Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| genomic motifs as a novel indicator of the relationship between strains isolated from the epidemic of porcine epidemic diarrhea in 2013-2014. | porcine epidemic diarrhea virus (pedv) is a positive-sense rna virus that causes infectious gastroenteritis in pigs. following a ped outbreak that occurred in china in 2010, the disease was identified for the first time in the united states in april 2013, and was reported in many other countries worldwide from 2013 to 2014. as a novel approach to elucidate the epidemiological relationship between pedv strains, we explored their genome sequences to identify the motifs that were shared within rela ... | 2016 | 26808527 |
| molecular characterization of the spike and orf3 genes of porcine epidemic diarrhea virus in the philippines. | porcine epidemic diarrhea virus (pedv) is a highly contagious enteric pathogen of swine. in the present study, we analyzed the spike genes and orf3 genes of seven pedv strains detected in philippine pigs in june 2014. there are four major epitope regions in the spike glycoprotein: a co-26k equivalent (coe) domain, ss2 and ss6 epitopes, and an epitope region recognized by the 2c10 monoclonal antibody. analysis of philippine strains revealed amino acid substitutions in the ss6 epitope region (lqdg ... | 2016 | 26801789 |
| suppression of type i interferon production by porcine epidemic diarrhea virus and degradation of creb-binding protein by nsp1. | type i interferons (ifn-α/β) are the major components of the innate immune response of hosts, and in turn many viruses have evolved to modulate the host response during infection. we found that the ifn-β production was significantly suppressed during pedv infection in cells. to identify viral ifn antagonists and to study their suppressive function, viral coding sequences for the entire structural and nonstructural proteins were cloned and expressed. of 16 pedv nonstructural proteins (nsps), nsp1 ... | 2016 | 26773386 |
| development of a neutralizing monoclonal antibody against porcine epidemic diarrhea virus s1 protein. | the truncated spike protein (s1, 390-789aa) of porcine epidemic diarrhea virus was expressed as a recombinant protein in baculorvirus expression system. female balb/c mice were immunized with the purified recombinant s1 (rs1) protein and two monoclonal antibodies, designated 8a3a10 and 12c4g12, were generated by hybridoma technique. the two monoclonal antibodies specifically reacted with pedv rs1 protein, as proven by western blot and indirect immunofluorescence assay. 8a3a10 monoclonal antibody ... | 2016 | 26771404 |
| impact of mated female nonproductive days in breeding herd after porcine epidemic diarrhea virus outbreak. | porcine epidemic diarrhea virus (pedv) is an important pathogen that has a significant economic impact on the swine industry by imposing a high rate of mortality in suckling piglets. however, limited information on the productivity values of gilts and sows infected with pedv is available. here, we evaluate the productivity index in gilts and sows during the 1-year period before (19 january 2013 to 18 january 2014) and after (19 january 2014 to 18 january 2015) a pedv outbreak from a 2000-sow bre ... | 2016 | 26771383 |
| case-control study of pathogens involved in piglet diarrhea. | diarrhea in piglets directly affects commercial swine production. the disease results from the interaction of pathogens with the host immune system and is also affected by management procedures. several pathogenic agents such as campylobacter spp., clostridium perfringens, escherichia coli, salmonella spp., group a rotavirus (rv-a), coronaviruses (transmissible gastroenteritis virus; porcine epidemic diarrhea virus), as well as nematode and protozoan parasites, can be associated with disease cas ... | 2016 | 26754836 |
| production impact & time to stability in sow herds infected with porcine epidemic diarrhea virus (pedv). | pedv was first detected in united states in may, 2013. the virus spread through the swine industry and was reported in 30 us states by june, 2014 (morrison and goede, 2014). there are limited data describing the impact on production in sow farms. veterinarians attempt to control the virus in sow herds with a program that stimulates herd immunity. there are no data on how long it takes with this control program to achieve a stable state of consistently produce weaned pigs that are not infected wi ... | 2016 | 26748596 |
| genome sequencing and analysis of a novel recombinant porcine epidemic diarrhea virus strain from henan, china. | porcine epidemic diarrhea virus (pedv) has caused devastating impact on pig-rearing industry in china and current vaccine is not effective against the circulating pedv variants. in the present study, the full-length genome sequence from a pedv isolate (ch/hnqx-3/14) was determined. the complete genome sequence analysis showed that the ch/hnqx-3/14 possessed unique deletion regions in the s and orf3 genes. it was identified as a recombinant strain using phylogenetic analysis and recombination det ... | 2016 | 26743534 |
| outbreak investigation of porcine epidemic diarrhea in swine in ontario. | porcine epidemic diarrhea virus was first diagnosed in ontario in january of 2014. an outbreak investigation was conducted and it was hypothesized that feed containing spray-dried porcine plasma contaminated with the virus was a risk factor in the introduction and spread of the disease in ontario. | 2016 | 26740705 |
| molecular detection of porcine astrovirus in sichuan province, china. | porcine astrovirus (poastv) is widely distributed worldwide, and is highly prevalent among piglets with or without diarrhea, existing as at least five distinct lineages (poastv1-poastv5) within the genus mamastrovirus. however, our knowledge of the diversity and epidemiology of poastv in china is limited. | 2016 | 26739067 |
| characterization of a pathogenic full-length cdna clone and transmission model for porcine epidemic diarrhea virus strain pc22a. | porcine epidemic diarrhea virus (pedv) is a highly pathogenic alphacoronavirus. in the united states, highly virulent pedv strains cause between 80 and 100% mortality in suckling piglets and are rapidly transmitted between animals and farms. to study the genetic factors that regulate pathogenesis and transmission, we developed a molecular clone of pedv strain pc22a. the infectious-clone-derived pedv (icpedv) replicated as efficiently as the parental virus in cell culture and in pigs, resulting i ... | 2016 | 26733065 |
| first detection, clinical presentation and phylogenetic characterization of porcine epidemic diarrhea virus in austria. | porcine epidemic diarrhea (ped) is a syndrome that is characterized by rapidly spreading watery diarrhea affecting pigs of all ages, but with major effects on suckling piglets. the disease, as well as the causative alphacoronavirus, the porcine epidemic diarrhea virus (pedv), was first described in europe in the 1970s and since then has spread over many asian and american countries, where it recently led to devastating effects on swine health and pork industry. while the disease was seldom repor ... | 2015 | 26714453 |
| porcine epidemic diarrhea virus introduction into the united states: root cause investigation. | porcine epidemic diarrhea (ped) was identified in the united states in the spring of 2013, and professionals from many parts of the u.s. swine industry responded rapidly to understand and control the newly emerging disease. in less than two months, the disease had spread to more than 200 herds in thirteen states. experts from the us department of agriculture (usda) engaged in laboratory diagnostics, analytic support, epidemiology expertise, and data management to facilitate the effort. by 2014, ... | 2016 | 26711302 |
