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production of acyl-homoserine lactone quorum-sensing signals by gram-negative plant-associated bacteria.many gram-negative bacteria regulate expression of specialized gene sets in response to population density. this regulatory mechanism, called autoinduction or quorum-sensing, is based on the production by the bacteria of a small, diffusible signal molecule called the autoinducer. in the most well-studied systems the autoinducers are n-acylated derivatives of l-homoserine lactone (acyl-hsl). signal specificity is conferred by the length, and the nature of the substitution at c-3, of the acyl side ...19989805399
phylogenetic position of phytopathogens within the enterobacteriaceae.the almost complete 16s rdna sequences of twenty nine plant-associated strains, representing species of the genera erwinia, pantoea and enterobacter were determined and compared with those of other members of the enterobacteriaceae. the species of the genus erwinia may be divided into three phylogenetic groups. cluster i represents the true erwinias and comprises e. amylovora, e. mallotivora, e. persicinus, e. psidii, e. rhapontici and e. tracheiphila. we propose to unite the species of cluster ...19989779605
colonization of wheat roots by an exopolysaccharide-producing pantoea agglomerans strain and its effect on rhizosphere soil aggregationthe effect of bacterial secretion of an exopolysaccharide (eps) on rhizosphere soil physical properties was investigated by inoculating strain nas206, which was isolated from the rhizosphere of wheat (triticum durum l.) growing in a moroccan vertisol and was identified as pantoea aglomerans. phenotypic identification of this strain with the biotype-100 system was confirmed by amplified ribosomal dna restriction analysis. after inoculation of wheat seedlings with strain nas206, colonization incre ...19989758793
the gene for indole-3-acetyl-l-aspartic acid hydrolase from enterobacter agglomerans: molecular cloning, nucleotide sequence, and expression in escherichia coli.a 5.5-kb dna fragment containing the indole-3-acetyl-aspartic acid (iaa-asp) hydrolase gene (iaasph) was isolated from enterobacter agglomerans strain gk12 using a hybridization probe based on the n-terminal amino acid sequence of the protein. the dna sequence of a 2.4-kb region of this fragment was determined and revealed a 1311-nucleotide orf large enough to encode the 45-kda iaa-asp hydrolase. a 1.5-kb dna fragment containing iaasph was subcloned into the escherichia coli expression plasmid p ...19989747708
characterization of pucd5000 involved in pink disease color formation by pantoea citrea.pantoea citrea, the causal agent of pink disease of pineapple, harbors a cryptic plasmid of 5229 bp. designated pucd5000. on the basis of nucleotide and amino acid sequence analyses, pucd5000 contains both replication and mobilization loci (bom and mobcabd) that are similar to those in plasmids psw100 and psw200 in pantoea stewartii and pec3 in erwinia carotovora subsp. carotovora. the survival of p. citrea on pineapple does not depend on pucd5000. however, full pink coloration development, whic ...19989735319
microbial communities of printing paper machines.the microbial content of printing paper machines, running at a temperature of 45-50 degrees c and at ph 4.5-5, was studied. bacteria were prevalent colonizers of the machine wet end and the raw materials. a total of 390 strains of aerobic bacteria were isolated and 86% of these were identified to genus and species by biochemical, chemotaxonomic and phylogenetic methods. the most common bacteria found at the machine wet end were bacillus coagulans and other bacillus species, burkholderia cepacia, ...19989717292
identification of a new site for ferrichrome transport by comparison of the fhua proteins of escherichia coli, salmonella paratyphi b, salmonella typhimurium, and pantoea agglomerans.the fhua genes of salmonella paratyphi b, salmonella typhimurium, and pantoea agglomerans were sequenced and compared with the known fhua sequence of escherichia coli. the highly similar fhua proteins displayed the largest difference in the predicted gating loop, which in e. coli controls the permeability of the fhua channel and serves as the principal binding site for the phages t1, t5, and phi80. all the fhua proteins contained the region in the gating loops required in e. coli for ferrichrome ...19989683481
