Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| optimization and validation of a simple method using p22::luxab bacteriophage for rapid detection of salmonella enterica serotypes a, b, and d in poultry samples. | a simple method was developed for the fast and inexpensive detection of salmonella typhimurium using a recombinant p22::luxab phage. all the steps from phage production to detection were considered. a strain of salmonella typhimurium harboring the prophage p22::luxab was grown in batch culture to produce spontaneously the recombinant bacteriophage. batch production to stationary phase was better for propagation of the phage and led to a total population of 4.3 x 10(9) (+/-4.3 x 10(9)) pfu/ml of ... | 2008 | 18326191 |
| salmonella michigan soft tissue infection in an immunocompromised child. | a rare case of soft tissue infection due to salmonella michigan in an immunocompromised child is reported. the same organism was isolated from a tortoise kept in the home. immunocompromised patients are especially susceptible to reptile-associated salmonellosis and should be advised appropriately. | 2008 | 18326014 |
| epidemiological analysis of salmonella enterica from beef sampled in the slaughterhouse and retailers in dakar (senegal) using pulsed-field gel electrophoresis and antibiotic susceptibility testing. | seventy-eight isolates of salmonella spp. isolated from beef sampled from the official city slaughterhouse and from retailers in dakar, senegal were analyzed using serotyping, antimicrobial testing and macrorestriction profiling by pulsed-field gel electrophoresis (pfge). these analyses were done to identify clonal relationships and potential transmission routes in beef channel. xbai macrorestriction allowed defining 17 genotypes among the six main analyzed serotypes: salmonella bredeney (3 geno ... | 2008 | 18325615 |
| chronic enteric salmonella infection in mice leads to severe and persistent intestinal fibrosis. | intestinal fibrosis and stricture formation are serious complications of crohn's disease, often requiring surgical intervention. unfortunately, the mechanisms underlying intestinal fibrosis development are poorly understood, in part because of the lack of relevant animal models. here, we present a novel murine model of severe and persistent intestinal fibrosis caused by chronic bacterial-induced colitis. | 2008 | 18325390 |
| hypoxia-independent activation of hif-1 by enterobacteriaceae and their siderophores. | hypoxia inducible factor-1 (hif-1) is the key transcriptional regulator during adaptation to hypoxia. recent studies provide evidence for hif-1 activation during bacterial infections. however, molecular details of how bacteria activate hif-1 remain unclear. here, we pursued the role of bacterial siderophores in hif-1 activation during infection with enterobacteriaceae. | 2008 | 18325389 |
| ciprofloxacin-resistant salmonella enterica serotype typhimurium, china. | we characterized 44 salmonella enterica serotype typhimurium isolates from tongji hospital outpatients in wuhan, china, may 2002-october 2005. all 31 ciprofloxacin-resistant isolates were also resistant to > or = 8 other antimicrobial drugs and carried > or = 2 mutations in gyra and 1 mutation in parc. class 1 integrons were identified in 37 isolates. | 2008 | 18325271 |
| pyrosequencing analysis of the gyrb gene to differentiate bacteria responsible for diarrheal diseases. | pathogens causing acute diarrhea include a large variety of species from enterobacteriaceae and vibrionaceae. a method based on pyrosequencing was used here to differentiate bacteria commonly associated with diarrhea in china; the method is targeted to a partial amplicon of the gyrb gene, which encodes the b subunit of dna gyrase. twenty-eight specific polymorphic positions were identified from sequence alignment of a large sequence dataset and targeted using 17 sequencing primers. of 95 isolate ... | 2008 | 18324428 |
| high-throughput genotyping of salmonella enterica serovar typhi allowing geographical assignment of haplotypes and pathotypes within an urban district of jakarta, indonesia. | high-throughput epidemiological typing systems that provide phylogenetic and genotypic information are beneficial for tracking bacterial pathogens in the field. the incidence of salmonella enterica serovar typhi infection in indonesia is high and is associated with atypical phenotypic traits such as expression of the j and the z66 flagellum antigens. utilizing a high-throughput genotyping platform to investigate known nucleotide polymorphisms dispersed around the genome, we determined the haplot ... | 2008 | 18322069 |
| characterization of salmonella enterica serovar agona slaughter isolates from the animal arm of the national antimicrobial resistance monitoring system-enteric bacteria (narms): 1997 through 2003. | a total of 499 salmonella enterica serovar agona isolates from cattle, swine, chicken, and turkey samples were assayed for antimicrobial susceptibility and subtyped using pulsed-field gel electrophoresis (pfge). salmonella agona isolates exhibited increased resistance to amoxicillin/clavulanic acid, ampicillin, cefoxitin, ceftiofur, cephalothin, and chloramphenicol, and a single isolate was resistant to ceftriaxone. multiple drug resistance (mdr; resistance >or= 2 antimicrobials) was exhibited i ... | 2008 | 18321206 |
| efficient immobilization and patterning of live bacterial cells. | a monolayer of live bacterial cells has been patterned onto substrates through the interaction between cfa/i fimbriae and the corresponding antibody. patterns of live bacteria have been prepared with cellular resolution on silicon and gold substrates for salmonella enterica serovar typhimurium as a model with high specificity and efficiency. the immobilized cells are capable of dividing in growth medium to form a self-sustaining bacterial monolayer on the patterned areas. interestingly, the immo ... | 2008 | 18321142 |
| heterologous prime-boost immunizations with different salmonella serovars for enhanced antigen-specific cd8 t-cell induction. | pre-existing anti-vector immunity can severely compromise the ability of salmonella enterica serovar typhimurium live vaccines to induce protective cd8 t-cell frequencies after type iii secretion system-mediated heterologous protein translocation in orally immunized mice. in the present study, we demonstrate that heterologous prime-boost immunizations using attenuated serovar typhimurium and serovar dublin strains for foreign antigen delivery can be employed to bypass anti-salmonella immunity re ... | 2008 | 18313813 |
| salmonella enterica serovar choleraesuis derivatives harbouring deletions in rpos and phop regulatory genes are attenuated in pigs, and survive and multiply in porcine intestinal macrophages and fibroblasts, respectively. | live attenuated salmonella enterica strains have been extensively studied as potential vectors for the oral delivery of heterologous antigens. due to its ability to target immune cells, its specific mechanism for crossing the intestinal barrier, and its swine-restricted tropism, s. enterica subspecies enterica serovar choleraesuis (s. choleraesuis) has attracted a great deal of interest for the production of bacterial-based oral carriers specifically adapted to swine. in this study, two mutants ... | 2008 | 18313237 |