| a porcine epidemic diarrhea virus outbreak in one geographic region of the united states: descriptive epidemiology and investigation of the possibility of airborne virus spread. | this study describes a spring 2013 outbreak of porcine epidemic diarrhea virus (pedv), using data from 222 swine sites in 14 counties area in 4 contiguous states in the united states. during the outbreak, the premises-level incidence of pedv was 40.5 percent (90/222 sites). one of the three companies from which data were collected had a lower incidence (19.5 percent) than the other two companies (41.1 and 47.2 percent). sow sites had the highest incidence of pedv during the outbreak (80.0 percen ... | 2015 | 26709512 |
| evaluation of two real-time polymerase chain reaction assays for porcine epidemic diarrhea virus (pedv) to assess pedv transmission in growing pigs. | in april 2013, a porcine epidemic diarrhea virus (pedv) epidemic began in the united states. as part of the response, real-time reverse transcription polymerase chain reaction (rt-pcr) assays to detect pedv were developed by several veterinary diagnostic laboratories. our study evaluated rt-pcr pedv assays that detect the n gene (gn) and s gene (gs) for their ability to detect pedv infection and the transmission potential of pigs experimentally exposed to pedv. detection limits and quantificatio ... | 2016 | 26699519 |
| antiviral indolosesquiterpenoid xiamycins c-e from a halophilic actinomycete. | new metabolites, xiamycins c-e (1-3), were isolated from a streptomyces. sp (#hk18) culture inhabiting the topsoil in a korean solar saltern. the planar structures of the xiamycins c-e were elucidated as carbazole-bearing indolosesquiterpenoids using a combined analysis of nmr, ms, uv, and ir spectroscopic data. the absolute configurations of these new compounds were determined by analyses of noesy and ecd data. when the xiamycins were tested for inhibitory activity on porcine epidemic diarrhea ... | 2016 | 26698879 |
| us-like isolates of porcine epidemic diarrhea virus from japanese outbreaks between 2013 and 2014. | since late 2013, outbreaks of porcine epidemic diarrhea virus (pedv) have reemerged in japan. in the present study, we observed a high detection rate of pedv, with 72.5 % (148/204) of diarrhea samples (suckling, weaned, and sows) and 88.5 % (77/87) of farms experiencing acute diarrhea found to be positive for pedv by reverse transcription pcr. sequencing and phylogenic analyses of the partial spike gene and orf3 of pedv demonstrated that all prevailing japanese pedv isolates belonged to novel ge ... | 2015 | 26693114 |
| coronaviruses: emerging and re-emerging pathogens in humans and animals. | the severe acute respiratory syndrome coronavirus (sars-cov) and recently emerged middle east respiratory syndrome coronavirus (mers-cov) epidemics have proven the ability of coronaviruses to cross species barrier and emerge rapidly in humans. other coronaviruses such as porcine epidemic diarrhea virus (pedv) are also known to cause major disease epidemics in animals with huge economic loss. this special issue in virology journal aims to highlight the advances and key discoveries in the animal o ... | 2015 | 26690088 |
| porcine epidemic diarrhea virus: an emerging and re-emerging epizootic swine virus. | the enteric disease of swine recognized in the early 1970s in europe was initially described as "epidemic viral diarrhea" and is now termed "porcine epidemic diarrhea (ped)". the coronavirus referred to as ped virus (pedv) was determined to be the etiologic agent of this disease in the late 1970s. since then the disease has been reported in europe and asia, but the most severe outbreaks have occurred predominantly in asian swine-producing countries. most recently, ped first emerged in early 2013 ... | 2015 | 26689811 |
| porcine epidemic diarrhea virus and discovery of a recombinant swine enteric coronavirus, italy. | porcine epidemic diarrhea virus (pedv) has been detected sporadically in italy since the 1990s. we report the phylogenetic relationship of swine enteric coronaviruses collected in italy during 2007-2014 and identify a drastic shift in pedv strain variability and a new swine enteric coronavirus generated by recombination of transmissible gastroenteritis virus and pedv. | 2016 | 26689738 |
| isolation and experimental inoculation of an s indel strain of porcine epidemic diarrhea virus in japan. | in 2013, porcine epidemic diarrhea (ped) was reported in japan for the first time in 7years and caused significant economic losses. in the present study, we isolated ped virus (pedv) circulating in japan using vero cell cultures and analyzed sequences of s1 genes of these pedv isolates. sequence analysis revealed that one of these strains contained distinct insertion and deletions in the s gene (i.e., s indel). furthermore, inoculation of pedv into 1-week-old pigs demonstrated that the s indel s ... | 2015 | 26679803 |
| complete genome sequence of the porcine epidemic diarrhea virus variant ch/hnyf/2014. | sow's milk is a potential route for the vertical transmission of porcine epidemic diarrhea virus (pedv) from sow to suckling piglet. we report here the complete genome sequence of pedv strain ch/hnyf/2014, which was isolated from milk samples : this information provides further understanding of the transmission mechanisms and genetic diversity of pedv. | 2015 | 26679593 |
| a recombinant nucleocapsid protein-based indirect enzyme-linked immunosorbent assay to detect antibodies against porcine deltacoronavirus. | recently, porcine deltacoronavirus (pdcov) has been proven to be associated with enteric disease in piglets. diagnostic tools for serological surveys of pdcov remain in the developmental stage when compared with those for other porcine coronaviruses. in our study, an indirect enzyme-linked immunosorbent assay (elisa) (rpdcov-n-elisa) was developed to detect antibodies against pdcov using a histidine-tagged recombinant nucleocapsid (n) protein as an antigen. the rpdcov-n-elisa did not cross-react ... | 2016 | 26668175 |
| evaluation of humoral immune status in porcine epidemic diarrhea virus (pedv) infected sows under field conditions. | porcine epidemic diarrhea virus (pedv) is an economically devastating enteric disease in the swine industry. the virus infects pigs of all ages, but it cause severe clinical disease in neonatal suckling pigs with up to 100% mortality. currently, available vaccines are not completely effective and feedback methods utilizing pedv infected material has variable success in preventing reinfection. comprehensive information on the levels and duration of effector/memory iga and igg antibody secreting b ... | 2015 | 26667229 |
| porcine epidemic diarrhea virus 3c-like protease regulates its interferon antagonism by cleaving nemo. | porcine epidemic diarrhea virus (pedv) is an enteropathogenic coronavirus causing lethal watery diarrhea in piglets. since 2010, a pedv variant has spread rapidly in china, and it emerged in the united states in 2013, posing significant economic and public health concerns. the ability to circumvent the interferon (ifn) antiviral response, as suggested for pedv, promotes viral survival and regulates pathogenesis of pedv infections, but the underlying mechanisms remain obscure. here, we show that ... | 2015 | 26656704 |