a pathogenicity gene isolated from the ppath plasmid of erwinia herbicola pv. gypsophilae determines host specificity.the host range of the gall-forming bacterium erwinia herbicola pv. gypsophilae (ehg) is restricted to the gypsophila plant whereas e. herbicola pv. betae (ehb) incites galls on beet as well as gypsophila. the pathogenicity of ehg and ehb was previously shown to be dependent on a plasmid (ppath). transposon mutagenesis was used to generate mutants on the cosmid pla150 of the ppath from ehg824-1. a cluster of nonpathogenic mutations flanked by two is1327 elements was identified on a 3.2-kb ndei dn ...19989675891
a negative regulator mediates quorum-sensing control of exopolysaccharide production in pantoea stewartii subsp. stewartii.classical quorum-sensing (autoinduction) regulation, as exemplified by the lux system of vibrio fischeri, requires n-acyl homoserine lactone (ahl) signals to stimulate cognate transcriptional activators for the cell density-dependent expression of specific target gene systems. for pantoea stewartii subsp. stewartii, a bacterial pathogen of sweet corn and maize, the extracellular polysaccharide (eps) stewartan is a major virulence factor, and its production is controlled by quorum sensing in a po ...19989636211
midgut bacteria in anopheles gambiae and an. funestus (diptera: culicidae) from kenya and mali.field studies in kenya and mali investigated the prevalence of bacteria in the midguts of malaria vectors, and the potential relationship between gram-negative bacteria species and plasmodium falciparum sporozoites. midguts were dissected from 2,430 mosquitoes: 863 anopheles funestus giles and 1,037 an. gambiae s.l. giles from kenya, and 530 an. gambiae s.l. from mali. an. funestus had a higher prevalence of gram-negative bacteria (28.5%) compared with an. gambiae collected in kenya and mali (15 ...19989615538
molecular recognition of siderophores: a study with cloned ferrioxamine receptors (foxa) from erwinia herbicola and yersinia enterocolitica.the outer membrane receptor for ferrioxamines (foxaerw) of erwinia herbicola (pantoea agglomerans) was cloned from a cosmid gene bank and partially sequenced. a comparison of the partial amino acid sequence of foxaerw with the amino acid sequence of foxayer from yersinia enterocolitica revealed a high sequence homology. a functional analysis of foxaerw and foxayer receptors cloned into a fhu-negative background (hk97) revealed that ferrioxamines are recognized at very low concentrations (< 10 pm ...19989542066
high affinity binding of albicidin phytotoxins by the alba protein from klebsiella oxytoca.albicidins are a family of phytotoxins and antibiotics which play an important role in the pathogenesis of sugarcane leaf scald disease. the alba gene from klebsiella oxytoca encodes a protein which inactivates albicidin by heat-reversible binding. albicidin ligand binding to a recombinant alba protein, purified by means of a glutathione s-transferase gene fusion system, is an almost instant and saturable reaction. kinetic and stoichiometric analysis of the binding reaction indicated the presenc ...19989493391
first report of a human isolate of erwinia persicinus.erwinia persicinus was first described in 1990 after being isolated from a variety of fruits and vegetables, including bananas, cucumbers, and tomatoes. in 1994, it was shown to be the causative agent of necrosis of bean pods. we now report the first human isolate of e. persicinus. the strain was isolated from the urine of an 88-year-old woman who presented with a urinary tract infection. by the hydroxyapatite method, dna from this strain was shown to be 94.5% related at 60 degrees c and 86% rel ...19989431957
the gene for albicidin detoxification from pantoea dispersa encodes an esterase and attenuates pathogenicity of xanthomonas albilineans to sugarcane.albicidin phytotoxins are pathogenicity factors in a devastating disease of sugarcane known as leaf scald, caused by xanthomonas albilineans. a gene (albd) from pantoea dispersa has been cloned and sequenced and been shown to code for a peptide of 235 amino acids that detoxifies albicidin. the gene shows no significant homology at the dna or protein level to any known sequence, but the gene product contains a gxsxg motif that is conserved in serine hydrolases. the albd protein, purified to homog ...19979275238