| role of outer membrane lipopolysaccharides in the protection of salmonella enterica serovar typhimurium from desiccation damage. | the ability to survive desiccation between hosts is often essential to the success of pathogenic bacteria. the bacterial outer membrane is both the cellular interface with hostile environments and the focus of much of the drying-induced damage. this study examined the contribution of outer membrane-associated polysaccharides to the survival of salmonella enterica serovar typhimurium in air-dried blood droplets following growth in high and low osmolarity medium and under conditions known to induc ... | 2008 | 18312578 |
| the prpz gene cluster encoding eukaryotic-type ser/thr protein kinases and phosphatases is repressed by oxidative stress and involved in salmonella enterica serovar typhi survival in human macrophages. | the prpz gene cluster consists of three orfs coding for proteins with homology to eukaryotic-type ser/thr protein phosphatases 2c (prpz) and ser/thr protein kinases (prky and prkx). this cluster is present in the sequenced genomes of salmonella enterica serovar typhi (s. typhi) strains ty2 and ct18. this study investigated the genetic organization of this gene cluster, its regulation and its putative involvement in virulence. the three genes are transcribed as a polycistronic mrna as demonstrate ... | 2008 | 18312574 |
| leaf age as a risk factor in contamination of lettuce with escherichia coli o157:h7 and salmonella enterica. | outbreaks of escherichia coli o157:h7 infections have been linked increasingly to leafy greens, particularly to lettuce. we present here the first evidence that this enteric pathogen can multiply on the leaves of romaine lettuce plants. the increases in population size of e. coli o157:h7 in the phyllosphere of young lettuce plants ranged from 16- to 100-fold under conditions of warm temperature and the presence of free water on the leaves and varied significantly with leaf age. the population si ... | 2008 | 18310433 |
| interactions between food-borne pathogens and protozoa isolated from lettuce and spinach. | the survival of salmonella enterica was recently shown to increase when the bacteria were sequestered in expelled food vacuoles (vesicles) of tetrahymena. because fresh produce is increasingly linked to outbreaks of enteric illness, the present investigation aimed to determine the prevalence of protozoa on spinach and lettuce and to examine their interactions with s. enterica, escherichia coli o157:h7, and listeria monocytogenes. glaucoma sp., colpoda steinii, and acanthamoeba palestinensis were ... | 2008 | 18310421 |
| aro mutations in salmonella enterica cause defects in cell wall and outer membrane integrity. | in this study we characterized aro mutants of salmonella enterica serovars enteritidis and typhimurium, which are frequently used as live oral vaccines. we found that the aroa, arod, and aroc mutants were sensitive to blood serum, albumen, edta, and ovotransferrin, and this defect could be complemented by an appropriate aro gene cloned in a plasmid. subsequent microarray analysis of gene expression in the arod mutant in serovar typhimurium indicated that the reason for this sensitivity might be ... | 2008 | 18310348 |
| identification of novel genes and pathways affecting salmonella type iii secretion system 1 using a contact-dependent hemolysis assay. | we screened 5,700 salmonella enterica serovar typhimurium mutants for defects in type iii secretion system 1 (t3ss-1)-mediated contact-dependent hemolysis to identify novel genes and pathways affecting the activity of t3ss-1. our data suggest that previously unrecognized factors such as type i fimbriae may modulate the expression, activity, or deployment of this key virulence factor. | 2008 | 18310344 |
| characterization of the periplasmic domain of motb and implications for its role in the stator assembly of the bacterial flagellar motor. | mota and motb are integral membrane proteins that form the stator complex of the proton-driven bacterial flagellar motor. the stator complex functions as a proton channel and couples proton flow with torque generation. the stator must be anchored to an appropriate place on the motor, and this is believed to occur through a putative peptidoglycan-binding (pgb) motif within the c-terminal periplasmic domain of motb. in this study, we constructed and characterized an n-terminally truncated variant ... | 2008 | 18310339 |
| small outer-membrane lipoprotein, smpa, is regulated by sigmae and has a role in cell envelope integrity and virulence of salmonella enterica serovar typhimurium. | smpa is a small outer-membrane lipoprotein that is a component of the essential yaet outer-membrane protein assembly complex. in salmonella enterica serovar typhimurium (s. typhimurium), expression of the smpa gene was shown to be directed by two promoters, smpap1 and smpap2. the more distal promoter, smpap1, is dependent upon the extracytoplasmic stress response sigma factor sigma(e). an smpa null mutant was constructed in s. typhimurium sl1344 and was shown to be more sensitive than its wild-t ... | 2008 | 18310044 |
| the pdux enzyme of salmonella enterica is an l-threonine kinase used for coenzyme b12 synthesis. | here, the pdux enzyme of salmonella enterica is shown to be an l-threonine kinase used for the de novo synthesis of coenzyme b(12) and the assimilation of cobyric acid (cby). pdux with a c-terminal his tag (pdux-his(6)) was produced at high levels in escherichia coli, purified by nickel affinity chromatography, and partially characterized. (31)p nmr spectroscopy established that purified pdux-his(6) catalyzed the conversion of l-threonine and atp to l-threonine-o-3-phosphate and adp. enzyme assa ... | 2008 | 18308727 |
| the salmonella-containing vacuole: moving with the times. | salmonella pathogenesis is dependent on its ability to invade and replicate within host cells. following invasion the bacteria remain within a modified phagosome known as the salmonella-containing vacuole (scv), within which they will survive and replicate. invasion and scv biogenesis are dependent on two type iii secretion systems, t3ss1 and t3ss2, which are used to translocate distinct cohorts of bacterial effector proteins into the host cell. elucidating the roles of individual effector prote ... | 2008 | 18304858 |
| role of soil, crop debris, and a plant pathogen in salmonella enterica contamination of tomato plants. | in the u.s., tomatoes have become the most implicated vehicle for produce-associated salmonellosis with 12 outbreaks since 1998. although unconfirmed, trace backs suggest pre-harvest contamination with salmonella enterica. routes of tomato crop contamination by s. enterica in the absence of direct artificial inoculation have not been investigated. | 2008 | 18301739 |
| detection of sublethal thermal injury in salmonella enterica serotype typhimurium and listeria monocytogenes using fourier transform infrared (ft-ir) spectroscopy (4000 to 600 cm(-1)). | fourier transform infrared (ft-ir) spectroscopy (4000 to 600 cm(-1)) was utilized to detect sublethally heat-injured microorganisms: salmonella enterica serotype typhimurium atcc 14028, a gram-negative bacterium, and listeria monocytogenes atcc 19113, a gram-positive bacterium. a range of heat treatments (n= 2) at 60 degrees c were evaluated: 0d (control), 2d, 4d, 6d, and 8d using a d(60 degrees c) (s. enterica serotype typhimurium atcc 14028 = 0.30 min, l. monocytogenes atcc 19113 = 0.43 min). ... | 2008 | 18298736 |
