Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| a pilot study on post-evisceration contamination of broiler carcasses and ready-to-sell livers and intestines (mala) with campylobacter jejuni and campylobacter coli in a high-throughput south african poultry abattoir. | to assess post-evisceration contamination of broiler carcasses, 300 samples were randomly selected during routine slaughter in the winter of 2004. the samples originated from 50 chicken carcasses, taken directly after evisceration, as well as 25 samples from ready-to-sell packages of fresh intestines (mala) and livers. the samples were taken in batches over a period of 4 weeks to allow randomised sampling from different farms of origin. conventional culture-based detection methods of campylobact ... | 2006 | 17137050 |
| genotyping of campylobacter jejuni from broiler carcasses and slaughterhouse environment by amplified fragment length polymorphism. | we examined the occurrence and diversity of campylobacter jejuni on broiler carcasses during slaughter of an infected flock and in the slaughterhouse environment during slaughter and postdisinfection before a new production run. during the slaughter of a known c. jejuni infected broiler flock, samples were taken from broiler carcasses at 7 different stages during the process. thirty-seven sites in the slaughterhouse environment were sampled both during process and postdisinfection. the samples w ... | 2006 | 17135687 |
| study on the prevalence of campylobacter spp. from chicken meat in hanoi, vietnam. | campylobacter spp. is considered to be the most common bacterial cause of human gastroenteritis worldwide. in developing countries, campylobacter spp. diarrhea is an important cause of childhood morbidity. chicken meat is known to be a major source of campylobacteriosis infection in the world. the purpose of this study was to investigate the prevalence of campylobacter spp. in chicken meat. a total of 100 samples from breast part of chicken carcass were collected from retail market in hanoi. the ... | 2006 | 17135525 |
| study of salmonella, campylobacter, and escherichia coli contamination in raw food available in factories, schools, and hospital canteens in hanoi, vietnam. | this study on the contamination rates of raw foods available in factory, school, and hospital canteens in hanoi, vietnam, with the bacteria of salmonella, campylobacter, and escherichia coli (e. coli) was carried out between 2003 and 2004. a total of 177 raw food samples of vegetables, meat (beef and pork), fish, and poultry were examined to provide baseline data for evaluation of microbiological risks in general, and identification of potential vehicles for pathogenic cross-contamination in can ... | 2006 | 17135522 |
| conjugative transfer of chromosomally encoded antibiotic resistance from helicobacter pylori to campylobacter jejuni. | many strains of helicobacter pylori are naturally competent for transformation and able to transfer chromosomal dna among different isolates using a conjugation-like mechanism. in this study, we sought to determine whether h. pylori can transfer dna into campylobacter jejuni, a closely related species of the campylobacterales group. to monitor the transfer, a chromosomally encoded streptomycin resistance cassette prearranged by a specific mutation in the rpsl gene of h. pylori was used. mating o ... | 2007 | 17135441 |
| temperate bacteriophages affect pulsed-field gel electrophoresis patterns of campylobacter jejuni. | the recently sequenced genome of campylobacter jejuni rm1221 revealed the presence of three integrated bacteriophage-like elements. in this study, genes from the first element, a mu-like bacteriophage, were amplified by pcr and used to probe pulsed-field gels of clinical c. jejuni strains obtained from a waterborne outbreak (ontario, canada, 2000). these highly similar strains differed only by their pulsed-field gel electrophoresis (pfge) patterns due to an apparent insertion or deletion of a 40 ... | 2007 | 17135440 |
| a multi-centre prospective case-control study of campylobacter infection in persons aged 5 years and older in australia. | there are an estimated 277,000 cases of campylobacteriosis in australia each year, most of which are thought to be sporadically acquired. to explore causes for these infections, we conducted a multi-centre case-control study of patients and community controls across five australian states during 2001-2002. a total of 881 campylobacter cases and 833 controls aged 5 years were recruited into the study. crude logistic analyses were conducted within various food and non-food exposure groups. a final ... | 2007 | 17134530 |
| a multi-centre prospective case-control study of campylobacter infection in persons aged 5 years and older in australia. | there are an estimated 277,000 cases of campylobacteriosis in australia each year, most of which are thought to be sporadically acquired. to explore causes for these infections, we conducted a multi-centre case-control study of patients and community controls across five australian states during 2001-2002. a total of 881 campylobacter cases and 833 controls aged 5 years were recruited into the study. crude logistic analyses were conducted within various food and non-food exposure groups. a final ... | 2007 | 17134530 |
| morphological and physiological responses of campylobacter jejuni to stress. | under conditions of stress, cells of campylobacter assume a coccoid shape that may be an evolutionary strategy evolved by the organism to enable survival between hosts. however, the physiology of campylobacter as it devolves from spiral to coccoid-shaped morphology is poorly understood. in this study, conditions influencing the survival of campylobacter jejuni atcc 35921 in broth were determined. cells in late log phase were resuspended in broth at 4 or 60 degrees c. the culturability of these c ... | 2006 | 17133821 |
| a comparative study of two food model systems to test the survival of campylobacter jejuni at -18 degrees c. | the survival of campylobacter jejuni nctc 11168 was tested at freezing conditions (-18 degrees c) over a period of 32 days in two food models that simulated either (i) the chicken skin surface (skin model) or (ii) the chicken juice in and around a broiler carcass (liquid model). in the skin model, cells were suspended in chicken juice or brain heart infusion broth (bhib) and added to 4-cm2 skin pieces, which were subsequently stored at -18 degrees c. in the liquid model, cells were suspended in ... | 2006 | 17133806 |
| defensins and cathelicidins in gastrointestinal infections. | to review recently published studies presenting novel and relevant information on antimicrobial peptides in gastrointestinal infections. | 2007 | 17133082 |
| relationship of bacterial strains to clinical syndromes of campylobacter-associated neuropathies. | clinical and serologic studies suggest that guillain-barré syndrome (gbs) and atypical gbs with preserved muscle stretch reflexes (msrs) form a continuous spectrum as well as do fisher syndrome (fs), fs/gbs overlap, bickerstaff brainstem encephalitis (bbe), bbe/gbs overlap, acute ophthalmoparesis (ao), ataxic gbs, and acute oropharyngeal palsy. | 2006 | 17130419 |
