Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| emetine binding to ribosomes of entamoeba histolytica--inhibition of protein synthesis and amebicidal action. | 1979 | 226696 | |
| growth response of axenic entamoeba histolytica to hydrogen, carbon dioxide, and oxygen. | entamoeba histolytica required co2 for growth in axenic culture while growth was inhibited by h2. the organism was tolerant to 5% o2 in the gas phase and it was able to detoxify products of o2 reduction in the medium. the ameba did not require a negative oxidation-reduction potential for axenic growth. however, little or no free o2 was present in media exposed to 5% o2 in the gas phase. growth was improved by adding yeast extract to the medium. | 1979 | 226695 |
| microscopic observations on the filopodia of entamoeba histolytica. | living entamoeba histolytica trophozoites were examined by phase-contrast microscopy. intact critical point dried trophozoites were examined by transmission electron microscopy at an accelerating voltage of 1000 kv (hvem) and by scanning electron microscopy (sem). half and quarter micrometer thick sections of epoxy-embedded trophozoites were examined by hvem. many of the trophozoites of 2 strains examined had surface filopodia, 1 to over 100 micrometers in length. the cytoplasm of filopodia was ... | 1979 | 226693 |
| the epidemiology of entamoeba histolytica in a nigerian urban population. | microscopic examination of multiple faeces samples of 2 825 persons was carried out in the metropolitan lagos. overall prevalence of entamoeba histolytica was 11.2%. prevalence increased rapidly in younger age groups and there were no real differences between males and females. prevalence was high among families who ate together from the same plate, among those who ate with their fingers and among those who ate away from home. prevalence was not associated with type of water supply but was seemi ... | 1979 | 226490 |
| [current status of the chemotherapy of amebiasis]. | 1978 | 226448 | |
| [coproculture for "entamoeba histolytica". study of 170 cases examined at the clinic for tropical and infectious diseases at the universita di roma in the 1st semester of 1978]. | 1978 | 226012 | |
| antigenic analyses of two axenized strains of entamoeba histolytica by two-dimensional immunoelectrophoresis. | antigens in extracts of two strains of entamoeba histolytica (ht-31 and hk-9) were studied by two-dimensional immunoelectrophoresis (2d-iep) and 32 and 20 different antigenic components were detected, respectively. cross-reactions between heterologous systems showed a large number of shared antigens between the two strains. extracts were fractionated by sephadex g-200 chromatography. of the fractions obtained, fraction ii of both strains was found to have the most reactive antigenic components. ... | 1979 | 225959 |
| [histochemical studies of various enzymes in amoebae of the genus entamoeba: entamoeba histolytica (schaudinn, 1903), entamoeba invadens (rodhain, 1934) and entamoeba moshkovskii (tshalaia, 1941)]. | 1979 | 225891 | |
| immune response of monkeys (macaca mulatta) to amebic antigens. | 1979 | 225462 | |
| [immunization against entamoeba histolytica. current state and conclusive perspectives]. | 1979 | 225237 | |
| restoration of virulence of axenically cultivated entamoeba histolytica by cholesterol. | the lost pathogenicity of two strains of entamoeba histolytica, one isolated in 1924 and the other in 1967, grown in axenic culture for the past 5 and 6 years respectively, was restored by supplementing the culture medium with cholesterol through a number of transfers. the number of passages in the cholesterol-supplemented medium, necessary to restore a certain degree of pathogenicity of the two strains in hamsters, was proportional to the total time of in vitro cultivation of the strain, and no ... | 1978 | 224992 |
| experimental amebiasis. iv. amebal viruses and the virulence of entamoeba histolytica. | we have attempted to determine whether amebal viruses are capable of viral conversion of the virulence of entamoeba histolytical. amebae of little or no virulence for the newborn hamster liver were infected with viruses obtained from four amebal strains of different virulence. cultures of surviving amebae were increased in virulence by viruses from amebae of high, moderate, or low virulence, but decreased in virulence by the virus of the second most pathogenic amebal strain. sequential infection ... | 1979 | 223459 |
| [amebic liver abscess]. | 1979 | 223045 | |
