Publications

TitleAbstractYear
Filter
PMID(sorted descending)
Filter
survival of lung transplant patients with cystic fibrosis harboring panresistant bacteria other than burkholderia cepacia, compared with patients harboring sensitive bacteria.the impact of panresistant bacteria, other than burkholderia cepacia, on the survival after lung transplantation in patients with cystic fibrosis (cf) remains controversial.200717692788
antibiotic deactivation by a dizinc beta-lactamase: mechanistic insights from qm/mm and dft studies.hybrid quantum mechanical/molecular mechanical (qm/mm) methods and density functional theory (dft) were used to investigate the initial ring-opening step in the hydrolysis of moxalactam catalyzed by the dizinc l1 beta-lactamase from stenotrophomonas maltophilia. anchored at the enzyme active site via direct metal binding as suggested by a recent x-ray structure of an enzyme-product complex (spencer, j.; et al. j. am. chem. soc. 2005, 127, 14439), the substrate is well aligned with the nucleophil ...200717691780
endophytic bacterial diversity in rice (oryza sativa l.) roots estimated by 16s rdna sequence analysis.the endophytic bacterial diversity in the roots of rice (oryza sativa l.) growing in the agricultural experimental station in hebei province, china was analyzed by 16s rdna cloning, amplified ribosomal dna restriction analysis (ardra), and sequence homology comparison. to effectively exclude the interference of chloroplast dna and mitochondrial dna of rice, a pair of bacterial pcr primers (799f-1492r) was selected to specifically amplify bacterial 16s rdna sequences directly from rice root tissu ...200817690836
isolation of maize soil and rhizosphere bacteria with antagonistic activity against aspergillus flavus and fusarium verticillioides.bacterial isolates from mississippi maize field soil and maize rhizosphere samples were evaluated for their potential as biological control agents against aspergillus flavus and fusarium verticillioides. isolated strains were screened for antagonistic activities in liquid coculture against a. flavus and on agar media against a. flavus and f. verticillioides. we identified 221 strains that inhibited growth of both fungi. these bacteria were further differentiated by their production of extracellu ...200717685333
life-threatening septic shock in a pregnant woman with ileus.septic shock during pregnancy is very rare but has high mortality. we report a case of septic shock in a pregnant woman with ileus, showing that severe ileus in a pregnant woman could be attributed to life-threatening septic shock and that we should give special attention to a nosocomial infection.200717674261
in vitro efficacy of copper and silver ions in eradicating pseudomonas aeruginosa, stenotrophomonas maltophilia and acinetobacter baumannii: implications for on-site disinfection for hospital infection control.pseudomonas aeruginosa, stenotrophomonas maltophilia and acinetobacter baumannii are major opportunistic waterborne pathogens causing hospital-acquired infections. copper-silver ionization has been shown to be effective in controlling legionella colonization in hospital water systems. the objective was to determine the efficacy of copper and silver ions alone and in combination in eradicating p. aeruginosa, s. maltophilia and a. baumannii at the concentration applied to legionella control. kill ...200817655912
bacterial contamination of water in dental unit reservoirs.the aim of this study was bacteriological assessment of water in dental unit reservoirs--concentration and composition of the aerobe and facultative anaerobe bacterial microflora. reservoir water samples were taken from 25 units. bacterial flora were determined with the plate culture method. bacteria were identified with biochemical microtests: api 20e, api 20ne (biomérieux, france) and gp2 microplatetm (biolog, usa). the concentration of total bacteria isolated from one site was 201,039 cfu/ml, ...200717655191
[the present status, counter-measures and new trends on burn infection].in recent fifty years, pseudomonas aeruginosa and staphylococcus aureus were continuously the predominant in burn infections, the only change seen was a rapid increase in their drug-resistance. under the pressure of antibiotics, some opportunistic bacteria that were resistant to all available antibiotics emerged, such as acinetobacter baumanii and maltophilia stenotrophomonas. for critically burn patients, basing on early surgical intervention, early and short-term use of broad-spectrum antibiot ...200717649876
ventilator-associated pneumonia in the home care setting.to describe the rate of infection, associated organisms, and potential risk factors for ventilator-associated pneumonia (vap) in patients receiving mechanical ventilation at home.200717620236
high-throughput determination of biochemical oxygen demand (bod) by a microplate-based biosensor.the use of the conventional 5-day biochemical oxygen demand (bod5) method in bod determination is greatly hampered by its time-consuming sampling procedure and its technical difficulty in the handling of a large pool of wastewater samples. thus, it is highly desirable to develop a fast and high-throughput biosensor for bod measurements. this paper describes the construction of a microplate-based biosensor consisting of an organically modified silica (ormosil) oxygen sensing film for high-through ...200717612187
cultivable bacterial diversity of alkaline lonar lake, india.aerobic, alkaliphilic bacteria were isolated and characterized from water and sediment samples collected in the winter season, january 2002 from alkaline lonar lake, india, having ph 10.5. the total number of microorganisms in the sediment and water samples was found to be 10(2)-10(6) cfu g(-1) and 10(2)-10(4) cfu ml(-1), respectively. one hundred and ninety-six strains were isolated using different enrichment media. to study the bacterial diversity of lonar lake and to select the bacterial stra ...200817604989
rhizobacterial volatiles affect the growth of fungi and arabidopsis thaliana.volatiles of stenotrophomonas, serratia, and bacillus species inhibited mycelial growth of many fungi and arabidopsis thaliana (40 to 98%), and volatiles of pseudomonas species and burkholderia cepacia retarded the growth to lesser extents. aspergillus niger and fusarium species were resistant, and b. cepacia and staphylococcus epidermidis promoted the growth of rhizoctonia solani and a. thaliana. bacterial volatiles provide a new source of compounds with antibiotic and growth-promoting features ...200717601806