| elevated plasma-soluble cd16 levels in porcine reproductive and respiratory syndrome virus-infected pigs: correlation with adam17-mediated shedding. | soluble cd16 (scd16) is closely correlated with chronic diseases in humans. here, plasma scd16 levels in pigs were increased by infection with porcine reproductive and respiratory syndrome virus (prrsv) but not with porcine epidemic diarrhea virus, porcine circovirus type 2 and pseudorabies virus. of interest, prrsv attached to blood neutrophils and reduced surface cd16 expression on neutrophils. in vitro data confirmed that prrsv caused cd16 shedding in neutrophils. further analyses revealed th ... | 2016 | 26653711 |
| assessment of the economic impacts of porcine epidemic diarrhea virus in the united states. | porcine epidemic diarrhea virus (pedv), which first emerged in the united states in 2013, spread throughout the u.s. hog population. limited preemptive knowledge impeded the understanding of pedv introduction, spread, and prospective economic impacts in the united states. to assess these impacts, this article reviews the timeline of pedv in the united states and the corresponding impacts. pedv is a supply-impacting disease and is not demand inhibiting, as pork demand remained strong since pedv f ... | 2015 | 26641031 |
| immunogenicity and antigenic relationships among spike proteins of porcine epidemic diarrhea virus subtypes g1 and g2. | porcine epidemic diarrhea virus (pedv) is a coronavirus that infects cells lining the small intestine of swine, resulting in vomiting, diarrhea, and dehydration. the amino acid sequence of the spike (s) protein, which is the principal target recognized by host immune cells, has multiple mutations that distinguish the two pedv genotypes, g1 and g2. to determine whether these mutations lead to changes in antigenicity, as suggested by the failure of pedv vaccines in china, we first optimized the co ... | 2016 | 26611909 |
| genomic and evolutionary inferences between american and global strains of porcine epidemic diarrhea virus. | porcine epidemic diarrhea virus (pedv) has caused severe economic losses both recently in the united states (us) and historically throughout europe and asia. traditionally, analysis of the spike gene has been used to determine phylogenetic relationships between pedv strains. we determined the complete genomes of 93 pedv field samples from us swine and analyzed the data in conjunction with complete genome sequences available from genbank (n=126) to determine the most variable genomic areas. our r ... | 2016 | 26611651 |
| retrospective testing and case series study of porcine delta coronavirus in u.s. swine herds. | porcine deltacoronavirus (pdcov) was first reported in the united states (us) in february 2014. this was the second novel swine enteric coronavirus detected in the us since may 2013. in this study, we conducted retrospective testing of samples submitted to three veterinary diagnostic laboratories where qualifying biological samples were derived from previously submitted diagnostic case submissions from us commercial swine farms with a clinical history of enteric disease or from cases that had be ... | 2016 | 26603048 |
| sensitivity of porcine epidemic diarrhea virus (pedv) to ph and heat treatment in the presence or absence of porcine plasma. | emergence of porcine epidemic diarrhea virus (pedv) resulted in massive neonatal mortality in the north-american and asian pork industry. measures to prevent its geographical spread are of utmost importance to safeguard susceptible porcine populations. the major infection route is direct or indirect faecal-oral contact. adequate biosafety measures should be in place at all levels of the swine production chain, including feed and feed ingredients. present study aimed to investigate the sensitivit ... | 2015 | 26602207 |
| experimental infection of a us spike-insertion deletion porcine epidemic diarrhea virus in conventional nursing piglets and cross-protection to the original us pedv infection. | although the original us porcine epidemic diarrhea virus (pedv) was confirmed as highly virulent by multiple studies, the virulence of spike-insertion deletion (s-indel) pedv strains is undefined. in this study, 3-4 day-old conventional suckling piglets were inoculated with s-indel pedv iowa106 (4 pig litters) to study its virulence. two litters of age-matched piglets were inoculated with either the original us pedv pc21a or mock as positive and negative controls, respectively. subsequently, all ... | 2015 | 26589292 |
| detection of immunoglobulin (ig) a antibodies against porcine epidemic diarrhea virus (pedv) in fecal and serum samples. | many assays for detection of antibodies against porcine epidemic diarrhea virus (pedv) are based on detection of neutralizing antibodies or immunoglobulin (ig) g in serum samples. however, due to the particular features of the mucosal immune system, presence of serum antibodies against enteric pathogens, such as pedv, not always correlates with protection. in contrast, anti-pedv iga antibodies correlate with protection against subsequent challenges. an indirect pedv iga elisa was previously deve ... | 2015 | 26587386 |
| spatial and temporal epidemiology of porcine epidemic diarrhea (ped) in the midwest and southeast regions of the united states. | emergence of porcine epidemic diarrhea virus (pedv) in the us in 2013 caused a major impact in the swine industry due to its high mortality and rapid spread through the country. even though the role of potential sources of infection in the epidemiology of the disease at the farm level (feed, fomites) has been extensively investigated, there is a lack of knowledge about the dynamics of disease spread at the regional level. here, we investigated the dissemination of pedv infection in two areas loc ... | 2016 | 26586344 |
| isolation of porcine epidemic diarrhea virus during outbreaks in south korea, 2013-2014. | 2015 | 26584230 | |
| porcine epidemic diarrhea virus among farmed pigs, ukraine. | an outbreak of porcine epidemic diarrhea occurred in the summer of 2014 in ukraine, severely affecting piglets <10 days of age; the mortality rate approached 100%. full genome sequencing showed the virus to be closely related to strains reported from north america, showing a sequence identity of up to 99.8%. | 2015 | 26584081 |
| epidemiology and vaccine of porcine epidemic diarrhea virus in china: a mini-review. | porcine epidemic diarrhea (ped) is an intestinal infectious disease caused by porcine epidemic diarrhea virus (pedv); manifestations of the disease are diarrhea, vomiting and dehydration. starting from the end of 2010, a ped outbreak occurred in several pig-producing provinces in southern china. subsequently, the disease spread throughout the country and caused enormous economic losses to the pork industry. accumulating studies demonstrated that new pedv variants that appeared in china were resp ... | 2016 | 26537549 |
| sequence and phylogenetic analyses of the m and n genes of porcine epidemic diarrhea virus (pedv) strains in anhui province, china. | to assess the homology and phylogenetic relationship between porcine epidemic diarrhea virus (pedv) anhui strains and other pedv strains, molecular homology and phylogenetic analyses of anhui pedv field strains were compared with those of reference strains. the results revealed that the m and n genes of pedv were 681 and 1326 bp long, respectively. the nucleotide sequences of the n genes of anhui pedv strains were 95.9-99.9% homologous with each other, and the deduced amino acid sequences were 9 ... | 2015 | 26535655 |
