Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| antiviral effect of pyran against systemic infection of mice with herpes simplex virus type 2. | the immunomodulator pyran markedly protected 5-week-old mice from lethal intravenous infection with herpes simplex virus type 2. the 50% lethal dose was increased almost 100-fold in pyran-treated mice as compared with controls. although the protection was not as marked in older mice (10 and 16 weeks old), there was a significant increase in mean survival time. when the pathogenesis of herpesvirus disease was monitored in control and drug-treated mice, the effect of pyran was most evident in the ... | 1976 | 185945 |
| a new method for the isolation of herpes simplex virus type 2 dna. | 1976 | 185791 | |
| type 1 and type 2 herpes simplex virus in corneal cell cultures. | the pathogenicity of herpes simplex virus (hsv) in primary cultures of epithelia, stromal, and endothelial cells of rabbit corneas was studied at various incubation temperatures (30 degrees, 36 degrees, and 40 degrees c). we tested three strains each of type 1 (hsv-1) and type 2 (hsv-2). at all three temperatures, the epithelial cells appeared to be more susceptible to both hsv-1 and hsv-2 than the stromal and endothelial cells. in general, less hsv-1 was requered than hsv-2 to infect the same t ... | 1976 | 185735 |
| oculogenital transmission of type 2 herpes simplex virus in adults. | type 2 (genital) herpes simplex virus (hsv-2) was isolated from three patients (two adults and an 11-year-old girl) with acute ocular infections. two of these patients had acute blepharoconjunctivitis and one had acute keratoconjunctivitis. genital herpes infections had preceded the eye infections in the two adults. this was not the case in the 11-year-old, but she had been in close contact with her sister who had apparently had the genital disease. the study strongly suggests transmission of hs ... | 1976 | 185732 |
| enzymatic basis for the selective inhibition of varicella-zoster virus by 5-halogenated analogues of deoxycytidine. | 5-bromodeoxycytidine (brdc) and 5-iododeoxycytidine, at a concentration of 100 mug/ml, effectively inhibit the replication of varicella-zoster (vz) virus in tissue culture. no toxicity could be demonstrated in uninfected cells under the same conditions. studies on the enzymatic basis for this selective inhibition were undertaken. infection of human embryonic lung cell monolayers with vz virus-infected cells results in the induction of thymidine (dt), deoxycytidine (dc), and brdc kinase activitie ... | 1976 | 185427 |
| herpes simplex virus gene expression in transformed cells. i. regulation of the viral thymidine kinase gene in transformed l cells by products of superinfecting virus. | in this paper we show that the expression of the herpes simplex virus type 1 (hsv-1) gene for thymidine kinase (tk) in hsv-transformed cells is subject to regulation by two viral products synthesized during productive infection of these cells with a tk- mutant of hsv-1. the cell line used in this study is a derivative of tk-deficient mouse l cells that, after exposure to uv-inactivated hsv-1, had acquired the hsv-1 gene for tk (which we term a resident viral gene) and consequently expressed the ... | 1976 | 185425 |
| consequences of herpes simplex virus type 2 and human cell interaction at supraoptimal temperatures. | the consequences of herpes simplex virus type 2 (hsv-2) and human embryonic fibroblast cell interaction at different temperatures (37, 40, and 42 degrees c) were investigated. incubation at 37 or 40 degrees c was permissive for hsv-2 inhibition of host dna synthesis, induction of virus-specific dna replication, and infectious virus production. the amount of [methyl-3h]thymidine incorporated into viral dna and the final yield of new infectious virus were significantly reduced at 40 degrees c comp ... | 1976 | 185418 |
| comparative diagnostic aspects of herpes simplex virus tumor-associated antigens. | sera from cancer patients and healthy individuals, obtained from two independent sources, were examined for their abilities to react with herpes simplex virus-associated tumor antigens, ag-4 and nva-taa (nonvirion antigen-tumor-associated antigen). both antigens were prepared by infection of hep-2 cells with herpes simplex virus type 2, and all antigen-antibody interactions were measured by the micro-complement fixation test. of sera from 16 patients with cancer of the uterine cervix, 81% (p les ... | 1976 | 185398 |
| differential sensitivity of herpes simplex virus types 1 and 2 to human interferon: antiviral effects of interferon plus 9-beta-d-arabinofuranosyladenine. | with use of a standard assay for antiviral compounds, in which the compound to be tested is added after absorption of virus, the minimal inhibitory concentration (mic) of human interferon for several strain of herpes simplex virus type 2 (hsv-2) is five to 10 times greater than it is for two strains of herpes simplex virus type 1 (hsv-1). this differential susceptibility of hsv types to interferon is found whether tests are done with a liquid overlay and microtiter plates or with agarose overlay ... | 1976 | 185303 |
| induction of murine p30 by superinfecting herpesviruses. | the interaction of endogenous type c viruses with superinfecting herpes simplex virus type 2 (hsv-2) was investigated in two murine cell lines. replication of hsv-2 was suboptimal in random-bred swiss/3t3a cells and, in initial experiments, infection with a low virus-to-cell ratio resulted in carrier cultures with enhanced murine leukemia virus (mulv) p30 expression. immunofluorescence tests with swiss/3t3a cells productively infected with hsv-2 also showed hsv-associated cytoplasmic antigens an ... | 1976 | 184296 |
| characterization of single-cell clonal lines derived from hsv-2-transformed mouse cells. | a balb/c continuous cell line, 238, was transformed following infection with uv-inactivated herpes simplex virus type 2 (hsv-2) was designated h238. several cell lines were derived from single cells of h238 by cloning in soft agar. the clonal lines express cytoplasmic antigens by immunofluorescence when reacted with rabbit hsv-2 antiserum and mouse tumor-bearer serum. the cloned cell lines induce tumors in immunocompetent mice by the subcutaneous route with a 100% incidence at an inoculum of 10( ... | 1975 | 184064 |
| pathogenesis of herpes simplex virus types 1 and 2 in mice after various routes of inoculation. | the pathogenesis of herpes simplex virus (hsv) types 1 and 2 was compared after inoculation of mice by different routes. intravaginal inoculation of hsv-1 and hsv-2 produced a local infection, with virus recovery from the vagina through 5 days. virus was recovered from the spinal cords 4 to 5 days after inoculation but not from liver, kidney, lung, spleen, or blood. intravenous or intraperitoneal inoculation of hsv-2 produced a focal necrotic hepatitis similar to that described previously (s. c. ... | 1976 | 184048 |