| inactivation of salmonella enterica ser. enteritidis in tomato juice by combining of high-intensity pulsed electric fields with natural antimicrobials. | the effect of high-intensity pulsed electric field (hipef) treatment (35kv/cm, 4 mus pulse length in bipolar mode without exceeding 38 degrees c) as influenced by treatment time (200, 600, and 1000 micros) and pulse frequency (100, 150, and 200 hz) for inactivating salmonella enterica ser. enteritidis inoculated in tomato juice was evaluated. similarly, the effect of combining hipef treatment with citric acid (0.5%, 1.0%, 1.5%, and 2.0%[wt/vol]) or cinnamon bark oil (0.05%, 0.10%, 0.2%, and 0.3% ... | 2008 | 18298735 |
| caspase-3-dependent phagocyte death during systemic salmonella enterica serovar typhimurium infection of mice. | growth of salmonella enterica in mammalian tissues results from continuous spread of bacteria to new host cells. our previous work indicated that infective s. enterica are liberated from host cells via stochastic necrotic burst independently of intracellular bacterial numbers. here we report that liver phagocytes can undergo apoptotic caspase-3-mediated cell death in vivo, with apoptosis being a rare event, more prevalent in heavily infected cells. the density-dependent apoptotic cell death is l ... | 2008 | 18298550 |
| display of heterologous proteins on the surface of lactococcus lactis using the h and w domain of prtb from lactobacillus delburueckii subsp. bulgaricus as an anchoring matrix. | the aim of this study was to develop a cell-surface display system for foreign antigens on the surface of a lactococcus lactis strain using an h and w domain of prtb from lactobacillus delburueckii subsp. bulgaricus as an anchoring matrix. | 2008 | 18298534 |
| viability of zoonotic pathogens escherichia coli and salmonella in swine manure slurries with and without a urease inhibitor and thymol. | to determine the effects of urine, a urease inhibitor and/or an odour-reducing antimicrobial compound, on zoonotic pathogens in swine manure slurries. | 2008 | 18298450 |
| microcompartments for b12-dependent 1,2-propanediol degradation provide protection from dna and cellular damage by a reactive metabolic intermediate. | salmonella enterica grows on 1,2-propanediol (1,2-pd) in a coenzyme b(12)-dependent fashion. prior studies showed that a bacterial microcompartment (mcp) is involved in this process and that an mcp-minus mutant undergoes a 20-h period of growth arrest during 1,2-pd degradation. it was previously proposed that growth arrest resulted from propionaldehyde toxicity, but no direct evidence was presented. here, high-pressure liquid chromatography analyses of culture medium were used to show that the m ... | 2008 | 18296526 |
| yjgf is required for isoleucine biosynthesis when salmonella enterica is grown on pyruvate medium. | the yjgf/yer057c/uk114 family of proteins is conserved across the three domains of life, yet no biochemical function has been clearly defined for any member of this family. in salmonella enterica, a deletion of yjgf results in a requirement for isoleucine when the mutant strain is grown in glucose-serine or pyruvate medium. feedback inhibition of ilva is required for the curative effect of isoleucine on glucose-serine medium. on pyruvate medium, yjgf mutants are unable to synthesize enough isole ... | 2008 | 18296521 |
| construction and use of new cloning vectors for the rapid isolation of recombinant proteins from escherichia coli. | we describe the construction and use of two sets of vectors for the over-expression and purification of protein from escherichia coli. the set of ptev plasmids (ptev3, 4, 5) directs the synthesis of a recombinant protein with a n-terminal hexahistidine (his(6)) tag that is removable by the tobacco etch virus (tev) protease. the set of pkld plasmids (pkld66, 116) directs the synthesis of a recombinant protein that contains a n-terminal his(6) and maltose-binding protein tag in tandem, which can a ... | 2008 | 18295882 |
| terpenoid compositions, and antioxidant and antimicrobial properties of the rhizome essential oils of different hedychium species. | a phytochemical study of the rhizome essential oils of four different hedychium species was performed by means of gc and gc/ms analyses. h. ellipticum mainly contained 1,8-cineole, sabinene, and terpin-4-ol, while h. aurantiacum possessed terpin-4-ol, para-cymene, and bornyl acetate as the major entities. similarly, trans-meta-mentha-2,8-diene and linalool were noticed in h. coronarium. three different collections (i-iii) of h. spicatum showed amazing differences in the relative contents of thei ... | 2008 | 18293443 |
| infection of epithelial cells with salmonella enterica. | salmonella enterica serovars cause a variety of diseases ranging from self-limiting gastroenteritis to severe systemic infections. virulence of these facultative intracellular pathogens is dependent on their ability to invade and replicate within non-phagocytic cells, and cultured epithelial cell systems have been used extensively to dissect the molecular mechanisms involved. for efficient invasion in vitro, the bacterial cell growth conditions as critical since the invasion associated type iii ... | 2008 | 18287758 |
| nitric oxide homeostasis in salmonella typhimurium: roles of respiratory nitrate reductase and flavohemoglobin. | nitric oxide (no) is generated in biological systems primarily via the activity of no synthases and nitrate and nitrite reductases. here we show that salmonella enterica serovar typhimurium (s. typhimurium) grown anaerobically with nitrate is capable of generating polarographically detectable no after nitrite (no(2)(-)) addition. no accumulation is sensitive to the no scavenger 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide. neither an fnr mutant nor an fnr hmp double mutant p ... | 2008 | 18285340 |
| flil is essential for swarming: motor rotation in absence of flil fractures the flagellar rod in swarmer cells of salmonella enterica. | flil is the first gene in a flagellar operon that specifies members of the switch complex and type iii export system in salmonella enterica and escherichia coli, but no function has been ascribed to this gene thus far. here we report that a flil mutant is slightly impaired for swimming but completely defective in swarming in both organisms, and have studied this phenotype further in s. enterica. we have found that on swarm agar, mutant cells release or 'eject' their flagellar filaments. the rele ... | 2008 | 18284590 |
| spvc is a salmonella effector with phosphothreonine lyase activity on host mitogen-activated protein kinases. | spvc is encoded by the salmonella virulence plasmid. we have investigated the biochemical function of spvc and the mechanism by which it is secreted by bacteria and translocated into infected macrophages. we constructed a strain carrying a deletion in spvc and showed that the strain is attenuated for systemic virulence in mice. spvc can be secreted in vitro by either the spi-1 or spi-2 type iii secretion systems. cell biological and genetic experiments showed that translocation of the protein in ... | 2008 | 18284579 |
