Publications

TitleAbstractYear
Filter
PMID(sorted descending)
Filter
different sensitivity of the sarcoplasmic reticulum ca(2+)-atpase enzyme to fluorescein-isothiocyanate in rabbit and carp muscles.1. carp and rabbit sarcoplasmatic reticulum ca(2+)-atpase enzymes were compared with respect to their sensitivity to fitc labelling. 2. the carp enzyme showed much lower sensitivity to fitc in the ca(2+)-mg2+ activated atpase activity. fifty percent inhibition was observed at 20 microm labelling fitc concentration; in rabbit enzyme this inhibition was already achieved at 2 microm fitc. 3. the tryptic cleavage products of the carp enzyme identified with immunoblot analysis as well as with fitc fl ...19921388116
effects of polyoxyethylene (20) sorbitan monooleate on the acute toxicity of linear alkylbenzenesulfonate (c12las) to fish.effects of polyoxyethylene (20) sorbitan monooleate (smoe20) on the acute toxicity of linear alkylbenzenesulfonate (c12las) to fish were investigated in red killifish (orizias latipes) and carp (cyprinus carpio). by adding polyoxyethylene sorbitan ester to c12las solution, the acute toxicity of c12las decreased. as a decreasing toxic effect of these nonionic surfactants, the depression of gill damage was histopathologically observed. no significant hematological and blood biochemical differences ...19921385076
the effect of malathion, diazinon, and various concentrations of zinc, copper, nickel, lead, iron, and mercury on fish.acute and chronic toxicity tests for malathion, diazinon, copper (cu), mercury (hg), lead (pb), zinc (zn), nickel (ni), and iron (fe) were conducted. mortalities of barilius vagra and cyprinus carpio (common carp) were variable but lc50-96 hr were similar for pesticides. adult b. vagra seem to be more sensitive to malathion than juvenile carp. both juvenile carp and adult b. vagra were extremely sensitive to diazinon. long-term exposure to pesticides modified morphology and behavior. the lc50-96 ...19921384613
neuropeptides in the torus semicircularis of the carp (cyprinus carpio).the distribution of fibers and cell bodies containing neurotensin, neurokinin a, galanin, or somatostatin-28(1-12) immunoreactivity in the torus semicircularis of the carp was studied using an indirect immunoperoxidase technique. in this mesencephalic region, a high-density of galanin-immunoreactive fibers was found, whereas neurokinin a or somatostatin-28(1-12)-immunoreactive processes were observed at a moderate density and neurotensin-immunoreactive fibers at a low density. cell bodies contai ...19921377585
the effect of pesticides on carp (cyprinus carpio l). acetylcholinesterase and its biochemical characterization.the activity and molecular forms of acetylcholinesterase (ache) were characterized in tissues of the carp (cyprinus carpio). tissue ache activity was determined in response to specific inhibitors (ethopropazine, bw 284 c51) or pesticides (cuso4, paraquat (pq), methidathion (md)). the highest ache activity was found in the serum (878 +/- 100 u/liter), followed by the brain (113 +/- 12 u/liter), heart (89 +/- 6 u/liter), and trunk muscle (35 +/- 5 u/liter). experiments with specific choline estera ...19921375147
organization and nucleotide sequence of carp gonadotropin alpha subunit genes.we have used pcr to amplify and align the sequence of two genes encoding cgth alpha. both genes comprise four exons and three introns. the organization of cgth alpha genes is very similar to that of mammalian gth alpha genes. however, the cgth alpha genes only span a region of 1.2 kb which is much smaller than those mammalian gth alpha genes.20031370380
gene expression following transfection of fish cells.various genes containing different transcriptional regulatory elements (tre) and the bacterial marker gene coding for chloramphenicol acetyl transferase were transfected into several fish cell lines to evaluate the efficiency of expression in comparison with mammalian cells. the cmv and rsv tre were the most efficient non-inducible promoters in directing reporter gene expression. rsv and cmv appeared of similar potency in a stable fish cell line. the human hsp-70 promoter showed high potency in ...19921369156
development of expression vectors for transgenic fish.genetic alteration of fish is important for aquatic biotechnology as well as for investigating molecular interactions that occur during vertebrate development. the numerous, large, transparent, and externally fertilized eggs of many fish species make them ideally suitable for genetic manipulation, especially for production of transgenic animals. genetic engineering of fish requires suitable expression vectors. accordingly, we developed two fish expression vectors, fv-1 and fv-2, which contain th ...20051366961
an initial screen of a series of neuroactive peptides for activity on identified central neurones of helix aspersa.1. intracellular recordings were made from identified neurones in the suboesophageal ganglia of helix aspersa. seven neuropeptides were tested for activity and their actions compared with acetylcholine and fmrfamide. 2. three peptides isolated from nematodes, af-1, af-2 and pan-1 had mainly inhibitory effects with thresholds of around 1 nm. this inhibition was due to an increase in potassium conductance. 3. the molluscan neuropeptides lssfvriamide, carp and acep-1 were all active on certain neur ...19921363295
effects of hypoxia on renal function in carp, cyprinus carpio.1. changes in urine and blood properties and heart rate (hr) of carp were investigated during and following hypoxia. 2. when carp were exposed to hypoxic conditions, urine flow in some carp increased immediately. however, it decreased gradually with time. osmotic pressure and na+, cl-, ca2+ and mg2+ levels in urine increased in contrast to urine flow. k+, p, ammonium, and lactic acid levels in urine increased gradually. 3. when carp lost their balance, blood ph and plasma k+ were lower, and rbc, ...19921351452
