Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| [microbiological research methods of drinking water regulation in west germany from 1986. suitability of the specifications of din 38411, part 7, for the detection of sulfite-reducing, spore-forming anaerobes (clostridia)]. | the drinking-water regulations of the federal republic of germany, from 22.05.1986, contains in paragraph 1 the instructions: "drinking-water must be free of pathogens", and further in paragraph 11, "responsibilities of the employer or other owner of a water supplying facility", include that: "the official authority may direct, that the employer...of a water supplying facility has to extend or has to cause to extend the microbiological examinations in order to determine, that...sulfite-reducing, ... | 1988 | 2897749 |
| haemolytic patterns for presumptive identification of clostridium perfringens type c. | 1988 | 2897738 | |
| influence of clostridium perfringens and its toxin in germ-free chickens. | twenty-one of 56 germ-free chickens died after receiving an oral inoculation of a broth culture of clostridium perfringens. at necropsy there was detachment and disruption of the epithelial layer at the tips of villi and sloughed epithelial cells in the duodenum. these are characteristic lesions of necrotic enteritis. germ-free chickens receiving either purified alpha-toxin or the supernatant of broth cultures of c perfringens died, but no bird died after receiving supernatant of broth cultures ... | 1988 | 2897708 |
| evaluation of in vitro methods for testing ceftriaxone against anaerobic bacteria, including quality control guidelines. | tests with 100 anaerobic bacterial isolates demonstrated comparability between ceftriaxone mics obtained with the reference agar dilution procedure and those obtained with a broth microdilution susceptibility testing procedure. the aerobically incubated thioglycolate disk elution test was also evaluated. six 30-micrograms ceftriaxone disks in 5 ml of thioglycolate separated strains for which mics were less than or equal to 32 micrograms/ml from those for which mics were greater than or equal to ... | 1988 | 2896669 |
| inhibition of n-acetylneuraminate lyase by n-acetyl-4-oxo-d-neuraminic acid. | we show that the 4-oxo analogue of n-acetyl-d-neuraminic acid strongly inhibits n-acetylneuraminate lyase (neuac aldolase, ec 4.1.3.3) from clostridum perfringens (ki = 0.025 mm) and escherichia coli (ki = 0.15 mm). in each case the inhibition was competitive. n-acetyl-d-neuraminic acid; n-acetylneuraminate lyase; n-acetyl-d-neuraminic acid analog; 5-acetamido-3,5-dideoxy-beta-d-manno-non-2,4-diulosonic acid; 2-deoxy-2,3-didehydro-n-acetyl-4-oxo-neuraminic acid; competitive inhibitor. | 1988 | 2896604 |
| human platelet activation by bacterial phospholipase c: mechanism of inhibition by flurazepam. | we have shown earlier that phospholipase c (plc) from clostridium perfringens causes platelet activation possibly by inducing turnover of phosphoinositides and phosphorylation of a 47,000 dalton protein (p47). moreover, only 15 microm and 11 microm flurazepam inhibits plc-induced platelet aggregation and serotonin secretion by 50% respectively. this study was conducted to better understand the mechanism of platelet activation by plc and its inhibition by flurazepam. incubation of (14c)-arachidon ... | 1988 | 2896397 |
| studies on metabolic regulation and estimation of sialic acids by enzyme immobilization techniques. | 1988 | 2896358 | |
| molecular characterization of the resolvase gene, res, carried by a multicopy plasmid from clostridium perfringens: common evolutionary origin for prokaryotic site-specific recombinases. | clostridium perfringens strain cpn50 harbours a 10.2 kb plasmid known as pip404 which, in addition to a set of uv-inducible genes involved in bacteriocin production, carries res, a gene probably encoding a site-specific recombinase. the res protein is highly homologous to the resolvases of transposons from both gram-negative and gram-positive bacteria as well as enzymes involved in site-specific dna inversion. a likely role for the res protein would be to stabilize pip404 by reducing the number ... | 1987 | 2896291 |
| characterization of an outbreak of clostridium perfringens food poisoning by quantitative fecal culture and fecal enterotoxin measurement. | published criteria for implicating clostridium perfringens as the cause of food-poisoning outbreaks include finding a median fecal c. perfringens spore count of greater than 10(6)/g among specimens from ill persons. we investigated a food-poisoning outbreak with the epidemiologic characteristics of c. perfringens-related disease in a nursing home in which the median fecal spore count for ill patients (2.5 x 10(7)/g) was similar to that for well patients (4.0 x 10(6)/g), making the etiology of th ... | 1988 | 2895776 |
| effect of perfringolysin o on the lateral diffusion constant of membrane proteins of hepatocytes as revealed by fluorescence recovery after photobleaching. | perfringolysin o is a thiol-activated cytolytic exotoxin the primary receptor of which is the membrane cholesterol on the cell surface. the effect of perfringolysin o was tested in various hepatocyte preparations. (i) smears of fresh liver exposed to a mild h2o2 (1.0 mm) injury for 10 min at 37 degrees c, develop a 'peroxide-induced autofluorescence' (piaf) on the membrane proteins. piaf is suitable for measuring the average lateral diffusion constant (d) of the membrane proteins by means of flu ... | 1988 | 2895669 |
| clostridial cystitis in a nondiabetic man: case report. | 1988 | 2895555 | |
| the passive protection of lambs against clostridium perfringens type d with semi-purified hyperimmune serum. | weaned lambs, having a detectable level of maternal antibodies (1-2 units/ml) against c. perfringens type d, showed protective antitoxin levels lasting for 29 days after receiving a single parenteral dose of 200 units/kg hyperimmune serum. lambs, having no maternal antibodies (less than 0,07 units/ml) to c. perfringens type d but receiving the same dose of hyperimmune serum, maintained protective antibody levels for only 21 days. three weeks after the titres fell below the minimum protective lev ... | 1988 | 2895443 |
