uptake of chromium also increased significantly in different plant parts especially in roots. above ... | 1996 | 8979507 |
| identification and high-density mapping of gene-rich regions in chromosome group 1 of wheat. | we studied the distribution of genes and recombination in wheat (triticum aestivum) group 1 chromosomes by comparing high-density physical and genetic maps. physical maps of chromosomes 1a, 1b, and 1d were generated by mapping 50 dna markers on 56 single-break deletion lines. a consensus physical map was compared with the 1d genetic map of triticum tauschii (68 markers) and a triticeae group 1 consensus map (288 markers) to generate a cytogenetic ladder map (clm). most group 1 markers (86%) were ... | 1996 | 8978071 |
| distribution of lectin binding sites in human bone marrow. identification by use of an ultrastructural postembedding technique. | the purpose of this ultrastructural study was to detect various carbohydrate residues on mature elements of the major human haematopoietic cell lines (granulopoiesis, erythropoiesis and megakaryopoiesis), sinus endothelium and plasma cells under comparable experimental conditions. marrow specimens were processed according to a modified postembedding technique with unicryl as embedding resin. a broad panel of 10 digoxigenin (dig)-conjugated lectins was applied for staining and specificity was eva ... | 1996 | 8933737 |
| characterization of the calmodulin gene family in wheat: structure, chromosomal location, and evolutionary aspects. | calmodulin is a ubiquitous transducer of calcium signals in eukaryotes. in diploid plant species, several isoforms of calmodulin have been described. here, we report on the isolation and characterization of calmodulin cdnas corresponding to 10 genes from hexaploid (bread) wheat (triticum aestivum). these genes encode three distinct calmodulin isoforms; one isoform is novel in that it lacks a conserved calcium binding site. based on their nucleotide sequences, the 10 cdnas were classified into fo ... | 1996 | 8917311 |
| conversion of d-hamamelose into 2-carboxy-d-arabinitol and 2-carboxy-d-arabinitol 1-phosphate in leaves of phaseolus vulgaris l. | [1-14c]hamamelose (2-hydroxymethyl-d-ribose) was synthesized by reaction of ribulose 5-phosphate with potassium [14c]cyanide, catalytic hydrogenation of the resulting cyanohydrin, and dephosphorylation of the product. its identity was established by a chromatographic comparison with hamamelose isolated from the bark of witch hazel (hamamelis virginiana l.). following vacuum infiltration of the [1-14c]hamamelose into leaf discs from phaseolus vulgaris l., 14c-labeled 2carboxy-d-arabinitol (ca) an ... | 1996 | 8900161 |
| a low-molecular-mass gtp-binding protein in the cytosol of germinated wheat embryos. | a low-molecular-mass protein able to bind gtp in both native and sds-denaturating conditions was detected in the cytosol of embryos from wheat (triticum aestivum l.) seeds germinated for 40 h. the protein fulfilled most of the distinguishing criteria common to eukaryotic small gtp-binding proteins. it retained the ability to bind gtp after sds/page and nitrocellulose blotting. the protein eluted from sephadex g-200 gel filtration with a ve/vo value corresponding to a molecular mass of 18 kda, wh ... | 1996 | 8898918 |
| severity of mutant phenotype in a series of chlorophyll-deficient wheat mutants depends on light intensity and the severity of the block in chlorophyll synthesis. | analyses of a series of allelic chlorina mutants of wheat (triticum aestivum l.), which have partial blocks in chlorophyll (chl) synthesis and, therefore, a limited chl supply, reinforce the principle that chl is required for the stable accumulation of chl-binding proteins and that only reaction centers accumulate when the supply of chl is severely limited. depending on the rate of chl accumulation (determined by the severity of the mutation) and on the rate of turnover of chl and its precursors ... | 1996 | 8883392 |
| constitutively hyposialylated human t-lymphocyte clones in the tn-syndrome: binding characteristics of plant and animal lectins. | previously, beta 1,3-galactosyltransferase-deficient (tn+) and normal (tf+) t-lymphocyte clones have been established from a patient suffering from tn-syndrome [thurnher et al. (1992) eur j immunol 22: 1835-42]. tn+ t lymphocytes express only tn antigen galnac alpha 1-o-r) while other o-glycan structures such as sialosyl-tn (neu5ac alpha 2,6galnac alpha 1-o-r) or tf (gal beta 1-3galnac alpha 1-o-r) antigens are absent from these cells as shown by flow cytometry using specific mabs for tf and sia ... | 1996 | 8872113 |
| a cytogenetic analysis of rye introgression into a portuguese bread wheat cultivar barbela. | 1996 | 8871830 | |
| relations between aggregative, viscoelastic and molecular properties in gluten from genetic variants of bread wheat. | glutens differing in their low- and high-molecular-weight glutenin subunits (lmw-gs and hmw-gs) were extracted from genetic variants of bread wheat. their composition was analysed by electrophoresis, the glutenin size distribution was determined by size-exclusion chromatography. rheological measurements in the dynamic regime and electron spin resonance spin-labelling experiments gave data on the viscoelasticity and the protein flexibility of the glutens, respectively. in glutens differing in the ... | 1996 | 8852755 |
| frequencies and sequence characteristics of di-, tri-, and tetra-nucleotide microsatellites in wheat. | microsatellites have emerged as an important source of genetic markers for eukaryotic genomes. in this report, two wheat (triticum aestivum l.) genomic libraries were screened for several di-, tri-, and tetranucleotide tandem repeats. clones containing (ac)n, (ag)n, (tct)n, and (ttg)n repeats were isolated and sequenced. on average, there was one (ac)n microsatellite every 292 kbp and one (ag)n microsatellite every 212 kbp. the trinucleotide tandem repeats (tct)n and (ttg)n were about 10 times l ... | 1996 | 8851802 |
| structural and antifungal properties of a pathogenesis-related protein from wheat kernel. | we have purified and characterized a protein from the water-soluble fraction of wheat kernel (triticum aestivum cv. s. pastore) consisting of a single polypeptide chain blocked at its n-terminus by a pyroglutamate residue; the complete amino acid sequence has been determined by automated sequence analysis performed on peptide fragments obtained by enzymatic hydrolyses of the protein. homology studies have shown that this protein is very similar (97% sequence identity) to the previously character ... | 1996 | 8838588 |