xenobiotic-metabolizing enzyme systems in test fish--iv. comparative studies of liver microsomal and cytosolic hydrolases.1. the initial slopes of the substrate-activity curves of several hydrolases were determined in the microsomal and cytosolic fractions of the liver of several fish recommended by oecd for the regulatory testing of chemicals. 2. inter-species differences ranged within a factor of 7-17 for the esterases and reached a factor of 60 for the amidase. guppy and carp appeared endowed with hydrolase activities which, overall, are much higher than zebra fish, trout and golden orfe. 3. the comparison with ...19921350956
immunochemical characterization of rat testicular asparagine synthetase.we studied immunochemical properties of rat testicular asparagine synthetase. western blot analysis of testis extract with polyclonal antibody raised against purified asparagine synthetase revealed an immunoreactive band at 62 kda. the pancreas, brain, thymus, and spleen also showed 62-kda bands. the intensities of these bands were roughly proportional to the specific activities of the enzyme in these tissues. the antibody showed some degree of cross-reactivity to asparagine synthetases from hum ...19921349469
mechanisms for anoxic survival in the vertebrate brain.when energy supplies to the mammalian brain are significantly reduced by anoxia, for a very short time energy requirements are curtailed by such routes as the suppression of synaptic transmission, while energy supply is enhanced by an increase in cerebral blood flow and an increase in glycolysis. the reduction of atp consumption is insufficient to match the greatly curtailed supply of atp coming from anaerobic glycolysis and the hydrolysis of pcr, and within minutes atp falls, there is a loss of ...19921348613
oral absorption of biologically active insulin in common carp (cyprinus carpio l.).1. bovine insulin dissolved in 0.05 m deoxycholic acid was absorbed through oral intubation in fish weighing 200-300 g. 2. the peak of absorption appeared in all fish, 30-45 min after intubation and was followed by a gradual decrease. the extent of absorption was extremely variable, with up to a 20-fold difference between individuals. no detectable insulin was found in fish intubated with vehicle. 3. the absorbed hormone retained 17-88% of its lipogenic bioactivity in vitro. the absorbed insulin ...19921347729
[production of a carp-based hamburger-like product by reducing the water activity].the experimental conditions were determined in order to conserve lean fish by means of combined factors based on aw and ph reduction as well as the addition of an antifungal. theoretical aw was determined in formulas containing fish, sodium chloride, glycerol and sorbitol applying a mathematic model. from the results of the prediction, 4 formulas were prepared experimentally with (cyprinus carpio). phosphoric acid was added to the products in order to obtain a 5.5-6.0 ph. the final formulas were ...19921341858
switches in fish myosin genes induced by environment temperature in muscle of the carp.fish are cold blooded animals and their muscle function is expected to be greatly affected by environmental temperature. species that live in the antarctic ocean have evolved a different contractile system to fish that live in the tropical waters. in the case of antarctic fish they have a higher specific myofibrillar atpase activity but 'the trade off' seems to be a lower thermal stability. they are thus capable of a greater muscle power output at low temperatures but the lower thermal stability ...19921341032
electroporation: a method for transferring genes into the gametes of zebrafish (brachydanio rerio), channel catfish (ictalurus punctatus), and common carp (cyprinus carpio).recombinant plasmids containing the rous sarcoma virus long-terminal repeat (rsvltr) promoter linked to either rainbow trout (oncorhyncus mykiss) growth hormone 1 (rtgh1) or growth hormone 2 (rtgh2) cdna were linearized and introduced into the fertilized eggs of zebrafish (brachydanio rerio), channel catfish (ictalurus punctatus), and common carp (cyprinus carpio) by both electroporation and microinjection. the latter two species had these rainbow trout constructs (rsvltr-rtgh1cdna or rsvltr-rtg ...19921339228
low concentrations of neuroactive peptides modulate cholinergic transmission and cyclic amp levels in helix aspersa.1. fmrfamide and the catch relaxing peptide (carp) at 0.01-0.1 nm modulate acetylcholine (ach) induced currents of identified neurons of helix aspersa, but have no direct effect on membrane current and conductivity. 2. both fmrfamide and carp noncompetitively inhibit ach cl- responses while having either no effect or increasing ach na+/k+ response. 3. the inhibitory effect of fmrfamide and carp on the ach cl- response was eliminated following pretreatment with forskolin (20 microm), an activator ...19921338559
dopamine decreases receptive field size of rod-driven horizontal cells in carp retina.receptive field size of rod-driven horizontal cells (hcs) in the carp retina was measured by the spread of responses to the slit of light stimulus with changing the distance from the recording electrode and it was found to decay with a single exponential function. by perfusing 10 microm dopamine (da) the length constant of rod-driven hcs was reduced to half and the response amplitude in the centre increased approximately two-fold, and the input resistance was markedly increased. this suggests th ...19921337637
[the characteristics of carbohydrate transport in the intestines of fresh-water fishes].the maximal level of carbohydrate transport was found in the proximal portion of the intestine and in the distal one in the bream and carp. after 48-hr starvation the carbohydrate transport was decreased along the whole length of the intestine. anoxia and some inhibiting agents changed the intensity of glucose and galactose transport in the fish intestine.19921335912