| hematogenously acquired infection of a total knee arthroplasty by clostridium perfringens. | a 64-year-old man with total knee arthroplasty for tricompartmental osteoarthritis had a postoperative course complicated by an attack of acute cholecystitis and was treated with cholecystectomy. the tka was hematogenously seeded with clostridium perfringens, which necessitated emergency removal of the implants, debridement, and ultimately arthrodesis. | 1988 | 2894912 |
| clostridial septic arthritis: case report and review of the literature. | we describe a patient who had septic arthritis caused by clostridium perfringens. clostridial organisms are very uncommon causes of septic arthritis. only 13 cases have been reported previously. the diagnosis should be suspected in patients with a history of penetrating joint trauma and in immunocompromised patients. successful treatment has usually consisted of surgical synovectomy in combination with high-dose intravenous penicillin therapy. multiple aspirations of affected joints as a definit ... | 1988 | 2894833 |
| experimentally induced necrotic enteritis in chickens. | a 1.3 to 10% incidence of necrotic enteritis was experimentally produced in broiler-type chickens in three of five trials. the incidence range observed was considerably narrower and lower than the 5.6-37.3% range reported in the literature. clostridium perfringens was inconsistently isolated from the liver and intestine of dosed chickens. | 1987 | 2894826 |
| experimental induction of abdominal tympany, abomasitis, and abomasal ulceration by intraruminal inoculation of clostridium perfringens type a in neonatal calves. | the etiologic role of clostridum perfringens type a in the acute abdominal syndrome characterized by abomasal and rumen tympany, abomasitis, and abomasal ulceration was investigated in neonatal calves. eight calves, 4 to 12 days old, were inoculated intraruminally with toxigenic c perfringens type a. before and after c perfringens inoculation, blood samples were collected from all calves for blood gas and serum biochemical analysis and for determination of serum copper concentration; ruminal flu ... | 1988 | 2894790 |
| development of a new shuttle plasmid system for escherichia coli and clostridium perfringens. | we constructed a 7.9-kilobase-pair recombinant shuttle plasmid, designated phr106, by combining desired segments of three plasmids: an escherichia coli plasmid (psl100) which provides a multiple cloning site, a clostridium perfringens plasmid (pju122) which provides a clostridial origin of replication, and an e. coli plasmid (pjir62) which provides an e. coli origin of replication, an ampicillin resistance gene, and a chloramphenicol resistance gene of clostridial origin. the shuttle plasmid tra ... | 1988 | 2894200 |
| transformation of clostridium perfringens l forms with shuttle plasmid dna. | l-form (l-phase) cultures of clostridium perfringens were tested for their transformability with plasmid dna. three l-form strains were transformable, but one, strain l-13, was superior to the others. this strain was easily and reproducibly transformed with previously described shuttle vectors which were derived from either c. perfringens or escherichia coli. strain l-13 was transformable by a variety of methods, and a new micromethod worked well under both aerobic and anaerobic conditions. the ... | 1988 | 2894199 |
| diffusion of metronidazole through the dentinal tubules of extracted teeth. | passing of metronidazole from the root canal of extracted gangrenous teeth through the dentinal tubules was proved by agar diffusion and minimum inhibitory concentration assay. the findings explain the excellent clinical experience with metronidazole in root treatment. | 1987 | 2894107 |
| presence of clostridium perfringens enterotoxin in intestinal samples from farm animals with diarrhoea of unknown origin. | 1987 | 2894102 | |
| botulinum type d toxin and clostridium perfringens enterotoxin in a bull calf. | 1987 | 2894086 | |
| [isolation of clostridium perfringens from cooked meat foods]. | 1987 | 2894047 | |
| nonimmune binding of ig to clostridium perfringens. preferential binding of igm and aggregated igg. | in an attempt to find a bacterial igm receptor, a large number of bacterial strains of different species were screened for the ability to bind human igm. certain strains of the anaerobic bacterium clostridium perfringens were found to bind a major fraction of polyclonal igm. one bacterial strain showed a particularly high binding capacity and was studied in more detail. this strain is also able to bind a minor fraction of polyclonal iga and igg. inhibition experiments indicate that the different ... | 1988 | 2893826 |
| intracellular processing of cytidylyltransferase in krebs ii cells during stimulation of phosphatidylcholine synthesis. evidence that a plasma membrane modification promotes enzyme translocation specifically to the endoplasmic reticulum. | after a 3-h incubation of krebs ii ascitic cells in the presence of phospholipase c from clostridium welchii under nonlytic conditions, the incorporation of [3h] choline into phosphatidylcholine was increased 1.7-fold as compared to untreated cells. the total amounts of phosphatidylcholine, phosphatidylethanolamine, and sphingomyelin were unchanged up to 3 h of incubation. the limiting step in phosphatidylcholine biosynthesis was the formation of cdp-choline catalyzed by ctp:choline-phosphate cy ... | 1988 | 2893798 |