| porcine epidemic diarrhea virus orf3 gene prolongs s-phase, facilitates formation of vesicles and promotes the proliferation of attenuated pedv. | porcine epidemic diarrhea virus (pedv) is a porcine enteropathogenic coronavirus that has received increasing attention since the emergence of a pedv variant worldwide. previous studies have shown that pedv orf3 encodes an ion channel protein. however, its influence on cell cycle and subcellular structure still require more research. in this study, we developed a vero cell line that stably expresses pedv orf3 gene. subcellular localization and influences of pedv orf3 on host cells were investiga ... | 2015 | 26531166 |
| comparative genomic analysis of classical and variant virulent parental/attenuated strains of porcine epidemic diarrhea virus. | since 2010, the variant porcine epidemic diarrhea virus (pedv) has been the etiological agent responsible for the outbreak of porcine epidemic diarrhea (ped) worldwide. in this study, a variant pedv strain yn1 was isolated, serially propagated on the vero cells and was characterized for 200 passages. to better elucidate the molecular basis of vero cell adaptation of variant pedv strains, we sequenced, compared, and analyzed the full-genome sequences of parental yn1 and passages 15, 30, 60, 90, 1 ... | 2015 | 26512689 |
| prokaryotic expression of truncated s1 protein of porcine epidemic diarrhea virus and production of monoclonal antibodies to recombinant protein. | monoclonal antibodies are known to have several applications in clinical diagnosis and therapy. in the present study, the truncated s1 gene, encoding the exterior of the viral spike protein of porcine epidemic diarrhea virus (pedv), was subcloned into prokaryotic expression vector pet32a (+) and expressed as a recombinant protein in escherichia coli bl21(de3). female balb/c mice were immunized with the purified recombinant truncated s1 protein, and three monoclonal antibodies (mab designated as ... | 2015 | 26492620 |
| comparison between the 2013-2014 and 2009-2012 annual porcine reproductive and respiratory syndrome virus epidemics in a cohort of sow herds in the united states. | the purpose of this study was to describe the 2013/2014 porcine reproductive and respiratory syndrome virus (prrsv) epidemic in the united states and compare it with the previous 4 y of data from 2009 to 2012. a total of 371 herds participated in the study, representing nearly 1.2 million sows in 15 states. there were significantly fewer prrsv cases during this study period and the onset of the annual epidemic was delayed approximately 3 wk. cluster analysis revealed a pattern similar to previou ... | 2015 | 26483586 |
| molecular characterization of pig epidemic diarrhoea viruses isolated in japan from 2013 to 2014. | since october 2013, approximately 1000 outbreaks of porcine epidemic diarrhoea (ped) have occurred, spanning almost all prefectures of japan, after a period of seven years without a reported case. in order to consider occurrence factor of ped outbreaks, we determined the whole-genome sequences of 38 ped virus (pedv) strains from diarrheal samples collected at swine farms in 18 prefectures between 2013 and 2014 using next-generation sequencing technology. using these data, we investigated genetic ... | 2015 | 26477934 |
| medicinal herb extracts ameliorate impaired growth performance and intestinal lesion of newborn piglets challenged with the virulent porcine epidemic diarrhea virus. | the objective of this study was to evaluate effects of a combined use of extracts of medicinal herbs taraxaumi mongolicum, viola yedoensis makino, rhizoma coptidis, and radix isatidis (myci) on porcine epidemic diarrhea (ped). twenty-two 3-day-old piglets received an oral challenge with 3 × 10(3.5) tcid50 of the virulent ped virus (pedv) in pbs or pbs only and daily oral administration of 60 mg of the myci mixture suspended in milk replacer or the vehicle for 7 days in a 2 × 2 factorial arrangem ... | 2015 | 26451253 |
| effect of porcine epidemic diarrhea virus infectious doses on infection outcomes in naïve conventional neonatal and weaned pigs. | porcine epidemic diarrhea virus (pedv) was identified in the united states (u.s.) swine population for the first time in april 2013 and rapidly spread nationwide. however, no information has been published regarding the minimum infectious dose (mid) of pedv in different pig models. the main objective of this study was to determine the oral minimum infectious dose of pedv in naïve conventional neonatal piglets and weaned pigs. a u.s. virulent pedv prototype isolate (usa/in19338/2013) with known i ... | 2015 | 26441071 |
| porcine epidemic diarrhea virus m protein blocks cell cycle progression at s-phase and its subcellular localization in the porcine intestinal epithelial cells. | porcine epidemic diarrhea (ped) caused by virulent strains of porcine epidemic diarrhea virus (pedv) is a highly contagious enteric disease of swine characterized by severe enteritis, vomiting, and watery diarrhea. this study investigates the subcellular localization and function of pedv m protein through examination of its effects on cell growth, cell cycle progression, and interleukin 8 (il-8) expression. our results revealed that the pedv m protein is localized throughout the cytoplasm. the m ... | 2015 | 26435150 |
| age-dependent variation in innate immune responses to porcine epidemic diarrhea virus infection in suckling versus weaned pigs. | porcine epidemic diarrhea (ped) is an enteric coronaviral infection that causes severe morbidity and mortality in suckling pigs, but less severe disease in older pigs. consequently, it causes significant economic losses to the pork industry. there are limited studies on the innate immune responses to ped virus (pedv) in pigs. the aims of our study were to investigate differences in innate immune responses to pedv infection in suckling and weaned pigs and to examine if disease severity coincides ... | 2015 | 26433606 |
| determination of the infectious titer and virulence of an original us porcine epidemic diarrhea virus pc22a strain. | the infectious dose of a virus pool of original us pedv strain pc22a was determined in 4-day-old, cesarean-derived, colostrum-deprived (cdcd) piglets. the median pig diarrhea dose (pdd50) of the virus pool was determined as 7.35 log10 pdd50/ml, similar to the cell culture infectious titer, 7.75 log10 plaque-forming units (pfu)/ml. 100 pdd50 caused watery diarrhea in all conventional suckling piglets (n = 12) derived from a pedv-naive sow, whereas 1000 and 10 000 pdd50 did not cause diarrhea in p ... | 2015 | 26408019 |
| evolutionary characterization of the emerging porcine epidemic diarrhea virus worldwide and 2014 epidemic in taiwan. | since 2010, a new variant of pedv belonging to genogroup 2 has been transmitting in china and further spreading to the unites states and other asian countries including taiwan. in order to characterize in detail the temporal and geographic relationships among pedv strains, the present study systematically evaluated the evolutionary patterns and phylogenetic resolution in each gene of the whole pedv genome in order to determine which regions provided the maximal interpretative power. the result w ... | 2015 | 26375730 |
| antiviral activity of graphene oxide: how sharp edged structure and charge matter. | graphene oxide and its derivatives have been widely explored for their antimicrobial properties due to their high surface-to-volume ratios and unique chemical and physical properties. however, little information is available on their effects on viruses. in this study, we report the broad-spectrum antiviral activity of go against pseudorabies virus (prv, a dna virus) and porcine epidemic diarrhea virus (pedv, an rna virus). our results showed that go significantly suppressed the infection of prv ... | 2015 | 26370151 |