| growth control in the salmonella-containing vacuole. | salmonella enterica is an intracellular bacterial pathogen that inhabits membrane-bound vacuoles of eukaryotic cells. coined as the 'salmonella-containing vacuole' (scv), this compartment has been studied for two decades as a replicative niche. recent findings reveal, however, marked differences in the lifestyle of bacteria enclosed in the scv of varied host cell types. in fibroblasts, the emerging view supports a model of bacteria facing in the scv a 'to grow' or 'not to grow' dilemma, which is ... | 2008 | 18282735 |
| nucleotide biosynthesis is critical for growth of bacteria in human blood. | proliferation of bacterial pathogens in blood represents one of the most dangerous stages of infection. growth in blood serum depends on the ability of a pathogen to adjust metabolism to match the availability of nutrients. although certain nutrients are scarce in blood and need to be de novo synthesized by proliferating bacteria, it is unclear which metabolic pathways are critical for bacterial growth in blood. in this study, we identified metabolic functions that are essential specifically for ... | 2008 | 18282099 |
| salmonella species in free-living spur-thighed tortoises (testudo graeca) in central western morocco. | 2008 | 18281632 | |
| influence of housing system, grain type, and particle size on salmonella colonization and shedding of broilers fed triticale or corn-soybean meal diets. | salmonella colonization in poultry may be influenced by grain type and particle size. broilers reared either in nonlitter cage-based housing or in a conventionally floored litter house from 0 to 42 d were assigned to 1 of 4 dietary treatments: 1) ground corn-soybean meal (c, 560 microm), 2) coarsely ground corn-soybean meal (cc, >1,700 microm), 3) ground triticale-soybean meal (t, 560 microm), or 4) whole triticale-soybean meal (wt). a 4-strain cocktail of salmonella enterica was orally gavaged ... | 2008 | 18281566 |
| emergence of highly fluoroquinolone-resistant salmonella enterica serovar typhi in a community-based fever surveillance from kolkata, india. | 2008 | 18280709 | |
| parallel evolution of multidrug-resistance in salmonella enterica isolated from swine. | the increase in frequency of salmonella enterica resistant to antibiotics in food-producing animals is of great concern to public health. determining the rate at which different resistance phenotypes are generated and maintained in the environment is thus of great importance. the distribution and evolution of antibiotic resistance and multidrug-resistance in 362 salmonella stains as part of a cross-sectional study of the canadian swine industry were investigated. the susceptibility of all isolat ... | 2008 | 18279339 |
| simple, rapid, and affordable point-of-care test for the serodiagnosis of typhoid fever. | we developed a point-of-care test for the serodiagnosis of typhoid fever in the format of an immunochromatographic lateral flow assay. the flow assay for typhoid fever is based on the detection of salmonella enterica serotype typhi lipopolysaccharide-specific immunoglobulin m (igm) antibodies. the assay was evaluated on serum samples collected in a hospital in south sulawesi, indonesia, where typhoid fever is endemic, and the results were compared with culture and widal test. the sensitivity of ... | 2008 | 18276100 |
| salmonella enterica serovar senftenberg human clinical isolates lacking spi-1. | nontyphoidal salmonella species cause gastrointestinal disease worldwide. the prevailing theory of salmonella enteropathogenesis is that bacterial invasion of the intestinal epithelium is essential for virulence and that this requires the virulence-associated genomic region salmonella pathogenicity island 1 (spi-1). recent studies of salmonella enterica infection models have demonstrated that enterocolitis and diarrhea in mice and cows can occur independently of spi-1. in this study, we sought t ... | 2008 | 18272702 |
| tolc, but not acrb, is involved in the invasiveness of multidrug-resistant salmonella enterica serovar typhimurium by increasing type iii secretion system-1 expression. | the acrab-tolc efflux system is involved in multidrug and bile salt resistances. in addition, this pump has recently been suggested to increase the invasion of salmonella enterica serovar typhimurium (s. typhimurium) into host cells in vitro and could therefore have an important clinical relevance for multidrug-resistant strains. the aim of this study was to investigate the role of the tolc outer membrane channel and the acrb transporter in the interaction of multidrug-resistant s. typhimurium s ... | 2008 | 18272427 |
| overcoming h-ns-mediated transcriptional silencing of horizontally acquired genes by the phop and slya proteins in salmonella enterica. | the acquisition of new traits through horizontal gene transfer depends on the ability of the recipient organism to express the incorporated genes. however, foreign dna appears to be silenced by the histone-like nucleoid-structuring protein (h-ns) in several enteric pathogens, raising the question of how this silencing is overcome and the acquired genes are expressed at the right time and place. to address this question, we investigated transcription of the horizontally acquired ugtl and pagc gen ... | 2008 | 18270203 |
| subcutaneous vaccination with attenuated salmonella enterica serovar choleraesuis c500 expressing recombinant filamentous hemagglutinin and pertactin antigens protects mice against fatal infections with both s. enterica serovar choleraesuis and bordetella bronchiseptica. | salmonella enterica serovar choleraesuis strain c500 is a live, attenuated vaccine that has been used in china for over 40 years to prevent piglet paratyphoid. we compared the protective efficacies of subcutaneous (s.c.) and oral vaccination of balb/c mice with c500 expressing the recombinant filamentous hemagglutinin type i domain and pertactin region 2 domain antigen (rf1p2) of bordetella bronchiseptica. protective efficacy against both s. enterica serovar choleraesuis infection in an oral fat ... | 2008 | 18268026 |
| the capacity of salmonella to survive inside dendritic cells and prevent antigen presentation to t cells is host specific. | infection with salmonella enterica serovar typhimurium (s. typhimurium) causes a severe and lethal systemic disease in mice, characterized by poor activation of the adaptive immune response against salmonella-derived antigens. recently, we and others have reported that this feature relies on the ability of s. typhimurium to survive within murine dendritic cells (dcs) and avoid the presentation of bacteria-derived antigens to t cells. in contrast, here we show that infection of murine dcs with ei ... | 2008 | 18266715 |
| architectural adaptation and protein expression patterns of salmonella enterica serovar enteritidis biofilms under laminar flow conditions. | salmonella enterica serovar enteritidis is a significant biofilm-forming pathogen. the influence of a 10-fold difference in nutrient laminar flow velocity on the dynamics of salmonella enteritidis biofilm formation and protein expression profiles were compared in order to ascertain how flow velocity influenced biofilm structure and function. low-flow (0.007 cm s(-1)) biofilms consisted of diffusely-arranged microcolonies which grew until merging by approximately 72 h. high-flow (0.07 cm s(-1)) b ... | 2008 | 18261816 |