convenient test using a combination of chelating agents for detection of metallo-beta-lactamases in the clinical laboratory.although transmissible metallo-beta-lactamases (mbls) are a serious threat to beta-lactam antibiotic therapy, the clsi currently does not recommend testing methods for the detection of mbls. the aim of this study was to evaluate the capability of double-disk tests (ddts) by using disks containing a combination of the chelators 2-mercaptopropionic acid (mpa) and tris-edta (te) to detect mbls. sixteen isolates (4 acinetobacter baumannii isolates, 6 pseudomonas aeruginosa isolates, 1 serratia marce ...200717596358
atrazine degradation by aerobic microorganisms isolated from the rhizosphere of sweet flag (acorus calamus l.).in presented study the capability of microorganisms isolated from the rhizosphere of sweet flag (acorus calamus) to the atrazine degradation was assessed. following isolation of the microorganisms counts of psychrophilic bacteria, mesophilic bacteria and fungi were determined. isolated microorganisms were screened in terms of their ability to decompose a triazine herbicide, atrazine. our results demonstrate that within the rhizosphere of sweet flag there were 3.8x10(7) cfu of psychrophilic bacte ...200817594524
[clinical distribution and antibiotic resistance of non-fermentative gram-negative bacilli infection after liver transplantation].to investigate the epidemiology of non-fermentative gram-negative bacilli infections after liver transplantation, and to analyze the antibiotic susceptibility to major pathogens.200717593853
isotopic analysis of n and o in nitrite and nitrate by sequential selective bacterial reduction to n2o.nitrite is an important intermediate species in the biogeochemical cycling of nitrogen, but its role in natural aquatic systems is poorly understood. isotopic data can be used to study the sources and transformations of no2- in the environment, but methods for independent isotopic analyses of no2- in the presence of other n species are still new and evolving. this study demonstrates that isotopic analyses of n and o in no2- can be done by treating whole freshwater or saltwater samples with the d ...200717580983
a cyclic amp receptor protein-regulated cell-cell communication system mediates expression of a feca homologue in stenotrophomonas maltophilia.stenotrophomonas maltophilia wr-c possesses an rpf/diffusible signal factor (dsf) cell-cell communication system. it produces cis-delta2-11-methyl-dodecenoic acid, a dsf, and seven structural derivatives, which require rpff and rpfb for synthesis. acquisition of iron from the environment is important for bacterial growth as well as the expression of virulence genes. we identified a gene homologous to feca, which encodes a ferric citrate receptor that transports exogenous siderophore ferric citra ...200717574998
successful engraftment of the second reduced-intensity conditioning cord blood transplantation (cbt) for a patient who developed graft rejection and infectious complications after the first cbt for aml. 200717572713
effect of environmental factors on biofilm formation by clinical stenotrophomonas maltophilia isolates.the influence of environmental factors (temperature, aerobiosis-anaerobiosis, static-dynamic conditions, ph) was determined on biofilm formation by 51 s. maltophilia clinical isolates. the strains produced more biofilm at 32 degrees c than at 37 or 18 degrees c. aerobic and 6% co2 atmosphere yielded comparable biofilm amounts, higher than under anaerobic conditions. biofilm production was not affected by static vs. agitated culture conditions. biofilm production at ph 7.5 and 8.5 was comparable ...200717571802
fatal pulmonary hemorrhage in a patient with myelodysplastic syndrome and fulminant pneumonia caused by stenotrophomonas maltophilia. 200717565466
microbiology of ventilator-associated pneumonia compared with that of hospital-acquired pneumonia.nosocomial pneumonia is the leading cause of mortality attributed to nosocomial infection. appropriate empirical therapy has been associated with improved survival, but data are limited regarding the etiologic agents of hospital-acquired pneumonia in nonventilated patients (hap). this evaluation assessed whether the currently recommended empirical therapy is appropriate for both ventilator-associated pneumonia (vap) and hap by evaluating the infecting flora.200717564985
emerging and unusual gram-negative infections in cystic fibrosis.people with cystic fibrosis (cf) have chronic airway infection and frequent exposure to antibiotics, which often leads to the emergence of resistant organisms. in addition to the development of multiresistance in common cf pathogens such as pseudomonas aeruginosa, several newer, inherently resistant gram-negative species are becoming more common, including burkholderia cepacia complex, stenotrophomonas maltophilia, achromobacter (alcaligenes) xylosoxidans, certain ralstonia species, and those wi ...200717562501
global emergence of trimethoprim/sulfamethoxazole resistance in stenotrophomonas maltophilia mediated by acquisition of sul genes.trimethoprim/sulfamethoxazole (tmp/smx) resistance remains a serious threat in the treatment of stenotrophomonas maltophilia infections. we analyzed an international collection of 55 s. maltophilia tmp/smx-sensitive (s) (n=30) and -resistant (r) (n=25) strains for integrons; sul1, sul2 and dhfr genes; and insertion element common region (iscr) elements. sul1, as part of a class 1 integron, was detected in 17 of 25 tmp/smx-r. nine tmp/smx-r strains carried sul2; 7 were on large plasmids. five tmp ...200717553270
characterization of symbiotic and endophytic bacteria isolated from root nodules of herbaceous legumes grown in qinghai-tibet plateau and in other zones of china.qinghai-tibet plateau is the highest place in the world and the environment in that plateau is hard for animals and plants, with low temperature, low concentration of oxygen and high solar radiation. in this study, 61 root nodule isolates from vicia, oxytropis, medicago, melilotus and onobrychis species grown in qinghai-tibet plateau and in loess plateau were comparatively characterized. based upon the results of numerical taxonomy, ardra, aflp, dna-dna hybridization and 16s rdna sequencing, the ...200717541555
bloodstream infection caused by stenotrophomonas maltophilia in children.stenotrophomonas maltophilia, a multidrug resistant gram-negative pathogen, has become a more frequent cause of bloodstream infections (bsi). little is known about development of s. maltophilia bacteremia in children. the objective of this study was to define risk factors and outcomes associated with s. maltophilia bsi in children.200717529868