| the serologic response of hamsters to experimental liver inoculations with entamoeba histolytica as measured by indirect hemagglutination test and enzyme linked immunosorbent assay. | results of testing 298 sera from hamsters inoculated in the liver with entamoeba histolytica and from 25 uninoculated controls by indirect hemagglutination (iha) and enzyme linked immunosorbent assay (elisa) showed that measurable amounts of antibody appeared as early as 5 days after inoculation, generally increased to a high level within 20 days, and persisted for the duration of the experiments (58 days). there was fair correlation between size of liver lesion and titers. strain differences in ... | 1979 | 222154 |
| riboflavin requirement for the cultivation of axenic entamoeba histolytica. | riboflavin was found to be essential for the cultivation of axenic entamoeba histolytica. this is the first demonstration of a flavin requirement by the organism. panmede, the principal source of flavins in the axenic medium, was treated with activated carbon to remove flavins. medium made with this flavin-deficient panmede, and supplemented with ribonucleic acid failed to support the multiplication of amebae in serial subculture, but did so when riboflavin was added. the concentration of ribofl ... | 1979 | 222153 |
| entamoeba histolytica: cytopathogenicity of intact amebae and cell-free extracts; isolation and characterization of an intracellular toxin. | 1979 | 221231 | |
| evaluation of parasitological and serological techniques in diagnosis of amoebiasis. | parasitological investigations carried out on 324 subjects of symptomatic and asymptomatic amoebiasis showed that formol-ether concentration was a superior technique than examination of 3 consecutive stool specimens by direct microscopy in cases of colitis, hepatitis and cyst passers. culture of pus yielded better results than the other techniques for examination of stool in cases of liver abscess. indirect haemagglutination test carried out with 238 sera from cases confirmed to be either positi ... | 1979 | 220760 |
| toxins of entamoeba histolytica. | 1979 | 220758 | |
| isolation of a cytotoxin-enterotoxin from entamoeba histolytica. | a heat-labile material with cytotoxic and enterotoxic activities was isolated from axenically cultivated entamoeba histolytica. the cytotoxin-enterotoxin was partially purified from the particulate-free supernatant of sonicated trophozoites by ammonium sulfate precipitation and gel filtration. cytotoxic activity, assayed on monolayers of hela or bhk-21 cells, was restricted to proteins that eluted in the molecular weight range of 25,000--35,000 daltons. cytotoxicity was demonstrated at protein c ... | 1979 | 220331 |
| entamoeba histolytica: flavins in axenic organisms. | 1979 | 220078 | |
| activation of the alternative pathway of complement by entamoeba histolytica. | a cytopathogenic effect was observed when entamoeba histolytica was exposed to human sera from individuals with no clinical history or laboratory evidence of amoebiasis. absorption studies showed that the effect was not due to natural antibodies. studies performed using ethylenediamine tetracetic acid (edta), cobra venom factor (cof) and heat-inactivation at 56 degrees c, indicated that the cytopathogenic effect was complement dependent. furthermore, by using ethylene glycol tetracetic acid (egt ... | 1978 | 219975 |
| localisation of thiamine pyrophosphatase within the cytoplasmic fine structure of trophozoites of entamoeba histolytica and e. invadens. | 1978 | 218887 | |
| comparison of fusobacterium sp. versus bacteroides sp. in the monoxenic cultivation of e. histolytica. | by means of the performed studies it was established that 6 fusobacteria strains enhanced the growth of e. histolytica while 4 bacteroides strains did not possess this property. | 1978 | 218400 |
| coproantibodies in intestinal amoebiasis using axenic entamoeba histolytica antigen. | 1978 | 217822 | |
| contribution to the problem of the so-called nonpathogenic amoebae in the intestine of man. | among 10,418 patients of a prague hospital, a plain infection with intestinal parasitic protozoans was identified in 1,319 persons (12.7%). of these, 3.5% were infested with giardia intestinalis, 0.3% with entamoeba histolytica forma minuta, 5.7% with endolimax nana. we evaluated the frequency of findings of protozoans in various clinical diagnoses. a statistically significant increase in frequency was recorded for e. nana in diagnoses of eosinophilia, giardiasis, amoebiasis and helminthiasis. ... | 1978 | 217807 |