anti-tumor effect of lipopolysaccharide by intradermal administration as a novel drug delivery system.we examined the antitumor effect of lipopolysaccharide extracted from pantoea agglomerans, a gram-negative bacterium, using intradermal administration on murine syngeneic tumors, meth a fibrosarcoma, mh134 hepatoma and lewis lung (ll) carcinoma. the latter two tumors are known to be relatively low in immunogenicity, highly metastatic and to have low sensitivity to biological response modifiers. although the intradermal administration of lpsp had a significantly suppressive effect on the growth o ...19979216680
antitumor mechanism of intradermal administration of lipopolysaccharide.we earlier demonstrated that 50% of the lethal dose of lipopolysaccharide (lps) from pantoea agglomerans given by the intradermal (i.d.) route is about 300 times greater than that given by the intravenous (i.v.) route, and that 400 micrograms/kg of lps administered i.d. significantly suppressed metastasis whereas administered i.v., it did not. to learn the specific mechanism involved in this i.d. administration, the fate of lps at the skin following administration and the concurrent production o ...19979216652
structural characterization of lipid a obtained from pantoea agglomerans lipopolysaccharide.lipopolysaccharide isolated from pantoea agglomerans showed higher priming and triggering activities for macrophages in terms of tumor necrosis factor production than other lipopolysaccharides. to identify the difference in biological activities of lipopolysaccharide of pantoea agglomerans from other lipopolysaccharides on the basis of structure, we determined the structure of the lipid a part, which is the biological center of lipopolysaccharides, by quantitative analysis, nuclear magnetic reso ...19979141664
mechanisms of biocontrol by pantoea dispersa of sugar cane leaf scald disease caused by xanthomonas albilineans.albicidins, a family of phytotoxins and antibiotics produced by xanthomonas albilineans, are important in sugar cane leaf scald disease development. the albicidin detoxifying bacterium pantoea dispersa (syn. erwinia herbicola) sb1403 provides very effective biocontrol against leaf scald disease in highly susceptible sugar cane cultivars. the p. dispersa gene (albd) for enzymatic detoxification of albicidin has recently been cloned and sequenced. to test the role of albicidin detoxification in bi ...19979134719
mechanistic analysis of high antitumor effect of intradermal administration of lipopolysaccharide from pantoea agglomerans.lipopolysaccharide from pantoea agglomerans (lpsp) has a remarkably high antitumor activity even against poorly immunogeneic tumors when given by intradermal injection combined with cyclophosphamide (cy). we have extended this study to gain an insight into the mechanism of this antitumor effect, and especially into the induction of cell mediated immunity. in immunohistological studies, extensive necrosis and marked infiltration of the inflammatory cells at the tumor were observed after intraderm ...19969013473
identification and characterization of a pantoea citrea gene encoding glucose dehydrogenase that is essential for causing pink disease of pineapple.pantoea citrea, a member of the family enterobacteriaceae, causes pink disease of pineapple, whose symptom is characterized by the formation of pink to brown discolorations of the infected portions of the pineapple fruit cylinder upon canning. molecular genetic approaches were applied to elucidate the mechanism responsible for this fruit discoloration. a p. citrea mutant strain, cmc6, defective in its ability to cause pink disease and fruit discoloration, was generated by nitrosoguanidine mutage ...19978979341
biochemical characteristics of enterobacter agglomerans and related strains found in buckwheat seeds.thirty strains of bacteria were randomly isolated and identified from buckwheat seeds. the phenotypic characteristics of these strains agree well with those of the enterobacter agglomerans-erwinia herbicola complex. on the basis of the difference in indole production and gas production from d-glucose, the isolates were divided into 3 phenotypic groups, viz. i, ii and iii. twenty two strains were in phenotypic group 1, which is negative for indole production and gas production from d-glucose, and ...19968854178
distribution of diaminopropane and acetylspermidine in enterobacteriaceae.polyamines of 97 strains (60 species) belonging to 18 genera of the family enterobacteriaceae were determined by high performance liquid chromatographic analysis. in addition to putrescine and cadavarine, diaminopropane was widely distributed in enterobacteriaceae and almost ubiquitously within enterobacter, pantoea, erwinia, leminorella, proteus, leclercia, morganella, klebsiella, hafnia, rahnella, serratia, and tatumella species and sporadically within citrobacter, escherichia, moellerella, pr ...19968742354