| jubanines f-j, cyclopeptide alkaloids from the roots of ziziphus jujuba. | five ib-type cyclopeptide alkaloids, jubanines f-j (1-5), and three known compounds, nummularine b (6), daechuine-s3 (7), and mucronine k (8) were isolated from the roots of ziziphus jujuba. their structures were fully characterized by spectroscopic analyses in combination with chemical derivatization. compounds 1-3, and 6 were evaluated for their antiviral activity against the porcine epidemic diarrhea virus (pedv). compounds 2, 3, and 6 showed potent inhibitory effects on pedv replication. | 2015 | 26361730 |
| itraq-based comparative proteomic analysis of vero cells infected with virulent and cv777 vaccine strain-like strains of porcine epidemic diarrhea virus. | the re-emerging porcine epidemic diarrhea virus (pedv) variant related diarrhea has been documented in china since late 2010 and now with global distribution. currently, a virulent pedv ch/ynkm-8/2013 and a cv777 vaccine strain-like ah-m have been successfully isolated from the clinical samples. to dissect out the underlying pathogenic mechanism of virulent pedv and clarify the differences between virulent and cv777 vaccine strain-like pedv infections, we performed an itraq-based comparative qua ... | 2016 | 26361011 |
| outbreak of porcine epidemic diarrhea virus in portugal, 2015. | an outbreak of porcine epidemic diarrhea virus (pedv) in the south of portugal in january 2015 and the spread of pedv northwards in the territory are described. comparative analysis of the amplified sequences showed a very high (99.0%) identity with the pedv variant most recently reported in the united states and also show complete (100%) identity to the strains recently reported in germany, supporting the hypothesis that a unique strain is currently circulating in europe. the origin of this ped ... | 2015 | 26344708 |
| a novel diagnostic approach to detecting porcine epidemic diarrhea virus: the lateral immunochromatography assay. | porcine epidemic diarrhea virus (pedv) causes acute diarrhea and dehydration in sucking piglets and has a high mortality rate. an immunochromatography (ic) assay, known as a lateral flow test, is a simple device intended to detect the presence of target pathogens. here, we developed an ic assay that detected pedv antigens with 96.0% (218/227) sensitivity and 98.5% (262/266) specificity when compared with real-time reverse transcriptase (rt)-pcr using fam-labeled probes based on sequences from nu ... | 2015 | 26342906 |
| effective inhibition of porcine epidemic diarrhea virus by rna interference in vitro. | porcine epidemic diarrhea virus (pedv) is a member of the coronaviridae family, which can cause acute and highly contagious enteric disease of swine characterized by severe entero-pathogenic diarrhea in piglets. currently, the vaccines of pedv are only partially effective and there is no specific drug available for treatment of pedv infection. to exploit the possibility of using rna interference (rnai) as a strategy against pedv infection, five shrna-expressing plasmids targeting the n, m, and s ... | 2015 | 26329934 |
| porcine epidemic diarrhea virus infection induced the unbalance of gut microbiota in piglets. | porcine epidemic diarrhea (ped) is a devastating disease in livestock industry. most of the previous studies related to the ped were focused on the pathology and etiology of porcine epidemic diarrhea virus (pedv). a little was known regarding the status of gut microbiota after piglets infected by pedv. in this study, aided by metagenome sequencing technology, gut microbiota profiles in feces of viral diarrhea (vd) and viral control (vc) piglets were investigated. the results showed that the abun ... | 2015 | 26319658 |
| the dilemma of rare events: porcine epidemic diarrhea virus in north america. | porcine epidemic diarrhea virus (pedv) has been recognized as a swine pathogen for 40 years, but until 2013 had not been detected in the western hemisphere. from originally causing a relatively mild and sporadic disease, pedv has been more recently associated with severe outbreaks of diarrheal disease in asia, and subsequently north america. pedv shares some important characteristics with two major pandemic viruses (porcine reproductive and respiratory virus; porcine circovirus type 2) of pigs t ... | 2015 | 26318527 |
| chicken egg yolk antibodies (igy) as non-antibiotic production enhancers for use in swine production: a review. | in recent years, the use of in-feed antibiotics for growth and disease prevention in livestock production has been under severe scrutiny. the use and misuse of in-feed antibiotics has led to problems with drug residues in animal products and increased bacterial resistance. chicken egg yolk antibodies (igy) have attracted considerable attention as an alternative to antibiotics to maintain swine health and performance. oral administration of igy possesses many advantages over mammalian igg such as ... | 2015 | 26309735 |
| development of an indirect elisa based on a truncated s protein of the porcine epidemic diarrhea virus. | porcine epidemic diarrhea (ped) is a highly contagious, enteric disease of swine caused by the porcine epidemic diarrhea virus (pedv). to find a suitable elisa method to assess the infection of pedv and the effectiveness of vaccines, we developed and evaluated an indirect enzyme-linked immunosorbent assay (ielisa) based on a truncated recombinant spike (s) protein expressed in escherichia coli. the parameters of the ielisa were optimized, and the cutoff value determined as 0.259 by analyzing opt ... | 2015 | 26308702 |
| putative phage-display epitopes of the porcine epidemic diarrhea virus s1 protein and their anti-viral activity. | porcine epidemic diarrhea virus (pedv) is a pathogen of swine that causes severe diarrhea and dehydration resulting in substantial morbidity and mortality in newborn piglets. phage display is a technique with wide application, in particular, the identification of key antigen epitopes for the development of therapeutic and diagnostic reagents and vaccines. to identify antigen epitopes with specificity for pedv, a monoclonal antibody (mab-5e12) against the immunodominant region of the pedv spike p ... | 2015 | 26292945 |
| concentration, size distribution, and infectivity of airborne particles carrying swine viruses. | when pathogens become airborne, they travel associated with particles of different size and composition. particle size determines the distance across which pathogens can be transported, as well as the site of deposition and the survivability of the pathogen. despite the importance of this information, the size distribution of particles bearing viruses emitted by infectious animals remains unknown. in this study we characterized the concentration and size distribution of inhalable particles that ... | 2015 | 26287616 |
| porcine epidemic diarrhea virus inhibits dsrna-induced interferon-β production in porcine intestinal epithelial cells by blockade of the rig-i-mediated pathway. | the lack of optimal porcine cell lines has severely impeded the study and progress in elucidation of porcine epidemic diarrhea virus (pedv) pathogenesis. vero cell, an african green monkey kidney cell line, was often used to isolate and propagate pedv. nonetheless, the target cells of pedv in vivo are intestinal epithelial cells, during infection, intestinal epithelia would be damaged and resulted in digestive disorders. the immune functions of porcine epithelial cells and interactions with othe ... | 2015 | 26283628 |