| the effect of bisamidines of 2,6-diaminoanthraquinone on entamoeba histolytica infections in rats and hamsters. | bisamidines of 2,6-diaminoanthraquinone have demonstrated potent activity against cecal and hepatic entamoeba histolytica infections in rats and hamsters, respectively. a number of these compounds compared favorably, in overall drug efficacy, with metronidazole and other standard agents. | 1979 | 217714 |
| entamoeba histolytica: genetic control of susceptibility in chicken eggs. | 1979 | 217709 | |
| high rates of enteric protozoal infections in selected homosexual men attending a venereal disease clinic. | stool specimens from 89 high-risk, sexually active homosexual men were screened for enteric protozoal cysts. patients whose specimens contained nonpathogenic cysts were investigated further by examination of fresh purged specimens. a total of 27 of the initial specimens (30%) contained protozoal cysts. twenty-three patients (26%) harbored pathogens. there were 18 cases (20%) of amebiasis and 11 cases (12%) of giardiasis. six men (7%) had concurrent amebiasis and giardiasis. the presence of infec ... | 1978 | 217110 |
| [indications for the use of antibiotics for protozoan infections]. | 1978 | 216956 | |
| clonal growth of entamoeba histolytica and other species of entamoeba in agar. | this is the first description of a method for growing axenized entamoeba histolytica as colonies from single cells in agar suspension. factors affecting the efficiency of colony formation included unknown characteristics of the amebal strain, age of cells used, and the type and concentration of agar employed. colony formation was dependent upon filling deep vessels (culture tubes or tissue culture flasks) with agar, probably to maintain reduced oxygen tension, and upon solidifying the agar rapid ... | 1978 | 216801 |
| [azotized emetine analogues: synthesis and evaluation of the anti-amebic and anti-tumoral action of aza-3 emetine and attempted synthesis of aza-2 emetine]. | the synthesis of the n,n-bis-(acetylhomoveratrylamido)-1-hydroxymethyl and 1-carboxypropylamines (iii a) and (iii b) is described. until now, these molecules could not been cyclized to the corresponding isoquinoline compounds. an attempted synthesis of 3-azaemetine by condensing homoveratrylamine with acetonedicarboxylic acid or its esters did not yield the expected diamide (xiv). however, by subjecting 1,3-bis-(1,2,3,4-tetrahydro-6,7-dimethoxy-1-isoquinolyl)acetone (xv) to a reducing aminoalkyl ... | 1978 | 216576 |
| the interaction between entamoeba histolytica and syphacia obvelata infection in mice. | mice naturally infected with syphacia obvelata were challenged intracaecally with entamoeba histolytica trophozoites. they showed a higher level of amoebic infection than mice without worms. mice with the heaviest syphacia infection were the most susceptible to amoebic infection. it appears that a concomitant infection with helminthic parasites residing in the caecum alters the outcome of an amoebic infection. | 1978 | 216325 |
| amebic dysentery. animal model: experimental entamoeba histolytica infection in the germfree guinea pig. | 1979 | 216267 | |
| [medical aspects of work in tropical countries]. | 1978 | 214964 | |
| the differentiation of invasive and non-invasive entamoeba histolytica by isoenzyme electrophoresis. | cultures of 85 stocks of entamoeba histolytica were compared by electrophoretic patterns of three enzymes: glucosephosphate isomerase, phosphoglucomutase and l-malate: nadp+ oxidoreductase (oxaloacetate-decarboxylating). four groups of e. histolytica stocks have been distinguished. 18 of these stocks were derived from individual patients with clinical amoebiasis and all of these were of one enzyme group, which did not occur among the other stocks. | 1978 | 214906 |
| [effect of bacteria on the virulence of an entamoeba histolytica strain long growing in the presence of metronidazole]. | 1978 | 214693 | |
| [imported protozoan and worm infections]. | 1978 | 214675 | |
| susceptibility of various strains of mice to entamoeba histolytica. | 1978 | 214540 | |
| immunologic studies on hamsters infected with entamoeba histolytica. | the serologic and cell-mediated immune responses of hamsters exposed to 2 strains of entamoeba histolytica (hm-1 and hm-19) were evaluated by a series of in vitro tests. the pathogenicity of the 2 strains was evaluated in terms of their ability to produce liver abscesses and spleen enlargement. antibody response was evaluated by the indirect hemagglutination test. the cellular immune response was assayed by increased dna synthesis by lymphocytes and migration inhibition of macrophages. | 1978 | 214539 |
