Publications

electron microscopy of the segmented rna genome ofla crosse virus: absence of circular molecules.	the three species of single-stranded rna present in la crosse virus were examined in the electron microscope. because large amounts of contaminating cellular dna are copurified with the virus despite extensive attempts to purify the virus, it was necessary to use procedures that eliminated the bulk of this dna before the viral rna was analyzed. when this was done, the modal lengths of la crosse virus rna were 0.4, 2.0, and 3.1 mum. these lengths correspond well to their known molecular weights o ...	1977	853565
the virus particle nucleic acids and proteins of four bunyaviruses.	the structural polypeptides of five bunyaviruses, snowshoe hare, lumbo and la crosse viruses (members of the california encephalitis subgroup of bunyaviruses), bunyamwera and main drain viruses (members of the bunyamwera subgroup of bunyaviruses), have been compared by polyacrylamide-sds gel electrophoresis. each virus was found to possess three major structural polypeptides, two glycoproteins (g1 and g2), and one nucleocapsid protein (n). although the sizes of the g1 polypeptides (mol. wt. appr ...	1977	839199
congenital abnormalities in newborn lambs after infection of pregnant sheep with akabane virus.	akabane virus (a bunyavirus) has been associated with epizootics of congenital deformities in cattle, sheep, and goats. experimental studies using mouse-adapted virus inoculated intravenously into pregnant sheep gave an inapparent infection. neutralizing antibodies were detected on day 5, and peaks in the titer were seen at days 10 and 48. ewes infected at day 30 to 36 of pregnancy produced five (31% incidence) deformed lambs. sera from four of these possessed neutralizing antibodies to akabane ...	1977	832900
[demonstration of circular ribonucleic acid in the lumbo virus (bunyavirus)].	electron microscopy study of rna extracted from lumbovirus shows the existence of circular molecules, which are not susceptible to thermal or chemical denaturation. these facts suggest that the rna is present inside the virion in a covalently closed circular form.	1976	820471
[circular ribonucleoproteins in the virus lumbo (bunyavirus)].		1975	808354
[ultrastructure, development and nucleic acid of the s virus from the decapod crustacea macropipus depurator l].	the "s" crabe virus recently isolated from macropipus depurator (decapoda) has been purified and its nucleic acid has been recognized as a dna. the visualization of the structure and of several phases of the cycle of the virus permits to situate this virus near the bunyavirus and paramyxovirus groups.	1975	808338
[rna polymerase activity associated with a bunya virus (lumbo)].	rna dependent rna polymerase has been demonstrated in purified lumbo virus (bunyavirus) which contains a single stranded segmented rna. divalent cations (mn++ and mg++) are required for optimal in vitro activity. reaction products can be specifically annealed with the viral genome.	1975	808281
seriologic and vector comparisons of rift valley virus with other bunyaviruses.		1978	752702
genetic capabilities of bunyaviruses.		1978	752701
serological comparison of aino and samford viruses in simbu group of bunyaviruses.		1978	739909
small viral rna segment of bunyaviruses codes for viral nucleocapsid protein.	tryptic peptide analyses have been undertaken on the nucleocapsid (n) protein of snowshoe hare (ssh) and la crosse (lac) bunyaviruses. similar analyses have been performed on the n proteins of two recombinant viruses which have the large/medium/small rna genome configurations: ssh/lac/lac and ssh/lac/ssh. the results provide conclusive evidence that the s rna of bunyaviruses codes for the the viral n protein.	1978	702656
transmission of northway and st. louis encephalitis viruses by arctic mosquitoes.	transmission of a canadian arctic isolate of northway virus has been demonstrated after incubation of arctic aedes communis mosquitoes at 13 degrees c for 27 days after intrathoracic injection of 300 plaque forming units of virus. replication has also been demonstrated after intrathoracic injection of domestic a. aegypti mosquitoes of this virus. virions of northway virus, 84--92 nm diameter were morphologically typical of a bunyavirus after propagation in salivary glands of a. communis or in ti ...	1978	687117