| porcine epidemic diarrhea: a review of current epidemiology and available vaccines. | porcine epidemic diarrhea virus (pedv), an alphacoronavirus in the family coronaviridae, causes acute diarrhea, vomiting, dehydration, and high mortality rates in neonatal piglets. pedv can also cause diarrhea, agalactia, and abnormal reproductive cycles in pregnant sows. although pedv was first identified in europe, it has resulted in significant economic losses in many asian swine-raising countries, including korea, china, japan, vietnam, and the philippines. however, from april 2013 to the pr ... | 2015 | 26273575 |
| complete genome sequence of the porcine epidemic diarrhea virus variant tottori2/jpn/2014. | porcine epidemic diarrhea virus (pedv) is a cause of diarrhea outbreaks at swine farms, causing vomiting, severe diarrhea, and mortality in piglets. we sequenced and analyzed the complete genome of recently isolated strains. tottori2/jpn/2014, one of the sequenced pedv strains, had a unique large deletion in the s gene. | 2015 | 26272566 |
| development of an enzyme-linked immunosorbent assay for the monitoring and surveillance of antibodies to porcine epidemic diarrhea virus based on a recombinant membrane protein. | the recent dramatic increase in reported cases of porcine epidemic diarrhea (ped) in pig farms is a potential threat to the global swine industry. therefore, the accurate diagnosis, serological monitoring, and surveillance of specific antibodies in pigs resulting from porcine epidemic diarrhea virus (pedv) infection or vaccination would be essential in helping to control the spread of ped. we developed and validated an indirect enzyme-linked immunosorbent assay (elisa) based on the recombinant m ... | 2015 | 26253335 |
| newly emerged porcine deltacoronavirus associated with diarrhoea in swine in china: identification, prevalence and full-length genome sequence analysis. | to identify and characterize aetiologic agent(s) associated with an outbreak of a severe diarrhoea in piglets in jiangxi, china, in march 2015, a nested reverse transcription-polymerase chain reaction (rt-pcr) for the detection of porcine deltacoronavirus (pdcov) was developed. a survey based on the nested rt-pcr established indicated that the monoinfection of pdcov (33.71%) and coinfection of pdcov (19.66%) with porcine epidemic diarrhoea virus (pedv) were common in diarrhoeal pigs in jiangxi, ... | 2015 | 26250097 |
| development of an indirect elisa, blocking elisa, fluorescent microsphere immunoassay and fluorescent focus neutralization assay for serologic evaluation of exposure to north american strains of porcine epidemic diarrhea virus. | recent, severe outbreaks of porcine epidemic diarrhea virus (pedv) in asia and north america highlight the need for well-validated diagnostic tests for the identification of pedv infected animals and evaluation of their immune status to this virus. pedv was first detected in the u.s. in may 2013 and spread rapidly across the country. some serological assays for pedv have been previously described, but few were readily available in the u.s. several u.s. laboratories quickly developed indirect flu ... | 2015 | 26232106 |
| changes in the swine gut microbiota in response to porcine epidemic diarrhea infection. | the gastrointestinal tract of mammals is a complex ecosystem with distinct environments and comprises hundreds of different types of bacterial cells. the gut microbiota may play a critical role in the gut health of the host. we herein attempted to identify a microbiota shift that may be affected by porcine epidemic diarrhea (ped). we observed significant differences in microbiota between the control and ped virus (pedv)-infected groups at both the phylum and genus level. most commensal bacteria ... | 2015 | 26212519 |
| isolation and characterization of a korean porcine epidemic diarrhea virus strain knu-141112. | severe outbreaks of porcine epidemic diarrhea virus (pedv) have re-emerged in korea and rapidly swept across the country, causing tremendous economic losses to producers and customers. despite the availability of pedv vaccines in the domestic market, the disease continues to plague the korean pork industry, raising issues regarding their protective efficacy and new vaccine development. therefore, pedv isolation in cell culture is urgently needed to develop efficacious vaccines and diagnostic ass ... | 2015 | 26196056 |
| rapid quantitation of porcine epidemic diarrhea virus (pedv) by virus counter. | virus counter was applied for the quantitation of porcine epidemic diarrhea virus (pedv); the working range and reproducibility of the quantification were characterized, and the performance of virus counter was evaluated using the plaque assay as the gold standard. in this study, the single burst curves of pedv were first constructed with the virus counter or plaque assay respectively, and the results from virus counter and plaque assay showed similar high correlation for interval of 12-24 h (r= ... | 2015 | 26164357 |
| new porcine epidemic diarrhoea virus variant with a large deletion in the spike gene identified in domestic pigs. | from october 2013 to date, approximately 1,000 outbreaks of porcine epidemic diarrhoea virus (pedv) have occurred in japan. porcine epidemic diarrhoea with non-lethal effects in piglets was identified in tottori prefecture in october 2014. complete genome analysis revealed that the causative pathogen, tottori2, is a new pedv variant with a large (582 nt) deletion in the spike gene. phylogenetic analysis indicated that the tottori2 pedv strain might have been derived from the current pedv strains ... | 2015 | 26162305 |
| emergence of porcine epidemic diarrhea virus in southern germany. | over the last years, porcine epidemic diarrhea virus (pedv) has caused devastating enteric diseases in the us and several countries in asia, while outbreaks in europe have only been reported sporadically since the 1980s. at present, only insufficient information is available on currently circulating pedv strains in europe and their impact on the european swine industry. in this case report, we present epidemic outbreaks of porcine epidemic diarrhea in three farms in south-western germany. | 2015 | 26135732 |
| extracellular signal-regulated kinase (erk) activation is required for porcine epidemic diarrhea virus replication. | porcine epidemic diarrhea virus (pedv) is a highly enteropathogenic coronavirus of swine that causes acute enteritis with high mortality in nursery piglets. to date, the cellular factors involved in pedv replication have not been well defined. the extracellular signal-regulated kinase (erk) that serves as a critical component of cellular signal transduction pathways to modulate a variety of cellular functions has been shown to regulate several viral infections. in the present study, we found tha ... | 2015 | 26115165 |
| characterization of porcine epidemic diarrhea virus isolate us/iowa/18984/2013 infection in 1-day-old cesarean-derived colostrum-deprived piglets. | porcine epidemic diarrhea virus (pedv) was first recognized in north america in april 2013 and has since caused devastating disease. the objective of this study was to characterize disease and viral detection associated with an original north american pedv isolate inoculated in neonatal piglets. thirty-six 1-day-old cesarean-derived and colostrum-deprived piglets were randomly assigned to the control (n = 16) or challenged group (n = 20); the latter were orogastrically inoculated with 1 ml of us ... | 2016 | 26113613 |