| evaluation of the premi test salmonella, a commercial low-density dna microarray system intended for routine identification and typing of salmonella enterica. | a new commercial system based on genetic profiling and aimed at identifying salmonella enterica serovars was evaluated by comparing its performance with classical serotyping on 443 strains. within 62 serovars represented, 60 gave unique genetic profiles while 2 were undistinguishable. results were obtained within 8 h, were reproducible and clear-cut. the system allowed single-tube processing of the samples and required no peculiar technical skill. it showed interesting potential for routine labo ... | 2008 | 18258323 |
| plasmid-mediated quinolone resistance in salmonella enterica, united kingdom. | 2008 | 18258138 | |
| enhanced subtyping scheme for salmonella enteritidis. | to improve pulsed-field gel electrophoresis-based strain discrimination of 76 salmonella enteritidis strains, we evaluated 6 macro-restriction endonucleases, separately and in various combinations. one 3-enzyme subset, sfii/paci/noti, was highly discriminatory. five different indices, including the simpson diversity index, supported this 3-enzyme combination for improved differentiation of s. enteritidis. | 2007 | 18258051 |
| multidrug-resistant salmonella typhimurium, pacific northwest, united states. | we compared human and bovine isolates of salmonella enterica using antimicrobial-drug resistance profiles and pulsed-field gel electrophoresis. from 2000 through 2006, we observed an increase in a novel multidrug-resistant clone of s. typhimurium with no recognized phage type. this clone may represent an emerging epidemic strain in the pacific northwest. | 2007 | 18258014 |
| impact of dietary components on chicken immune system and salmonella infection. | salmonella enterica serovars are facultative intracellular pathogens that may cause serious illness in poultry and humans. human infection by two common serovars, salmonella enteritidis (se) and salmonella typhimurium (st) usually occurs via food-borne transmission. consumption of raw or undercooked contaminated eggs usually causes se infection, while st is transmitted by contaminated chicken meat. there are several reports on dietary interventions, including fatty acid modifications, probiotic ... | 2008 | 18251669 |
| molecular characterization reveals salmonella enterica serovar 4,[5],12:i:- from poultry is a variant typhimurium serovar. | although salmonella remains one of the leading causes of foodborne illnesses in the united states, the salmonella enterica serovars and genetic types associated with most infections appear to fluctuate over time. recently, the center for disease control and prevention (cdc) has reported an increase in cases of salmonellosis caused by salmonella 4,[5],12:i:-. similarly, this unusual salmonella serovar has been isolated from cattle and poultry in the state of georgia. we examined the genetic relat ... | 2007 | 18251408 |
| secreted enteric antimicrobial activity localises to the mucus surface layer. | the intestinal mucosa is constantly exposed to a dense and highly dynamic microbial flora and challenged by a variety of enteropathogenic bacteria. antibacterial protection is provided in part by paneth cell-derived antibacterial peptides such as the alpha-defensins. the mechanism of peptide-mediated antibacterial control and its functional importance for gut homeostasis has recently been appreciated in patients with crohn's ileitis. in the present study, the spatial distribution of antimicrobia ... | 2008 | 18250125 |
| identification of salmonella spi-2 secretion system components required for spvb-mediated cytotoxicity in macrophages and virulence in mice. | the salmonella spvb protein possesses adp-ribosyl transferase activity. spvb, acting as an intracellular toxin, covalently modifies monomeric actin, leading to loss of f-actin filaments in salmonella-infected human macrophages. using defined salmonella mutants, different functional components of the spi-2 type three secretion system (ttss), ssav, spic, sseb, ssec, and ssed, were found to be required for spvb-mediated actin depolymerization in human macrophages. expression of spvb protein in salm ... | 2008 | 18248436 |
| coordinated regulation of expression of salmonella pathogenicity island 1 and flagellar type iii secretion systems by atp-dependent clpxp protease. | salmonella enterica serovar typhimurium delivers a variety of proteins via the salmonella pathogenicity island 1 (spi1)-encoded type iii secretion system into host cells, where they elicit several physiological changes, including bacterial invasion, macrophage apoptosis, and enteropathogenesis. once salmonella has established a systemic infection, excess macrophage apoptosis would be detrimental to the pathogen, as it utilizes macrophages as vectors for systemic dissemination throughout the host ... | 2008 | 18245288 |
| human salmonella clinical isolates distinct from those of animal origin. | the global trend toward intensive livestock production has led to significant public health risks and industry-associated losses due to an increased incidence of disease and contamination of livestock-derived food products. a potential factor contributing to these health concerns is the prospect that selective pressure within a particular host may give rise to bacterial strain variants that exhibit enhanced fitness in the present host relative to that in the parental host from which the strain w ... | 2008 | 18245251 |
| on-line monitoring of microbial volatile metabolites by proton transfer reaction-mass spectrometry. | a method for analysis of volatile organic compounds (vocs) from microbial cultures was established using proton transfer reaction-mass spectrometry (ptr-ms). a newly developed sampling system was coupled to a ptr-ms instrument to allow on-line monitoring of vocs in the dynamic headspaces of microbial cultures. the novel ptr-ms method was evaluated for four reference organisms: escherichia coli, shigella flexneri, salmonella enterica, and candida tropicalis. headspace vocs in sampling bottles con ... | 2008 | 18245241 |
| biofilm formation in field strains of salmonella enterica serovar typhimurium: identification of a new colony morphology type and the role of sgi1 in biofilm formation. | in this study we examined the extent of biofilm formation in field strains of salmonella enterica serovar typhimurium (s. typhimurium), an important foodborne pathogen. ninety-four field strains of s. typhimurium were tested for their ability to form biofilm and components contributing to its formation. most s. typhimurium strains were highly capable of biofilm formation except for strains of phage type dt2 originating from pigeons. the most efficient biofilm forming strains were those of phage ... | 2008 | 18242887 |
| vaccines and immunotherapy against pseudomonas aeruginosa. | a number of different vaccines and several monoclonal antibodies have been developed in the last decades for active and passive vaccination against the gram-negative opportunistic pathogen pseudomonas aeruginosa. these approaches include vaccine antigens such as lipopolysaccharide, surface polysaccharides, polysaccharide-protein conjugates, flagella, outer membrane proteins, pili, whole formalin-killed p. aeruginosa cells, live-attenuated p. aeruginosa and salmonella enterica strains expressing ... | 2008 | 18242792 |