| the presence of liver auto-antibodies induced by entamoeba histolytica in the sera from both naturally infected humans and immunized rabbits. | auto-antibodies against normal human liver have been detected in the sera of humans with highly positive indirect hemagglutination (iha) amebiasis titers and with clinically-proven amebic liver abscess. sera of amebiasis patients and rabbits immunized with killed entamoeba histolytica were tested for anti-amebic antibodies by the iha test and for auto-antibodies by the complement fixation test, using the antigens prepared from extracts of human liver and rabbit liver. a direct correlation was fo ... | 1978 | 213983 |
| hepatic amebiasis in spider monkeys. | in the past, investigators have been able to produce hepatic amebiasis in laboratory animals only by direct introduction of parasites into the liver or its vasculature, or by other artificial manipulations. a natural model of human visceral amebiasis has been lacking. we document an extensive outbreak of amebic dystentery which took place in a colony of spider monkeys; severe hepatic abscesses occurred in many animals. the spider monkey is highly susceptible to infection with entamoeba histolyti ... | 1978 | 213982 |
| experimental amebiasis. iii. a rapid in vitro assay for virulence of entamoeba histolytica. | a rapid and simple assay for cytopathogenicity of axenically cultivated entamoeba histolytica has been developed employing baby hamster kidney (bhk) or mouse 3t3 cells conventionally tissue cultured. three of the twelve amebal strains tested produced total destruction of the bhk cell monolayer in 2--3 hours, and these three strains are the three most virulent strains for the newborn hamster liver. two additional strains were of moderate cytopathogenicity in vitro and of moderate virulence in viv ... | 1978 | 213981 |
| [technics of measuring the motive force of very small ameboid cells]. | a technic have been perfected which measures directly the motive force of very small ameboid cells and indicates the tropism of the amoeba. by using this technic, the motive force of entamoeba histolytica was measured. it varied between +/- 1.5 x 10-4 dyne. we have also shown chimiotactism in entamoeba histolytica. the metabolic coumpound excreted by this cell induced negative tropism. | 1978 | 213820 |
| [invasion of the mucosa of the colon by entamoeba histolytica. recent observations]. | 1978 | 213335 | |
| a new medium for the axenic cultivation of entamoeba histolytica and other entamoeba. | 1978 | 212851 | |
| serial measurement of total and parasite-specific ige in an african population infected with entamoeba histolytica. | 1978 | 212850 | |
| biomedical survey in the moluccas, indonesia. | 1978 | 212835 | |
| inorganic pyrophosphatase and nucleoside diphosphatase in the parasitic protozoon, entamoeba histolytica. | 1978 | 212030 | |
| achievements and obstacles in research for new anti-amoebic drugs. | 1978 | 211970 | |
| [a new hemagglutination method to determine antibodies against entamoeba histolytica]. | a passive hemagglutination method useful for the determination of antibodies against entamoeba histolytica is described. the e. histolytica antigen is attached to group "o" human red blood cells by means of crcl3. the specificity and sensitivity of the crcl3 method was compared with other hemagglutination methods, i.e., tannic acid method and a commercial preparation, as well as counterimmunoelectrophoresis. the crcl3 method showed a reactivity similar to the tannic acid hemagglutination techniq ... | 1978 | 211969 |
| [determination of an anti-ameba antigen in the feces by the elisa method (enzyme-linked immunosorbent assay) for the identification of entamoeba histolytica]. | 1978 | 211968 | |
| entamoeba histolytica: iron and nutritional immunity. | 1978 | 211967 | |
| [induction of protective anti-ameba immunity with new antigens in the suckling hamster. b. antigenic material]. | 1978 | 211966 | |
| [induction of protective anti-ameba immunity with new antigens in the suckling hamster. a. introduction]. | 1978 | 211965 | |