| dna-binding proteins induced by herpes simplex virus type 2 in hep-2 cells. | affinity chromatography on single-stranded and double-stranded dna-cellulose indicates that 12 proteins previously identified from herpes simplex virus type 2-infected cells, ranging in molecular weight from 28 x 10(3) to 186 x 10(3), bind to dna-cellulose. the dna-binding proteins found in infected cells differed in relative binding strengths for denatured dna-cellulose. the virus specificity of these dna-binding proteins was further studied by comparison with dna-binding proteins isolated from ... | 1976 | 183021 |
| persistent herpes simplex virus infections established in two burkitt lymphoma derived cell lines. | examination of p3hr-i cells (epstein-barr virus [ebv] producer) persistently infected with the mal strain of herpes simplex virus type i (hsv-i) suggested that only a few cells were actively producing a virus indistinguishable from hsv-i (mal) despite the presence of immunofluorescent hsv-i antigens associated with the majority of cells. ebv-specific immunofluorescence was not altered in hsv-i persistently infected p3hr-i cells. hsv-i persistently infected cells, labelled for 72 h with 14c-thymi ... | 1976 | 182914 |
| ecto-ganglioside-sialidase activity of herpes simplex virus-transformed hamster embryo fibroblasts. | cellular location of ganglioside-sialidase activity was determined in confluent hamster embryo fibroblasts transformed with herpes simplex virus type 2. approximately equal specific activities of ganglioside-sialidase activity were found to be associated with the crude lysosomal and crude plasma membrane fractions isolated from whole cell homogenates. whole transformed cells hydrolyzed exogenous ganglioside substrate, suggesting a partial location of the cellular sialidase on the outer surface o ... | 1976 | 182699 |
| adenine arabinoside (ara-a) blocked hsv-2 infected human skin fibroblasts as targets for the detection of serum mediated and cellular cytotoxicity in patients with cervical carcinoma. | human skin fibroblasts remained viable after infection with herpes simplex virus type 2 (hsv-2) if grown after infection in the presence of adenine arabinoside (ara-a); the cells were specifically killed by normal human lymphoid cells in the presence of hsv-2 convalescent or rabbit immune serum, and this cytotoxicity could be blocked by a crude hsv-2 antigen preparation. antibodies capable of mediating such cytotoxicity against hsv-2 induced cell surface antigens could be detected in four out of ... | 1976 | 182421 |
| cellular transformation in vitro induced by herpes simplex virus (hsv). note iii. oncogenicity of some hsv-2-transformed cell lines. | at high in vitro passage levels hsv-2-transformed hamster and rat cell lines were oncogenic for the homologous animals, inducing fusocellular or round-cell sarcomas. the cell lines obtained by in vitro cultivation of the tumors were free of infectious virus. | 1976 | 181902 |
| effect of zinc ions on synthesis of herpes simplex virus type 2-induced polypeptides. | 1976 | 181763 | |
| transformation of human embryonic fibroblasts by photodynamically inactivated herpes simplex virus, type 2 at supra-optimal temperature. | infection of human embryonic fibroblast cell monolayers with neutral red and light-inactivated herpes simplex virus, type 2 (hsv-2) at supra-optimal temperature (42 degrees c) resulted in persistence of viable cells in suspension culture at 37 degrees c which have properties in common with virus transformed cells: formation of cell aggregates, hsv-2-specific antigens and colony formation in soft methyl cellulose medium. these data are consistent with the idea that photodynamic inactivated hsv-2 ... | 1976 | 181535 |
| temperature-sensitive mutants of herpes simplex virus type 2: description of three new complementation groups and studies on the inhibition of host cell dna synthesis. | three new complementation groups of type 2 herpes simplex virus are described bringing the total number of complementation groups characterized to 13. of the three new groups, ts 11 fails to make virus dna at non-permissive temperature (38 degrees c) whereas ts 12 and ts 13 synthesize only very small amounts of virus or cellular dna at 38 degrees c. ts 11, like ts 9 (halliburton & timbury, 1973) fails to switch off host cell dna synthesis at 38 degrees c. that this is a failure to switch off cel ... | 1976 | 181532 |
| temperature-sensitive mutants of herpes simplex virus type 2: a provisional linkage map based on recombination analysis. | thirteen ts mutants of type 2 herpes simplex virus were backcrossed to a syncytial but not temperature-sensitive mutant of wild-type virus. this was an attempt to introduce a third marker, syncytial plaque morphology or syn, into at least some of the ts mutants. three ts mutants carrying the syn marker were obtained but only one, ts 9, was satisfactory for genetic experiments. three-factor crosses were carried out between ts 9 syn and the mutants which determined the order of eleven ts mutations ... | 1976 | 181531 |
| herpes simplex virus types 1 and 2: therapeutic response to antiviral drugs. | in vitro studies have demonstrated a relative resistance of herpes simplex virus type 2 to antiviral therapy when compared with herpes simplex virus type 1. the in vivo studies, however, show an excellent therapeutic response to both antiviral drugs in experimentally induced viral keratoconjunctivitis. | 1976 | 180942 |
| histocompatibility types and viral antibodies. | serum neutralizing (nt) antibodies to herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2), and histocompatibility (hl-a) types, were determined in multiple sclerosis (ms) patients and in control subjects. titers of nt antibodies to hsv-1 and to hsv-2 were increased among subjects bearing hl-a3 or hl-a7 or both, whether they suffered from ms or not. the increases were statistically significant in the ms group. the ms and control groups did not differ significantly in levels of hsv-1 or hsv-2 n ... | 1976 | 180933 |
| immunopotentiation and antiviral chemotherapy in a suckling rat model of herpesvirus encephalitis. | encephalitis was induced in 10-day-old wistar rats by intraperitoneal injection of approximately 100 50% tissue culture infective doses of herpes simplex virus type 2. treatment regimens included immunopotentiation with levamisole and combined therapy with levamisole and an antiviral agent, adenine arabinoside. rats treated with levamisole alone had significantly higher rates of survival than placebo-treated controls 14 days after injection of virus. combination therapy with levamisole and adeni ... | 1976 | 180203 |