the effects of atp, inorganic phosphate, protons, and lactate on isolated myofibrillar atpase activity.the purpose of this study was to examine the effects of lactate, protons, inorganic phosphate, and atp on myofibrillar atpase activity. myofibrils were isolated from carp (cyprinius carpio l.) fast-twitch white muscle, and myofibrillar atpase activities were assessed under maximal activating calcium levels (pca 4.0) at 10 degrees c in reaction media containing metabolic profiles similar to those seen in fatiguing muscles. the ca(2+)-activated atpase activity was assessed by an atp regenerating a ...19921335356
phototransduction in cones as examined in excised membrane patch.phototransduction mechanisms have been so far investigated mostly in rods, whereas those in cones are much less known. in the present experiment, we investigated phototransduction mechanisms in inside-out patches excised from cone outer segments of the carp. cyclic gmp-activated channels on the patch became light-sensitive when both gtp and mg2+ were supplied by perfusion. when the channels were activated by a hydrolysis-resistant analogue of cgmp, activities were not suppressed by light even th ...19921331581
characterization of gonadotropin-releasing hormone (gnrh) receptors in the ovary of common carp (cyprinus carpio).gonadotropin-releasing hormone (gnrh) binding sites have been characterized in the fully mature common carp ovary, using an analog of salmon gnrh ([d-arg6,trp7,leu8,pro9-net]-gnrh; sgnrh-a) as a labeled ligand. binding of sgnrh-a to carp follicular membrane preparation was found to be time-, temperature-, and ph-dependent. optimal binding was achieved after 40 min of incubation at 4 degrees c at ph 7.6; binding was found to be unstable at room temperature. binding of radioligand was a function o ...19921325864
[effects of prolonged darkness and background illumination on cone horizontal cells in carp retina: a correlative study of morphology and physiology].using intact, immobilized carp preparations, changes in light responsiveness of cone horizontal cells and ultrastructures of their terminals in cone pedicles (hcts) were correlatively examined in prolonged (greater than 2 h) darkness and after the presentation of a dim background light. following background illumination cone horizontal cells exhibit high light responsiveness and hcts give rise to a lot of long, fingerlike or ball-like extensions, called slender or round spinules. when the retina ...19921320293
m-phase-specific histone h1 kinase in fish oocytes. purification, components and biochemical properties.we demonstrate, for the first time in fish, that a ca(2+)-independent and cyclic-nucleotide-independent histone h1 kinase activity oscillates according to the cell cycle of the oocyte, peaking at the first and the second meiotic metaphase with a transient drop between them. the kinase, m-phase-specific histone h1 kinase (m-h1k), was purified from mature carp oocytes by using two exogenous substrates for assaying its activity: histone h1 and a synthetic peptide (sp peptide, kkaakspkkakk) containi ...19921315270
selection of promoters for gene transfer into fish.a variety of gene constructs containing carp beta-actin regulatory sequences were tested for their ability to drive transient expression of the chloramphenicol acetyltransferase reporter gene in 3 fish cell lines: carp epithelial cells (epc), rainbow trout hepatoma cells (rth149), and rainbow trout fibroblasts (rtg2). the constructs showed a wide variation in their levels of expression, and there were significant differences in the effects of transcriptional elements in the 3 cell lines. sequenc ...20081308823
fish transgene expression by direct injection into fish muscle.the ability of a promoter sequence to drive expression of a reporter gene can be determined by direct injection of copies of the cloned sequence into fish muscle, followed by biopsy of muscle from the site of injection. we describe a set of experiments in which copies of the constructs fv1 and fv2, both comprising a carp beta-actin promoter sequence spliced to the bacterial reporter gene cat, were injected into the muscle of tilapia fish )oreochromis niloticus) of between 5 and 8 cm body length. ...19921308819
introducing foreign genes into fish eggs with electroporated sperm as a carrier.a new method has been developed for introduction of foreign genes into fish eggs. the procedure is based on the incubation of fish sperm cells suspended in dilute citrate solution with plasmid dna, followed by application of high-field-strength electrical pulses (electroporation) to increase dna binding., uptake, or both. tissue homogenates and genomic dna extracts of free swimming fry developed from eggs fertilized with treated sperm was tested to evaluate the efficiency of gene transfer. dot b ...20121308817
isolation and sequence analysis of carp gonadotropin beta-subunit gene.using the cdna encoding the beta subunit of carp gonadotropin (cgth-beta) as a probe, 14 clones containing cgth-beta gene have been isolated from a carp genomic library. nucleotide sequence analysis indicated that the transcriptional unit of the cgth-beta gene is 1.2 kb. similar to mammalian gth-beta genes, cgth-beta gene contains three exons and two introns. the locations of the exon/intron junctions also correspond to those of mammalian gth-beta gene. using the primer extension assay, the star ...19921308812
chemical identification of catfish growth hormone and prolactin.isolation and primary structure of growth hormone (gh) and prolactin (prl) from the pituitary gland of catfish (ictalurus punctatus) are described. alkaline extract of the pituitary glands was fractionated by gel filtration on sephadex g-75, ion-exchange chromatography on deae-cellulose, and reversed-phase high-performance liquid chromatography on octadecyl silica ods. catfish gh and prl were identified by western blotting with antisera against chum salmon gh and prl. the catfish gh consists of ...19921308206