| [immunite reaction of hamsters infected or immunized with entamoeba histolytica]. | the immune response in hamsters either infected or immunized with entamoeba histolytica was studied. the response was tested by determination of antibodies by the counterimmunoelectrophoresis technique and by lymphocyte transformation induced by the amebic antigen. the results obtained showed differences among both groups when the ability of amebic antigen to induce 3h-thymidine incorporation of lymphocyte cultures is compared. however, the response of lymphocyte cultures to concanavalin a was s ... | 1978 | 211964 |
| [activity of the alternative complement pathway by various axenic strains of entamoeba histolytica]. | the activation of the alternative pathway of complement by axenic cultures of e. histolytica is reported. the activation is carried out in different proportions by several axenic strains, i.e., hk9:nih, hm1:imss, hm2:imss and hm3:imss; the end result consists of a destruction of the parasite as well as consumption of the serum complement. the participation of the alternative pathway was studied in the presence of egta and mg++, zymosan, heat-inactivation at 50 degree c for 20 min and immunoelect ... | 1978 | 211963 |
| [serology with polysaccharides of entamoeba histolytica]. | the immunochemical activity of a polysaccharidic antigen derivated from e. histolytica was studied. this polysaccharide was tested by means of counterimmunoelectrophoresis (cie) and radial immunodiffusion (idr) against sera from patients with diagnosis of intestinal and hepatic amebiasis. in order to perform a test most significative to the problem sera, these were submitted previously to passive hemagglutination, cie and idr with total ameba antigen. in hepatic amebiasis it can be observed that ... | 1978 | 211962 |
| [ultramicroscopic study of the invasion of the mucosa of the colon by entamoeba histolytica in axenic cultures]. | animal models have been utilized to study experimentally produced intestinal amebiasis. the guinea pig has been the animal of choice to study the mechanisms by which trophozoites of e. histolytica produce colonic lesions. the purpose of this study was to produce lesions in the colon of newborn guinea pigs by the injection of e. histolytica, hm-1 imss, strain grown axenically, and to characterize the early changes with the light and electron microscopes. a total of 35 guinea pigs, one to two days ... | 1978 | 211961 |
| [interaction between entamoeba histolytica and the cecal epithelium in the guinea pig]. | the early interaction between e. histolytica trophozoites and the intestinal mucosa was studied by light and electron microscopy using sheets of isolated epithelium of the guinea pig colonic mucosa. the integrity of the isolated epithelium was confirmed by scanning electron microscopy. after 30 minutes of interaction, the amebas adhered first, and penetrated subsecuently the epithelium of the colon at the interglandular regions. the results suggest that the trophozoites of e. histolytica invade ... | 1978 | 211960 |
| [an in vitro model for the quantitative study of e. histolytica virulence]. | an experimental model for the quantitative study of the cytopathic effect of e. histolytica is described. confluent cultures of the mdck cell line, which form a monolayer with morphological and electrophysiological features of an epithelium were used. trophozoites of the strains hm1:imss, hm2:imss and hm3:imss, axenic produced variable degrees of cytopathic effects of mdck cultures each one characteristic for a given strain. the degree of damage induced by the different strains of amebas was mea ... | 1978 | 211959 |
| restoration of virulence of axenically-cultivated entamoeba histolytica by cholesterol. | the lost pathogenicity of two strains of entamoeba histolytica, one isolated in 1924 and the other in 1967, grown in axenic culture for the past five and six years respectively, was restored by supplementing the culture medium with cholesterol through a number of transfers. the number of passages in the cholesterol supplemented medium, necessary to restore a certain degree of pathogenicity of the two strains in hamsters, was proportional to the total time of in vitro cultivation of the strain, a ... | 1978 | 211958 |
| clonal growth of entamoeba in agar: some applications of this technique to the study of their cell biology. | a new technique for growing single entamoeba trophozoites into colonies in agar has been developed. this method depended upon axenic cultivation and utilized diamond's new tyi-s-33 medium. the present paper describes several different types of experiment which have utilized the agar technique. a) cloning. the isolation of clones from agar way easy and successful. clones of the hm-1 strain did not differ in virulence for newborn hamster liver or in colony morphology or colony forming efficiency. ... | 1978 | 211957 |
