Publications

primitive neuroectodermal tumor (neuroepithelioma) of spinal nerve root -- report of an adult case and establishment of a cell line.	a case of primitive neuroectodermal tumor arising in the cervical nerve root of a 28-year-old man is presented. histologically, the tumor was characterised by proliferation of primitive neuroectodermal cells and formation of numerous homer-wright type rosettes. a cell line (nagai line) was established from the tumor. electron microscopic examination of nagai cells revealed numerous microrosette formation with microvilli-like cytoplasmic processes projecting into the central lumina. neurosecretor ...	1979	233287
structure and function of adenovirus type 12 defective virions.	purified human adenovirus type 12 preparations contain defective virions with a lighter density. these defective virions were isolated, and their biological functions and dna were characterized. they can induce early and late antigens in infected cells and tumors in newborn hamsters with similar efficiency as complete virions. the majority of the dna molecules from light virions contain deletions mapping near 16% from the left-hand end of the genome. mechanisms for the generation of these molecu ...	1979	232175
interferon induction by adenoviruses.	all human, simian, bovine and avian adenovirus types tested so far and the canine hepatitis virus induce interferon production in chick cells. this finding indicated this property to be characteristic for viruses belonging to the adenovirus group. trypsin treatment, which had no effect upon the infectivity, diminished or eliminated the interferon-inducing abilities of crude adenoviruses, and thus the need for a trypsin-sensitive protein in interferon induction was suggested. t antigen and interf ...	1979	223139
adenoviral meningoencephalitis in a patient with lead toxicity.	adenovirus type 12 was recovered from the csf of a 36-year-old woman with adenoviral meningoencephalitis and lead toxicity. the serum level of lead was 199 micrograms/dl and the csf level was 7 micrograms/dl. after therapy with edetate disodium calcium (calcium disodium versenate), she had an uneventful recovery. the possibility of exacerbation of lead poisoning with encephalopathy due to adenovirus type 12 meningoencephalitis is raised.	1979	222248
adenovirus type 12 gene 401 function and maintenance of transformation.	rat (3y1) and hamster embryo brain cells were transformed by wild-type adenovirus type 12 or the dna-minus temperature-sensitive mutant ts401. the ts401-transformed 3y1 cells, but not the wild-type transformants, displayed a temperature-sensitive response with respect to the following characteristics of the transformed phenotype: morphology, saturation density, growth rate, cloning in soft agar, colony formation on plastic at low cell densities in 1% serum medium, and the t antigen(s). temperatu ...	1979	219220
cell-mediated immunity assayed by 51cr release test in mice infected with mouse adenovirus.	immune spleen cells (isc) from mice immunized with a sublethal dose of mouse adenovirus (m-ad) were shown by the 51cr release test to be cytotoxic to target mouse embryonic cells or lymphoid cells infected with m-ad. the number of isc required for release of statistically significant amounts of 51cr from target cells varied from one sample to another, ranging from 5 to greater than or equal to 30 isc per target cell. when 24-h-infected mouse embryonic cells were used as targets, the release of 5 ...	1978	208968
promotion of incidence of adenovirus type 12 transplantable tumors by carrageenan, a specific antimacrophage agent.	carrageenan, a sulfated polygalactose with known macrophage-toxic properties, was used to ascertain the role of macrophages in resistance to adenovirus type 12 transplantable tumors. a single ip injection of 5 or 10 mg carrageenan led to increased incidence and more rapid growth of tumors in c3h mice. carrageenan was most effective if given 1 day before tumor inoculation; the effectiveness decreased with increasing intervals before or after tumor cell injection. the macrophage stabilizer poly-2- ...	1977	191626
infection of newborn taiwan monkeys (macaca cyclopis) with human adenovirus type 12 and simian virus 40.	for testing biological response of newborn taiwan monkeys to infection of human adenovirus type 12 (ad12) and simian virus 40 (sv40), 40 newborn taiwan monkeys were inoculated with ad12, ad12, plus sv40, ad12 supplemented with ad12-induced hamster tumor tissue (ad12 tumor) or control specimens (hela or african green monkey kidney cell lysate). among them 26 survived including 8 newborn monkeys inoculated with control specimens. the survivors were observed for 4 years but no tumor was produced. t ...	1975	181217
adenovirus type 12 infection of defined mouse-human hybrid cell clones.	human adenovirus type 12 does not multiply in mouse cells; only viral t-antigen is detected. mouse-human cell hybrid clones containing human chromosomes a3, b5, c7, c11, c12, d14, e17, f19 and f20, support synthesis of adenovirus dna and capsid antigens.	1976	174935
