Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| two ancient rounds of polyploidy in rice genome. | an ancient genome duplication (ppp1) that predates divergence of the cereals has recently been recognized. we report here another potentially older large-scale duplication (ppp2) event that predates monocot-dicot divergence in the genome of rice (oryza sativa l.), as inferred from the age distribution of pairs of duplicate genes based on recent genome data for rice. our results suggest that paleopolyploidy was widespread and played an important role in the evolution of rice. | 0 | 15633242 |
| post-transcriptional gene silencing induced by short interfering rnas in cultured transgenic plant cells. | short interfering rna (sirna) is widely used for studying post-transcriptional gene silencing and holds great promise as a tool for both identifying function of novel genes and validating drug targets. two sirna fragments (sirna-a and -b), which were designed against different specific areas of coding region of the same target green fluorescent protein (gfp) gene, were used to silence gfp expression in cultured gfp transgenic cells of rice (oryza sativa l.; os), cotton (gossypium hirsutum l.; gh ... | 2004 | 15629049 |
| dexamethasone-inducible green fluorescent protein gene expression in transgenic plant cells. | genomic research has made a large number of sequences of novel genes or expressed sequence tags available. to investigate functions of these genes, a system for conditional control of gene expression would be a useful tool. inducible transgene expression that uses green fluorescent protein gene (gfp) as a reporter gene has been investigated in transgenic cell lines of cotton (cot; gossypium hirsutum l.), fraser fir [fra; abies fraseri (pursh) poir], nordmann fir (nor; abies nordmanniana lk.), an ... | 2004 | 15629039 |
| multigenic families and proteomics: extended protein characterization as a tool for paralog gene identification. | in classical proteomic studies, the searches in protein databases lead mostly to the identification of protein functions by homology due to the non-exhaustiveness of the protein databases. the quality of the identification depends on the studied organism, its complexity and its representation in the protein databases. nevertheless, this basic function identification is insufficient for certain applications namely for the development of rna-based gene-silencing strategies, commonly termed rna int ... | 2005 | 15627959 |
| imidazolinone-tolerant crops: history, current status and future. | imidazolinone herbicides, which include imazapyr, imazapic, imazethapyr, imazamox, imazamethabenz and imazaquin, control weeds by inhibiting the enzyme acetohydroxyacid synthase (ahas), also called acetolactate synthase (als). ahas is a critical enzyme for the biosynthesis of branched-chain amino acids in plants. several variant ahas genes conferring imidazolinone tolerance were discovered in plants through mutagenesis and selection, and were used to create imidazolinone-tolerant maize (zea mays ... | 2005 | 15627242 |
| [effects of water managements on soil nematode communities in a paddy field]. | this paper studied the effect of water managements on the abundance, trophic groups and community composition of soil nematodes in a paddy field in the lower reaches of liaohe plain at the depths of 0-10 cm, 10-20 cm and 20-30 cm during rice (oryza sativa l.) growth season. the total number of nematodes at 0-10 cm soil depth was lower in percolation-controlling treatments than in control during pre-tillage and yellow ripeness stage. no significant difference was found in the total number of nema ... | 2004 | 15624836 |
| cloning, characterization and prokaryotic expression of cytosolic malate dehydrogenase from oryza sativa. | cdna sequences of malate dehydrogenase (mdh) were cloned from various species, and mdh was identified to play an important role in cell energy metabolism. here, we present the isolation and characterization of its homologue (oscmdh) in oryza sativa. comparison of the results to the genome details indicated that oscmdh consisted of seven exons. sequence alignment showed that the deduced amino acid sequence of oscmdh shared a significant similarity with cmdh protein in zea mays, as well as with ot ... | 2004 | 15620222 |
| isolation, characterization and expression analysis of a leaf-specific phosphoenolpyruvate carboxylase gene in oryza sativa. | suppression subtractive hybridization was carried out to enrich gene fragments over-expressed in rice leaves by subtraction to rice roots, from which two identical cdna fragments were identified to encode putative phosphoenolpyruvate carboxylase. then the corresponding full-length cdna (osppc) is isolated by rt-pcr and sequenced, which indicates an open reading frame of 2895bp is contained. its deduced protein is encoded in 10 exons and shows high similarity to many other plant pepcs. comparing ... | 2004 | 15620214 |
| assessment of acetolactate synthase (als) tolerance to imazethapyr in red rice ecotypes (oryza spp) and imidazolinone tolerant/ resistant rice (oryza sativa) varieties. | three red rice ecotypes (oryza spp), including la 5, ms 5 and tx 4, were evaluated for acetolactate synthase resistance/tolerance to imazethapyr. the red rice ecotypes were compared with a tolerant line (cl-121), a resistant line (cl-161) and a conventional rice variety (cypress). based on enzymatic activity, the mean i(50) values were 1.5, 1.1, 1.5, 1.6, 20.8 and 590.6 microm imazethapyr, respectively, for la 5, ms 5, tx 4, cypress, cl-121 and cl-161. cl-161 was 32 times more resistant than cl- ... | 2005 | 15619733 |
| a novel cis-element that is responsive to oxidative stress regulates three antioxidant defense genes in rice. | all organisms have defense systems against oxidative stress that include multiple genes of antioxidant defense. these genes are induced by reactive oxygen species under condition of oxidative stress. in this study, we found that a 28-bp motif is conserved on the promoter regions of three antioxidant defense genes in rice (oryza sativa): cytosolic superoxide dismutase (sodcc1), cytosolic thioredoxin (trxh), and glutaredoxin (grx). we demonstrated that the 28-bp sequence acts as a cis-element resp ... | 2005 | 15618434 |
| complementation of sugary-1 phenotype in rice endosperm with the wheat isoamylase1 gene supports a direct role for isoamylase1 in amylopectin biosynthesis. | to examine the role of isoamylase1 (isa1) in amylopectin biosynthesis in plants, a genomic dna fragment from aegilops tauschii was introduced into the isa1-deficient rice (oryza sativa) sugary-1 mutant line em914, in which endosperm starch is completely replaced by phytoglycogen. a. tauschii is the d genome donor of wheat (triticum aestivum), and the introduced fragment effectively included the gene for isa1 for wheat (taisa1) that was encoded on the d genome. in taisa1-expressing rice endosperm ... | 2005 | 15618430 |
| annotations and functional analyses of the rice wrky gene superfamily reveal positive and negative regulators of abscisic acid signaling in aleurone cells. | the wrky proteins are a superfamily of regulators that control diverse developmental and physiological processes. this family was believed to be plant specific until the recent identification of wrky genes in nonphotosynthetic eukaryotes. we have undertaken a comprehensive computational analysis of the rice (oryza sativa) genomic sequences and predicted the structures of 81 oswrky genes, 48 of which are supported by full-length cdna sequences. eleven oswrky proteins contain two conserved wrky do ... | 2005 | 15618416 |