| comparison of herpes simplex virus isolates using a quantitative selection assay for transformation. | transformation potential of herpes simplex virus types 1 and 2 (hsv-1 and hsv-2) was quantitatively compared in a system selecting for biochemical transformants. the highest frequency of transformation was obtained with hsv-2 strain 333. the transformation rates for two strains of hsv-1 were lower and distinctly different from hsv-2 and from each other. the dose of inactivating ultraviolet light received by the virus and the multiplicity of infection proved to be critical components of the proce ... | 1975 | 179966 |
| clonal derivatives of a herpes type 2 transformed hamster cell line (333-8-9): cytogenetic analysis, tumorigenicity and virus sequence detection. | the characteristics of the syrian hamster cell line (333-8-9), putatively transformed by herpes virus type 2 (hsv-2), strain 333, have been examined. the line has been cloned and the clones characterized cytogenetically and morphologically. the original line and the clones have been assayed for tumorigenicity and for the presence of herpes-virus-specific nucleic acid sequences using in situ hybridization with 125i hsv-2 dna. the line 333-8-9 is hyperdiploid with a mode of 49 chromosomes and cont ... | 1976 | 179958 |
| the detection of virus dna sequences in a herpes type 2 transformed hamster cell line (333-8-9). | herpes simplex virus type 2 dna labelled in vitro with 125i has been used as a probe to search for virus dna sequences in the 333-8-9 line of transformed primary hamster cells and in clines derived from the line. virus dna sequences were present when the cells were examined initially but these sequences were lost on subsequent handling. no virus sequences were detected in clones derived from the line. it is suggested that the presence of virus dna is an unstable characteristic of the cell line a ... | 1976 | 179957 |
| viruses and cancer of the lower genital tract. | the importance of viruses as oncogenic agents in animals is well established. recent work suggests that viruses may also be etiologically related to some human cancers. herpes simplex virus type 2 (hsv-2) and the genital wart virus are prime suspects in carcinomas involving the female lower genital tract. in particular, a close association has been found between hsv-2 infection and cervical neoplasia in cytohistopathologic and seroepidemiologic studies. preliminary results of prospective studies ... | 1976 | 179694 |
| [recurrent neonatal herpetic infection: isolation of herpes simplex virus type 2]. | 1975 | 179230 | |
| letter: another view of herpes simplex virus type 2. | 1976 | 178936 | |
| herpes simplex virus dna in transformed cells: sequence complexity in five hamster cell lines and one derived hamster tumor. | analyses of the hybridization kinetics of labeled herpes simplex virus 2 (hsv-2) dna with dna from five hamster cell lines transformed by uv light-irradiated hsv-2 revealed the following. (i) viral dna sequences were detected in all five cell lines tested. (ii) none of the cell lines contained the full complement of hsv-2 dna. (iii) the amount of viral dna present in the cells varied in different transformed cell lines and ranged from 8 to 32% of the hsv-2 dna genome in 1 to 3 copies/cell. (iv) ... | 1976 | 178927 |
| collaborative study of temperature-sensitive mutants of herpes simplex virus type 2. | eighteen complementation groups were identified by complementation tests and by phenotype from twenty temperature-sensitive mutants isolated independently in glasgow, scotland, and houston, tex. | 1976 | 178919 |
| electron microscope studies of temperature-sensitive mutants of herpes simplex virus type 2. | nine temperature-sensitive mutants of herpes simplex virus type 2 representing eight complementation groups were assigned to two classes as a consequence of the virion forms and virus-specific cellular alterations observed in thin sections of mutant-infected human embryonic lung cells grown at the nonpermissive temperature. mutants in class a, one dna- and one dna +, failed to synthesize detectable virus particles. mutants in class b, 4dna- and 3dna+, produced moderate to large numbers of empty ... | 1976 | 178905 |
| quantitative cytophotometry evaluated as a method for analyses of herpes simplex viral deoxyribonucleic acid synthesis. | feulgen deoxyribonucleic acid (f-dna) microspectrophotometry was evaluated as a potential tool for quantification of herpes simplex virus type 1 (hsv-1) and 2(hsv-2) dna synthesis in single cells. since hsv dna synthesis has been extensively studied using incorporation of radioactive precursors into viral and cellular dna, microspectrophotometric measures were correlated with biochemical data obtained using tritium-labeled thymidine ([3h]tdr). it was established that: (i) viral-induced increaae ... | 1976 | 178608 |
| cross-reacting herpes simplex virus antigens in hamster and mouse cells transformed by ultraviolet light-inactivated herpes simplex virus type 2. | murine and hamster cell lines, each transformed with a different strain of herpes simplex virus (hsv), were examined for cross-reacting antigens by in vitro and in vivo assays. a comparative study by the indirect immunofluorescence technique detected common cross-reacting viral antigens. cytoplasmic fluorescence patterns were observed in the 333-8-9 hamster line, the h238 murine line, and the h238 clonal lines; these patterns were identical to the fluorescence pattern of hsv -2-infected controls ... | 1976 | 178596 |
| effects of immunostimulants on resistance of newborn mice to herpes simplex type 2 infection. | since age-dependent diminished macrophage function has been related to the increased susceptibility of newborn mice to herpes simplex virus (hsv) infection, the effect of several agents which might activate macrophages or other cells involved in immunity was investigated. bcg, typhoid vaccine, brucella vaccine, levamisole, or staphage lysate were administered to newborn mice prior to challenge with hsv-2. of these agents, only bcg, administered ip or id 6 days prior to challenge, was found to in ... | 1976 | 177992 |
| herpesvirus proteins: dna polymerase and pyrimidine deoxynucleoside kinase activities in temperature-sensitive mutants of herpes simplex virus type 2. | eleven temperature-sensitive mutants of herpes simplex virus type 2 strain hg52 were examined for ability to induce dna polymerase activity in bhk 21/c13 cells. all mutants induced dna polymerase at a permissive temperature, (31 degrees c) and all dna-positive mutants at a non-permissive temperature (38 degrees c). three dna-negative mutants induced no dna polymerase (ts 6, ts 9) or very little dna polymerase (ts 11), at a non-permissive temperature, while ts 1, also dna negative, induced a litt ... | 1976 | 177730 |