genotype and antibiotic susceptibility patterns of pseudomonas aeruginosa and stenotrophomonas maltophilia isolated from cystic fibrosis patients.the purpose of this study was to type the pseudomonas aeruginosa and stenotrophomonas maltophilia isolates recovered from cystic fibrosis (cf) patients by random amplified polymorphic dna (rapd)-pcr and to determine the antibiotic susceptibility of these strains. p. aeruginosa (n=49), and s. maltophilia (n=11) isolates which had been recovered from 16 and 8 patients, respectively, during a 1-year period were investigated. three primers were used for rapd-pcr typing. antibiotic susceptibility tes ...200717515637
composting and vermicomposting experiences in the treatment and bioconversion of asphaltens from the prestige oil spill.this work illustrates the effectiveness of composting and vermicomposting in degrading fuel-in-water emulsions from oil spills (chapapote), and the isolation of potentially useful microorganisms for its biodegradation. firstly, an alternative to the biodegradation of asphaltens from the prestige oil spill (still present in some chapapote rafts in the cantabrian coast) by means of the application of composting techniques to a microbial partnership acclimated to fuel-oil is offered. our aim is tha ...200817512195
evaluation of the colorimetric vitek 2 card for identification of gram-negative nonfermentative rods: comparison to 16s rrna gene sequencing.ninety strains of a collection of well-identified clinical isolates of gram-negative nonfermentative rods collected over a period of 5 years were evaluated using the new colorimetric vitek 2 card. the vitek 2 colorimetric system identified 53 (59%) of the isolates to the species level and 9 (10%) to the genus level; 28 (31%) isolates were misidentified. an algorithm combining the colorimetric vitek 2 card and 16s rrna gene sequencing for adequate identification of gram-negative nonfermentative r ...200717507509
[mesenteric vein thrombosis and protein c and s deficiency in a patient with chronic hepatitis c on treatment with interferon and ribavirin].we describe the case of a 67 year old male with chronic hepatitis c on treatment with pegylated interferon and ribavirin who, after two and a half months of combined treatment, presented with a picture of acute mesenteric vein thrombosis that required urgent surgery. it re-occurred several days later and was his cause of death. in the thrombophilia study carried out immediately after surgery a decrease in protein c and s was considered as a cause of hypercoagulability. protein c and s deficiency ...200717493436
[primary intracerebral hemorrhage due to probable cerebral amyloid angiopathy complicated by brain abscess: case report].cerebral amyloid angiopathy (caa), in which deposition of amyloid within the arterial media and adventitia is the remarkable feature, causes spontaneous lober cerebral hemorrhage in elderly person, and some reports show the quite high occurrence rate of this entity among intracerebral hemorrhage in patents above 70 years old. brain abscess resulting from intracerebral hemorrhage is rare. to our knowledge, no report of such hemorrhage which is caused by caa has been published so far. we report a ...200717491345
comparative review of the carbapenems.the carbapenems are beta-lactam antimicrobial agents with an exceptionally broad spectrum of activity. older carbapenems, such as imipenem, were often susceptible to degradation by the enzyme dehydropeptidase-1 (dhp-1) located in renal tubules and required co-administration with a dhp-1 inhibitor such as cilastatin. later additions to the class such as meropenem, ertapenem and doripenem demonstrated increased stability to dhp-1 and are administered without a dhp-1 inhibitor. like all beta-lactam ...200717488146
thirteen-lined ground squirrels (spermophilus tridecemlineatus) harbor multiantibiotic-resistant bacteria.whether wild-caught animals used for biomedical research carry antibiotic-resistant bacteria is not well studied. thirteen- lined ground squirrels (spermophilus tridecemlineatus) are small mammals used to study hibernation. these animals are captured from the wild or are born in laboratory animal facilities to wild-caught mothers. because microorganisms harbored by 13-lined ground squirrels may be pathogenic to their caretakers and other laboratory animals, learning more about antibiotic resista ...200717487947
risk factors for infections with multidrug-resistant stenotrophomonas maltophilia in patients with cancer.stenotrophomonas maltophilia is responsible for an increasing number of infections, especially in hospitalized patients. therapy options are limited and trimethoprim/sulfamethoxazole (tmp/smx) is often the main treatment option for this infection. in the current study, the risk factors were determined for the emergence of multidrug-resistant (mdr) s. maltophilia.200717487860
conjugative transfer of preferential utilization of aromatic compounds from pseudomonas putida csv86.pseudomonas putida csv86 utilizes naphthalene (nap), salicylate (sal), benzyl alcohol (balc), and methylnaphthalene (mn) preferentially over glucose. methylnaphthalene is metabolized by ring-hydroxylation as well as side-chain hydroxylation pathway. although the degradation property was found to be stable, the frequency of obtaining nap(-)sal(-)mn(-)balc(-) phenotype increased to 11% in the presence of curing agents. this property was transferred by conjugation to stenotrophomonas maltophilia cs ...200817487554
clinical problem-solving. the drenched doctor - a 55-year-old male physician was seen in august because of a 1-week history of fever and night sweats. 200717476014
disseminated cutaneous fusarium moniliforme infections in a leukemic child.a 5-year-old boy had a 10-month remission of acute lymphocytic leukemia (all) after chemotherapy. re-induction chemotherapy was performed for relapse of all. thereafter, he suffered from an episode of neutropenic fever with pneumonia. one week following control of the condition with antibiotics, a 1 x 1-cm, red, painful nodule appeared on the left thigh, which was initially suspected to be pseudomonas infection. parenteral ceftazidime and amikacin were administered, but persistent high fever, mi ...200717472677
diffusible signal factor-dependent cell-cell signaling and virulence in the nosocomial pathogen stenotrophomonas maltophilia.the genome of stenotrophomonas maltophilia encodes a cell-cell signaling system that is highly related to the diffusible signal factor (dsf)-dependent system of the phytopathogen xanthomonas campestris. here we show that in s. maltophilia, dsf signaling controls factors contributing to the virulence and antibiotic resistance of this important nosocomial pathogen.200717468254