| effects of bacillus thuringiensis on non-target herbivore and natural enemy assemblages in tropical irrigated rice. | endotoxins from bacillus thuringiensis (bt) produced in transgenic pest-resistant bt crops are generally not toxic to predatory and parasitic arthropods. however, elimination of bt-susceptible prey and hosts in bt crops could reduce predator and parasitoid abundance and thereby disrupt biological control of other herbivorous pests. here we report results of a field study evaluating the effects of bt sprays on non-target terrestrial herbivore and natural enemy assemblages from three rice (oryza s ... | 2003 | 15612416 |
| fine mapping of a grain-weight quantitative trait locus in the pericentromeric region of rice chromosome 3. | as the basis for fine mapping of a grain-weight qtl, gw3.1, a set of near isogenic lines (nils), was developed from an oryza sativa, cv. jefferson x o. rufipogon (irgc105491) population based on five generations of backcrossing and seven generations of selfing. despite the use of an interspecific cross for mapping and the pericentromeric location of the qtl, we observed no suppression of recombination and have been able to narrow down the location of the gene underlying this qtl to a 93.8-kb reg ... | 0 | 15611185 |
| a set of three genes regulates photoperiodic responses of flowering in rice (oryza sativa). | differentiation in photoperiodic response of flowering has been key to the evolution and wide geographic distribution of rice, an essentially short-day plant. crosses were made such that the hybrid f1 plants flower later than the late-flowering parents to investigate the genetic basis underlying this differentiation. from initial experiments, three major genes for flowering time were identified from four naturally occurring variants under natural long-day conditions. an f2-derived trigenic mutan ... | 2004 | 15609572 |
| inparanoid: a comprehensive database of eukaryotic orthologs. | the inparanoid eukaryotic ortholog database (http://inparanoid.cgb.ki.se/) is a collection of pairwise ortholog groups between 17 whole genomes; anopheles gambiae, caenorhabditis briggsae, caenorhabditis elegans, drosophila melanogaster, danio rerio, takifugu rubripes, gallus gallus, homo sapiens, mus musculus, pan troglodytes, rattus norvegicus, oryza sativa, plasmodium falciparum, arabidopsis thaliana, escherichia coli, saccharomyces cerevisiae and schizosaccharomyces pombe. complete proteomes ... | 2005 | 15608241 |
| identification and fine mapping of a mutant gene for palealess spikelet in rice. | in grass, the evolutionary relationship between lemma and palea, and their relationship to the flower organs in dicots have been variously interpreted and wildely debated. in the present study, we carried out morphological and genetic analysis of a palealess mutant (pal) from rice (oryza sativa l.), and fine mapping the gene responsible for the mutated trait. together, our findings indicate that the palea is replaced by two leaf-like structures in the pal flowers, and this trait is controlled by ... | 2005 | 15605239 |
| a role of osga20ox1 , encoding an isoform of gibberellin 20-oxidase, for regulation of plant stature in rice. | gibberellin (ga) 20-oxidase (ga20ox) is a key enzyme that normally catalyzes the penultimate steps in ga biosynthesis. one of the ga20ox genes in rice (oryza sativa l.), osga20ox2 ( sd1 ), is well known as the "green revolution gene", and loss-of function mutation in this locus causes semi-dwarfism. another ga20ox gene, osga20ox1, has also been identified, but its contribution to plant stature has remained unclear because no suitable mutants have been available. we isolated a mutant, b142, tagge ... | 2004 | 15604710 |
| a genetic and structural analysis of the n-glycosylation capabilities. | the recent draft sequencing of the rice (oryza sativa) genome has enabled a genetic analysis of the glycosylation capabilities of an agroeconomically important group of plants, the monocotyledons. in this study, we have not only identified genes putatively encoding enzymes involved in n-glycosylation, but have examined by maldi-tof ms the structures of the n-glycans of rice and other monocotyledons (maize, wheat and dates; zea mays, triticum aestivum and phoenix dactylifera); these data show tha ... | 2004 | 15604706 |
| an informatics search for the low-molecular weight chromium-binding peptide. | background: the amino acid composition of a low molecular weight chromium binding peptide (lmwcr), isolated from bovine liver, is reportedly e:g:c:d::4:2:2:2, though its sequence has not been discovered. there is some controversy surrounding the exact biochemical forms and the action of cr(iii) in biological systems; the topic has been the subject of many experimental reports and continues to be investigated. clarification of cr-protein interactions will further understanding cr(iii) biochemistr ... | 2004 | 15603587 |
| [physiological and genetic analysis of lesion resembling disease mutants (lrd) of oryza sativa l]. | ten indica rice and eight japonica rice mutants with lesion resembling disease (lrd27-44) were obtained by gamma-ray mutagenesis of the whole genomes. these mutants exhibited diverse lesion mimic phenotypes under different growth environments, could be accordingly classified two types, sensitive and insensitive to environments. basing on difference in development of lesion mimics, they can be divided into three categories: whole life lesion mimics (wllm), vegetative initiation lesion mimics (vil ... | 2004 | 15599031 |
| the rice coleoptile phototropism1 gene encoding an ortholog of arabidopsis nph3 is required for phototropism of coleoptiles and lateral translocation of auxin. | we isolated a mutant, named coleoptile phototropism1 (cpt1), from gamma-ray-mutagenized japonica-type rice (oryza sativa). this mutant showed no coleoptile phototropism and severely reduced root phototropism after continuous stimulation. a map-based cloning strategy and transgenic complementation test were applied to demonstrate that a nph3-like gene deleted in the mutant corresponds to cpt1. phylogenetic analysis of putative cpt1 homologs of rice and related proteins indicated that cpt1 has an ... | 2005 | 15598797 |
| expression of bioactive human interferon-gamma in transgenic rice cell suspension cultures. | we investigated the possibility of producing the therapeutic recombinant cytokine, interferon-gamma (ifn-gamma), in transgenic rice cell (oryza sativa, cultivar tng67) suspension cultures. we tested expression of two vector constructs, each harboring an alphaamy3 leader peptide and a c-terminus his 6 tag fused to a human ifn-gamma cdna, one driven by a sucrose-starvation inducible promoter (rice alphaamy3 promoter) and the other by a constitutive maize ubiquitin promoter, in rice cell suspension ... | 2004 | 15587273 |
| the complete nucleotide sequence and multipartite organization of the tobacco mitochondrial genome: comparative analysis of mitochondrial genomes in higher plants. | tobacco is a valuable model system for investigating the origin of mitochondrial dna (mtdna) in amphidiploid plants and studying the genetic interaction between mitochondria and chloroplasts in the various functions of the plant cell. as a first step, we have determined the complete mtdna sequence of nicotiana tabacum. the mtdna of n. tabacum can be assumed to be a master circle (mc) of 430,597 bp. sequence comparison of a large number of clones revealed that there are four classes of boundaries ... | 2004 | 15583938 |
| [the structural, transcriptional and homology analysis of two frr genes in rice]. | two rice (oryza sativa subsp. japonica cv. nipponbare) ribosome recycling factor genes--osfrra and osfrrb had been identified and characterized in this study. the gene osfrra is located on chromosome 4 while osfrrb on chromosome 7. no other homologue is found in rice organelle genomes. both genes are unique in rice genome and constitutively expressed. the n-terminal character of their encoded protein products suggests that the proteins are transferred to mitochondrion and chloroplast respectivel ... | 2004 | 15583417 |