| mutants of herpes simplex virus types 1 and 2 that are resistant to phosphonoacetic acid induce altered dna polymerase activities in infected cells. | three mutants of herpes simplex virus (hsv) have been isolated which form plaques in the presence of 100 mug/ml phosphonoacetic acid (ppa). all three mutants (3 from hsv-1 strain 17 syn+, 14 from hsv-1 strain 17 syn, and 19 from hsv-2) induce viral dna synthesis and viral dna polymerase activity, and these are much less sensitive to ppa than the wild-type virus. the results support the hypothesis that ppa interacts directly with the viral dna polymerase protein, at least part of which is virus c ... | 1976 | 177727 |
| [the morphology and some properties of tubular structures found in cells infected with herpes simplex virus type 2 (author's transl)]. | 1976 | 177343 | |
| electron microscope observations on tubular structures in cells infected with herpes simplex virus type 2. | the fine structure of filamentous structures and lattice-like structures found in herpes simplex virus type 2-infected cells was studied. by specimen tilting experiments, it was demonstrated that the lattice-like structures were the cross-sectional views of the tubular structures and the filamentous structures were the longitudinal sections. the observation in negatively stained preparations of isolated tubular structures revealed that the structure was constructed by regular array of ring shape ... | 1976 | 176968 |
| host range temperature-sensitive mutants of herpes simplex virus type 2. | two small-plaque mutants of herpes simplex virus type 2 (hsv-2) (strain 333), whose growth at 39 c was blocked in certain cell types (cell-dependent temperature sensitivity), were compared compared with parental virus in a number of biological assays. one mutant (no. 69) was found to produce a large number of morphologically normal, but noninfectious, particles; under nonpermissive conditions, these mutant particles were able to interfere with the replication of wild-type hsv-2. the other mutant ... | 1976 | 176473 |
| deoxyribonucleoside triphosphate pools in synchronized human cells infected with herpes simplex virus types 1 and 2. | deoxyribonucleoside triphosphate pools in uninfected and herpes simplex virus type 1 (hsv-1)- and hsv-2-infected kb cells were analyzed to determine whether ribonucleotide reductase functions in vivo in the presence and absence of thymidine (tdr). previously we showed that hsv-2 replication was inhibited in kb cells blocked in their capacity to synthesize dna by tdr. hsv-1 replication was not inhibited under these conditions. both hsv-1 and hsv-2 induced an altered ribonucleotide reductase resis ... | 1976 | 176471 |
| quantitation of the viral dna present in cells transformed by uv-irradiated herpes simplex virus. | two cell lines biochemically transformed by uv-irradiated herpes simplex virus (hsv) each contain virus dna. a comparison of the kinetics of reassociation of 3h-labeled hsv dna in the presence and absence of either clone 139 (hsv-1 transformed) or clone 207 (hsv-2 transformed) dna showed that the presence of transformed cell dna increased the rate of reassociation of approximately 10% of the viral genome while having no effect on the remaining 90%. the cot1/2 of this reaction was approximately 1 ... | 1976 | 176444 |
| igm antibody to a tumor-associated antigen (ag-4) induced by herpes simplex virus type 2: its use in location of the antigen in infected cells. | rate-zonal centrifugation was used to separate 7s and 19s lg's from sera of patients with cervical cancer and of controls. the lg's were assayed for their ability to recognize ag-4, a tumor-associated antigen, and herpes simplex virus 2 (hsv-2) by various serologic assays including neutralization, immunofluorescence, and complement fixation. these studies indicated that antibody to ag-4 is a macroglobulin; antibody-fixing complement with hsv-2 is a 7s lg. the results of various serologic assays ... | 1976 | 176392 |
| effect of cross-immunization on monotypic antibody responses to herpes simplex virus types 1 and 2. | new zealand white rabbits were immunized subcutaneously with partially purified uv-inactivated preparations of herpes simplex virus (hsv-1 or hsv-2) in complete freund's adjuvant. after the initial immunizations, designated groups of animals received additional amounts of either hsv-1 or hsv-2 at 35-day intervals. sera were absorbed with lysates of cells infected with heterotypic virus and the residual monotypic antibodies were detected by 51cr-release assay using hsv-infected target cells. a po ... | 1976 | 176275 |
| anal infections caused by herpes simplex virus. | herpes simplex infections of the perianal skin and anal canal are not uncommon, as evidenced by the present series of 16 cases, but have rarely been reported in the literature of the past. they are caused by the hsv-2 virus, which is also associated with genital lesions. there is abundant evidence that anogenital herpes is a venereal disease. anal herpes is most commonly transmitted by anal intercourse. when the infection is present inside the anal canal, especially in a primary attack, the pain ... | 1976 | 176017 |
| herpesvirus type 2-related antigens and their relevance to humoral and cell-mediated immunity in patients with cervical cancer. | the central theme of this communication is the interaction of herpes simplex virus type 2 with its host. in addition to the productive infection, we are confronted by latency and, as suggested by recent studies, by cancer. the possible mechanisms of latency and the role it may play as a precursor of carcinogenesis are discussed. if virus is to coexist with its host, a defined level of molecular interaction between host and viral gene products must exist. the association of ag-4 with active tumor ... | 1976 | 175945 |
| a search for herpes simplex virus type 2 markers in cervical carcinoma. | carcinoma of the uterine cervix is now clearly recognized as the second most common malignant disease of women in the united states. epidemiological studies provided the first suggestive evidence that an infectious, venereally transmitted agent was involved in this disease in either a causal or a casual fashion. later cytohistopathological, virological, and seroepidemiological studies confirmed this observation and identified the suspect agent as herpes simplex virus type 2. recently, several la ... | 1976 | 175943 |