significance of the c-terminal domain of erwinia uredovora ice nucleation-active protein (ina u).ice nucleation-active (ina) proteins of bacterial origin comprise three distinct domains, i.e., n-terminal (n-), central repeat (r-), and c-terminal (c-) domains, among which the r-domain is essential, and its length may be correlated with the ice nucleation activity. in addition, the short c-terminal domain of about 50 amino acid residues is indispensable for the activity. using the ina u protein of erwinia uredovora, we carried out precise mutational analyses of its c-terminus. the ice nucleat ...19958720147
chemotaxis of alveolar macrophages and neutrophils in response to microbial products derived from organic dust.mechanisms of chemotaxis of alveolar macrophages (ams) and neutrophils (pmns) in response to microbial products derived from organic dust were studied using the blindwell chemotaxis chamber technique. seven different known etiological agents causing respiratory symptoms were used for experiments: cell extract and endotoxin from pantoea agglomerans (synonyms: erwinia herbicola, enterobacter agglomerans), cell extracts from thermoactinomyces vulgaris and aspergillus fumigatus, protease from bacill ...19958705013
intradermal administration of lipopolysaccharide in treatment of human cancer.lipopolysaccharide (lps) has been recognized as a potent antitumor agent in animal tumor models; however, its use in human cancer therapy has been limited to only one trial, in which lps from salmonella was given intravenously. it was not very successful because of poor tumor response and was also toxic. we originally developed lps prepared from pantoea agglomerans (lpsp), and this was a well-purified, small-molecular-mass (5 kda) agent. we chose intradermal rather than intravenous administratio ...19968665574
use of continuous culture to screen for lipase-producing microorganisms and interesterification of butter fat by lipase isolates.the continuous cultivation technique was used to investigate the screening for lipase-producing microorganisms from four commercial starters suitable for the degradation of domestic wastes. using this technique, three strains of lipase-producing bacteria were isolated and identified: pantoea agglomerans (bb96cc1, bb168cc2) and pseudomonas fluorescens (bw96cc1). in addition, butter fat induced more lipase production when present in the growth medium. interesterification of butter fat triacylglyce ...19968640605
binding between lipopolysaccharide and cecropin a.cecropin a (ca), a bioactive peptide, produced significant lethality to pantoea agglomerans (pa) at low concentrations. significant mortality occurred immediately after addition of ca. separate preincubations of lipopolysaccharides (lps) from the following bacteria: pa, serratia marcescens, escherichia coli (ec), and salmonella typhimurium with ca were performed prior to the bioassay. ca was also preincubated with diphosphoryl lipid a (dpl-a) from ec and s. minnesota (sm), trilinolein, palmitic, ...19958569759
the tonb-dependent ferrichrome receptor fcua of yersinia enterocolitica: evidence against a strict co-evolution of receptor structure and substrate specificity.a yersinia enterocolitica receptor mutant was isolated which is impaired in ferrichrome uptake. the receptor-encoding gene fcua was cloned in escherichia coli k-12. a fcua mutant of y. enterocolitica could be complemented by the cloned dna fragment. the fcua-encoding region was sequenced and an open reading frame encoding 758 amino acids including a signal sequence of 36 amino acids was found. fcua shared 34.6% amino acid sequence homology with fata, the anguibactin receptor of vibrio anguillaru ...19938384682
structure of the nifq gene from enterobacter agglomerans 333 and its overexpression in escherichia coli.the nifq gene, involved in early stages of iron-molybdenum cofactor (femo-co) biosynthesis, was identified downstream of the nifb and niff genes of enterobacter agglomerans. this gene was cloned and its nucleotide sequence determined. the amino acid sequence, as deduced from the nucleotide sequence, revealed an accumulation of cysteine amino acid residues at the c-terminal end of the protein. the cysteine cluster showed the following consensus sequence cys-x4-cys-x2-cys-x5-cys, which is a typica ...19938316214