| [inducible expression of osebp-89 gene in rice]. | osebp-89 gene was an erebp (ethylene responsive elements binding protein) transcription factor from rice (oryza sativa). northern blot analysis revealed that the expression of osebp-89 gene can be induced by acc, 2,4-d, aba, br, ja, ga, 6-ba and salt. we found an ethylene responsive element-like ere (ethylene responsive element), named ivc box, in the promoter region (-552 to -510) of osebp-89 gene. emsa results showed the ivc box could specifically bind with nuclear proteins extracted from imma ... | 2004 | 15583414 |
| [genetic analysis of a heading-delayed mutant by t-dna insertion in rice (oryza sativa l.)]. | transposon tagging was used to isolate genes in higher plant. in this study, a delayed heading mutant caused by t-dna insertion in rice was identified. genetic analysis of the mutant showed that the three types of phenotype, normal heading, delayed heading and overly delayed heading in the segregating populations derived from the t-dna heterozygotes fit the ratio of 1:2:1. test for basta resistance showed the delayed heading plants were all resistant while the normal heading plants were suscepti ... | 2004 | 15583412 |
| comparison of qtl controlling seedling vigour under different temperature conditions using recombinant inbred lines in rice (oryza sativa). | seedling vigour is one of the major determinants for stable stand establishment in rice (oryza sativa), especially in a direct seeding cropping system. the objectives of this study were to identify superior alleles with consistent effects on seedling vigour across different temperature conditions and to investigate genotype x environmental temperature interactions for seedling vigour qtl. | 2005 | 15574482 |
| the jasmonate-free rice mutant hebiba is affected in the response of phya'/phya" pools and protochlorophyllide biosynthesis to far-red light. | phytochrome (phy) a in its two native isoforms (phya' and phya") and the active (pchlide(655)) and inactive (pchlide(633)) protochlorophyllides were investigated by low-temperature fluorescence spectroscopy in the tips of rice (oryza sativa l. japonica cv nihonmasari) coleoptiles from wild type (wt) and the jasmonate-deficient mutant hebiba. the seedlings were either grown in the dark or under pulsed (frp) or continuous (frc) far-red light (lambda(a) >/= 720 nm) of equal fluences. in the dark, t ... | 2015 | 15570396 |
| induction of cytoplasmic mannose-binding jacalin-related lectins is a common phenomenon in cereals treated with jasmonate methyl ester. | treatment of whole plants with jasmonic acid methyl ester induces lectin activity in leaves of oryza sativa, hordeum vulgare, triticum vulgare, secale cereale and zea mays. purification and characterization of the lectins revealed that they all have a very similar molecular structure and carbohydrate-binding properties. further analysis of the cdna clones encoding the lectins revealed that they all belong to the family of cytoplasmic mannose-specific jacalin-related lectins. | 0 | 15560260 |
| identification of genes responsive to brown planthopper nilaparvata lugens stal (homoptera: delphacidae) feeding in rice. | the brown planthopper (nilaparvata lugens stal) is a rice (oryza sativa l.)-specific herbivore that ingests assimilates from the phloem, which leads to stunted growth and therefore poor yield of rice. in this study, 27 planthopper-responsive genes in rice plants were isolated by suppression subtractive hybridization (ssh), and confirmed by filter cdna array hybridization and northern blot analysis. among these transcripts, 25 were induced and 2 were suppressed by n. lugens feeding. the responsiv ... | 2005 | 15558307 |
| functional analysis of methylthioribose kinase genes in plants. | through a biochemical and a genetic approach, we have identified several plant genes encoding methylthioribose (mtr) kinase, an enzyme involved in recycling of methionine through the methylthioadenosine (mta) cycle. osmtk1, an mtr kinase from rice (oryza sativa), is 48.6 kd in size and shows cooperative kinetics with a v(max) of 4.9 pmol/min and a k0.5 of 16.8 microm. mtr kinase genes are the first genes to be identified from the mta cycle in plants. insertional mutagenesis of the unique atmtk g ... | 2004 | 15557090 |
| the complete nucleotide sequence of wild rice (oryza nivara) chloroplast genome: first genome wide comparative sequence analysis of wild and cultivated rice. | we determined the complete nucleotide sequence of the chloroplast genome of wild rice, oryza nivara and compared it with the corresponding published sequence of relative cultivated rice, oryza sativa. the genome was 134,494 bp long with a large single-copy region of 80,544 bp, a small single-copy region of 12,346 bp and two inverted repeats of 20,802 bp each. the overall a+t content was 61.0%. the o. nivara chloroplast genome encoded identical functional genes to o. sativa in the same order alon ... | 2004 | 15556301 |
| [inheritance of bc1 gene in intersubspecific hybrids of rice (oryza sativa l.)]. | distorted segregation of the brittle culm-1 gene (bc1) on rice chromosome 3 was found with greatly increased or decreased frequency of bc1 bc1 genotype in inter-subspecific hybrids, although the gene normally transmitted to its offspring following the mendelian law in intra-subspecific hybrids. in a combination of kamairazu//ketan nangka/kamairazu,an increased frequency of bc1 bc1 in f1, normal segregation in f2, and increased and decreased frequency in a few f3 and f4 lines were observed. in a ... | 2004 | 15552049 |
| putative replication intermediates in endornavirus, a novel genus of plant dsrna viruses. | oryza sativa endornavirus (osv) belongs to a new genus (endornavirus) and family (endoviridae) with members containing large double-stranded rna (dsrna) replicons with plasmid-like properties. analysis of products obtained from in vitro reaction of the osv rna-dependent rna polymerase revealed a rapid increase of a population of the non-coding strand rna molecules with a head-to-tail composition. northern hybridization of total rna from osv-carrier cells with riboprobes specific for the coding s ... | 2004 | 15550778 |
| molecular analysis of the crinkly4 gene family in arabidopsis thaliana. | the maize (zea mays l.) crinkly4 (cr4) gene encodes a serine/threonine receptor-like kinase that controls an array of developmental processes in the plant and endosperm. the arabidopsis thaliana (l.) heynh. genome encodes an ortholog of cr4, acr4, and four crinkly4-related (crr) proteins: atcrr1, atcrr2, atcrr3 and atcrk1. the available genome sequence of rice (oryza sativa l.) encodes a cr4 ortholog, oscr4, and four crr proteins: oscrr1, oscrr2, oscrr3 and oscrr4, not necessarily orthologous to ... | 2005 | 15549374 |
| identification and characterization of a qtl on chromosome 2 for cytosolic glutamine synthetase content and panicle number in rice. | a quantitative trait locus (qtl) associated with the protein content of cytosolic glutamine synthetase (gs1; ec 6.3.1.2) in senescing leaves, panicle number, and panicle weight was characterized in rice (oryza sativa l.). a near-isogenic line (nil), c-22, developed by marker-assisted selection was grown under different nitrogen levels in the greenhouse and in a paddy field. chromosome 2 of c-22 had an approximately 50-cm segment substituted from the kasalath (indica) chromosome in a koshihikari ... | 2004 | 15549232 |