| enumeration of clostridium perfringens spores in human feces: comparison of four culture media. | enumeration of clostridium perfringens spores was compared using 4 culture media. duplicate 1 g portions of 35 stools (25 from c. perfringens food poisoning outbreaks and 10 from normal stools) were heat treated 20 min at 75 degrees c and tested on tryptose-sulfite-cycloserine (tsc) agar, trypticase-soy-blood (tsb) agar, lactose-sulfite (ls) medium, and iron milk (im) medium. dilutions were plated directly onto tsb and tsc, and a 3-tube most probable number determination was made with each speci ... | 1987 | 2893783 |
| alkyl-linked diquinolines are monofunctional at-selective dna-intercalating agents. | the binding of a homologous series of alkyl-linked 4-aminodiquinolines to circular and linear dnas was studied using viscometric titrations and equilibrium dialysis. the compounds are monofunctional intercalators with the capacity for intercalative binding reaching a peak for the heptane homologue. they show marked at-base pair selectivity, which suggests that the non-intercalated quinoline ring may lie in the dna minor groove. affinities for calf thymus dna increase as the alkyl chain is length ... | 1988 | 2893746 |
| a large clostridium perfringens foodborne outbreak with an unusual attack rate pattern. | on november 7, 1985, a clostridium perfringens gastroenteritis outbreak occurred in approximately 44% of the 1,362 employees at a connecticut factory. although the same foods were served to all three shifts at an employee banquet on november 6, the attack rate was almost twice as high for those who ate on the day shift (attack rate = 50%) than for those on the evening shift (attack rate = 20%) or night shift (attack rate = 29%). among employees of the day shift, attack rates were highest for tho ... | 1988 | 2893540 |
| [specificity against group a, c, and g streptococci of the reverse camp test for identifying clostridium perfringens]. | we studied "reverse camp" test specificity for the identification of clostridium perfringens, and the probable existence of cross-reactions with groups a, c and g streptococci. ninety eight clostridium strains were tested with ten s. agalactiae, ten s. pyogenes, two group c streptococci and one group g. all the strains of s. agalactiae yielded a positive "reverse camp" reaction when it was performed with c. perfringens strains. cross-reactions were not detected when c. perfringens were tested wi ... | 1985 | 2893427 |
| adp-ribosylation of skeletal muscle and non-muscle actin by clostridium perfringens iota toxin. | the enzymatically active component ia of clostridium perfringens iota toxin adp-ribosylated actin in human platelet cytosol and purified platelet beta/gamma-actin, in a similar way to that been reported for component i of botulinum c2 toxin. adp-ribosylation of cytosolic and purified actin by either toxin was inhibited by 0.1 mm phalloidin indicating that monomeric g-actin but not polymerized f-actin was the toxin substrate. perfringens iota toxin and botulinum c2 toxin were not additive in adp- ... | 1988 | 2892681 |
| growth curves of clostridium perfringens in schaedler and brain heart infusion broths. | four clostridium perfringens strains recovered from clinical specimens, four strains obtained from faecal specimens of healthy adults, and control strain atcc 13124 were inoculated into schaedler and brain heart infusion broths, respectively, and incubated anaerobically at 35 degrees c for 24 h. the exponential growth phase usually lasted from 2 to 6 h following inoculation; the generation times averaged 23 min at 2 to 4 h and 27 min between 4 and 6 h of incubation. the early stationary growth p ... | 1987 | 2892327 |
| [anaerobic spore formers in commercial spices and ingredients for infant food]. | anaerobic spore-formers can cause food intoxication. clostridium (cl.) perfringens type-a strains are mainly involved. in addition to the original contamination of food, e.g., meat or milk, ingredients such as spices or meat extracts are the main source and low numbers of clostridia are present (i.e., up to 10(2) cells/g). at present, information about the quantitative ecology of clostridia is incomplete; therefore 115 commercial food products were investigated (spices: n = 70, meat extracts: n ... | 1987 | 2892325 |
| response of ligated rabbit ileal loop to clostridium perfringens type c strains and their toxic filtrates. | the vegetative cells and toxic filtrates of clostridium perfringens type c strains were injected into ligated rabbit ileal loops and the responses were observed. out of 12 strains examined, 2 strains showed positive reaction in this test, when the vegetative cells were injected. one of these 2 strains was an enterotoxigenic and beta-toxigenic and the other was beta- and delta-toxigenic but not enterotoxigenic. culture filtrates containing beta or delta toxin also showed fluid accumulation in the ... | 1987 | 2892115 |
| infectious diarrhea due to clostridium perfringens. | 1988 | 2891779 | |
| lethal effects and cardiovascular effects of purified alpha- and theta-toxins from clostridium perfringens. | shock, a common and frequently fatal manifestation of gas gangrene caused by clostridium perfringens, is probably mediated by extracellular toxins. previous studies implicating alpha-toxin as the major lethal factor were frequently done with preparations contaminated with a second lethal factor, theta-toxin. we purified alpha- and theta-toxins from c. perfringens and demonstrated that both were lethal to mice. we investigated the effects of these purified toxins on cardiovascular function in int ... | 1988 | 2891775 |
| clostridium perfringens iota toxin adp-ribosylates skeletal muscle actin in arg-177. | clostridium perfringens iota toxin adp-ribosylates actin. substrates of c. perfringens toxin are both non-muscle beta/gamma-actin and skeletal muscle actin. this finding suggests that c. perfringens iota adp-ribosylates the same amino acid in skeletal muscle and non-muscle actin as does c. botulinum c2 toxin in non-muscle actin. protein chemical analysis involving thermolysin cleavage on [32p]adp-ribosylated actin or tryptic digestion followed by a secondary thermolysin cleavage of the radiolabe ... | 1987 | 2891567 |