protease activities in carp retina.protease of carp retina were examined by electrophoresis and fluorogenic assays. a 70 kd serine protease with an alkaline ph optimum was detected in gelatin-containing polyacrylamide gels. a similar enzyme was found in carp brain and muscle, but not in lens. using aminomethylcoumarin (mca) substrates, activities that hydrolysed z-phe-arg-mca, boc-ala-gly-pro-arg-mca and various aminoacyl-mcas were detected. the z-phe-arg-mca hydrolase was an acidic cysteine protease, whereas the boc-ala-gly-pro- ...19921303734
peptidergic regulation of muscle movements in a prosobranch mollusc, rapana thomasiana.peptidergic regulation of the contraction of radula muscles and the heart beat in a prosobranch mollusc, rapana thomasiana was investigated. in the radula muscles, fmrfamide-related peptides were suggested to act on presynaptic sites to enhance their contraction elicited by nerve stimulation, possibly by increasing the release of a main transmitter. a catch-relaxing peptide (carp) was demonstrated to depress the contraction by acting on postsynaptic sites. in the heart, fmrfamide enhanced the am ...19921299108
[purification and characterization of hec toxin produced by aeromonas hydrophila].an extracellular toxin produced by aeromonas hydrophila from cultured crucian carp with septicemia was detected. the toxin was purified by ammonium sulfate precipitation, deae-cellulose chromatography and sephadex g-100 gel filtration. the factor was a single polypeptide with a molecular weight of 52.5kd determined by sds-page. the heat-stable toxin possesses hemolytic, enterotoxic and cytolytic activities. the hemolytic activity on human erythrocytes was 3.81 x 10(3) hu/mg, cd50 for vero cell w ...19921299032
deletion 11q23-->qter (jacobsen syndrome). report of three new patients.three unrelated patients with de novo del 11q23-->qter are reported. clinical features included growth and mental retardation, hypotonia, trigonocephaly, facial dysmorphism with hypertelorism, epicanthal folds, abnormally shaped palpebral fissures, eye globe malformations, depressed nasal bridge, "carp-shaped" mouth, highly arched palate, low set and malformed ears. one patient had congenital heart defect, and reduced platelet count. this syndrome, originally reported by jacobsen, is now corrobo ...19921296516
cutaneous graft-versus-host-like reaction after paternal lymphocyte immunization for prevention of recurrent abortion.we have presented an immunocompetent patient who, after paternal lymphocyte immunization, developed a gvh-like skin reaction; she recovered spontaneously. this complication is rarely seen. this was the only case observed in our series of over 70 patients immunized. a similar phenomenon was not reported in a larger series by carp and colleagues (1). however, the case deserves special attention because this reaction can be life-threatening. therefore, we suggest that paternal lymphocyte immunizati ...19921290481
light chains of carp and pike skeletal muscle myosins. isolation and characterization of the most anodic light chain on alkaline ph electrophoresis. 19761278404
biosynthesis of fatty acids by the carp, cyprinus carpio l., in relation to environmental temperature.incorporation in vivo of sodium 1-14c-acetate into different lipid classes and fatty acids of total lipids and phospholipids of warm adapted and cold adapted carp livers was studied at 5 c and 22 c, respectively. the fatty acid composition of total lipids and phospholipids was also determined. the level of long chain polyunsaturated fatty acids in both total lipid and phospholipid fraction was higher in cold adapted fish than in warm adapted ones. the distribution of radioactivity among differen ...19761271977
heart rate and locomotor activity in fish: correlation and circadian and circannual differences in cyprinus carpio l. 19761269651
distribution of selected metals in tissue sample of carp, cyprinus carpio. 19761268353
[evoked potentials in the optic tectum of the carp brain upon electric stimulation of the facial nerve].the evoked potentials in the carp tectum opticum in response to stimulation of the afferent pathway for chemical nonolfactory reception, the facial nerve, are positive deflections of 5-25 ms latency which do not reverse during microelectrode submersion. according to the evoked potential amplitude and latent period 7 active zones were distingnished in the ipsilateral tectum differing from each other either in one or in both parameters. the dependence of the response evoked by facial nerve stimula ...19761264291
a somatotopic and functional representation of the respiratory muscles in the trigeminal and facial motor nuclei of the carp (cyprinus carpio l.).the place of the cells innervating six most important respiratory muscles in the carp (cyprinus carpio l.) were determined by means of the horseradish peroxidase technique. it was found that these muscles are represented in the rostral and caudal subdivisions of the trigeminal and facial motor nuclei. all connections were ipsilateral. the distribution of the various cell populations over the different subnuclei has been related to the function of the respective muscles in the respiratory cycle. ...19761262554
effects of calcitonin and ultimobranchialectomy (ubx) on calcium and bone metabolism in the eel, anguilla anguilla l.prolonged administration of synthetic salmon calcitonin (sct) to immature female silver eels, maintained in sea water, provoked a decrease of the serum calcium concentration and an increase of both the osteoblastic apposition and of the degree of mineralization of the intercellular matrix in the vertebral bone. the osteoclastic resorption and osteocytic osteolysis were not significantly affected, however the osteoclastic index was reduced. the ultimobranchial body, site of ct secretion, was caut ...19761260486
polymorphism of transferrin in carp (cyprinus carpio l.): genetic determination, isolation, and partial characterization.seven transferrin variants (a,b,c,d,e,f, and g) have been found in carp sera (cyprinus carpio l.). genetic analysis involves five variants and agrees with the hypothesis of simple codominant autosomal inheritance at one transferrin (tf) locus in spite of the fact that the carp is a tetraploid in relation to other species of the same family. carp populations from three regions were studied which differed in gene frequencies. individual populations were in hardy-weinberg equilibrium. the polymorph ...19761259703