| high-resolution genetic mapping at the bph15 locus for brown planthopper resistance in rice (oryza sativa l.). | resistance to the brown planthopper (bph), nilaparvata lugens stal, a devastating sucking insect pest of rice, is an important breeding objective in rice improvement programs. bph15, one of the 17 major bph resistance genes so far identified in both cultivated and wild rice, has been identified in an introgression line, b5, and mapped on chromosome 4 flanked by restriction fragment length polymorphism markers c820 and s11182. in order to pave the way for positional cloning of this gene, we have ... | 2004 | 15549231 |
| epistasis underlying female sterility detected in hybrid breakdown in a japonica-indica cross of rice (oryza sativa l.). | epistasis is considered to be a primary genetic basis of hybrid breakdown. we found novel epistatic genes causing hybrid breakdown in an intraspecific cross of cultivated rice (oryza sativa l.). f2 progeny derived from a cross between a japonica variety, asominori, and an indica variety, ir24, showed segregation of high sterility for seeds, even though the reciprocal f1 hybrids showed about 60% seed fertility. backcross populations (bc3f2, bc3f3), obtained from repeated backcrossing with asomino ... | 2005 | 15549230 |
| chloroplast elongation factor ts pro-protein is an evolutionarily conserved fusion with the s1 domain-containing plastid-specific ribosomal protein-7. | the components of chloroplast translation are similar to those of prokaryotic translation but contain some additional unique features. proteomic analysis of the chlamydomonas reinhardtii chloroplast ribosome identified an s1-like protein, plastid-specific ribosomal protein-7 (psrp-7), as a stoichiometric component of the 30s subunit. here, we report that psrp-7 is part of a polyprotein that contains psrp-7 on its amino end and two translation elongation factor ts (ef-ts) domains at the carboxy e ... | 2004 | 15548736 |
| interactive effects of al, ca and other cations on root elongation of rice cultivars under low ph. | as with other crop species, al tolerance in rice (oryza sativa) is widely different among cultivars, and the mechanism for tolerance is unknown. the ca2+-displacement hypothesis, that is, al displaces ca2+ from critical sites in the root apoplast, was predicted to be the essential mechanism for causing al toxicity in rice cultivars. if displacement of ca is an essential cause of al toxicity in rice, al toxicity may show the same trend as toxicities of elements such as sr and ba that are effectiv ... | 2005 | 15546924 |
| sequence and comparative analysis of the maize nb mitochondrial genome. | the nb mitochondrial genome found in most fertile varieties of commercial maize (zea mays subsp. mays) was sequenced. the 569,630-bp genome maps as a circle containing 58 identified genes encoding 33 known proteins, 3 ribosomal rnas, and 21 trnas that recognize 14 amino acids. among the 22 group ii introns identified, 7 are trans-spliced. there are 121 open reading frames (orfs) of at least 300 bp, only 3 of which exist in the mitochondrial genome of rice (oryza sativa). in total, the identified ... | 2004 | 15542500 |
| promoter analysis of the barley pht1;1 phosphate transporter gene identifies regions controlling root expression and responsiveness to phosphate deprivation. | previous studies have shown that the promoter from the barley (hordeum vulgare) phosphate transporter gene, hvpht1;1, activates high levels of expression in rice (oryza sativa) roots and that the expression level was induced by up to 4-fold in response to phosphorus (p) deprivation. to identify promoter regions controlling gene regulation specificities, successive promoter truncations were made and attached to reporter genes. promoters of between 856 and 1,400 nucleotides activated gene expressi ... | 2004 | 15542491 |
| rice contains two disparate ent-copalyl diphosphate synthases with distinct metabolic functions. | rice (oryza sativa) produces ent-copalyl diphosphate for both gibberellin (ga) phytohormone and defensive phytoalexin biosynthesis, raising the question of how this initial biosynthetic step is carried out for these distinct metabolic processes. here, a functional genomics approach has been utilized to identify two disparate ent-copalyl diphosphate synthases from rice (oscps1ent and oscps2ent). notably, it was very recently demonstrated that only one of these (oscps1ent) normally operates in ga ... | 2004 | 15542489 |
| cell wall changes involved in the automorphic curvature of rice coleoptiles under microgravity conditions in space. | seedlings of rice (oryza sativa l. cv. koshihikari and cv. tan-ginbozu) were cultivated on board the space shuttle sts-95 mission and changes in the morphology and the cell wall properties of coleoptiles were analyzed. in space, rice coleoptiles showed a spontaneous (automorphic) curvature toward the caryopsis in the elongating region. the angle of automorphic curvature was larger in koshihikari than in a gibberellin-deficient dwarf cultivar, tan-ginbozu, and the angle gradually decreased during ... | 2004 | 15538652 |
| does exogenous glycinebetaine affect antioxidative system of rice seedlings under nacl treatment? | the effect of exogenously applied glycinebetaine (gb) on the alleviation of damaging effects of nacl treatment was studied in view of relative water content (rwc), malondialdehyde content, and the activity of some antioxidant enzymes in two rice (oryza sativa l.) cultivars differing in salt tolerance (salt-tolerant pokkali and--sensitive ir-28), comparatively. both cultivars took up exogenously applied gb through their roots and accumulated it to considerable levels. leaf rwc of both cultivars u ... | 2004 | 15535118 |
| characterization of oryza sativa telomerase reverse transcriptase and possible role of its phosphorylation in the control of telomerase activity. | telomerase reverse transcriptase (tert) has been characterized in the dicotyledon arabidopsis thaliana. a tert homolog has now been identified in the monocotyledon rice (oryza sativa l.) on the basis of its predicted homology to the a. thaliana enzyme (attert). at least five alternatively spliced transcripts of the rice tert (ostert) gene were detected. the full-length ostert protein shares structural features with terts of other species, including a calculated molecular size of 144 kda, an isoe ... | 2004 | 15527966 |
| computational identification and sequence analysis of stop codon readthrough genes in oryza sativa. | using an approach based on the readthrough candidate extraction system (rces), we extracted 111 candidates from 9620 gene sequences of rice. the results of homology search and sequence analysis demonstrated that these candidates included actual readthrough genes that would be important for further investigating the mechanism of translation termination regulated by readthrough event, and could also give some useful clues for functional genome annotation. between the candidates and non-candidates ... | 2004 | 15527943 |
| expression of escherichia coli branching enzyme in caryopses of transgenic rice results in amylopectin with an increased degree of branching. | physiochemical properties of starch are dependent on several factors including the relative abundance of amylose and amylopectin, and the degree of branching of amylopectin. utilizing agrobacterium-mediated transformation, a construct containing the coding region of branching enzyme of escherichia coli, under transcriptional control of the rice (oryza sativa l.) starch-branching enzyme promoter was introduced into rice cv. nakdong. to enhance glgb expression, the first intron of rice starch-bran ... | 2005 | 15517356 |