| changes in the hydrophobic characteristics of clostridium perfringens spores and spore coats by heat. | the hydrophobic characteristics of clostridium perfringens nctc 8679 spores were demonstrated by adherence to toluene in a toluene-aqueous partition system. spores and spore coat preparations were hydrophobic. vegetative cells and spores extracted with a dithiothreitol-sodium dodecyl sulfate treatment known to remove spore coats were not hydrophobic. a heat activation treatment (75 degrees c for 20 min) which promotes more rapid spore germination increased the hydrophobicity of intact spores and ... | 1987 | 2891427 |
| [use of the peroxidase method of immunoenzyme analysis for detecting clostridium perfringens cells]. | the possibility of using the peroxidase method of the enzyme immunoassay for the detection of c. perfringens cells in the culture fluid of strains, as well as in affected tissues in cases of anaerobic gas gangrene infection, has been shown. this method is highly specific, convenient and takes but 1.5 hrs. | 1987 | 2891231 |
| influence of a wheat diet on mortality of broiler chickens associated with necrotic enteritis. | in an experiment to determine methods of incorporating soft winter wheat into broiler diets, a significant increase in mortality was observed in broilers fed wheat in crumbled diets. this increase in mortality was associated with necrotic enteritis with clostridium perfringens indicated as the causative pathogen and complicated by a coccidiosis outbreak. when yellow corn was used in the diet, mortality was 2.9%. use of all wheat, ground with a hammer mill, increased mortality to 28.9%. however, ... | 1987 | 2891128 |
| enterotoxigenicity of heat-resistant and heat-sensitive strains of clostridium perfringens. | 1987 | 2890785 | |
| isolation and function of a clostridium perfringens enterotoxin fragment. | a fragment was obtained by treating clostridium perfringens enterotoxin with 2-nitro-5-thiocyanobenzoic acid, a reagent which specifically cleaves the amino-terminal peptide bond of cysteine residues. the fragment (molecular weight, 15,000) was purified by high-performance liquid chromatography. the fragment had no cytotoxic effect on vero cells but competitively inhibited enterotoxin-induced 51cr release. binding of 125i-labeled fragment to vero cells was comparable to that of enterotoxin. more ... | 1987 | 2890582 |
| the stereoselectivity of the clostridium perfringens phospholipase c: hydrolysis of thiophosphate analogs of phosphatidylcholine. | thiophosphate containing analogs of phosphatidylcholine have been synthesized with varying degrees of structural complexity. these analogs have been used in a continuous spectrophotometric assay for phospholipase c from clostridium perfringens in order to examine the requirement for substrate ester functionalities and the stereoselectivity of the enzyme. the substrate analogs with ester groups in the nonpolar portion of the molecule were acceptable substrates for phospholipase c, while those ana ... | 1987 | 2890329 |
| enterotoxaemia in water buffaloes caused by clostridium perfringens type a. | 1987 | 2890229 | |
| clostridium perfringens meningitis in an infant: case report and literature review. | 1987 | 2890135 | |
| [effects of gall powder on the spore-forming and enterotoxin-producing abilities of clostridium perfringens]. | 1987 | 2889836 | |
| synthesis of a thiophosphate analog of dioctanoylphosphatidylcholine: a phospholipase c substrate. | dioctanoylthiophosphatidylcholine, a racemic thiophosphate analog of l-alpha-dioctanoylphosphatidylcholine, has been synthesized and isolated by flash chromatography. in contrast with the didecanoylthiophosphatidylcholine synthesized previously, the analog is easily dispersed on sonication in aqueous media and is rapidly hydrolyzed to produce a free thiol group in the presence of the extracellular phospholipase c from either bacillus cereus or clostridium perfringens. when 5,5'-dithiobis (2-nitr ... | 1987 | 2889786 |
| modulation of granulocyte functions by bacterial exotoxin and endotoxins. | the modulation of granulocyte functions by bacterial exotoxins (streptolysin o, alveolysin, theta toxin) and endotoxins from salmonella and lipid a is described here. incubation of polymorphonuclear granulocytes with thiol-activated toxins resulted in an increased leukotriene generation. toxin-pretreated pmns revealed an increased omega oxidation of ltb4, which may explain why toxin-stimulated cells release more ltc4 than ltb4. furthermore, toxin-pretreated pmns showed a decreased leukotriene ge ... | 1987 | 2889665 |
| [hygienic evaluation of the methods for utilizing wastes created in producing printed-circuit boards]. | 1987 | 2889645 | |
| effects of sulphasalazine on faecal flora in patients with rheumatoid arthritis: a comparison with penicillamine. | twenty-six out-patients with active rheumatoid arthritis (ra) were randomly allocated to treatment with sulphasalazine (sasp) or d-penicillamine (dpa). faecal samples were collected from all patients at 4-weekly intervals and examined for changes in faecal flora during treatment. both treatment groups showed substantial clinical improvement. in the sasp-treated group this was accompanied by significant falls in counts of cl. perfringens and e. coli. no such changes were seen in the dpa-treated g ... | 1987 | 2889501 |
| comparison of faecal florae in patients with rheumatoid arthritis and controls. | the faecal flora of 25 out-patients with active rheumatoid arthritis (ra) was compared with that of 25 age- and sex-matched controls. a comprehensive survey revealed a significantly higher carriage rate of clostridium perfringens in the ra population (88%) than controls (48%) (p less than 0.01). coliform counts also tended to be higher, but there were no other significant differences between patients and controls. when the study was enlarged to include a further 113 ra patients with variable dis ... | 1987 | 2889500 |