| c57bl/6 and congenic interleukin-10-deficient mice can serve as models of campylobacter jejuni colonization and enteritis. | campylobacter jejuni is a globally distributed cause of human food-borne enteritis and has been linked to chronic joint and neurological diseases. we hypothesized that c. jejuni 11168 colonizes the gastrointestinal tract of both c57bl/6 mice and congenic c57bl/6 interleukin-10-deficient (il-10(-/-)) mice and that c57bl/6 il-10(-/-) mice experience c. jejuni 11168-mediated clinical signs and pathology. individually housed mice were challenged orally with c. jejuni 11168, and the course of infecti ... | 2007 | 17130251 |
| towards a portable microchip system with integrated thermal control and polymer waveguides for real-time pcr. | a novel real-time pcr microchip platform with integrated thermal system and polymer waveguides has been developed. the integrated polymer optical system for real-time monitoring of pcr was fabricated in the same su-8 layer as the pcr chamber, without additional masking steps. two suitable dna binding dyes, sytox orange and to-pro-3, were selected and tested for the real-time pcr processes. as a model, cadf gene of campylobacter jejuni has been amplified on the microchip. using the integrated opt ... | 2006 | 17124710 |
| genes and environment in irritable bowel syndrome: one step forward. | 2006 | 17124153 | |
| a microbial diagnostic microarray technique for the sensitive detection and identification of pathogenic bacteria in a background of nonpathogens. | a major challenge in microbial diagnostics is the parallel detection and identification of low-bundance pathogens within a complex microbial community. in addition, a high specificity providing robust, reliable identification at least at the species level is required. a microbial diagnostic microarray approach, using single nucleotide extension labeling with gyrb as the marker gene, was developed. we present a novel concept applying competitive oligonucleotide probes to improve the specificity o ... | 2007 | 17123456 |
| asymmetric guillain-barré syndrome. | guillain-barré syndrome (gbs) is a heterogeneous disorder according to clinical, electrophysiological, immunologic and pathologic findings. it has usually been considered as an immune-mediated polyneuropathy clinically characterised by acute symmetric muscle weakness and areflexia. we describe a patient who, after a campylobacter jejuni infection, developed an acute motor-sensory neuropathy with marked and persistent asymmetry of clinical and electrophysiological findings. he had a high titre of ... | 2006 | 17122947 |
| operon prediction for sequenced bacterial genomes without experimental information. | various computational approaches have been proposed for operon prediction, but most algorithms rely on experimental or functional data that are only available for a small subset of sequenced genomes. in this study, we explored the possibility of using phylogenetic information to aid in operon prediction, and we constructed a bayesian hidden markov model that incorporates comparative genomic data with traditional predictors, such as intergenic distances. the prediction algorithm performs as well ... | 2007 | 17122389 |
| operon prediction for sequenced bacterial genomes without experimental information. | various computational approaches have been proposed for operon prediction, but most algorithms rely on experimental or functional data that are only available for a small subset of sequenced genomes. in this study, we explored the possibility of using phylogenetic information to aid in operon prediction, and we constructed a bayesian hidden markov model that incorporates comparative genomic data with traditional predictors, such as intergenic distances. the prediction algorithm performs as well ... | 2007 | 17122389 |
| comparison of antimicrobial susceptibility testing of campylobacter spp. by the agar dilution and the agar disk diffusion methods. | the correlation and the level of agreement between the standardized agar dilution and the agar disk diffusion methods for antimicrobial susceptibility testing of campylobacter were investigated. a high-level agreement between the two methods was evident for aminoglycosides and fluoroquinolones, while a low-level agreement was observed for other antibiotics. | 2007 | 17122005 |
| comparison of antimicrobial susceptibility testing of campylobacter spp. by the agar dilution and the agar disk diffusion methods. | the correlation and the level of agreement between the standardized agar dilution and the agar disk diffusion methods for antimicrobial susceptibility testing of campylobacter were investigated. a high-level agreement between the two methods was evident for aminoglycosides and fluoroquinolones, while a low-level agreement was observed for other antibiotics. | 2007 | 17122005 |
| multilocus sequence typing method for identification and genotypic classification of pathogenic leptospira species. | leptospira are the parasitic bacterial organisms associated with a broad range of mammalian hosts and are responsible for severe cases of human leptospirosis. the epidemiology of leptospirosis is complex and dynamic. multiple serovars have been identified, each adapted to one or more animal hosts. adaptation is a dynamic process that changes the spatial and temporal distribution of serovars and clinical manifestations in different hosts. serotyping based on repertoire of surface antigens is an a ... | 2006 | 17121682 |
| how flagellin and toll-like receptor 5 contribute to enteric infection. | 2006 | 17118981 | |
| how flagellin and toll-like receptor 5 contribute to enteric infection. | 2006 | 17118981 | |
| influence of dangling ends and surface-proximal tails of targets on probe-target duplex formation in 16s rrna gene-based diagnostic arrays. | dangling ends and surface-proximal tails of gene targets influence probe-target duplex formation and affect the signal intensity of probes on diagnostic microarrays. this phenomenon was evaluated using an oligonucleotide microarray containing 18-mer probes corresponding to the 16s rrna genes of 10 waterborne pathogens and a number of synthetic and pcr-amplified gene targets. signal intensities for klenow/random primer-labeled 16s rrna gene targets were dissimilar from those for 45-mer synthetic ... | 2006 | 17114322 |
| influence of dangling ends and surface-proximal tails of targets on probe-target duplex formation in 16s rrna gene-based diagnostic arrays. | dangling ends and surface-proximal tails of gene targets influence probe-target duplex formation and affect the signal intensity of probes on diagnostic microarrays. this phenomenon was evaluated using an oligonucleotide microarray containing 18-mer probes corresponding to the 16s rrna genes of 10 waterborne pathogens and a number of synthetic and pcr-amplified gene targets. signal intensities for klenow/random primer-labeled 16s rrna gene targets were dissimilar from those for 45-mer synthetic ... | 2006 | 17114322 |
| the first database comprised of flagellin gene (flaa) types of campylobacter jejuni human clinical isolates from greece. | flagellin subunit a gene (flaa) typing of campylobacter has been recognized by several groups as a relatively simple and quick genotyping method. the present study aimed to create, for the first time in greece, a database with flaa restriction patterns, which could be used for future epidemiological and clinical studies. a total of 207 c. jejuni clinical isolates of known serotype were collected from 5 general hospitals of the area of attica, during the period 2000-2003. | 2006 | 17111250 |