selection for biocontrol bacteria antagonistic toward rosellinia necatrix by enrichment of competitive avocado root tip colonizers.biological control of soil-borne pathogens is frequently based on the application of antagonistic microorganisms selected solely for their ability to produce in vitro antifungal factors. the aim of this work was to select bacteria that efficiently colonize the roots of avocado plants and display antagonism towards rosellinia necatrix, the causal agent of avocado white root rot. a high frequency of antagonistic strains (ten isolates, 24.4%) was obtained using a novel procedure based on the select ...200717467245
successfully treated nosocomial stenotrophomonas maltophilia bacteremia following desensitization to trimethoprim-sulfamethoxazole.stenotrophomonas maltophilia has emerged as an important cause of morbidity and mortality in hospitalized patients. because trimethoprim-sulfamethoxazole (tmp-smx) remains the most effective drug for the treatment of s. maltophilia infections, desensitization should be considered in patients with hypersensitivity to tmp-smx.200717458682
stenotrophomonas maltophilia infection of the central venous catheter and lysis of an old thrombosis in a post-bone marrow transplantation child: implications in the fibrinolytic process.in this report, we describe an 11-y-old girl who developed jugular venous thrombosis after allogeneic bone marrow transplantation for lymphoma and then experienced dissolution of the thrombosis following catheter-related stenothrophomonas maltophilia bactearemia. the lysis of the old thrombosis around the central venous catheter suggested a local fibrinolytic activity of s. maltophilia. the global fibrinolytic capacity (gfc) was also tested in vitro by using s. maltophilia cultures obtained from ...200717454909
isolation and characterization of diuron-degrading bacteria from lotic surface water.the bacterial community structure of a diuron-degrading enrichment culture from lotic surface water samples was analyzed and the diuron-degrading strains were selected using a series of techniques combining temporal temperature gradient gel electrophoresis (ttge) of 16 s rdna gene v1-v3 variable regions, isolation of strains on agar plates, colony hybridization methods, and biodegradation assays. the ttge fingerprints revealed that diuron had a strong impact on bacterial community structure and ...200717450392
surface charge influences enterococcal prevalence in mixed-species biofilms.to study the influence of 15 microbial isolates on the prevalence of charge-heterogeneous and charge-homogeneous enterococcus faecalis strains, all isolated from biliary stents, in mixed-species biofilms.200717448160
removal of cadmium ions during stationary growth phase by an extremely cadmium-resistant strain of stenotrophomonas sp.stenotrophomonas sp. cd02 was isolated from a site that previously had been contaminated with high concentrations of the heavy metals cadmium (3 mg kg(-1)) and chromium (115 mg kg(-1)). this strain was able to grow on complex (luria bertani) medium containing high concentrations of cadmium ion (up to 4 mm). additionally, it could remove up to 80% of the dissolved ions but only after reaching stationary growth phase. strain cd02 also tolerated high concentrations of other heavy metals such as chr ...200717447550
the efficacy of nonsurgical treatment of infected pancreatic necrosis.we conducted this study to evaluate the efficacy of nonsurgical treatment for patients with infected pancreatic necrosis (ipn).200717446837
potential of the adhesion of bacteria isolated from drinking water to materials.heterotrophic bacteria (11 genera, 14 species, 25 putative strains) were isolated from drinking water, identified either biochemically or by partial 16s rdna gene sequencing and their adherence characteristics were determined by two methods: i. thermodynamic prediction of adhesion potential by measuring hydrophobicity (contact angle measurements) and ii. by measuring adherence to eight different substrata (asi 304 and 316 stainless steel, copper, polyvinyl chloride, polypropylene, polyethylene, ...200717440920
characterization of a novel t4-type stenotrophomonas maltophilia virulent phage smp14.stenotrophomonas maltophilia (sm), with most of the isolates being resistant to multidrugs, is an opportunistic bacterium causing nosocomial infections. in this study, a novel virulent sm phage, smp14, was characterized. electron microscopy showed that smp14 resembled members of myoviridae and adsorbed to poles of the host cells during infection. it lysed 37 of 87 clinical sm isolates in spot test, displayed a latent period of ca. 20 min, and had a burst size of ca. 150. its genome (estimated to ...200717440710
[study on the structure and function of a stable methane-oxidizing mixed microbial consortium].from an agricultural sample taken in chongqing, a stable methane-oxidizing mixed microbial consortium was established by enrichment culture with methane as a sole source of carbon and energy. the mixed consortium showed high capability of phenol degradation and 1,2-epoxypropane production from propene. more than 99% of phenol at an initial concentration of 600mg/l could be degraded by the mixed microbial consortium after 11 h of cultivation. the productivity of 1, 2-epoxypropane could be increas ...200717436634
relationship between immunosuppression and intensive care unit-acquired multidrug-resistant bacteria: a case-control study.to determine the relationship between immunosuppression and intensive care unit (icu)-acquired multidrug-resistant (mdr) bacteria.200717414081
bacterial reduction of selenium in coal mine tailings pond sediment.sediment from a storage facility for coal tailings solids was assessed for its capacity to reduce selenium (se) by native bacterial community. one se(6+)-reducing bacterium enterobacter hormaechei (tar11) and four se(4+)-reducing bacteria, klebsiella pneumoniae (tar1), pseudomonas fluorescens (tar3), stenotrophomonas maltophilia (tar6), and enterobacter amnigenus (tar8) were isolated from the sediment. enterobacter hormaechei removed 96% of the added se(6+) (0.92 mg l(-1)) from the effluents whe ...200717412898
faropenem: review of a new oral penem.faropenem medoxomil is a new orally administered penem antibiotic. its chiral tetrahydrofuran substituent at position c2 is responsible for its improved chemical stability and reduced cns effects, compared with imipenem. faropenem demonstrates broad-spectrum in vitro antimicrobial activity against many gram-positive and -negative aerobes and anaerobes, and is resistant to hydrolysis by nearly all beta-lactamases, including extended-spectrum beta-lactamases and ampc beta-lactamases. however, faro ...200717402834