re-examination of horizontal cells in the carp retina with procion yellow electrode. 19761258385
stimulation of carp interrenal function by adrenocorticotrophin.the effect of acth on cortisol concentration in the plasma of the carp has been studied; cortisol was determined using a competitive protein-binding radioassay. blood was sampled at 12.00 h (the diurnal minimum) by cardiac puncture. the plasma cortisol level of undisturbed carp (blood obtained 1--3 min after netting) was 16 +/- 14 (s.e.m.) ng/ml. one hour after injection of acth (1-0 i.u./100 g body wt., i.m.) the level of cortisol increased to 415 +/- 62 ng/ml and reached a maximum of 656 +/- 6 ...19761255049
two-way selection for growth rate in the common carp (cyprinus carpio l.)the domesticated european carp was subjected to a two-way selection for growth rate. five generations of mall selection for faster growth rate did not yield any response, but subsequent selection between groups (families) resulted in considerable progress while maintaining a large genetic variance. selection for slow growth rate yielded relatively strong response for the first three generations. random-bred control lines suffered from strong inbreeding depression and when two lines were crossed, ...19761248737
ultrastructural aspects of mature cyprinus carpio erythrocytes. 19751242080
[effects of transections and electrical coagulations in the medulla oblongata upon the activities in the respiratory muscles of the crucian carp (author's transl)].the following conclusions may be drawn from the results in this work. the respiratory cycles are formed by the neuronal machinery in the reticular formation under the posterior part of the vagal motor nucleus. the motor neurones or the neuronal networks composing the motor nucleus of the respiratory muscles tonically discharge the action potentials, when the neurones or the networks are released from the inhibitory influences of the interneurones connecting the neuronal machinery to the motor ne ...19751238557
terbium replacement of calcium in carp muscle calcium-binding parvalbumin: an x-ray crystallographic study. 19751237627
quantification of phase improvement in the tangent formula application to carp muscle calcium-binding protein. 19751237626
refinement of the structure of carp muscle calcium-binding parvalbumin by model building and difference fourier analysis. 19751237625
[electromyographical studies on the swimming movement of carp. ii. pectoral fin movement (author's transl)]. 19751237055
[morphofunctional types of synapses in the neurosecretory cells of the carp preoptic nucleus].the ultrastructure of synaptic endings of the neurosecretory cells of the nucleus preopticus was examined in adult cyprinus carpio l. two of synpatic endings occur: type i--small agranular vesicles and large granular vesicles, type ii--only agranular vesicles. the functioning of the nucleus preopticus neurosecretory cells in cyprinus carpio l is presumably controlled by the synpatic endings of the adrenergic (synaptic endings of type i) as well as of the cholinergic (synaptic endings of type ii) ...20051231093
calcium binding sites of rabbit troponin and carp parvalbumin. 19751225590
on the origin, development and probable functions of taste bud in the lip and bucco-pharyngeal epithelia of an indian freshwater major carp, cirrhinus mrigala (hamilton) in relation to food and feeding habits.1. various developmental stages of taste bud have been observed in the fry, fingerling and adult of cirrhinus mrigala (hamilton). 2. the fries are zooplankton-feeders (carnivorous) and sight-feeders. therefore, functional taste buds are absent in the lip and bucco-pharyngeal epithelia. only formative stages of taste bud are present. some epithelial cells aggregate together to form this structure which has no function in gustation. moreover, the fires have low r. l. g. valle (0.71;..1.20) due to ...19751224765
[effect of two rearing conditions on growth and body composition in carp (cyprinus carpio l) (author's transl)].a comparative study was made of the growth and of the influence of seasonal changes on nitrogen and carbohydrate metabolism of carp kept in batches in natural ponds. 10 growth is active only in natural ponds. in batches where only industrial dried foods are used, no growth can be measured. 20 activity of ribonucleic and protein metabolism is correlated with seasonal variations of water temperature in both conditions.20031223266
[triploidy in hybrids of carp with other representatives of the cyprinidae family].as a result of remote hybridization of carp (cyprinus carpio l.) with the representatives of certain other subfamilies of cyprinidae a few viable f1 hybrids were obtained for the first time. these hybrids proved to be allotriploids. in the cross cyprinus carpio l. female x hemiculter eigenmanni (jord. et metz.) male the rate of survival of this year's brood was 0,5% of the fertilized eggs and 3n=128; whereas in the cross cyprinus carpio l. male x ctenopharyngodon idella (val.) female the rate of ...19751218729
[diploid radiation gynogenesis in carp. i. massive production of diploid gynogenetic progeny].mass lost of diploid gynogenetic carp offspring were obtained with the use of fish-farm method of reproduction. the average yield of gynogenetic diploids in usual experiments was 0.1% (from fertilized eggs). the cooling of unfertilized spawn (at the stage of metaphase ii) to 8--10 degrees c during 3.5--4.5 hours in 50% of cases permitted to increase by tens of times (in the most successful experiments up to 8%) the yield of gynogenetic diploids. the rate of survival of carps remained relatively ...19751218724