| growth and photosynthetic responses of wheat plants grown in space. | growth and photosynthesis of wheat (triticum aestivum l. cv super dwarf) plants grown onboard the space shuttle discovery for 10 d were examined. compared to ground control plants, the shoot fresh weight of space-grown seedlings decreased by 25%. postflight measurements of the o2 evolution/photosynthetic photon flux density response curves of leaf samples revealed that the co2-saturated photosynthetic rate at saturating light intensities in space-grown plants declined 25% relative to the rate in ... | 1996 | 8819868 |
| aging of the human endometrium: peri-implantation phase endometrium does not show any age-dependent variation in lectin binding. | to evaluate if the peri-implantation endometrium shows age variations in lectin patterns, which suggest possible age variations in embryo-maternal recognition. | 1996 | 8801134 |
| difference in glycohistochemical lectin staining of collagen fibers in the corpora cavernosa of normal and impotent men. | the objectives of this study were to investigate the value of glycohistochemical staining with three lectin types specific to a particular glycan structure (arachis hypogaea [pna], triticum vulgare [wga], and concanavalin a [con a]) as a method of defining possible changes in the collagen structure in the corpora cavernosa in potent and impotent men. the study group consisted of 4 normal potent men and 22 men with various etiologies of impotence. the quantitative histochemical measurements were ... | 2007 | 8792209 |
| covalent dimerization of ribulose bisphosphate carboxylase subunits by uv radiation. | the effect of uv radiation (uv-a, uv-b and uv-c) on ribulose bisphosphate carboxylase from a variety of plant species was examined. the exposition of plant leaves or the pure enzyme to uv radiation produced a uv-dependent accumulation of a +5 kda polypeptide (p65). different approaches were utilized to elucidate the origin and structure of p65: electrophoretic and fluorographic analyses of 35s-labelled ribulose bisphosphate carboxylase exposed to uv radiation and immunological experiments using ... | 1996 | 8761476 |
| from image processing to classification: iv. classification of electrophoretic patterns by neural networks and statistical methods enable quality assessment of wheat varieties for breadmaking. | the end-use quality of products made from doughs consisting of wheat flour and water is often dependent upon the storage (gluten) proteins of the grain endosperm. today the electrophoretic patterns of the high molecular weight (hmw) glutenin subunits are used for quality selections in wheat breeding programs in several countries. in this study, we used two multivariate techniques to classify digitized patterns from isoelectric focusing of gliadins and glutenins: a two-layered neural network arch ... | 1996 | 8738329 |
| secondary structure of nadph: protochlorophyllide oxidoreductase examined by circular dichroism and prediction methods. | to study the secondary structure of the enzyme nadph: protochlorophyllide oxidoreductase (pcor), a novel method of enzyme isolation was developed. the detergent isotridecyl poly-(ethylene glycol) ether (genapol x-080) selectively solubilizes the enzyme from a prolamellar-body fraction isolated from wheat (triticum aestivum l.). the solubilized fraction was further purified by ion-exchange chromatography. the isolated enzyme was studied by fluorescence spectroscopy at 77 k, and by cd spectroscopy ... | 1996 | 8713084 |
| primary structure of omega-hordothionin, a member of a novel family of thionins from barley endosperm, and its inhibition of protein synthesis in eukaryotic and prokaryotic cell-free systems. | a new sulfur-rich basic polypeptide, so called omega-hordothionin, has been isolated from barley endosperm by extractions with nacl and ammonium bicarbonate followed by reverse-phase high performance liquid chromatography. purified omega-hordothionin was found to be homogeneous by sds/polyacrylamide gel electrophoresis, n-terminal amino-acid sequencing and electrospray-ionization mass spectrometric analysis. the complete primary structure of omega-hordothionin was determined by automatic degrada ... | 1996 | 8706720 |
| cdna encoding a wheat (triticum aestivum cv. chinese spring) glycine-rich rna-binding protein. | a wheat cdna encoding a glycine-rich rna-binding protein, whgrp-1, was isolated. whgrp-1 contains two conserved domains, the rna-binding motif (rnp motif) combined with a series of glycine-rich imperfect repeats, characteristic of a conserved family of plant rna-binding proteins. northern analysis revealed that whgrp-1 mrna accumulates to high levels in roots and to lower levels in leaves of wheat seedlings, whgrp-1 mrna accumulation is not enhanced by exogenous abscisic acid in seedlings and ac ... | 1996 | 8704137 |
| [rapd markers for wheat chromosomes in langdon disomic substitution lines]. | optimal conditions were developed for a random amplified polymorphio dna (rapd) assay of hexaploid bread wheat and tetraploid durum wheat. amplitaq stoffcl fragment was found to be better than taq dna polymerase in generating rapds. studies on chromosome specific rapd markers of the a- and b- and d-genome were performed using the complete set of langdon disomic substitution lines and the parental lines (langdon and chinese spring) as templete. seven out of twelve arbitrary primers (all operon 10 ... | 1996 | 8695176 |
| a histological, lectin and s-100 histochemical study of the developing prenatal human sublingual salivary gland. | lectin and s-100 protein histochemistry during fetal growth and development (10-38th gestational weeks) of these glands was studied. the histological development of glandular structures followed the known pattern for other salivary glands. using biotinylated lectins ulex europeus-i, dolichos biflorus, glycine maximus (soyabean), helix pomatia, arachis hypogaea (peanut) and triticum vulgare (wheatgerm), the binding level and, by implication, the concentration of associated specific oligosaccharid ... | 1995 | 8670026 |