antibody response to arboviruses. absence of increased response in amyotrophic lateral sclerosis and multiple sclerosis.	complement fixation and hemagglutination imhibition tests were conducted on the serums of patients with amyotrophic lateral sclerosis and multiple sclerosis using a variety of arboviral antigens. seventy-eight complement fixation and 15 hemagglutination-inhibition viral antigens were used representing togaviruses, orbiviruses, rhadoviruses, bunyaviruses, arenaviruses, and several ungrouped agents. the serological results did not indicate any relationship between these viruses and either amyotrop ...	1978	666595
formation of recombinants between snowshoe hare and la crosse bunyaviruses.	wild-type recombinants were obtained at high frequency from coinfections of bhk cells involving temperature-sensitive, conditional-lethal mutants of snowshoe hare (ssh) and la crosse (lac) bunyaviruses. analyses of two of the recombinants indicated that they have the genome compositions ssh/lac/ssh and ssh/lac/lac for their respective l, m, and s virion rna species. this evidence, together with that for the genetic stability of the recombinants, indicates that they were derived by segment reasso ...	1977	592468
three unique viral rna species of snowshoe hare and la crosse bunyaviruses.	two-dimensional gel electrophoreses of rnase t1-derived oligonucleotides of the three individual rna segments of the bunyavirus snowshow hare virus indicate that its three rna segments possess distinct nucleotide sequences. the fingerprints of the rna species of snowshoe hare virus differ from those of the antigenically closely related la crosse virus. three viral rna species have been identified in preparations of melao and trivittatus as well as snowshoe hare, lumbo, and la crosse bunyaviruses ...	1977	559104
recombination between snowhoe hare and la crosse bunyaviruses.	we have previously reported heterologous genetic recombination resulting from crosses involving temperature-sensitive (ts) mutants of la crosse (lac) group ii and snowshoe hare (ssh) group i ts mutants (j. gentsch, l. r. wynne, j. p. clewley, r. e. shope, and d. h. l. bishop, j. virol. 24:893-902, 1977). from those crosses two reassortant viruses having the large/medium/small viral rna segment genotypes of ssh/lac/ssh and ssh/lac/lac were obtained. in this study it has been found that the recipr ...	1979	513193
genome complexities of the three mrna species of snowshoe hare bunyavirus and in vitro translation of s mrna to viral n polypeptide.	the genome complexities of the principal intracellular viral complementary rna species of the snowshoe hare bunyavirus have been analyzed by duplex analyses involving hybridization of complementary rna to individual 32p-labeled viral rna species (large, l; medium, m; and small, s), recovery of nuclease-resistant duplexes, and determination of the oligonucleotide fingerprints of the protected 32p-labeled viral sequences. the result for the m rna (which codes for the glycoproteins g1 and g2; j. r. ...	1979	513192
in vivo transcription and protein synthesis capabilities of bunyaviruses: wild-type snowshoe hare virus and its temperature-sensitive group i, group ii, and group i/ii mutants.	the in vivo primary and secondary transcription capabilities of wild-type snowshoe hare (ssh) virus and certain of its temperature-sensitive (ts) mutants have been analyzed. the results obtained agree with in vitro studies (bouloy et al., c.r. acad. sci. paris 280:213-215, 1975; m. bouloy and c. hannoun, virology 69:258-264, 1976; m. ranki and r. pettersson, j. virol. 16:1420-1425, 1975) which have shown that bunyaviruses are negative-stranded rna viruses with a virion rna-directed rna polymeras ...	1979	480477
m viral rna segment of bunyaviruses codes for two glycoproteins, g1 and g2.	tryptic peptide digests of the two viral glycoproteins (g1 and g2) of snowshow hare (ssh) virus, la crosse, la crosse (lac) virus, and an ssh/lac recombinant virus which has a large (l)/medium (m)/small (s) rna segment genome composition of ssh/lac/ssh were analyzed by ion-exchange column chromatography. the analyses prove that the m rna species of bunyaviruses codes for the two viral glycoproteins.	1979	480466