| a new 2-arylbenzofuran with antioxidant activity from the black colored rice (oryza sativa l.) bran. | from the black colored rice bran of oryza sativa cv. heugjinjubyeo, a new 2-arylbenzofuran, 2-(3,4-dihydroxyphenyl)-4,6-dihydroxybenzofuran-3-carboxylic acid methyl ester, oryzafuran (1), was isolated. its structure has been elucidated on the basis of spectral data. this compound showed strong antioxidative activity in a 1,1-diphenyl-2-picrylhydrazyl free radical scavenging assay. | 2004 | 15516765 |
| characterization of a xyloglucan endotransglucosylase gene that is up-regulated by gibberellin in rice. | xyloglucan endotransglucosylases/hydrolases (xths) that mediate cleavage and rejoining of the beta (1-4)-xyloglucans of the primary cell wall are considered to play an important role in the construction and restructuring of xyloglucan cross-links. a novel rice (oryza sativa) xth-related gene, osxth8, was cloned and characterized after being identified by cdna microarray analysis of gibberellin-induced changes in gene expression in rice seedlings. osxth8 was a single copy gene; its full-length cd ... | 2004 | 15516498 |
| identification and molecular characterization of novel anther-specific genes in oryza sativa l. by using cdna microarray. | the complicated genetic pathway regulates the developmental programs of male reproductive organ, anther tissues. to understand these molecular mechanisms, we performed cdna microarray analyses and in situ hybridization to monitor gene expression patterns during anther development in rice. microarray analysis of 4,304 cdna clones revealed that the hybridization signal of 396 cdna clones (271 non-redundant groups) increased more than six-fold in every stage of the anthers compared with that of lea ... | 2004 | 15514441 |
| genomic basis for cell-wall diversity in plants. a comparative approach to gene families in rice and arabidopsis. | monocotyledons and dicotyledons are distinct, not only in their body plans and developmental patterns, but also in the structural features of their cell walls. the recent completion of the rice (oryza sativa) genomic sequence and publication of the sequence data, together with the completed database of the arabidopsis thaliana genome, provide the first opportunity to compare the full complement of cell-wall-related genes from the two distinct classes of flowering plants. we made this comparison ... | 2004 | 15509833 |
| the gene floral organ number1 regulates floral meristem size in rice and encodes a leucine-rich repeat receptor kinase orthologous to arabidopsis clavata1. | the regulation of floral organ number is closely associated with floral meristem size. mutations in the gene floral organ number1 (fon1) cause enlargement of the floral meristem in oryza sativa (rice), resulting in an increase in the number of all floral organs. ectopic floral organs develop in the whorl of each organ and/or in the additional whorls that form. inner floral organs are more severely affected than outer floral organs. many carpel primordia develop indeterminately, and undifferentia ... | 2004 | 15509765 |
| photoperiod-regulated expression of the ppcol1 gene encoding a homolog of co/col proteins in the moss physcomitrella patens. | the constans (co) protein is a critical regulator of the photoperiodic control of flowering in arabidopsis thaliana and oryza sativa. we isolated a cdna ppcol1 encoding a homolog of the co/co-like (col) family proteins from a cryptogam physcomitrella patens. the predicted ppcol1 protein has n-terminal zinc finger and c-terminal cct domains, which are conserved in the angiosperm co/col proteins. structurally, ppcol1 is the most closely related to the group ia or ic proteins, which include atco an ... | 2004 | 15504355 |
| genomic paleontology provides evidence for two distinct origins of asian rice (oryza sativa l.). | the origin of rice domestication has been the subject of debate for several decades. we have compared the transpositional history of 110 ltr retrotransposons in the genomes of two rice varieties, nipponbare (japonica type) and 93-11 (indica type) whose complete sequences have recently been released. using a genomic paleontology approach, we estimate that these two genomes diverged from one another at least 200,000 years ago, i.e., at a time which is clearly older than the date of domestication o ... | 2004 | 15503144 |
| a rapid and efficient transformation protocol for the grass brachypodium distachyon. | a fast and efficient microprojectile bombardment-mediated transformation protocol is reported for the grass species brachypodium distachyon, a proposed alternative model plant to oryza sativa for functional genomics in grasses. embryogenic calli derived from immature embryos were transformed by a construct containing the uida (coding for beta-glucuronidase) and bar (coding for phosphinothricin acetyl transferase) genes, and bialaphos, a non-selective herbicide, was used as the selection agent th ... | 2005 | 15503032 |
| single nucleotide polymorphisms in randomly selected genes among japonica rice (oryza sativa l.) varieties identified by pcr-rf-sscp. | dna polymorphism of randomly selected genes in rice cultivars was analyzed by the polymerase chain reaction-restriction fragment-single strand conformation polymorphism (pcr-rf-sscp) technique. single dna fragments were amplified from genomic dna of the nipponbare cultivar by 671 primer pairs among the 1000 primer pairs tested. pcr-rf-sscp analysis using the 671 primer pairs detected polymorphism in 108 dna fragments between 17 japonica paddy-rice cultivars. an average of 36.9 dna fragments show ... | 2004 | 15500252 |
| effects of the interactions between selenium and phosphorus on the growth and selenium accumulation in rice (oryza sativa). | the solution culture, paddy soil culture and the simulation experiments in the laboratory were conducted to clarify the interactions between selenium and phosphorus, and its effects on the growth and selenium accumulation in rice. results revealed that a suitable supply of selenium could promote rice growth and excessive selenium could injure rice plant, causing lower biomass, especially in the roots. the supply of selenite could enhance the selenium contents of rice shoots and roots in solution ... | 2017 | 15499790 |
| mining est databases to resolve evolutionary events in major crop species. | using plant est collections, we obtained 1392 potential gene duplicates across 8 plant species: zea mays, oryza sativa, sorghum bicolor, hordeum vulgare, solanum tuberosum, lycopersicon esculentum, medicago truncatula, and glycine max. we estimated the synonymous and nonsynonymous distances between each gene pair and identified two to three mixtures of normal distributions corresponding to one to three rounds of genome duplication in each species. within the poaceae, we found a conserved duplica ... | 2004 | 15499401 |
| development and mapping of est-derived simple sequence repeat markers for hexaploid wheat. | expressed sequence tags (ests) are a valuable source of molecular markers. to enhance the resolution of an existing linkage map and to identify putative functional polymorphic gene loci in hexaploid wheat (triticum aestivum l.), over 260,000 ests from 5 different grass species were analyzed and 5418 ssr-containing sequences were identified. using sequence similarity analysis, 156 cross-species superclusters and 138 singletons were used to develop primer pairs, which were then tested on the genom ... | 2004 | 15499395 |
| calculation of binding length of base-specific dna dyes by comparison of sequence and flow cytometric data. application to oryza sativa and arabidopsis thaliana. | from biochemical experiments it has been found that at- and gc-specific dyes need a certain number of consecutive bases of the same type for binding one dye molecule. from known base sequences the amount of bases included in dye binding can be calculated and compared with experimental data from flow cytometry. oryza sativa and arabidopsis thaliana are the first higher plants which are nearly completely (>90%) sequenced. from the published sequences the theoretical fluorescence intensity of base- ... | 2005 | 15498596 |