| editorial: herpesvirus and cancer of uterine cervix. | frequent coitus with several partners or at a very young age carries the greatest risk of cancer of the uterine cervix. lowest risk is among celibate women and those who live in communities where marriage is stable and where premarital and extramarital coitus is infrequent. all evidence points to the venereal hypothesis of cervical cancer. herpes simplex virus type 2 (hsv-2) has been implicated. immunological defenses, oxygen tension, ph, and hormonal changes in the cervical tissues may also ... | 1976 | 175880 |
| human ecto- and endocervical epithelial cells as targets for herpes simplex type two infection in vitro. | an in vitro method was used to compare the herpes simplex virus type 2 (hsv2) susceptibility of human ecto- and endocervical epithelial cells as well as the induced cellular alterations. hsv2 caused a productive infection and morphological alterations both in ecto- and endocervical epithelial cells, but the cytopathogenicity, virus production and development of hsv2 antigens showed a greater sensitivity of ectocervical cells to hsv2 infection. the cytopathogenicity and production of infectious v ... | 1976 | 175640 |
| deoxycytidine kinase from rabbit kidney cells infected with herpes simplex virus type 1 or 2. | when rabbit kidney cells were infected with herpes simplex virus type 1 (strain seibert) or herpes simplex virus type 2 (strain 316d), deoxycytidine kinase (cdr kinase) activity, assayed at 38 degrees, increased 5- to 15-fold relative to controls. the cdr kinase activity induced by type 2 virus was more thermolabile than the enzyme activity induced by type 1 virus. when cdr kinase activity was assayed at various temperatures between 0.5 and 38 degrees, maximum activity for type 1 enzyme was obta ... | 1976 | 175378 |
| [seroepidemiological studies related to the association of genital herpes to cervical cancer (author's transl)]. | blood sera of 290 patients were examined for the presence of antibodies against herpes simplex virus type 1 and type 2 (hsv-1 and hsv-2): 1. lues - seronegative individuals, 2. patients with specific lues antibodies, and 3. patients with so-called carcinoma in situ and invasive carcinoma of the uterine cervix. the group of syphilis-seropositive individuals served as control for the group with cervical lesions, because in both groups certain socioepidemiologic factors (promiscuity, early sexual i ... | 1975 | 174969 |
| prospective double-blind evaluation of topical adenine arabinoside in male herpes progenitalis. | thirty-four virologically proven episodes of herpes progenitalis in 32 men were treated in a prospective double-blind study with either adenine arabinoside ointment or an identical-appearing placebo for 7 days. clinical evaluation and quantitative virological studies were done on days 1, 3, and 8. there was a highly significant correlation between clinical response and quantitative virology. there was no difference in clinical or virological response between drug and control groups. primary atta ... | 1975 | 174489 |
| herpes simplex virus type 2 and acute aseptic meningitis. clinical features of cases with isolation of herpes simplex virus from cerebrospinal fluids. | herpes simplex virus (hsv) was recovered from the cerebrospinal fluids (csf) of 10 patients with acute aseptic meningitis. hsv type 1 was isolated from the csf of a 6-month-old boy. the other 9 hsv isolates from csf were from adults in the age-group 15 to 29 years; 7 of these isolates were available for typing and were identified as hsv type 2. in a further 9 patients with acute aseptic meningitis and belonging to the same age-group a hsv infection was suggested by a significant titre rise in co ... | 1975 | 174191 |
| a study of the immuno-suppressive activity of herpes simplex virus type 2 and the tumor enhancing potential of the virus on yoshida sarcoma. | the relative delays of yoshida sarcoma (ys) tumor induction were used as indicator for the immunosuppressive potential caused by the subcutaneous infection of sprague-dawley rats with approximately 10(7) tcd50 hsv-2. the tumor formation is clearly accelerated and the number of tumors is increased as compared to sham injected rats. the impairment of immunity is at its maximum when the virus and the ys cells are applied simultaneously, whereas virus given after the tumor is of no consequence. the ... | 1975 | 174048 |
| dna polymerase in pseudorabies virus infected cells. | the dna polymerase activity in bhk 21/c13 cells infected with pseudorabies virus is inhibited by incubation with antiserum to pseudorabies but not by incubation with pre-immune serum or by antiserum to herpes simplex virus type 1 (hsv-1) or herpes simplex virus type 2 (hsv-2). it also differs from the cell enzyme and the enzymes in hsv-1 or hsv-2 infected cells in its requirement for kcl in the in vitro assay. it seems likely, therefore, that pseudorabies virus specifies its own dna polymerase. | 1976 | 173798 |
| antibody-dependent cell-mediated cytotoxicity to target cells infected with type 1 and type 2 herpes simplex virus. | the phenomenon of antibody-dependent cell-mediated cytoxicity (adcc) has been extended to include target cells acutely infected with herpes simplex type 1 virus (hsv-1) or herpes simplex type 2 virus (hsv-2) in an in vitro system that employs immune human serum and human blood mononuclear cells. the cytotoxic reaction was detectable after 1 hr of incubation and was complete between 4 and 8 hr. the amount of adcc noted was directly proportional to the logarithm(10) of the effector: target cell ra ... | 1976 | 173757 |
| genital herpes simplex virus type 1 infection--variability in modes of spread. | ever since the first report in 1967 of the association of herpes simplex virus type 2 (hsv-2) with urogenital infections, we have noted the occasional isolation of hsv-1 from such sites. our most recent results of the typing of hsv isolates from 527 individuals with urogenital infections show that 10.9% of such infections in females and 3.4% in males are caused by hsv-1. we describe here the acquisition of a primary oral hsv-1 infection in a male after orogenital contact with his wife who had a ... | 1975 | 173699 |
| dna polymerase induction by dna-negative temperature-sensitive mutants of herpes simplex virus type 2. | 1975 | 173076 | |
| solid phase radioimmunoassay for typing herpes simplex viral antibodies in human sera. | an indirect solid phase radioimmunoassay (ria) was developed for typing antibody to herpes simplex virus (hsv) types 1 and 2 (hsv-1 and hsv-2) in human sera. the test is based upon absorption of sera with uninfected, hsv-1-infected cells and testing for residual antibody. the high sensitivity of the ria method for detecting hsv antibody permits examination of sera at high dilutions, and thus relatively small volumes of virus-infected cells are required for cross-absorption of antibodies. results ... | 1975 | 172526 |
| the fate of herpes simplex virus type 2 dna during abortive infection at 42 degrees c of hyman embryonic lung cells. | 1975 | 171852 | |