| the range of influence between cattle herds is of importance for the local spread of salmonella dublin in denmark. | the objective of the study was to estimate the range of influence between cattle herds with positive salmonella dublin herd status. herd status was a binary outcome of high/low antibody levels to salmonella dublin in bulk-tank milk and blood samples collected from all cattle herds in denmark for surveillance purposes. two methods were used. initially, a spatial generalised linear mixed model was developed with an exponential correlation function to estimate the range of influence simultaneously ... | 2008 | 18242741 |
| using salmonella enterica serotype typhimurium to model intestinal fibrosis. | 2008 | 18242605 | |
| antimicrobial properties of milkfat globule membrane fractions. | milkfat globule membranes (mfgms) were prepared from bovine cream according to standard procedures. these membranes and peptide hydrolysates, which were generated by proteolysis with immobilized digestive enzymes, were screened for antibacterial activity against escherichia coli o157:h7, listeria monocytogenes, salmonella enterica typhimurium, pseudomonas fluorescens, bacillus cereus, lactobacillus acidophilus, and lactobacillus gasseri. assays were first performed on beef heart infusion (bhi) p ... | 2008 | 18236672 |
| microbiological profile of greenhouses in a farm producing hydroponic tomatoes. | produce, including tomatoes, has been implicated in several outbreaks of foodborne illness. a number of the sources of contamination for produce grown in open fields are known. however, as an alternative agricultural system, hydroponic greenhouses are reasonably expected to reduce some of these sources. the objective of the present study was to determine the microbiological profile of tomatoes grown in greenhouses at a mexican hydroponic farm with a high technological level and sanitary agricult ... | 2008 | 18236663 |
| an oral recombinant vaccine in dogs against echinococcus granulosus, the causative agent of human hydatid disease: a pilot study. | dogs are the main source of human cystic echinococcosis. an oral vaccine would be an important contribution to control programs in endemic countries. we conducted two parallel experimental trials in morocco and tunisia of a new oral vaccine candidate against echinococcus granulosus in 28 dogs. the vaccine was prepared using two recombinant proteins from adult worms, a tropomyosin (egtrp) and a fibrillar protein similar to paramyosin (ega31), cloned and expressed in a live attenuated strain of sa ... | 2008 | 18235847 |
| label-free detection of dna with interdigitated micro-electrodes in a fluidic cell. | we investigate the analytical performance of an interdigitated electrode sensor for the label-free detection of dna, by monitoring the complex impedance of 5 microm wide interdigitated pt microelectrodes on a glass substrate. we detect the hybridization of unlabeled 38-mer target ssdna with a complementary probe that is bound on the glass in between the electrodes by a disuccinimidyl terephtalate and aminosilane immobilization procedure. the sensor is mounted in a microfluidic flow cell, in whic ... | 2008 | 18231670 |
| characterisation of salmonella enterica serotype typhimurium isolates from wild birds in northern england from 2005 - 2006. | several studies have shown that a number of serovars of salmonella enterica may be isolated from wild birds, and it has been suggested that wild birds may play a role in the epidemiology of human and livestock salmonellosis. however, little is known about the relationship between wild bird s. enterica strains and human- and livestock- associated strains in the united kingdom. given the zoonotic potential of salmonellosis, the main aim of this study was to investigate the molecular epidemiology o ... | 2008 | 18230128 |
| role of enteric fever in ileal perforations: an overstated problem in tropics? | to determine the role of enteric fever in ileal perforations. | 2008 | 18227599 |
| high level ciprofloxacin resistance in salmonella enterica isolated from blood. | over the last few years, resistance to ciprofloxacin in salmonella enterica has become a global concern. the present study was undertaken to find out the susceptibility pattern of salmonella enterica isolates in our hospital. | 2008 | 18227598 |
| the yejabef operon of salmonella confers resistance to antimicrobial peptides and contributes to its virulence. | pathogenic micro-organisms have evolved many strategies to counteract the antimicrobial peptides (amps) that they encounter upon entry into host systems. these strategies play vital roles in the virulence of pathogenic micro-organisms. the salmonella enterica serovar typhimurium genome has a gene cluster consisting of yeja, yejb, yeje and yejf genes, which encode a putative atp-binding cassette (abc) transporter. our study shows that these genes constitute an operon. we deleted the yejf gene, wh ... | 2008 | 18227269 |
| distinct functional domains of the salmonella enterica wbap transferase that is involved in the initiation reaction for synthesis of the o antigen subunit. | wbap is a membrane enzyme that initiates o antigen synthesis in salmonella enterica by catalysing the transfer of galactose 1-phosphate (gal-1-p) onto undecaprenyl phosphate (und-p). wbap possesses at least three predicted structural domains: an n-terminal region containing four transmembrane helices, a large central periplasmic loop, and a c-terminal domain containing the last transmembrane helix and a large cytoplasmic tail. in this work, we investigated the contribution of each region to wbap ... | 2008 | 18227248 |
| in vitro model of colonization resistance by the enteric microbiota: effects of antimicrobial agents used in food-producing animals. | a bioassay was developed to measure the minimum concentration of an antimicrobial drug that disrupts the colonization resistance mediated by model human intestinal microbiota against salmonella invasion of caco-2 intestinal cells. the bioassay was used to measure the minimum disruptive concentrations (mdcs) of drugs used in animal agriculture. the mdcs varied from 0.125 microg/ml for some broad-spectrum antimicrobial drugs (e.g., streptomycin) to 16 microg/ml for drugs with limited spectra of an ... | 2008 | 18227184 |
| braun lipoprotein (lpp) contributes to virulence of yersiniae: potential role of lpp in inducing bubonic and pneumonic plague. | yersinia pestis evolved from y. pseudotuberculosis to become the causative agent of bubonic and pneumonic plague. we identified a homolog of the salmonella enterica serovar typhimurium lipoprotein (lpp) gene in yersinia species and prepared lpp gene deletion mutants of y. pseudotuberculosis ypiii, y. pestis kim/d27 (pigmentation locus minus), and y. pestis co92 with reduced virulence. mice injected via the intraperitoneal route with 5 x 10(7) cfu of the deltalpp kim/d27 mutant survived a month, ... | 2008 | 18227160 |
| molecular evolution of salmonella enterica serovar typhimurium and pathogenic escherichia coli: from pathogenesis to therapeutics. | salmonella enterica serovar typhimurium (s. typhimurium) and certain escherichia coli are human pathogens that have evolved through the acquisition of multiple virulence determinants by horizontal gene transfer. similar genetic elements, as pathogenicity islands and virulence plasmids, have driven molecular evolution of virulence in both species. in addition, the contribution of prophages has been recently highlighted as a reservoir for pathogenic diversity. characterization of horizontally acqu ... | 2008 | 18226587 |