| cytotoxicity of a cell free extract of entamoeba histolytica. | previous attempts to demonstrate toxicity of cell-free extracts prepared from axenically cultivated e. histolytica have proved unsuccessful. we report here the successful production of cytopathic effect by cell-free extracts of two strains (hm-1 and hk-9) of e. histolytica in tissue culture monolayers. the cytotoxic effect demonstrated was sensitive to serum in the assay medium. failure of past investigations to demonstrate cytotoxic activity may be explained by the presence of serum in the assa ... | 1978 | 211956 |
| [culture and establishment of new mexican strains of entamoeba histolytica]. | mixed and monoxenic cultures of rectal exudates from three patients with amebiasis of colon, and of feces from three asymptomatic carriers of entamoeba histolytica obtained after administration of a saline cathartic, and from a case of exudate of the perianal cutaneous lesion border produced by this parasite, were done. the culture media used were for: mixed culture, boeck and drbohlav modified culture and, for monoxenic culture, diamond's monophasic tp-s-1 medium without vitamin mixture and usi ... | 1978 | 211955 |
| production of cecal lesions in newborn guinea pigs with axenically cultivated entamoeba histolytica. | the production of cecal lesions with axenically cultivated entamoeba histolytica in an experimental animal is reported for the first time. forty-three newborn guinea pigs (one to five days old) were inoculated via laparatomy with 2.5 x 10(5) to 2 x 10(6) strain hm-1-imss amebae. of 23 animals surviving to the 7th postinoculation day all showed cecal lesions at necropsy regardless of the age of the animal when used or the number of amebae received. ten animals had lesions visible to the unaided e ... | 1978 | 211954 |
| [phagocytosis of human erythrocytes by entamoeba histolytica. quantitative study]. | the degree of eythrophagocytosis of two recently isolated strains of e. histolytica was measured by microscopic examination. amebas isolated from a patient with amebic rectocolitis (strain hm22:imss, monoxenic) ingested human red blood cells faster and in larger numbers than trophozoites isolated from an asymptomatic carrier (hm27:imss, monoxenic). the results suggest that an increased rate of phagocytosis could be one of the surface properties characteristic of the invasive strains of e. histol ... | 1978 | 211953 |
| [identification of entamoeba histolytica membrane antigens with antibodies of amebiasis patients]. | the specificity of sera from patients with amoebiasis was assayed on trophozoites of e. histolytica hk9, hm15 and hm2 cultured axenically and monoxenically. glutaraldehyde fixed cell were incubated with samples of serum, the excess of igs was washed out and the presence of unbound material of specifically bound antibodies was measured with radiolabeled protein a from staphylococcus aureus. a positive reaction with hk9 was observed in 18 per cent of the 66 sera tested. an immune crossreaction res ... | 1978 | 211952 |
| the development and standaridization of an elisa method for the detection of entamoeba histolytica antigens in fecal samples. | recognizing the difficluties associated with the microscopic detection and identification of entamoeba histolytica in fecal samples, an immunochemical method of detection of e. histolytica antigens in fe-es has been developed. for a number of reasons, e.g., the known fragility of e. histolytica trophozoites, it was felt that intestinal amebiasis would be associated with detectable levels of e. histolytica anti-ens in feces from infected patients. a technique for harvesting amebic antigens in fec ... | 1978 | 211951 |
| surface ultrastructure of entamoeba histolytica; a study by high voltage transmission electron microscopy (hvtem) and scanning electron microscopy (sem). | 1978 | 211950 | |
| variable toxicity within the hk 9 strain of entamoeba histolytica. | 1978 | 211949 | |
| amebal viruses and the virulence of entamoeba histolytica. | 1978 | 211948 | |
| [effects of nordihydroguairetic acid and ethanol on the growth of entamoeba invadens]. | the effects of the alcoholic extract (resin) obtained from larrea tridentata, of nordihydroguiaretic acid (ndga) and of ethanol over axenic cultures of entamoeba invadens pz strain were studied. the activity of these substances was demonstrated in growht curves. inocula of 1-3 x 10(5) trophozites by milliliter in culture tubes containing 11 ml of tp-s-1 medium were done, and incubated at 25 degree c. in each experiment the follwoing was done: a) tipical growth curve as a control, b) curve with t ... | 1978 | 211947 |