| induction of cellular dna synthesis by a temperature-sensitive mutant of herpes simplex virus type 2. | 1975 | 171835 | |
| comparison of the virion proteins specified by herpes simplex virus types 1 and 2. | purified herpes simplex virus type 2 (hsv-2) virions were found to contain approximately the same number of polypeptides as hsv type 1 (hsv-1) virions. comparisons of the structural proteins specified by five independent hsv-2 isolates revealed some minor differences in their electrophoretic profiles on sodium dodecyl sulfate-acrylamide gels; certain invariant features of the electrophoretic profiles, however, allowed clear differentiation between all the hsv-2 isolates and hsv-1. | 1975 | 171455 |
| effect of adeno-associated virus on cancer expression by herpesvirus-transformed hamster cells. | infection of herpes simplex virus type-2-transformed hamster tumor cells with adeno-associated virus type 1 before inoculation into hamsters specifically delayed the appearance of palpable tumors and increased the survival time of the animals. the data indicated that a defective virus of humans can influence cancer expression by a virus-transformed cell. | 1975 | 171433 |
| in vivo characteristics of temperature-sensitive host range mutants of herpes simplex virus type 2. | the in vivo properties of four host range temperature-sensitive (ts) mutants of herpes simplex virus type 2 (hsv-2) were correlated with known in vitro characteristics. mutants and parental virus were inoculated into newborn and weanling mice and weanling hamsters by intracranial and subcutaneous routes, and into weanling rabbits by corneal scarification. in all cases the pattern of attenuation of mutants in vivo correlated with their stability in vitro at 39 degrees. the most attenuated mutant ... | 1975 | 171235 |
| the epidemiology and interrelationship of cervical dysplasia and type 2 herpesvirus in a low-income housing project. | this report examines the association between cervical dysplasia and herpes simplex virus type 2 antibodies in a low-income housing project, atlanta, georgia. we established a clinic in this project at which women could receive a breast examination and papanicolaou smear and have blood drawn to be tested for herpesvirus type 2 antibodies. prevalence of herpesvirus rose sharply as age increased. there was a significant absence of antibodies in young, nulliparous women. dysplasia showed a tendency ... | 1975 | 170825 |
| optimal cooling and warming rates in the preservation of herpes simplex virus (type 2). | fast rates of cooling and warming were optimal for survival of herpes simplex virus, type 2. under these conditions cryoprotectants were not necessary for virus preservation. | 1975 | 170319 |
| transformation of mouse cells after infection with ultraviolet irradiation-inactivated herpes simplex virus type 2. | a transformed mouse cell line (h238) was obtained following the infection of 238 mouse cells with ultraviolet (uv) irradiation-inactivated herpes simplex virus type 2 (hsv-2). the transformed cells produced tumors with a 100% incidence within 8 weeks in 6-week-old syngeneic balb/c mice at an inoculum of 1 times 10(6). indirect immunofluorescence (if) tests revealed the presence of hsv antigens in the transformed cells. antibodies to hsv-2 were found in the sera of tumor-bearing animals by neutra ... | 1975 | 170223 |
| [the present status of the theory of induction of carcinoma of the cervix in man by herpes virus (author's transl)]. | a large body of evidence has accumulated during the past decade suggesting that hsv-2 infection of the cervix may be a major precursor of cervical carcinoma. a brief review of the more significant findings to date is presented. | 1975 | 170159 |
| antibodies to herpes simplex virus in jewish women with cervical cancer and in healthy jewish women of israel. | herpes simplex virus (hsv) antibody titers were examined in sera from 39 jewish women with squamous cell carcinoma of the uterine cervix (cacx) and in sera from controls matched by age and country of origin. highly significant differences were found between the cases and controls for both hsv type 1 (hsv-1) and hsv type 2 (hsv-2). compared to findings in other demographic areas, the geometric mean titer (gmt) of hsv-1 among the cacx cases were considerably higher, whereas the gmt for hsv-2 was i ... | 1975 | 169365 |
| experimental infection with herpes simplex virus type 2 in newborn rats: effects of treatment with iododeoxyuridine and cytosine arabinoside. | sprague-dawley rats were inoculated, within 36 hr of birth, with either of two strains of herpes simplex virus type 2. the effects of treatment for five days with either 5-iododeoxyuridine or cytosine arabinoside in near toxic doses were studied. evaluation of treatment based on rate of survival, incidence of lesions, and isolation of virus from the central nervous system showed no appreciable difference between treated and untreated littermates. drug-related effects of retardation of growth and ... | 1975 | 169311 |
| letter: herpes simplex virus 2, (hsv2) as the carcinogen in cancer of the cerivx. | 1975 | 169222 | |
| viral gene functions expressed and detected by temperature-sensitive mutants of herpes simplex virus. | the expression of hsv-specific gene functions by 22 ts mutants of hsv-1 in 15 complementation groups and 8 ts mutants of hsv-2 in 7 complementation groups has been studied at the nonpermissive temperature. four cistrons of hsv-1 and three cistrons of hsv-2 with defects in viral dna and dan polymerase synthesis were identified. dna-mutants of hsv-1 revealed a greater alteration in hsv polypeptide synthesis and viral assembly than dna- mutants of hsv-2. mutants with apparent defects in structural ... | 1975 | 169028 |
| cervical carcinogenesis with herpes simplex virus, type 2. | in the past few years there have been a number of reports correlating a high frequency of herpes simplex virus type 2(hsv-2) infection with lesions of the uterine cervix. these studies have used a clinical history of herpetic infection or the demonstration of herpetic antibodies in the cancer patients. the present study was performed to evaluate any possible carcinogenic activity of the formalin-inoculated herpes simplex virus type 2 in the reproductive tract of the female mouse. this approach t ... | 1975 | 168521 |
| antibody patterns to herpesviruses in kaposi's sarcoma: serological association of european kaposi's sarcoma with cytomegalovirus. | sera from patients with kaposi's sarcoma (ks) were examined for antibody titres to cytomegalovirus (cmv), epstein-barr virus (ebv) and herpes simplex virus (hsv) types 1 and 2 by four techniques: indirect haemagglutination (iha), complement fixation (cf), virus neutralization (nt) and indirect immunofluorescence (if). the patients were classified, according to the stage of disease, as progressive and regressive. control sera were obtained from healthy adults, matched for age, sex, race, socioeco ... | 1975 | 166949 |