| isolation of avian strains of salmonella enterica serovar typhimurium from cats with enteric disease in the united kingdom. | 2008 | 18223269 | |
| virulent salmonella enterica infections can be exacerbated by concomitant infection of the host with a live attenuated s. enterica vaccine via toll-like receptor 4-dependent interleukin-10 production with the involvement of both trif and myd88. | during systemic disease in mice, salmonella enterica grows intracellularly within discrete foci of infection in the spleen and liver. in concomitant infections, foci containing different s. enterica strains are spatially separated. we have investigated whether functional interactions between bacterial populations within the same host can occur despite the known spatial separation of the foci and independence of growth of salmonellae residing in different foci. in this study we have demonstrated ... | 2008 | 18217948 |
| disinfectant test against monoculture and mixed-culture biofilms composed of technological, spoilage and pathogenic bacteria: bactericidal effect of essential oil and hydrosol of satureja thymbra and comparison with standard acid-base sanitizers. | to assess the antimicrobial action of three natural-derived products (essential oil, decoction and hydrosol of satureja thymbra) against biofilms, composed of useful, spoilage and pathogenic bacteria (formed as monoculture or/and mixed-culture), and to compare their efficiency with three standard acid and alkaline chemical disinfectants. | 2008 | 18217930 |
| human multidrug-resistant salmonella newport infections, wisconsin, 2003-2005. | we conducted a retrospective study of salmonella newport infections among wisconsin residents during 2003-2005. multidrug resistance prevalence was substantially greater in wisconsin than elsewhere in the united states. persons with multidrug-resistant infections were more likely than persons with susceptible infections to report exposure to cattle, farms, and unpasteurized milk. | 2007 | 18217570 |
| energy source of flagellar type iii secretion. | bacterial flagella contain a specialized secretion apparatus that functions to deliver the protein subunits that form the filament and other structures to outside the membrane. this apparatus is related to the injectisome used by many gram-negative pathogens and symbionts to transfer effector proteins into host cells; in both systems this export mechanism is termed 'type iii' secretion. the flagellar secretion apparatus comprises a membrane-embedded complex of about five proteins, and soluble fa ... | 2008 | 18216859 |
| distinct roles of the flii atpase and proton motive force in bacterial flagellar protein export. | translocation of many soluble proteins across cell membranes occurs in an atpase-driven manner. for construction of the bacterial flagellum responsible for motility, most of the components are exported by the flagellar protein export apparatus. the flii atpase is required for this export, and its atpase activity is regulated by flih; however, it is unclear how the chemical energy derived from atp hydrolysis is used for the export process. here we report that flagellar proteins of salmonella ente ... | 2008 | 18216858 |
| clinical response and outcome of infection with salmonella enterica serotype typhi with decreased susceptibility to fluoroquinolones: a united states foodnet multicenter retrospective cohort study. | patients with typhoid fever due to salmonella enterica serotype typhi strains for which fluoroquinolones mics are elevated yet that are classified as susceptible by the current interpretive criteria of the clinical and laboratory standards institute may not respond adequately to fluoroquinolone therapy. patients from seven u.s. states with invasive salmonella serotype typhi infection between 1999 and 2002 were enrolled in a multicenter retrospective cohort study. patients infected with salmonell ... | 2008 | 18212096 |
| binding specificity of salmonella plasmid-encoded fimbriae assessed by glycomics. | the salmonella enterica serotype typhimurium (s. typhimurium) genome encodes 12 intestinal colonization factors of the chaperone/usher fimbrial assembly class; however, the binding specificity is known for only one of these adhesins, known as type 1 fimbriae. here we explored the utility of glycomics to determine the carbohydrate binding specificity of plasmid-encoded fimbriae from s. typhimurium. a cosmid carrying the pef operon was introduced into escherichia coli and expression of fimbrial fi ... | 2008 | 18211897 |
| enzyme-assisted processing increases antimicrobial and antioxidant activity of bilberry. | the effects of nine cell wall-degrading enzymes on the antimicrobial and antioxidant activities of bilberry were studied. antimicrobial activity was measured using the human pathogens salmonella enterica sv. typhimurium and staphylococcus aureus as test strains. enzyme treatments liberated phenolics from the cell wall matrix, which clearly increased the antimicrobial activity of berry juices, press cakes, and berry mashes on the basis of plate counts. antibacterial effects were stronger against ... | 2008 | 18211029 |
| survival of enteric microorganisms on grass surfaces irrigated with treated effluent. | treated effluent can be reused for the irrigation of parks and sports grounds but there is an associated potential public health risks from microbial pathogens present on the grass surface, particularly when used for contact sports. the main aim of this study was to investigate the survival of pathogenic and indicator microorganisms on the grass surface of a sports ground irrigated with treated effluent under differing climatic conditions. results showed that salmonella enterica serotype typhimu ... | 2008 | 18209287 |
| inactivation kinetics of inoculated escherichia coli o157:h7 and salmonella enterica on lettuce by chlorine dioxide gas. | the purpose of this investigation was to study inactivation kinetics of inoculated escherichia coli o157:h7 and salmonella enterica on lettuce leaves by clo(2) gas at different concentrations (0.5, 1.0, 1.5, 3.0, and 5.0 mg l(-1)) for 10 min and to determine the effect of clo(2) gas on the quality and shelf life of lettuce during storage at 4 degrees c for 7 days. one hundred microliters of each targeted organism was separately spot-inoculated onto the surface (5 cm(2)) of lettuce (approximately ... | 2008 | 18206766 |
| role of mitogen-activated protein kinases in the inos production and cytokine secretion by salmonella enterica serovar typhimurium porins. | the expression of inducible nitric oxide synthase (inos) is a critical factor in both physiological and pathological functions. the present study examined the role of mitogen-activated protein kinases (mapks) in the regulation of inos and proinflammatory cytokine production in raw 264.7 cells in response to salmonella enterica serovar typhimurium porins. by use of western blotting for inos detection and enzyme-linked immunosorbent assay (elisa) for quantization of cytokine secretion, selective p ... | 2008 | 18206384 |