| molecular mapping of wheat: major genes and rearrangements in homoeologous groups 4, 5, and 7. | a molecular-marker linkage map of hexaploid wheat (triticum aestivum l. em. thell) provides a framework for integration with teh classical genetic map and a record of the chromosomal rearrangements involved in the evolution of this crop species. we have constructed restriction fragment length polymorphism (rflp) maps of the a-, b-, and d-genome chromosomes of homoeologous groups 4, 5, and 7 of wheat using 114 f7 lines from a synthetic x cultivated wheat cross and clones from 10 dna libraries. ch ... | 1995 | 8647405 |
| the trnatyr multigene family of triticum aestivum: genome organization, sequence analyses and maturation of intron-containing pre-trnas in wheat germ extract. | southern analysis of triticum dna has revealed that nuclear trnatyr genes are dispersed at a minimum of 16 loci in the genome. we have isolated six independent trnatyr genes from a triticum aestivum library in addition to three known members of the triticum trnatyr family. four of the sequenced trnatyr genes code for triticum trna tyr and two code for trna2tyr. three genes encode trnastyr which carry one or two nucleotide substitutions as compared to the conventional genes. the nine triticum trn ... | 1996 | 8617358 |
| evaluation of sensitivity of flow cytometry in detecting aneuploidy in wheat using disomic and ditelosomic wheat-rye addition lines. | flow cytometric dna analysis was used to study changes in nuclear dna content induced by the addition of complete or telosomic rye chromosomes into the genome of common wheat (triticum aestivum l.). the dna content of each addition line was determined by comparison with an internal reference value and was expressed as a difference with respect to the original wheat parental line. a 1.84% difference in the dna content could be detected. nuclei were flow sorted and the presence of rye chromatin in ... | 1995 | 8608738 |
| a recombinant wheat serpin with inhibitory activity. | a full-length clone encoding the wheat (triticum aestivum l.) serpin wsz1 was isolated from a cdna library based on mrna from immature grain. the 398 amino acid sequence deduced from the cdna was corroborated by sequencing cnbr peptides of wsz1 purified from resting grain. wsz1 belongs to the subfamily of protein z-type serpins and the amino acid sequence is 70% identical with the barley serpins bsz4 and bszx and 27-33% identical with human serpins such as alpha 1-proteinase inhibitor, antithrom ... | 1996 | 8605317 |
| the inhibition of human salivary alpha-amylase by type ii alpha-amylase inhibitor from triticum aestivum is competitive, slow and tight-binding. | a kinetic analysis of the inhibition of human salivary alpha-amylase (ec 3.2.1.1) by wheat seed (triticum aestivum) type ii alpha-amylase inhibitor revealed the inhibition was slow and tight-binding. the inhibition was competitive with an inhibition binding constant of the alpha-amylase inhibitor for alpha-amylase of 0.29 nm. the km of alpha-amylase for soluble starch (calculated per mole of alpha-1,4 linked maltose residues) was 5.87 mm. | 1995 | 8583253 |
| wheat ribosome-inactivating proteins: seed and leaf forms with different specificities and cofactor requirements. | distinct forms of ribosome-inactivating proteins were purified from wheat (triticum aestivum l.) germ and leaves and termed tritin-s and tritin-l, respectively. these differ in size and charge and are antigenically unrelated. they are both rna n-glycosidases which act on 26s rrna in native yeast (saccharomyces cerevisiae) ribosomes by the removal of a3024 located in a universally conserved sequence in domain vii which has previously been identified as the site of action of ricin a-chain. tritin- ... | 1995 | 8555963 |
| ultrastructural localization of carbohydrates in reichert's membrane of the mouse. | in the present investigation, we examined the role of trophoblast and parietal endoderm cells in the synthesis of carbohydrate-containing components of reichert's membrane. to eliminate the function of reichert's membrane as a filter between maternal and embryonal tissues we carried out our examination under in vitro conditions. parietal yolk sac from mouse embryos on day 9 post coitum (p.c.) were cultivated for 0 to 5 days. because tannic acid enables a complex formation between carbohydrates a ... | 1993 | 8513488 |
| nucleotide sequence of a genomic gene encoding tritin, a ribosome-inactivating protein from triticum aestivum. | a genomic gene of tritin, a ribosome-inactivating protein (rip) from triticum aestivum, was cloned using a barley rip gene as a probe. the 5'-non-coding region has potential tata boxes and three sequences homologous to the binding sequence of the transcriptional activator protein opaque-2 which activates maize rip gene expression. the cloned dna encoded tritin consists of 275 amino acids with no secretion signal sequence. the coding region of tritin was expressed in escherichia coli using lac pr ... | 1993 | 8499616 |
| 8-vinyl reduction and chlorophyll a biosynthesis in higher plants. | a technique involving solid-phase extractions and polyethylene h.p.l.c. suitable for the routine compositional analysis of the total protochlorophyllide pool of plants is described. the resynthesis kinetics of the individual components of the pool have been studied in briefly illuminated etiolated tissue of wheat (triticum aestivum) and cucumber (cucumis sativus) during subsequent redarkening. the data are interpreted in terms of a precursor-product relationship between the di- and mono-vinyl an ... | 1993 | 8489519 |
| identification of three wx proteins in wheat (triticum aestivum l.). | nullisomic analysis of waxy (wx) protein of hexaploid wheat (triticum aestivum l.) cv. "chinese spring" using two-dimensional polyacrylamide gel electrophoresis revealed that three wx loci, wx-a1, wx-b1, and wx-d1, located on chromosome arms 7as, 4al, and 7ds, produce three distinct wx subunit groups, subunit group-a (sga), sgb, and sgd, respectively. sga has a higher molecular weight and a more basic isoelectric point (pi) than the other two. sgb and sgd have the same molecular weight but a sli ... | 1993 | 8471025 |