| oncogenic transformation of primary hamster cells by herpes simplex virus type 2 (hsv-2) and an hsv-2 temperature-sensitive mutant. | six oncogenically transformed cell lines were obtained following infection of hamster embryo fibroblasts with uv-irradiated herpes simplex virus type 2 (hsv-2) or with an hsv-2 temperature-sensitive mutant. all lines produced undifferentiated fibrosarcomas in newborn hamsters, four of the six lines produced metastatic tumors in the lung, and sera from tumor-bearing hamsters contained neutralizing antibody to hsv-2. hsv-specific cell-surface antigen (s) was detected by immunogluorescence tests in ... | 1975 | 166948 |
| polypeptide synthesized in herpes simplex virus type 2-infected hep-2 cells. | 1975 | 166500 | |
| latent infection of sensory ganglia with herpes simplex virus: efficacy of immunization. | mice were used to test the efficacy of active immunization in preventing latent infection of local sensory ganglia that follows inoculation of superficial epithelial surfaces with herpes simplex virus. substantial but not complete protection was observed in animals immunized and challenged with herpes simplex virus type 1, but no protection was noted in animals immunized and challenged with herpes simplex virus type 2. latent ganglionic infection can develop in immunized animals despite the pres ... | 1975 | 166432 |
| in vitro transformation of hamster cells by herpes simplex virus type 2 from human prostatic cancer cells. | a cell-associated herpes simplex virus type 2 found in a human prostatic carcinoma induced in vitro transformation of hamster embryo cells. the transformed cells (yw-74) have been shown to be hamster cells by karyotype analysis. their epithelial morphology and growth pattern, which are different from the parental cell, have remained stable through cell passages. the presence of herpesvirus antigens in the transformed cells was determined by specific immunofluorescence and colony inhibition tests ... | 1975 | 165889 |
| tumor antigens in hamsters with sarcomas associated with herpesvirus type 2. | tumor-associated antigens (tta) were demonstrated in preparations of hamster sarcomas associated with herpes simplex virus type 2, as well as in the sera of tumor-bearing hamsters. an immunoadsorption-in-gel method was employed to demonstrate and purify the taa and anti-taa. these results suggest the potential use of this technique for the demonstration of taa or of anti-taa in humans or animals with cancer. | 1975 | 165539 |
| antibody to herpes simplex virus type 2-induced nonstructural proteins in women with cervical cancer and in control groups. | sera obtained from 15 patients with cervical cancer, 10 patients with breast cancer, and 15 control women, individually matched with the cervical cancer patients, were examined for antibodies to early proteins synthesized in herpes simplex virus type 2 (hsv-2)-infected cells. the method used was an indirect radioimmune precipitation test followed by polyacrylamide gel electrophoretic analysis of immune precipitates. the relative reactivity to a major early nonstructural protein (vp134) was used ... | 1975 | 165303 |
| demonstration of oncogenic potential of mammalian cells transformed by dna-containing viruses following photodynamic inactivation. | the oncogenic properties of hamster embryo cells transformed by herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) and sv40 virus following photodynamic inactivation using neutral red were determined by subcutaneous inoculation into newborn syrian hamsters. cells transformed by all three viruses produced palpable tumors after different latent periods. histopathological examination showed that hsv-2 tumors were fibrosarcomas and metastases were often seen in the lungs. hsv-2 primary tumors we ... | 1975 | 165153 |
| temperature-sensitive host range mutants of herpes simplex virus type 2. | herpesviruses are capable of several types of infection of a host cell. to investigate the early events which ultimately determine the nature of the virus-host cell interaction, a system was established utilizing temperature-sensitive mutants of herpes simplex virus type 2. four mutants have been isolated which fail to induce cytopathic effects and do not replicate at 39 c in hamster embryo fibroblast cells. at least one mutant is virus dna negative. since intracellular complementation is detect ... | 1975 | 163920 |
| differential action of deoxynucleosides on mammalian cell cultures infected with herpes simplex virus types 1 and 2. | cytopathic effects induced by eight serologically defined isolates of herpes simplex virus type 2 (tested on human amnion cells) were markedly inhibited by thymidine at a concentration of 5 mm; eight serologically defined isolates of herpes simplex virus type 1, however, were not significantly inhibited. a similar effect was seen with thymidine, deoxyguanosine, and deoxycytidine at 1-mm concentrations in tests with rabbit kidney cultures. the inhibitory effect of thymidine was not blocked by the ... | 1975 | 163871 |
| herpes simplex virus microneutralization: a simplification of the test. | the herpes simplex virus (hsv) microneutralization test has been simplified; its use has been demonstrated for the identification of hsv isolates as type 1 (hsv-1) or type 2 (hsv-2) and for the measurement of antibodies to hsv-1 and hsv-2. in this test, the relation between the neutralization titer and virus input is linear, and thus test results can be expressed as corrected neutralization titers, rather than as the more complex neutralizing potency values previously proposed. by means of this ... | 1975 | 163870 |
| spleen cell-mediated cytotoxicity of hamster cells transformed by herpes simplex virus: evidence for virus-specific membrane antigen. | the nature of the host's immune response to isografts of hamster embryo fibroblasts (hef) transformed by herpes simplex virus type 1 (hsv-1) was investigated by the microcytotoxicity assay. it was found that spleen cells from tumor-bearing hamsters killed homologous tumor cells but not hef transformed by cytomegalovirus or para-(defective sv40)-adenovirus 7 (para-7). cytotoxicity was lost as the tumor increased in size. spleen cells from animals bearing isografts of hsv type 2 (hsv-2) transforme ... | 1975 | 163859 |