functional morpho-histology of the alimentary canal of an indian frewhwater major carp, labeo rohita (hamilton) during it's different life-history stages.1. the alimentary canal of the fry of l. rohita (hamilton) is of the carnivorous type in nature and disposition with a low relative length of the gut (r. l. g.). 2. end-buds are present. the buccopharyngeal region has a simple epithelial surface. mucous cells are small and few throughout the length of the alimentary canal. the presence of these structures in association with a zooplankton feeding habit indicate that the fries are primarily sightfeeders and carnivorous. 3. with increase in age an ...19751217739
[influence of some pesticides on the ontogenesis of carp]. 19751216612
[panfuran in aeromonosis (rubella) of carp]. 19751216501
[peptide hydrolase activity during embryonic carp development].it was established that in the carp the process of embryonic and early postembryonic development was accompanied by the increase in the activity of both the trypsin- and chemotrypsin-like peptide hydrolases. this increase is most evident in larvae with resorbing yolk. the total and protein nitrogen content suffers no changes during embryonic and early postembryonic development. the content of non-protein nitrogen increases from the stage of the middle of segmentation on attaining the maximum lev ...19751214997
water flow dynamics in the respiratory tract of the carp (cyprinus carpio l.).simultaneous measurements of water velocity in the buccal chamber, and buccal and opercular hydrostatic pressure of carp have revealed surprisingly high water velocities. the high flow velocities mean that, at times, the kinetic energy of flow makes a substantial contribution to the total fluid energy. this suggests that there may be unequal distribution of hydrostatic pressures within the buccal chamber. anatomical examinations showed that fluid channels in the buccal chamber and gill raker sie ...19751214116
the use of carp, cyprinus carpio (linnaeus, 1758), instead of rabbit as antibody donor in immunological research on fishes.antisera produced by carp were tested in the differentiation between two cyprinid fishes, bream and roach, and between the lipovitellins of two grey mullets, mugil cephalus and liza ramada. these antisera were more specific in the recognition of different fish species than those produced by rabbits. an antiserum against carp lipovitellin was produced with male carp as antibody donor.19751211668
[structural and immunochemical studies of the immunoglobulins of carp (cyprinus carpio l.) ii. analysis of the subunits].the 14 s immunoglobulin of the carp (cyprinus carpio l.) was split into subunits with 0,01 m dithioerythritol. these 5,7 s subunits have a molecular weight of 104 000 and a hexose and hexosamine content of 6.2%. it is likely that the subunits represent hl-half-molecules and not h2l2-monomeres. the values for the molecular of h- and l-chains were 77 000 and 24 000, respectively.19751210999
[the constancy of form recognition with different inversions of visual stimuli in fish (cyprinus carpio)]. 20031210739
[mechanisms of recognizing geometric figures and their elements--angles in fish (cyprinus carpio)].a study was made of the mechanisms of discrimination of geometrical figures and their angles by fish and of the influence of repeated presentation of stimuli on recognition time. the experiment was made by the conditioning method with the use of two variants of food-procuring technique: simultaneous and successive choice of stimuli. it was shown that discrimination of geomentrical figures is achieved at the fist stage by the mechanism of selection, and after long training, by the mechanism of ca ...20061210662
dynamic characteristics of the receptive field of l-cells in the carp retina. 19751210026
[intracellular on--off responses of carp retina amacrine cells adapted to the dark].dependence of the amplitude and latency of on and off responses of amacrine cells in the isolated dark-adapted carp retina upon the light spot intensity and diameter was studied by intracellular recordings. it is shown that the responses of amacrine cells to onset and offset of light consist of trasient depolarizations with oscillatory potentials and impulses. the latency of the on response decreases and the latency of the off response increases when the light spot intensity and diameter are inc ...19751207845
[electrical characteristics of the granular neurons of the carp olfactory bulb].using the intracellular recording technique the electrical activity of the carp olfactory bulb granular cells and secondary neurons was studied. early and late epsp and ipsp were the synaptic responses of granular cells both to the olfactory nerve and olfactory tract electrical stimulation. the comparison of responses of the granular cells and secondary neurons led to the conclusion that the granular cells are interneurons producing post-synaptic inhibition of the secondary neurons. it was suppo ...19971207841
multiple sclerosis-associated agent: transmission to animals and some properties of the agent.in confirmation and extension of observations by carp and his associates, brain tissue and sera from patients with multiple sclerosis (ms) were found to harbor an agent which induces a transitory depression in polymorphonuclear leukocytes (pmn) in mice as well as in rats, hamsters, and guinea pigs. all of eight md brains contained this agent at titers as high as 10(-9)/g of brain tissue. the agent was found in ms sera at titers up to 10(-3)/ml of serum, but its presence depended to some extent o ...19751205617
ultrastructural localization of synaptic input to the optic lobe of carp (carassius carassius). 19751204704
[effects of different toxic compounds (pb, cu, formol, nh4) on the carp: histologic changes in excretory and hematopoietic organs].here is a comparative study of the impairments observed on the histological structure of the excretory and hematopoietic organs of carps after acute intoxication (by formol and ammonia) and chronic intoxication (by copper sulfate and lead nitrate). although all these toxic substances induce the same type of impairments, their degree of seriousness vary. the most affected organs are the gills which present with congestive reactions and partial destruction of respiratory lamellae, and the spleen w ...20131204674