| [in vitro activity of mezlocillin on bacterial adhesion, phagocytosis and haemoagglutination of several anaerobic germs]. | 1987 | 2889247 | |
| [microbial inhibitors of phospholipase c]. | inhibitors of the clostridium perfringens phospholipase c were prepared from the filtrates of the culture liquids of streptomyces saracetidus and streptomyces species using soluble and cross-linked polyelectrolytes. the technological scheme of isolation involves ultrafiltration. the inhibitors produced by the two strains had different chemical nature. the preparation obtained from str. saraceticus was proved to be a complex of inhibitors that were separated by gel-chromatography into a major pol ... | 1987 | 2889205 |
| role of the essential thiol group in the thiol-activated cytolysin from clostridium perfringens. | a hemolysin, 0-toxin, produced by clostridium perfringens has one cysteinyl residue in the free thiol form which is essential for its hemolytic activity. the cysteinyl residue was shown to be located at a position about 5 kda from the c terminus of the molecule by the method of cysteine-specific chemical cleavage. modification of the residue with a thiol-blocking agent, 5,5'-dithiobis(2-nitrobenzoic acid), reduced the binding affinity of the toxin to sheep erythrocytes to 1/100 that of intact to ... | 1987 | 2888650 |
| t activation haemolysis and death after blood transfusion. | a 30 week gestation infant developed necrotising enterocolitis associated with clostridium perfringens septicaemia at 3 weeks of age. he responded to treatment with intravenous fluids, antibiotics, and blood, but his blood haemolysed. because of anaemia further blood was given, but, within minutes he died. examination of his red cells showed an increase in t activation. | 1987 | 2888436 |
| effect of infection with eimeria tenella upon the cecal bacterial population in monoflora chickens. | monoflora chickens were established at 2 days of age by an oral inoculation with one of six species of bacteria (lactobacillus acidophilus, bifidobacterium thermophilum, bacteroides vulgatus, clostridium perfringens, escherichia coli, or streptococcus faecalis) and were infected with eimeria tenella (5 x 10(4) oocysts per bird) 2 days later. there were two experimental groups for each bacterium: birds infected with bacteria alone and birds infected with a combination of bacteria and e. tenella. ... | 1987 | 2888103 |
| enteritis necroticans among khmer children at an evacuation site in thailand. | a severe illness characterised by bloody diarrhoea and intestinal dysfunction was recognised at an evacuation site on the thai-kampuchean border. from june, 1985, to july, 1986, the illness occurred in 62 khmer children aged 10 months to 10 years (mean 4 years); it was characterised by bloody diarrhoea (94%), fever (90%), and abdominal pain (78%). the overall mortality rate was 58%. among 16 children who died and underwent necropsy, small-intestinal necrosis of varying severity was found; in 5 o ... | 1987 | 2887787 |
| clostridium perfringens as the cause of death of a captive atlantic bottlenosed dolphin (tursiops truncatus). | a previously healthy captive female bottlenosed dolphin (tursiops truncatus) died suddenly. at necropsy, clostridium perfringens was isolated from dorsal muscle, blood, left heart ventricle, thoracic fluid, and abdominal fluid. an identical strain was recovered from pool water. a male dolphin in the same pool had inflicted several "rake" marks on the dorsal surface of the female. water-borne bacteria probably entered these lesions which served as the focus for anaerobe penetration and spread. | 1987 | 2887668 |
| a scanning electron microscope study of the effect of an enterotoxin from clostridium perfringens 8-6 on mice of different ages. | intestinal damage to mice caused by an enterotoxin from a coatless spore mutant of clostridium perfringens type a (8-6) was examined by scanning electron microscopy. two distinct types of damage were observed, both of which could be correlated with animal age. damage appeared to occur in a specific sequence similar to that found in previous studies in rabbits. we conclude that the type of ileal tissue damage reflects the mode of toxin incorporation from the gut, which is a function of animal age ... | 1986 | 2887627 |
| bilateral abscessed orchiepididymitis associated with sepsis caused by veillonella parvula and clostridium perfringens: case report and review of the literature. | veillonella species is a gram-negative coccus which is part of the anaerobic normal flora in the oral cavity, small intestine, upper respiratory tract, vagina, and urinary tract. the role that this organism plays in infection is not well known, and it is generally associated with other bacteria. we present a case of bilateral abscessed orchiepididymitis associated with septicemia due to veillonella parvula and, later, to clostridium perfringens, with the development of severe renal insufficiency ... | 1987 | 2887584 |
| proficiency testing of water microbiology laboratories in the netherlands. | in a 3-year period, four series of simulated water samples containing selected test strains were distributed to more than 50 laboratories in the netherlands for bacteriological testing. participating laboratories examined the samples by enrichment or membrane filtration methods, or both, for total coliform organisms, thermotolerant coliform organisms, faecal streptococci and standard plate counts (37 degrees and 22 degrees c) according to dutch standard methods. the results were quantitatively s ... | 1987 | 2887540 |
| computed tomography as the method of choice in the diagnosis of emphysematous cholecystitis. | computed tomographic (ct) findings in 4 cases of acute emphysematous cholecystitis are described. the use of ct in the diagnosis of this condition is discussed in relation to the use of sonography and plain radiography. | 1987 | 2887479 |