heterologous interference in aedes albopictus cells infected with alphaviruses.	maximum amounts of 42s and 26s single-stranded viral rna and viral structural proteins were synthesized in aedes albopictus cells at 24 h after sindbis virus infection. thereafter, viral rna and protein syntheses were inhibited. by 3 days postinfection, only small quantities of 42s rna and no detectable 26s rna or structural proteins were synthesized in infected cells. superinfection of a. albopictus cells 3 days after sindbis virus infection with sindbis, semliki forest, una, or chikungunya alp ...	1979	480461
a comparison of la crosse virus isolated obtained from different ecological niches and an analysis of the structural components of california encephalitis serogroup viruses and other bunyaviruses.	analyses of the oligonucleotide fingerprints of the three genome ribonucleic acid (rna) species of 11 isolates of la crosse (lac) virus, obtained from various ecological niches in the northern united states and compared to those of prototype lac virus, showed that in each place from which these isolates were obtained lac variants and varieties were present with related, but distinguishable, nucleotide sequences for their large, medium, or small rna species. the rna genomes of prototypes trivitta ...	1979	453439
study of arboviruses in romania (1958--1978). results obtained in the "stefan s. nicolau" institute of virology.	the results obtained in the institute of virology by the study of arboviruses in romania are reviewed. isolation of tick-borne encephalitis virus and detection of specific serum antibodies in humans, animals and migratory birds demonstrate its presence on the territory of romania. serum antibodies could also be detected to other three flaviviruses (west nile, ntaya, dengue types 1, 2, 3), though none of them could be isolated in our country. however, isolation of kemerovo and tahyna viruses and ...	1979	392912
genetic potential of bunyaviruses.		1979	387343
the first isolations of eastern encephalitis, group c, and guama group arboviruses from the peruvian amazon region of western south america.	two strains of eastern encephalitis (ee) virus were isolated in the amazon region of peru near pucallpa, loreto department, using sentinel hamsters. ee virus antibodies were found in healthy horses at both pucallpa and iquitos in the same department. fourteen group c and four guama group arboviruses were recovered from sentenel hamsters and mosquitoes near iquitos. the group c agents were caraparu-ossa, marituba, and oriboca-itaqui viruses, and the guama group agents were bimiti virus. besides p ...	1975	238693
biochemical studies on the phlebotomus fever group viruses (bunyaviridae family).	analyses of the virion polypeptides and genomes of several phlebotomus fever group viruses, karimabad, punta toro, chagres, and the sandfly fever sicilian serotype viruses, have established that they are biochemically similar to the accepted members of the bunyaviridae family. like snowshoe hare virus (a member of the california serogroup of the bunyavirus genus of the bunyaviridae family), karimabad, punta toro, chagres, and the sandfly fever sicilian serotype viruses all have three viral rna s ...	1979	225523
investigations on the presence of antibodies to alphaviruses, flaviviruses, bunyavirus and kemerovo virus in humans and some domestic animals.	the presence of antibodies to some alphaviruses, flaviviruses, bunyaviruses and orbiviruses was studied in the sera from humans and domestic animals of biotopes "c" and "t" in the south-east of romania. specific antibodies were found in humans to 3 alphaviruses (eastern equine encephalitis, western equine encephalitis and sindbis), in a very low proportion, and to 3 flaviviruses (tickborne encephalitis, west nile and ntaya), at a much higher percentage. very low percentages of antibodies to buny ...	1978	209613
induction of interferon by group c arboviruses. brief report.		1978	104697
discontinuous counter-immunoelectrophoresis in the study of viruses.	a method for the use of the discontinuous counter-immunoelectropnoresis (dcie) technique in the study of viruses of the major taxonomic groups is reported. the method was successfully employed in detection of antigen of viruses in the bunyavirus, bunyavirus-like, flavivirus, orbivirus, and rhabdovirus groups, and/or of antibody to these agents. although less sensitive than the complement fixation test, the dcie test was found to be rapid and in some cases more specific in differentiating related ...	1978	97243