| comparative analysis of a recombining-repeat-sequence family in the mitochondrial genomes of wheat (triticum aestivum l.) and rye (secale cereale l.). | the mitochondrial genomes of wheat and rye each contain a three-member family of recombining repeat sequences (the "18s/5s repeat") that encode genes for 18s and 5s rrnas (rrn18 and rrn5) and trna(fmet) (trnfm). here we present, for wheat and rye, the sequence and boundaries of the "common sequence unit" (csu) that is shared between all three repeat copies in each species. the wheat csu is 4,429 base-pairs long and contains (in addition to trnfm, rrn18 and rrn5) a putative promoter, three trna-l ... | 1993 | 8435855 |
| cytoplasmic male sterility and fertility restoration in wheat are not associated with rearrangements of mitochondrial dna in the gene regions for cob, coxii, or coxi. | in comparing the genetic organization and exploring the molecular basis of cytoplasmic male sterility (cms) in wheat, mitochondrial dnas (mtdna) from triticum aestivum, t. timopheevi, cms alloplasmic wheat with t. aestivum nucleus and t. timopheevi mitochondria, and fertility-restored lines were compared by hybridization analysis with specific probes for three gene regions: coxii, cob, and coxi. minor differences between t. aestivum- and t. timopheevi-derived sources were found for gene regions ... | 1993 | 8425048 |
| antinutritive effects of wheat-germ agglutinin and other n-acetylglucosamine-specific lectins. | incorporation of n-acetylglucosamine-specific agglutinins from wheat germ (triticum aestivum; wga), thorn apple (datura stramonium) or nettle (urtica dioica) rhizomes in the diet at the level of 7 g/kg reduced the apparent digestibility and utilization of dietary proteins and the growth of rats, with wga being the most damaging. as a result of their binding and endocytosis by the epithelial cells of the small intestine, all three lectins were growth factors for the gut and interfered with its me ... | 1993 | 8399111 |
| purification and characterization of (1-->3, 1-->4)-beta-glucan endohydrolases from germinated wheat (triticum aestivum). | a (1-->3, 1-->4)-beta-glucan 4-glucanohydrolase [(1-->3, 1-->4)-beta-glucanase, ec 3.2.1.73] was purified to homogeneity from extracts of germinated wheat grain. the enzyme, which was identified as an endohydrolase on the basis of oligosaccharide products released from a (1-->3, 1-->4)-beta-glucan substrate, has an apparent pi of 8.2 and an apparent molecular mass of 30 kda. western blot analyses with specific monoclonal antibodies indicated that the enzyme is related to (1-->3, 1-->4)-beta-gluc ... | 1993 | 8358032 |
| chromosomal locations of the genes for histones and a histone gene-binding protein family hbp-1 in common wheat. | the chromosomal locations of the genes in common wheat that encode the five histones and five members of the hbp (histone gene-binding protein)-1 family were determined by hybridizing their cloned dnas to genomic dnas of nullitetrasomic and telosomic lines of common wheat, triticum aestivum cv. chinese spring. the h1 and h2a genes are located on different sets of homoeologous chromosomes or chromosome arms, namely, 5a, 5b and 5d, and 2as, 2bs and 2ds, respectively. genes for the other histones, ... | 1993 | 8343598 |
| storage changes in the quality of sound and sprouted flour. | sound and sprouted flours (24 and 48 hr) from bread wheat (wl-1562), durum wheat (pbw-34) and triticale (tl-1210) were stored at room temperature (34.8 degrees c) and relative humidity (66.7%) for 0, 45, 90 and 135 days to assess the changes in physico-chemical and baking properties. protein, gluten, sedimentation value, starch and crude fat decreased during storage in all the samples; however, the decrease was more in sprouted flours. free amino acids, proteolytic activity, diastatic activity a ... | 1993 | 8332585 |
| studies on the constancy of the blue and green fluorescence yield during the chlorophyll fluorescence induction kinetics (kautsky effect). | blue (f450) and green (f530) leaf fluorescence were studied together with the red chlorophyll fluorescence (emission maxima f690 and f735) during light-induced chlorophyll fluorescence induction kinetics (kautsky effect) in predarkened leaves of wheat (triticum aestivum l.) and soybean (glycine max l.). the intensity of the red chlorophyll fluorescence decreased from maximum fluorescence fm to steady-state fluorescence fs, and the fluorescence ratio f690/f735 decreased by about 10% from fm to fs ... | 1993 | 8310130 |
| a 'zebra' chromosome arising from multiple translocations involving non-homologous chromosomes. | an alloplasmic wheat line carrying a 'zebra' chromosome z5a was isolated from the derivatives of an elymus trachycaulus x triticum aestivum cv chinese spring hybrid. chromosome z5a was named zebra because of its striped genomic in situ hybridization pattern. z5a consists of four chromosome segments derived from e. trachycaulus and four chromosome segments, including the centromere, from wheat. the short arm of z5a paired with the telocentric chromosome 1h(t)s of e. trachycaulus and the long arm ... | 1993 | 8306822 |
| new cytochrome p450-dependent reactions from wheat: terminal and sub-terminal hydroxylation of oleic acid by microsomes from naphthalic acid anhydride and phenobarbital induced wheat seedlings. | incubation of the microsomal fraction from etiolated wheat shoots (triticum aestivum l. cv etoile de choisy) with [1-14c]oleic acid led to the formation of three polar metabolites which were identified as 18-, 17- and 16-hydroxyoleic acids by gas chromatography/mass spectra analysis. they were generated in a molar ratio of 1.4/4.6/4, respectively. terminal and sub-terminal hydroxylation of oleic acid and the cytochrome p450 content were strongly enhanced in microsomes from wheat shoots treated w ... | 1994 | 8297392 |
| differential induction of distinct glutathione-s-transferases of wheat by xenobiotics and by pathogen attack. | we have previously characterized a pathogen-induced gene from wheat (triticum aestivum l.) that was named gsta1 based on sequence similarities with glutathione-s-transferases (gsts) of maize (r. dudler, c. hertig, g. rebmann, j. bull, f. mauch [1991] mol plant microbe interact 4: 14-18). we have constructed a full-length gsta1 cdna by combinatorial polymerase chain reaction and demonstrate by functional expression of the cdna in escherichia coli that the gsta1-encoded protein has gst activity. a ... | 1993 | 8278547 |