effects of a lipopolysaccharide from pantoea agglomerans on the cocaine-induced place preference.a lipopolysaccharide from pantoea agglomerans (lpsp) was purified, and its effect on the cocaine-induced place preference was examined in rats. cocaine (4 mg/kg, i.p.) produced a significant place preference. administration of lpsp (5-1000 micrograms/kg, i.p.) alone resulted in neither preference nor aversion for either the drug- or saline-associated place. however, pretreatment with lpsp (500 and 1000 micrograms/kg, i.p.) abolished the place preference that had been induced by cocaine. furtherm ...19948295482
antinociceptive effect of lipopolysaccharide from pantoea agglomerans on streptozotocin-induced diabetic mice.the antinociceptive effect of lipopolysaccharide from pantoea agglomerans (lpsp) in streptozotocin-induced diabetic mice was examined. although subcutaneous (s.c.) administration of lpsp produced a dose-dependent inhibition of the tail-flick response in both non-diabetic and diabetic mice, the antinociceptive response was greater in diabetic mice than in non-diabetic mice. the antinociceptive effects of lpsp in both diabetic and non-diabetic mice were significantly antagonized by s.c. administra ...19948137875
fur regulon in gram-negative bacteria. identification and characterization of new iron-regulated escherichia coli genes by a fur titration assay.a highly sensitive genetic screen for the detection of cloned genes coding for iron-regulated and iron-storage/binding proteins was developed. the fur titration assay (furta) enabled identification of cloned iron-regulated genes from different gram-positive and gram-negative bacteria such as: bacillus subtilis, escherichia coli, pantoea agglomerans, pseudomonas putida, salmonella typhimurium, serratia marcescens and yersinia enterocolitica. an ordered e. coli cosmid library was screened for eith ...19948107138
dual regulation of inaa by the multiple antibiotic resistance (mar) and superoxide (soxrs) stress response systems of escherichia coli.the roles of the marrab (multiple antibiotic resistance) operon and soxrs (superoxide response) genes in the regulation of inaa, an unlinked weak-acid-inducible gene, were studied. inaa expression was estimated from the beta-galactosidase activity of a chromosomal inaa1::lacz transcriptional fusion. marr mutations that elevate marrab transcription and engender multiple antibiotic resistance elevated inaa expression by 10- to 20-fold over that of the wild-type. similarly, one class of inaa consti ...19947928997
suppression of hyperalgesia in streptozotocin-induced diabetic mice by a lipopolysaccharide from pantoea agglomerans.the ability of a lipopolysaccharide from pantoea agglomerans (lpsp) to relieve hyperalgesia was examined by observing its inhibition of the decrease in the threshold for nociceptive perception, as determined by the tail-pinch test, in streptozotocin-induced diabetic mice. subcutaneous injection of lpsp suppressed hyperalgesia in streptozotocin-induced diabetic mice and also exerted a therapeutic effect on hyperalgesia in these animals. the present data suggest that lpsp may be effective in relie ...19947920422
cloning and sequencing of an ice nucleation active gene of erwinia uredovora.an ice nucleation activity gene, named inau, of the bacterium erwinia uredovora kuin-3 has been sequenced. this gene encodes a protein of 1034 amino acid residues, and its expression product, inau protein, has an 832-amino acid residue segment consisting of 52 repeats of closely related 16-amino acid motifs (r-domain), flanked by n- and c-terminal sequences (n- and c-domains, respectively). the primary structure of the inau protein is similar to those of the inaa, inaw, and inaz gene products of ...19947764866
demonstration of the indolepyruvate decarboxylase gene homologue in different auxin-producing species of the enterobacteriaceae.different enterobacteriaceae were assayed for their ability to produce the plant hormone indole-3-acetate with the aim to study the distribution of the indole-3-pyruvate pathway, which is known to be involved in the production of indole-3-acetate in a root-associated enterobacter cloacae strain. other e. cloacae strains, and also enterobacter agglomerans strains, pantoea agglomerans, klebsiella aerogenes, and klebsiella oxytoca were found to convert tryptophan into indole-3-acetate. as it was al ...19947704833