| [cytopathogenic activity of extracts e. histolytica trophozoites]. | previous studies have not shown any correlation between enzyme activities and pathogenicity of entamoeba histolytica. the activities analyzed were selected without having proved their involvement in the cytopathogenicity. we are using the reverse approach to study the cytopathogenic factors, i.e., to detect and characterize first the cytopathic effect of amebic extracts on animal cells, and subsequently to try to identify the components involved and to explore their correlation with amebic virul ... | 1978 | 211946 |
| [immunochemistry of immunogenic proteins of entamoeba histolytica]. | entamoeba histolytica (hk-9: imss) trophozoites were separated from the culture medium and washed and submitted to the effects of ultrasound during 10 minutes. after 10,000 rpm x 30 centrifugation, the supernatant fluid was separated in four fractions by sephadex g-200 gel chromatography. these fractions were lyophilized, redisolved in a lesser volume and dyalized against isotonic saline solution. antigen-antibody precipitant reactions between each of them and two rabbit antiserums were demonstr ... | 1978 | 211945 |
| uptake of selected pyrimidines by axenically grown entamoeba histolytica. | this study was conducted to determine the mode(s) of uptake of selected pyrimidine bases and nucleosides by axenically grown entamoeba histolytica. two-minute uptake studies with 3h-labeled compounds showed the following: 1) uridine and cytidine are taken up, in part, by a carrier mediated system; 2) uracil, cytosine, thymine and thymidine enter amebae via passive diffusion; both cytidine and uridine are taken up by passive diffusion when present at exogenous concentration greater than 500 mum; ... | 1978 | 211944 |
| [amino acid composition in total proteins of strains of entamoeba histolytica in axenic cultures]. | in this paper we detail the amino acid composition of total protein hydrolyzates from trophozoites of two entamoeba histolytica strains (hk-9:nih and hm-2:imss) cultured axenically in tp-s-1 medium. since we obtained nearly identical results in both strains, amino acid patterns may constitute an additional taxonomic criterion of the species. our data are strikingly different from those found previously by other workers with total proteins from hk-9:nih trophozoites cultured in a bacteria-conditi ... | 1978 | 211943 |
| isolation and sedimentation analysis of rna from entamoeba histolytica. | 1978 | 211942 | |
| [instability of the dna content in entamoeba histolytica]. | dna content was repeatedly determined during a 10-month period on axenically cultured tropho-zoites from three strains (hk-9:nih, hm-2:imss and hm-3:imss) and a clone of hk-9:nih strain of entamoeba histolytica, by means of the diphenylamine reaction. average dna content was more or less variable in all of them. according to their extent, variations were: 1) large (almost four times in strain hm-2:imss), or 2) small (1.5 times in the clonal isolate). with this relatively small sample, three stat ... | 1978 | 211941 |
| [ultramicroscopic structure of the cyst wall of entamoeba invadens, e. histolytica and e. coli]. | the ultrastructure of the wall of e. invadens cysts induced in axenic cultures was compared by transmission electron microscopy of thin sections, with that of e. histolytica and e. coli cysts obtained from feces of human asymptomatic carriers. the cyst wall of the three species studied had similar ultrastructural features; in all, the wall was composed mainly by the aggregation of filaments adjacent to the plasma membrane. the cytoplasmic components which might be involved in the synthesis and d ... | 1978 | 211940 |
| experimental muscular amebiasis in hamsters as a biological model. | trophozoites of entamoeba histolytica maintained in vitro in pavlova's medium were inoculated by deep intramuscular injection into the proximal left hindleg of hamsters. thioglycollate medium was utilized as a successful vehicle to induce the infection. the invasion of the muscular tissue by the vegetative forms caused the formation of abscesses with great destruction of muscular fibers. the lesions were limited to the muscular tissue of the femoral area. the number of trophozoites, the medium o ... | 1978 | 211747 |