| tobacco plants transformed with the o-acetylserine (thiol) lyase gene of wheat are resistant to toxic levels of hydrogen sulphide gas. | o-acetylserine (thiol) lyase (ec4.2.99.8) is the key enzyme in the cysteine biosynthetic pathway of plants and prokaryotes. the gene, cys1, encoding this enzyme was isolated from a wheat (triticum aestivum l.) cdna library, and its deduced amino acid sequence found to show 53% sequence identity with the o-acetyl-serine (thiol) lyase of escherichia coli and salmonella typhimurium. the deduced peptide consists of 325 amino acids (34.1 kda), contains a conserved motif for the binding of pyridoxal p ... | 1993 | 8275097 |
| cloning, characterization and import studies on protochlorophyllide reductase from wheat (triticum aestivum). | a full-length protochlorophyllide reductase clone (pwr5) has been isolated from a dark-grown wheat (triticum aestivum) cdna library generated in the phage vector lambda gt10. comparison of the sequence of pwr5 with published sequences indicates a high degree of conservation of the structure of the mature protein amongst species but with the structure of the transit peptide less highly conserved. within the cereals, the structure of the complete preprotein shows a remarkable degree of sequence ho ... | 0 | 8250847 |
| molecular cloning and cdna sequencing of endoxyloglucan transferase, a novel class of glycosyltransferase that mediates molecular grafting between matrix polysaccharides in plant cell walls. | endoxyloglucan transferase is a novel class of glycosyltransferase recently purified from vigna angularis (nishitani, k,, and tominaga, r. (1992) j. biol. chem. 267, 21058-21064). this enzyme is the first transferase identified that catalyzes molecular grafting between polysaccharide cross-links in the cell wall matrix and participates in reconstruction of the network structure in the cell wall. based on the nh2-terminal amino acid sequence information of the purified transferase, we have here c ... | 1993 | 8244968 |
| studies on the refrigerated storage of wheat (triticum aestivum). 2. ergosterol, xanthomegnin, viomellein and brevianamide a after inoculation with penicillium viridicatum. | wheat seed was adjusted to 18, 20, 22, 24 and 26% moisture content (m.c.), and stored for 240 days at 4 or 10 degrees c following inoculation with a strain of penicillium viridicatum producing the toxins, xanthomegnin (xa), viomellein (vio), and brevianamide a (ba). wheat kernels were not sterilized before inoculation. the concentration of ergosterol (erg), a chemical indicator of fungal biomass, remained constant at 18% m.c./4 degrees c, but increased under the other conditions. the time before ... | 1993 | 8237145 |
| isolation and analysis of a cdna clone encoding the small subunit of adp-glucose pyrophosphorylase from wheat. | a full-length cdna clone from hexaploid bread wheat, encoding the small subunit of adp-glucose pyrophosphorylase, has been isolated from an endosperm cdna library. the cdna insert has an open reading frame which encodes a protein of 473 amino acids (52.1 kda). the presence of a chloroplast/amyloplast transit peptide of 22 amino acids is proposed. the deduced amino acid sequence exhibits a high degree of homology with the small subunit adp-glucose pyrophosphorylase proteins from rice (with 90% of ... | 1993 | 8219053 |
| distribution and properties of major ribosome-inactivating proteins (28 s rrna n-glycosidases) of the plant saponaria officinalis l. (caryophyllaceae). | we have studied the distribution of the protein synthesis inhibitory activity in the tissues of saponaria officinalis l. (caryophyllaceae). seven major saporins, ribosome-inactivating proteins, were purified to apparent homogeneity from leaves, roots and seeds using a new procedure of rips isolation including ion-exchange and hydrophobic chromatography. they all catalysed the depurination of rat liver ribosomes, which generate the endo's diagnostic rrna fragment upon treatment with acid aniline, ... | 1993 | 8218413 |
| lectins as markers of rumen epithelial cell differentiation. | lectins of different carbohydrate specificities (gna (galanthus nivalis), con a (canavalia ensiformis), vfl (vicia faba), psl (pisum sativum), lca (lens culinaris), pna (arachis hypogaea; with or without prior neuraminidase treatment), wga (triticum vulgare), sba (glycine max), uea-i (ulex europaeus), lpa (limulus polyphemus), bs-i b4 (bandeiraea simplicifolia, isolectin b4)) were explored for use as differentiation markers of rumen epithelial cells in vivo and in vitro. lectins specific for man ... | 1994 | 8206789 |
| amplification of dna sequences in wheat and its relatives: the dgas44 and r350 families of repetitive sequences. | the sequence of a triticum tauschii genomic clone representing a family of d-genome amplified dna sequences, designated dgas44, is reported. the dgas44 sequence occurs on all chromosomes of the d genome of wheat, triticum aestivum, and in situ hybridization revealed it to be evenly dispersed on all seven chromosome pairs. an internal hindiii fragment of dgas44, designated dgas44-3, defines the highly amplified region that is specific to the d genome. the polymerase chain reaction was used to amp ... | 1994 | 8200519 |
| effects of nitrite and sulfate on k+ uptake and growth of wheat and cucumber seedlings. | the uptake of k+ ion was studied in the roots of wheat (triticum aestivum l. cv. gk szeged) and cucumber (cucumis sativus l. cv. budai csemege) seedlings grown in nutrient solution under nitrite and sulfate stress conditions. seedlings pretreated with 1 or 10 mm nano2 absorbed more k+(86rb) than those treated with 0.1 mm nano2. however, the posteffect was influenced considerably by na2so4 treatment. the results suggest that at least a partial feed-back regulation of k+ uptake may occur. however, ... | 1993 | 8184628 |
| digestibility, nitrogen utilization, and voluntary intake of ensiled crab waste-wheat straw mixtures fed to sheep. | crab waste and wheat (triticum aestivum l.) straw mixtures, ensiled with different additives, were evaluated in metabolism and palatability trials. crab waste and straw were mixed in proportions of 1:1, wet basis, with 20% water and different additives, and ensiled in 210-l metal drums double-lined with polyethylene bags. thirty crossbred wethers (40 kg initial bw) were fed a 1) basal diet consisting of 75% orchardgrass (dactylis glomerata l.) hay and 25% concentrate, 2) ensiled crab waste-wheat ... | 1994 | 8181970 |