| specific detection of campylobacter jejuni from faeces using single nucleotide polymorphisms. | specimens of human faeces were tested by a rapid strategy for detection of campylobacter jejuni lineages by the presence of specific single nucleotide polymorphisms (snps) based on the c. jejuni multi locus sequence typing (mlst) scheme. this strategy was derived from analysis of the mlst databases to identify clonal complex specific snps followed by the design of real-time pcr assays to enable identification of six major c. jejuni clonal complexes associated with cases of human infection. the o ... | 2007 | 17109769 |
| evaluation of the role of h pylori infection in pathogenesis of gastric cancer by immunoblot assay. | to elucidate the different serological reactions to h pylori using the immunoblotting technique for further understanding of its pathogenic role in gastric cancer. | 2006 | 17109500 |
| detection of coccidioides species in clinical specimens by real-time pcr. | coccidioides spp. are dimorphic fungal pathogens endemic to the semiarid regions of north, central, and south america. currently, direct smear and culture are the most common means of identifying coccidioides spp. while these methods offer relatively sensitive and specific means of detecting coccidioides spp., growth in culture may take up to 3 weeks, potentially delaying the diagnosis and initiation of appropriate antifungal therapy. in addition, growth of the organism represents a significant ... | 2006 | 17108077 |
| detection of coccidioides species in clinical specimens by real-time pcr. | coccidioides spp. are dimorphic fungal pathogens endemic to the semiarid regions of north, central, and south america. currently, direct smear and culture are the most common means of identifying coccidioides spp. while these methods offer relatively sensitive and specific means of detecting coccidioides spp., growth in culture may take up to 3 weeks, potentially delaying the diagnosis and initiation of appropriate antifungal therapy. in addition, growth of the organism represents a significant ... | 2006 | 17108077 |
| utility of serum campylobacter specific antibodies in determining prior campylobacter infection in neurological disease. | campylobacter jejuni has been implicated in the pathogenesis of guillain-barre syndrome (gbs); however, little information exists on the utility of campylobacter serology in determining recent infection in the patient population. c. jejuni specific antibodies (cas) were measured in 420 blood donors (controls), 99 patients with recent c. jejuni infection, 34 patients with central nervous system disorders (neurology controls), and 44 patients with peripheral nervous system (pns) disorders: 18 with ... | 2007 | 17107803 |
| the effect of lemon, orange and bergamot essential oils and their components on the survival of campylobacter jejuni, escherichia coli o157, listeria monocytogenes, bacillus cereus and staphylococcus aureus in vitro and in food systems. | to investigate the effectiveness of oils and vapours of lemon (citrus limon), sweet orange (citrus sinensis) and bergamot (citrus bergamia) and their components against a number of common foodborne pathogens. | 2006 | 17105553 |
| the genome of deep-sea vent chemolithoautotroph thiomicrospira crunogena xcl-2. | presented here is the complete genome sequence of thiomicrospira crunogena xcl-2, representative of ubiquitous chemolithoautotrophic sulfur-oxidizing bacteria isolated from deep-sea hydrothermal vents. this gammaproteobacterium has a single chromosome (2,427,734 base pairs), and its genome illustrates many of the adaptations that have enabled it to thrive at vents globally. it has 14 methyl-accepting chemotaxis protein genes, including four that may assist in positioning it in the redoxcline. a ... | 2006 | 17105352 |
| identification of the functional initiation codons of a phase-variable gene of haemophilus influenzae, lic2a, with the potential for differential expression. | simple sequence repeats located within reading frames mediate phase-variable on/off switches in gene expression by generating frameshifts. multiple translation initiation codons in different reading frames are found upstream of most haemophilus influenzae tetranucleotide repeat tracts, raising the possibility of multiple active reading frames and more than two levels of gene expression for these loci. phase variation between three levels of gene expression (strong, weak, and none) was observed w ... | 2007 | 17098909 |
| identification of the functional initiation codons of a phase-variable gene of haemophilus influenzae, lic2a, with the potential for differential expression. | simple sequence repeats located within reading frames mediate phase-variable on/off switches in gene expression by generating frameshifts. multiple translation initiation codons in different reading frames are found upstream of most haemophilus influenzae tetranucleotide repeat tracts, raising the possibility of multiple active reading frames and more than two levels of gene expression for these loci. phase variation between three levels of gene expression (strong, weak, and none) was observed w ... | 2007 | 17098909 |
| role of gne and gale in the virulence of aeromonas hydrophila serotype o34. | the mesophilic aeromonas hydrophila ah-3 (serotype o34) strain shows two different udp-hexose epimerases in its genome: gale (ec 3.1.5.2) and gne (ec 3.1.5.7). similar homologues were detected in the different mesophilic aeromonas strains tested. gale shows only udp-galactose 4-epimerase activity, while gne is able to perform a dual activity (mainly udp-n-acetyl galactosamine 4-epimerase and also udp-galactose 4-epimerase). we studied the activities in vitro of both epimerases and also in vivo t ... | 2007 | 17098903 |
| role of gne and gale in the virulence of aeromonas hydrophila serotype o34. | the mesophilic aeromonas hydrophila ah-3 (serotype o34) strain shows two different udp-hexose epimerases in its genome: gale (ec 3.1.5.2) and gne (ec 3.1.5.7). similar homologues were detected in the different mesophilic aeromonas strains tested. gale shows only udp-galactose 4-epimerase activity, while gne is able to perform a dual activity (mainly udp-n-acetyl galactosamine 4-epimerase and also udp-galactose 4-epimerase). we studied the activities in vitro of both epimerases and also in vivo t ... | 2007 | 17098903 |
| dynamic state of dna topology is essential for genome condensation in bacteria. | in bacteria, dps is one of the critical proteins to build up a condensed nucleoid in response to the environmental stresses. in this study, we found that the expression of dps and the nucleoid condensation was not simply correlated in escherichia coli, and that fis, which is an e. coli (gamma-proteobacteria)-specific nucleoid protein, interfered with the dps-dependent nucleoid condensation. atomic force microscopy and northern blot analyses indicated that the inhibitory effect of fis was due to ... | 2006 | 17093499 |