responses of the ultimobranchial body in eels (anguilla anguilla l.) maintained in sea water and experimentally matured, to injections of synthetic salmon calcitonin.the effect of a synthetic salmon calcitonin (sct) treatment on ultimobranchial body (ub) activity in eels (anguilla anguilla l.) maintained in seawater and submitted to experimental maturation, has been studied histologically. 2. the activity of the glands of a control group of eels maintained in sea water was taken as a reference. 3. the ub parenchyma showed a marked atrophy in the fish treated with sct alone and serum calcium decreased significantly in this group. 4. immature female silver eel ...19751201598
studies on the nutrition of marine flatfish. the thiamin requirement of turbot (scophthalmus maximus).1. seven groups of young turbot (scophthalmus maximus) were given diets containing graded levels of thiamin (0-19--50 mg/kg) for 16 weeks and their growth rate was measured during this period. 2. good growth was obtained on all these treatments except in the group given the lowest dietary thiamin level (0-19 mg/kg). these fish grew normally until the 12th week but thereafter their weight did not increase. 3. measurements of erythrocyte transketolase (sedoheptulose-7-phosphate: d-glyceraldehyde-3 ...20051201262
morpho-histology of the intestine in a freshwater major carp, cirrhinus mrigala (hamilton) during the different life-history stages in relation to food and feeding habits.the morpho-histology in relation to food and feeding habits of the intestine in the fry, fingerling and adult of cirrhinus mrigala (hamilton), a freshwater major carp, has been described. the intestine has been observed to be shortest in the fry (carnivorous) and longest in the adults (herbivorous) with intermediate values in the fingerlings (omnivorous). the mucosal folds of the intestine have also been observed to be adapted according to the nature of the dietary prevailing in the different li ...19751200390
studies on phagocytosis in fish. i. in vitro uptake and killing of living staphylococcus aureus by peripheral leucocytes of carp (cyprinus carpio).the clearance and uptake of staphylococcus aureus were studied in vitro in whole blood of immunized and non-immunized carp. bacterial numbers were determined by : (1) counting viable cells in agar plates; (2) counting radioactivity of 14c-labelled bacteria. similar results were obtained by these two methods. neither the bacterial growth nor the ingestion of bacteria into the cells seem to be influences by immunization. in both immunized and non-immunized groups the number of intracellular bacter ...19751193688
the histones of rainbow trout erythrocytes include an erythrocyte-specific histone.the erythrocyte histones of rainbow trout were compared with those of goose by polyacrylamide gel electrophoresis. a band analogous to goose erythrocyte-specific histone v, but not identical in relative mobility or quantity, was found to be a component of trout erythrocyte histone. a similar component was also found in carp erythrocyte histone, but it was absent from trout liver histone. to reveal this band clearly, it was advantageous to displace the histone iii monomer by oxidation. to verify ...19751192258
structure and innervation of extraocular muscles of carassius.the extraocular muscles of the carp carassius contain two types of muscle fibre. large white fibres have ribbon-shaped peripheral myofibrils and triads located at the z line. small red fibres, rich in mitochondria, have polygonal-shaped myofibrils and triads at the a-i junction. silver- and cholinesterase-stained preparations show that the large fibres are innervated by axons which spiral around them and exhibit intense cholinesterase activity over long distances. axons supplying small muscle fi ...19751184453
a dynamic model of the receptive field of l-cells in the carp retina. 19751182232
sensitivity of eels (anguilla anguilla) and carp (cyprinus carpio) to type c and e botulinum toxin.this study has shown for the first time that fish such as eels (anguilla anguilla l.) and carp (cyprinus carpio l.) are sensitive to type e botulinum toxin. with oral administration the mld for eels was 27.500 mouse ld50 per kg body weight, while for carp it was only 236 mouse ld50 per kg. carp were also found to be sensitive to a slight degree to type c botulinum toxin, the mld of the most virulent toxin of this type being 457,000 mouse ld50 per kg with oral administration. eels, however, showe ...19751179868
fish rhabdoviruses: comparative study of protein structure.proteins from four fish rhabdoviruses have been studied by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. the viruses were: trout viral hemorrhagic septicemia (vhs), infectious hematopoietic necrosis virus (ihn), spring viremia virus of carp (svc), and the pike fry rhabdovirus (pfr). for the two salmonid viruses (vhs-ihn), gel electrophoresis indicated the proteins, with molecular weights estimated to be 190,000, 80,000, 38,000, 25,000, and 19,000, respectively. the electrophoretic p ...19751171263
physico-chemical and serological characterization of five rhabdoviruses infecting fish.viruses isolated from fish with viral haemorrhagic septicaemia (vhs), infectious haematopoietic necrosis (ihn), spring viraemia of carp (svc), swim-bladder inflammation (sbi) and pike fry disease (pfd) have been grown to high titre in fathead minnow cells. while our preparations of the ihn, svc, sbi and pfd viruses showed typical rhabdovirus morphology with bullet-shaped particles and distinct surface projections, the vhs virus preparations had a less typical rhabdovirus morphology but were pleo ...19751170278