| isolation of a new species-specific repetitive sequence from thinopyrum elongatum and its use in the studies of alien translocations. | a new repetitive sequence that is extremely abundant and well dispersed in the thinopyrum elongatum genome but present in low-copy number in wheat (triticum aestivum l.) has been isolated. this repeat and a th. elongatum repeat isolated in another laboratory were used to identify cosmid genomic clones containing the repeats and, thus, putatively located on a th. elongatum/t. aestivum translocation arm. most of the selected cosmids contained single- or low-copy sequences, making them potentially ... | 1994 | 8181740 |
| phylogenetic relationships of 10 grass species: an assessment of phylogenetic utility of the internal transcribed spacer region in nuclear ribosomal dna in monocots. | entire sequences of the internal transcribed spacers (itss) and 5.8s subunit of nuclear ribosomal dna (nrdna) were obtained from nine grass species by direct double-stranded sequencing of polymerase chain reaction (pcr) amplified dna fragments. these sequences from subfamily pooideae (triticum aestivum, crithodium monococcum, sitopsis speltoides, hordeum vulgare, secale montanum, avena longiglumis, bromus inermis, brachypodium distachyon) and subfamily panicoideae (sorghum bicolor) together with ... | 1994 | 8181731 |
| introgression of elymus trachycaulus chromatin into common wheat. | a number of wheat-elymus trachycaulus (2n = 4x = 28, genomically stsththt) chromosome addition, substitution, and translocation lines were isolated from the derivatives of an e. trachycaulus x wheat hybrid. eighteen out of a total of 28 chromosome arms of e. trachycaulus were recovered in the addition lines. the genomic affinity of individual e. trachycaulus chromosomes was analysed by comparative chromosome banding and in situ hybridization using genome-specific repetitive dna sequences as prob ... | 1994 | 8162318 |
| voltage-dependent ca2+ influx into right-side-out plasma membrane vesicles isolated from wheat roots: characterization of a putative ca2+ channel. | we report on the identification of a voltage-dependent ca2+ transport system that mediates ca2+ influx across the plasma membrane (pm) of wheat (triticum aestivum) root cells. the experimental approach involved the imposition of transmembrane electrical potentials (via k+ diffusion potentials) in populations of purified, right-side-out pm vesicles isolated from wheat roots. using 45ca2+ to quantify ca2+ influx into the pm vesicles, the voltage-dependent characteristics of ca2+ transport were fou ... | 1994 | 8159772 |
| in vitro starch and protein digestibility and iron availability in weaning foods as affected by processing methods. | in the present investigation, four weaning foods were formulated using locally available cereals and pulses such as wheat (triticum aestivum), barley (hordeum vulgare) and green gram (vigna radiata). cereal, pulse and jaggery were used in the proportion of 70:30:25. domestic processing technique like roasting and malting were used to process cereals and pulses for development of weaning foods. all the four blends had a nutrient composition within the range prescribed by the indian standard insti ... | 1994 | 8153067 |
| forage and splanchnic tissue mass in growing lambs: effects of dietary forage levels and source on splanchnic tissue mass in growing lambs. | forty-two crossbred lambs (33.4 kg initial body weight; twenty-four wethers and eighteen ewes) were used in a 42 d experiment with a 2 x 3 factorial treatment arrangement to determine effects of forage level and source on splanchnic tissue mass. diets were 250 and 750 g/kg of chopped lucerne (medicago sativa) (a), ryegrass (lolium multiflorum)-wheat (triticum aestivum) (rw) or bermudagrass (cynodon dactylon) (b) hay, with the remainder being maize-based concentrate. five lambs per treatment were ... | 1994 | 8142327 |
| a (1-->3,1-->4)-beta-glucan-specific monoclonal antibody and its use in the quantitation and immunocytochemical location of (1-->3,1-->4)-beta-glucans. | monoclonal antibodies were raised against a (1-->3,1-->4)-beta-glucan-bovine serum albumin (bsa) conjugate. one antibody (bg1) selected for further characterization, was specific for (1-->3,1-->4)-beta-glucan, displaying no binding activity against a (1-->3)-beta-glucan-bsa conjugate and minimal binding against a cellopentaose-bsa conjugate. a range of oligosaccharides was prepared by enzymatic digestion of (1-->3,1-->4)-beta-glucan, purified by size exclusion chromatography and characterized by ... | 1994 | 8130794 |
| identification, characterization, and analysis of cdna and genomic sequences encoding two different small heat shock proteins in hordeum vulgare. | in vitro translation of mrnas prepared from barley (hordeum vulgare) seedlings (cv. onice) exposed at 40 degrees c directed the synthesis of major heat shock proteins (hsps) with molecular masses of 80-90, 70, 42 and 16-22 kda. a cdna library prepared from the 40 degrees c mrnas and screened by differential hybridization led to the isolation of heat shock specific sequences. one of these (hv hsp18) was confirmed by hybrid-arrested and hybrid-released translation as encoding for an 18-kda hsp. th ... | 1993 | 8112573 |
| isolation and characterization of cdnas encoding wheat 3-hydroxy-3-methylglutaryl coenzyme a reductase. | the enzyme 3-hydroxy-3-methylglutaryl coenzyme a reductase (hmgr, ec 1.1.1.34) is a key enzyme in the isoprenoid biosynthetic pathway. we have isolated partial cdnas from wheat (triticum aestivum) using the polymerase chain reaction. comparison of deduced amino acid sequences of these cdnas shows that they represent a small family of genes that share a high degree of sequence homology among themselves as well as among genes from other organisms including tomato, arabidopsis, hamster, human, dros ... | 1993 | 8108513 |
| a dna-polymerase-related reading frame (pol-r) in the mtdna of secale cereale. | mitochondrial (mt)dna of secale cereale contains an open reading frame (pol-r), the potential translation product of which shows significant homology to the type-b dna polymerase encoded by the s1 plasmid of zea mays; it contains the highly-conserved domains iia to v of family b polymerases. the pol-r orf is transcribed, as proven by rt-pcr, but the transcript is not edited. upstream of the putative start codon a potential promoter motif was detected, fitting well into the postulated consensus s ... | 1994 | 8082167 |