| complete genome sequences of two genetically distinct variants of porcine epidemic diarrhea virus in the eastern region of thailand. | porcine epidemic diarrhea virus (pedv) has continued to cause sporadic outbreaks in thailand since 2007. previously, pedv in thailand was a new variant containing an insertion and deletion in the spike gene. herein, full-length genome sequences are reported for two variants of pedv isolates from pigs displaying diarrhea in thailand. | 2015 | 26112783 |
| immunoprophylactic effect of chicken egg yolk antibody (igy) against a recombinant s1 domain of the porcine epidemic diarrhea virus spike protein in piglets. | porcine epidemic diarrhea virus (pedv) is a highly contagious enteric pathogen of swine causing high mortality rates in piglets. pedv outbreaks have occurred continuously in most swine-producing asian countries and have recently emerged in the united states, leading to large economic losses for both the asian and us pig industries. the spike (s) protein of pedv consists of the s1 and s2 domains, responsible for virus binding and fusion, respectively. the involvement of the s1 domain in specific ... | 2015 | 26100403 |
| the dynamics of chinese variant porcine epidemic diarrhea virus production in vero cells and intestines of 2-day old piglets. | a severe porcine epidemic diarrhea (ped) epizootic has been affecting pigs of all ages that are characterized by high mortality among suckling piglets in china since late 2010, causing significant economic losses. obtaining a current-circulating pedv variant isolate that can grow efficiently in cell culture is prerequisite for the development of efficient vaccines. in this study, pedv strain hn1303 was isolated successfully on vero cells with supplemental trypsin, and the isolate has been serial ... | 2015 | 26091822 |
| effects of disinfection on the molecular detection of porcine epidemic diarrhea virus. | routine detection of porcine epidemic diarrhea virus (pedv) is currently limited to rt-pcr but this test cannot distinguish between viable and inactivated virus. we evaluated the capability of disinfectants to both inactivate pedv and sufficiently damage viral rna beyond rt-pcr detection. five classes of disinfectants (phenol, quaternary ammonium compound, sodium hypochlorite, oxidizing agent, and quaternary ammonium/glutaraldehyde combination) were evaluated in vitro at varying concentrations, ... | 2015 | 26072369 |
| effects of porcine epidemic diarrhea virus on porcine monocyte-derived dendritic cells and intestinal dendritic cells. | infection with porcine epidemic diarrhea virus (pedv) causes damage to intestinal epithelial cells and results in acute diarrhea and dehydration with high mortality rates in swine. dendritic cells (dcs) are highly effective antigen-presenting cells widely distributed beneath the intestinal epithelium, thus making them an early target for virus contact. dcs uptake and present viral antigens to t cells, which then initiate a distinct immune response. in this study, we investigated how attenuated p ... | 2015 | 26065617 |
| identification and pathogenicity of a variant porcine epidemic diarrhea virus field strain with reduced virulence. | since 2010, a variant porcine epidemic diarrhea virus (pedv), which causes an acute, highly contagious, and devastating viral enteric disease with a high mortality rate in suckling pigs, broke out in china and spread rapidly to neighboring countries, even to the north america. this virus gradually became the main subtype of pedv worldwide. however, there were no reports of mild pathogenicity of a variant porcine epidemic diarrhea virus in china. | 2015 | 26063495 |
| identification of the functional domain of the porcine epidemic diarrhoea virus receptor. | porcine aminopeptidase n (papn) is a functional receptor for porcine epidemic diarrhoea virus (pedv). although pedv is known to use the papn as the major receptor for cell entry, the crucial domain of the papn that interacts with the pedv is still unknown. in the present study, in order to determine the crucial domain of the papn, the extracellular domain of papn was divided into three subdomains named spa, spb and spc, based on its secondary structure. recombinant plasmid pcdna3.1 expressing sp ... | 2015 | 26044794 |
| complete genome sequence of a porcine epidemic diarrhea s gene indel strain isolated in france in december 2014. | we report the first and only case of a porcine epidemic diarrhea (ped) outbreak occurring in december 2014 in northern france, and we show using the full-length genome sequence of the french ped virus (pedv) isolate that it was a pedv indel strain close to german pedv strains recently isolated. | 2015 | 26044419 |
| complete genome characterization of porcine epidemic diarrhea virus in vietnam. | porcine epidemic diarrhea virus (pedv) first emerged in vietnam in 2009. in this study, the complete genomes of three vietnamese pedv isolates were characterized. these three isolates were isolated from 3-day-old pigs experiencing diarrhea. two isolates were from swine farms in the south, and the other was from northern vietnam. the whole genome sequences of these isolates are 28,035 nucleotides in length and have characteristics similar to those of other pedv isolates. all three vietnamese pedv ... | 2015 | 26026958 |
| the use of convalescent sera in immune-electron microscopy to detect non-suspected/new viral agents. | negative staining electron microscopy methods can be employed for the diagnosis of viral particles in animal samples. in fact, negative staining electron microscopy methods are used to identify viruses, especially in minor species and wild animals, when no other methods are available and in cases of rare, emerging or re-emerging infections. in particular, immune-electron-microscopy with convalescent sera is employed to detect etiological agents when there are undiagnosed clinical outbreaks, when ... | 2015 | 26008707 |
| complete genome sequence of a porcine epidemic diarrhea virus from a novel outbreak in belgium, january 2015. | porcine epidemic diarrhea virus (pedv) is a member of the family coronaviridae and can cause severe outbreaks of diarrhea in piglets from different age groups. here, we report the complete genome sequence (28,028 nt) of a pedv strain isolated during a novel outbreak in belgium. | 2015 | 25999551 |
| a novel pathogenic mammalian orthoreovirus from diarrheic pigs and swine blood meal in the united states. | since may 2013, outbreaks of porcine epidemic diarrhea have devastated the u.s. swine industry, causing immense economic losses. two different swine enteric coronaviruses (porcine epidemic diarrhea virus and delta coronavirus) have been isolated from the affected swine population. the disease has been reported from at least 32 states of the united states and other countries, including mexico, peru, dominican republic, canada, columbia, ecuador, and ukraine, with repeated outbreaks in previously ... | 2015 | 25991685 |
| genetic manipulation of porcine epidemic diarrhoea virus recovered from a full-length infectious cdna clone. | porcine epidemic diarrhoea virus (pedv) causes acute diarrhoea and dehydration in swine of all ages, with significant mortality in neonatal pigs. the recent rise of pedv outbreaks in asia and north america warrants an urgent search for effective vaccines. however, pedv vaccine research has been hampered by difficulties in isolating and propagating the virus in mammalian cells, thereby complicating the recovery of infectious pedv using a full-length infectious clone. here, we engineered veroe6 ce ... | 2015 | 25979733 |