| clonal complexes of campylobacter jejuni identified by multilocus sequence typing are reliably predicted by restriction fragment length polymorphism analyses of the flaa gene. | multilocus sequence typing (mlst) has provided important new insights into the population structure of campylobacter jejuni and is rapidly becoming the gold standard for typing this species. however, the methodology is comparatively costly and slow to perform for the routine surveillance testing of large numbers of isolates required by public health laboratories. restriction fragment length polymorphism analysis of the flaa gene (rflp-flaa) and sequencing of the variable region in the fla locus ... | 2007 | 17093018 |
| use of a combination of brushing technique and the loop-mediated isothermal amplification method as a novel, rapid, and safe system for detection of helicobacter pylori. | gastric mucosal biopsy is widely used in the detection of helicobacter pylori but is associated with a number of problems, including false-negative results due to sampling error and massive bleeding after biopsy. given the extended period required to culture h. pylori, detection would be further improved by the use of rapid detection methods such as pcr. here, we developed a rapid, safe, and convenient method for collecting h. pylori which combines endoscopic brushing with the loop-mediated isot ... | 2006 | 17088368 |
| in vitro biosynthesis of udp-n,n'-diacetylbacillosamine by enzymes of the campylobacter jejuni general protein glycosylation system. | in campylobacter jejuni 2,4-diacetamido-2,4,6-trideoxy-alpha-d-glucopyranose, termed n,n'-diacetylbacillosamine (bac2,4dinac), is the first carbohydrate in the glycoprotein n-linked heptasaccharide. with uridine diphosphate-n-acetylglucosamine (udp-glcnac) as a starting point, two enzymes of the general protein glycosylation (pgl) pathway in c. jejuni (pglf and pgle) have recently been shown to modify this sugar nucleotide to form udp-2-acetamido-4-amino-2,4,6-trideoxy-alpha-d-glycopyranose (udp ... | 2006 | 17087520 |
| characterization of macrolide-resistant campylobacter coli isolates from food-producing animals on farms across japan during 2004. | we investigated the susceptibilities against 7 antimicrobial agents in campylobacter jejuni and c. coli isolates from food-producing animals in 2004. in comparison with the results of past surveillance, no significant difference was observed in resistance rates against all of the antimicrobials tested in campylobacter isolates. however, slight increase of erythromycin (em) resistance was found in c. coli isolates from pigs. we examined the mutation of the 23s rrna gene and their susceptibilities ... | 2006 | 17085893 |
| autoinducer-2-producing protein luxs, a novel salt- and chloride-induced protein in the moderately halophilic bacterium halobacillus halophilus. | the moderately halophilic bacterium halobacillus halophilus carries a homologue of luxs, a protein involved in the activated methyl cycle and the production of autoinducer-2, which mediates quorum sensing between certain species. luxs of h. halophilus is part of an operon that encodes an s-adenosylmethionine-dependent methyltransferase, a cysteine synthase, and a beta-cystathionine lyase. expression of luxs was growth phase dependent, with maximal expression in the mid-exponential growth phase. ... | 2007 | 17085700 |
| autoinducer-2-producing protein luxs, a novel salt- and chloride-induced protein in the moderately halophilic bacterium halobacillus halophilus. | the moderately halophilic bacterium halobacillus halophilus carries a homologue of luxs, a protein involved in the activated methyl cycle and the production of autoinducer-2, which mediates quorum sensing between certain species. luxs of h. halophilus is part of an operon that encodes an s-adenosylmethionine-dependent methyltransferase, a cysteine synthase, and a beta-cystathionine lyase. expression of luxs was growth phase dependent, with maximal expression in the mid-exponential growth phase. ... | 2007 | 17085700 |
| longitudinal study of finnish campylobacter jejuni and c. coli isolates from humans, using multilocus sequence typing, including comparison with epidemiological data and isolates from poultry and cattle. | we describe a study on the application of multilocus sequence typing for the analysis of campylobacter jejuni and c. coli isolates from human domestically acquired infections in the helsinki-uusimaa area of finland in 1996, 2002, and 2003. in addition, isolates from poultry meat and fecal samples of cattle from the seasonal peak (july to september) in 2003 were included in the study. in total, 361 finnish c. jejuni and c. coli strains were typed. sequence type 45 (st-45) (45%), st-21 (21%), and ... | 2007 | 17085689 |
| evaluation of eight rna isolation methods for transcriptional analysis in campylobacter jejuni. | dna-free rna samples are essential to investigate gene regulation using real-time rt-pcr. this study evaluated eight rna isolation methods in combination with turbo dnase treatment and acid phenol extractions for their ability to produce dna-free rna from campylobacter jejuni strains. | 2007 | 17084472 |
| growth temperature of four campylobacter jejuni strains influences their subsequent survival in food and water. | to determine if campylobacter jejuni grown at 37 and 42 degrees c have different abilities to survive on beef and chicken, and in water. | 2006 | 17083703 |
| guillain-barré syndrome and its treatment. | guillain-barré syndrome typically presents with an acute ascending areflexic weakness, progressing over 4 weeks or less. the most common form of the disease is an acute inflammatory demyelinating polyneuropathy, but other forms with primarily axonal pathologies are well documented. the association of guillain-barré syndrome with a range of antecedent infections, particularly campylobacter jejuni enteritis, is also established. a range of serological and neurophysiological investigations can assi ... | 2006 | 17078795 |
| the immunologically distinct o antigens from francisella tularensis subspecies tularensis and francisella novicida are both virulence determinants and protective antigens. | we have determined the sequence of the gene cluster encoding the o antigen in francisella novicida and compared it to the previously reported o-antigen cluster in francisella tularensis subsp. tularensis. immunization with purified lipopolysaccharide (lps) from f. tularensis subsp. tularensis or f. novicida protected against challenge with francisella tularensis subsp. holarctica and f. novicida, respectively. the lps from f. tularensis subsp. tularensis did not confer protection against challen ... | 2007 | 17074846 |
| the immunologically distinct o antigens from francisella tularensis subspecies tularensis and francisella novicida are both virulence determinants and protective antigens. | we have determined the sequence of the gene cluster encoding the o antigen in francisella novicida and compared it to the previously reported o-antigen cluster in francisella tularensis subsp. tularensis. immunization with purified lipopolysaccharide (lps) from f. tularensis subsp. tularensis or f. novicida protected against challenge with francisella tularensis subsp. holarctica and f. novicida, respectively. the lps from f. tularensis subsp. tularensis did not confer protection against challen ... | 2007 | 17074846 |