presumed pseudobacteremia outbreak resulting from contamination of proportional disinfectant dispenser.reported here are the microbiological and epidemiological details of a presumed outbreak of aerobic gram-negative bacilli infections affecting 19 hematological patients, which was traced to contaminated disinfectant. over a 5-month period, the following organisms were isolated from the blood cultures of 19 neutropenic patients: pseudomonas fluorescens (n = 13), achromobacter xylosoxidans (n = 12), comamonas testosteroni (n = 2) or stenotrophomonas maltophilia (n = 1). the affected patients were ...200717393202
antibiotic binding to dizinc beta-lactamase l1 from stenotrophomonas maltophilia: scc-dftb/charmm and dft studies.a dizinc beta-lactamase (l1 from stenotrophomonas maltophilia) complexed with an antibiotic compound (moxalactam) has been studied using a hybrid quantum mechanical/molecular mechanical (qm/mm) approach. the qm region is described by the self-consistent charge-density functional tight binding (scc-dftb) model while the mm by charmm. the michaelis complex, which is constructed from a recent x-ray structure of the l1 enzyme with the hydrolyzed moxalactam, is simulated by molecular dynamics. the si ...200717388313
[investigation of the structure of biofilms formed by sulfur cycle bacteria on metal matrices].the structure of biofilm formed by thiobacillus thioparus, stenotrophomonas maltophilia on the metal matrix was investigated. use of electron microscope-microanalysis showed that these bacteria can form biofilms 34-94 microm thick on metal different in their structure and elemental sulfur composition. a biofilm formed by mixed culture of thiobacillus thioparus and stenotrophomonas maltophilia had a developed interior structure and biofilm porosity. the use of the confocal laser scanning microsco ...200617388123
physiological and phylogenetic characterization of a stable benzene-degrading, chlorate-reducing microbial community.a stable anoxic enrichment culture was obtained that degraded benzene with chlorate as an electron acceptor. the benzene degradation rate was 1.65 mm benzene per day, which is similar to reported aerobic benzene degradation rates but 20-1650 times higher than reported for anaerobic benzene degradation. denaturing gradient gel electrophoresis of part of the 16s rrna gene, cloning and sequencing showed that the culture had a stable composition after the seventh transfer. five bacterial clones were ...200717386037
pathogens in early-onset and late-onset intensive care unit-acquired pneumonia.to compare the type of pathogens isolated from patients with early-onset intensive care unit (icu)-acquired pneumonia with those isolated from patients with late-onset icu-acquired pneumonia and to study risk factors for the isolation of pathogens that are potentially resistant to multiple drugs.200717385143
comparison of etest, vitek and agar dilution for susceptibility testing of colistin.in total, 172 isolates of enterobacteriaceae, acinetobacter spp., pseudomonas aeruginosa and stenotrophomonas maltophilia were tested for susceptibility to colistin by agar dilution, etest and the vitek 2 system. isolates with a colistin mic < or =2 mg/l were considered to be susceptible. fifty-four (31%) gram-negative isolates were resistant to colistin. categorical agreement between agar dilution and etest was 87%, and between agar dilution and vitek 2 was 82%. based on the data obtained, the ...200717371537
empirical antimicrobial treatment for chemotherapy-induced febrile neutropenia.febrile neutropenia in immunocompromised hosts is associated with a high mortality. empirical treatment in such cases is instituted to cover the common pathogens. generally, combination antibiotic treatment is used early in the febrile neutropenia phase. recent studies demonstrate that monotherapy with certain beta-lactam antibiotics can be equally effective. glycopeptide antibiotics are used in the absence of an adequate response to the initial antibiotics. empirical antifungal therapy may be g ...200717346939
antimicrobial therapy for stenotrophomonas maltophilia infections.stenotrophomonas maltophilia has emerged as an important nosocomial pathogen capable of causing respiratory, bloodstream, and urinary infections. the treatment of nosocomial infections by s. maltophilia is difficult, as this pathogen shows high levels of intrinsic or acquired resistance to different antimicrobial agents, drastically reducing the antibiotic options available for treatment. intrinsic resistance may be due to reduced outer membrane permeability or to the multidrug efflux pumps. how ...200717334747
increased incidence of class 1 integrons in trimethoprim/sulfamethoxazole-resistant clinical isolates of stenotrophomonas maltophilia. 200717329265
screening of the antibacterial effects of a variety of essential oils on microorganisms responsible for respiratory infections.the aim of this study was to examine the cytotoxicity and the antibacterial effects of a variety of essential oils on major respiratory tract pathogens. the cytotoxicity of 13 essential oils was evaluated on vero cells. the antibacterial activity was evaluated by the kirby bauer paper method, minimum inhibitory and bactericidal concentration against streptococcus pyogenes, agalactiae, pneumoniae and klebsiella pneumoniae, haemophilus influenzae, staphylococcus aureus and stenotrophomonas maltoph ...200717326042
detection of dna from a range of bacterial species in the knee joints of dogs with inflammatory knee arthritis and associated degenerative anterior cruciate ligament rupture.mixtures of bacterial nucleic acids can often be detected in synovial joints affected with arthritis. we investigated the potential role of such mixtures of bacterial nucleic acids in the pathogenesis of arthritis in a naturally occurring canine model. dogs with a common inflammatory knee arthritis in which associated pathological degenerative anterior cruciate ligament (acl) rupture often develops were studied. synovial biopsies were obtained from 43 dogs with the naturally occurring acl ruptur ...200717320342
emerging cystic fibrosis pathogens: incidence and antimicrobial resistance.we examined the frequency of isolation and the antimicrobial resistance of burkholderia cepacia complex, stenotrophomonas maltophilia and achromobacter xylosoxidans in cystic fibrosis patients from 2000 to 2004. strains susceptibility to tobramycin, piperacillin/tazobactam, imipenem, gentamicin, ciprofloxacin and ceftazidime was determined by disc diffusion assay. b. cepacia complex showed a very high resistance also to ciprofloxacin reaching 100% in 2004. s. maltophilia and a. xvylosoxidans sho ...200717319602
isolation and identification of a novel valienamine-producing bacterium.to isolate, identify and assess valienamine production by a soil bacterial isolate from a wheat field in hangzhou, china.200717309634