| raw beef, pork and chicken in japan contaminated with salmonella sp., campylobacter sp., yersinia enterocolitica, and clostridium perfringens--a comparative study. | one hundred and twenty samples each of raw ground beef, pork and chicken from ten local grocery stores in shimane prefecture, japan, were examined for the presence of salmonella sp. (sal), campylobacter jejuni (cj), campylobacter coli (cc), yersinia enterocolitica (ye), and clostridium perfringens (cp) from april 1984 to march 1985. a total of 205 isolates of sal (112 strains), cj (64 strains), cc (one strain), ye (7 strains) and cp (21 strains) were recovered from 17 beef (14.2%), 31 pork (25.8 ... | 1987 | 2887078 |
| clostridium perfringens in pelleted feed. | 1987 | 2887055 | |
| evaluation of some commercial media for the cultivation and enumeration of clostridium perfringens from the chick intestine. | four commercial media marketed for the detection and enumeration of clostridium perfringens were found to adequately support the growth of a pure culture (atcc 13124) of this species but enumeration of colony-forming units was difficult on clostrisel and lecithin-lactose-agar base (lla) media. colonies of c. perfringens on lla had typical opalescent zones, a distinctive feature that can aid in presumptive identification. colonies on sulfite-polymyxin-sulfadiazone and tryptose-sulfite-neomycin (t ... | 1987 | 2886986 |
| [pulpy kidney in the lamb]. | 1987 | 2886662 | |
| plasmid analysis as a means of strain differentiation in clostridium perfringens. | a total of 114 clostridium perfringens isolates were serotyped and examined for plasmids. fifty-two strains were from hospitalized patients with diarrhea or from hospital environments, and 62 epidemiologically unrelated isolates were obtained from food poisoning outbreaks. all strains were screened for bacteriocin production against a common indicator strain of c. perfringens. in the one significant hospital outbreak of c. perfringens diarrhea, three to five plasmid types were found in strains o ... | 1987 | 2886514 |
| a new endo-beta-galactosidase releasing gal alpha 1----3gal from carbohydrate moieties of glycoproteins and from a glycolipid. | culture fluid of clostridium perfringens contained an endoglycosidase releasing a galactosyl disaccharide from glycans derived from glycoproteins of teratocarcinoma ott6050. the endoglycosidase was purified by ammonium sulfate precipitation, sephadex g-200 column chromatography, and deae-sephadex a-25 column chromatography. the structure of the disaccharide product was determined to be gal alpha 1----3gal. the enzyme also released the disaccharide from bovine thyroglobulin glycopeptide and from ... | 1987 | 2886499 |
| isolation of clostridium perfringens from neonatal calves with ruminal and abomasal tympany, abomasitis, and abomasal ulceration. | eight neonatal calves (2 to 21 days old) with suspected abomasal displacement or intestinal obstruction after acute onset of abdominal tympany, colic, depression, or death were referred to kansas state university for clinical examination or for necropsy. results of routine hematologic and serum chemical analyses did not reveal consistent changes. necropsy revealed abomasal distention, with various degrees of abomasitis, hemorrhage, and ulceration, but did not reveal evidence of displaced abomasu ... | 1987 | 2886483 |
| toxigenic characteristics of clostridium perfringens type c in enterotoxemia of domestic animals. | eleven clostridium perfringens type c strains isolated from fatal cases of hemorrhagic enterotoxemia of canadian calves, a piglet, and a foal were studied for the production of soluble antigens. all the isolates from calves and a foal failed to produce delta toxin, but were capable of producing large amounts of lethal beta toxin. a strain isolated from a piglet produced delta, but very little beta toxin. other differences were relatively minor. the results indicated that young domestic animals m ... | 1987 | 2886206 |
| injection site reactions and antibody responses in sheep and goats after the use of multivalent clostridial vaccines. | uncertainty concerning the use, efficacy and possible adverse effects of clostridial vaccination in goats prompted a study of the injection site reactions and antibody responses in 40 goats and 40 sheep. the vaccines used were covexin 8, heptavac and tasvax 8. in all the animals swellings averaging 2.5 cm in diameter were present at the injection site seven days after vaccination and were still apparent 28 days after vaccination. the injection site reactions could not be attributed to faulty vac ... | 1987 | 2885963 |
| some bacterial diseases of spotted deer (axis axis). | 1987 | 2885962 | |
| [clostridium perfringens enterotoxemia. fatal form in a 5-year-old child]. | 1987 | 2885822 | |
| effects of alpha and theta toxins from clostridium perfringens on human polymorphonuclear leukocytes. | two toxins, alpha (phospholipase c) and theta (oxygen-labile hemolysin), were purified from clostridium perfringens type a and assayed for toxic effects on human polymorphonuclear leukocytes (pmnls). crude preparations containing both toxins totally inhibited chemotaxis and chemiluminescence responses of pmnls and reduced pmnl viability. purified alpha toxin did not alter pmnl viability, chemotactic responsiveness, or morphology but did enhance opsonized zymosan-induced pmnl chemiluminescence ov ... | 1987 | 2885383 |
| development and preliminary evaluation of a slide latex agglutination assay for detection of clostridium perfringens type a enterotoxin. | a slide latex agglutination (sla) assay was developed for rapid screening for clostridium perfringens type a enterotoxin (cpe). sla specifically detected cpe added to buffer or normal feces (sensitivity limit of 1 microgram cpe/g feces). using clinical fecal samples from c. perfringens food poisoning cases, a strong correlation was shown between sla results and results from other cpe assays and between sla results and illness status. | 1987 | 2885377 |