| high-resolution cytological mapping of the long arm of chromosome 5a in common wheat using a series of deletion lines induced by gametocidal (gc) genes of aegilops speltoides. | gametocidal (gc) genes of aegilops in the background of the wheat genome lead to breakage of wheat chromosomes. the q gene of wheat was used as a marker to select 19 deletion lines for the long arm of chromosome 5a of common wheat, triticum aestivum cv. chinese spring (cs). the extents of deleted segments were cytologically estimated by the c-banding technique. the dnas of deletion lines were hybridized with 22 dna probes recognizing sites on the long arm of the chromosome (5al) to determine the ... | 1994 | 8058036 |
| a wheat (triticum aestivum) cdna clone encoding a plastid-localized heat-shock protein. | 1993 | 8029345 | |
| purification, characterization and cdna cloning of the 200 kda protein induced by cold acclimation in wheat. | we have purified to homogeneity the 200 kda protein induced specifically by low temperature in wheat (triticum aestivum l.). the boiling solubility of the protein has been used as a main step in the purification procedure. amino acid composition indicates that the 200 kda has a compositional bias for glycine (11.4%), threonine (13.3%), and alanine (22.0%). using oligonucleotide probes, we have isolated a clone (p wcs200) from a cold-acclimated winter wheat cdna library. northern analysis demonst ... | 1993 | 8025821 |
| five genes induced by aluminum in wheat (triticum aestivum l.) roots. | five different cdnas (termed wali1 to wali5 for wheat aluminum induced) whose expression was induced by al stress have been isolated from the root tips of al-treated wheat (triticum aestivum l.) plants. four of these genes were induced 24 to 96 h after al treatment, and their expression is reduced when the al is removed. each of these four genes was induced by inhibitory levels of al in two wheat cultivars--warigal, an al-sensitive cultivar, and waalt, an al-tolerant cultivar. the fifth gene (wa ... | 1993 | 8022939 |
| chromosome painting in plants: in situ hybridization with a dna probe from a specific microdissected chromosome arm of common wheat. | we report here on the successful painting of a specific plant chromosome within its own genome. isochromosomes for the long arm of chromosome 5 of the wheat b genome (5bl) were microdissected from first meiotic metaphase spreads of a monoisosomic 5bl line of the common wheat triticum aestivum cv. chinese spring. the dissected isochromosomes were amplified by degenerate oligonucleotide-primed pcr in a single tube reaction. the amplified dna was used as a complex probe mixture for fluorescent in s ... | 1994 | 7991581 |
| wali6 and wali7. genes induced by aluminum in wheat (triticum aestivum l.) roots. | 1994 | 7972509 | |
| shelf life of weaning foods developed from locally available food stuffs. | four weaning foods were formulated using locally available cereals and pulses such as wheat (triticum aestivum), barley (hordeum vulgare), green gram (vigna radiata) and jaggery. cereal, pulse and jaggery were used in the proportion of 70:30:25. roasting and malting were two processing techniques used. the developed weaning foods were evaluated for their nutritional characteristics and shelf life. all the formulations had a nutrient composition within the range prescribed by the indian standard ... | 1994 | 7971776 |
| hormonal and environmental responsiveness of a developmentally regulated protein repair l-isoaspartyl methyltransferase in wheat. | the l-isoaspartyl protein methyltransferase (ec 2.1.1.77) has been proposed to be involved in the repair of spontaneously damaged proteins by facilitating the conversion of abnormal l-isoaspartyl residues to normal l-aspartyl residues. based on the abundance of this enzyme in the seeds of a variety of plants and its unique substrate specificity, it has been hypothesized that it functions to prevent the accumulation of abnormal aspartyl residues in the proteins of aging seeds that can limit the v ... | 1994 | 7929264 |
| new 18s.26s ribosomal rna gene loci: chromosomal landmarks for the evolution of polyploid wheats. | three new 18s.26s rrna gene loci were identified in common wheat by sequential n-banding and in situ hybridization (ish) analysis. locus nor-a7 is located at the terminal area of the long arm of 5a in both diploid and polyploid wheats. locus nor-b6 is located in n-band 1bl2.5 of the long arm of chromosome 1b in triticum turgidum and triticum aestivum. ish sites, similar to nor-b6, were also detected on the long arms of chromosomes 1g in triticum timopheevii and 1s in aegilops speltoides, but the ... | 1994 | 7924620 |
| molecular variation in chloroplast dna regions in ancestral species of wheat. | restriction map variation in two 5-6-kb chloroplast dna regions of five diploid aegilops species in the section sitopsis and two wild tetraploid wheats, triticum dicoccoides and triticum araraticum, was investigated with a battery of four-cutter restriction enzymes. a single accession each of triticum durum, triticum timopheevi and triticum aestivum was included as a reference. more than 250 restriction sites were scored, of which only seven sites were found polymorphic in aegilops speltoides. n ... | 1994 | 7916310 |
| wheat acetyl-coenzyme a carboxylase: cdna and protein structure. | cdna fragments encoding part of wheat (triticum aestivum) acetyl-coa carboxylase (acc; ec 6.4.1.2) were cloned by pcr using primers based on the alignment of several biotin-dependent carboxylases. a set of overlapping clones encoding the entire wheat acc was then isolated by using these fragments as probes. the cdna sequence contains a 2257-amino acid reading frame encoding a 251-kda polypeptide. the amino acid sequence of the most highly conserved domain, corresponding to the biotin carboxylase ... | 1994 | 7913745 |
| immunohistochemical investigations on the pyloric glands of the ruin lizard (podarcis sicula campestris de betta). | mucous cells and enteroendocrine cells of the pyloric region of the ruin lizard (podarcis sicula campestris de betta) have been examined by lectin histochemical and immunohistochemical methods. binding to five plant lectins (canavalia ensiformis, con a; triticum vulgare, wheat germ, wgl; lotus tetragonolobus, winged pea, wpl; glycine max, soybean, sbl; arachis hypogaea, peanut, pnl) was performed to characterize glycoconjugates in the secretory products of superficial and glandular mucous cells. ... | 1994 | 7913280 |