benzoic acid-degrading bacteria from the intestinal tract of macrotermes michaelseni sjöstedt.the intestinal tracts of termites host a wide variety of microbial symbionts, which have been implicated in degradative processes. in this study, a fungus-cultivating termite, macrotermes michaelseni was found to harbor 2.2 x 10(6) bacterial cells per ml of gut homogenates capable of degrading benzoic acid. two benzoic acid degrading bacteria were isolated from the highest dilution of gut homogenates in oxic media with benzoic acid as the sole carbon source. isolate cbc was related to stenotroph ...200717304624
the impact of multidrug resistance on the outcomes of critically ill patients with gram-negative bacterial pneumonia.multidrug resistance (mdr) in gram-negative bacteria is on the rise, but its effect on patient outcomes is not well established. the outcomes of 129 adult surgical intensive care unit (sicu) patients treated for gram-negative pneumonia were evaluated in relation to demographics, bacterial etiology, and infections due to mdr bacteria (defined as resistant to all agents except for aminoglycosides and polymyxins). the mean (sd) age and acute physiology and chronic health evaluation (apache) ii scor ...200717300905
cystic fibrosis in adults.the aim of this cross-sectional retrospective study was to describe clinical and functional characteristics and the microbiological profile of an adult population with cystic fibrosis. the study was performed at the pulmonary diseases service of the hospital of the medical school of the state university of campinas. the charts of 54 adults (27 males, 27 females) with cystic fibrosis were reviewed. demographic, clinical, and microbiological data were collected. clinical and functional characteris ...200717294339
application of molecular methods for analysing the distribution and diversity of acetic acid bacteria in chilean vineyards.the presence of acetic acid bacteria populations on grape surfaces from several chilean valleys is reported. the bacteria were analysed at both the species and the strain level by molecular methods such as rflp-pcr 16s rrna gene, rflp-pcr its 16s-23s rrna gene regions and arbitrary primed (ap) pcr. our results show that there are limited numbers of species of acetic acid bacteria in the grapes and that there is a need for an enrichment medium before plating to recover the individual colonies. in ...200717289199
enrichment and isolation of endosulfan degrading and detoxifying bacteria.in the present study, degradation of endosulfan by a mixed culture isolated from a pesticide-contaminated soil was studied in batch experiments. after two weeks of incubation, the mixed culture was able to degrade 73% and 81% of alpha and beta endosulfan respectively. endodiol was identified by gc/ms as degradation intermediate. the toxicity studies of endosulfan before and after degradation were carried out using micronucleus assay on human polymorphonuclear cells. the findings suggested that t ...200717289112
stenotrophomonas maltophilia as a part of normal oral bacterial flora in captive snakes and its susceptibility to antibiotics.only little is known about normal oral bacterial flora in captive snakes containing stenotrophomonas maltophilia. this microbe has been reported as a causative agent of numerous infections in reptiles. therefore, the goal of the study was to detect its presence in the mouths of a significant number of healthy captive snakes and determining its susceptibility to antibiotics at 30 and 37 degrees c. the isolates were obtained in 1999-2005 from mouth swabs of 115 snakes of 12 genera and 22 species-m ...200717276020
stenotrophomonas maltophilia pneumonia after bone marrow transplantation: case report with emphasis on the high-resolution ct findings.a 6-year-old female patient who underwent bone marrow transplantation because of fanconi anaemia presented with fever, dyspnoea and cough 17 days after the procedure. the physical examination revealed diffuse crackles. chest radiographs demonstrated diffuse alveolar opacities in both lungs. high-resolution ct showed a diffuse and bilateral lung lesion characterized by multifocal areas of air-space consolidation associated with ground-glass attenuation and small centrilobular nodules. the culture ...200717267464
identification of diazotrophs in the culturable bacterial community associated with roots of lasiurus sindicus, a perennial grass of thar desert, india.lasiurus sindicus is a highly nutritive, drought-tolerant, perennial grass that is endemic to the thar desert of rajasthan, india. analysis of 16s rrna coding genes of the bacterial isolates enriched in nitrogen-free semisolid medium, from the surface-sterilized roots of l. sindicus, showed predominance of gram-negative over gram-positive bacteria. according to comparative sequence analysis of 16s rdna sequence data, gram-positive bacteria with low gc content (staphylococcus warneri and bacillus ...200717264993
an acquired efflux system is responsible for copper resistance in xanthomonas strain ig-8 isolated from china.the genus xanthomonas contains plant pathogens exhibiting innate resistance to a range of antimicrobial agents. in other genera, multidrug resistance is mediated by a synergy between a low-permeability outer membrane and expression of a number of multidrug efflux systems. this report describes the isolation of a novel gene cluster xmersa from xanthomonas strain ig-8 that mediates copper chloride resistance. subsequent analysis of these genes showed that they were responsible for the high level o ...200717263848
munchausen syndrome by proxy and recurrent polymicrobial bacteremia. 200717259892
stability of a promiscuous plasmid in different hosts: no guarantee for a long-term relationship.broad-host-range (bhr) incp-1 plasmids have the ability to transfer between and replicate in nearly all species of the alpha-, beta- and gammaproteobacteria, but surprisingly few data are available on the stability of these plasmids in strains within their host range. moreover, even though molecular interactions between the bacterial host and its plasmid(s) exist, no systematic study to date has compared the stability of the same plasmid among different hosts. the goal of this study was to exami ...200717259616
monitoring bacterial communities in raw milk and cheese by culture-dependent and -independent 16s rrna gene-based analyses.the diversity and dynamics of bacterial populations in saint-nectaire, a raw-milk, semihard cheese, were investigated using a dual culture-dependent and direct molecular approach combining single-strand conformation polymorphism (sscp) fingerprinting and sequencing of 16s rrna genes. the dominant clones, among 125 16s rrna genes isolated from milk, belonged to members of the firmicutes (58% of the total clones) affiliated mainly with the orders clostridiales and the lactobacillales, followed by ...200717259356