| application of biochemical and polymerase chain reaction assays for identification of campylobacter isolates from non-human primates. | a multiplex polymerase chain reaction (pcr) assay was performed on 167 thermophilic campylobacters isolated from non-human primates. samples were first identified by phenotypic methods resulting in 64 campylobacter jejuni and 103 c. coli strains. four strains identified biochemically as c. coli, were then determined to be c. jejuni by pcr. comparison of methodologies showed that the main discrepancies were attributed to the hippurate hydrolysis test and sensitivity to cephalothin and nalidixic a ... | 2006 | 17072452 |
| demyelinating diseases. | a diagnosis of demyelination carries important therapeutic and prognostic implications. in most cases the diagnosis is made clinically, and involvement of the histopathologist is largely confined to postmortem confirmation and clinicopathological correlation. however, every now and then, accurate diagnosis of the presence or cause of demyelination before death hinges on the histopathological assessment. recognition of demyelination depends on an awareness of this as a diagnostic possibility, and ... | 2006 | 17071802 |
| sensitive quantitative detection of commensal bacteria by rrna-targeted reverse transcription-pcr. | a sensitive rrna-targeted reverse transcription-quantitative pcr (rt-qpcr) method was developed for exact and sensitive enumeration of subdominant bacterial populations. using group- or species-specific primers for 16s or 23s rrna, analytical curves were constructed for escherichia coli, enterococcus faecalis, staphylococcus aureus, clostridium perfringens, and pseudomonas aeruginosa, and the threshold cycle value was found to be linear up to an rna amount of 10(-3) cell per rt-pcr. the number o ... | 2007 | 17071791 |
| sensitive quantitative detection of commensal bacteria by rrna-targeted reverse transcription-pcr. | a sensitive rrna-targeted reverse transcription-quantitative pcr (rt-qpcr) method was developed for exact and sensitive enumeration of subdominant bacterial populations. using group- or species-specific primers for 16s or 23s rrna, analytical curves were constructed for escherichia coli, enterococcus faecalis, staphylococcus aureus, clostridium perfringens, and pseudomonas aeruginosa, and the threshold cycle value was found to be linear up to an rna amount of 10(-3) cell per rt-pcr. the number o ... | 2007 | 17071791 |
| divergent evolution of a structural proteome: phenomenological models. | we develop models of the divergent evolution of genomes; the elementary object of sequence dynamics is the protein structural domain. to identify patterns of organization that reflect mechanisms of evolution, we consider the individual genomes of many procaryote species, studying the arrangement of protein structural domains in the space of all polypeptide structures. we view the network of structural similarities as a graph, called the organismal protein domain universe graph (opdug); vertices ... | 2006 | 17071665 |
| divergent evolution of a structural proteome: phenomenological models. | we develop models of the divergent evolution of genomes; the elementary object of sequence dynamics is the protein structural domain. to identify patterns of organization that reflect mechanisms of evolution, we consider the individual genomes of many procaryote species, studying the arrangement of protein structural domains in the space of all polypeptide structures. we view the network of structural similarities as a graph, called the organismal protein domain universe graph (opdug); vertices ... | 2006 | 17071665 |
| a case of guillain-barrè syndrome following staphylococcus aureus endocarditis. | guillain-barrè syndrome (gbs) is the most important cause of acute neuromuscular paralysis in western countries and it is preceded in almost all cases by an infectious disease such as campylobacter jejuni or cytomegalovirus. however, gbs associated with previous bacterial endocarditis is very rare. we report the case of a 74-year-old man with gbs following staphylococcus aureus endocarditis affecting aortic valve. although the absolute incidence of gbs is low, the present case stresses the need ... | 2007 | 17070944 |
| synthesis of reference standards to enable single cell metabolomic studies of tetramethylrhodamine-labeled ganglioside gm1. | ganglioside gm1 and its seven potential catabolic products: asialo-gm1, gm2, asialo-gm2, gm3, lac-cer, glc-cer and cer, were labeled with tetramethylrhodamine (tmr) to permit ultra-sensitive analysis using laser-induced fluorescence (lif) detection. the preparation involved acylation of the homogenous c(18)lyso-forms of gm1, lac-cer, glc-cer and cer with the n-hydroxysuccinimide ester of a beta-alanine-tethered 6-tmr derivative, followed by conversion of these labeled products using galactosidas ... | 2007 | 17069778 |
| synthesis of reference standards to enable single cell metabolomic studies of tetramethylrhodamine-labeled ganglioside gm1. | ganglioside gm1 and its seven potential catabolic products: asialo-gm1, gm2, asialo-gm2, gm3, lac-cer, glc-cer and cer, were labeled with tetramethylrhodamine (tmr) to permit ultra-sensitive analysis using laser-induced fluorescence (lif) detection. the preparation involved acylation of the homogenous c(18)lyso-forms of gm1, lac-cer, glc-cer and cer with the n-hydroxysuccinimide ester of a beta-alanine-tethered 6-tmr derivative, followed by conversion of these labeled products using galactosidas ... | 2007 | 17069778 |
| an inventory of yeast proteins associated with nucleolar and ribosomal components. | although baker's yeast is a primary model organism for research on eukaryotic ribosome assembly and nucleoli, the list of its proteins that are functionally associated with nucleoli or ribosomes is still incomplete. we trained a naïve bayesian classifier to predict novel proteins that are associated with yeast nucleoli or ribosomes based on parts lists of nucleoli in model organisms and large-scale protein interaction data sets. phylogenetic profiling and gene expression analysis were carried ou ... | 2006 | 17067374 |
| cryptic plasmids isolated from campylobacter strains represent multiple, novel incompatibility groups. | three small, cryptic plasmids from the multi-drug-resistant (mdr) campylobacter coli strain rm2228 and one small, cryptic plasmid from the mdr campylobacter jejuni strain rm1170 were sequenced and characterized. pcc2228-1 has some similarity to firmicutes repl family plasmids that replicate via a rolling-circle mechanism. pcc2228-2 is a theta-replicating, iteron-containing plasmid (icp) that is a member of the same incompatibility (inc) group as previously described campylobacter shuttle vectors ... | 2007 | 17064774 |
| the yfgl lipoprotein is essential for type iii secretion system expression and virulence of salmonella enterica serovar enteritidis. | salmonella enterica, like many gram-negative pathogens, uses type three secretion systems (ttss) to infect its hosts. the three ttss of salmonella, namely, ttss-1, ttss-2, and flagella, play a major role in the virulence of this bacterium, allowing it to cross the intestinal barrier and to disseminate systemically. previous data from our laboratory have demonstrated the involvement of the chromosomal region harboring the yfgl, enga, and yfgj open reading frames in s. enterica serovar enteritidis ... | 2007 | 17060472 |