bunyavirus development in arctic and aedes aegypti mosquitoes as revealed by glucose oxidase staining and immunofluorescence.	northway virus replication has been detected in salivary glands of wild-caught culiseta inornata and aedes communis mosquitoes from the western canadian arctic after incubation at 4 degrees c for 9 to 11 months, and after incubation at 13 degrees c for 3 to 4 months after they received virus by oral ingestion or intrathoracic injection. aedes hexodontus supported northway virus replication after incubation at 13 degrees c for one month after intrathoracic injection. aedes aegypti supported north ...	1979	44464
tonate and guama-group viruses isolated from mosquitoes in both a savannah and coastal area in surinam.	isolation of arboviruses was attempted from october 1972 till the end of 1973 from mosquitoes caught in both a savannah forest near powaka and a coastal area near "fernandesweg" in surinam. two types of arbovirus were isolated: eleven strains of tonate and ten strains of guama-group. in the savannah area ten strains of tonate and five of guama-group were isolated, whereas in the coastal area the numbers were one and five, respectively. all strains in the savannah area were isolated from pools of ...	1979	41349
razdan virus, a new ungrouped bunyavirus isolated from dermacentor marginatus ticks in armenia.	a virus, designated razdan, was isolated from dermacentor marginatus ticks in the armenian s.s.r. in 1973. the complement fixation tests revealed no antigenic relationships to 74 tick-borne arboviruses. the size of the virus is about 100 nm; it agglutinates goose erythrocytes at ph 5.5--7.0, is pathogenic for newborn, 14-day-old and adult white mice and multiplies in primary and continuous cell cultures. morphological properties of the virus permit its classification as a member of the family bu ...	1978	35950
experimental infection of putorius eversmanni polecats and martes foina martens with tahyna virus.	three putorius eversmanni pole-cats and two martes foina martens aged about 9 months were subcutaneously infected with about 260 suckling mouse ld50 of the extraneurally passaged "236" strain of tahyna virus (california group, genus bunyavirus). viraemia with maximal titres of 1.32 (pole-cats) and 1.28 (martens) dex intraperitoneal (i.p.) mouse ld50/0.02 ml was demonstrated from 48 to 96 hr after inoculation (p.i.). by the plaque-reduction neutralization test, seroconversion was demonstrated 15 ...	1978	35949
khasan virus, a new ungrouped bunyavirus isolated from haemaphysalis longicornis ticks in the primorie region.	strain leiv-776p, possessing no haemagglutinating activity was isolated in 1971 in the primorie region (u.s.s.r.) from haemaphysalis longicornis neumann 1091 tick. complement fixation (cf) tests revealed no antigenic relationship with 24 antigenic groups of arboviruses or 21 ungrouped viruses isolated from ticks. the virus isolate contains rna and is relatively sensitive to ether and sodium deoxycholate; it is pathogenic for suckling mice and two-week-old white mice by the intracerebral route, a ...	1978	27977
bunyavirus isolations from mosquitoes in the western canadian arctic.	strains of california encephalitis virus (snowshoe hare subtype) were isolated from 8 of 475 pools comprising 23 747 unengorged female mosquitoes of five species collected at three of six locations throughout the mackenzie valley of the northwest territories, canada, from latitudes 60 to 69 degrees n between 10 and 24 july 1976. minimum field infection rates included 1:2734 for aedes communis, 1:256 to 1:3662 for a. hexodontus and 1:911 to 1:1611 for a. punctor. northway virus was also isolated ...	1977	18541
mosquito-borne arboviruses in arctic america.	mosquito-borne arboviruses are prevalent throughout subarctic regions of canada and alaska, principally in the boreal forest extending between latitudes 53 and 66 degrees n, but they have been identified in tundra regions as far north as 70 degrees n. all mosquito-borne agents have been bunyaviruses, comprising principally the snowshoe hare subtype of california encephalitis (ce) virus, but also northway virus. mosquito vectors comprise several aedes species and culiseta inornata, all of which h ...	1975	1602