| quantitative analyses of relationships between ecotoxicological effects and combined pollution. | the responses of wheat triticum aestivum, rice oryza sativa, earthworms eisenia foetida, and prawns penaeus japonicus to combined acetochlor-cu, cd-zn were studied in hydroponic and soil-culturing systems using the methods of ecotoxicology. in particular, systematically quantitative analyses were documented by field experiments. results showed that ecotoxicological effects under the combined pollution were not only related to chemical properties of pollutants but also dependent on the concentrat ... | 2004 | 15493474 |
| [genetic diversity of common wild rice (oryza rufipogon griff) by using ssr markers and phenotypic traits in guangxi province]. | in this paper, allelic variation and genetic diversity among geographies and growth habit types were studied by using 223 accessions of common wild rice primary core collection in guangxi province, with 34 ssr primers locating on 12 chromosomes of rice and 19 phenotypic traits. in the results, 24.91 alleles were detected per locus on average with a range from 7 to 48. compared to the cultivated rice, the wild rice showed more allelic variations. the ratio of heterozygote of ssr locus was 32.01% ... | 2004 | 15493143 |
| the trna and rrna genes in the oryza sativa genome. | in the recently assembled genomes of rice oryza sativa ssp. indica and japonica, we identified 564 and 519 trna genes, respectively. the modified wobble hypothesis, namely, at least 46 trna species must present in order to decode all 61 possible anticodons, is perfectly observed in both subspecies. among the 46 trna species, indica and japonica have many identical ones in sequence. there are 18 rice trna species that have identical counterparts in arabidopsis. in the indica superscaffold dataset ... | 2004 | 15493135 |
| characterization of soluble and putative membrane-bound udp-glucuronic acid decarboxylase (osuxs) isoforms in rice. | arabinoxylans in crop plants are the major sugar components of the cell walls, and udp-xylose is a key substrate in the biosynthesis of xylans. in this study, the six putative udp-d-glucuronic acid decarboxylase genes from rice (oryza sativa udp-xylose synthase; osuxs) were cloned. except for the soluble form of osuxs3 (genbank accession no. \ab079064), the remaining five osuxs enzymes contain a putative membrane-bound region. the six osuxs genes were classified into three types by phylogenetic ... | 2004 | 15488465 |
| [pyramiding of senescence-inhibition ipt gene and xa23 for resistance to bacterial blight in rice (oryza sativa l.)]. | transgenic lines (gc-1) carrying a senescence-inhibition cheimeric gene, ipt (isopentenyl transferase) gene, cbb23, a isogenic lines carrying xa23 gene for resistance to bacterial blight, and hexi15, a commercial cultivar showing high resistance to blast disease, were used as donors to pyramid ipt gene and xa23 by marker-assisted selection (mas). seventeen bc1f1 plants pyramiding xa23 gene and ipt genes were obtained from three multi-cross combinations. then, the plants carrying xa23 and ipt gen ... | 2004 | 15481540 |
| [endosperm-specific expression of the ferritin gene in transgenic rice (oryza sativa l.) results in increased iron content of milling rice]. | iron deficiency is the most widespread micronutrient deficiency world-wild, and is estimated to affect about 30% of the world population. to increase the iron content of rice in chinese, the 764 bp cdna of ferritin gene was cloned from soybean (phaseolus limensis), and constructed between the 1 353 bp rice glutelin glub-1 promoter and nos terminator in a binary vector pcamiba1301. the constructed pyf1067 vector was introduced into agrobacterium strain eha105, and used for transformation of the p ... | 2004 | 15478615 |
| potent antioxidative and antigenotoxic activity in aqueous extract of japanese rice bran--association with peroxidase activity. | to estimate the preventive potential of japanese rice bran (oryza sativa japonica) against the oxygen radical-related chronic diseases such as cardio-vascular diseases and cancer, antioxidative and antigenotoxic activities of the rice bran extracts were analyzed by using assay systems for lipid peroxidation and genotoxin-induced umu gene expression. when effects of the rice bran extracts under different extraction conditions on hydroperoxide generation from auto-oxidized linoleic acid were exami ... | 2004 | 15476307 |
| inheritance and qtl mapping of low temperature germinability in rice (oryza sativa l.). | quantitative trait loci (qtl) controlling low temperature germinability (ltg) in rice were identified using 81 recombinant inbreed lines (rils) derived from a cross between a japonica variety kinmaze and an indica variety dv85. the accurate condition of ltg evaluation was assumed at 15 degrees c for 10 d after removing the effect of dormancy and the second dormancy. by setting check, the scores of ltg in this study were evaluated. the germination rate at 15 degrees c for 10 d was scored to repre ... | 2004 | 15473322 |
| comparative sequence analysis of the region harboring the hardness locus in barley and its colinear region in rice. | the ancestral shared synteny concept has been advocated as an approach to positionally clone genes from complex genomes. however, the unified grass genome model and the study of grasses as a single syntenic genome is a topic of considerable controversy. hence, more quantitative studies of cereal colinearity at the sequence level are required. this study compared a contiguous 300-kb sequence of the barley (hordeum vulgare) genome with the colinear region in rice (oryza sativa). the barley sequenc ... | 0 | 15466237 |
| characterization of the silicon uptake system and molecular mapping of the silicon transporter gene in rice. | rice (oryza sativa l. cv oochikara) is a typical silicon-accumulating plant, but the mechanism responsible for the high silicon uptake by the roots is poorly understood. we characterized the silicon uptake system in rice roots by using a low-silicon rice mutant (lsi1) and wild-type rice. a kinetic study showed that the concentration of silicon in the root symplastic solution increased with increasing silicon concentrations in the external solution but saturated at a higher concentration in both ... | 2004 | 15448199 |
| some observations on an obscure disease of paddy; oryza sativa. | 1949 | 15395342 | |
| diterpene cyclases responsible for the biosynthesis of phytoalexins, momilactones a, b, and oryzalexins a-f in rice. | rice (oryza sativa l.) produces diterpene phytoalexins, such as momilactones, oryzalexins, and phytocassanes. using rice genome information and in vitro assay with recombinant enzymes, we identified genes (osks4 and osks10) encoding the type-a diterpene cyclases 9beta-pimara-7,15-diene synthase and ent-sandaracopimaradiene synthase which are involved in the biosynthesis of momilactones a, b and oryzalexins a-f respectively. transcript levels of these two genes increased remarkably after ultravio ... | 2004 | 15388982 |
| four rice genes encoding nadp malic enzyme exhibit distinct expression profiles. | in plants, the nadp malic enzymes (nadp-mes) are encoded by small gene families. these nadp-me gene families are relatively well described in c4 plants but not well studied in c3 plants. in this study, we investigated the nadp-me gene family in a model c3 monocot plant (rice, oryza sativa) based on its recently released genomic dna sequence. we found that the rice nadp-me family is composed of four members, one plastidic nadp-me and three cytosolic versions. although the rice nadp-me genes ident ... | 2004 | 15388961 |