| the yfgl lipoprotein is essential for type iii secretion system expression and virulence of salmonella enterica serovar enteritidis. | salmonella enterica, like many gram-negative pathogens, uses type three secretion systems (ttss) to infect its hosts. the three ttss of salmonella, namely, ttss-1, ttss-2, and flagella, play a major role in the virulence of this bacterium, allowing it to cross the intestinal barrier and to disseminate systemically. previous data from our laboratory have demonstrated the involvement of the chromosomal region harboring the yfgl, enga, and yfgj open reading frames in s. enterica serovar enteritidis ... | 2007 | 17060472 |
| rapid separation and concentration of food-borne pathogens in food samples prior to quantification by viable-cell counting and real-time pcr. | buoyant density gradient centrifugation has been used to separate bacteria from complex food matrices, as well as to remove compounds that inhibit rapid detection methods, such as pcr, and to prevent false-positive results due to dna originating from dead cells. applying a principle of buoyant density gradient centrifugation, we developed a method for rapid separation and concentration following filtration and low- and high-speed centrifugation, as well as flotation and sedimentation buoyant den ... | 2007 | 17056684 |
| prevalence and levels of serum antibodies to gram negative microorganisms in turkish patients with hla-b27 positive acute anterior uveitis and controls. | acute anterior uveitis (aau), seronegative spondyloarthropathies such as ankylosing spondylitis and reactive arthritis form the group of "hla-b27-associated diseases." the aim of this study was to analyze the prevalence and levels of serum antibodies against gram negative bacteria in turkish patients with aau. | 2006 | 17056463 |
| identification and characterization of a cell surface protein of prevotella intermedia 17 with broad-spectrum binding activity for extracellular matrix proteins. | prevotella intermedia binds and invades a variety of host cells. this binding is most probably mediated through cell surface proteins termed adhesins. to identify proteins binding to the host extracellular matrix (ecm) component, fibronectin, and study the molecular mechanism underlying bacterial colonization, we applied proteomic approaches to perform a global investigation of p. intermedia strain 17 outer membrane proteins. 2-de followed by far western blot analysis using fibronectin as a prob ... | 2006 | 17051640 |
| helicobacter pylori flhb function: the flhb c-terminal homologue hp1575 acts as a "spare part" to permit flagellar export when the hp0770 flhbcc domain is deleted. | in helicobacter pylori 26695, a gene annotated hp1575 encodes a putative protein of unknown function which shows significant similarity to part of the c-terminal domain of the flagellar export protein flhb. in salmonella enterica, this part (flhb(cc)) is proteolytically cleaved from the full-length flhb, a processing event that is required for flagellar protein export and, thus, motility. the role of flhb (hp0770) and its c-terminal homologue hp1575 was studied in h. pylori using a range of nonp ... | 2006 | 17050924 |
| application of smartgene idns software to partial 16s rrna gene sequences for a diverse group of bacteria in a clinical laboratory. | laboratories often receive clinical isolates for bacterial identification that have ambiguous biochemical profiles by conventional testing. with the emergence of 16s rrna gene sequencing as an identification tool, we evaluated the usefulness of smartgene idns, a 16s rrna sequence database and software program for microbial identification. identification by conventional methods of a diverse group of bacterial clinical isolates was compared with gene sequences interrogated by the smartgene and mic ... | 2006 | 17050811 |
| outbreak of acute gastroenteritis in an air force base in western greece. | on the 20th september 2005, soldiers and staff at the air force base in western greece experienced an outbreak of acute gastroenteritis. the purpose of this study was to identify the agent and the source of the outbreak in order to develop control measures and to avoid similar outbreaks in the future. | 2006 | 17044937 |
| manndb - a microbial database of automated protein sequence analyses and evidence integration for protein characterization. | manndb was created to meet a need for rapid, comprehensive automated protein sequence analyses to support selection of proteins suitable as targets for driving the development of reagents for pathogen or protein toxin detection. because a large number of open-source tools were needed, it was necessary to produce a software system to scale the computations for whole-proteome analysis. thus, we built a fully automated system for executing software tools and for storage, integration, and display of ... | 2006 | 17044936 |
| cellulitis and septic arthritis caused by campylobacter fetus and campylobacter jejuni: report of 2 cases and review of the literature. | bacteremia caused by gram-negative bacteria occasionally causes soft tissue infections, including cellulitis and septic arthritis. we describe 1 patient each with campylobacter fetus cellulitis and septic arthritis and review the world literature with regard to c. fetus and c. jejuni infections at these sites. altogether, 14 patients with cellulitis (12 due to c. fetus and 2 due to c. jejuni) and 20 patients with septic arthritis (15 due to c. fetus and 4 caused by c. jejuni) have been described ... | 2003 | 17043445 |
| contribution of target gene mutations and efflux to decreased susceptibility of salmonella enterica serovar typhimurium to fluoroquinolones and other antimicrobials. | the mechanisms involved in fluoroquinolone resistance in salmonella enterica include target alterations and overexpression of efflux pumps. the present study evaluated the role of known and putative multidrug resistance efflux pumps and mutations in topoisomerase genes among laboratory-selected and naturally occurring fluoroquinolone-resistant salmonella enterica serovar typhimurium strains. strains with ciprofloxacin mics of 0.25, 4, 32, and 256 microg/ml were derived in vitro using serovar typ ... | 2007 | 17043131 |
| contribution of target gene mutations and efflux to decreased susceptibility of salmonella enterica serovar typhimurium to fluoroquinolones and other antimicrobials. | the mechanisms involved in fluoroquinolone resistance in salmonella enterica include target alterations and overexpression of efflux pumps. the present study evaluated the role of known and putative multidrug resistance efflux pumps and mutations in topoisomerase genes among laboratory-selected and naturally occurring fluoroquinolone-resistant salmonella enterica serovar typhimurium strains. strains with ciprofloxacin mics of 0.25, 4, 32, and 256 microg/ml were derived in vitro using serovar typ ... | 2007 | 17043131 |