[immunomodulator as medicine for morphine and cocaine dependence. especially effect of lps].the majority of medicines developed for narcotic and stimulant dependence were agonists or antagonists of neurotransmitters and their receptors. recently it has been shown that the nervous system, the endocrine system and the immune system are regulated by mutual interaction. the nervous system and the endocrine system are influenced by an immunomodulator, and the immune system is regulated by a neurotransmitter. in this report, medicines for morphine and cocaine dependence are first surveyed an ...19957699580
capsular polysaccharide biosynthesis and pathogenicity in erwinia stewartii require induction by an n-acylhomoserine lactone autoinducer.n-acylhomoserine lactone (acyl-hsl)-mediated gene expression, also called autoinduction, is conserved among diverse gram-negative bacteria. in the paradigm vibrio fischeri system, bioluminescence is autoinducible, and the lux operon requires the transcriptional activator luxr and the acyl-hsl autoinducer for expression. the production of the acyl-hsl signal molecule is conferred by the luxi gene, and luxr encodes the transcriptional regulator. we show here that erwinia stewartii, the etiological ...19957665477
[a case report of far advanced gastric cancer with multiple liver metastasis (h3) treated with transarterial intermittent chemotherapy and intradermal administration of low molecular lipopolysaccharide (lpsp) extracted from pantoea agglomerans].a case of far advanced gastric cancer with multiple liver metastasis (h3) was treated with transarterial intermittent chemotherapy (5-fu: 250 mg/week, farmorubicin: 10 mg/4 weeks, mmc: 4 mg/2 weeks) and intradermal administration of low molecular lipopolysaccharide (lpsp) extracted from pantoea agglomerans. the ct examination and endoscopy showed regression of the tumor and the patient was discharged from the hospital. lpsp was given at the concentration of 0.1 microgram initially, and the dose ...19957574794
an ice nucleation active gene of erwinia ananas. sequence similarity to those of pseudomonas species and regions required for ice nucleation activity.the ice nucleation active gene, inaa, of erwinia ananas in-10 has been sequenced. this gene encodes a protein composed of 1322 amino acid residues. the inaa protein contains a 1120-residue segment consisting of 70 repeats of closely related 16 amino acid motifs (r-domain), which is flanked by n- and c-terminal sequences (n- and c-domains, respectively). its primary structure is similar to, but not identical with, those of pseudomonas inaw and inaz gene products. by truncating the inaa gene to va ...19892599095
erwinia persicinus, a new species isolated from plants.five strains of a gram-negative, oxidase-negative, facultatively anaerobic, fermentative, motile, rod-shaped bacterium with the general characteristics of the family enterobacteriaceae were isolated from tomatoes (three strains), a banana, and a cucumber. all of the strains produced a water-soluble pink pigment. as determined by dna hybridization (hydroxyapatite method) these five strains were 85 to 100% related in 60 and 75 degrees c reactions, and related sequences exhibited 1% or less base se ...19902275853
phenotypical properties of enterobacter agglomerans (pantoea agglomerans) from human, animal and plant sources.clinical, animal and plant isolates, representing different geographical areas, were identified as enterobacter agglomerans (pantoea agglomerans) using a quantitative bacterial dot method for dna-dna hybridization. the phenotypical properties of the 65 strains were investigated by conventional test methods. no strain decarboxylated ornithine. twenty-two strains, mainly plant isolates, showed delayed acid production from alpha-methyl-glycoside, a trait which may have ecological significance. with ...19912036218
transport and utilization of ferrioxamine-e-bound iron in erwinia herbicola (pantoea agglomerans).we have analyzed ferrioxamine-e-mediated iron uptake and metabolization in erwinia herbicola k4 (pantoea agglomerans) by means of in vivo mössbauer spectroscopy and radioactive labeling techniques. a comparison of cell spectra with the spectrum of ferrioxamine clearly demonstrates that ferrioxamine e is not accumulated in the cell, indicating a fast metal transfer. only two major components of iron metabolism can be detected, a ferric and a ferrous species. at 30 min after uptake, 86% of the int ...19911931438