significant differences in the degradation of pro-leu-gly-nh2 by human serum and that of other species (38484).the acyclic c-terminal tripeptide of oxytocin, h-pro-leu-gly-nh-2, is not degraded upon incubation with human (male,female or pregnant female) plasma or serum for 1hr at 37 degrees. however, the sera of other species tested, including rat, chicken and carp, degrade this tripeptide 100%, 4% and 30%, respectively, in 1 hr, as determined by quantitative amino acid analysis of released products. among the species studied there seems to exist a correlation between the anatomic development of the pars ...19751168915
blocking and overshadowing in two species of fish.in experiment 1, a mixed blocking-overshadowing effect of color on an auditory discrimination was demonstrated in goldfish. in experiment 2 (with lines differing in color and angle), blocking of angle by color and of color by angle was demonstrated in goldfish, in experiment 3 (again with lines differing in color and angle), overshadowing of angle by color was demonstrated in carp, but the same animals (like goldfish in a previous study) failed to show greater intradimensional than extradimensio ...19751151288
[ultrastructure and functions of the cells of proximal and distal segments of the nephron of anadromous and freshwater fishes].in the carp kidney, the proximal tubule cells have no infoldings of the basilar plasmatic membrane. in the salmonidae nephrons the numerous parallel membranes of proximal tubule cell are formed by foldings of the basilar plasmatic membrane with numerous mitochondria in between. in o. nerka and s. malma experiments with mgcl2 injection showed that their kidneys secrete magnesium in contrast to the carp kidney. the numerous invaginations of proximal tubule cells membrane are suggested to be a morp ...19751145756
mechanical responses to linearly rising currents in the red and white muscles of the silver carp.the mechanical response of the red and white muscles of the silver carp was investigated using linearly stimulating currents. in the presence of tetrodotoxin, the peak tension increased when the rate of rise was reduced to about 0.3 ma/sec in the red muscle, and to about 3 ma/sec in the white muscle, provided that the maximum current applied always reached 3 ma. further reduction of the rate of rise decreased the tension, particularly in the white muscle. low concentrations of caffeine (0.5 mm) ...20051142568
anaerobic metabolism in the carp (carassius carassius l.). 19751139891
the unanswered question of high anaerobic capabilities of carp white muscle. 19751139461
[partial characterization of a hypothalamic gonadotropic hormone releasing factor in carp (cyprinus carpio l.) studied in vitro]. 19751132656
[ induction of pituitary gonadotropin discharge in the carp (cyprinus carpio l.) with the aid of cisclomiphene citrate]. 19751132655
[the ultrastructure of carp granulocytes]. 19751131027
comparative biochemistry of serum albumin. a serum albumin-like protein from carp, cyprinus carpio. 19751122738
slow piii component of the carp electroretinogram.the slow piii component of the electroretinogram (erg) was studied in the isolated, aspartate-treated carp retina. although the latter is richly populated with cones, slow piii appeared to reflect almost exclusively the activity of rods; e.g. the spectral sensitivity of the potential paralleled closely the rod pigment curve, its operating range (i.e. the v-log i curve) was limited to 3 log units above absolute threshold, and raising background intensities to photopic levels produced saturation o ...19751117278
temperature dependence of cleavage rates in carp, pike, and whitefish.the time taken by the first four cleavage divisions has been determined for the carp, pike, and whitefish (coregonus peled) at various temperatures; a quantity tau-0 was calculated, and plotted against temperature. from the curves plotted for all species investigated, it was found that in the salmon but not in the more thermophile species the rate of division within the spawning temperature range could be described by a constant q 10 which had a value exceeding 2 or 3. criteria for determing the ...19751114350
the central projections of the trigeminal, facial and anterior lateral line nerves in the carp (cyprinus carpio l.).the central projections of the trigeminal, facial and anterior lateral line nerves were studied in the carp (cyprinus carpio) by the nauta and fink-heimer silver techniques following rhizotomy. degenerating trigeminal fibers were found projecting on the nucleus of the descending trigeminal root and on the medial funicular nucleus. the former can be subdivided in five portions lying dorsal to the various cranial motor nuclei. the afferent facial fibers could be traced into the facial, glossophary ...19751112930
troponin and parvalbumin calcium binding regions predicted in myosin light chain and t4 lysozyme.a computer search of available protein sequences and structures suggests that bacteriophage t4 lysozyme contains one region and that rabbit myosin light chains contain three regions similar, and supposedly homologous, to the calcium binding region of carp muscle calcium binding parvalbumin.19751111094
proceedings: cross-reaction of rabbit anti-carp gonadotrophin globulin with gonadotrophic hormone of some teleost fish as tested by immunofluorescence. 19751094085
[action potentials of the heart atria and ventricles of rana temporaria frogs and cyprinus carpio carp].using microelectrode technique, studies have been made on electrophysiological indices (amplitude of ap, amplitude of the plateau, latent period of ap, duration of maximal depolarization, duration of repolarization at different levels) of cells of isolated atrium and ventricles of the carp during both the spontaneous activity and electrical stimulation. the obtained amplitude-temporal parameters were compared to those of the heart in the frog r. temporaria. it was found that the amplitude of ap, ...20061088024
[studies on the hard tissues of the pharyngeal teeth of carp]. 19761072931
Displaying items 7101 - 7200 of 7554