[viruses and brain tumors (author's transl)].	this brief review paper deals mainly with oncogenic dna viruses originally isolated from human patients, i.e., human adenoviruses and human papova jc viruses. human adenovirus type 12 was first isolated in 1953 in cell cultures derived from the adenoids of infected children. since then, 32 antigenic types of human adenovirus have been identified. at least eight serotypes (12, 18, 31, 3, 7, 14, 16, and 21) are now known to be capable of producing tumors in newborn rodents. a direct causal relatio ...	1975	172819
control of nuclear division in sv40 and adenovirus type 12 transformed mouse 3t3 cells.	untransformed, non-tumorigenic mouse cells respond to cytochalasin b (cb) with limited nuclear division. balb/c mouse embryo fibroblasts (mef) and both balb/c 3t3 and swiss 3t3 cells become binucleated in the presence of cb and cells with three or more nuclei are very rare or undetectable. mef are diploid (40 chromosomes) and 3t3 cells are subtetraploid (74-76 chromosomes). transformation of mef by sv40 produces a dramatic change in response to cb. these cells, which contain sv40 t-antigen, beco ...	1975	166945
fetal antigens associated with adenovirus 12 mouse tumors.	the fetal antigen(s) of c3h/bfb/ki mouse tumor cells induced by adenovirus type 12 was examined by transplantation resistance, local adoptive transfer and in vitro cell-mediated microcytotoxicity tests. mice immunized with 8 to 9-day gestation fetal cells showed weak resistance to the challenge of small numbers of tumor cells, but immunization with 13 to 14-day gestation fetal cells did not confer transplantation immunity. local adoptive transfer of multiparous pregnant mouse spleen cells mixed ...	1976	138333
target cells of human adenovirus type 12 in subtentorial brain tissue of newborn mice. ii. cytomorphologic and immunofluorescent microscopic studies in vitro.	the normal subtentorial brain cells of newborn mice (c3h/bifb/ki) were cultured by the monolayer culture technique. the first subculture was inoculated with human adenovirus type 12 (ad 12) in an attempt to achieve the transformation of the brain cells. normal cultured cells were morphologically divided into astrocytic cells (ac-1 cells and ac-2 cells), immature brain cells including undifferentiated cells (ud cells) and glioblastic cells (gb cells), and fibroblastic cells (fb cells). fluorescen ...	1976	137655
target cells of human adenovirus type 12 in subtentorial brain tissue of newborn mice. i. cyto-histomorphologic and immunofluorescent microscopic studies.	human adenovirus type 12 (ad 12) was inoculated through subtentorial route into inbred newborn mice (c3h/bifb/ki), and sequential changes of the brain and tumor induction were examined by histological and immunofluorescent methods. two days after virus inoculation, ad 12 specific tumor antigen (fluorescent t-antigen) appeared in the cells of ependymal and subventricular matrix layers, choroid plexuses and leptomeninges in the subtentorial as well as the supratentorial brains. after 10 days, thes ...	1976	136869
in vitro studies on target cells of oncogenic adenoviruses in hamster brain. ii. in vitro transformation of brain cells of hamsters at various ages by human adenovirus type 12.	in vitro transformations of brain cells of hamsters of various ages were examined after the administration of human adenovirus type 12 (ad 12) to determine the type and origin of the target cell. hamster brain cells at all examined ages were transformed by ad12. although the virus was not isolated, virus specific tumor antigen was demonstrated in the transformed cells. the histological features of tumors that developed by transplantation of transformed cells closely resembled ad12-induced brain ...	1975	132084
differential cell membrane labilizing effect of adenovirus type 5 and 12 observed during productive and abortive infection.	culture fluid of human epitheloid (hep-2) cells was examined for extracellular lactate dehydrogenase activity as an indicator of cell damage during a 48 h period in which virus replication and changes in cell morphology occurred. uninfected and adenovirus type 5-infected cells had the same levels of extracellular enzyme activity both before and after the appearance of morphological changes in cells due to virus infection, whereas adenovirus type 12-infected cells showed increased extracellular e ...	1979	119136
adenovirus type 12 plaque formation in vero cells.		1979	112357
revertants of adenovirus type-12-transformed hamster cells have lost part of the viral genomes.		1979	90022
t antigen synthesis and resistance to interferon in human adenovirus type 12 infected chick cells.	tye specific t antigen formation has been demonstrated in primary and secondary chick embryo cells (cec) infected with adenovirus type 12. the frequency of cells synthesizing t antigen was closely dependent on the multiplicity of infection (moi). at a moi of 2.85 tcd50 per cell, t antigen was formed in 50% of cells. cycloheximide inhibited t antigen formation while cytosine arabinoside had no such effect. cec infected with adenovirus 12 produced interferon and t antigen, both appearing early and ...	1977	23662