infectious complications of percutaneous central venous catheterization in pediatric patients: a spanish multicenter study.analysis of infectious complications and risk factors in percutaneous central venous catheters.200717235512
immunostimulatory properties of the emerging pathogen stenotrophomonas maltophilia.stenotrophomonas maltophilia is a multiple-antibiotic-resistant opportunistic pathogen that is being isolated with increasing frequency from patients with health-care-associated infections and especially from patients with cystic fibrosis (cf). while clinicians feel compelled to treat infections involving this organism, its potential for virulence is not well established. we evaluated the immunostimulatory properties and overall virulence of clinical isolates of s. maltophilia using the well-cha ...200717220304
skin manifestation of stenotrophomonas maltophilia infection--a case report and review article.stenotrophomonas maltophilia is an aerobic gram-negative bacillus that is a frequent coloniser of fluids used in the hospital setting. it causes infection in immunosuppressed hosts, especially those who are neutropaenic, on chemotherapy and broad spectrum antibiotics. skin and soft tissue manifestations of stenotrophomonas maltophilia infection are becoming an increasingly recognised entity; the clinical spectrum ranges from mucocutaneous, skin to soft tissue infections.200617219003
enhanced hydroxylation of imidacloprid by stenotrophomonas maltophilia upon addition of sucrose.sucrose's ability to promote the hydroxylation of imidacloprid (imi) by bacterium stenotrophomonas maltophilia strain cgmcc 1.1788 was examined. both growing culture and resting cells could transform imi into 5-hydroxy imi. adding 2% sucrose to the growing culture transformation broth and 5% sucrose to the resting cell transformation broth resulted in biotransformation yields, respectively, 2.5 and 9 times greater than without sucrose. in the growing culture transformation, sucrose increased bio ...200717216456
microbial communities in the larval midgut of laboratory and field populations of cotton bollworm (helicoverpa armigera).we compared the bacterial communities in the larval midgut of field and laboratory populations of a polyphagous pest, the cotton bollworm (helicoverpa armigera), using denaturing gradient gel electrophoresis (dgge) of amplified 16s rdna sequences and 16s library sequence analysis. dgge profiles and 16s rdna library sequence analysis indicated similar patterns of midgut microbial community structure and diversity: specific bacterial types existed in both populations, and a more diverse microbial ...200617215900
in vitro activity of tigecycline against clinical isolates of acinetobacter baumannii and stenotrophomonas maltophilia. 200717213264
[short communication: prevalence of extended spectrum beta-lactamases in gram negative rods: data of 2001-2004 period].this study was undertaken to determine the prevalence of extended spectrum beta-lactamase (esbl) production by gram negative rods (gnrs) isolated from various clinical specimens (3.240 urine, 336 pus, 277 feces, 83 blood, 38 sputum) sent to our hospital laboratory, between 2001-2004. of isolated bacteria 71.4% were identified as escherichia coli, 11.1% were klebsiella spp, 4.2% were salmonella spp, 3.7% were pseudomonas spp, 3.5% were proteus spp, 2.9% were shigella spp, 2.3% were enterobacter s ...200617205693
stenotrophomonas maltophilia pneumonia in cancer patients without traditional risk factors for infection, 1997-2004.in order to elucidate the spectrum of stenotrophomonas maltophilia pneumonia in cancer patients without traditional risk factors, 44 cancer patients (cases) with s. maltophilia pneumonia in whom s. maltophilia pneumonia risk factors were not present were compared with two s. maltophilia pneumonia risk groups (controls) including 43 neutropenic non-intensive care unit (icu) and 21 non-neutropenic icu patients. the case and control patients had similar demographic and underlying clinical character ...200717200840
the molecular epidemiology of stenotrophomonas maltophilia bacteraemia in a tertiary referral hospital in the united arab emirates 2000-2004.stenotrophomonas maltophilia is recognised as an important cause of nosocomial infection, especially in immunocompromised patients, resulting in significant morbidity and mortality. the treatment of s. maltophilia infection presents a therapeutic challenge. the precise modes of transmission of s. maltophilia in the hospital environment are not known and such knowledge is essential to target interventions to prevent spread. there are few published data on the patterns of nosocomial infection in t ...200617194302
volatiles of bacterial antagonists inhibit mycelial growth of the plant pathogen rhizoctonia solani.bacterial antagonists are bacteria that negatively affect the growth of other organisms. many antagonists inhibit the growth of fungi by various mechanisms, e.g., secretion of lytic enzymes, siderophores and antibiotics. such inhibition of fungal growth may indirectly support plant growth. here, we demonstrate that small organic volatile compounds (vocs) emitted from bacterial antagonists negatively influence the mycelial growth of the soil-borne phytopathogenic fungus rhizoctonia solani kühn. s ...200717180381
influence of harvest bacterial contamination on autologous peripheral blood progenitor cells post-transplant.microbiological contamination of manipulated blood products, including hematopoietic progenitors obtained from peripheral blood, is an infrequent but persistent problem in transplant units. the relevance of such contamination in causing patient infection has been reported as insignificant, but the effect on the post-transplant course has not been well documented. we studied the incidence of bacterial contamination in autologous peripheral blood progenitor cell transplants in two of the bench pro ...200717173054
camphor-related self-inflicted keratoconjunctivitis complicating delusions of parasitosis.to report a case of camphor-related self-inflicted keratoconjunctivitis secondary to delusions of parasitosis.200617172913
infectious keratitis caused by stenotrophomonas maltophilia and yeast simultaneously.to report a case of coinfection of the human cornea by stenotrophomonas maltophilia and yeast.200617172906