| evaluation of anaerobic microdilution mic results with the prompt inoculation system. | the prompt inoculation system (3m co.) was compared with the overnight suspension inoculation procedure used with a broth microdilution anaerobic commercial system (micro-media systems) for differences in mic. mic results from both suspension methods using six national committee for clinical laboratory standards-recommended quality control organisms were identical in 18 instances (75%) and within +/- 1 log2 dilution in 96% of the comparisons. results with 45 anaerobic clinical isolates also comp ... | 1987 | 2885341 |
| synthesis of p-nitrophenyl 5-acetamido-3,5-dideoxy-alpha-d-glycero-d-galacto-2-nonulopyranosid onic acid, a chromogenic substrate for sialidases. | 1987 | 2885090 | |
| isolation of clostridia, salmonellae and coccidia from wild pigeons in japan. | 1986 | 2885063 | |
| purification and kinetic properties of sialidase from clostridium perfringens. | clostridium perfringens sialidase was isolated from a culture medium of bacterial cells by ammonium sulfate precipitation (42-85%), followed by purification through sephadex g-75 gel chromatography, deae a-50 anion exchange chromatography, fplc medium pressure anion exchange chromatography and finally fplc medium pressure isochromatofocussing. from 9 l culture medium 1.17 mg sialidase was isolated with a specific activity of 295 u/mg. the enzyme appeared to be homogeneous by analytical polyacryl ... | 1987 | 2885013 |
| severe clostridium infection following perforation of the uterus in a patient with an ectopic pregnancy. | twenty-eight hours after co2 insufflation and curettage in a 26 year old, haemolysis, jaundice and anuria developed. as an ectopic pregnancy was also suspected, a diagnostic laparoscopy was done and was followed by a hysterectomy and left salpingo-oophorectomy before clostridium perfringens was grown on culture. the survival of the patient despite clostridial infection is probably due to immediate surgical treatment and intensive post-operative care. | 1987 | 2884930 |
| [gas gangrene]. | 1987 | 2884785 | |
| the inactivation of clostridium perfringens epsilon toxin by treatment with tetranitromethane and n-acetylimidazole. | when one residue of tyrosine per molecule of epsilon toxin was nitrated by tetranitromethane, the modified toxin lost more than 90% of its original activity. the toxin also was inactivated by treatment with n-acetylimidazole. the lethality was restored when the inactive n-acetylimidazole-treated toxin was treated with hydroxylamine. the inactive n-acetylimidazole-treated toxin was not nitrated by tetranitromethane under conditions where one residue of tyr per molecule of the untreated toxin is n ... | 1987 | 2884756 |
| detection of clostridium perfringens epsilon toxin by elisa. | an enzyme-linked immunosorbent assay (elisa) has been developed as an alternative to neutralisation tests in mice to detect clostridium perfringens type d epsilon toxin in the intestinal contents of animals which have died from suspected enterotoxaemia. the test was sensitive and quantitative and gave excellent agreement with the mouse protection test. | 1987 | 2884704 |
| purification of the alpha toxin of clostridium perfringens type a by ultrafiltration and gel chromatography. | clostridium perfringens type a toxin produced in jayko & lichstein medium was subjected to various concentration and purification procedures. the results obtained with 3 different ultrafiltration membranes followed by gel filtration showed that by using millipore psed ohv10 and amicon xm-100 filter membranes in combination, a three-hundred-and-fivefold purification could be achieved as against a twelvefold increase obtained with ammonium sulphate/acetone precipitation. the lecitovitelin test was ... | 1987 | 2884611 |
| a hypothesis concerning clostridium perfringens type a enterotoxin (cpe) and sudden infant death syndrome (sids). | this study identifies the presence of clostridium perfringens type a enterotoxin (cpe) in some gastrointestinal and serum samples from babies who had died of the sudden infant death syndrome (sids) and other causes. on occasion antibodies to this toxin were identified in sera. cpe is parasympathomimetic in its action. in the adult food poisoning model it is produced when the organism sporulates in vivo. this leads to speculation as to whether this toxin may play an ante-mortem role in the dying ... | 1987 | 2884556 |
| studies on nitrate reductase of clostridium perfringens. iv. identification of metals, molybdenum cofactor, and iron-sulfur cluster. | nitrate reductase of clostridium perfringens was purified by an improved method using immuno-affinity chromatography. the purified preparation contained mo, fe, and acid-labile sulfide; the mo content was 1 mol per mol and the fe 3.7 mol per mol of the enzyme. the inactive enzyme obtained from cells grown in the presence of tungstate did not hold mo but contained 1 mol of w. the content of fe was not increased. the presence of molybdenum cofactor in the nitrate reductase was indicated by the for ... | 1987 | 2884214 |
| [clostridium perfringens septicotoxemia]. | 1985 | 2883709 | |
| massive hemolysis in clostridium perfringens infections. | over a 14-month period at the cleveland clinic foundation, 424 strains of clostridium were isolated; of these, 52 strains were clostridium perfringens isolated from 41 patients. eight strains of c. perfringens were isolated from the blood of six patients; five of these patients had neoplastic disease and three developed massive intravascular hemolysis with rapidly developing shock and death. clinical details are given on three patients with fatal clostridium perfringens sepsis, and the nature of ... | 1987 | 2883342 |
| clostridium perfringens brain infection following a penetration wound of the orbit. | 1987 | 2883262 | |