| reduction by fluoranthene of copper and lead accumulation in triticum aestivum l. | 1994 | 7881214 | |
| [effect of addition of instant corn flour on rheological characteristics of wheat flour and breadmaking iii]. | the instant corn flour prepared by the hydrothermal process using corn grits soaked in water at room temperature (28-30 degrees c) for 5 hours and steaming for 1 minute at 118 degrees c presented characteristics similar to that of flours prepared with grits soaked in water at temperature higher than room temperature and different steaming time (5 and 15 minutes). the addition of instant corn flour up of a 25% mixture with wheat flour reduced the peak of maximum viscosity during the heating cycle ... | 1993 | 7872835 |
| high-efficiency plant regeneration from an embryogenic cell suspension culture of winter wheat (triticum aestivum l.). | highly embryogenic cell suspension cultures were established from immature embryo-derived embryogenic calli of winter wheat (triticum aestivum l., cv. gk ságvári). weekly subcultures were made in liquid ms medium supplemented with 2 mg 1(-1) 2,4-d. an average of twenty-two compact, organized calli were obtained from each 1 ml suspension cells when plated on solid ms medium containing iaa and zeatin under a 16/8 h light/dark cycle, while only 9 calli were produced in the dark. variation in the ca ... | 1993 | 7871929 |
| agglutinating activity of wheat gliadin peptide fractions in coeliac disease. | the k 562 (s) cell agglutinating activity of peptides obtained from in vitro digestion of bread wheat gliadins has been shown to be associated with a small fraction (coded as fraction c), that can be easily separated by affinity chromatography of the whole digest on a sepharose 6-b-mannan or sepharose 6-b-oligomers of n-acetyl-glucosamine. although the whole gliadin digests from 12 durum wheat varieties were unable to agglutinate k 562 (s) cells, all these digests were found to contain an active ... | 1995 | 7863509 |
| abundance, variability and chromosomal location of microsatellites in wheat. | the potential of microsatellite sequences as genetic markers in hexaploid wheat (triticum aestivum) was investigated with respect to their abundance, variability, chromosomal location and usefulness in related species. by screening a lambda phage library, the total number of (ga)n blocks was estimated to be 3.6 x 10(4) and the number of (gt)n blocks to be 2.3 x 10(4) per haploid wheat genome. this results in an average distance of approximately 270 kb between these two microsatellite types combi ... | 1995 | 7854317 |
| comparison of genetic and physical maps of group 7 chromosomes from triticum aestivum l. | we present a high density physical map of homoeologous group 7 chromosomes from triticum aestivum l. using a series of 54 deletion lines, 6 random amplified polymorphic dna (rapd) markers and 91 cdna or genomic dna clones from wheat, barley and oat. so far, 51 chromosome segments have been distinguished by molecular markers, and 54 homoeoloci have been allocated among chromosomes 7a, 7b and 7d. the linear order of molecular markers along the chromosomes is almost identical in the a- b- and d-gen ... | 1994 | 7808416 |
| use of lectins to evaluate the effects of gaas softlaser on dog tendon. | 1. lectins labeled with colloidal gold particles were used for the ultrastructural evaluation of the biological effects of gaas softlaser irradiation on the healing of dog tendon wounds. 2. six dogs were submitted to tenotomy and tenorrhaphy on the right and left hind legs. all animals received laser irradiation (4 j/cm2) daily for ten days only on the left leg, and the right leg of the same animal was used as control. biopsies were taken 11, 22 and 40 days after surgery. 3. laser-irradiated and ... | 1994 | 7787808 |
| purification, characterization, and intracellular localization of glycosylated protein disulfide isomerase from wheat grains. | wheat (triticum aestivum) storage proteins fold and assemble into complexes that are linked by intra- and intermolecular disulfide bonds, but it is not yet clear whether these processes are spontaneous or require the assistance of endoplasmic reticulum (er)-resident enzymes and molecular chaperones. aiming to unravel these processes, we have purified and characterized the enzyme protein disulfide isomerase (pdi) from wheat endosperm, as well as studied its developmental expression and intracellu ... | 1995 | 7784507 |
| the developmental stage of inactivation of rye origin rrna genes in the embryo and endosperm of wheat x rye f1 hybrids. | to identify the developmental stage during which the preferential inactivation of rrna genes from the rye parent occurs in wheat x rye hybrids, nucleolar activity was evaluated in the embryo and endosperm of developing seeds of the hybrids. the hybrids were obtained from crosses of euploid and aneuploid lines of hexaploid wheat, triticum aestivum cv. chinese spring, with rye, secale cereale cv. centeio do alto. the number of nucleolar organizing regions (nors) and nucleoli present in the embryo ... | 1995 | 7780660 |
| histochemical study of lectin binding in the human fetal minor salivary glands. | the emerging synthesis of glycoconjugates containing specific oligosaccharides in developing human fetal labial and lingual salivary glands has been investigated by lectin histochemistry. an avidin-biotin technique was used to study the binding of lectins from ulex europeus i (uea-i), dolichos biflorus (dba), glycine maximus (sba), helix pomatia (hpa), arachis hypogaea (pna) and triticum vulgare (wga) to specific sugars on sections of tissue from labial glands, glands of blandin and nuhn, glands ... | 1995 | 7776265 |
| molecular features and mitochondrial import pathway of the 14-kilodalton subunit of cytochrome c reductase from potato. | the cytochrome c reductase complexes from fungi and mammals both contain a 14-kd protein (yeast, 14.4 kd; bovine, 13.4 kd) that does not directly participate in electron transfer but possibly is indirectly involved in the function of the complex and has a role in assembly of the multimeric enzyme. a subunit of comparable size was identified for the bc1 complex of higher plants. the 14-kd protein from potato (solanum tuberosum) was specifically separated from the isolated protein complex in the p ... | 1995 | 7770525 |