the specificity of bacterial siderophore receptors probed by bioassays.the ability to utilize siderophores of bacterial and fungal origin has been studied in wild-type and mutant strains of the enterobacterial genera salmonella, escherichia, shigella, moellerella, klebsiella, enterobacter, hafnia, pantoea, ewingella, tatumella, yersinia, and in the non-enterics aeromonas, pseudomonas and aureobacterium. although only a few representative strains were tested, the results show characteristic genus-specific differences in the utilization of hydroxamate and catecholate ...19911663779
chiral linear hydroxamates as biomimetic analogues of ferrioxamine and coprogen and their use in probing siderophore-receptor specificity in bacteria and fungi.linear hydroxamate derivatives, possessing chiral alpha-amino acid moieties, were synthesized and their iron transport activities were studied in bacteria and fungi. no growth-promoting activity could be detected in the gram-positive hydroxamate-auxotroph aureobacterium flavescens jg9. however, gram-negative enterobacteria, such as escherichia coli, pantoea agglomerans and hafnia alvei were able to utilize iron from these analogues. uptake of 55fe-labeled analogues was inhibited by sodium azide, ...19911657086
pantoea punctata sp. nov., pantoea citrea sp. nov., and pantoea terrea sp. nov. isolated from fruit and soil samples.a total of 37 bacterial strains with the general characteristics of the family enterobacteriaceae were isolated from fruit and soil samples in japan as producers of 2,5-diketo-d-gluconic acid from d-glucose. these organisms were phenotypically most closely related to the genus pantoea (f. gavini, j. mergaert, a. beji, c. mielearek, d. izard, k. kersters, and j. de ley, int. j. syst. bacteriol. 39:337-345, 1989) and were divided into three phenotypic groups. we selected nine representative strain ...19921581180
ph dependence and gene structure of inaa in escherichia coli.the weak-acid-inducible locus inaa in escherichia coli was mapped to 48.6 min by p1 cotransduction of inaa mud lac fusions and linked tn10 insertions. the inaa1::lac fusion tested negative for phenotypes characteristic of mutations in the nearby locus ubig. sequence analysis of a fragment amplified by polymerase chain reaction located the inaa1::lac fusion joint within an open reading frame 311 nucleotides downstream of nrdb, transcribed in the opposite direction, encoding a 168-amino-acid polyp ...19921537798
homeostasis as regulated by activated macrophage. iii. protective effect of lpsw (lipopolysaccharide (lps) of wheat flour) on gastric ulcer in mice as compared with those of other lps from various sources.protective effect of lipopolysaccharide (lps) from various sources on gastric ulcer has been examined in mice using parenteral as well as oral route. ulcer is induced by indomethacin, stress or alcohol. lps was prepared from 6 species of bacteria (escherichia coli, pantoea agglomerans, serratia ficaria, enterobacter cloacae, bordetella pertussis, alcaligenes faecalis) and from wheat flour. when administered intravenously, lps of pantoea agglomerans was the most effective among other lps examined ...19921525959
homeostasis as regulated by activated macrophage. ii. lps of plant origin other than wheat flour and their concomitant bacteria.in order to seek a macrophage-activating substance, lipopolysaccharide (lps) of plant origin other than that of wheat flour was surveyed. a large amount of lps (10-100 micrograms/g) was detected in laminaria japonica (kelp), curcuma longa (turmeric), undaria pinnatifida and other substances. since concomitant bacteria possibly existing in root of farm products can be considered to contribute to lps of plant origin, a count was taken of bacterial cells both dead and alive. this count revealed tha ...19921525958
inhibition of morphine dependence by a lipopolysaccharide from pantoea agglomerans.a lipopolysaccharide from pantoea agglomerans (lpsp) was purified and examined for relief of morphine dependence by observing its inhibition of the jumping of mice on naloxone-precipitate withdrawal. administration of lpsp either intravenously or intradermally showed marked inhibition of the jumping. beta-endorphin in mouse serum and brain tissue were recognized to be in synchrony with the time course of the relief. administration of tnf-alpha gave similar effect, suggesting that lpsp induces a ...19921421014
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