| analysis of the roles of flgp and flgq in flagellar motility of campylobacter jejuni. | flagellar motility is an important determinant of campylobacter jejuni that is required for promoting interactions with various hosts to promote gastroenteritis in humans or commensal colonization of many animals. in a previous study, we identified a nonmotile mutant of c. jejuni 81-176 with a transposon insertion in cj1026c, but verification of the role of the encoded protein in motility was not determined. in this study, we have determined that cj1026c and the gene immediately downstream, cj10 ... | 2007 | 17041040 |
| campylobacter jejuni inactivation in new zealand soils. | the study was undertaken to determine the inactivation rate of campylobacter jejuni in new zealand soils. | 2006 | 17040243 |
| genetic variability of campylobacter jejuni isolated from fresh and frozen broiler carcasses. | the aim of this study was to determine the genetic variability of campylobacter jejuni isolates from poultry before and after freezing treatment in order to identify genotypes that would survive the treatment. | 2006 | 17040226 |
| natural presence of campylobacter spp. in various internal organs of commercial broiler breeder hens. | campylobacter are known to cause acute bacterial gastroenteritis in humans. poultry products have been implicated as a significant source of these infections. six experiments were performed to determine whether campylobacter could be isolated naturally from the primary and secondary lymphoid organs, liver/gallbladder, and ceca of commercial broiler breeder hens. broiler breeder hens were acquired from different commercial sources during the early, middle, and late lay cycles. the birds were euth ... | 2006 | 17039849 |
| presence of inoculated campylobacter and salmonella in unabsorbed yolks of male breeders raised as broilers. | day-old male broiler breeder chicks were obtained from a commercial hatchery and raised as broilers. for experiment 1, at 5 wk of age, the broilers were orally inoculated with a 10(6) cfu/ml of a characterized strain of campylobacter jejuni and a cocktail (three naladixic acid-resistant strains) of salmonella serovars. one week after inoculation, the birds were euthanatized and defeathered. the abdominal cavity was examined and any unabsorbed yolk material (and remaining yolk stalk) and ceca wer ... | 2006 | 17039845 |
| acute motor-sensory axonal neuropathy associated with active systemic lupus erythematosus and anticardiolipin antibodies. | acute motor-sensory axonal neuropathy (amsan) is an axonal variant of guillian-barré syndrome (gbs) that presents with acute ascending quadriparesis. this has generally been described in association with campylobacter jejuni infections or with anti-ganglioside antibodies. known cases have shown a slow recovery and a poor prognosis. we report a case with clinical and electrophysiological evidence of amsan in association with active systemic lupus erythematosus (sle) and anticardiolipin antibodies ... | 2001 | 17039164 |
| survival of campylobacter jejuni in potable water biofilms: a comparative study with different detection methods. | campylobacteria are important foodborne pathogens. c. jejuni bacteria have caused several drinking water-related epidemics in finland. normally, c. jejuni is not able to multiply in drinking water or in biofilms although it may survive in biofilms. the survival of c. jejuni in biofilms was studied using the propella biofilm reactor. the number of bacteria was analysed with traditional culture methods and with fluorescence in situ hybridisation (fish). by culture methods c. jejuni was detectable ... | 2006 | 17037133 |
| effect of oral administration of bismuth compounds on campylobacter colonization in broilers. | bismuth compounds have been used since the 18th century to treat gastrointestinal ailments in man. colloidal bismuth subcitrate (de-nol) is currently used in combination with antibiotics to reduce enteric helicobacter pylori colonization as a treatment of stomach ulcers. we investigated whether bismuth citrate or its parent compound, colloidal bismuth subcitrate, would reduce colonization of the closely related foodborne pathogen, campylobacter jejuni in chickens. in 2 studies, birds were either ... | 2006 | 17032837 |
| identification of labile udp-ketosugars in helicobacter pylori, campylobacter jejuni and pseudomonas aeruginosa: key metabolites used to make glycan virulence factors. | 2006 | 17031886 | |
| campylobacter infection: small bowel and colon. | the important contribution of campylobacter infections to human enteric disease is well established. recent completion of the genomic sequence of a campylobacter jejuni strain has heralded a renaissance in the field of campylobacter pathogenesis research. with the application of novel, powerful technologies, our understanding of how these organisms mediate disease is set to evolve rapidly from its current, relatively neglected status. | 2002 | 17031223 |
| high level of ciprofloxacin resistance and its molecular background among campylobacter jejuni strains isolated in the united arab emirates. | the antibiotic sensitivity and the serotype and molecular type (mt) distribution of 41 campylobacter jejuni strains isolated from individual patients in tawam hospital, al ain, united arab emirates, were investigated. while all strains were sensitive to erythromycin (mic 0.5-4 mg l(-1)), 35 isolates (85.4 %) exhibited resistance to ciprofloxacin (mic 8-64 mg l(-1)). all resistant strains carried the thr-86 to ile mutation in the gyrase a (gyra) gene, as shown by mismatch amplification mutation a ... | 2006 | 17030913 |
| modification of enrofloxacin treatment regimens for poultry experimentally infected with salmonella enterica serovar typhimurium dt104 to minimize selection of resistance. | we hypothesized that higher doses of fluoroquinolones for a shorter duration could maintain efficacy (as measured by reduction in bacterial count) while reducing selection in chickens of bacteria with reduced susceptibility. chicks were infected with salmonella enterica serovar typhimurium dt104 and treated 1 week later with enrofloxacin at the recommended dose for 5 days (water dose adjusted to give 10 mg/kg of body weight of birds or equivalence, i.e., water at 50 ppm) or at 2.5 or 5 times the ... | 2006 | 17030564 |
| [campylobacter jejuni and cytomegalovirus (cmv) infections in patients with the guillain-barre syndrome]. | guillain-barre syndrome (gbs) is a rare disease triggered by postinfectious mechanisms. the disease concerns all ages, and is widely distributed around the world. the principal risks are respiratory failure, especially during the initial phase of the disease, and persisting deficit at long term. among the infectious known agents, campylobacter jejuni and cmv represent more than 40% of gbs causes. the clinical presentation, and the long-term prognosis of gbs related to these two etiologies are di ... | 2006 | 17030119 |
| evidence for transmembrane proton transfer in a dihaem-containing membrane protein complex. | membrane protein complexes can support both the generation and utilisation of a transmembrane electrochemical proton potential ('proton-motive force'), either by transmembrane electron transfer coupled to protolytic reactions on opposite sides of the membrane or by transmembrane proton transfer. here we provide the first evidence that both of these mechanisms are combined in the case of a specific respiratory membrane protein complex, the dihaem-containing quinol:fumarate reductase (qfr) of woli ... | 2006 | 17024183 |