serological cross-reactivity of environmental isolates of enterobacter, escherichia, stenotrophomonas, and aerococcus with shigella spp.-specific antisera.using protocols designed for the isolation of shigella from environmental freshwater samples from different regions of bangladesh, 11 bacterial strains giving rise to shigella-like colonies on selective agar plates and showing serological cross-reaction with shigella-specific antisera were isolated. phylogenetic analyses revealed that three of the isolates were most closely related to escherichia coli, four to enterobacter sp., two to stenotrophomonas, and two isolates belonged to the gram-posit ...200717171463
capillary isoelectric focusing and fluorometric detection of proteins and microorganisms dynamically modified by poly(ethylene glycol) pyrenebutanoate.the nonionogenic pyrene-based tenside, poly(ethylene glycol) pyrenebutanoate, was prepared and applied in capillary isoelectric focusing with fluorometric detection. this dye was used here as a buffer additive in capillary isoelectric focusing for a dynamic modification of the sample of proteins and microorganisms. the values of the isoelectric points of the labeled bioanalytes were calculated with use of the fluorescent pi markers and were found comparable with pi of the native compounds. the m ...200617165837
meningitis caused by stenotrophomonas maltophilia: case report and review of the literature. 200217159399
bacteria associated with orchid roots and microbial production of auxin.associative bacteria of terrestrial (paphiopedilum appletonianum) and epiphytic (pholidota articulata) tropical orchids were investigated. microbial community of epiphytic plant differed from that of the terrestrial one. streptomyces, bacillus, pseudomonas, burkholderia, erwinia and nocardia strains populated paphiopedilum roots, whereas pseudomonas, flavobacterium, stenotrophomonas, pantoea, chryseobacterium, bacillus, agrobacterium, erwinia, burkholderia and paracoccus strains colonized pholid ...200717140781
multidrug-resistant organisms in cystic fibrosis: management and infection-control issues.chronic infection and inflammation are the hallmarks of cystic fibrosis lung disease. as cystic fibrosis patients are living longer owing to more intense treatment, multidrug-resistant organisms are being isolated increasingly from patients' respiratory tracts. while the adverse effects of pseudomonas aeruginosa and burkholderia cepacia complex are well described, less is known about the clinical significance of other emerging multidrug-resistant organisms, such as methicillin-resistant staphylo ...200617140357
characterization of small-colony-variant stenotrophomonas maltophilia isolated from the sputum specimens of five patients with cystic fibrosis.cystic fibrosis (cf) patients are predisposed to chronic respiratory infection by nonfermentative gram-negative bacilli, including stenotrophomonas maltophilia. s. maltophilia is highly resistant to most antibiotics, with the exception of sulfamethoxazole-trimethoprim (sxt). sxt-resistant s. maltophilia has been reported, but the mechanism of resistance is not well defined. repeated findings of suspected small-colony-variant (scv) s. maltophilia isolates from the sputa of five cf patients were c ...200717135443
first reported infections caused by three newly described genera in the family xanthomonadaceae.members of the family of xanthomonadaceae are typically characterized as environmental organisms. with the exception of stenotrophomonas maltophilia, these organisms are infrequently implicated as human pathogens. we describe three cases of central venous catheter-associated bloodstream infections caused by dokdonella koreensis, aquimonas voraii, and a luteibacter sp., all newly named genera within the family xanthomonadaceae. the three patients all had histories of underlying hematological diso ...200717122001
cloning of mpd gene from a chlorpyrifos-degrading bacterium and use of this strain in bioremediation of contaminated soil.an effective chlorpyrifos-degrading bacterium (named strain yc-1) was isolated from the sludge of the wastewater treating system of an organophosphorus pesticides manufacturer. based on the results of phenotypic features, phylogenetic similarity of 16s rrna gene sequences and biolog test, strain yc-1 was identified as the genus stenotrophomonas. the isolate utilized chlorpyrifos as the sole source of carbon and phosphorus for its growth and hydrolyzed chlorpyrifos to 3,5,6-trichloro-2-pyridinol. ...200617107423
bacterial diversity in aortic aneurysms determined by 16s ribosomal rna gene analysis.aortic aneurysms are common vascular conditions that cause considerable morbidity and mortality. understanding of the mechanisms involved in the pathogenesis of the condition remains limited. recently, infection has been suggested as possible contributor in the development of the disease. the aim of the present study was to examine aortic aneurysms for the presence of bacterial dna using polymerase chain reaction (pcr) targeting the 16s ribosomal rna (rrna) gene, followed by cloning and sequenci ...200617098542
aph(3')-iic, an aminoglycoside resistance determinant from stenotrophomonas maltophilia.we report the characterization of an intrinsic, chromosomally carried aph(3')-iic gene from stenotrophomonas maltophilia clinical isolate k279a, encoding an aminoglycoside phosphotransferase enzyme that significantly increases mics of kanamycin, neomycin, butirosin, and paromomycin when expressed in escherichia coli. disruption of aph(3')-iic in k279a results in decreased mics of these drugs.200717088477
the immunomodulatory effect of antibiotics on the secretion of tumour necrosis factor alpha by peripheral blood mononuclear cells in response to stenotrophomonas maltophilia stimulation.some antibiotics have been shown to modify the host immune response. infection with stenotrophomonas maltophilia, is often difficult to treat due to multiresistance to antibiotics. the authors examined the effect of four commonly used antimicrobial agents (ciprofloxacin, ceftazidime, cotrimoxazole and piperacillin-tazobactam) on tumour necrosis factor alpha (tnf alpha) production by human peripheral blood mononuclear cells (pbmc) stimulated with heat-killed s maltophilia. cotrimoxazole was the o ...200617087095
preparation of 3-ketovalidoxylamine a c-n lyase substrate: n-p-nitrophenyl-3-ketovalidamine by stenotrophomonas maltrophilia cctcc m 204024.3-ketovalidoxylamine a c-n lyase is one of three key enzymes in the production of valienamine, which is a potent glucosidase inhibitor from validamycin a. n-p-nitrophenyl-3-ketovalidamine, used as the substrate of 3-ketovalidoxylamine a c-n lyase, was prepared from n-p-nitrophenylvalidamine with free cells of stenotrophomonas maltrophilia cctcc m 204024. the yield and selectivity of n-p-nitrophenyl-3-ketovalidamine from cells were improved by treatment with 10 mm ethylenediaminetetraacetic acid. ...200717058074
Displaying items 601 - 700 of 1621