| antagonistic effect exerted by three strictly anaerobic strains against various strains of clostridium perfringens in gnotobiotic rodent intestines. | our purpose was to study bacterial antagonism between a limited number of strictly anaerobic strains and clostridium perfringens in the intestinal tract of gnotobiotic rodents. gnotobiotic mice harboring a bacteroides thetaiotaomicron, a fusobacterium necrogenes, and a clostridium sp. strain were protected against pathogenic b, c, and d c. perfringens serotypes. a drastic antagonistic effect of this three-strain association was also observed against a nonpathogenic c. perfringens serotype a (cpa ... | 1987 | 2882830 |
| clostridium pyoarthrosis following arthroscopy. | 1987 | 2882761 | |
| chemical and biological properties of acetyl derivatives of the hydroxylamino reduction products of metronidazole and dimetridazole. | metronidazole and related 5-nitroimidazoles undergo reduction of their nitro group apparently to produce such reactive species as 5-hydroxylaminoimidazoles. to define the role of these species we have sought ways to prepare them by the catalytic reduction of metronidazole, dimetridazole and flunidazole. although their respective 5-hydroxylaminoimidazoles were too unstable to be isolated directly, their o,n-diacetyl derivatives were isolable. of these, the diacetyl derivative of the hydroxylamine ... | 1987 | 2882756 |
| comparison of single and combination antimicrobial agents for prevention of experimental gas gangrene caused by clostridium perfringens. | the treatment of experimental gas gangrene caused by clostridium perfringens was investigated by using combinations of antimicrobial agents. this study demonstrated that rifampin, penicillin, metronidazole, and clindamycin were all bactericidal against standard inocula (10(5) to 10(6) cfu). these antimicrobial agents were then administered to mice beginning 30 min after intramuscular injection of 10(9) cfu of c. perfringens type a. the highest doses used produced levels of drug in blood which ex ... | 1987 | 2882732 |
| effect of antibiotics on toxin production and viability of clostridium perfringens. | we have recently reported (d.l. stevens, k.a. maier, b.m. laine, and j.e. mitten, j. infect. dis. 155:220-228, 1987) that clindamycin, rifampin, and tetracycline were more efficacious than penicillin in the treatment of fulminant gas gangrene in mice caused by clostridium perfringens. we hypothesize that antibiotic efficacy correlated with bactericidal or toxin-suppressing properties of these agents. to investigate the possibility that penicillin is only bacteriostatic against c. perfringens, we ... | 1987 | 2882731 |
| age related variations of serum concentrations of normally occurring igg antibodies to clostridium perfringens. | in studies using indirect immunofluorescence igg antibodies to clostridium perfringens were found in sera from healthy adults. sera from 236 healthy children were examined. the normally occurring igg antibodies to c perfringens were found to have an age related variation. preliminary data suggest that they are not correlated to c perfringens alpha toxin. the antigen(s) against which the antibodies are directed is/are probably part of the cell wall, but its/their exact nature is not known. | 1987 | 2881950 |
| identification of tn4451 and tn4452, chloramphenicol resistance transposons from clostridium perfringens. | the recombinant plasmids pjir45 and pjir97 contain the chloramphenicol resistance determinants derived from the clostridium perfringens r plasmids pip401 and pjir27, respectively. escherichia coli cultures which harbored these recombinant plasmids rapidly became chloramphenicol sensitive when grown in the absence of chloramphenicol. the loss of resistance was associated with the loss of 6.2-kilobase (kb) segments from both plasmids. detailed restriction analysis of e. coli- and c. perfringens-de ... | 1987 | 2881919 |
| [clostridium perfringens type c enterotoxemia (necrotizing enteritis) of suckling pigs. 3. light and electron microscopic studies of the pathology and pathogenesis of experimental clostridium perfringens type c infection]. | 1986 | 2881527 | |
| [clostridium perfringens type c enterotoxemia (necrotizing enteritis) in suckling pigs. 2. light and electron microscopic studies of the pathology and pathogenesis of experimental clostridium perfringens type c toxin poisoning]. | 1986 | 2881526 | |
| [clostridium perfringens type c enterotoxemia (necrotizing enteritis) in suckling pigs. 1. study of the experimental production of disease by clostridium perfringens type c poisoning and infection (experimental set-up, clinical aspects, pathological findings)]. | 1986 | 2881525 | |
| [standardization and reproducibility of gas chromatographic research on the cellular fatty acids of microorganisms]. | 1987 | 2880882 | |
| cell surface sialylation of two human tumor cell lines and its correlation with their platelet-activating activity. | the relationship between cell surface sialylation and platelet-activating activity was studied in two tumor cell lines of human origin, the sknmc neuroblastoma line and the u87mg glioblastoma line. their platelet-activating activity was evaluated in two different experimental systems, one that measures platelet aggregation and the other that quantifies platelet thrombus formation on vascular subendothelium under flow conditions. our results demonstrate that, for the sknmc line, the loss of 30% o ... | 1987 | 2880661 |
| comparison of clindamycin, rifampin, tetracycline, metronidazole, and penicillin for efficacy in prevention of experimental gas gangrene due to clostridium perfringens. | gas gangrene caused by clostridium perfringens is associated with significant mortality and morbidity in spite of penicillin treatment. although prompt surgical debridement has been established as the primary therapeutic objective, additional studies are needed for determination of the optimal antimicrobial therapy. in a mouse model of gas gangrene caused by clostridium perfringens, clindamycin, metronidazole, rifampin, and tetracycline were all more efficacious than penicillin (p less than .05) ... | 1987 | 2879873 |