| a single point mutation creating a furin cleavage site in the spike protein renders porcine epidemic diarrhea coronavirus trypsin independent for cell entry and fusion. | the emerging porcine epidemic diarrhea virus (pedv) requires trypsin supplementation to activate its s protein for membrane fusion and virus propagation in cell culture. by substitution of a single amino acid in the s protein, we created a recombinant pedv with an artificial furin protease cleavage site n terminal of the putative fusion peptide (pedv-sfcs). pedv-sfcs exhibited trypsin-independent cell-cell fusion and was able to replicate in culture cells independently of trypsin, though to low ... | 2015 | 25972540 |
| development of a real-time reverse transcription loop-mediated isothermal amplification method for the rapid detection of porcine epidemic diarrhea virus. | porcine epidemic diarrhea (ped) is an acute and highly contagious enteric disease characterized by severe enteritis, vomiting and watery diarrhea in swine. recently, the outbreak of the epidemic disease has been a serious problem in swine industry. the objective of this study is to develop a rapid, sensitive, and real-time reverse transcription loop-mediated isothermal amplification (rt-lamp) method for the detection of porcine epidemic diarrhea virus (pedv) in less equipped laboratories. | 2015 | 25972083 |
| evaluation of porcine epidemic diarrhea virus transmission and the immune response in growing pigs. | clinical disease associated with porcine epidemic diarrhea virus (pedv) infection in naïve pigs is well chronicled; however, information on endemic pedv infection is limited. to characterize chronic pedv infection, the duration of infectious virus shedding and development of protective immunity was determined. on day 0 (d0), a growing pig was challenged with pedv and 13 contacts were commingled. on d7, 9 contact pigs (principal virus group (pg)), were selected, moved to a separate room and commi ... | 2015 | 25943434 |
| comparative pathogenesis of us porcine epidemic diarrhea virus (pedv) strain pc21a in conventional 9-day-old nursing piglets vs. 26-day-old weaned pigs. | our study demonstrated potential mechanisms by which porcine epidemic diarrhea virus (pedv) infection induces greater disease severity of nursing vs. weaned conventional pigs. twenty-six-day-old weaned [pedv-inoculated (n=11); mock (n=9)] and 9-day-old nursing pigs [pedv-inoculated (n=9); mock (n=11)] were inoculated orally [8.9 log10 genomic equivalents (ge)/pig] with pc21a strain or mock (mem). pigs were monitored for clinical signs and pedv rna titers in feces and serum. for pathology and imm ... | 2015 | 25939885 |
| re-emergence of porcine epidemic diarrhea virus in the global pig population. | 2015 | 25913224 | |
| phylogenetic analysis of the spike (s) gene of the new variants of porcine epidemic diarrhoea virus in taiwan. | new variants of porcine epidemic diarrhoea virus (pedv), which emerged in taiwan in late 2013, have caused a high morbidity and mortality in neonatal piglets. to investigate the molecular characteristics of the spike (s) gene of the emerging taiwan pedv strains for a better understanding of the genetic diversity and relationship among the taiwan new variants and the global pedvs, full-length s genes of pedvs from nine 1-7 day-old piglets from three pig farms in the central and southern taiwan we ... | 2017 | 25903998 |
| porcine epidemic diarrhea virus uses cell-surface heparan sulfate as an attachment factor. | it is well known that many viruses use heparan sulfate as the initial attachment factor. in the present study, we determined whether porcine epidemic diarrhea virus (pedv), an emerging veterinary virus, infects vero cells by attaching to heparan sulfate. western blot analysis, real-time pcr, and plaque formation assay revealed that pedv infection was inhibited when the virus was pretreated with heparin (an analogue of heparan sulfate). there was no inhibitory effect when the cells were pre-incub ... | 2015 | 25896095 |
| fatal disease associated with swine hepatitis e virus and porcine circovirus 2 co-infection in four weaned pigs in china. | in recent decades, porcine circovirus 2 (pcv2) infection has been recognized as the causative agent of postweaning multisystemic wasting syndrome, and has become a threat to the swine industry. hepatitis e virus (hev) is another high prevalent pathogen in swine in many regions of the world. pcv2 and hev are both highly prevalent in pig farms in china. | 2015 | 25889526 |
| development of a nanoparticle-assisted pcr assay for detection of porcine epidemic diarrhea virus. | porcine epidemic diarrhea virus (pedv) is an important pig pathogen that can cause vomiting, diarrhea, and dehydration, leading to serious damage to the swine industry worldwide. in this study, a nanoparticle-assisted polymerase chain reaction (nanopcr) assay targeting the n gene of pedv was developed and the sensitivity and specificity were investigated. under the optimized conditions for detection of pedv rna, the nanopcr assay was 100-fold more sensitive than a conventional rt-pcr assay. the ... | 2015 | 25887451 |
| an inactivated vaccine made from a u.s. field isolate of porcine epidemic disease virus is immunogenic in pigs as demonstrated by a dose-titration. | porcine epidemic diarrhea virus (pedv), a highly pathogenic and transmissible virus in swine, was first detected in the u.s. in may, 2013, and has caused tremendous losses to the swine industry. due to the difficulty in isolating and growing this virus in cell culture, few vaccine studies using cell culture propagated pedv have been performed on u.s. strains in pigs. therefore, the objective of this study was to evaluate the humoral immune response to the selected inactivated pedv vaccine candid ... | 2015 | 25881296 |
| investigating the introduction of porcine epidemic diarrhea virus into an ohio swine operation. | porcine epidemic diarrhea virus (pedv) is a highly transmissible coronavirus that causes a severe enteric disease that is particularly deadly for neonatal piglets. since its introduction to the united states in 2013, pedv has spread quickly across the country and has caused significant financial losses to pork producers. with no fully licensed vaccines currently available in the united states, prevention and control of pedv disease is heavily reliant on biosecurity measures. despite proven, effe ... | 2015 | 25881144 |
| development and application of an elisa for the detection of porcine deltacoronavirus igg antibodies. | porcine deltacoronavirus (pdcov), also known as porcine coronavirus hku15, was first detected in north america in early 2014 and associated with enteric disease in pigs, resulting in an urgent need to further investigate the ecology of this virus. while assays detecting nucleic acids were implemented quickly, assays to detect anti-pdcov antibodies have not been available. in this study, an indirect anti-pdcov igg enzyme-linked immunosorbent assay (elisa) based on the putative s1 portion of the s ... | 2015 | 25881086 |
| a novel strain of porcine epidemic diarrhea virus in vietnamese pigs. | porcine epidemic diarrhea virus (pedv) causes severe diarrhea and dehydration in suckling pigs and has caused high rates of death among piglets and substantial economic loss in vietnam since 2009. to investigate the genotypes of prevailing pedvs, intestinal and fecal samples from piglets from central and northern vietnam were collected and analyzed. phylogenetic analysis of the nucleotide sequences of complete spike genes of pedvs from vietnam resulted in the identification of two divergent grou ... | 2015 | 25864174 |