| comparative toxicity of thiobencarb and deschlorothiobencarb to rice (oryza sativa). | 2004 | 15386094 | |
| ek-2612, a new cyclohexane-1,3-dione possessing selectivity between rice (oryza sativa) and barnyardgrass (echinochloa crus-galli). | a newly synthesized experimental compound, ek-2612 is one of the class of cyclohexane-1,3-diones which are commonly known to be grasskillers. a greenhouse study was conducted to evaluate the herbicidal performances of ek-2612 on several grass species in comparison with tralkoxydim, a commercialized cyclohexanedione derivative. like tralkoxydim, the compound ek-2612 showed excellent control efficacy on most grass weeds tested through foliar application rates between 250 and 63 g ai ha(-1). unlike ... | 2004 | 15382506 |
| inventory and comparative analysis of rice and arabidopsis atp-binding cassette (abc) systems. | atp-binding cassette (abc) proteins constitute a large superfamily found in all kingdoms of living organisms. the recent completion of two draft sequences of the rice (oryza sativa) genome allowed us to analyze and classify its abc proteins and to compare to those in arabidopsis thaliana. we identified a similar number of abc proteins in rice and arabidopsis (121 versus 120), despite the rice genome being more than three times the size of arabidopsis. both arabidopsis and rice have representativ ... | 2004 | 15381434 |
| spotted leaf11, a negative regulator of plant cell death and defense, encodes a u-box/armadillo repeat protein endowed with e3 ubiquitin ligase activity. | the rice (oryza sativa) spotted leaf11 (spl11) mutant was identified from an ethyl methanesulfonate-mutagenized indica cultivar ir68 population and was previously shown to display a spontaneous cell death phenotype and enhanced resistance to rice fungal and bacterial pathogens. here, we have isolated spl11 via a map-based cloning strategy. the isolation of the spl11 gene was facilitated by the identification of three additional spl11 alleles from an ir64 mutant collection. the predicted spl11 pr ... | 2004 | 15377756 |
| genetic diversity and phylogeny of japanese sake-brewing rice as revealed by aflp and nuclear and chloroplast ssr markers. | japanese rice ( oryza sativa l.) cultivars that are strictly used for the brewing of sake (japanese rice wine) represent a unique and traditional group. these cultivars are characterized by common traits such as large grain size with low protein content and a large, central white-core structure. to understand the genetic diversity and phylogenetic characteristics of sake-brewing rice, we performed amplified fragment length polymorphism and simple sequence repeat analyses, using 95 cultivars of l ... | 2004 | 15375619 |
| phosphorus deficiency-induced root elongation and its qtl in rice (oryza sativa l.). | a significant level of root elongation was induced in rice (oryza sativa) grown under phosphorus-deficient conditions. the root elongation clearly varied among a total of 62 varieties screened under two different phosphorus levels. two contrasting varieties, 'gimbozu', with a low elongating response and 'kasalath', with a high elongating response, were chosen and crossed to produce a hybrid population for qtl analyses. qtls for the phosphorus deficiency-induced root elongation were detected by t ... | 2004 | 15375618 |
| simple statistical models predict c-to-u edited sites in plant mitochondrial rna. | rna editing is the process whereby an rna sequence is modified from the sequence of the corresponding dna template. in the mitochondria of land plants, some cytidines are converted to uridines before translation. despite substantial study, the molecular biological mechanism by which c-to-u rna editing proceeds remains relatively obscure, although several experimental studies have implicated a role for cis-recognition. a highly non-random distribution of nucleotides is observed in the immediate v ... | 2004 | 15373947 |
| pistil induction by hormones from callus of oryza sativa in vitro. | this study describes the successful formation of floral organ pistil from the callus of pistil explants of oryza sativa l. for induction of floral organs, different explants--including young embryo, lemma, palea and pistil--were used for callus induction with different combinations of n(6)-benzyladenine and 2,4-dichlorophenoxyacetic acid (2,4-d). high frequencies of callus formation from pistil and young embryo explants were achieved. floral organs were induced after calli from pistils were tran ... | 2004 | 15365761 |
| qtls for branching, floret formation, and pre-flowering floret abortion of rice panicle in a temperate japonica x tropical japonica cross. | a large panicle with numerous florets is essential for improving rice ( oryza sativa l.) yield. rice panicle size is determined by such underlying morphogenetic processes as: (1) primary branch formation on the panicle axis; (2) floret formation on the primary branches (mainly determined by the secondary branch formation); and (3) pre-flowering abortion of florets in the panicle. we examined qtls for these processes to understand how they are integrated into panicle size. we developed 106 backcr ... | 2004 | 15365628 |
| production of bacterial blight resistant lines from somatic hybridization between oryza sativa l. and oryza meyeriana l. | novel bacterial blight (bb) resistance gene(s) for rice was (were) introduced into a cultivated japonica rice variety oryza sativa (cv. 8411), via somatic hybridization using the wild rice oryza meyeriana as the donor of the resistance gene(s). twenty-nine progenies of somatically hybridized plants were obtained. seven somatically hybridized plants and their parents were used for aflp (amplified fragment length polymorphism) analysis using 8 primer pairs. results confirmed that these plants were ... | 2004 | 15362190 |
| a novel two t-dna binary vector allows efficient generation of marker-free transgenic plants in three elite cultivars of rice (oryza sativa l.). | a pilot binary vector was constructed to assess the potential of the 2 t-dna system for generating selectable marker-free progeny plants in three elite rice cultivars (zhongzuo321, ariete and khao dawk mali 105) known to exhibit contrasting amenabilities to transformation. the first t-dna of the vector, delimited by agrobacterium tumefaciens borders, contains the hygromycin phosphotransferase (hpt) selectable gene and the green fluorescent protein (gfp) reporter gene while the second t-dna, deli ... | 2004 | 15359604 |
| ectopic expression of a cold-inducible transcription factor, cbf1/dreb1b, in transgenic rice (oryza sativa l.). | the gene encoding c-repeat/dehydration-responsive element binding factor 1 (cbf1/dreb1b) of arabidopsis was introduced into rice (oryza sativa l.) under the control of the maize ubiquitin promoter. its incorporation and expression in transgenic rice plants were confirmed by dna and rna gel-blot analyses. cold tolerance in the transgenics was not significantly different from that of the wild-type plants, as determined by ion leakage, chlorophyll fluorescence, and survival rates. however, the cold ... | 2004 | 15359131 |
| cloning and expression of a lipase gene from rice (oryza sativa cv. dongjin). | lipases are useful enzymes that are primarily responsible for the hydrolysis of acylglycerides during lipid processing. we have cloned a lipase gene from a rice seed coat cdna library (oryza sativa cv. dongjin). the cdna was 1,445 bp in length and encoded 361 amino acid residues (genbank accession no. ay580163). the deduced amino acid sequence had 82 and 56% identity to oryza sativa (cv. chuchung) and arabidopsis thaliana lipase genes, respectively, and there was a gxsxg consensus motif near the ... | 2004 | 15359122 |