| flagellin is required for salmonella-induced expression of heat shock protein hsp25 in intestinal epithelium. | flagellin is a bacterial protein responsible for activation of toll-like receptor 5 (tlr5), which we hypothesize is involved in salmonella's induction of cytoprotective heat shock proteins in intestinal epithelial cells. flagellin induces the cytoprotective heat shock protein hsp25 in different intestinal epithelial cell lines and in mouse intestine. flagellin induces hsp25 expression in a time-dependent manner in vitro. this effect is transcriptional, as confirmed by luciferase reporter assays ... | 2008 | 18202113 |
| peptide-assisted degradation of the salmonella mgtc virulence factor. | mgtc is a virulence factor common to several intracellular pathogens that is required for intramacrophage survival and growth in magnesium-depleted medium. in salmonella enterica, mgtc is coexpressed with the mgtb magnesium transporter and transcription of the mgtcb operon is induced by magnesium deprivation. despite the high level of mgtcb transcriptional induction in magnesium-depleted medium, the mgtc protein is hardly detected in a wild-type salmonella strain. here, we show that downregulati ... | 2008 | 18200043 |
| the mcdonald-kreitman test and slightly deleterious mutations. | it is possible to estimate the proportion of substitutions that are due to adaptive evolution using the numbers of silent and nonsilent polymorphisms and substitutions in a mcdonald and kreitman-type analysis. unfortunately, this estimate of adaptive evolution is biased downward by the segregation of slightly deleterious mutations. it has been suggested that 1 way to cope with the effects of these slightly deleterious mutations is to remove low-frequency polymorphisms from the analysis. we inves ... | 2008 | 18195052 |
| toll-like receptor 5-deficient mice have dysregulated intestinal gene expression and nonspecific resistance to salmonella-induced typhoid-like disease. | the recognition of flagellin by toll-like receptor 5 (tlr5) is the dominant means by which model intestinal epithelia activate proinflammatory gene expression in response to salmonella enterica. the role of the flagellin-tlr5 interaction in vivo has been addressed primarily via studies that use flagellar mutants. such studies suggest that host recognition of flagellin promotes rapid neutrophil recruitment that protects the host from this pathogen. however, these works do not directly address the ... | 2008 | 18195036 |
| aggregation via the red, dry, and rough morphotype is not a virulence adaptation in salmonella enterica serovar typhimurium. | the salmonella rdar (red, dry, and rough) morphotype is an aggregative and resistant physiology that has been linked to survival in nutrient-limited environments. growth of salmonella enterica serovar typhimurium was analyzed in a variety of nutrient-limiting conditions to determine whether aggregation would occur at low cell densities and whether the rdar morphotype was involved in this process. the resulting cultures consisted of two populations of cells, aggregated and nonaggregated, with the ... | 2008 | 18195033 |
| biosynthesis and iroc-dependent export of the siderophore salmochelin are essential for virulence of salmonella enterica serovar typhimurium. | in response to iron deprivation, salmonella enterica serovar typhimurium secretes two catecholate-type siderophores, enterobactin and its glucosylated derivative salmochelin. although the systems responsible for enterobactin synthesis and acquisition are well characterized, the mechanisms of salmochelin secretion and acquisition, as well as its role in salmonella virulence, are incompletely understood. herein we show by liquid chromatography-mass spectrometry analysis of culture supernatants fro ... | 2008 | 18194158 |
| molecular characterization of the salmonella enterica serovar typhi vi-typing bacteriophage e1. | some bacteriophages target potentially pathogenic bacteria by exploiting surface-associated virulence factors as receptors. for example, phage have been identified that exhibit specificity for vi capsule producing salmonella enterica serovar typhi. here we have characterized the vi-associated e1-typing bacteriophage using a number of molecular approaches. the absolute requirement for vi capsule expression for infectivity was demonstrated using different vi-negative s. enterica derivatives. the p ... | 2008 | 18192390 |
| diseases and reproductive success in a wild mammal: example in the alpine chamois. | density-dependent and climatic factors affect reproduction and dynamics of wild ungulates. parasites can also decrease reproductive success through either a direct abortive effect or a negative impact on host growth and body condition. however, few studies have investigated the effect of parasitism on fecundity of ungulates in natural conditions. we studied three bacterial infections caused by salmonella enterica serovar abortusovis, chlamydophila abortus and coxiella burnetii. these bacteria ar ... | 2008 | 18189146 |
| transduction of bla(cmy-2), tet(a), and tet(b) from salmonella enterica subspecies enterica serovar heidelberg to s. typhimurium. | antimicrobial resistance of salmonella spp., especially resistance mediated by extended spectrum beta-lactamases (esbl), is a growing public health concern. understanding the mechanisms through which salmomella spp. acquire the resistance genes can lead to the development of intervention and mitigation strategies. thirty-one salmonella isolates of bovine origin were analyzed by serotyping, antimicrobial susceptibility testing, phage induction and bacterial host range determination, and phage tra ... | 2008 | 18187273 |
| comparison of antimicrobial resistance genes in nontyphoidal salmonellae of serotypes enteritidis, hadar, and virchow from humans and food-producing animals in england and wales. | isolates of salmonella enterica serovars enteritidis (n = 17), hadar (n = 18), and virchow (n = 13) from cases of human infection and from food production animals were screened using a miniaturized antimicrobial microarray to determine the number and spectra of resistance genes. among enteritidis, the number of genes detected was: animal isolates, mean = 4.6; human isolates, mean = 5.3. resistance to streptomycin, trimethoprim, and sulfonamides was usually encoded by only one resistance gene in ... | 2007 | 18184054 |
| laboratory-based salmonella surveillance in fiji, 2004-2005. | although foodborne diseases are an important public health problem worldwide, the burden of foodborne illness is not well described in most pacific island countries and territories. laboratory-based surveillance programs can detect trends and outbreaks, estimate burden of illness, and allow subtyping of enteric pathogens (e.g. salmonella serotyping), which is critical for linking illness to food vehicles and animal reservoirs. to enhance public health capacity in fiji for foodborne disease surve ... | 2005 | 18181494 |
| induction of the carrier state in pigeons infected with salmonella enterica subspecies enterica serovar typhimurium pt99 by treatment with florfenicol: a matter of pharmacokinetics. | paratyphoid caused by salmonella enterica subsp. enterica serovar typhimurium is the main bacterial disease in pigeons. the ability of salmonella serovar typhimurium to persist intracellularly inside pigeon macrophages results in the development of chronic carriers, which maintain the infection in the flock. in this study, the effect of drinking-water medication with florfenicol on salmonella infection in pigeons was examined. the pharmacokinetics of florfenicol in pigeons revealed a relatively ... | 2008 | 18180355 |