| bacterial infections of the small intestine and colon. | enterotoxigenic escherichia coli, vibrio cholerae o1, campylobacter jejuni, salmonella species, and shigella species are major causes of morbidity and death in diarrheal disease. more recently recognized pathogens are v. cholerae o139 and enterohemorrhagic e. coli. in addition to this, several presumptive virulence factors have been identified in diarrheagenic e. coli and in other species. to confirm these as virulence factors we need good diagnostic tools and good epidemiological studies. these ... | 1999 | 17023917 |
| role of the cmeabc efflux pump in the emergence of fluoroquinolone-resistant campylobacter under selection pressure. | the objective of this study was to determine the contribution of the cmeabc efflux pump to the emergence of fluoroquinolone (fq)-resistant mutants in campylobacter jejuni under various levels of selection pressure. | 2006 | 17023497 |
| structural determinants in the group iii truncated hemoglobin from campylobacter jejuni. | truncated hemoglobins (trhbs) constitute a distinct lineage in the globin superfamily, distantly related in size and fold to myoglobin and monomeric hemoglobins. their phylogenetic analyses revealed that three groups (i, ii, and iii) compose the trhb family. group i and ii trhbs adopt a simplified globin fold, essentially composed of a 2-on-2 alpha-helical sandwich, wrapped around the heme group. so far no structural data have been reported for group iii trhbs. here we report the three-dimension ... | 2006 | 17023416 |
| sub-speciating campylobacter jejuni by proteomic analysis of its protein biomarkers and their post-translational modifications. | we have identified several protein biomarkers of three campylobacter jejuni strains (rm1221, rm1859, and rm3782) by proteomic techniques. the protein biomarkers identified are prominently observed in the time-of-flight mass spectra (tof ms) of bacterial cell lysate supernatants ionized by matrix-assisted laser desorption/ionization (maldi). the protein biomarkers identified were: dna-binding protein hu, translation initiation factor if-1, cytochrome c553, a transthyretin-like periplasmic protein ... | 2006 | 17022624 |
| pleiotropic role of quorum-sensing autoinducer 2 in photorhabdus luminescens. | bacterial virulence is an integrative process that may involve quorum sensing. in this work, we compared by global expression profiling the wild-type entomopathogenic photorhabdus luminescens subsp. laumondii tt01 to a luxs-deficient mutant unable to synthesize the type 2 quorum-sensing inducer ai-2. ai-2 was shown to regulate more than 300 targets involved in most compartments and metabolic pathways of the cell. ai-2 is located high in the hierarchy, as it controls the expression of several tra ... | 2006 | 17021191 |
| novel method to identify source-associated phylogenetic clustering shows that listeria monocytogenes includes niche-adapted clonal groups with distinct ecological preferences. | while phylogenetic and cluster analyses are often used to define clonal groups within bacterial species, the identification of clonal groups that are associated with specific ecological niches or host species remains a challenge. we used listeria monocytogenes, which causes invasive disease in humans and different animal species and which can be isolated from a number of environments including food, as a model organism to develop and implement a two-step statistical approach to the identificatio ... | 2006 | 17021104 |
| detection and identification of human plasmodium species with real-time quantitative nucleic acid sequence-based amplification. | decisions concerning malaria treatment depend on species identification causing disease. microscopy is most frequently used, but at low parasitaemia (<20 parasites/mul) the technique becomes less sensitive and time consuming. rapid diagnostic tests based on plasmodium antigen detection do often not allow for species discrimination as microscopy does, but also become insensitive at <100 parasites/microl. | 2006 | 17018138 |
| disruption of tight junctions and induction of proinflammatory cytokine responses in colonic epithelial cells by campylobacter jejuni. | campylobacter jejuni is a leading cause of human enterocolitis and is associated with postinfectious complications, including irritable bowel syndrome and guillain-barré syndrome. however, the pathogenesis of c. jejuni infection remains poorly understood. paracellular pathways in intestinal epithelial cells are gated by intercellular junctions (tight junctions and adherens junctions), providing a functional barrier between luminal microbes and host immune cells in the lamina propria. here we des ... | 2006 | 17015453 |
| detection of prokaryotic promoters from the genomic distribution of hexanucleotide pairs. | in bacteria, sigma factors and other transcriptional regulatory proteins recognize dna patterns upstream of their target genes and interact with rna polymerase to control transcription. as a consequence of evolution, dna sequences recognized by transcription factors are thought to be enriched in intergenic regions (irs) and depleted from coding regions of prokaryotic genomes. | 2006 | 17014715 |
| survival of campylobacter spp. in darkling beetles (alphitobius diaperinus) and their larvae in australia. | campylobacter infection is the most frequently reported notifiable food-borne disease in humans in australia. our studies investigated the persistence of campylobacter spp. in or on darkling beetles (alphitobius diaperinus) and their larvae. our results in analyses with chickens confirm that, unless very short turnaround times are used (<72 h), beetles colonized in one production cycle (i.e., one batch of chickens) are most unlikely to still be colonized during the next cycle of chickens. | 2006 | 17012593 |
| experimental and computational assessment of conditionally essential genes in escherichia coli. | genome-wide gene essentiality data sets are becoming available for escherichia coli, but these data sets have yet to be analyzed in the context of a genome scale model. here, we present an integrative model-driven analysis of the keio e. coli mutant collection screened in this study on glycerol-supplemented minimal medium. out of 3,888 single-deletion mutants tested, 119 mutants were unable to grow on glycerol minimal medium. these conditionally essential genes were then evaluated using a genome ... | 2006 | 17012394 |
| detection and identification of food-borne pathogens of the genera campylobacter, arcobacter and helicobacter by multiplex pcr in poultry and poultry products. | the objective of this study was to develop a multiplex polymerase chain reaction (pcr) to detect and differentiate food-borne pathogens of the three genera campylobacter, arcobacter and helicobacter in a single step procedure. one common reverse primer and three genus-specific forward primers were designed by hybridizing to the 16s rrna of selected reference strains. besides the species with significance as food-borne pathogens isolated from poultry meat--campylobacter jejuni, campylobacter coli ... | 2006 | 17010041 |