| growth pattern of axenic entamoeba histolytica in different sera and the antiamoebic activity of specific antisera. | 1978 | 211082 | |
| indirect fluorescent antibody test in amoebiasis using axenic entamoeba histolytica. | 1978 | 211081 | |
| vaccination against hepatic amebiasis in hamsters. | 1978 | 210268 | |
| pathogenicity of entamoeba histolytica--effect of cholesterol on the virulence of strains of amoebae. | 1978 | 210121 | |
| modified stuart medium--a transport and maintenance medium for entamoeba histolytica. | 1978 | 210120 | |
| entamoeba histolytica: uptake of purine bases and nucleosides during axenic growth. | 1978 | 210037 | |
| [acute intestinal amebiasis treated with aminosidine]. | a clinical-therapeutic assay carried out at the "william soler" pediatric teaching hospital in children admitted due to an acute diarrheic syndrome and treated with aminosidine sulfate is presented. histolytic ameba is still an element to be considered in the origin of gastroenteritis. the therapeutic action of aminosidine is evaluated. | 1977 | 209805 |
| new and old agents in diarrhea: a prospective study of an indigenous adult african population. | we conducted a prospective study 77 indigenous african adults with acute diarrhea seeking care at the major hospital in nairobi, kenya, to determine the major pathogens responsible for this syndrome in adults. fecal and blood specimens were collected and examined for enteric bacterial pathogens, viruses, and parasites. in 13 (26%) inpatients and 11 (49%) outpatients shigella was found, and heat-labile and heat-stable forms of enterotoxigenic escherichia coli were found in 9 (18%) inpatients and ... | 1978 | 209697 |
| an evaluation of the absorption spectra of antigens from entamoeba histolytica (ibadan strains). | by means of chromatography different fractions of antigens from entamoeba histolytica (ibadan strains) have been separated. all the different fractions have been tested for antigenic reactivity using agar gel diffusion technique. the intact antigen, as well as, the three antigenically reactive fractions were found to have ultra-violet absorption spectra in the region 258 and 278 millimicron. by virtue of this physico-chemical property amoebic extract(s) was found to simulate a mononucleotide deo ... | 1977 | 209678 |
| [apropos of a case of amebic liver abscess. apropos of one case]. | 1978 | 209260 | |
| biomedical survey in irian jaya (west irian), indonesia. | a biomedical survey was conducted in several areas of irian jaya, indonesia in july 1972 in association with an investigation of reports of a cholera outbreak. stool specimens, blood smears and sera were collected and examined for evidence of parasitic as well as other infectious diseases. a total of 114 stools were examined and the most commonly found intestinal parasites were trichuris trichiura (94%), ascaris lumbricoides (74%), hookworm (58%), entamoeba coli (15%), endolimax nana (8%), entam ... | 1977 | 208184 |
| [update on amebiasis. physiopathology]. | 1978 | 208130 | |
| synthesis and amoebicidal activity of novel substituted quinolines. | 1978 | 207641 | |
| presence of prostaglandins a & e in entamoeba hystolytica. | 1977 | 207640 | |
| detection & characterization of humoral antibodies from amebic immune sera. | 1977 | 207638 | |
| entamoeba histolytica, e. invadens, and e. moshkovskii: fluctuations of the dna content of axenic trophozoites. | 1978 | 207550 | |
| entamoeba histolytica and homo sapiens. | blind acceptance of the dicta of the great has led to much confusion as to the relationship between amoeba and man. a review of the mistakes of the past may lead to a better appreciation of the present, and higher hopes for the future. an hypothesis is presented. | 1978 | 207506 |
| a simple technique for preparing clone cultures of entamoeba histolytica. | 1978 | 206992 | |
| electrophoretic isoenzyme patterns of entamoeba histolytica and entamoeba coli. | cultures of 14 stocks of entamoeba histolytica and one only of entamoeba coli were compared by electrophoretic patterns of three enzymes: glucose-phosphate isomerase, phosphoglucomutase and l-malate: nadp+ oxidoreductase (oxaloacetate-decarboxylating). easily distinguished patterns divided e. histolytica into three groups, whilst a distinctly different pattern for e. coli was also seen. | 1978 | 206991 |
| haematogenous amoebic lung abscess. a case report. | left upper lobe amoebic lung abscess without established hepatic amoebiasis in a young black man is described and the pathogenesis is briefly discussed. | 1978 | 206979 |