| stimulation of human lymphocytes by herpes simplex virus antigens. | lymphocytes from individuals with laboratory evidence of prior infection with herpes simplex virus (hsv) type 1 or type 2 demonstrated transformation (av antigens. higher stimulation indexes were obtained when lymphocytes were incubated with the homologous as compared with the heterologous antigen. higher mean lymphocyte stimulation indexes were also demonstrated in seropositive as compared with seronegative individuals. lymphocytes from children with hsv-1 stomatitis usually became responsive t ... | 1975 | 163788 |
| variation in susceptibility of different cell types to temperature-sensitive host range mutants of herpes simplex virus type 2. | 1975 | 163537 | |
| induction of human cell dna synthesis by herpes simplex virus type 2. | the effect of herpes simplex virus type 2 (hsv-2) infection on the synthesis of dna in human embryonic fibroblast cells was determined at temperatures permissive (37 c) and nonpermissive (42 c) for virus multiplication. during incubation of hsv-2 infected cultures at 42 c for 2 to 4 days or after shift-down from 42 to 37 c, incorporation of (3h)tdr into total dna was increased 2-to 30-fold as compared with mock-infected cultures. analysis of the (3h)dna suggested that host cell dna synthesis was ... | 1975 | 163370 |
| quantitative assay for transformation of 3t3 cells by herpes simplex virus type 2. | the interaction of herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) with swiss/3t3 cells was investigated. virus-induced cytopathic effects developed in the absence of production of infectious virus. hsv-2 inactivated with uv light (2, 4, 6, and 8 min) also induced cell death in the absence of virus replication. cell death was not detectable after infection by hsv-2 that had been inactivated by uv irradiation for 10, 12, and 14 min. 3t3 cells infected with uv-inactivated virus (10 and 12 m ... | 1975 | 163369 |
| potentiation of an adenovirus-associated virus by herpes simplex virus type-2-transformed cells. | the herpes simplex virus type-2 (hsv-2)-transformed cell line 333-8-9 was inoculated with adeno-virus-associated virus type 3 (aav-3) and examined by immunofluorescent (fa) techniques for evidence of aav-3 antigen synthesis. antisera prepared against the three sodium dodecyl sulfate-dissociated structural polypeptides (vp-1, vp-2, and vp-3) of aav-3 and against the whole aav-3 virion were used as probes to search for aav-3 fa-stainable antigens synthesized by these cells. the 333-8-9 cells inocu ... | 1975 | 163319 |
| antibody-dependent cell-mediated cytotoxicity to herpes simplex virus type 2 infected target cells in the course of cervical carcinoma. | the antibody-dependent cell-mediated cytotoxicity (adcc) assay was used as a sensitive instrument to study if specific antibodies acting synergistically with lymphocytes to induce target cell destruction, could be detected in sera from patients with cervical carcinoma. significant cytotoxicity to herpes simplex type 2 (hsv-2) infected target cells was observed in sera of 17 out of 23 (74%) surviving patients with cervical cancer who were followed with at least two serum samples. a rise in cytoly ... | 1978 | 101081 |
| detection of herpes simplex virus infection of female genitalia by the peroxidase-antiperoxidase method alone or in conjunction with the papanicolaou stain. | the sensitive peroxidase-antiperoxidase (pxapx) method individually and in conjunction with the papanicolaou (pap) stain was used to detect herpes simplex virus (hsv) in specimens from human female genitalia. initial studies using a model system of hsv-1 or hsv-2-infected vero cells established (i) acetone as the most effective fixative, (ii) optimal dilutions of preimmunization and anti-hsv serum for differentiation of infected from noninfected cells, (iii) optimal concentration of 3,3'-diamino ... | 1979 | 94600 |
| microfluorometric quantitation of size, deoxyribonucleic acid and viral antigenic determinants in cells productively infected with hsv-2. | the interaction of herpes simplex virus type 2 (hsv-2) with the eukaryotic human cell line (hep-2) was investigated by flow microfluorometric analysis. the three parameters that were quantitated include cell size, deoxyribonucleic acid content and the expression of virus-specific antigens. productively infected cells are always smaller than uninfected ones, and they resolve into two populations with respect to viral antigenic content. consistent with viral replication, one of these two populatio ... | 1979 | 94525 |
| iontophoretic application of adenine arabinoside monophosphate for the treatment of herpes simplex virus type 2 skin infections in hairless mice. | 1979 | 94335 | |
| herpes simplex virus type 2 and carcinoma of urinary bladder. | 1979 | 94309 | |
| detection of herpesvirus type-specific antibody by a micro solid phase radioimmunometric assay. | a micro solid phase radioimmunometric assay has been developed which measures type-specific antibody in human sera to herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2). the test is performed by coating wells of microtiter plates with purified hsv-specified glycoproteins, vp123 and vp119, derived from hsv-1- and hsv-2-infected cells, respectively. the coated wells are then incubated with diluted unadsorbed sera and subsequently with radioiodinated goat anti-human igg reagent. because these m ... | 1979 | 94044 |
| production of monoclonal antibodies against nucleocapsid proteins of herpes simplex virus types 1 and 2. | we prepared mouse hybrid cell lines which produced antibodies against herpes simplex virus type 1 and 2 nucleocapsids. cell lines 1d4 and 3e1, respectively, secreted immunoglobulin g1 herpes simplex virus type 1 and immunoglobulin g1 herpes simplex virus type 2 antibodies which immunoprecipitated proteins designated p40 and p45 from homologous nucleocapsid preparations but precipitated no proteins from heterologous preparations. in contrast, guinea pig antisera prepared against either herpes sim ... | 1979 | 91687 |
| observations of antigenic relatedness between viruses of the herpes simplex "neutroseron". | the antigenic relatedness of three viruses of the herpes simplex type 1 neutroseron - herpes simplex virus types 1 (hsv-1) and 2 (hsv-2) and bovine mammillitis virus (bmv) - has been examined by immune precipitation and virus neutralization tests. many virus-specific infected-cell polypeptides were shown to possess antigenic sites shared by both hsv-1 and hsv-2. cross-neutralization between the viruses is mediated through antibodies to at least two antigenic sites, one shared by hsv-1, hsv-2 and ... | 1978 | 87364 |
| a rapid technique for distinguishing herpes-simplex virus type 1 from type 2 by restriction-enzyme technology. | a rapid simplified technique, based on restriction-endonuclease analysis of radioactively labelled d.n.a. is described; it distinguishes herpes-simplex virus type 1 strains from herpes-simplex virus type 2 unambiguously. large numbers of isolates can be screened in 4--5 days. | 1979 | 86095 |