| expression and purification of a novel rice (oryza sativa l.) mitochondrial atp synthase small subunit in escherichia coli. | to clarify the function of the rice mitochondrial atp synthase 6 kda subunit (rmtatp6), a method of producing large quantities of this protein is needed. here, we describe an escherichia coli expression system for the rapid and economic expression of rmtatp6. the rmtatp6 gene (genbank accession no. ) was cloned into the pgex-6p-3 vector to allow expression of rmtatp6 as a glutathione s-transferase (gst) fusion protein. the rmtatp6-gst fusion protein was purified by affinity chromatography using ... | 2004 | 15358351 |
| weed genomics: new tools to understand weed biology. | in spite of the large yield losses that weeds inflict on crops, we know little about the genomics of economically important weed species. comparative genomics between plant model species and weeds, map-based approaches, genomic sequencing and functional genomics can play vital roles in understanding and dissecting weedy traits of agronomically important weed species that damage crops. weed genomics research should increase our understanding of the evolution of herbicide resistance and of the bas ... | 2004 | 15358270 |
| diversification of genes encoding mei2 -like rna binding proteins in plants. | a predominantly plant-based family of genes encoding rna binding proteins is defined by the presence of a highly conserved rna binding motif first described in the mei2 gene of the fission yeast schizosaccharomyces pombe. in silico analyses reveal nine mei2 -like genes in arabidopsis thaliana and six in oryza sativa. these predicted genes group into four distinct clades, based on overall sequence similarity and subfamily-specific sequence elements. in situ analysis show that arabidopsis genes fr ... | 2004 | 15356386 |
| isolation and characterization of two cdnas encoding translation initiation factor 1a from rice(oryza sativa l.). | the eukaryotic initiation factor 1a(eif1a) is essential for transferring of the initiator met-trna to 40s ribosomal subunits to form the 40s pre-initiation complex. in present study, we describe the cloning and characterization of two eif1a genes from rice, which were designated as oryza sativa eukaryotic initiation factor 1a genes oseif1a-1, oseif1a-2, respectively. both rice elf1as shared high identities in amino acids with eif1a proteins from other eukaryotes. the mrna expression analysis rev ... | 2004 | 15354353 |
| expression patterns of a novel atchx gene family highlight potential roles in osmotic adjustment and k+ homeostasis in pollen development. | a combined bioinformatic and experimental approach is being used to uncover the functions of a novel family of cation/h(+) exchanger (chx) genes in plants using arabidopsis as a model. the predicted protein (85-95 kd) of 28 atchx genes after revision consists of an amino-terminal domain with 10 to 12 transmembrane spans (approximately 440 residues) and a hydrophilic domain of approximately 360 residues at the carboxyl end, which is proposed to have regulatory roles. the hydrophobic, but not the ... | 2004 | 15347787 |
| [a comparatively histological observation on the megagametophytic abortion of female-sterile rice fs-1 and its maternal parent fujisaka 5]. | a female-sterile rice fs-1, derived from the progeny of a backcross of f1 of fujisaka 5 (oryza sativa subsp. japonica, fj) and tjina (oryza sativa subsp. indica) with fj were used for histological study of megagametophytic abortion. fs-1 has a very low spikelet fertility (about 3%) and was regarded to have no true embryo sac. paraffin sectioning was adopted to study the megagametophytic development of fs-1 compared with its maternal parent fj. the materials were cultivated in field condition in ... | 2002 | 15346990 |
| [isolation of aba-regulated genes in oryza sativa through fluorescent differential display pcr (fdd-pcr)]. | abscisic acid (aba) was critical in plant seed development and response to environmental factors such as stress situations. to study the possible aba related signaling transduction pathways, we tried to isolate the aba-regulated genes through fluorescent differential display pcr (fdd-pcr) technology using rice seedling as materials (treated with aba for 2, 4, 8 and 12h). in the 17 fragments isolated, 14 and 3 clones were up-and down-regulated respectively. sequence analyses revealed that the enc ... | 2002 | 15346981 |
| prediction and identification of arabidopsis thaliana micrornas and their mrna targets. | a class of eukaryotic non-coding rnas termed micrornas (mirnas) interact with target mrnas by sequence complementarity to regulate their expression. the low abundance of some mirnas and their time- and tissue-specific expression patterns make experimental mirna identification difficult. we present here a computational method for genome-wide prediction of arabidopsis thaliana micrornas and their target mrnas. this method uses characteristic features of known plant mirnas as criteria to search for ... | 2004 | 15345049 |
| ultrastructural localization of atpase in mature tracheary elements of rice (oryza sativa l.) stamen. | the development of mature tracheary elements (tes) of rice (oryza sativa l. cv. nongkeng 58s-sd) stamen from big-vacuole to trinucleate pollen stage was studied with lead phosphate--deposition technique. at big-vacuole pollen stage, mature tes, both in filament and connective, were short of atpase activity. however, atpase activity appeared at binucleate pollen stage; in filament, one or two vascular bundle cell (s) next to te degenerated earlier than the rest; and the plasma membrane of degener ... | 2002 | 15344328 |
| biological functions of ent- and syn-copalyl diphosphate synthases in rice: key enzymes for the branch point of gibberellin and phytoalexin biosynthesis. | rice (oryza sativa l.) produces ent-copalyl diphosphate (ent-cdp) and syn-cdp as precursors for several classes of phytoalexins and the phytohormones, gibberellins (gas). it has recently been shown that a loss-of-function mutation of oscps1, a gene encoding a putative ent-cdp synthase, results in a severely ga-deficient dwarf phenotype in rice. to clarify the biological functions of the ent- and syn-cdp synthases involved in the biosynthesis of phytoalexins and/or gas, we isolated two cdnas, osc ... | 2004 | 15341631 |
| pectin methylesterases: sequence-structural features and phylogenetic relationships. | pectin methylesterases (pmes) are enzymes produced by bacteria, fungi and higher plants. they belong to the carbohydrate esterase family ce-8. this study deals with comparison of 127 amino acid sequences of this family containing the five characteristic sequence segments: 44_gxyxe, 113_qaval, 135_qdtl, 157_dfifg, 223_lgrpw (daucus carota numbering). six strictly conserved residues (gly44, gly154, asp157, gly161, arg225 and trp227) and six conservative ones (ile39, ser86, ser137, ile152, ile159 a ... | 2004 | 15337457 |
| characterization of dna end-binding activities in higher plants. | dna double-strand-breaks (dsb) are the most severe lesion in cells exposing to ionizing radiation and many other stress environments. repair of dna dsb is therefore critical to cellular survival. in this work, we observed the double-stranded dna end-binding (deb) like activities in rice (oryza sativa l. cv. tn5) suspension cells and hypocotyls from etiolated mung bean (vigna radiata l. tn5) seedlings. higher plant deb-like protein binds primarily to linearized double-stranded dna ends. competiti ... | 2004 | 15331090 |